Environmental Technology & Innovation最新文献

{"title":"High-risk and virus-linked antibiotic resistome in swine farms: metagenomic profiling of cross-species transmission risks among humans, pigs, and soil","authors":"Wentao Lyu ,&nbsp;Qu Chen ,&nbsp;Xingning Xiao ,&nbsp;Yuanxiang Jin ,&nbsp;Wen Wang ,&nbsp;Yingping Xiao ,&nbsp;Hua Yang ,&nbsp;Lingyan Ma","doi":"10.1016/j.eti.2025.104368","DOIUrl":"10.1016/j.eti.2025.104368","url":null,"abstract":"<div><div>The rapid acceleration of antimicrobial resistance (AMR) has emerged as a critical and pressing global threat. The role of viromes in disseminating antibiotic resistance genes (ARGs) across human-livestock-environment interfaces remains poorly characterized, despite its critical implications for AMR containment. In the present study, we conducted antibiotic resistome and viral metagenomic sequencing on paired samples from a swine farm ecosystem: pig feces, farm soil, worker stools, and non-exposed human controls, to outline the distribution characteristics of risk ARGs, viromes and virus-associated ARGs. Firstly, farmworkers exhibited significantly greater abundance of high-risk Rank 1 ARGs, particularly β-lactamase genes compared to controls, while soil displayed markedly lower ARG diversity. Next, we identified 138 viral OTUs carrying ARGs (predominantly Siphoviridae, Myoviridae and Podoviridae), with 67 % targeting pathogenic hosts including <em>Escherichia coli</em>. These viral carriers mediated resistance to clinically important antibiotics, such as tetracycline, aminocoumarin, and fosfomycin. Additionally, it was also found that occupational contact increases the diversity of ARGs in the viromes, suggesting phage-mediated ARG transfer is amplified by farm exposure. Collectively, this study provides a more comprehensive characterization of high-risk ARGs and virus-associated ARGs in swine farm systems, highlighting the incorporate virome surveillance in the spread of AMR.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104368"},"PeriodicalIF":6.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing tea plantation productivity: Magnesium-modified tea pruning litter biochar enhances soil quality and tea aroma profiles","authors":"Wenbin Liu ,&nbsp;Yubo Luo ,&nbsp;Xiangzhen Zhu ,&nbsp;Dubin Dong ,&nbsp;Mei Wang ,&nbsp;Jiawei Ma ,&nbsp;Zhengqian Ye ,&nbsp;Dan Liu","doi":"10.1016/j.eti.2025.104375","DOIUrl":"10.1016/j.eti.2025.104375","url":null,"abstract":"<div><h3>Purpose</h3><div>Soil acidification and magnesium (Mg) deficiency severely limit tea productivity and quality. This study investigated the potential of Mg-modified biochar, derived from tea pruning waste, to remediate acidic soils and enhance tea plant performance.</div></div><div><h3>Methods</h3><div>A 90-day pot experiment was conducted with four treatments: control (CK), Mg sulfate fertilizer (FC), tea pruning litter biochar (BC), and Mg-modified tea pruning litter biochar (BCY). The study evaluated the effects on soil chemical properties (pH, nutrients, cations), plant growth parameters, and tea quality indicators, including biochemical and aroma profiles.</div></div><div><h3>Results</h3><div>Both BC and BCY significantly increased soil pH by 0.27–0.39 units (<em>p</em> &lt; 0.05). Among treatments, BCY showed the most pronounced effects, improving soil organic matter (22.93 g kg⁻¹), available phosphorus (by 84 %), cation exchange capacity (by 76 %), and Mg²⁺ (by 519 %) levels, while reducing Al³⁺ and H⁺ concentrations by 59 %. In addition, BCY enhanced microbial activity and carbon (C) metabolism. Notably, tea plants treated with BCY exhibited greater height, biomass, and improved nutrient uptake (nitrogen, potassium, sodium, and Mg). Correspondingly, tea quality was significantly improved, with elevated levels of polyphenols, amino acids, caffeine, and aromatic compounds.</div></div><div><h3>Conclusions</h3><div>Mg-modified biochar derived from tea pruning residues effectively ameliorates acidic soils, alleviates Mg deficiency, and stimulates microbial activity in tea plantations. These soil improvements result in better plant growth, nutrient uptake, and ultimately lead to superior tea quality and yield. These findings support its potential as a sustainable soil amendment in tea plantation management.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104375"},"PeriodicalIF":6.