Environmental Technology & Innovation最新文献

{"title":"Facile preparation of Z-scheme based graphene/TiO2/MnO2 hybrid nanomaterial: A dual application in hazardous dye and antibacterial reduction","authors":"Bhuvaneswari kandasamy ,&nbsp;Pazhanivel Thangavelu ,&nbsp;Sarun Phibanchon ,&nbsp;Rotruedee Chotigawin ,&nbsp;Taddao Pahasup-anan ,&nbsp;Surachai Wongcharee ,&nbsp;Ranjith Rajendran ,&nbsp;Thammasak Rojviroon ,&nbsp;Kowit Suwannahong","doi":"10.1016/j.eti.2025.104262","DOIUrl":"10.1016/j.eti.2025.104262","url":null,"abstract":"<div><div>This study explores the photocatalytic degradation efficacy of methylene blue (MB) dye, a prevalent water pollutant, employing graphene/TiO₂/MnO₂ (GTM) hybrid photocatalyst. The photocatalytic degradation performance against MB dye revealed that the as-prepared GTM hybrid photocatalyst exhibited superior activity, achieving a degradation efficiency of 97 % within 40 min of UV–visible light irradiation. Notably, the GTM hybrid photocatalyst demonstrated a high-rate constant (k) of 0.174 min⁻¹ , surpassing that of the bare TiO₂ (k = 0.030 min⁻¹) and MnO₂ (k = 0.047 min⁻¹) photocatalysts. Cyclic stability tests up to ten cycles confirmed the excellent durability of the GTM hybrid photocatalyst, retaining 80 % of its initial activity. Hydroxyl and superoxide radicals play a significant role in the degradation process, while the heterojunction structure significantly enhances charge separation, further improving photocatalytic efficiency. The enhanced photocatalytic performance of the GTM hybrid can be attributed to the improved charge carrier separation, augmented surface area, and 2D/2D/1D heterojunction formation. Also, the antibacterial activity of the as-prepared photocatalyst was analyzed over <em>Klebsiella pneumoniae (KP)</em> and <em>Staphylococcus aureus (SA)</em> using the agar well diffusion method. The observed results show that the GTM has effective antibacterial activity against both microorganisms. This research contributes to developing sustainable materials and processes for environmental remediation, aligning with the principles of green chemistry and promoting a cleaner, healthier environment.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104262"},"PeriodicalIF":6.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphoric acid modified biochar enhanced heavy metals passivation via accelerating humification and bioremediation in sewage sludge composting","authors":"Xiuwen Qiu ,&nbsp;Mengting Feng ,&nbsp;Ruixu Chen ,&nbsp;Jiajia Chen ,&nbsp;Xiangrong Zheng ,&nbsp;Xia Mao ,&nbsp;Wei Song ,&nbsp;Guixiang Zhou","doi":"10.1016/j.eti.2025.104275","DOIUrl":"10.1016/j.eti.2025.104275","url":null,"abstract":"<div><div>This study investigated the effects of phosphoric acid modified coconut shell biochar (PMB) on humification parameters, heavy metals bioavailability, and the structure and metabolism of bacterial community during sewage sludge composting. The results indicated that PMB addition decreased the polyphenols content and increased polyphenol oxidase (PPO) and laccase activity. At the end of composting, the humic acid (HA) concentration of PMB treatment rose by 22.19 %, and the degree of polymerization and humification index (HIX) also increased significantly, compared with control (CK) treatment. In addition, the bioavailable contents of Cu, Fe, Mn, Ni, Pb and Zn in PMB were significantly lower than CK, which decreased by 19.32 %, 35.28 %, 39.72 %, 41.99 %, 36.28 % and 32.27 %, respectively. The addition of PMB enhanced the richness and diversity of bacterial community. The abundances of sequences associated with carbohydrate metabolism, amino acid metabolism, energy metabolism and lipid metabolism in PMB were consistently higher than that in CK. Correlation analysis showed that <em>Streptomyces</em>, <em>Bacillus</em>, <em>Pseudomonas</em> and <em>Taibaiella</em> were significantly positively associated with heavy metals passivation and HA formation. These findings suggested that PMB promoted organic matter decomposition and HA formation, and decreased bioavailability of heavy metals by promoting bacterial bioremediation and HA absorption and complexation. Therefore, the addition of PMB is a suitable strategy to mitigate the environmental risk of heavy metals in sewage sludge.