eNeuroPub Date : 2025-08-28Print Date: 2025-08-01DOI: 10.1523/ENEURO.0566-24.2025
Aishwarya Segu, Shrutika Sansaria, Nisha N Kannan
{"title":"<i>Tequila</i>, the Serine Protease, Is Involved in Sleep-Dependent Memory Consolidation in <i>Drosophila</i>.","authors":"Aishwarya Segu, Shrutika Sansaria, Nisha N Kannan","doi":"10.1523/ENEURO.0566-24.2025","DOIUrl":"10.1523/ENEURO.0566-24.2025","url":null,"abstract":"<p><p>Sleep is a vital physiological phenomenon observed among almost all organisms. Although its exact purpose remains elusive, sleep has been linked to memory consolidation. In our present study, we investigated the role of sleep quality on sleep-dependent memory consolidation. Previous studies have shown that <i>tequila</i>, a serine protease, affects long-term memory (LTM) consolidation in flies. In the present study, we identified that the hypomorphic mutation in the <i>tequila</i> gene (<i>tequila<sup>f01792</sup></i> ) leads to increased daytime sleep fragmentation at a very early age in male flies. Intrigued by this observation, we delved into further understanding the role of <i>tequila</i> in sleep-dependent memory consolidation by manipulating sleep duration using pharmacological methods such as GABA-A agonist. Inducing sleep using GABA-A agonist resulted in improved sleep during the day. This further led to a significant improvement in the LTM of these flies when compared with the vehicle-treated flies. In conclusion, daytime-dependent sleep fragmentation is possibly one of the reasons behind the LTM deficit observed in <i>tequila<sup>f01792</sup></i> flies. Furthermore, we observed that these flies had disturbed sleep only during the daytime, whereas in the night the flies had increased sleep duration. The increased sleep fragmentation during the daytime possibly leads to memory impairment and rescuing sleep fragmentation partially rescues memory consolidation. These outcomes suggest that the <i>tequila</i> gene is involved in sleep-dependent memory consolidation.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-28Print Date: 2025-08-01DOI: 10.1523/ENEURO.0068-25.2025
Heather R Dial, G Nike Gnanateja
{"title":"Test-Retest Reliability of TRF-Derived Measures of Cortical Tracking of the Speech Envelope.","authors":"Heather R Dial, G Nike Gnanateja","doi":"10.1523/ENEURO.0068-25.2025","DOIUrl":"10.1523/ENEURO.0068-25.2025","url":null,"abstract":"<p><p>Cortical tracking of the speech envelope is an emerging, noninvasive measure of neurophysiological processing of speech that is being widely adopted. It demonstrates good ecological validity, as it allows researchers to study human processing of continuous, naturalistic speech containing dynamic spectrotemporal variations and rich linguistic content. While measures of cortical tracking have strong clinical and research applications, there is a lack of research documenting the reliability of these measures, including how they are affected by the stimulus and how the stimulus is represented, as well as electroencephalography (EEG) acquisition and analysis parameters. In this study, we measured the test-retest reliability of cortical tracking of the speech envelope across different stimuli (an audiobook vs a podcast), stimulus features (broadband envelope and its derivative, multiband envelope and its derivative), reference electrodes (average mastoid vs common average reference), and EEG frequency bands (delta, theta, full) in 26 neurotypical adults (20 female) to assess the potential for cortical tracking to serve as a reliable measure of speech processing. We also examined the amount of data required to achieve stable reliability estimates. We observed moderate to good test-retest reliability for most parameters with as little as 390 s of data, supporting the utility of cortical tracking of the speech envelope as a reliable tool for assessing speech processing. The findings of this study will allow researchers to more effectively design and implement studies on cortical tracking in neurotypical adults and adults with language disorders.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":"12 8","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-27Print Date: 2025-08-01DOI: 10.1523/ENEURO.0224-25.2025
Kiranmayi Vedantham, Longgang Niu, Ryan Ma, Liam Connelly, Anusha Nagella, Sijie Jason Wang, Zhao-Wen Wang
