E-Journal Menara Perkebunan最新文献

{"title":"Waste reduction and nutrient recovery during the co-composting of empty fruit bunches and palm oil mill effluent","authors":"V. Baron, Jajang Supriatna, Clarisse Maréchal, Rajiv Sadasiban, X. Bonneau","doi":"10.22302/iribb.jur.mp.v87i2.338","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v87i2.338","url":null,"abstract":"AbstrakMinyak kelapa sawit adalah minyak nabati yang paling banyak dikonsumsi dunia. Setengah dari produksinya berasal dari Indonesia, walaupun perluasannya telah dikritik dari sudut pandang lingkungan. Pengurangan dampak lingkungan perkebunan melalui praktik pengelolaan limbah yang lebih baik sangat penting untuk mencapai produksi yang lebih bersih. Dalam konteks ini, penelitian difokuskan pada pengomposan, praktik yang semakin banyak diterapkan di agroindustri. Penelitian bertujuan untuk menguji pengomposan produk samping pabrik kelapa sawit yaitu tandan kosong kelapa sawit (TKKS) dan limbah cair pabrik kelapa sawit (LCPKS), pada rasio LCPKS/TKKSdan frekuensi pembalikan yang berbeda. Setelah 60 hari, kompos masih dalam fase mesofilik dan tidak dapat dianggap sebagai kompos matang karena rasio C/N dan suhu yang tinggi. Penurunan bobot dan volume yang tinggi telah dicapai masing-masing sebesar 40% dan 60%, serta penguapan air yang signifikan dari LCPKSdan TKKS(60%). Rasio LCPKSterhadap TKKSpada 1 – 1.5 m3/ton adalah optimal untuk mencapai kelembaban (65-70%), ruang udara bebas (>50%) dan pemulihan nutrisi, juga menunjukkan bahwa dalam kondisi percobaan ini proses pengomposan tidak dapat menggunakan semua LCPKSyang diproduksi oleh pabrik (3m3/ton TKKS). Tingkat pemulihan nutrisi mendekati 100% untuk fosfor, kalium dan magnesium, sedangkan untuk nitrogen terjadikehilangan sekitar 30-35%. Pengomposan dengan platform beton dan beratap, tidak melakukan penyemprotan pada tumpukan secara berlebihan, dan mendaur ulang semua limbah cair merupakan hal penting untuk mencapai efisiensi pemulihan nutrisi yang tinggi dan untuk mengontrol kualitas kompos akhir.[Kata kunci:pengomposan, tandan kosong, pemulihan nutrisi, kelapa sawit, limbah cair pabrik kelapa sawit, keberlanjutan]AbstractPalm oil is the most consumed edible oil in the world. Roughly half of the production originates from Indonesia, where the expansion of the crop has been criticized from an environmental perspective. Reducing the environmental impact of plantations through better waste management practices is critical to achieve cleaner production. In this context, our study was focused on composting, a practice increasingly adopted among agro-industries. Our trial was designed to test co-composting of the main palm oil mill by-products – empty fruit bunches (EFB) and palm oil mill effluent (POME) – under different POME/EFB ratios and turning frequencies. After 60 days the compost was still in a mesophilic phase and could not be considered as mature compost due to high C/N ratio and temperature. High weight and volume reduction were achieved (40% and 60% respectively), as well as significant water evaporation from the POME and EFB (60%). We found that a POME to EFB ratio of 1 to 1.5 m3/ton was optimal for moisture (65-70%), free air space (>50%) and nutrient recovery, showing that in our experimental conditions the composting process could not use all the POME produced by the mill (3m3/ton of EFB","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74777041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aktivitas amilase bakteri amilolitik asal larva black soldier fly (Hermetia illucens)","authors":"Irma Kresnawaty, Rizki Wahyu, Ashadi Sasongko","doi":"10.22302/iribb.jur.mp.v87i2.341","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v87i2.341","url":null,"abstract":"AbstractAmylase is an enzyme that has been widely used as a biocatalyst in foodand bioethanol industries. The availability of thermostable amylase will further expand the market and extend the shelf life of this enzyme. Amylase is produced by amylolytic bacteria using media with high-costnitrogen sources, such as pepton. Black soldier fly (BSF) is a potential source of amylolitic bacteria since its ability to degrade organic matters rapidly. This research aimedtoexploreamylolitic bacteria from the larvae of BSF with highest amylase