Electronic Journal of Biotechnology最新文献

{"title":"In silico and in vitro assessment of the anti-β-amyloid aggregation and anti-cholinesterase activities of Ptaeroxylon obliquum and Bauhinia bowkeri extracts","authors":"Michael C. Ojo ,&nbsp;Rebamang A. Mosa ,&nbsp;Foluso O. Osunsanmi ,&nbsp;Neerish Revaprasadu ,&nbsp;Andy R. Opoku","doi":"10.1016/j.ejbt.2023.11.004","DOIUrl":"10.1016/j.ejbt.2023.11.004","url":null,"abstract":"<div><h3>Background</h3><p>Alzheimer’s disease (AD) is the most common form of dementia and dementia constitutes the fifth leading cause of mortality across the globe. Available treatment modalities and drugs have abysmally failed to curtail AD. This study evaluated the mitigation of Aβ aggregation and anti-cholinesterase activities with the crude extracts of <em>Ptaeroxylon obliquum</em> and <em>Bauhinia bowkeri</em>. Computational studies of the most abundant phytochemicals from the crude extracts of both plants with proteins were investigated. The phytochemical composition of the different crude extracts (hexane, DCM, and ethanol) of the plants were analyzed with FTIR and GC-MS. The inhibitory potential of the extracts on BACE-1 and cholinesterase activities was determined with both computational molecular docking studies and <em>in vitro</em> enzyme assays. Their anti-aggregation properties were confirmed with Thioflavin-T assay and TEM.</p></div><div><h3>Results</h3><p>The <em>in silico</em> studies revealed that though thunbergol and cyclotetradecatriene (the major constituents of the extracts) inhibited all the proteins, the latter exhibited the best inhibitory potential. The <em>in vitro</em> results showed that while the dichloromethane (DCM) extract of <em>P. obliquum</em> had the highest butyrylcholinesterase (BuChE) inhibitory activity (1.77 µg/ml), the hexane and ethanol extract of <em>B. bowkeri</em> exhibited the highest β-site amyloid precursor cleaving enzymes-1 (BACE-1) (30.4 µg/ml) and acetylcholinesterase (AChE) (58.11 µg/ml) inhibitory efficacy, respectively. The ethanol extract (160 μg/ml) of <em>B. bowkeri</em> had the most efficacious anti-aggregation activity.</p></div><div><h3>Conclusions</h3><p>This study suggests that the plants could possess neuroprotective effects and could also be sources of anti-AD novel drugs.</p><p><strong>How to cite:</strong> Ojo MC, Mosa RA, Osunsanmi FO, et al. <em>In silico</em> and <em>in vitro</em> assessment of the anti-β-amyloid aggregation and anti-cholinesterase activities of <em>Ptaeroxylon obliquum</em> and <em>Bauhinia bowkeri</em> extracts. Electron J Biotechnol 2024;68. <span>https://doi.org/10.1016/j.ejbt.2023.11.004</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"68 ","pages":"Pages 67-80"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000386/pdfft?md5=456c4af6b43a6536204d52864daa620b&pid=1-s2.0-S0717345823000386-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138691320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-alcohol light beer enriched with olive leaves extract: Cold mashing technique associated with interrupted fermentation in the brewing process","authors":"Eliziane Cappelin ,&nbsp;Daiane Meneguzzi ,&nbsp;Diogo Henrique Hendges ,&nbsp;Tatiane Luiza Cadorin Oldoni ,&nbsp;Marina Leite Mitterer Daltoé ,&nbsp;Marcelo Luis Kuhn Marchioro ,&nbsp;Mario Antônio Alves da Cunha","doi":"10.1016/j.ejbt.2024.01.002","DOIUrl":"10.1016/j.ejbt.2024.01.002","url":null,"abstract":"<div><h3>Background</h3><p>Beer is the most consumed alcoholic beverage globally, and the demand for differentiated beers with peculiar characteristics has intensified among beer consumers, creating a significant market niche. In this study, we developed a low alcohol light craft beer enriched with olive leaf extract (<em>Olea europaea</em> L.). The cold mashing technique associated with interrupted fermentation was used in the mashing step. Different concentrations of olive leaf extract (0.5, 1.0 and 2.0%) were added at the maturation stage. The samples were characterized by physicochemical parameters, phenolic and polyphenolic content, bioactive compounds, antioxidant potential, and microbiological quality.