{"title":"Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry","authors":"Oliver Birrenbach , Peter Czermak","doi":"10.1016/j.ejbt.2024.10.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>The efficiency of recombinant protein production in <em>Yarrowia lipolytica</em> is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically defined media achieve better yields and reproducibility. Defined media also increase selectivity, reduce contamination risks, and enable precise nutrient control, leading to better growth and higher productivity. We used a design of experiments approach based on the elemental composition of yeast for the arithmetic development and optimization of a defined medium for the <em>Y. lipolytica</em> auxotrophic strain PO1f.</div></div><div><h3>Results</h3><div>Statistically supported optimal concentrations of 10 g*L<sup>−1</sup> glucose and 2.29 g*L<sup>−1</sup> leucine enabled superior growth in the new <em>in silico</em> yeast (ISY) medium. Specific growth rates of 0.305 h<sup>−1</sup> were achieved for the auxotrophic <em>Yarrowia</em> strain. Thiamine hydrochloride was a growth-limiting component, and higher concentrations increased the cell density of <em>Y. lipolytica</em> PO1f cultures by a factor of 30.</div></div><div><h3>Conclusions</h3><div>ISY medium was suitable not only for the cultivation of <em>Y. lipolytica</em> but also universally applicable for <em>Yarrowia</em>-like and other yeasts, achieving better growth rates and yields compared to existing chemically defined media.</div><div><strong>How to cite:</strong> Birrenbach O, Czermak P. Development of a chemically defined medium for <em>Yarrowia</em> yeasts using a strategy of biological mimicry. Electron J Biotechnol 2025;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 32-37"},"PeriodicalIF":2.3000,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0717345824000290","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background
The efficiency of recombinant protein production in Yarrowia lipolytica is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically defined media achieve better yields and reproducibility. Defined media also increase selectivity, reduce contamination risks, and enable precise nutrient control, leading to better growth and higher productivity. We used a design of experiments approach based on the elemental composition of yeast for the arithmetic development and optimization of a defined medium for the Y. lipolytica auxotrophic strain PO1f.
Results
Statistically supported optimal concentrations of 10 g*L−1 glucose and 2.29 g*L−1 leucine enabled superior growth in the new in silico yeast (ISY) medium. Specific growth rates of 0.305 h−1 were achieved for the auxotrophic Yarrowia strain. Thiamine hydrochloride was a growth-limiting component, and higher concentrations increased the cell density of Y. lipolytica PO1f cultures by a factor of 30.
Conclusions
ISY medium was suitable not only for the cultivation of Y. lipolytica but also universally applicable for Yarrowia-like and other yeasts, achieving better growth rates and yields compared to existing chemically defined media.
How to cite: Birrenbach O, Czermak P. Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry. Electron J Biotechnol 2025;73. https://doi.org/10.1016/j.ejbt.2024.10.001.
期刊介绍:
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
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