activity that can be produced using low-cost media. The screening ofamylase activity was conducted by culturing the bacteria on starch containing media.Bacteria with the highest amylase activity were cultured in liquid media with twodifferentnitrogensources (urea and nitrate). Determinations of the optimum pH and temperature for this enzyme activity were carried out in the pH range 4to 7 and temperature 35to 65 ºC. Three amylase-producing isolates were obtained in this study. M1 isolate which has the highest activity was characterized based oncatalase activity and Gram staining. The results showed that the M1 isolate mightbelong togenus Proteussp. At the optimum condition (45ºC and pH 7), amylase activityin nitrate mediawas0.791U/mL, which was about 18-folds higher than that in ureamedia (0,041U/mL). Thus, amylase isolated from BSF larvae can be classified as a mesophilic enzyme and has the potential to be developed commercially at lower production costs.[Keywords:crude extract enzyme,Proteus sp.,thermostable] AbstrakAmilase merupakan salah satu enzim yangtelah digunakan secara luas sebagai biokatalis dalam industri pangan dan bioetanol.Ketersediaan amilase termostabil akan semakin memperluas pasar dan memperpanjang daya simpan enzim ini. Selama ini, produksi amilase dilakukan dengan memanfaatkan bakteri amilolitik menggunakan media dengan sumber nitrogen yang mahal, misalnya pepton. Black soldier fly (BSF) merupakan sumber bakteri amilolitik yang potential karena BSF memiliki kemampuan mendegradasi bahan organik dengan cepat.  Penelitian ini bertujuan untuk mengeksplorasi bakteri amilolitik dengan kemampuan amilase tinggi yang dapat diproduksi menggunakan media yanglebihmurah.Skrining bakteri penghasil amilase dilakukan dengan menumbuhkan bakteri pada media yang mengandung pati. Bakteri dengan aktivitas amilase tertinggi dikulturkan dalam media cair dengan dua sumber nitrogenyang berbeda, yaitu urea dan nitrat. PenentuanpH dan suhu optimum aktivitas enzim ini dilakukan pada rentang pH 4sampai 7 dan suhu 35sampai 65 ºC.Tiga isolat penghasil amilase diperoleh dalam penelitian ini. Isolat M1 yang memiliki aktivitas tertinggi dikarakterisasi berdasarkan uji katalasedan uji pewarnaan Gram. Hasilnya menunjukkan bahwa isolat M1 termasukgenus Proteus sp. Pada kondisi optimum (suhu 45oC dan pH 7), aktivitas amilase pada media nitrat adalah 0,791 U/mL, lebih kurang 18 kali lebih tinggi dibanding aktivitas pada media urea (0,041","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85572262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asosiasi Glomus sp. dan Gigaspora margarita pada bibit Aquilaria malaccensis","authors":"Endah Susilowati, Melya Riniarti, M. Rini","doi":"10.22302/iribb.jur.mp.v87i2.342","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v87i2.342","url":null,"abstract":"AbstractAgarwood (Aquilaria malaccensis) is one of the non-timber forest products that has high economic value. However, this plant grows very slowly.  Therefore, seed technology input is needed, such as addition of arbuscular mycorrhizal fungi (AMF). The objectives of this research were to study the effect of AMF inoculum i.e. Glomus sp., Gigaspora margarita and mixture of both species on agarwood growth and to determine the best AMF type for agarwood seedlings growth. The experimental research design used was a completely randomized design with three treatments, namely Glomus sp. (G), G. margarita (Gi) and a mixture of Glomus sp. and G. margarita (GGi) and without AMF inoculation (K) with each treatment repeated 8 times. The number of AMF spores used was ± 300 spores/seedling, and applied at the time of transplanting from germination tray to the polybag. Data were processed using analysis of variance and least significant difference test (LSD). The results showed there was AMF colonization in agarwood roots after 12 weeks of inoculation with the highest percentage at 20.50% on G. margarita treatment. Overall, AMF improved the growth of agarwood seedlings. The best seedlings growth was in the treatment of mixed AMF (GGi). Increased growth was found in plant height (14.68 cm), seedling diameter (2.16 mm), leaf area (119.30 cm2), root volume (1.15 mL), total dry weight (0.83 g), and shoot root ratio (4.99).