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of soil bacterial community composition and function to Gastrodia elata Bl. consecutive monoculture: Insights from metagenomic analysis","authors":"Xing Ye ,&nbsp;Jiao Xu ,&nbsp;Qingsong Yuan ,&nbsp;Ye Yang ,&nbsp;Lanping Guo ,&nbsp;Yanhong Wang ,&nbsp;Tao Zhou","doi":"10.1016/j.eti.2025.104372","DOIUrl":"10.1016/j.eti.2025.104372","url":null,"abstract":"<div><div><em>Gastrodia elata</em> Bl. monoculture practice commonly contributes to yield decline over consecutive planting cycles. Given the critical role of soil microbiota in soil biogeochemical cycling, understanding microbiome shifts under consecutive cultivation could provide insights for yield sustainability. However, knowledge gap remains regarding which microbial taxa response to consecutive monoculture and how they respond. Thus, the composition and function of soil bacterial communities in a field where <em>G. elata</em> had been cultured for one year (CC1), consecutively cultured for 2 years (CC2), and no-tillage control (CK) were compared. Both CC1 and CC2 increased the soil bacterial richness and evenness, as evidenced by ACE (<em>p</em> = 0.0025) and Simpson (<em>p</em> = 0.006) indices. Principal coordinates analysis revealed distinct clustering of bacterial community structures, with CC1 and CC2 samples significantly separated from control (CK) samples (R=0.636, <em>p</em> = 0.001). Comparative analysis showed that CC1 and CC2 treatments significantly reduced the relative abundances of <em>Arthrobacter</em> and <em>Candidatus_Acidoferrum</em> compared to CK, while increasing the abundances of <em>Afipia, Alloacidobacterium, Bradyrhizobium</em> and <em>Burkholderia</em>. Soil pH (R=0.4721, <em>p</em> = 0.009), alkali-hydrolytic nitrogen (AN; R=0.4855, <em>p</em> = 0.012), available phosphorus (AP; R=0.4474, <em>p</em> = 0.014), and total potassium (total K; R=0.5149, <em>p</em> = 0.005) emerged as the key edaphic factors shaping bacterial community structure and composition. <em>G. elata</em> cultivation, either single-cycle or consecutive, significantly reduced the abundance of GT41, GT83, GH109, GH33, and GT5 (<em>p</em> &lt; 0.05). Conversely, AA3_2, GH13_11, GH13_16, GH39, GH51, and GH103 showed the opposite trend. Our findings highlight the complexity of microbial feedback mechanisms and soil-microbiome interactions under <em>G. elata</em> monoculture conditions<em>.</em></div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104372"},"PeriodicalIF":6.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile synthesis of oxygen vacancy-rich BiMnx composites for enhancing antibiotic removal driven by LED visible light","authors":"Jing Wang ,&nbsp;Siyu Zhang ,&nbsp;Lidong Kou ,&nbsp;Kunzhen He ,&nbsp;Qingyuan Li ,&nbsp;Chaojun Wu ,&nbsp;Xing Xing ,&nbsp;Yanjin Wang","doi":"10.1016/j.eti.2025.104374","DOIUrl":"10.1016/j.eti.2025.104374","url":null,"abstract":"<div><div>NaBiO₃ is a low-cost photocatalyst with good performance for organic removal. Oxygen vacancies (OVs) play crucial roles in mediating oxygen activation and electron-hole separation during visible light-driven photocatalysis. In this study, Mn ions were doped into the commercial NaBiO₃ by a facile in-situ technique to construct OVs for enhancing sulfamethazine (SMT) removal. By doping 0.001–0.01 mmol of Mn^2 + ions, abundant OVs were formed on the initial NaBiO₃ surface, endowing the as-prepared BiMn_x composites (e.g. BiMn_0.001) with a fast kinetic (first-order rate constant <em>k</em> 0.68 h⁻¹) and a high efficiency (&gt;99 % within 6 h) for SMT degradation. Excellent removal of SMT could be attained across a broad pH range (i.e. pH₀ 5–9) using BiMn_0.001 as the photocatalyst, which could harvest full-spectrum LED white light and showed anti-interference to common water co-existent constituents. Increasing solution temperature was also beneficial for SMT removal. Reactive species including ¹O₂, holes and electrons played dominant roles for SMT removal. Based on HPLC-MS, the degradation intermediates were identified, and a degradation pathway for SMT was formulated in the BiMn_x/LED system. The results may shed some light for constructing OVs on catalyst surfaces and preparing LED visible light-responsive photocatalysts for antibiotic removal.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104374"},"PeriodicalIF":6.