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104275"},"PeriodicalIF":6.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial-driven soil remediation: Comparative analysis of seven microbial agents on alachlor degradation rates and tobacco phytotoxicity recovery","authors":"Ling Liu ,&nbsp;Yu Xiang ,&nbsp;Jianling Liu ,&nbsp;Guangbin Wu ,&nbsp;Yonggang Ren ,&nbsp;Zhibo Chen ,&nbsp;Yuping Zhang","doi":"10.1016/j.eti.2025.104264","DOIUrl":"10.1016/j.eti.2025.104264","url":null,"abstract":"<div><div>The presence of alachlor residue in soil has been demonstrated to exert a deleterious effect on the subsequent cultivation of crops, resulting in substantial impairment to agricultural output. Application of alachlor at twice the recommended dose notably inhibited tobacco growth, reducing plant height to 39.68 % of the control, stem girth to 40.06 %, maximum leaf length to 45.52 %, and maximum leaf width to 42.05 %. A total of seven functional microbial agents were screened for their potential to alleviate tobacco phytotoxicity. Results demonstrated that two microbial agents, <em>Purpureocillium lilacinum</em> (PL) and <em>Pseudomonas fluorescens</em> (PF), significantly alleviated toxicity, enhancing plant height, stem girth, and leaf expansion (<em>P</em> &lt; 0.001 or <em>P</em> &lt; 0.01). This study also developed and validated an efficient method for detecting alachlor in soil, demonstrating excellent linearity (R² &gt; 0.99) with recoveries ranging from 88.98 % to 93.60 %, a limit of detection (LOD) of 0.0005 mg/kg, and a limit of quantification (LOQ) of 0.02 mg/kg. The degradation efficiency of alachlor varied among microbial agents, with PL and PF exhibiting the highest degradation rates of 93.50 ± 0.69 % and 90.80 ± 1.02 % at 70 days post-application. Microbial diversity analysis showed that PL and PF treatments increased the abundance of Actinobacteria and specific microbial genera, which facilitated alachlor degradation and improved soil ecological conditions. These findings suggest that PL and PF hold significant potential to accelerate alachlor degradation, alleviate alachlor toxicity, and enhance soil health, offering valuable insights for farmland pollution remediation.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104264"},"PeriodicalIF":6.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutral initial pH enhances the formation of humic acid by inhibiting the growth of Lactobacillus in food waste composting","authors":"Min Xu ,&nbsp;Yuquan Wei ,&nbsp;Yunfeng Chen ,&nbsp;Haibin Zhou ,&nbsp;Shuangshuang Ma ,&nbsp;Yabin Zhan","doi":"10.1016/j.eti.2025.104271","DOIUrl":"10.1016/j.eti.2025.104271","url":null,"abstract":"<div><div>Composting was the main way to treat food waste and achieve its resource utilization, but the fermentation of food waste could easily produce acid, which affects the microbial community of composting. As of now, the impact of this process on humification was still unclear. This study investigated the effects of initial pH on humic acid formation during food waste (FW) composting, as well as the microbial regulatory mechanisms under pH conditions of 5.0, 6.0 and 7.0. Our study investigated that adjusting the initial pH to 6.6, especially in 7.0, promoted total organic carbon degradation and compost maturation, as indicated by germination index and decreased electrical conductivity. However, higher pH levels also increased total nitrogen loss. Adjusting the pH to 6.0 or 7.0 facilitated the conversion of reducing sugars, amino acids and polyphenols into humic acid and promoted the formation of complex HA components. Our results also indicated that pH regulation inhibited <em>Lactobacillus</em> growth, reshaped bacterial composition, influenced amino acid and polyphenol dynamics, and ultimately enhanced humic acid formation in initial pH of 6.0, especially in initial pH 7.0. From a cost perspective, it was recommended to adjust the initial pH of food waste to 6.0 for composting.