[Keywords: agarwood, A. malaccensis,AMF, G. margarita,Glomussp.]AbstrakGaharu (Aquilariamalaccensis) adalah salah satu hasil hutan bukan kayu yang memiliki nilai ekonomis tinggi. Namun tanaman ini memiliki pertumbuhan yang lambat, sehingga diperlukan input teknologi pembibitan berupa penambahan fungi mikoriza arbuskular (FMA). Penelitian bertujuan untuk mengetahui pengaruh pemberian FMA tunggal Glomussp., Gigaspora margaritadan campuran keduanya (Glomussp. dan G. margarita)terhadap pertumbuhan gaharu serta menentukan isolatyang menghasilkan pertumbuhan bibit gaharu yang lebih baik.  Rancangan percobaan yang digunakan adalah rancangan acak lengkap untuk menguji tiga perlakuan yaitu Glomussp. (G), G. margarita (Gi) dan campuran Glomus sp. dan G. margarita(GGi) serta satu kontrol (K) dengan  masing – masing perlakuan diulang 8 kali. Inokulum FMA yang digunakan memiliki kepadatan ±300 spora/bibit dan diinokulasikan saat bibit dipindahkan dari persemaian ke polybag. Data diolah menggunakan analisis varians dan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa ditemukan adanya kolonisasi FMA dalam akar gaharu setelah 12 minggu inokulasi dengan persentase kolonisasi tertinggi 20,50% oleh G. margarita.  Secara keseluruhan, pemberian FMA mampu meningkatkan pertumbuhan bibit gaharu. Pertumbuhan terbaik terjadi pada perlakuan FMA campuran (GGi).  Peningkatan pertumbuhan secara nyata dapat dilihat pada tinggi tanaman (14,68 cm), diameter bibit (2,16 mm), luas daun (119,30 cm2), volume akar (1,15 mL), bobot kering total (0,83 g) dan nisbah ta","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88357219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peningkatan hasil dan penekanan kejadian penyakit pada jagung manis (Zea mays var. Bonanza) dengan pemanfaatan biostimulan berbahan kitosan","authors":"Sri Wahyuni, Ciptadi Achmad Yusup, Deden Dewantara Eris, Soekarno Mismana Putra, Agustin Sri Mulyatni, S. Siswanto, P. Priyono","doi":"10.22302/iribb.jur.mp.v87i2.349","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v87i2.349","url":null,"abstract":"AbstractCorn, an important crop in Indonesia still has a low productivity, thus many efforts are required to fulfill its national demand. One of the solutions to improve corn yield is by applying biostimulant containing chitosan as an active ingredient. Chitosan has been proved to increase plant growth and resistance against diseases. The objective of this research was to study the effects of several chitosan formulas on the yield and diseases occurance in sweet corn (Zea mays var. Bonanza). The chitosan formulas tested were soluble liquid(SL), wettable powder (WP), nano chitosan (NN), and unformulated chitosan (CH). The experiment was arranged using a randomized block design with three replications. All chitosan formulas were applied by seeds soaking for 20 minutes, followed by foliar spraying on corn plants at three weeks after planting (WAP), with the concentration of 500 ppm (400 L/ha spray volume), every threeweeks until 9 WAP. Parameters observed were brix value, weight of corn cobs, weight of corn biomass, and plant diseases including downy leaves, leafblight and leaf rust. The results showed that NN formula increased the brix value up to 7%, the corn cob weight up to 49% and the biomass weight upto 34% compared to the control; whereas SL formula reduced the incidence of downy mildew by 53% at 3 WAP and leaf blight disease by 51% at 6 WAP. In addition, the incidence of corn leaf rust reduced 59-71% in corn plant subjected to all chitosan formulas. Based on the results, application of chitosan in NN formula was best in increasing yield, while in SL formula was best in reducing the incidence of important corn diseases.