{"title":"<i>Track-A-Worm 2.0</i>: A Software Suite for Quantifying Properties of <i>C. elegans</i> Locomotion, Bending, Sleep, and Action Potentials.","authors":"Kiranmayi Vedantham, Longgang Niu, Ryan Ma, Liam Connelly, Anusha Nagella, Sijie Jason Wang, Zhao-Wen Wang","doi":"10.1523/ENEURO.0224-25.2025","DOIUrl":"10.1523/ENEURO.0224-25.2025","url":null,"abstract":"<p><p>Comparative analyses of locomotor behavior and cellular electrical properties between wild-type and mutant <i>Caenorhabditis elegans</i> are crucial for exploring the gene basis of behaviors and the underlying cellular mechanisms. Although many tools have been developed by research labs and companies, their application is often hindered by implementation difficulties or lack of features specifically suited for <i>C. elegans</i> Our system addresses these challenges with three key components: <i>WormTracker</i>, <i>SleepTracker</i>, and <i>Action Potential (AP) Analyzer</i> <i>WormTracker</i> accurately quantifies a comprehensive set of locomotor and body bending metrics, incorporates user-identified dorsal and ventral orientation based on microscopic observation, continuously tracks the animal using a motorized stage, and seamlessly integrates external devices, such as a light source for optogenetic stimulation. <i>SleepTracker</i> detects and quantifies sleep-like behavior in freely moving animals. <i>AP Analyzer</i> assesses the resting membrane potential, afterhyperpolarization level, and various AP properties, including threshold, amplitude, mid-peak width, rise and decay times, and maximum and minimum slopes. Importantly, it addresses the challenge of AP threshold quantification posed by the absence of a preupstroke inflection point. This system is potentially a valuable tool for many <i>C. elegans</i> research labs due to its powerful functionality and ease of implementation.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":"12 8","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-22Print Date: 2025-08-01DOI: 10.1523/ENEURO.0204-25.2025
Xin Wang, Caitlin Ribeiro, Anna Nilsson, Joan B Escobar, Bridget R Alber, John R Bethea, Vsevolod Y Polotsky, Matthew W Kay, Kathryn Schunke, David Mendelowitz
{"title":"Oxytocin Receptor Expression and Activation in Parasympathetic Brainstem Cardiac Vagal Neurons.","authors":"Xin Wang, Caitlin Ribeiro, Anna Nilsson, Joan B Escobar, Bridget R Alber, John R Bethea, Vsevolod Y Polotsky, Matthew W Kay, Kathryn Schunke, David Mendelowitz","doi":"10.1523/ENEURO.0204-25.2025","DOIUrl":"10.1523/ENEURO.0204-25.2025","url":null,"abstract":"<p><p>Autonomic imbalance-particularly reduced activity from brainstem parasympathetic cardiac vagal neurons (CVNs)-is a major characteristic of many cardiorespiratory diseases. Therapeutic approaches to selectively enhance CVN activity have been limited by the lack of defined, translationally relevant targets. Previous studies have identified an important excitatory synaptic pathway from oxytocin (OXT) neurons in the paraventricular nucleus of the hypothalamus to brainstem CVNs, suggesting that OXT could provide a key selective excitation of CVNs. In clinical studies, intranasal OXT has been shown to increase parasympathetic cardiac activity, improve autonomic balance, and reduce obstructive event durations and oxygen desaturations in obstructive sleep apnea patients. However, the mechanisms by which activation of hypothalamic OXT neurons, or intranasal OXT, enhance brainstem parasympathetic cardiac activity remain unclear. CVNs are located in two cholinergic brainstem nuclei: nucleus ambiguus (NA) and dorsal motor nucleus of the vagus (DMNX). In this study, we characterize the colocalization of OXT receptors (OXTRs) in both CVNs and non-CVN cholinergic neurons in the male and female mouse NA and DMNX nuclei. We found that OXT receptors are highly expressed in CVNs in the DMNX, but not in the NA. OXT increases the firing of DMNX CVN, with no effect on NA CVNs. Selective chemogenetic excitation of OXTR+ CVNs in the DMNX-achieved by a combination of Cre- and flp-dependent DREADD expression-evoked a rapid and sustained bradycardia. These findings suggest that activation of DMNX CVNs expressing OXTR with oxytocin may represent a novel translational therapeutic target for restoring autonomic balance in cardiorespiratory disorders.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-22Print Date: 2025-08-01DOI: 10.1523/ENEURO.0046-25.2025
Sarah N Ebert, Christine Eisner, Konstantina Karamboulas, Louis-Philippe Bernier, David R Kaplan, Brian A MacVicar, Freda D Miller
{"title":"Single-Cell Approaches Define the Murine Leptomeninges: Cortical Brain Interface as a Distinct Cellular Neighborhood Composed of Neural and Non-neural Cell Types.","authors":"Sarah N Ebert, Christine Eisner, Konstantina Karamboulas, Louis-Philippe Bernier, David R Kaplan, Brian A MacVicar, Freda D Miller","doi":"10.1523/ENEURO.0046-25.2025","DOIUrl":"10.1523/ENEURO.0046-25.2025","url":null,"abstract":"<p><p>The interface barrier between the brain surface and the adjacent meninges is important for regulating exchanges of fluid, protein, and immune cells between the CNS and periphery. However, the cell types that form this important interface are not yet fully defined. To address this limitation, we used single-cell RNA sequencing (scRNA-seq) and single-cell spatial transcriptomics together with morphological lineage tracing and immunostaining to describe the cell types forming the interface barrier of the adult murine cortex. We show that the cortical