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of propionate degradation in anaerobic reactors: Roles of sludge retention time and organic carbon composition across different operational configurations","authors":"Tingxia Liu ,&nbsp;Chuanqi Liu ,&nbsp;Huanhuan Chang ,&nbsp;Guangxue Wu","doi":"10.1016/j.eti.2025.104370","DOIUrl":"10.1016/j.eti.2025.104370","url":null,"abstract":"<div><div>Propionate accumulation is a challenge in anaerobic digestion due to the syntrophic lifestyle, slow growth rate, and environmental sensitivity of syntrophic propionate-oxidizing bacteria. The effects of sludge retention times (SRTs; i.e., 10 and 25 days, and without desludge) and carbon source compositions (a mixture of ethanol/acetate/propionate or sole propionate) on propionate degradation and microbial community structure were examined in sequencing batch reactors (SBRs) and continuous flow reactors (CFRs). SBRs outperformed CFRs in methanogenic propionate degradation, with nearly complete chemical oxygen demand (COD) removal achieved by day 13 (SBR with a mixture of ethanol/acetate/propionate) and day 41 (SBR with sole propionate), compared to day 54 in the CFR with sole propionate. The COD removal efficiency in the CFR with a mixture of ethanol/acetate/propionate stabilized at approximately 70 %. Reactors with a 10-day SRT were unable to efficiently remove propionate across different operational modes and carbon sources. Batch experiments showed that reactors utilizing mixed carbon sources exhibited shorter lag phases, increased acetate degradation activities, and higher maximum methane production rates compared to those using the sole propionate. SBRs enriched <em>Syntrophobacter</em> (16.0 %–22.8 %) and <em>Desulfobulbus</em> (4.1 %–5.0 %), whose relative abundances in CFRs were only 4.1 %–13.5 % and 1.0 %–1.2 %, respectively. Additionally, the relative abundance of genes involved in propionate oxidation increased by 9.7 %–47.0 % in SBRs compared to CFRs. A strategy involving the utilization of long SRTs, SBR operation mode, and mixed carbon sources was proposed to improve system startup and propionate removal in anaerobic reactors.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104370"},"PeriodicalIF":6.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, antioxidant properties and transcriptome analysis of selenium nanoparticles biosynthesized by the high selenite tolerance strain Halomonas sp. SF2000","authors":"Shoufeng Liu,&nbsp;Qiuya Gu,&nbsp;Jie Gao,&nbsp;Zehao Li,&nbsp;Xiaobin Yu","doi":"10.1016/j.eti.2025.104373","DOIUrl":"10.1016/j.eti.2025.104373","url":null,"abstract":"<div><div>Microbial synthesis of selenium nanoparticles (SeNPs) has garnered significant attention in recent years due to its eco-friendly and sustainable nature. Numerous microorganisms possess the capability to reduce ionic selenium into SeNPs, a process often associated with microbial detoxification mechanisms. In this study, a strain exhibiting exceptionally high selenite tolerance (up to 1200 mM) was isolated from selenium-enriched organic fertilizers, representing the highest tolerance level reported to date. The strain was identified as <em>Halomonas</em> sp. SF2000. Furthermore, the selenite reduction capability of SF2000 was systematically investigated. The strain reduced 10 mM selenite completely within 60 h. Electron microscopy revealed that the synthesized SeNPs were spherical and predominantly localized extracellularly. Characterization showed that the nanoparticles had a zeta potential of −47.8 mV, confirming the stability of the system. Fourier transform infrared (FTIR) spectroscopy analysis provided insights into the surface functional groups of the nanoparticles. Additionally, the SeNPs exhibited significant antimicrobial activity, free radical scavenging ability, and antioxidant capacity, confirmed by <em>in vitro</em> assays. Transcriptomic analysis revealed that low selenite concentrations primarily upregulated genes associated with reduction activity, while high concentrations also induced the expression of resistance-related genes. This dual regulatory mechanism underpins the exceptional selenite tolerance of SF2000. Notably, this study provides the first report on selenite reduction capability by <em>Halomonas</em> sp., establishing a theoretical foundation for the potential application of SF2000 in environmental remediation, advanced materials, and functional health products.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104373"},"PeriodicalIF":6.