[Keywords: downy mildew, chitosan formula, seed treatment]AbstrakJagung sebagai salah satu komoditas pangan penting di Indonesia masih memiliki produktivitas yang rendah sehingga diperlukan usaha untuk memenuhi kebutuhan jagung nasional. Salah satu cara untuk meningkatkan hasil jagung adalah dengan aplikasi biostimulan yang mengandung bahan aktif kitosan. Kitosan telah terbukti mampu meningkatkan pertumbuhan dan daya tahantanaman terhadap penyakit. Penelitian ini bertujuan mempelajari pengaruh beberapa formula kitosan terhadap hasil dan kejadian penyakit pada tanaman jagung manis (Zea mays var. Bonanza). Formula kitosan yang diuji adalah cairan yang dapat larut (soluble liquid, SL), tepung yang dapat dibasahi (wettable powder, WP), nano kitosan (nano chitosan, NN), dan kitosan non formulasi (unformulated chitosan, CH). Percobaan dilakukan menggunakan rancangan acak kelompok (RAK) dengan tiga ulangan. Masingmasing formula kitosan tersebut diaplikasikan melalui perendaman benih selama 20 menit yangdiikuti dengan penyemprotan daun pada tanaman jagung berumur tiga minggu dengan konsentrasi 500 ppm (volume semprot 400 L/ha) yang dilakukan setiap tiga minggu sampai tanaman berumur sembilan minggu. Parameter yangdiamati adalah nilai brix, bobot tongkol jagung, bobot biomassa jagung, dan penekanan kejadian beberapa penyakit tanaman mel","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90844969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration of oil palm plantlets introduced by P5CS gene using Agrobacterium-mediated transformation","authors":"A. Budiani, Imam Bagus Nugroho, H. Minarsih, I. Riyadi","doi":"10.22302/iribb.jur.mp.v87i2.336","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v87i2.336","url":null,"abstract":"AbstrakCekaman kekeringan dapat mempengaruhi produktivitas tanaman perkebunan. Rekayasa genetika merupakan salah satu cara untuk meningkatkan produktivitas tanaman perkebunan penting seperti kelapa sawit. Tujuan dari penelitian ini adalah melakukan perekayasaan kelapa sawit melalui introduksi gen P5CS dengan transformasi berbasis Agrobacterium untuk meningkatkan ketahanan tanaman terhadap cekaman kekeringan. Pada penelitian ini perakitan kelapa sawit transgenik yang tahan terhadap cekaman kekeringan dilakukan melalui transformasi gen P5CS (Δ1-pyrroline-5-carboxylate synthetase) ke dalam kalus embriogenik (embryogenic calli – EC) menggunakan Agrobacterium. Plasmid pBI_P5CS yang membawa gen P5CS ditransfer dari Escherichia coli XL1 Blue ke Agrobacterium tumefaciens AGL1 melalui konjugasi. Selanjutnya klon Agrobacterium yang membawa plasmid pBI_P5CS digunakan untuk menginfeksi kalus embriogenik kelapa sawit dengan perlakuan 100 ppm asetosiringon. Kalus transforman diregenerasi pada media de Fossard (DF) yang ditambahkan 50 ppm kanamisin dan 250 ppm sefotaksim. Kalus transforman diseleksi melalui uji GUS dan metode PCR menggunakan primer NPTII dan P5CS1. Uji GUS dilakukan untuk menyeleksi kalus transforman yang ditunjukkan dengan reaksi positif pembentukan warna biru pada kalus yang berhasil ditransformasi dengan konstruk pBI_P5CS. Pengujian dengan menggunakan PCR memberikan hasil positif dengan adanya profil pita PCR pada visualisasi menggunakan pewarnaan SYBR Green, yang menunjukkan amplikon berukuran ~ 0,7 kb untuk gen NPTII dan ~ 0,4 kb untuk gen P5CS pada elektroforesis dengan gel agarosa. Hasil dari penelitian ini adalah diperolehnya kalus transforman terseleksi yang telah diregenerasi dan tumbuh menjadi planlet.[Kata kunci: cekaman kekeringan, Elaeis guineensis Jacq., rekayasa genetika, planlet]Abstract      Environmental abiotic stressors particularly drought has detrimental effects upon the productivity of estate crops. Increasing the crop tolerance towards drought stress through genetic engineering is one of the strategies employed to maintain steady productivity of valuable crop, i.e. oil palm. The aim of this study was to engineer oil palm with a better tolerance towards drought by introducing P5CS (Δ1-pyrroline-5-carboxylate synthetase) gene via Agrobacterium–mediated transformation into embryogenic calli (EC). The pBI_P5CS plasmid harboring P5CS gene was transferred from Escherichia coli XL1 Blue to Agrobacterium tumefaciens AGL1 by conjugation. The positive clone of transformed Agrobacterium was then used to infect oil palm EC by the addition of 100 ppm acetosyringone. The transformed ECs were regenerated in the de Fossard (DF) media supplemented by 50 ppm kanamycin and 250 ppm cefotaxime followed by GUS assay and PCR-based screening using NPTII and P5CS1 primers. The positive EC clones were confirmed by GUS assay, which produced blue coloration on positive transformed oil palm EC. A positive result of PCR screenings was depicted by PC","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87568449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pelarutan P dan K dari batuan leusit