</p></div><div><h3>Results</h3><p>The cold mash technique associated with interrupted fermentation provided a low-alcohol beer (≅1.3%). The bitterness dimension (19.0 to 23.2 IBU) and color (9–17 EBC) parameter were in accordance with the Beer Judge Certification Program (BJCP) for the American Blond Ale<em>-</em>style. The addition of the extract enriched the content of total phenolics (171.09 to 437.4 mg GAE/mL) and polyphenolic (221.4 to 729.0 mg/L). Coumaric, ferulic, and cinnamic phenolic acids were detected in appreciable amounts in the beers. Oleuropein was the major compound in the beverage and plant extract. After adding 2% extract, the ABTS and DPPH radical scavenging activity, as well as the ferric reduction power, increased in beers by 28.4%, 449.1%, and 120.5%, respectively.</p></div><div><h3>Conclusions</h3><p>The extract of <em>O. europaea</em> L. promoted the enrichment of low-alcohol beer samples with bioactive compounds and antioxidant potential. The results obtained indicated the potential use of <em>O. europaea</em> L. extract as a natural oxidant in other beverages and food products.</p><p><strong>How to cite:</strong> Cappelin E, Meneguzzi D, Hendges DH, et al. Low-alcohol light beer enriched with olive leaves extract: Cold mashing technique associated with interrupted fermentation in the brewing process. Electron J Biotechnol 2024; 68. <span>https://doi.org/10.1016/j.ejbt.2024.01.002</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"68 ","pages":"Pages 81-89"},"PeriodicalIF":2.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000046/pdfft?md5=2f6ef300e0d49401aba8d578671448e7&pid=1-s2.0-S0717345824000046-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139679810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNA FMN2 motivates colorectal cancer development by mediating tumor-associated macrophage polarization by controlling the microRNA-150-5p/PIK3R3 axis","authors":"YongYi Cao ,&nbsp;DeHou Cao ,&nbsp;Ting Zhu","doi":"10.1016/j.ejbt.2023.12.002","DOIUrl":"10.1016/j.ejbt.2023.12.002","url":null,"abstract":"<div><h3>Background</h3><p>It has been reported that circular RNA formin 2 (circFMN2) can motivate colorectal cancer (CRC) proliferation. However, the effect of circFMN2 on the polarization of tumor-associated macrophages (TAMs) in the CRC tumor microenvironment remains unclear. The study was to figure out the latent mechanism by which circFMN2 impacts TAM polarization to motivate the malignant behavior of CRC cells.</p></div><div><h3>Results</h3><p>circFMN2 and PIK3R3 levels were reduced in M1 macrophages but elevated in M2 macrophages, whereas miR-150-5p level was the opposite. circFMN2 knockdown downregulated M2 macrophage markers CD163, CCL22 and CD206 and upregulated M1 macrophage markers CD86, TNF-α and IL-1β in M2 macrophages. Co-culture with M2 macrophage-conditioned medium with circFMN2 knockdown reduced CRC proliferation, invasion, and migration, while knockdown of miR-150-5p had the opposite effect. CircFMN2 adsorbed miR-150-5p to mediate PIK3R3 in M2 macrophages. Overexpression of miR-150-5p can reverse the promoting effects of overexpression of circFMN2 on M2 polarization, CRC cell proliferation, invasion, and migration. Elevation of PIK3R3 could turn around the repressive effect of circFMN2 knockdown on M2 polarization and CRC cell proliferation, invasion, and migration. In an <em>in vivo</em> model, M2 macrophages expressing low or high circFMN2 were co-transplanted with CRC cells into nude mice, resulting in inhibition and promotion of tumor growth, respectively.</p></div><div><h3>Conclusions</h3><p>All in all, circFMN2 mediates TAM polarization to M2 type by controlling the miR-150-5p/PIK3R3 axis to motivate CRC development and may offer a latent molecular target for CRC treatment.