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic and environmental feasibility assessment of emerging biometallurgical recycling methods for spent electric-vehicle batteries","authors":"Xumei Zhang ,&nbsp;Xiaoming Bai ,&nbsp;Feng Ma ,&nbsp;Yan Wang","doi":"10.1016/j.eti.2025.104369","DOIUrl":"10.1016/j.eti.2025.104369","url":null,"abstract":"<div><div>Efficient recycling of valuable metals from spent electric vehicle (EV) batteries is critical for improving the stability of the raw material supply and achieving sustainable development. Currently, the predominant methods for recycling spent EV batteries include pyrometallurgical and hydrometallurgical recycling, but neither is efficient in saving energy and mitigating pollution. Emerging biometallurgical recycling methods provide a promising alternative to conventional techniques by eliminating the need for additional toxic chemicals, thus reducing operational costs and pollution emissions. Despite their potential advantages for metal recovery from spent EV batteries, they have not been widely adopted in the industry due to uncertain economic and environmental feasibility. Thus, this study presents a comprehensive feasibility assessment of biometallurgical recycling methods for spent EV batteries based on economic benefits and environmental impacts. The economic benefits are analyzed using an integrated system dynamics (SD) and cost-benefit analysis (CBA) approach to address system complexity and the time delay impact. Moreover, the life cycle assessment (LCA) model estimates 12 environmental impact categories using the ReCiPe characterization method. From the economic perspective, biometallurgical methods are more economically feasible than hydrometallurgy and pyrometallurgy, with 16 % and 27 % greater profits, respectively. Sensitivity analysis indicates that profitability is prominently influenced by the market price volatility of recovered metals, especially cobalt, and battery type variability. Furthermore, higher operating efficiencies and additional cost reductions can be achieved through economies of scale, thereby enhancing recycling profitability. From the environmental perspective, biometallurgical recycling of spent EV batteries is more environmentally sustainable than conventional methods due to reduced chemical reagents and energy consumption. For instance, biometallurgical recycling has a global warming potential (GWP) of 6.26 kg CO₂ eq./kg, which can effectively reduce emissions by 6.4 and 9.5 kg CO₂ eq./kg compared to conventional methods, respectively. Overall, this work will help governments and businesses select the most sustainable recycling method, thereby promoting sustainable development of the battery industry.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104369"},"PeriodicalIF":6.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of microplastics and nanoplastics in pediatric myocardium and blood","authors":"Lizhi Lv ,&nbsp;Xinyue Lang ,&nbsp;Yuekun Sun ,&nbsp;Gang Li ,&nbsp;Yongtao Wu ,&nbsp;Zhiguang Liu ,&nbsp;Qiang Wang","doi":"10.1016/j.eti.2025.104371","DOIUrl":"10.1016/j.eti.2025.104371","url":null,"abstract":"<div><div>Microplastics and nanoplastics (MNPs) are emerging environmental contaminants with potential health implications. Previous research has detected the existence of MNPs in the bloodstream and cardiac tissue of adult individuals, yet their presence and impact in pediatric populations remain largely unexplored. This study aimed to describe the concentration of MNPs in pediatric patients’ myocardium and blood samples. A total of 27 pediatric patients, along with 3 mothers, were included, who provided 27 samples of the myocardium, 7 pairs of pre- and post-surgery venous blood samples, and 3 pairs of the placenta and umbilical cord blood samples. Quantification of 9 kinds of MNPs was achieved using Pyrolysis-gas chromatography coupled with mass spectrometry (Py-GC/MS). The Laser Direct Infrared (LDIR) system was used to measure the size of MNPs. Four types of MNPs were identified by Py-GC/MS in the myocardium, including polyethylene (PE) (27/27, 100 %), Polystyrene (PS) (26/27, 96.3 %), polypropylene (PP) (22/27, 81.5 %), and polyvinyl chloride (PVC) (23/27, 85.2 %). The median concentration of total MNPs was 38.4 (IQR 27.4, 57.0) μg/g. Patients with 0-day of age (Immediately after birth) had the highest levels of MNPs in the myocardium. Mothers’ placenta and umbilical cord blood samples had the same types of MNPs with neonates’ myocardium and pre-surgery blood. The concentration of MNPs in post-surgery blood was higher than that in pre-surgery blood (3.54 ± 1.50 μg/g vs. 7.62 ± 2.99 μg/g, <em>p</em> = 0.007). Additional investigation is required to understand the origins and metabolic networks of MNPs and establish the method by which MNPs harm the cardiovascular system of youngsters.