eNeuro最新文献_第6页

Decoding Visual Spatial Attention Control.

IF 2.7 3区医学

eNeuro Pub Date : 2025-03-03 Print Date: 2025-03-01 DOI: 10.1523/ENEURO.0512-24.2025

Sreenivasan Meyyappan, Abhijit Rajan, Qiang Yang, George R Mangun, Mingzhou Ding

{"title":"Decoding Visual Spatial Attention Control.","authors":"Sreenivasan Meyyappan, Abhijit Rajan, Qiang Yang, George R Mangun, Mingzhou Ding","doi":"10.1523/ENEURO.0512-24.2025","DOIUrl":"10.1523/ENEURO.0512-24.2025","url":null,"abstract":"In models of visual spatial attention control, it is commonly held that top-down control signals originate in the dorsal attention network, propagating to the visual cortex to modulate baseline neural activity and bias sensory processing. However, the precise distribution of these top-down influences across different levels of the visual hierarchy is debated. In addition, it is unclear whether these baseline neural activity changes translate into improved performance. We analyzed attention-related baseline activity during the anticipatory period of a voluntary spatial attention task, using two independent functional magnetic resonance imaging datasets and two analytic approaches. First, as in prior studies, univariate analysis showed that covert attention significantly enhanced baseline neural activity in higher-order visual areas contralateral to the attended visual hemifield, while effects in lower-order visual areas (e.g., V1) were weaker and more variable. Second, in contrast, multivariate pattern analysis (MVPA) revealed significant decoding of attention conditions across all visual cortical areas, with lower-order visual areas exhibiting higher decoding accuracies than higher-order areas. Third, decoding accuracy, rather than the magnitude of univariate activation, was a better predictor of a subject's stimulus discrimination performance. Finally, the MVPA results were replicated across two experimental conditions, where the direction of spatial attention was either externally instructed by a cue or based on the participants' free choice decision about where to attend. Together, these findings offer new insights into the extent of attentional biases in the visual hierarchy under top-down control and how these biases influence both sensory processing and behavioral performance.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Chronic Social Isolation Stress and Alcohol on the Reinforcing Properties of Ketamine in Male and Female Rats.

IF 2.7 3区医学

eNeuro Pub Date : 2025-03-03 Print Date: 2025-03-01 DOI: 10.1523/ENEURO.0452-24.2025

Sarah D Jennings, Devin P Hagarty, Jordan Logue, Michelle Crawford, Samantha K Saland, Mohamed Kabbaj

{"title":"Effects of Chronic Social Isolation Stress and Alcohol on the Reinforcing Properties of Ketamine in Male and Female Rats.","authors":"Sarah D Jennings, Devin P Hagarty, Jordan Logue, Michelle Crawford, Samantha K Saland, Mohamed Kabbaj","doi":"10.1523/ENEURO.0452-24.2025","DOIUrl":"10.1523/ENEURO.0452-24.2025","url":null,"abstract":"While ketamine, an NMDA receptor antagonist, is effective in treating major depression, studies have not addressed the safety of repeated ketamine infusions in depressed patients with comorbid alcohol use disorder (AUD). In this study, we aimed to determine whether a history of chronic social isolation and alcohol exposure alter the reinforcing properties of ketamine in male and female rats. Rats were pair-housed or socially isolated for 12 weeks and underwent intermittent access to 20% alcohol. Subsequently, rats underwent intravenous ketamine self-administration under a fixed ratio 1 schedule, followed by extinction training and one session of cue-induced reinstatement. Dendritic spine morphology was examined in the nucleus accumbens, an important area implicated in reward and motivation. Our results show that females self-administered more ketamine than males, a history of alcohol increased ketamine intake in females, and a history of isolation or alcohol independently increased ketamine intake in males. All experimental groups showed similar extinction patterns and reinstatement to ketamine cues. A pattern emerged similar to ketamine self-administration behaviors, where isolation increased the number of immature spines in males, a change that was attenuated in isolated alcohol drinkers, and a history of alcohol increased the number of immature spines in females. Our results suggest that a history of isolation and alcohol modulate the reinforcing properties of ketamine in a sex-dependent manner. This underscores the importance of considering sex differences and a history of alcohol use when employing ketamine to treat various psychopathologies, including major depression.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limiting Hearing Loss in Transgenic Mouse Models.