</p><p><strong>How to cite:</strong> Cao Y, Cao D, Zhu T. Circular RNA FMN2 motivates colorectal cancer development by mediating tumor-associated macrophage polarization by controlling the microRNA-150-5p/PIK3R3 axis. Electron J Biotechnol 2024;68. <span>https://doi.org/10.1016/j.ejbt.2023.12.002</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"68 ","pages":"Pages 31-40"},"PeriodicalIF":2.7,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000010/pdfft?md5=06608045dd37e31b6fd81eb39e9e894d&pid=1-s2.0-S0717345824000010-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139423576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel nanoemulsion based on clove and thyme essential oils: Characterization, antibacterial, antibiofilm and anticancer activities","authors":"Amr H. Hashem ,&nbsp;Ahmed S. Doghish ,&nbsp;Ahmed Ismail ,&nbsp;Mahmoud M.H. Hassanin ,&nbsp;Mohammad K. Okla ,&nbsp;Ibrahim A. Saleh ,&nbsp;Hamada AbdElgawad ,&nbsp;Amr M. Shehabeldine","doi":"10.1016/j.ejbt.2023.12.001","DOIUrl":"10.1016/j.ejbt.2023.12.001","url":null,"abstract":"<div><h3>Background</h3><p>Essential oil nanoemulsions have received much attention in the last period due to their ability to fight microbes and cancers. In the current study, clove and thyme essential oils CL+TH-emulsion and CL+TH-nanoemulsion were prepared through an eco-friendly method. The prepared CL+TH-nanoemulsion was characterized using DLS and TEM analyses.</p></div><div><h3>Results</h3><p>Results revealed that CL+TH-nanoemulsion droplets were spherical in shape and nanoform in size (68.6 nm) with PDI 0.281. MIC concentrations of CL+TH-nanoemulsion against tested bacteria were found to be between 6.25 and 25 mg/mL. After being exposed to MICs of CL+TH-emulsion and CL+TH-nanoemulsion, which additionally prompted 1.43 log and 3.12 log declines, accordingly, as opposed to untreated (Control), the number of cells grown in the generated biofilms decreased. Furthermore, CL+TH-nanoemulsion exhibited anticancer activity more than CL+TH-emulsion toward HepG2 and MCF-7. Also, the effect of CL+TH-nanoemulsion is more effective and significantly cytotoxic than taxol on MCF-7. Besides, both prepared emulsions increased the rate of apoptosis and decreased the cell viability % of MCF-7 by increasing the activity of caspases 8 and 9. Moreover, CL+TH-nano emulsion decreased the activity of VEGFR-2 in MCF-7 in a more pronounced manner than CL+TH-emulsion and taxol.</p></div><div><h3>Conclusions</h3><p>The prepared CL+TH-nanoemulsion had antibacterial, and antibiofilm as well as anticancer properties, which can be used in different biomedical applications after extensive studies <em>in vivo</em>.</p><p><strong>How to cite:</strong> Hashem AH, Doghish AS, Ismail A, et al. A novel nanoemulsion based on clove and thyme essential oils: Characterization, antibacterial, antibiofilm and anticancer activities. Electron J Biotechnol 2024;68. <span>https://doi.org/10.1016/j.ejbt.2023.12.001</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"68 ","pages":"Pages 20-30"},"PeriodicalIF":2.7,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000404/pdfft?md5=3e579e17aa4fe26979cc943b5eb98bf7&pid=1-s2.0-S0717345823000404-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139095246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of hot spring origin bacterial cell wall polysaccharide-based copper nanoparticles with antibacterial property","authors":"Aparna Banerjee ,&nbsp;Rajendra Kr Roy ,&nbsp;Shrabana Sarkar ,&nbsp;Juan L. López ,&nbsp;Sugunakar Vuree ,&nbsp;Rajib Bandopadhyay","doi":"10.1016/j.ejbt.2023.11.005","DOIUrl":"10.1016/j.ejbt.2023.11.005","url":null,"abstract":"<div><h3>Background</h3><p>At present, research on facile, green synthesis of nanoparticles has significantly increased because of its fast, one-step, cost-effective, time-efficient, and non-toxic nature. In this study, we have reported a single-step green synthesis of copper nanoparticles using cell wall polysaccharides of a hot spring origin, thermotolerant <em>Bacillus</em> species.</p></div><div><h3>Result</h3><p>Copper nanoparticles were characterized using UV-visible spectrophotometry, fluorescence and Fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive spectroscopy, particle size, and zeta potential analyses. UV-visible spectra of synthesized copper nanoparticles exhibited a band cantered between 220–235 nm, characteristic spectra of copper oxide nanoparticles. Infrared spectra showed the band at 490-530 cm⁻¹ corresponding to metal-oxygen or copper nanoparticle vibration, supporting the presence of copper oxide nanoparticles in the monoclinic phase. The energy dispersive spectra of copper nanoparticles exhibited a strong signal from elemental copper. The dynamic Light Scattering pattern confirmed the nanoparticle nature of the studied sample. These nanoparticles showed preferential activity against gram-negative pathogens, <em>Salmonella typhi</em> and <em>Escherichia coli</em>. The thermodynamic nature of the nanoparticles is also established for its antibacterial actions.