Environmental Technology & Innovation最新文献

{"title":"DsbA-L deficiency promotes cigarette smoke-induced bronchial epithelial cells ferroptosis by inhibiting catalase in COPD","authors":"Siqi Li ,&nbsp;Zhenyu Peng ,&nbsp;Qiong Huang ,&nbsp;Qiong Chen ,&nbsp;Baimei He","doi":"10.1016/j.eti.2024.103923","DOIUrl":"10.1016/j.eti.2024.103923","url":null,"abstract":"<div><h3>Background</h3><div>Ferroptosis, characterized by iron-dependent programmed cell death, has been implicated in chronic obstructive pulmonary disease (COPD). Recent studies have shown that the disulfide-bond A oxidoreductase-like protein (DsbA-L) is associated with various diseases. However, the involvement of DsbA-L in COPD remains unclear.</div></div><div><h3>Methods</h3><div>To establish a COPD model, 8-week-old male mice were exposed to cigarette smoke (CS) for 6 months. BEAS-2B cells were cultured with cigarette smoke extract (CSE) in vitro. DsbA-L siRNA, DsbA-L plasmid, or catalase siRNA were used to elucidate the underlying mechanisms. Lung function; lung histopathology; Fe²⁺ concentration; glutathione (GSH), reactive oxygen species (ROS), 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA) levels; and protein expression of DsbA-L, xCT, glutathione peroxidase-4 (GPX4), and catalase were measured.</div></div><div><h3>Results</h3><div>DsbA-L expression was significantly decreased in the lung tissues of COPD mice and CSE-treated BEAS-2B cells. DsbA-L knockout exacerbated COPD progression by increasing ferroptosis, as confirmed by reduced GSH, xCT, and GPX4 levels and elevated Fe²⁺, ROS, 4-HNE and MDA levels. Catalase expression was also attenuated in the lung tissues of COPD mice and CSE-treated BEAS-2B cells. DsbA-L overexpression ameliorated ferroptosis by upregulating catalase expression in BEAS-2B cells, whereas catalase knockdown abolished the effects of DsbA-L overexpression on ferroptosis.</div></div><div><h3>Conclusion</h3><div>DsbA-L deficiency exacerbated COPD progression by promoting ferroptosis in bronchial epithelial cells through catalase inhibition. These findings indicate that DsbA-L may be an underlying therapeutic strategy for COPD.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103923"},"PeriodicalIF":6.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing efficacy of AgCuFe2O4@GO/MnO2 in 3D electrochemical oxidation for ceftriaxone degradation in aqueous media: Optimization and mechanisms","authors":"Majid Hashemi ,&nbsp;Fatemeh Rahimi ,&nbsp;Sahar Abolghasemi ,&nbsp;Alireza Nasiri ,&nbsp;Saeed Rajabi","doi":"10.1016/j.eti.2024.103914","DOIUrl":"10.1016/j.eti.2024.103914","url":null,"abstract":"<div><div>Ceftriaxone (CEF), a broad-spectrum antibiotic with a long half-life, is extensively used for the curative purposes of many bacterial infections. Nevertheless, given its strong resistance and the inefficiency of traditional techniques in breaking it down and eliminating it, this study assessed the use of a synthesized AgCuFe₂O₄@GO/MnO₂ nanoparticle electrode (NPE) in a three-dimensional electrochemical oxidation reaction (3DER) to remove CEF. The AgCuFe₂O₄@GO/MnO₂ NPE was fabricated by a co-precipitation process aided by a microwave. The physical and chemical structure of the nanocomposite was determined and verified using a range of analytical techniques, such as FESEM, XRD, EDS-mapping, FTIR, BET, VSM, and TGA. These investigations indicated that the NPE had a large specific surface area, a maintained crystal structure, strong magnetic characteristics, and a quasi-spherical morphology. A 3DER with optimal parameters (pH 5, initial CEF concentration 20 mg/L, NPE dose 0.7 g/L, electrode distance 3 cm, 0.12 mM persulfate electrolyte, and 8.5 mA/cm² current density for 45 minutes) removed 86.8 % of CEF in synthetic samples and 71.3 % in real wastewater samples, with a mineralization rate of 53.4 %, also had 253.2 kWh/g energy consumption. The 3DER matched the <em>pseudo</em>-first-order kinetic and the Langmuir-Hinshelwood model (R² &gt; 0.9), with <em>K</em>_<em>C</em> and <em>K</em>_<em>L-H</em> values of 0.954 mg/L.min and 0.032 L/mg. The removal effectiveness of 64.9 % was achieved after five cycles of recovering and regenerating the NPE. The AgCuFe₂O₄@GO/MnO₂ NPE is beneficial for treating a wide range of industrial and hospital wastewaters due to its magnetic characteristics, chemical stability, reusability, and remarkable efficiency.