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-27 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0465-24.2025

Travis A Babola, Naomi Donovan, Sean S Darcy, Catalina D Spjut, Patrick O Kanold

{"title":"Limiting Hearing Loss in Transgenic Mouse Models.","authors":"Travis A Babola, Naomi Donovan, Sean S Darcy, Catalina D Spjut, Patrick O Kanold","doi":"10.1523/ENEURO.0465-24.2025","DOIUrl":"10.1523/ENEURO.0465-24.2025","url":null,"abstract":"Transgenic mice provide unprecedented access to manipulate and visualize neural circuits; however, those on a C57BL/6 background develop progressive hearing loss, significantly confounding systems-level and behavioral analysis. While outbreeding can limit hearing loss, it introduces strain variability and complicates the generation of complex genotypes. Here, we propose an approach to preserve hearing by crossing transgenic mice with congenic B6.CAST-Cdh23Ahl + mice, which maintain low-threshold hearing into adulthood. Widefield and two-photon imaging of the auditory cortex revealed that 2.5-month-old C57BL/6 mice exhibit elevated thresholds to high-frequency tones and widespread cortical reorganization, with most neurons responding best to lower frequencies. In contrast, Ahl+ C57BL/6 mice exhibited robust neural responses across tested frequencies and sound levels (4-64 kHz, 30-90 dB SPL) and retained low thresholds into adulthood. Our approach offers a cost-effective solution for generating complex genotypes and facilitates more interpretable systems neuroscience research by eliminating confounding effects from hearing loss.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal Clustering of Functional Ultrasound Signals at the Single-Voxel Level.

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-26 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0438-24.2025

Théo Lambert, Hamid Reza Niknejad, Dries Kil, Gabriel Montaldo, Bart Nuttin, Clément Brunner, Alan Urban

{"title":"Spatiotemporal Clustering of Functional Ultrasound Signals at the Single-Voxel Level.","authors":"Théo Lambert, Hamid Reza Niknejad, Dries Kil, Gabriel Montaldo, Bart Nuttin, Clément Brunner, Alan Urban","doi":"10.1523/ENEURO.0438-24.2025","DOIUrl":"10.1523/ENEURO.0438-24.2025","url":null,"abstract":"Functional ultrasound (fUS) imaging is a well-established neuroimaging technology that offers high spatiotemporal resolution and a large field of view. Typical strategies for analyzing fUS data comprise either region-based averaging, typically based on reference atlases, or correlation with experimental events. Nevertheless, these methodologies possess several inherent limitations, including a restricted utilization of the spatial dimension and a pronounced bias influenced by preconceived notions about the recorded activity. In this study, we put forth single-voxel clustering as a third method to address these issues. A comparison was conducted between the three strategies on a typical dataset comprising visually evoked activity in the superior colliculus in awake mice. The application of single-voxel clustering yielded the generation of detailed activity maps, which revealed a consistent layout of activity and a clear separation between hemodynamic responses. This method is best considered as a complement to region-based averaging and correlation. It has direct applicability to challenging contexts, such as paradigm-free analysis on behaving subjects and brain decoding.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse Adrenal Macrophages Are Associated with Pre- and Postsynaptic Neuronal Elements and Respond to Multiple Neuromodulators.