dan apatit menggunakan kombinasi senyawa humat-BPF-BPK","authors":"Mohammad Jimmy Kurnianta, T. Setiawati, J. Jayus","doi":"10.22302/iribb.jur.mp.v87i2.330","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v87i2.330","url":null,"abstract":"AbstractDue to the limited availability of potassium and phosphate in soil and the high price of their synthetic mineral fertilizers, the importance of minerals-solubilizing microorganismshas been increasingly significant aimed at sustainable agriculture achievement. Apatite and leucite are considerably abundant minerals; however, their solubility is low. This study was aimed to study the effects of the combination of phosphate-and potassium-solubilizing bacteria (PhSB and PSB) and humic acid of cassava as bioleaching agents in the solubilization process of potassium and phosphate from agromineral material. Some leucite agromineral materials were obtained from Situbondo and Pati, while apatite materials were obtained from Tuban and Ciamis, Indonesia. The minerals were treated with 2.10 x 107CFU/g PhSB and 1.61 x 107CFU/g PSB isolates, combined with 100 ppm organic C humic compounds from cassava as the media. The minerals solubility was monitored every two weeks for 12 weeks ofobservation, which includes the concentration of dissolved K and P, as well as the pH of the media. The presence of organic acids was observed to monitor the bacterial activity by using HPLC, while the physical changes of the rock surface due to bacterial dissolution were scanned by using scanning electron microscopy (SEM). The results showed that the highest dissolution of P was recorded at week 4 (344.23 ppm) released from Tuban apatite with the combination of PhSB and PSB under the humic compound.In contrast, the highest K dissolution was obtained at week 6 for Situbondo leucite (44.21 me/100 g) with a combination of humic cassava compound and PSB only. Statistical analyses showed a mark different on both minerals for dissolution of K and P. The SEM result indicates breakage of the rock surface after a three month observation period indicating that the dissolution minerals occurred. Selected organic acids such as citric, ferulic, coumaric, syringic, and malic acids were detected during the treatment.[Keywords: leucite, apatite, humic compounds, organic acid] Abstrak     Ketersediaan kalium (K) dan fosfat (P) dalam tanah terbatas,danharga pupuk sintetik relatif mahal, menjadikan mikroorganismepelarut mineral memegang peranan penting dalam menunjang pertanian berkelanjutan.Batuan mineral apatit dan leusitmerupakan sumber daya mineral dengan ketersediaan tinggi tetapi mempunyai kelarutan mineral rendah. Penelitian bertujuan untukmempelajari pengaruh kombinasi bakteri pelarut fosfat (BPF), bakteri pelarut kalium (BPK), dan humatsingkong sebagai agen bioleachingdalam proses pelarutan K dan P dari bahan agromineral. Bahan agromineral leusitdiperoleh dariKabupatenSitubondo dan Pati, sedangkan bahan apatit berasal dari Kabupaten Tuban dan Ciamis, Indonesia. Bahan mineral diperlakukan dengan 2,10 x107CFU/gBPF dan 1,61 x 107CFU/gBPK, dikombinasikan dengan 100 ppm C organik senyawa humatdari singkong sebagai media. Kelarutan mineral diamati setiap dua minggu sekali selama 12 minggu ","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76127771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aktivitas hidrolisat protein terhadap perkecambahan dan pertumbuhan awal kacang hijau (Vigna radiata)","authors":"Fauziatul Fitriyah, Irma Kresnawaty, D. Santoso","doi":"10.22302/iribb.jur.mp.v87i2.340","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v87i2.340","url":null,"abstract":"AbstractPlant bostimulant application have proven to improve field productivity to meet genetic potential. Protein hydrolisates has been utilized as plant biostimulant to increase crops productiviy and yield. Protein hydrolysate from organic waste or by product is highly potential for plant biostimulant, since it is efficiently turn the waste into high value product.. Trash fish meal (TI) and chicken feather meal (TB) have high protein content and are potential as plant biostimulant. Application of protein hydrolisates as biostimulant has been acclaimed to improve plant growth. The aim of this study wasto evaluate the activity of protein