</p></div><div><h3>Conclusions</h3><p>The antibacterial action and its thermodynamics reinforce the possible use of copper nanoparticles as an alternative to commercially available antimicrobials. This study may open a new path for future studies to treat harmful microorganisms resistant to traditional antibiotics in a greener way.</p><p><strong>How to cite:</strong> Banerjee A, Roy RK, Sarkar S, et al. Synthesis of hot spring origin bacterial cell wall polysaccharide-based copper nanoparticles with antibacterial property. Electron J Biotechnol 2024;67. <span>https://doi.org/10.1016/j.ejbt.2023.11.005</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"68 ","pages":"Pages 11-19"},"PeriodicalIF":2.7,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000398/pdfft?md5=b4f16d50935278e67c835c79a1ad1a06&pid=1-s2.0-S0717345823000398-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139029287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"circUBAP2 ameliorates hypoxia-induced acute myocardial injury by competing with miR-148b-3p and mediating CDKN1B expression","authors":"FeiFei Li ,&nbsp;Li Xu ,&nbsp;JingMin Ou ,&nbsp;ZuWei Yang ,&nbsp;YuXin Dai ,&nbsp;MingKe Qiu ,&nbsp;Xin Hou ,&nbsp;DengFeng Zhu","doi":"10.1016/j.ejbt.2023.11.003","DOIUrl":"10.1016/j.ejbt.2023.11.003","url":null,"abstract":"<div><h3>Background</h3><p>A recent high-throughput sequencing study revealed an anomalous underexpression of circular RNA UBAP2 (circUBAP2) in acute myocardial infarction (AMI), yet its biological function within this context remains elusive. This study aims to unravel whether circUBAP2 is instrumental in modulating the pathogenesis of AMI and to illuminate the underlying molecular mechanisms at play.</p></div><div><h3>Results</h3><p>circUBAP2 was abnormally low expressed in AMI. Inducing circUBAP2 ameliorated hypoxia-induced myocardial cell injury by enhancing cellular viability, and decreasing lactate dehydrogenase release, apoptosis, inflammation, and oxidative damage. circUBAP2 targeted miR-148b-3p, miR-148b-3p overexpression offset circUBAP2-induced cardioprotection. Cyclin-dependent kinase inhibitor 1B (CDKN1B) was mediated by miR-148b-3p, and CDKN1B upregulation suppressed the deleterious effect of circUBAP2 silencing on hypoxic AC16 cells. In addition, overexpression of circUBAP2 improved myocardial injury, decreased myocardial cell apoptosis, and alleviated inflammation and oxidative stress in AMI mice.</p></div><div><h3>Conclusions</h3><p>circUBAP2 ameliorates AMI by competitively binding to miR-148b-3p and mediating CDKN1B expression.</p><p><strong>How to cite:</strong> Li F, Xu L, Ou J, et al. circUBAP2 ameliorates hypoxia-induced acute myocardial injury by competing with miR-148b-3p and mediating CDKN1B expression. Electron J Biotechnol 2024;67. <span>https://doi.org/10.1016/j.ejbt.2023.11.003</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"68 ","pages":"Pages 1-10"},"PeriodicalIF":2.7,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000374/pdfft?md5=c33d5a1ece8492a28112318bb08e40ef&pid=1-s2.0-S0717345823000374-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138632155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus mojavensis isolated from aguamiel and its potential as a probiotic bacterium","authors":"Verónica Miroslava Martínez-Ortiz ,&nbsp;María Alejandra Trujillo-López ,&nbsp;Elie Girgis El-Kassis ,&nbsp;Elizabeth Bautista-Rodríguez ,&nbsp;Manuel Reinhart Kirchmayr ,&nbsp;Paola Hernández-Carranza ,&nbsp;Beatriz Pérez-Armendáriz","doi":"10.1016/j.ejbt.2023.11.002","DOIUrl":"10.1016/j.ejbt.2023.11.002","url":null,"abstract":"<div><h3>Background</h3><p>Millenary fermented beverages are a source of industrially important microorganisms. Aguamiel and pulque are traditional Mexican beverages of pre-Hispanic origin, with a microbial diversity that contributes to the different fermentations (lactic, alcoholic and acetic). The aim of this research was to characterize the <em>Bacillus mojavensis</em> (BF2A1) strain isolated from aguamiel and determine its probiotic potential. The strain was identified through Mass Spectrometry (MS), molecular techniques, as well as morphological and biochemical profiling. The probiotic activity of the BF2A1 strain and its response during the gastric simulation was determined.