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-25 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0153-24.2025

Matthew D Whim

{"title":"Mouse Adrenal Macrophages Are Associated with Pre- and Postsynaptic Neuronal Elements and Respond to Multiple Neuromodulators.","authors":"Matthew D Whim","doi":"10.1523/ENEURO.0153-24.2025","DOIUrl":"10.1523/ENEURO.0153-24.2025","url":null,"abstract":"The adrenal medulla is packed with chromaffin cells, modified postganglionic sympathetic neurons that secrete the catecholamines, epinephrine and norepinephrine, during the fight-or-flight response. Sometimes overlooked is a population of immune cells that also resides within the gland but whose distribution and function are not clear. Here I examine the location of CD45+ hematopoietic cells in the mouse adrenal medulla and show the majority are F4/80+/Lyz2+ macrophages. These cells are present from early postnatal development and widely distributed. Anatomically they are associated with chromaffin cells, found aligned alongside synapsin-IR neuronal varicosities and juxtaposed to CD31-IR blood vessels. Using Lyz2cre-GCaMP6f mice to quantify calcium signaling in macrophages revealed these cells respond directly and indirectly to a wide variety of neuromodulators, including pre- and postganglionic transmitters and systemic hormones. Purinergic agonists, histamine, acetylcholine, and bradykinin rapidly and reversibly increased intracellular calcium. These results are consistent with a substantial resident population of innate immune cells in the adrenal medulla. Their close association with chromaffin cells and the preganglionic input suggests they may regulate sympatho-adrenal activity and thus the strength of the fight-or-flight response.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering Compromised Speech-in-Noise Intelligibility in Older Listeners: The Role of Cochlear Synaptopathy. 破译老年听众在噪音中受损的言语清晰度：耳蜗突触病的作用。

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-20 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0182-24.2024

Markus Garrett, Viacheslav Vasilkov, Manfred Mauermann, Pauline Devolder, John L Wilson, Leslie Gonzales, Kenneth S Henry, Sarah Verhulst

{"title":"Deciphering Compromised Speech-in-Noise Intelligibility in Older Listeners: The Role of Cochlear Synaptopathy.","authors":"Markus Garrett, Viacheslav Vasilkov, Manfred Mauermann, Pauline Devolder, John L Wilson, Leslie Gonzales, Kenneth S Henry, Sarah Verhulst","doi":"10.1523/ENEURO.0182-24.2024","DOIUrl":"10.1523/ENEURO.0182-24.2024","url":null,"abstract":"Speech intelligibility declines with age and sensorineural hearing damage (SNHL). However, it remains unclear whether cochlear synaptopathy (CS), a recently discovered form of SNHL, significantly contributes to this issue. CS refers to damaged auditory-nerve synapses that innervate the inner hair cells and there is currently no go-to diagnostic test available. Furthermore, age-related hearing damage can comprise various aspects (e.g., hair cell damage, CS) that each can play a role in impaired sound perception. To explore the link between cochlear damage and speech intelligibility deficits, this study examines the role of CS for word recognition among older listeners. We first validated an envelope-following response (EFR) marker for CS using a Budgerigar model. We then applied this marker in human experiments, while restricting the speech material's frequency content to ensure that both the EFR and the behavioral tasks engaged similar cochlear frequency regions. Following this approach, we identified the relative contribution of hearing sensitivity and CS to speech intelligibility in two age-matched (65-year-old) groups with clinically normal (n = 15, 8 females) or impaired audiograms (n = 13, 8 females). Compared to a young normal-hearing control group (n = 13, 7 females), the older groups demonstrated lower EFR responses and impaired speech reception thresholds. We conclude that age-related CS reduces supra-threshold temporal envelope coding with subsequent speech coding deficits in noise that cannot be explained based on hearing sensitivity alone.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-155 Inhibition Activates Wnt/β-Catenin Signaling to Restore Th17/Treg Cell Balance and Protect against Acute Ischemic Stroke. 抑制MicroRNA-155激活Wnt/β-catenin信号，恢复Th17/Treg平衡，预防急性缺血性卒中

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-20 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0347-24.2024