interface is composed of three major cell types, leptomeningeal cells, border astrocytes, and tissue-resident macrophages. On the nonparenchymal side, the interface is composed of transcriptionally distinct PDGFRα-positive leptomeningeal cells that are intermingled with macrophages. This leptomeningeal layer is lined by a population of transcriptionally distinct border astrocytes. The interface neighborhood is rich in growth factor mRNAs, including many leptomeningeal ligands predicted to act on both the border astrocytes and macrophages. On the CNS side of the interface is the relatively cell-sparse cortical layer 1 containing interneurons, microglia, parenchymal astrocytes, oligodendrocyte precursor cells, and oligodendrocytes. Except for the border astrocytes, layer 1 cells are not closely associated with the interface, suggesting that secreted ligands may be the major way the brain interface communicates with the underlying cortical parenchyma. Thus, our data provide a molecular/cellular resource describing the brain interface cell types and their interactions, thereby enabling future studies investigating how this distinct cellular compartment regulates CNS:periphery interactions.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-21Print Date: 2025-08-01DOI: 10.1523/ENEURO.0582-24.2025
Touhid Feghhi, Roberto X Hernandez, Olena Mahneva, Carlos D Oliva, Gregory T Macleod
{"title":"Bichromatic Exon-Reporters Reveal Voltage-Gated Ca<sup>2+</sup>-Channel Splice-Isoform Diversity across <i>Drosophila</i> Neurons In Vivo.","authors":"Touhid Feghhi, Roberto X Hernandez, Olena Mahneva, Carlos D Oliva, Gregory T Macleod","doi":"10.1523/ENEURO.0582-24.2025","DOIUrl":"10.1523/ENEURO.0582-24.2025","url":null,"abstract":"<p><p>Every neuron contains the same genomic information, but its complement of proteins is the product of countless neuron-specific steps including pre-mRNA splicing. Despite advances in RNA sequencing techniques, pre-mRNA splicing biases that favor one isoform over another are largely inscrutable in live neurons in situ. Here, in <i>Drosophila</i>, we developed bichromatic fluorescent reporters to investigate alternative splicing of <i>cacophony</i> (<i>cac</i>)-a gene that codes the pore-forming α<sub>1</sub> subunit of the primary neuronal voltage-gated Ca<sup>2+</sup> channel (VGCC). These reporters revealed a neuron-specific pattern of exon biases, highly consistent from one animal to the next, suggesting that each neuron splices a unique and consistent portfolio of VGCC isoforms. Stereotypical patterns were observed within motor neurons and multidendritic sensory neurons of female larvae and also within mushroom body Kenyon cells of female adults. In a validation step, we demonstrated that exon splice bias reporting was not dependent on the choice of fluorophores. Additionally, functional properties of the female larval motor neuron terminals could be generally reconciled with the functional properties predicted for the reported exon bias. The application of this technology to a large gene such as <i>cac</i> provides a precedence for effective exon-reporter design for other <i>Drosophila</i> genes.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-21Print Date: 2025-08-01DOI: 10.1523/ENEURO.0140-25.2025
Murtaza Haidary, Elham Akbari, Mohammad Edris Amiri, Khan Baba Ghazanfar, Mohammad Tariq Anwary, Mohammad Jalal Nazari, Mohammad Hussain Khadimi
{"title":"Thiamine Mitigates Nicotine Withdrawal Effects in Adolescent Male Rats: Modulation of Serotonin Metabolism, BDNF, Oxidative Stress, and Neuroinflammation.","authors":"Murtaza Haidary, Elham Akbari, Mohammad Edris Amiri, Khan Baba Ghazanfar, Mohammad Tariq Anwary, Mohammad Jalal Nazari, Mohammad Hussain Khadimi","doi":"10.1523/ENEURO.0140-25.2025","DOIUrl":"10.1523/ENEURO.0140-25.2025","url":null,"abstract":"<p><p>Adolescent nicotine use is particularly concerning due to increased susceptibility to long-term effects and dependence during this critical developmental period. This study investigates the therapeutic effects of thiamine on nicotine withdrawal-induced anxiety, anhedonia, and depression in rats. Adolescent rats received nicotine (2 mg/kg, s.c.) for 21 d, followed by 21 d of withdrawal. Thiamine (25 or 50 mg/kg, i.p.) was administered during exposure and withdrawal. Behavioral assessments were used to evaluate anxiety- and depressive-like symptoms, and biochemical analyses measured oxidative stress markers, serotonin levels, MAO activity, BDNF, and GFAP as indicators of neuroinflammation in the prefrontal cortex. Nicotine withdrawal significantly elevated anxiety-, depression-, and anhedonia-like behaviors, increased oxidative stress, and upregulated MAO-A activity and GFAP expression, indicating neuroinflammatory effects. Notably, thiamine administration during both nicotine exposure and withdrawal effectively alleviated these behavioral impairments, restored serotonin levels, reduced oxidative stress markers, and mitigated the increase in GFAP expression. Additionally, thiamine alone has been shown to alleviate anxiety- and depressive-like behaviors. This study highlights thiamine's potential as a promising intervention for managing psychological distress associated with nicotine withdrawal. Given the high prevalence of adolescent nicotine use and its associated mental health challenges, further research on thiamine's mechanisms and therapeutic potential is warranted to improve treatment strategies during this critical developmental stage.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-19Print Date: 2025-08-01DOI: 10.1523/ENEURO.0252-25.2025