hydrolysates from TB and TI on germination and early growth of mung bean. Hydrolysis was conducted under high temperature and pressure in acid condition. Application of protein hydrolysates under various concentrations: 5, 10, and 20 ppm. The effect of protein hydrolysates were evaluated on seed germination and root and colleoptile fresh weight. Chemical analysis was performed to measure nitrogen content in the materials. The result showed that germination at 10 ppm after 7 hours incubation of protein hydrolysate from TI and TB. Under TI hydrolysate germination was increase by 191.7%, from 21.7% blank solution to 63.3% of treatment. While under protein hydrolysate from TB increased by 99.5%, from 21.7% of blank solution to 43.3%. Activity of TB hydrolysate toward root and colleoptile growth was higher than in TI hydrolysate. Root growth was higher than colleoptile under all treatments. Protein hydrolysates of TI and TB could improve germination and early growth of mung bean and is highly potential as plant biostimulant.[Keywords: plant biostimulant, protein hydrolysis, plant growth, Vigna radiata]AbstrakAplikasi biostimulan tanaman terbukti mampu meningkatkan produktivitas riil di lapang sehingga mendekati potensi genetik tanaman. Hidrolisat protein telah dimanfaatkan sebagai biostimulan tanaman untuk meningkatkan produktivitas dan hasil panen berbagai tanaman. Hidrolisat protein dari limbah atau produk samping sangat potensial dikembangkan sebagai biostimulan tanaman karena mampu secara efisien mengubah sampah menjadi produk berharga. Tepung ikan rucah (TI) dan tepung bulu ayam (TB) adalah produk samping usaha perikanan dan peternakan dengan kandungan protein tinggi yang sangat potensial dikembangkan menjadi biostimulan untuk meningkatkan pertumbuhan tanaman. Tujuan penelitian ini adalah untuk mengetahui aktivitas hidrolisat protein dari TI dan TB terhadap perkecambahan dan pertumbuhan awal kacang hijau. Hidrolisis dilakukan pada suhu dan tekanan tinggi dalam kondisi asam. Hidrolisat yang diperoleh selanjutnya diaplikasikan pada benih kacang hijau pada konsentrasi 5, 10, dan 20 ppm. Parameter yang diamati berupa persentase perkecambahan dan pertumbuhan akar dan koleoptil semai. Analisis kimia dilakukan untuk memberikan data pendukung berupa kadar nitrogen pada kedua bahan baku. Hasil penelitian menunjukkan terja","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86247430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pemurnian alfa-selulosa dari baglog bekas jamur tiram putih (Pleurotus ostreatus) menggunakan NaOH dan hidrolisis sulfat (Purification of alpha-cellulose from ex-baglog of white oyster mushroom (Pleurotus ostreatus) using NaOH and sulfate hydrolysis)","authors":"H. Hanif, Tri Panji, Firda Dimawarnita, I. Artika","doi":"10.22302/IRIBB.JUR.MP.V87I1.325","DOIUrl":"https://doi.org/10.22302/IRIBB.JUR.MP.V87I1.325","url":null,"abstract":"Oil Palm Empty Fruit Bunches (OPEFB) are the largest cellulose waste produced by oil palm plantations. Each processing of one ton of Fresh Fruit Bunch (FFB) will produce OPEFB as much as 22-23% or 220-230 kg. OPEFB waste has not been utilized optimally by most palm oil mills and communities. It was reported that OPEFB contains 32.57% cellulose, 27.70% hemicellulose, and 26.49% lignin. The purity of cellulose component in OPEFB can be increased by delignification and hydrolysis of OPEFB. This research aims to produce alpha-cellulose from OPEFB with the highest purity level. The stages of the process include biologically delignification using white oyster mushroom (Pleurotus ostreatus), chemical delignification using 17,5% NaOH solution, and combination of treatment using solution of 17,5% NaOH and hydrolysis using 10% H2SO4solution. The analysis was carried out using the Fourier Transform Infrared Spectroscopy (FTIR). The best result of alpha-cellulose purity was by the treatment of delignified NaOH baglog resulting in 97.43% alpha-cellulose content, 4.47% pentosan hemicellulose level, and undetectable lignin levels.The results of FT-IR spectrum from alpha-cellulose obtained from OPEFB has shown functional groups contained in alpha-cellulose polymers including O-H groups at wave number 3289 cm-1, C-H groups at wave number 2901 cm-1, and functional groups C-O at wave number 1372 cm-1.