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104271"},"PeriodicalIF":6.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable biocementation of mine tailings: Reduction of urea requirements through bicarbonate-based MICP","authors":"Héctor Zúñiga-Barra ,&nbsp;Camila Pardo-Vásquez ,&nbsp;Edgar Velastegui ,&nbsp;Jimmy Anderson Martínez-Ruano ,&nbsp;Mariella Rivas ,&nbsp;David Jeison","doi":"10.1016/j.eti.2025.104255","DOIUrl":"10.1016/j.eti.2025.104255","url":null,"abstract":"<div><div>Microbially induced calcite precipitation (MICP) has emerged as a promising technique to contribute to a more sustainable management of mine tailings deposits. While ureolytic activity has been the predominant driver of conventional MICP to date, the associated release of ammonia poses significant environmental hazards. This study explores a novel, environmentally friendly approach by replacing urea with sodium bicarbonate as the carbon source for MICP, with the aim of reducing urea requirements and thus ammonia release. Leveraging the principles of the MICP, it was observed that when urea concentration was reduced to 0.7 M, by replacing it with bicarbonate, surface strength was close to 400 kPa. Furthermore, wind tunnel testing showed a significant reduction in dust emission, where the mass loss rate of the biocemented sample was below 10 kg m⁻² h⁻¹, which means a 9-fold reduction compared to untreated samples. So, it was demonstrated that bicarbonate can effectively replace urea, achieving a relevant reduction of up to 75 % in urea use without compromising the surface strength of the biocemented tailings and reducing the environmental hazards of ammonia release. These results highlight the potential of bicarbonate-based MICP as an innovative and sustainable approach to mitigate the environmental impacts associated with tailings deposits.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104255"},"PeriodicalIF":6.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impacts of aeration modes and rates on nitrogen conversion and bacterial community composition in a 90-m3 silo composting reactor","authors":"Pengxiang Xu ,&nbsp;Shaoqi Xu ,&nbsp;Yuquan Wei ,&nbsp;Yongdi Liu ,&nbsp;Sheng Hang ,&nbsp;Yue Wang ,&nbsp;Longli Zhang ,&nbsp;Ji Li","doi":"10.1016/j.eti.2025.104265","DOIUrl":"10.1016/j.eti.2025.104265","url":null,"abstract":"<div><div>The silo composting reactor is applicable for the in-situ treatment of organic waste. Ensuring an adequate supply of oxygen can enhance the compost maturity. This study investigated the effects of aeration modes and rates on composting effectiveness and nitrogen conversion. The 24 h uninterrupted aeration mode (K60T0) was conductive to achieving an elevated temperature of 78 °C. The germination index value in the lower layer consistently exceeded 80 % with different aeration modes. The content of total nitrogen (excluding K60T0) and ammonia nitrogen increased progressively with the increase of aeration time. Meanwhile, nitrate nitrogen content exhibited fluctuations, with the lowest content observed in the middle layer. An aeration cycle of 50 min followed by a 10-min pause within an hour resulted in the highest amide nitrogen content of 5.93 g/kg and organic nitrogen content of 2 % in the upper layer. The dominant phylum and genera were <em>Firmicutes</em> and <em>Ureibacillus</em>, respectively. The relative abundance of <em>Firmicutes</em> initially decreased and then increased in general with prolonged aeration time. Additionally, the extension of aeration time altered the nitrogen cycling function within the reactor.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104265"},"PeriodicalIF":6.