Molly C Shallow, Lucy Tian, Bryan T Higashikubo, Hudson Lin, Katheryn B Lefton, Siyu Chen, Joseph D Dougherty, Joe P Culver, Mary E Lambo, Keith B Hengen
{"title":"Experience-Dependent Intrinsic Plasticity in Layer IV of Barrel Cortex at Whisking Onset.","authors":"Molly C Shallow, Lucy Tian, Bryan T Higashikubo, Hudson Lin, Katheryn B Lefton, Siyu Chen, Joseph D Dougherty, Joe P Culver, Mary E Lambo, Keith B Hengen","doi":"10.1523/ENEURO.0252-25.2025","DOIUrl":"10.1523/ENEURO.0252-25.2025","url":null,"abstract":"<p><p>The development of motor control over sensory organs is a critical milestone, enabling active exploration and shaping of the sensory environment. Whether the onset of sensory organ motor control directly influences the development of corresponding sensory cortices remains unknown. Here, we confirm and exploit the late onset of whisking behavior in mice to address this question in the somatosensory system. Using ex vivo electrophysiology, we describe a transient increase in the intrinsic excitability of excitatory neurons in layer IV of the barrel cortex, which processes whisker input, immediately following the onset of active whisking on postnatal days 13 and 14. This increase in neuronal gain is specific to layer IV, independent of changes in synaptic strength, and requires prior sensory experience. Further, these effects are not expressed in inhibitory interneurons in barrel cortex. The transient increase in excitability is not evident in layer II/III of barrel cortex or in the visual cortex upon eye opening, suggesting a unique interaction between the development of active sensing and the thalamocortical input layer in the somatosensory isocortex. Predictive modeling indicates that, immediately following the onset of active whisking, changes in active membrane conductances alone can reliably distinguish neurons in control but not whisker-deprived hemispheres. Our findings demonstrate an experience-dependent, lamina-specific refinement of neuronal excitability tightly linked to the emergence of active whisking. This transient increase in the gain of the thalamic input layer coincides with a critical period for synaptic plasticity in downstream layers, suggesting a role in cortical maturation and sensory processing.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
eNeuroPub Date : 2025-08-18Print Date: 2025-08-01DOI: 10.1523/ENEURO.0219-25.2025
Jacob M Reeves, Erwin Arias-Hervert, Gracianne E Kmiec, William T Birdsong
{"title":"Excitatory Synaptic Transmission Is Differentially Modulated by Opioid Receptors along the Claustrocingulate Pathway.","authors":"Jacob M Reeves, Erwin Arias-Hervert, Gracianne E Kmiec, William T Birdsong","doi":"10.1523/ENEURO.0219-25.2025","DOIUrl":"10.1523/ENEURO.0219-25.2025","url":null,"abstract":"<p><p>The anterior cingulate cortex (ACC) plays a pivotal role in processing pain and emotion, communicating with both cortical and subcortical regions involved in these functions. The claustrum (CLA), a subcortical region with extensive connectivity to the ACC, also plays a critical role in pain perception and consciousness. Both ACC and CLA express Kappa (KOR), Mu (MOR), and Delta (DOR) opioid receptors, yet whether and how opioid receptors modulate this circuit are poorly understood. This study investigates the effects of opioid receptor activation on glutamatergic signaling in CLA→ACC circuitry using spatial transcriptomics, brain slice electrophysiology, optogenetics, and pharmacological approaches in mice of both sexes. Our results demonstrated that excitatory synaptic transmission generated by the CLA onto Layer 5 pyramidal (L5 PYR) cells in the ACC are reduced by KOR, MOR, and DOR agonists. However, only KOR agonists reduce monosynaptic transmission from the CLA onto L5 ACC PYR cells, highlighting the unique role of KOR in modulating the CLA→ACC pathway. MOR and DOR agonists only reduced slower, longer-latency recurrent excitatory responses. These findings provide new insights into how opioid receptors regulate the claustrocingulate circuit and demonstrate the distinct, receptor-specific modulation of synaptic transmission within this network.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12360626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}