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103914"},"PeriodicalIF":6.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive effects of nitrogen fertilization on the flavor ingredients of tea (Wuniuzao), soil physicochemical properties, and microbial communities","authors":"Rongxiu Yin ,&nbsp;Lulu Li ,&nbsp;Xin Li ,&nbsp;Huifang Liu ,&nbsp;Jianmei Yao ,&nbsp;Chiyu Ma ,&nbsp;Lulu Pu ,&nbsp;Yutao Peng ,&nbsp;Zhiwei Lei","doi":"10.1016/j.eti.2024.103911","DOIUrl":"10.1016/j.eti.2024.103911","url":null,"abstract":"<div><div>Nitrogen fertilizer is crucial in tea plantation management, but its effects on tea quality, soil environment, and microbial communities under different fertilization schemes remain unclear. This study evaluated the impacts of six nitrogen application levels on tea quality, soil physicochemical properties, and microbial community structures, aiming to reveal the optimization effects of nitrogen application on the soil environment and its significance for sustainable agriculture. Results showed that nitrogen application (especially under N3 and N4 treatments) increased total free amino acid (TA) content, total catechin content, and caffeine content in tea leaves, but reduced the TP/TA ratio. Significant changes in soil physicochemical properties were observed, including a decrease in pH, an increase in electrical conductivity (EC), higher levels of available potassium (Ava K) and available phosphorus (Ava P), an increase in soil organic carbon (SOC) content, and a reduction in available nitrogen (Ava N) content. These changes indicate that nitrogen application optimized soil nutrient status to some extent but may also contribute to environmental issues such as soil acidification. Regarding soil microbial communities, higher nitrogen application led to an increase in beneficial bacteria abundance, a more complex microbial community structure, and tighter interaction networks. Mantal Test analysis indicated that the changes in tea leaf biochemical components under nitrogen fertilization were the result of the combined effects of soil physicochemical properties and key microbial communities. Furthermore, soil bacterial characteristics were more strongly correlated with changes in tea quality. Based on random forest model analysis, <em>p__Proteobacteria</em> (associated with total catechin and caffeine) and <em>p__Firmicutes</em> (associated with TP/TA) were the key microbial communities influencing tea flavor components, while soil EC (affecting total catechin and TP/TA) and SOC content (affecting caffeine) were the key soil physicochemical properties influencing tea flavor components. Overall, nitrogen application not only improved tea quality but also significantly impacted soil environment and microbial community structure, indicating that appropriate nitrogen application (194.58-223.61 kg N ha⁻¹) plays a vital role in enhancing tea quality and promoting sustainable management of soil ecosystems.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103911"},"PeriodicalIF":6.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyacrylonitrile fiber supported nano zero-valent iron activated persulfate to degrade organophosphorus and simultaneously adsorb the produced phosphate","authors":"Shengjin Tan ,&nbsp;Shangyuan Zhao ,&nbsp;Panpan Zhang ,&nbsp;Peisen Liu ,&nbsp;Qizhong Xiong ,&nbsp;Chaochun Zhang ,&nbsp;Gang Xu ,&nbsp;Xian-Lei Shi ,&nbsp;Yusef Kianpoor Kalkhajeh ,&nbsp;Xinxin Ye","doi":"10.1016/j.eti.2024.103912","DOIUrl":"10.1016/j.eti.2024.103912","url":null,"abstract":"<div><div>Degradation of organic phosphorus in water has received widespread attention. However, the synchronous recovery of generated phosphate remains a challenge. Herein, a novel nano zero-valent iron (nZVI) supported polyacrylonitrile fiber (PAN_AF-Fe⁰) was constructed to activate persulfate (PDS) for simultaneously degradation of organic P and removal of the generated phosphate. The results revealed that nZVI loading significantly increased the activation ability of PDS to degrade phenyl phosphonic acid (PPOA) with degradation rate over 99 % in 2 h, the SO₄^−• and •OH generated in the PAN_AF-Fe⁰/PDS system had major contributions. Furthermore, PAN_AF-Fe⁰ showed a high capacity of 5.12 mg P g⁻¹ for the removal of generated phosphate mainly via the formation of Fe-P complexes on the fiber surface. Besides, the PAN_AF-Fe⁰ possesses advantages of wide pH application range, remarkable anti-interference ability and excellent reusability, which provides an innovative technology of universal significance for the recovery of aquatic organic P.