</p></div><div><h3>Results</h3><p>The strain BF2A1 is a Gram-positive, spore-forming bacillus, positive for catalase, gamma-hemolysis, citrate, ornithine, grows at 7.5% NaCl, and acetoin, but negative for motility, indole, and methyl red. Its taxonomic identity was determined as <em>B. mojavensis</em> both by MALDI-TOF MS and sequencing of 16S rDNA. Its probiotic potential was demonstrated as BF2A1 was tolerant to pH 2 (OD_620nm 0.289 ± 0.012), 0.3% bile salts (OD_620nm 0.103 ± 0.089), 8% NaCl (OD_620nm 0.254 ± 0.096), and 1% lysozyme (OD_620nm 1.342 ± 0.078) compared to the probiotic strain <em>Lactobacillus leichmannii</em> ATCC 7830TM (Laclei). The antagonistic effect of BF2A1 against <em>Escherichia coli</em> (ATCC25922), <em>Staphylococcus aureus</em> (ATCC25923)<em>, and Candida albicans</em> (ATCC60193) showed 25.5%, 8% and, 65% inhibitory growing effect, respectively. BF2A1 in the gastric simulation showed only a reduction of 1–2 log CFU/mL and showed after the intestinal phase a survival rate of 84.4% as compared to the control strains.</p></div><div><h3>Conclusions</h3><p>This study shows that BF2A1 isolated from aguamiel is a bacterium with probiotic properties that can be used in different areas of Biotechnology.</p><p><strong>How to cite:</strong> Martínez-Ortiz V, Trujillo-López MA, El-Kassis EG, et al. <em>Bacillus mojavensis</em> isolated from aguamiel and its potential as a probiotic bacterium. Electron J Biotechnol 2024;67. <span>https://doi.org/10.1016/j.ejbt.2023.11.002</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"67 ","pages":"Pages 42-49"},"PeriodicalIF":2.7,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000362/pdfft?md5=a7fa117bd7242c28b37c68d98cb9d671&pid=1-s2.0-S0717345823000362-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-379-3p inhibits fat grafting survival and angiogenesis by targeting SOCS1-mediated adipose inflammation","authors":"JingLin Zhu ,&nbsp;FangNing Zhao ,&nbsp;XueFeng Han,&nbsp;FaCheng Li","doi":"10.1016/j.ejbt.2023.11.001","DOIUrl":"10.1016/j.ejbt.2023.11.001","url":null,"abstract":"<div><h3>Background</h3><p>This research probed the relevant mechanism of miR-379-3p by regulating suppressor of cytokine signaling1 (SOCS1) in the processes of inflammation, oxidative stress, and angiogenesis in fat grafting. An increasing body of research indicates the involvement of miRNA/mRNA pathways in the process of fat transplantation, yet the underlying molecular mechanisms remain to be fully elucidated.</p></div><div><h3>Results</h3><p>miR-379-3p knockdown improved the survival rate of adipocytes, promoted adipose tissue angiogenesis, and reduced inflammation and oxidative stress levels. miR-379-3p targeted SOCS1. SOCS1 upregulation improved adipose tissue survival and angiogenesis and reduced inflammation. miR-379-3p affected adipose tissue survival, angiogenesis, and inflammation by targeting SOCS1 expression.</p></div><div><h3>Conclusions</h3><p>miR-379-3p inhibits fat grafting survival and angiogenesis by targeting SOCS1 to mediate adipose inflammation, suffering a novel way to improve fat grafting technique development.</p><p><strong>How to cite:</strong> Zhu J, Zhao F, Han X, et al. miR-379-3p inhibits fat grafting survival and angiogenesis by targeting SOCS1-mediated adipose inflammation. Electron J Biotechnol 2024:67. <span>https://doi.org/10.1016/j.ejbt.2023.11.001</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"67 ","pages":"Pages 34-41"},"PeriodicalIF":2.7,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000350/pdfft?md5=ff7e37ffbd00eafd514916e8842f06b7&pid=1-s2.0-S0717345823000350-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis","authors":"ZhaoLian Xie,&nbsp;Hui Wang,&nbsp;LinLin Liu,&nbsp;HaiQing Zhang,&nbsp;Jing Liu","doi":"10.1016/j.ejbt.2023.10.002","DOIUrl":"10.1016/j.ejbt.2023.10.002","url":null,"abstract":"<div><h3>Background</h3><p>Glaucoma is marked by retinal neuron death in the ganglion cell layer, leading to irreversible vision loss. Aberrant long non-coding RNA (lncRNA) expression is associated with glaucoma. The study was to explore the latent molecular mechanism of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in N-methyl-D-aspartate (NMDA)-stimulated glaucoma.