Wenli Huang, Quanlong Hong, Huimin Wang, Zhihua Zhu, Shujie Gong

{"title":"MicroRNA-155 Inhibition Activates Wnt/β-Catenin Signaling to Restore Th17/Treg Cell Balance and Protect against Acute Ischemic Stroke.","authors":"Wenli Huang, Quanlong Hong, Huimin Wang, Zhihua Zhu, Shujie Gong","doi":"10.1523/ENEURO.0347-24.2024","DOIUrl":"10.1523/ENEURO.0347-24.2024","url":null,"abstract":"Acute ischemic stroke (AIS) is a severe neurological disease associated with Th17/Treg cell imbalance and dysregulation of the Wnt/β-catenin signaling pathway. This study investigates whether miR-155 inhibition can activate Wnt/β-catenin signaling, improve Th17/Treg balance, and provide neuroprotection against stroke. We conducted a multilevel experimental design, including high-throughput sequencing, bioinformatics analysis, in vivo mouse models, and in vitro cell experiments. High-throughput sequencing revealed significant differential gene expression between the miR-155 antagomir-treated and control groups (BioProject: PRJNA1152758). Bioinformatics analysis identified key genes linked to Wnt/β-catenin signaling and Th17/Treg imbalance. In vitro experiments confirmed that miR-155 inhibition activated Wnt/β-catenin signaling and improved Th17/Treg ratios. In vivo studies demonstrated that miR-155 antagomir treatment provided significant neuroprotection against AIS. These findings suggest that targeting miR-155 could be a promising therapeutic strategy for stroke by modulating immune balance and key signaling pathways.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving Tracking of Selective Attention in Hearing Aid Users: The Role of Noise Reduction and Nonlinearity Compensation.

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-19 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0275-24.2025

Johanna Wilroth, Emina Alickovic, Martin A Skoglund, Carine Signoret, Jerker Rönnberg, Martin Enqvist

{"title":"Improving Tracking of Selective Attention in Hearing Aid Users: The Role of Noise Reduction and Nonlinearity Compensation.","authors":"Johanna Wilroth, Emina Alickovic, Martin A Skoglund, Carine Signoret, Jerker Rönnberg, Martin Enqvist","doi":"10.1523/ENEURO.0275-24.2025","DOIUrl":"10.1523/ENEURO.0275-24.2025","url":null,"abstract":"Hearing impairment (HI) disrupts social interaction by hindering the ability to follow conversations in noisy environments. While hearing aids (HAs) with noise reduction (NR) partially address this, the \"cocktail-party problem\" persists, where individuals struggle to attend to specific voices amidst background noise. This study investigated how NR and an advanced signal processing method for compensating for nonlinearities in Electroencephalography (EEG) signals can improve neural speech processing in HI listeners. Participants wore HAs with NR, either activated or deactivated, while focusing on target speech amidst competing masker speech and background noise. Analysis focused on temporal response functions to assess neural tracking of relevant target and masker speech. Results revealed enhanced neural responses (N1 and P2) to target speech, particularly in frontal and central scalp regions, when NR was activated. Additionally, a novel method compensated for nonlinearities in EEG data, leading to improved signal-to-noise ratio (SNR) and potentially revealing more precise neural tracking of relevant speech. This effect was most prominent in the left-frontal scalp region. Importantly, NR activation significantly improved the effectiveness of this method, leading to stronger responses and reduced variance in EEG data and potentially revealing more precise neural tracking of relevant speech. This study provides valuable insights into the neural mechanisms underlying NR benefits and introduces a promising EEG analysis approach sensitive to NR effects, paving the way for potential improvements in HAs.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are You Safe or Should I Go? How Perceived Trustworthiness and Probability of a Sexual Transmittable Infection Impact Activation of the Salience Network.

