eNeuro最新文献

{"title":"High-Order Information Analysis of Epileptogenesis in the Pilocarpine Rat Model of Temporal Lobe Epilepsy.","authors":"Morteza Mirjebreili, Josu Martinez de Aguirre Ibarreta, Daniele Marinazzo, Laetitia Chauvière","doi":"10.1523/ENEURO.0403-24.2025","DOIUrl":"10.1523/ENEURO.0403-24.2025","url":null,"abstract":"<p><p>Temporal lobe epilepsy (TLE) is a devastating disease, often pharmacoresistant and with a high prevalence of 1% worldwide. There are a few disease-modifying therapies; thus, prevention has become a health priority. The overarching goal of this research project is to highlight the system's dynamics at different stages before TLE onset to identify an early shift in network dynamics trajectory toward disease onset. Researchers often investigate collective brain activity by tracking dynamical interactions of the signal recorded at multiple sites. However, these interactions are usually only computed between pairs of brain regions, at the risk of missing simultaneous interactions of three or more areas, an aspect that is crucial in a networked disease such as TLE. We thus propose to track, on a rich dataset of electrophysiological brain signals recorded within the temporal lobe (TL) of adult male Wistar Han rats, the formation and dissolution of high-order informational multiplets in time during distinct natural behaviors in an animal model of TLE. We identified the informational content of the multiplets as synergistic or redundant. Results identified an early transition of synergistic and redundant multiplets ahead of TLE onset with the predominant involvement of four TL brain regions in generating theta (4-12 Hz) activity. This shift has been shown predominantly during exploration, a theta-dependent behavior, less during rest and sleep. This specific change suggests a shift in communication from an integrated to a segregated network toward TLE onset.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parallel Gene Expression Changes in Ventral Midbrain Dopamine and GABA Neurons during Normal Aging.","authors":"Ana Luiza Drumond-Bock, Harris E Blankenship, Kevin D Pham, Kelsey A Carter, Willard M Freeman, Michael J Beckstead","doi":"10.1523/ENEURO.0107-25.2025","DOIUrl":"10.1523/ENEURO.0107-25.2025","url":null,"abstract":"<p><p>The consequences of aging can vary dramatically between different brain regions and cell types. In the ventral midbrain, dopaminergic neurons develop physiological deficits with normal aging that likely convey susceptibility to neurodegeneration. While nearby GABAergic neurons are thought to be more resilient, decreased GABA signaling in other areas nonetheless correlates with age-related cognitive decline and the development of degenerative diseases. Here, we used two novel cell type-specific translating ribosome affinity purification models to elucidate the impact of healthy brain aging on the molecular profiles of dopamine and GABA neurons in the ventral midbrain. By analyzing differential gene expression from young adult (7-10 months) and old (21-24 months) mice, we detected commonalities in the aging process in both neuronal types, including increased inflammatory responses and upregulation of pro-survival pathways. Both cell types also showed downregulation of genes involved in synaptic connectivity and plasticity. Intriguingly, genes involved in serotonergic synthesis were upregulated with age in GABA neurons and not dopamine-releasing cells. In contrast, dopaminergic neurons showed alterations in genes connected with mitochondrial function and calcium signaling, which were markedly downregulated in male mice. Sex differences were detected in both neuron types, but in general were more prominent in dopamine neurons. Multiple sex effects correlated with the differential prevalence for neurodegenerative diseases such as Parkinson's and Alzheimer's seen in humans. In summary, these results provide insight into the connection between non-pathological aging and susceptibility to neurodegenerative diseases involving the ventral midbrain, and identify molecular phenotypes that could underlie homeostatic maintenance during normal aging.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Preprocessing Toolbox for 2-Photon Subcellular Calcium Imaging.","authors":"Anqi Jiang, Chong Zhao, Mark E J Sheffield","doi":"10.1523/ENEURO.0565-24.2025","DOIUrl":"10.1523/ENEURO.0565-24.2025","url":null,"abstract":"<p><p>Recording the spiking activity from subcellular compartments of neurons such as axons and dendrites during mouse behavior with 2-photon calcium imaging is increasingly common yet remains challenging due to low signal-to-noise, inaccurate region-of-interest (ROI) identification, movement artifacts, and difficulty in grouping ROIs from the same neuron. To address these issues, we present a computationally efficient preprocessing pipeline for subcellular signal detection, movement artifact identification, and ROI grouping. For subcellular signal detection, we capture the frequency profile of calcium transient dynamics by applying fast Fourier transform (FFT) on smoothed time-series calcium traces collected from axon ROIs. We then apply bandpass filtering methods (e.g., 0.05-0.12 Hz) to select ROIs that contain frequencies that match the power band of transients. To remove