Mohamed A. Fahmy , Sally Attia , Maha M. Nader , Sarah I.Z. Abdel-Wahab , Muhammad Ayman , Layla A. Almutairi , Mohammed A. Alqahtani , Naheda M. Alshammari , Nada M. Abd El-Moaty , Mohamed Maher , Mahmoud Sitohy , Mohamed T. El-Saadony
{"title":"一种新型菌群对氯地那福-丙炔的分子特性和土壤生物修复作用","authors":"Mohamed A. Fahmy , Sally Attia , Maha M. Nader , Sarah I.Z. Abdel-Wahab , Muhammad Ayman , Layla A. Almutairi , Mohammed A. Alqahtani , Naheda M. Alshammari , Nada M. Abd El-Moaty , Mohamed Maher , Mahmoud Sitohy , Mohamed T. El-Saadony","doi":"10.1016/j.eti.2025.104366","DOIUrl":null,"url":null,"abstract":"<div><div>Clodinafop-propargyl (CF) is a widely used herbicide with high persistence in soil, raising concerns about its long-term environmental impact. Bacterial biodegradation provides a sustainable and eco-friendly strategy to mitigate CF contamination. This study evaluated the in-situ bioremediation potential of a six-member bacterial consortium (T3) by measuring CO₂ evolution as an indicator of microbial activity and monitoring CF residues in two soil types using microcosm systems. The consortium included <em>Bacillus subtilis</em> subsp. <em>subtilis</em> AZFS3 (LC599401.1), <em>Bacillus pumilus</em> AZFS5 (LC599402.1), <em>Bacillus mojavensis</em> AZFS15 (LC599403.1), <em>Bacillus paramycoides</em> AZFS18 (LC599406.1), <em>Pseudomonas aeruginosa</em> KZFS4 (LC599404.1), and <em>Alcaligenes aquatilis</em> KZFS11 (LC599405.1). T3 performance was compared to two individual strains representing Gram-positive and Gram-negative bacteria: <em>B. subtilis</em> subsp. <em>subtilis</em> AZFS3 (T1) and <em>P. aeruginosa</em> KZFS4 (T2). T3 achieved the highest degradation efficiency among all treatments, with CO₂ emissions nearing zero by day 20 (0.120 mg CO₂/100 g/DW. soil, equivalent to 0.63 % CF residue in sandy loam). CF residues were significantly reduced to 0.34 % (0.16 mg/kg sandy loam soil) and 6.38 % (2.99 mg/kg clay soil), underscoring the consortium’s strong bioremediation capacity, as determined by HPLC. Metabolite profiling, gene expression analysis, and pathway prediction further supported the consortium’s superiority over individual strains. The enzymes required for CF degradation were distributed across all six bacterial strains. The molecular characterization of the CF degradation pathway, using integrated bioinformatic and experimental approaches, confirmed its collective encoding by the individual members of the bacterial hexa-consortium. The Maleylacetate reductase (EC 1.3.1.32), crucial for the complete CF breakdown, was found exclusively in <em>P. aeruginosa</em>, suggesting a potential enzymatic bottleneck and a limiting factor. Future research should focus on enriching the consortium with additional strains expressing this enzyme to provide other alternatives capable of enhancing the degradation efficiency. The six-strain consortium offers a practical and environmentally sound solution for remediating CF-contaminated soils.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"40 ","pages":"Article 104366"},"PeriodicalIF":7.1000,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization and soil bioremediation of Clodinafop-propargyl by a novel bacterial consortium\",\"authors\":\"Mohamed A. Fahmy , Sally Attia , Maha M. Nader , Sarah I.Z. Abdel-Wahab , Muhammad Ayman , Layla A. Almutairi , Mohammed A. Alqahtani , Naheda M. Alshammari , Nada M. Abd El-Moaty , Mohamed Maher , Mahmoud Sitohy , Mohamed T. El-Saadony\",\"doi\":\"10.1016/j.eti.2025.104366\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Clodinafop-propargyl (CF) is a widely used herbicide with high persistence in soil, raising concerns about its long-term environmental impact. Bacterial biodegradation provides a sustainable and eco-friendly strategy to mitigate CF contamination. This study evaluated the in-situ bioremediation potential of a six-member bacterial consortium (T3) by measuring CO₂ evolution as an indicator of microbial activity and monitoring CF residues in two soil types using microcosm systems. The consortium included <em>Bacillus subtilis</em> subsp. <em>subtilis</em> AZFS3 (LC599401.1), <em>Bacillus pumilus</em> AZFS5 (LC599402.1), <em>Bacillus mojavensis</em> AZFS15 (LC599403.1), <em>Bacillus paramycoides</em> AZFS18 (LC599406.1), <em>Pseudomonas aeruginosa</em> KZFS4 (LC599404.1), and <em>Alcaligenes aquatilis</em> KZFS11 (LC599405.1). T3 performance was compared to two individual strains representing Gram-positive and Gram-negative bacteria: <em>B. subtilis</em> subsp. <em>subtilis</em> AZFS3 (T1) and <em>P. aeruginosa</em> KZFS4 (T2). T3 achieved the highest degradation efficiency among all treatments, with CO₂ emissions nearing zero by day 20 (0.120 mg CO₂/100 g/DW. soil, equivalent to 0.63 % CF residue in sandy loam). CF residues were significantly reduced to 0.34 % (0.16 mg/kg sandy loam soil) and 6.38 % (2.99 mg/kg clay soil), underscoring the consortium’s strong bioremediation capacity, as determined by HPLC. Metabolite profiling, gene expression analysis, and pathway prediction further supported the consortium’s superiority over individual strains. The enzymes required for CF degradation were distributed across all six bacterial strains. The molecular characterization of the CF degradation pathway, using integrated bioinformatic and experimental approaches, confirmed its collective encoding by the individual members of the bacterial hexa-consortium. The Maleylacetate reductase (EC 1.3.1.32), crucial for the complete CF breakdown, was found exclusively in <em>P. aeruginosa</em>, suggesting a potential enzymatic bottleneck and a limiting factor. Future research should focus on enriching the consortium with additional strains expressing this enzyme to provide other alternatives capable of enhancing the degradation efficiency. 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Molecular characterization and soil bioremediation of Clodinafop-propargyl by a novel bacterial consortium
Clodinafop-propargyl (CF) is a widely used herbicide with high persistence in soil, raising concerns about its long-term environmental impact. Bacterial biodegradation provides a sustainable and eco-friendly strategy to mitigate CF contamination. This study evaluated the in-situ bioremediation potential of a six-member bacterial consortium (T3) by measuring CO₂ evolution as an indicator of microbial activity and monitoring CF residues in two soil types using microcosm systems. The consortium included Bacillus subtilis subsp. subtilis AZFS3 (LC599401.1), Bacillus pumilus AZFS5 (LC599402.1), Bacillus mojavensis AZFS15 (LC599403.1), Bacillus paramycoides AZFS18 (LC599406.1), Pseudomonas aeruginosa KZFS4 (LC599404.1), and Alcaligenes aquatilis KZFS11 (LC599405.1). T3 performance was compared to two individual strains representing Gram-positive and Gram-negative bacteria: B. subtilis subsp. subtilis AZFS3 (T1) and P. aeruginosa KZFS4 (T2). T3 achieved the highest degradation efficiency among all treatments, with CO₂ emissions nearing zero by day 20 (0.120 mg CO₂/100 g/DW. soil, equivalent to 0.63 % CF residue in sandy loam). CF residues were significantly reduced to 0.34 % (0.16 mg/kg sandy loam soil) and 6.38 % (2.99 mg/kg clay soil), underscoring the consortium’s strong bioremediation capacity, as determined by HPLC. Metabolite profiling, gene expression analysis, and pathway prediction further supported the consortium’s superiority over individual strains. The enzymes required for CF degradation were distributed across all six bacterial strains. The molecular characterization of the CF degradation pathway, using integrated bioinformatic and experimental approaches, confirmed its collective encoding by the individual members of the bacterial hexa-consortium. The Maleylacetate reductase (EC 1.3.1.32), crucial for the complete CF breakdown, was found exclusively in P. aeruginosa, suggesting a potential enzymatic bottleneck and a limiting factor. Future research should focus on enriching the consortium with additional strains expressing this enzyme to provide other alternatives capable of enhancing the degradation efficiency. The six-strain consortium offers a practical and environmentally sound solution for remediating CF-contaminated soils.
期刊介绍:
Environmental Technology & Innovation adopts a challenge-oriented approach to solutions by integrating natural sciences to promote a sustainable future. The journal aims to foster the creation and development of innovative products, technologies, and ideas that enhance the environment, with impacts across soil, air, water, and food in rural and urban areas.
As a platform for disseminating scientific evidence for environmental protection and sustainable development, the journal emphasizes fundamental science, methodologies, tools, techniques, and policy considerations. It emphasizes the importance of science and technology in environmental benefits, including smarter, cleaner technologies for environmental protection, more efficient resource processing methods, and the evidence supporting their effectiveness.