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103912"},"PeriodicalIF":6.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing environmental impacts of various solvent-dissolution routes for the pelletization of mixed plastic waste","authors":"Junaid Saleem ,&nbsp;Zubair Khalid Baig Moghal ,&nbsp;Furqan Tahir ,&nbsp;Tareq Al-Ansari ,&nbsp;Gordon McKay","doi":"10.1016/j.eti.2024.103903","DOIUrl":"10.1016/j.eti.2024.103903","url":null,"abstract":"<div><div>The vast majority of mixed plastic waste (MPW) does not degrade, thereby contributing to permanent environmental pollution. To address this, solvent-dissolution processes have been developed to recycle MPW into usable materials like blended pellets. This study evaluates five solvent-dissolution pathways, with a focus on mixed polyolefin, which constitutes about 60 % of MPW. The pathways explored include xylene, paraffin wax<img>heptane, paraffin wax<img>hexane, mineral oil<img>heptane, and mineral oil<img>hexane. Although paraffin wax and mineral oil are considered environmentally friendly, their recovery processes involve hexane or heptane, leading to higher energy use and carbon emissions. The xylene-based pathway, however, showed about four times lower energy consumption (48 MJ) and significantly reduced emissions (1.24 kg CO2 eq.) compared to fossil fuel-derived methods. In addition, mechanical property tests were conducted on the HD-PP blends which revealed a tensile modulus of 762 MPa and a tensile strength of 24 MPa, rendering them potentially advantageous for applications necessitating moderate elasticity and strength. Additionally, the study identifies dissolution and extrusion as the key stages affecting the life cycle assessment (LCA) and suggests powder production as a more cost-effective and sustainable alternative to pelletization, with lower carbon emissions. These findings offer valuable insights for improving MPW recycling through solvent-dissolution.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103903"},"PeriodicalIF":6.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ammonium transporter AmtB is dispensable for the uptake of ammonium in the phototrophic diazotroph Rhodopseudomonas palustris","authors":"Lu Huang ,&nbsp;Weilai Lu ,&nbsp;Yunkai Yu ,&nbsp;Haoning Qiu ,&nbsp;Yan Zeng ,&nbsp;Lida Wang ,&nbsp;Ying Liu ,&nbsp;Lei Yan ,&nbsp;Yu Vincent Fu ,&nbsp;Yanning Zheng","doi":"10.1016/j.eti.2024.103853","DOIUrl":"10.1016/j.eti.2024.103853","url":null,"abstract":"<div><div>Ammonium (NH₄⁺) transport across cell membranes plays an important role in assimilation or removal of the environmental nitrogen. The membrane protein AmtB has been considered to be the NH₄⁺ transporter that is responsible for the NH₄⁺ uptake. The phototrophic diazotroph <em>Rhodopseudomonas palustris</em> harboring two <em>amtB</em> genes has been widely used in wastewater treatment and bioremediation. However, the role of AmtB in NH₄⁺ uptake remains unclear in <em>R. palustris</em>. Here, we employed an innovative approach combining stable isotope probing (SIP) with Raman spectroscopy to determine the physiological functions of AmtB1 and AmtB2 in <em>R. palustris.</em> This powerful technique allowed us to investigate NH₄⁺ uptake at the single-cell level. The generated <em>R. palustris</em> Δ<em>amtB1</em> Δ<em>amtB2</em> mutant lacking AmtB1 and AmtB2 proteins was still capable of utilizing ¹⁵NH₄⁺ even the ¹⁵NH₄⁺ concentration was as low as 5 μM. These data demonstrate that both of the AmtB proteins are not essential for <em>R. palustris</em> to take up NH₄⁺ regardless of environmental NH₄⁺ levels. However, both AmtB1 and AmtB2 can contribute to NH₄⁺ uptake under nitrogen-limiting conditions. Given that <em>R. palustris</em> primarily expresses AmtB2 in these conditions, AmtB2 plays a more important role in NH₄⁺ uptake compared to AmtB1. In addition, transcriptomic analysis showed that the deletion of the <em>amtB1</em> and <em>amtB2</em> genes resulted in the upregulation of many transporter genes, providing potential targets for future investigation of alternative NH₄⁺ uptake systems.