motion artifacts from <i>z</i>-plane movement, we apply principal component analysis on all calcium traces and use a bottom-up segmentation change-point detection model on the first principal component. After removing movement artifacts, we further identify calcium transients from noise by analyzing their prominence and duration. Finally, ROIs with high activity correlation are grouped using hierarchical or <i>k</i>-means clustering. Using axon ROIs in the CA1 region, we confirm that both clustering methods effectively determine the optimal number of clusters in pairwise correlation matrices, yielding similar groupings to \"ground truth\" data. Our approach provides a guideline for standardizing the extraction of physiological signals from subcellular compartments during rodent behavior with 2-photon calcium imaging.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Firing Activities of REM- and NREM-Preferring Neurons Are Differently Modulated by Fast Network Oscillations and Behavior in the Hippocampus, Prelimbic Cortex, and Amygdala.","authors":"Risa Kajiya, Hiroyuki Miyawaki, Hirokazu Nakahara, Kenji Mizuseki","doi":"10.1523/ENEURO.0575-24.2025","DOIUrl":"10.1523/ENEURO.0575-24.2025","url":null,"abstract":"<p><p>Sleep consists of two alternating states-rapid eye movement (REM) and non-REM (NREM) sleep. Neurons adjust their firing activity based on brain state, however, the extent to which this modulation varies across neurons and brain regions remains poorly understood. This study analyzed previously acquired 17-h continuous recordings of single-unit activity and local field potentials in the ventral hippocampal CA1 region, prelimbic cortex layer 5, and basolateral nucleus of the amygdala of fear-conditioned rats. The findings indicate that more than half of the neurons fired faster during REM sleep than during NREM sleep, although a notable subset of neurons exhibited the opposite preference, firing preferentially during NREM sleep. During sleep, the overall firing activity of both REM- and NREM-preferring neurons decreased. However, fast network oscillations, including hippocampal sharp-wave ripples (SWRs), amygdalar high-frequency oscillations, cortical ripples, and cortical spindles, differentially modulated REM- versus NREM-preferring neurons. During wakefulness, REM-preferring neurons fired more slowly but were more intensely activated by SWRs and shock presentations than NREM-preferring neurons. Moreover, during fast network oscillations in sleep, neurons with similar REM/NREM preferences exhibited stronger within- and cross-regional coactivation than those with differing preferences. Conversely, during awake SWRs in fear conditioning sessions, neurons with different REM/NREM preferences showed stronger interregional coactivation than those with similar preferences. These findings suggest that the distinct activity patterns of REM- and NREM-preferring neurons, potentially reflecting different roles in memory, affect local and global networks differently, thereby balancing experience-dependent network modifications with sleep-dependent homeostatic regulation of neuronal excitability.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Transgenic Lines Labeling Reticulospinal Neurons in Larval Zebrafish.","authors":"Elena M D Collins, Pedro T M Silva, Aaron D Ostrovsky, Sabine L Renninger, Ana R Tomás, Ruth Diez Del Corral, Michael B Orger","doi":"10.1523/ENEURO.0581-24.2025","DOIUrl":"10.1523/ENEURO.0581-24.2025","url":null,"abstract":"<p><p>From lamprey to monkeys, the organization of the descending control of locomotion is conserved across vertebrates. Reticulospinal neurons (RSNs) form a bottleneck for descending commands, receiving innervation from diencephalic and mesencephalic locomotor centers and providing locomotor drive to spinal motor circuits. Given their optical accessibility in early development, larval zebrafish offer a unique opportunity to study reticulospinal circuitry. In fish, RSNs are few, highly stereotyped, uniquely identifiable, large neurons spanning from the midbrain to the medulla. Classically labeled by tracer dye injections into the spinal cord, recent advances in genetic tools have facilitated the targeted expression of transgenes in diverse brainstem neurons of larval zebrafish. Here, we provide a comparative characterization of four existing and three newly established transgenic lines in larval zebrafish. We determine which identified neurons are consistently labeled and offer projection-specific genetic access to subpopulations of RSNs. We showcase transgenic lines that label most or all RSNs (<i>nefma, adcyap1b</i> ^{<i>ccu</i>96<i>Et</i>} ) or subsets of RSNs, including ipsilateral (<i>vsx2, calca</i> ^{<i>ccu</i>75<i>Et</i>} ), contralateral (<i>pcp4a</i> ^{<i>ccu</i>97<i>Tg</i>} ) or all (<i>tiam2a</i> ^{<i>y</i>264<i>Et</i>} ) components of the Mauthner array, or midbrain-only RSNs (<i>s1171tEt</i>). In addition to RSNs, selected transgenic lines (<i>nefma, s1171tEt, calca</i> ^{<i>ccu</i>75<i>Et</i>} ) labeled other potential neurons of interest in the brainstem. For those, we performed in situ hybridization to show expression patterns of several excitatory and inhibitory neurotransmitters at larval stages as well as glutamatergic expression patterns in juvenile fish. We provide an overview of transgene expression in the brainstem of larval zebrafish that serves to lay a foundation for future studies in the supraspinal control of locomotion.