[Key words: hydrolysis, FT-IR, mushroom, bleaching, waste]  Abstrak Tandan kosong kelapa sawit (TKKS) adalah limbah terbesar yang dihasilkan oleh perkebunan kelapa sawit. Setiap pengolahan satu ton tandan buah segar (TBS) akan dihasilkan TKKS sebanyak 22-23% atau 220-230 kg. Limbah TKKS belum dimanfaatkan secara optimaloleh sebagian besar pabrik kelapa sawit (PKS) dan masyarakat. Dari hasil analisis kimiakomposisi selulosa, hemiselulosa, dan lignin pada TKKSsecara berturut-turut ialah ; 32,57%, 27,70%, 26,49%. Kandungan komponen selulosa dalam TKKSdapat ditingkatkan kemurniannya dengan cara delignifikasi dan hidrolisis. Penelitian ini bertujuan pemurnian alfa–selulosa dari TKKS dengan tingkat kemurnian tertinggi.Tahapan pembuatan alfa-selulosameliputi delignifikasi secara biologis menggunakan jamur tiramputih(Pleurotus ostreatus), delignifikasi secara kimia menggunakan larutan NaOH 17,5%, dan kombinasi perlakuan menggunakan NaOH 17,5% dan larutanH2SO410%. Analisis dilakukan menggunakanFourier Transform Infrared Spektroscopy (FT-IR). Hasil pemurnian alfa-selulosa terbaik ialah melalui sisa baglog terdelignifikasi NaOH menghasilkan kadar alfa-selulosa 97.43%, kadar pentosan hemiselulosa 4.47%, serta kadar lignin tidak terdeteksi. Hasil spektrumFT-IR darialfa–selulosayang diperoleh dari TKKStelahmenunjukkan gugus-gugus fungsi yangterdapat di dalam polimeralfa–selulosadiantaranyagugus O-H pada bilangan gelombang 3289 cm-1,  gugus C-H pada bilangangelombang2901cm-1, dan gugus fungsi C-O pada bilangan gelombang 1372 cm-1. [Kata kunci: hidrolisis, FT-IR, jamur, pemutihan, lim","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"128 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87974861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aktivitas enzim ligninolitik Pleurotus ostreatus pada media yang mengandung TKKS dan aplikasinya untuk dekolorisasi zat warna (Activity of ligninolytic enzyme of Pleurotus ostreatus on media containing OPEFB and their application for dyes decolorization)","authors":"Firda Dimawarnita, Tri Panji","doi":"10.22302/IRIBB.JUR.MP.V87I1.328","DOIUrl":"https://doi.org/10.22302/IRIBB.JUR.MP.V87I1.328","url":null,"abstract":"Ligninolytic enzymes are known as extracellular enzymes produced by the white rot fungi class of basidiomycetes. One of the most well-known fungi of the white rot fungus isPleurotus ostreatus. The aim of this study to calculate the activity of ligninolytic enzymes in the growth media of Pleurotus ostreatusand their application in decolorization of dye colour. The ligninolytic enzyme extract obtained was used to decolorize bluedyes (MethyleneBlue)and red dyes(Congo Red). The highest laccase enzyme activity was in the first month of 0.35 U/mL with E1 media composition; the highest manganese peroxidase (MnP) enzyme activity was in the fourth month at 31.818 U / mL with E4 media composition; and the highest lignin peroxidase (LiP) enzyme activity was in the fifth month at 0.269 U / mL with E1 media composition. The enzyme extract obtained was then applied to decolorize red and blue dyes. Decolorization of dyes was measured using spectrophotometry with a blue wavelength of 470 nm and red 685 nm. The highest reduction in decolorization of blue dye and red dye was 12 hours with concentration of enzyme addition of 0.5%. Based on these results, ligninolytic enzymes potentiallyto be developed as bioactive agents for detergents.[Keywords: decolorization, laccase, mangan peroxidase, lignin peroxidase, spectrofotometry] AbstrakEnzim ligninolitik dikenal sebagai enzim ekstraseluler yang dihasilkan oleh jamur pelapuk putih golongan basidiomycetes. Salah satu jamur dari golongan jamur pelapuk putih yang banyak dikenal adalah Pleurotus ostreatus. Penelitian ini bertujuan menghitung aktivitas enzim ligninolitik pada media pertumbuhan jamur tiram  (Pleurotus ostreatus) dan aplikasinya dalam dekolorisasi zat warna.  