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"36 ","pages":"Article 103853"},"PeriodicalIF":6.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An innovative sustainable solution: Recycling shield-discharge waste soil as fine aggregate to produce eco-friendly geopolymer-based flowable backfill materials","authors":"Guijie Zhao ,&nbsp;Hongzhan Guan ,&nbsp;Huan Yan ,&nbsp;Yunkai Ruan ,&nbsp;Yafei Han ,&nbsp;Xiaoqiang Pan ,&nbsp;Jinfeng Tian ,&nbsp;Bo Liu","doi":"10.1016/j.eti.2024.103857","DOIUrl":"10.1016/j.eti.2024.103857","url":null,"abstract":"<div><div>The disposal of Shield-Discharge Waste Soil (SDWS) is substantial, yet their recycling rate remains low, necessitating the exploration of new recycling methods for effective waste management. This study examines the potential use of SDWS as a fine aggregate in the production of Controlled Low-Strength Material (CLSM), incorporating slag and fly ash as precursors. Key properties such as strength, flowability, setting time, microstructure, chemical composition, CO₂ emissions, energy consumption, and cost were assessed and analyzed. Additionally, the environmental impact and material-related strategies were discussed. The results reveal that the developed CLSM exhibits competitive performance, with compressive strengths ranging from 2.830 MPa to 4.121 MPa, achieving over 1.0 MPa strength within 24 hours. The material demonstrates high flowability, exceeding 200 mm within 30 minutes, and has a setting time between 2.10 and 4.23 hours, offering advantages in both setting time and early strength. SDWS contributes to extending the coagulation process and enhancing the flowability. Optimal strength is observed when SDWS constitutes approximately 30 % of the binders or when the alkali equivalent is around 7 %. Compared to traditional cement-based CLSMs, incorporating SDWS results in reduced CO₂ emissions and lower energy consumption. When considering savings from reduced waste disposal costs, the overall material cost remains competitive. Furthermore, higher SDWS content leads to enhanced environmental benefits, and it is recommended to keep the alkali equivalent below 7 % for optimal performance. In conclusion, the developed CLSM presents significant potential for wide-scale applications and offers a sustainable solution for recycling SDWS.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"36 ","pages":"Article 103857"},"PeriodicalIF":6.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remediation of Pb and Cd contaminated sediments by wheat straw biochar and microbial community analysis","authors":"Yanhao Zhang ,&nbsp;Wenlu Zhou ,&nbsp;Chengying Liu ,&nbsp;Guanghui Liu ,&nbsp;Yi Li ,&nbsp;Botao Ding ,&nbsp;Peng Zhang ,&nbsp;Xu Zhang ,&nbsp;Zhibin Zhang","doi":"10.1016/j.eti.2024.103849","DOIUrl":"10.1016/j.eti.2024.103849","url":null,"abstract":"<div><div>Wheat straw biochar (BC) or modified wheat straw biochar (MBC) could affect the aggregate structure of soil, effectively adsorb heavy metal and reduce the bioavailability of heavy metals in soil. TGA, BET, FTIR and SEM were used to characterize the surface morphology and structure of BC and MBC. The adsorption properties of BC and MBC on Pb(‌II) and Cd(‌II) in solution systems were investigated by batch experiments, and the remediation performance of Pb and Cd contaminated sediment by BC or MBC were studied by pot experiments. The results showed that MBC had a larger specific surface area of 46.04 m²/g and total pore volume 0.0555 cm³/g; the adsorption isotherms of Pb(II) and Cd(II) for BC and MBC were more suitable for Langmuir model (<em>R</em>^<em>2</em> over 0.91), and the maximum adsorption capacities of Pb(II) and Cd(II) by MBC could reach 71.14 and 115.64 mg/g, respectively. BC or MBC promoted the transformation of Pb and Cd from the extractable acid fraction to the oxidizable and residual fraction, reduced the migration of heavy metals and enhanced the stability of aggregates. Therefore, the results provided a perspective for the dredged contaminated sediments used as planting soil. In addition, BC or MBC, as soil amendments, could improve the abundance of microbial community. At the phylum level, the main dominant bacteria were <em>Proteobacteria</em> and <em>Bacteroidetes</em>.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"36 ","pages":"Article 103849"},"PeriodicalIF":6.