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenge or hindrance? The impact of artificial intelligence applications on corporate environmental responsibility under policy and public pressures","authors":"Weiwei Wu ,&nbsp;Dechao Ma","doi":"10.1016/j.eti.2025.104273","DOIUrl":"10.1016/j.eti.2025.104273","url":null,"abstract":"<div><div>Given increasing environmental degradation and the growing emphasis on sustainable development, corporate environmental responsibility (CER) has become a crucial business strategy for consumers, investors, and society. This study explores the relationship between artificial intelligence (AI) applications and CER, while considering the moderating effects of climate policy uncertainty and public environmental concern, using data on A-share listed companies in China from 2012 to 2020. The results reveal an inverted U-shaped relationship between AI applications and CER performance. Climate policy uncertainty weakens the positive impact of AI on CER, while public environmental concern strengthens it. The findings provide valuable guidance for firms on how to strategically apply AI to enhance CER performance, which is substantially important for firms to adapt to policy and public pressure, and achieve sustainable development.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104273"},"PeriodicalIF":6.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic impacts of anthropogenic and climatic drivers on total phosphorus dynamics in a mega river basin","authors":"Yuxin Hu ,&nbsp;Hai Su ,&nbsp;Huiyin Song ,&nbsp;Qian Xiong ,&nbsp;Zheng Zhou ,&nbsp;Bo Huang ,&nbsp;Xu Wang ,&nbsp;Naidong Xiao ,&nbsp;Mingxing Yu","doi":"10.1016/j.eti.2025.104259","DOIUrl":"10.1016/j.eti.2025.104259","url":null,"abstract":"<div><div>Total phosphorus (TP) pollution in the Yangtze River basin (YRB) severely threatens ecosystems. Previous studies have examined individual factors affecting TP, but the combined impacts of anthropogenic activities and climate factor remain unclear. This study analyzed TP data from 636 sites in the YRB during 2016–2020 using partial least squares structural equation modeling to assess these combined effects on TP pollution. Results showed government regulation significantly reduced TP concentration from 2016 to 2020. Anthropogenic activities, including changes in landscape, GDP, and population growth, significantly influenced TP concentrations. Cropland and impervious surfaces were major TP sources, while forests acted as effective TP sinks. Landscape fragmentation weakened the ability of source landscapes to export TP to water bodies and the purification capacity of sink landscapes, while aggregation enhanced sink capacity. GDP and population growth were positively correlated with TP, but their impacts were partially mitigated by landscape adjustments and policy interventions. Climate factor also significantly affected TP concentrations. Precipitation influenced surface runoff and pollutant generation, playing a key role in non-point source pollution and amplifying the positive impact of landscape sources on TP. Air temperature promoted the release of internal phosphorus by affecting water temperature, water body stratification, and dissolved oxygen concentrations. Wind speed could weaken the release of TP from landscape sources to water bodies. Future efforts should focus on optimizing landscape patterns, enhancing sink landscape aggregation, and strengthening environmental governance, while considering climate impacts in water quality management to ensure the sustainable development of the YRB.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104259"},"PeriodicalIF":6.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing rice productivity in wastewater-irrigated saline Cd-contaminated soils using microbial-nanoparticle synergy","authors":"Emad M. Hafez ,&nbsp;Yan Gao ,&nbsp;Honggui La ,&nbsp;Khadiga Alharbi ,&nbsp;Maha M. Hamada ,&nbsp;Alaa El-Dein Omara ,&nbsp;Tarek Alshaal","doi":"10.1016/j.eti.2025.104253","DOIUrl":"10.1016/j.eti.2025.104253","url":null,"abstract":"<div><div>Soil salinity and cadmium (Cd) contamination pose significant threats to agricultural productivity and food security, particularly in rice-growing regions. This study investigates the synergistic effects of plant growth-promoting rhizobacteria (PGPRs) (<em>Pseudomonas koreensis</em>, <em>Bacillus coagulans</em>, and <em>Pseudomonas stutzeri</em>) and selenium nanoparticles (SeNPs) in remediating saline Cd-contaminated soils and enhancing rice (<em>Oryza sativa</em> L.) performance. Over two