Ekstrak enzim ligninolitik yang didapatkan kemudian dimanfaatkan untuk dekolorisasi zat warna biru(Methylene Blue)dan merah (Congo Red). Aktivitas enzim lakase tertinggi ada pada bulan pertama sebesar 0,35 U/mL dengan komposisi media E1; aktivitas enzim mangan peroksidase (MnP) tertinggi ada pada bulan keempat sebesar 31,818 U/mL dengan komposisi media E4; dan aktivitas enzim lignin peroksidase (LiP) tertinggi ada pada bulan kelima sebesar 0,269 U/mL dengan komposisi media E1. Ekstrak enzim yang didapat kemudian diaplikasikan untuk dekolorisasi zat warna merah dan biru. Dekolorisasi zat warna diukur menggunakan spektrofotometri dengan panjang gelombang biru pada 470 nm dan merah pada 685 nm. Penurunan dekolorisasi zat warna birudan zat warna merahtertinggi selama 12jam dengan konsentraasi penambahan enzim sebesar 0,5%.Berdasarkan hasil tersebut, enzim ligninolitik sangat potensial untuk dikembangkan sebagai agen bioaktif untuk deterjen.[Kata kunci: dekolorisasi, lakase, mangan peroksidase, lignin peroksidase,  spektrofotometri]","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88237806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peningkatan hasil panen kedelai (Glycine max L.) varietas Wilis dengan aplikasi biostimulan tanaman (Yield improvement of soybean (Glycine max L.) var. Wilis by the application of organic plant biostimulant)","authors":"D. A. Sari, Irma Kresnawati, .. Priyono, A. Budiani, D. Santoso","doi":"10.22302/IRIBB.JUR.MP.V87I1.295","DOIUrl":"https://doi.org/10.22302/IRIBB.JUR.MP.V87I1.295","url":null,"abstract":"The Indonesian government program of achieving self-sufficiency of soybean by 2020 requires technological innovations for the farmers. The use of plant biostimulant is an innovative strategy and proven previouslyto increase the productivity of several otherfood crops. The aim of this study was to analyze the effect of PPBBI biostimulant on the growth, productivity and quality of a Wilis variety of soybean under greenhouse conditions. PPBBI biostimulant at10 ppm and 20 ppm, was applied using foliar spray method with 20 mL volume to each plant. The applications were carried out 1 time, 2 times and 3 times. Six biostimulant treatments showed significant positive effects on the vegetative growth rate, generative organ development, and yield. The biostimulant accelerated vegetative growth to enter the generative phase earlier than that of in the control plants. The period of generative organs maturity required for treated plants was 7-14 d shorter than that of in the control plants so that the harvest period was 21 d shorter. Weight per 100 seeds of the P2-3 (application 3 times at 20 ppm); P2-2 (application 2 times at 20 ppm); P2-1 (application 1 time at 20 ppm) and P1-3 (application 3 times at 10 ppm) treatments were 20.16 g; 17.65 g; 18.89 g and 16.89 g respectively with no significant difference, while the control plants was only 11.60 g. Based on the results of all parameters e.g. average number of seeds, average weight per seed, and potential for yield improvement, the treatment of P1-3 (application 3 times at 10 ppm) was the best treatment with potential yield increase by 59.06% and oil content by 11.37%.[Key words: generative, organic biostimulant, productivity, vegetative]  AbstrakProgram pemerintah Indonesia dalam pencapaian swasembada kedelai pada tahun 2020 membutuhkan dukungan inovasi teknologi yang aplikatif untuk para petani. Biostimulan tanaman merupakan salah satu teknologi yang strategis dan terbukti dapat meningkatkan produktivitas beberapa tanaman pangan. Tujuan penelitian ini adalah untuk melakukan analisis pengaruh aplikasi biostimulan PPBBI terhadap partum-buhan, produktivitas dan kualitas hasil panen kedelai varietas Wilis pada kondisi rumah kaca. Biostimulan PPBBI dengan variasi konsentrasi   10 ppm dan 20 ppm, diaplikasikan pada tanaman kedelai dengan metode penyemprotan lewat daundengan volume 20 mL per tanaman.Penyemprotan dilakukan sebanyak 1 kali; 2 kali dan 3 kali aplikasi. Enam perlakuan biostimulanyang diujikan menunjukkan pengaruh positif yang signifikan pada kecepatan pertumbuhan vegetatif, stimulasi perkembangan organ generatif dan peningkatan hasil panen. Biostimulan PPBBI mempercepat laju pertumbuhan vegetatif untuk memasuki fase generatif lebih awal dibandingkan tanaman kontrol. Masa perkembangan dan pemasakan organ generatif polong pada tanaman perlakuan menjadi lebih pendek 7-14 hari dibandingkan tanaman kontrol sehingga secara keseluruhan masa panen tanaman perlakuan lebih singkat 21 hari. Bobot per 100 biji t","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86853275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}