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing subgroup differences and underlying causes of ozone-associated mortality burden in China using multi-source data","authors":"Yong Li ,&nbsp;Maofei Ni ,&nbsp;Qingying Liu ,&nbsp;Qin Liao ,&nbsp;Yun Bai ,&nbsp;Chuan Li","doi":"10.1016/j.eti.2024.103856","DOIUrl":"10.1016/j.eti.2024.103856","url":null,"abstract":"<div><div>Long-term ozone exposure is a significant public health concern. To mitigate its impact, it is crucial to identify diseases, populations, and regions that are particularly sensitive to ozone. In this study, we used high-quality data and updated relative risk estimates to investigate subgroup differences in mortality burden due to ozone exposure in China. In 2020, nationwide ozone-associated deaths totaled 276,800 (95 % CI: 106,700–451,900), representing 3.2 % of all deaths, with respiratory and cardiovascular diseases accounting for 16.8 % and 83.2 %, respectively. The per capita ozone-associated mortality in China was 20 per 100,000 person-years, with a significantly higher rate observed in elderly individuals aged ≥75 years (189 deaths per 100,000) compared to young individuals aged 30–44 years (1 death per 100,000). There were notable spatial patterns of ozone-associated deaths among the regions, indicating a critical response to ozone levels and population density. Interestingly, urban areas exhibited a 22.4 % lower mortality rate related to ozone compared to rural areas. This difference was attributed to the combined influence of ozone exposure (+19.9 %), population size (+17.7 %), age structure (−39.2 %), and baseline mortality (−20.8 %). By emphasizing subgroup differences and driving factors, these findings significantly enhance the understanding of the ozone-associated mortality burden, providing valuable insights for policymakers.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"36 ","pages":"Article 103856"},"PeriodicalIF":6.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchronously improving intracellular electron transfer in electron-donating bacteria and electron-accepting methanogens for facilitating direct interspecies electron transfer during anaerobic digestion of kitchen wastes","authors":"Xin He ,&nbsp;Zhipeng Ao ,&nbsp;Yuan Li ,&nbsp;Yang Li ,&nbsp;Zhiqiang Zhao ,&nbsp;Yaobin Zhang","doi":"10.1016/j.eti.2024.103843","DOIUrl":"10.1016/j.eti.2024.103843","url":null,"abstract":"<div><div>Two major issues generally limit the effectiveness of direct interspecies electron transfer (DIET) during anaerobic digestion: 1) lack of essential electrical connection component, electrically conductive pili (e-pili) or multi-heme <em>c</em>-type cytochrome (MHC); 2) the thermodynamic limitations of combining electrons with protons for reducing carbon dioxide to methane. To address both issues, a strategy for facilitating DIET via combining ethanol-type fermentation pretreatment (EFP) with NaCl addition during anaerobic digestion of kitchen wastes was proposed. Combining EFP with NaCl addition dramatically increased methane yield rates (531.3 ± 11.4 vs 410.5 ± 8.7 mL/gVS_added/d) and efficiencies of conversion of organic substrates to methane (571.2 ± 13.8 vs 488.7 ± 11.7 mL/gVS_removal). The increased performances by combining EFP with NaCl addition were higher than that by independent EFP or NaCl addition. <em>Paludibacter sp.</em>, <em>Petrimonas sp.</em> and <em>Syntrophomonas wolfei</em> were the predominant and metabolically active electron-donating bacteria, and their expression of genes for e-pili/MHCs by combining EFP with NaCl addition was higher than that by independent EFP or NaCl addition. <em>Methanothrix soehngenii</em> and <em>Methanoculleus bourgensis</em> were the predominant and metabolically active electron-accepting methanogens, and their expression of genes for carbon dioxide reduction pathway for methanogenesis by combining EFP with NaCl addition was higher than that by independent EFP or NaCl addition. In addition, EFP specifically increased the transcript abundance of genes for NAD⁺/NADH transformation closely associated with the formation of e-pili/MHCs, while NaCl addition specifically increased the transcript abundance of genes for coenzyme F₄₂₀/F₄₂₀H₂ transformation known to participate in reduction of carbon dioxide to methane.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"36 ","pages":"Article 103843"},"PeriodicalIF":6.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}