consecutive growing seasons (2022–2023), the combined application of PGPRs and SeNPs significantly improved soil health, reducing soil pH from 8.50 to 8.02 and electrical conductivity (EC_e) from 5.97 to 4.01 dS m⁻¹ , while increasing soil organic matter (SOM) by 6.5 % and cation exchange capacity (CEC) by 25.6 %. The treatment also reduced soil Cd content by 34.6 %, from 0.81 to 0.53 mg kg⁻¹ , and decreased Cd accumulation in rice roots, shoots, and seeds by 56.7 %, 65.0 %, and 50.0 %, respectively, ensuring safer rice grain production. Furthermore, SeNPs significantly enhanced selenium (Se) content in rice shoots and seeds, with Se levels increasing from 0.55 to 1.47 µg g⁻¹ in shoots and from 0.01 to 0.51 µg g⁻¹ in seeds, highlighting their role in improving rice nutritional quality. Physiological analyses revealed enhanced photosynthetic pigment concentrations, with chlorophyll a increasing by 112.3 % and carotenoids by 213.6 %, alongside a 101.9 % increase in superoxide dismutase (SOD) activity under the combined treatment. These improvements translated into a 25.0 % increase in grain yield, from 5.76 to 7.24 ton ha⁻¹ , and a 21.4 % increase in 1000-grain weight. The findings highlight the efficacy of PGPRs and SeNPs in mitigating oxidative stress, improving nutrient uptake, reducing Cd toxicity, and enhancing rice productivity under combined salinity and Cd stress. This study provides a novel, eco-friendly strategy for sustainable soil remediation and crop production in contaminated environments, offering significant implications for global food security and agricultural sustainability.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104253"},"PeriodicalIF":6.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of temperature, pH and reaction time on alkali-thermal pretreatment of poly(3-hydroxybutyrate): Enhancing solubilization and anaerobic CH4 production","authors":"Minjae Kim ,&nbsp;Minsu Song ,&nbsp;Trang Thi Nhu Le ,&nbsp;Chaeyoung Rhee ,&nbsp;Mi Na Rhie ,&nbsp;Seung Gu Shin ,&nbsp;Joonyeob Lee","doi":"10.1016/j.eti.2025.104261","DOIUrl":"10.1016/j.eti.2025.104261","url":null,"abstract":"<div><div>The effects of alkali-thermal pretreatment with operational parameters (temperature: 37–91 °C, pH: 7–13, pretreatment time: 24–120 hr) on the solubilization and the consecutive anaerobic digestion of poly(3-hydroxybutyrate), P3HB, was investigated using the tests with 3²×4 full factorial design. Near-complete solubilization of P3HB (99.6 %) was attained through the alkali-thermal pretreatment. Alkali-thermal pretreatment of P3HB significantly improved CH₄ production, resulting in 23-fold enhancement in the CH₄ production rate (73 mL CH₄/g VSS/d) and an 88 % reduction in elapsed batch time for complete anaerobic digestion, with a CH₄ yield of 380 mL CH₄/g COD. Pretreatment factors, particularly pH, exerted significant effects and showed synergistic effects with temperature. This study integrated factorial design, response surface modeling, and microbial analysis to offer a comprehensive understanding of how pretreatment conditions affected both performance and microbial dynamics in P3HB anaerobic digestion. Response surface analysis with partial cubic models successfully quantified these relationships, and strong positive correlations between solubilization and CH₄ yield were observed. Distinct bacteria and archaea communities proliferated during the anaerobic digestion of pretreated versus unpretreated P3HB. Spirochaetaceae, <em>Syntrophomonas</em>, <em>Mesotoga</em>, Christensenellaceae R-7 group, and <em>Sedimentibacter</em>, along with methanogens such as <em>Methanosaeta</em> and <em>candidatus Methanofastidiosum</em>, were identified as key contributors to the anaerobic digestion of P3HB. These findings support that optimized alkali-thermal pretreatment significantly enhances the anaerobic digestion of P3HB, underscoring the potential of P3HB wastes as prospective feedstocks for anaerobic digestion process.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"39 ","pages":"Article 104261"},"PeriodicalIF":6.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}