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104371"},"PeriodicalIF":6.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of human exposure risks to perfluoroalkyl acid residues via municipal drinking water and source analysis in China","authors":"Jia Lyu ,&nbsp;Yongyan Chen ,&nbsp;Lan Zhang ,&nbsp;Xin Wen ,&nbsp;Muyesaier Tudi","doi":"10.1016/j.eti.2025.104367","DOIUrl":"10.1016/j.eti.2025.104367","url":null,"abstract":"<div><div>Perfluoroalkyl acids (PFAAs) with carboxylic or sulfonic acid groups remain major pollutants in drinking water, posing a significant health risk. To explore the spatial and seasonal trends in PFAA exposure risks, considering different industrial layouts and water consumption habits, this study quantified 11 typical PFAAs in source and drinking water samples collected during winter and summer across nine major water basins in China. PFAA exposure and associated risk were evaluated based on age- and region-specific water consumption habits, and trends in source water contamination were analyzed in relation to varying production activity profiles. Drinking water has become the dominant source of PFAA exposure, mainly due to source water pollution from PFAA-related manufacturing and consumption. Exposure to PFAAs via drinking water varied among different water basins and seasons with total PFAA concentrations ranging from 0.0175 to 22.2 ng/kg/day. A greater risk of exposure to PFAAs was observed in populations from industrialized or highly urbanised areas in eastern and southern China, with a maximum health risk index of 5.53. C₈ PFAAs, although regulated, remain the main risk component in drinking water, indicating these contaminants need to be revisited to enhance health-based drinking water safety management. Further studies on the establishment of health-based drinking water standards, water treatment technology updates, and source pollution reduction are required to control the risks of PFAA exposure in drinking water. This study provides important support for the global management of PFAA safety in drinking water.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104367"},"PeriodicalIF":6.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable valorization of tea waste by enhanced caffeine extraction via microbial-driven solid-state fermentation","authors":"Bixia Qiu ,&nbsp;Jiaying Yu ,&nbsp;Danqi Chen ,&nbsp;Yuying Zeng ,&nbsp;Chunyu Li ,&nbsp;Peirong Yu ,&nbsp;Yuexin Yang ,&nbsp;Xueqing Huang ,&nbsp;Yancui Liang ,&nbsp;Xiaohui An ,&nbsp;Zoya Okun ,&nbsp;Avi Shpigelman ,&nbsp;Yigal Achmon","doi":"10.1016/j.eti.2025.104365","DOIUrl":"10.1016/j.eti.2025.104365","url":null,"abstract":"<div><div>Solid-state fermentation (SSF) of green tea waste (GTW) and black tea waste (BTW) was evaluated to assess microbial effects on extractable caffeine content. A significant caffeine increase was observed, with GTW fermented at 45°C and 55 % water content for 6 days yielding the higher caffeine content (∼13.7134 mg/g dry mass via high-temperature extraction, ∼5.098 mg/g dry mass higher than control, unfermented GTW on day 0). SSF also enhanced caffeine extraction at room temperature with minimal solvent, offering an energy-efficient advantage. Volatile organic compounds (VOCs) analysis by the proton transfer reaction-time of a flight-mass spectrometer (PTR-ToF-MS) and microbial profiling by DNA sequencing analysis revealed key VOCs (<em>m/z</em> 59, <em>m/z</em> 61, <em>m/z</em> 89) linked to microbial activity. Dominant bacteria (<em>Bacillus, Paenibacillus</em>) and fungi (<em>Aspergillus</em>) in fermented GTW likely contributed to improved caffeine extractability. These findings highlight microbial transformations in tea waste SSF and its potential for sustainable caffeine extraction as a value-added product.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104365"},"PeriodicalIF":6.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}