</p></div><div><h3>Results</h3><p>The data demonstrated that KCNQ1OT1 expression was elevated in glaucoma patients, serving as a diagnostic biomarker of glaucoma. Rats injected with NMDA developed visual loss and retinopathy and expressed high KCNQ1OT1. After treating retinal ganglion cells (RGCs) with NMDA, cell proliferation was suppressed and apoptosis was augmented. Silenced KCNQ1OT1 or HOXB3 or elevated miR-93-5p alleviated NMDA-induced suppression of RGC growth. KCNQ1OT1 mediated miR-93-5p expression by targeting homeobox box 3 (HOXB3). The protection of silenced KCNQ1OT1 in NMDA-treated RGCs was turned around by elevated HOXB3.</p></div><div><h3>Conclusions</h3><p>Overall, KCNQ1OT1 accelerates glaucoma progression via miR-93-5p/HOXB3 axis.</p><p><strong>How to cite:</strong> Xie ZL, Wang H, Liu LL, et al. Long non-coding RNA KCNQ1 opposite strand/antisense transcript 1, a potential biomarker for glaucoma, accelerates glaucoma progression via microRNA-93-5p/Homeobox box 3 axis. Electron J Biotechnol 2024;67 . <span>https://doi.org/10.1016/j.ejbt.2023.10.002</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"67 ","pages":"Pages 23-33"},"PeriodicalIF":2.7,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000349/pdfft?md5=2eb492a58f21d7b63cf1e0f5268a9b4c&pid=1-s2.0-S0717345823000349-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling SARS-CoV-2-associated lncRNAs' prognostic significance in lung adenocarcinoma-survival, immunity, and chemotherapy responses","authors":"Qianhui Zhou ,&nbsp;Ting Yuan ,&nbsp;Zhimin Xie ,&nbsp;Ying Chen","doi":"10.1016/j.ejbt.2023.10.001","DOIUrl":"https://doi.org/10.1016/j.ejbt.2023.10.001","url":null,"abstract":"<div><h3>Background</h3><p>This study investigates the link between SARS-CoV-2-associated long non-coding RNAs (lncRNAs) and lung adenocarcinoma (LUAD). LUAD is a prevalent and aggressive lung cancer type. The study aims to identify prognostic lncRNAs and construct a predictive model while shedding light on potential therapeutic targets during the COVID-19 era.</p></div><div><h3>Results</h3><p>Eight SARS-CoV-2-associated lncRNAs with significant prognostic value in LUAD were identified, forming a robust prognostic risk model. The model exhibited strong predictive performance, with high area under the ROC curve (AUC) values at one, three, and five years. Furthermore, the risk score was an independent prognostic factor, correlating with the cancer stage. Notably, differences in immune function, drug sensitivity, and immune checkpoint expression were observed between high- and low-risk groups.</p></div><div><h3>Conclusions</h3><p>This study unveils eight SARS-CoV-2-associated lncRNAs as valuable prognostic markers in LUAD, yielding a reliable prognostic risk model. Additionally, the model's ability to predict patient outcomes and its correlation with cancer stage underscores its clinical utility. The observed variances in immune function, drug sensitivity, and immune checkpoint expression suggest potential avenues for personalized LUAD treatment strategies. Clinicians can utilize the prognostic risk model to predict LUAD patient outcomes, informing treatment decisions. The insights into immune function, drug sensitivity, and immune checkpoints offer opportunities for tailored therapies, potentially enhancing patient outcomes. This study underscores the importance of considering the interplay between SARS-CoV-2-associated factors and cancer biology, especially in the context of the COVID-19 pandemic.</p><p><strong>How to cite:</strong> Zhou Q, Yuan T, Xie Z, et al. Unraveling SARS-CoV-2-associated lncRNAs' prognostic significance in lung adenocarcinoma-survival, immunity, and chemotherapy responses. Electron J Biotechnol 2024;67. <span>https://doi.org/10.1016/j.ejbt.2023.10.001</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"67 ","pages":"Pages 13-22"},"PeriodicalIF":2.7,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345823000337/pdfft?md5=f8f25c218e1eef8b2d334ae4ddc5267a&pid=1-s2.0-S0717345823000337-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134688794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}