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-19 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0258-24.2024

Alexander Wolber, Stephanie N L Schmidt, Brigitte Rockstroh, Daniela Mier

{"title":"Are You Safe or Should I Go? How Perceived Trustworthiness and Probability of a Sexual Transmittable Infection Impact Activation of the Salience Network.","authors":"Alexander Wolber, Stephanie N L Schmidt, Brigitte Rockstroh, Daniela Mier","doi":"10.1523/ENEURO.0258-24.2024","DOIUrl":"10.1523/ENEURO.0258-24.2024","url":null,"abstract":"Functional imaging studies indicate that both the assessment of a person as untrustworthy and the assumption that a person has a sexually transmitted infection are associated with activation in regions of the salience network. However, studies are missing that combine these aspects and investigate the perceived trustworthiness of individuals previously assessed with high or low probability of a sexually transmitted infection. During fMRI measurements, 25 participants viewed photographs of people preclassified as having high or low HIV probability and judged their trustworthiness. In a postrating, stimuli were rated for trustworthiness, attractiveness, and HIV probability. Persons preclassified as HIV- in contrast to those preclassified as HIV+ were rated more trustworthy and with lower HIV probability. Activation in medial orbitofrontal cortex was higher for those rated and preclassified as HIV- than HIV+. Based on the individual ratings, but not the preclassification, there was significantly higher activation in the insula, amygdala, anterior cingulate cortex, and nucleus accumbens in response to untrustworthy than to trustworthy faces. Activation of the salience network occurred when a person was judged as untrustworthy, but not according to a preclassification. Activation in the medial orbitofrontal cortex, a structure associated with reward, was enhanced when a person was perceived as trustworthy and also when a person was preclassified with low HIV probability. Our findings suggest that trustworthiness and HIV- perception have consistency across samples, while the perception of risk and associated activation of the salience network has restricted cross-sample consistency.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple, Low-Cost Implant for Reliable Diaphragm EMG Recordings in Awake, Behaving Rats.

IF 2.7 3区医学

eNeuro Pub Date : 2025-02-19 Print Date: 2025-02-01 DOI: 10.1523/ENEURO.0444-24.2025

Taylor C Holmes, Jesus D Penaloza-Aponte, Alyssa R Mickle, Rachel L Nosacka, Erica A Dale, Kristi A Streeter

{"title":"A Simple, Low-Cost Implant for Reliable Diaphragm EMG Recordings in Awake, Behaving Rats.","authors":"Taylor C Holmes, Jesus D Penaloza-Aponte, Alyssa R Mickle, Rachel L Nosacka, Erica A Dale, Kristi A Streeter","doi":"10.1523/ENEURO.0444-24.2025","DOIUrl":"10.1523/ENEURO.0444-24.2025","url":null,"abstract":"Breathing is a complex neuromuscular process vital to sustain life. In preclinical animal models, the study of respiratory motor control is primarily accomplished through neurophysiologic recordings and functional measurements of respiratory output. Neurophysiologic recordings that target neural or muscular output via direct nerve recordings or respiratory muscle electromyography (EMG) are commonly collected during anesthetized conditions. While offering tight control of experimental preparations, the use of anesthesia results in respiratory depression, may impact cardiovascular control, eliminates the potential to record volitional nonventilatory behaviors, and can limit translation. Since the diaphragm is a unique muscle which is rhythmically active and difficult to access, placing diaphragm EMGs to collect chronic recordings in awake animals is technically challenging. Here, we describe methods for fabricating and implanting indwelling diaphragm EMG electrodes to enable recordings from awake rodents for longitudinal studies. These electrodes are relatively easy and quick to produce (∼1 h), are affordable, and provide high-quality and reproducible diaphragm signals using a tethered system that allows animals to ad libitum behave. This system is also designed to work in conjunction with whole-body plethysmography to facilitate simultaneous recordings of diaphragm EMG and ventilation. We include detailed instructions and considerations for electrode fabrication and surgical implantation. We also provide a brief discussion on data acquisition, material considerations for implant fabrication, and the physiological implications of the diaphragm EMG signal.","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0