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rod inputs arrive at horizontal cell somas in mouse retina solely via rod-cone coupling.","authors":"Wallace B Thoreson, Asia L Sladek, Cody L Barta, Lou E Townsend","doi":"10.1523/ENEURO.0427-24.2025","DOIUrl":"https://doi.org/10.1523/ENEURO.0427-24.2025","url":null,"abstract":"<p><p>Rod and cone photoreceptor cells selectively contact different compartments of axon-bearing retinal horizontal cells in the mammalian retina. Cones synapse exclusively on the soma whereas rods synapse exclusively on a large axon terminal compartment. The possibility that rod signals can travel down the axon from terminal to soma has been proposed as a means of producing spectrally opponent interactions between rods and cones, but there is conflicting data about whether this actually occurs. The spectral overlap between rods and cones in mouse makes it difficult to stimulate rod and cone pigments separately. We therefore used optogenetic techniques to analyze photoreceptor inputs into horizontal somas by selectively expressing channelrhodopsin in rods and/or cones. Optogenetic stimulation of rods and cones both evoked large fast inward currents in horizontal cell somas. Cone-driven responses were abolished by eliminating synaptic release in a cone-specific knockout of the exocytotic calcium sensor, synaptotagmin 1 (Syt1). However, rod-driven responses in horizontal somas were unchanged after eliminating synaptic release from rods but abolished by eliminating release from both rods and cones. This suggests that release from cones is required for transmission of rod signals to horizontal cell somas. Rods and cones are coupled by Cx36 gap junctions and we found that selective elimination of Cx36 from rods also abolished rod-driven optogenetic responses in horizontal cell somas. Together, these results show that rod signals reach the somas of B-type horizontal cells exclusively via gap junctions with cones and not by transmission down the axon from the axon terminal.<b>Significant statement</b> Rods and cones contact different compartments of axon-bearing horizontal cells in mammalian retina: cones exclusively contact the soma whereas rods exclusively contact the axon terminal. While cone signals can traverse the axon from soma to terminal, our results show that rod signals cannot travel the other direction. The ability of rod signals to travel from terminal to soma has been proposed as a mechanism for allowing inhibitory interactions between rods and cones. This finding eliminates this pathway as an explanation for opponent rod-cone interactions in color and contrast perception.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caliber of Rohon-Beard Touch-Sensory Axons Is Dynamic In Vivo.","authors":"Kaitlin Ching, Alvaro Sagasti","doi":"10.1523/ENEURO.0043-25.2025","DOIUrl":"10.1523/ENEURO.0043-25.2025","url":null,"abstract":"<p><p>Cell shape is crucial to cell function, particularly in neurons. The cross-sectional diameter, also known as caliber, of axons and dendrites is an important parameter of neuron shape, best appreciated for its influence on the speed of action potential propagation. Many studies of axon caliber focus on cell-wide regulation and assume that caliber is static. Here, we have characterized local variation and dynamics of axon caliber in vivo using the peripheral axons of zebrafish touch-sensing neurons at embryonic stages, prior to sex determination. To obtain absolute measurements of caliber in vivo, we paired sparse membrane labeling with super-resolution microscopy of neurons in live fish. We observed that axon segments had varicose or \"pearled\" morphologies and thus vary in caliber along their length, consistent with reports from mammalian systems. Sister axon segments originating from the most proximal branch point in the axon arbor had average calibers that were uncorrelated with each other. Axon caliber also tapered across the branch point. Varicosities and caliber, overall, were dynamic on the timescale of minutes, and dynamicity changed over the course of development. By measuring the caliber of axons adjacent to dividing epithelial cells, we found that skin cell division is one aspect of the cellular microenvironment that may drive local differences and dynamics in axon caliber. Our findings support the possibility that spatial and temporal variation in axon caliber could significantly influence neuronal physiology.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleus Accumbens Dopamine Encodes the Trace Period during Appetitive Pavlovian Conditioning.","authors":"Matthew J Wanat, Brandon I Garcia-Castañeda, Cecilia Alducin-Martinez, Leonor G Cedillo, Erika T Camacho, Paul E M Phillips","doi":"10.1523/ENEURO.0016-25.2025","DOIUrl":"https://doi.org/10.1523/ENEURO.0016-25.2025","url":null,"abstract":"<p><p>Pavlovian conditioning tasks have been used to identify the neural systems involved with learning cue-outcome relationships. In delay conditioning, the conditioned stimulus (CS) overlaps or co-terminates with the unconditioned stimulus (US). Prior studies demonstrate that dopamine in the nucleus accumbens (NAc) regulates behavioral responding during delay conditioning. Furthermore, the dopamine response to the CS reflects the relative value of the upcoming reward in these tasks. In contrast to delay conditioning, trace conditioning involves a \"trace\" period separating the end of the CS and the US delivery. While dopamine has been implicated in trace conditioning, no studies have examined how NAc dopamine responds to reward-related stimuli in these tasks. Here, we developed a within-subject trace conditioning task where distinct CSs signaled either a short trace period (5 s) or a long trace period (55 s) prior to food reward delivery. Male rats exhibited greater conditioned responding and a faster response latency to the Short Trace CS relative to the Long Trace CS. Voltammetry recordings in the NAc found that the CS-evoked dopamine response increased on Short Trace trials but decreased on Long Trace trials. Conversely, US-evoked dopamine responses were greater on Long Trace trials relative to Short Trace trials. The CS dopamine response correlated with the response latency and not with conditioned responding. Furthermore, the relationship between CS dopamine and latency was best explained by an exponential function. Our results collectively illustrate that the trace period is encoded by the bidirectional NAc dopamine response to the CS during pavlovian conditioning.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":"12 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Insights into the Social Functions of the Medial Prefrontal Cortex during Infancy.","authors":"Tobias Grossmann","doi":"10.1523/ENEURO.0458-24.2025","DOIUrl":"10.1523/ENEURO.0458-24.2025","url":null,"abstract":"<p><p>The medial prefrontal cortex (mPFC) is thought to play a central role in human social perception, cognition, and behavior. In adults, the mPFC is involved in representing and interpreting the mental states in self and others. Developmental research using neuroimaging techniques like functional near-infrared spectroscopy and functional magnetic resonance imaging has begun to extend these findings into infancy. Novel evidence reviewed in this opinion demonstrates that infant mPFC (1) plays a specialized, proactive, and evaluative role in social perception, (2) is involved in connecting with other minds while interacting and when watching other minds interact, and (3) predicts overt social behavior beyond infancy. These findings suggest that, from early in human ontogeny, the mPFC plays a multifaceted role in social perception, cognition, and behavior.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":"12 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An automatic domain-general error signal is shared across tasks and predicts confidence in different sensory modalities.","authors":"Matthew J Davidson, Sriraj Aiyer, Nick Yeung","doi":"10.1523/ENEURO.0124-25.2025","DOIUrl":"https://doi.org/10.1523/ENEURO.0124-25.2025","url":null,"abstract":"<p><p>Understanding the ability to self-evaluate decisions is an active area of research. This research has primarily focused on the neural correlates of self-evaluation during visual-tasks, and whether neural correlates before or after the primary decision contribute to self-reported confidence. This focus has been useful, yet the reliance on subjective confidence reports may confound our understanding of key every-day features of metacognitive self-evaluation: that decisions must be rapidly evaluated without explicit feedback, and unfold in a multisensory world. These considerations led us to hypothesise that an automatic domain-general metacognitive signal may be shared between sensory modalities, which we tested in the present study with multivariate decoding of electroencephalographic (EEG) data. Participants (N=21, 12 female) first performed a visual task with no request for self-evaluations of performance, prior to an auditory task that included rating decision confidence on each trial. A multivariate classifier trained to predict errors in the speeded visual-task generalised to distinguish correct and error trials in the subsequent non-speeded auditory discrimination. This generalisation did not occur for classifiers trained on the visual stimulus-locked data and further predicted subjective confidence on the subsequent auditory task. This evidence of overlapping response-locked neural activity provides evidence for automatic encoding of confidence independent of any explicit request for metacognitive reports, and a shared basis for metacognitive evaluations across sensory modalities.<b>Significance Statement</b> Understanding the neural basis of self-evaluation is an important and active area of research. Here we show that neural activity following speeded responses in a visual task can predict accuracy in a later auditory judgment. This neural activity further generalised to predict confidence in the later auditory decision. This automatic encoding of self-evaluation that is shared between sensory modalities is of theoretical and practical importance, for identifying a domain-general marker of confidence that can improve our understanding of human decision making.</p>","PeriodicalId":11617,"journal":{"name":"eNeuro","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}