Electronic Journal of Biotechnology最新文献

{"title":"Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry","authors":"Oliver Birrenbach ,&nbsp;Peter Czermak","doi":"10.1016/j.ejbt.2024.10.001","DOIUrl":"10.1016/j.ejbt.2024.10.001","url":null,"abstract":"<div><h3>Background</h3><div>The efficiency of recombinant protein production in <em>Yarrowia lipolytica</em> is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically defined media achieve better yields and reproducibility. Defined media also increase selectivity, reduce contamination risks, and enable precise nutrient control, leading to better growth and higher productivity. We used a design of experiments approach based on the elemental composition of yeast for the arithmetic development and optimization of a defined medium for the <em>Y. lipolytica</em> auxotrophic strain PO1f.</div></div><div><h3>Results</h3><div>Statistically supported optimal concentrations of 10 g*L⁻¹ glucose and 2.29 g*L⁻¹ leucine enabled superior growth in the new <em>in silico</em> yeast (ISY) medium. Specific growth rates of 0.305 h⁻¹ were achieved for the auxotrophic <em>Yarrowia</em> strain. Thiamine hydrochloride was a growth-limiting component, and higher concentrations increased the cell density of <em>Y. lipolytica</em> PO1f cultures by a factor of 30.</div></div><div><h3>Conclusions</h3><div>ISY medium was suitable not only for the cultivation of <em>Y. lipolytica</em> but also universally applicable for <em>Yarrowia</em>-like and other yeasts, achieving better growth rates and yields compared to existing chemically defined media.</div><div><strong>How to cite:</strong> Birrenbach O, Czermak P. Development of a chemically defined medium for <em>Yarrowia</em> yeasts using a strategy of biological mimicry. Electron J Biotechnol 2025;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 32-37"},"PeriodicalIF":2.3,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of high-value bioproducts production by marine endophytic fungus Arthrinium sp. FAKSA 10 under solid state fermentation using agro-industrial wastes","authors":"Mohammad J. Alsarraf ,&nbsp;Fuad Ameen ,&nbsp;Abdullah Alfalih ,&nbsp;Zirak Sajjad","doi":"10.1016/j.ejbt.2024.09.001","DOIUrl":"10.1016/j.ejbt.2024.09.001","url":null,"abstract":"<div><h3>Background</h3><div>To address public health challenges, there is increasing interest in bioactive metabolites from unconventional sources for targeted cancer and viral treatments with minimal side effects. Endophytic fungi are promising for these therapies. This study focused on maximizing bioactive agent yields from these fungi using a low-cost medium and optimized fermentation system.</div></div><div><h3>Results</h3><div>Forty-three endophytic fungi isolated from <em>Sinularia polydactyla</em> were cultivated on wheat bran medium and evaluated for bioactive metabolites under solid-state fermentation. Strain FAKSA 10, identified as <em>Arthrinium</em> sp. FAKSA 10, exhibited the highest levels of pharmaceutical metabolites, including L-glutaminase, L-methioninase, L-arginase, L-asparaginase, L-tyrosinase, L-lysine α-oxidase, and ribonuclease, with a 79.12% hepatitis C virus knockdown rate. This strain produced 46 metabolites with anticancer, antioxidant, antiviral, and cytotoxic properties, including major compounds like hexadecanoic acid methyl ester; hexadecanoic acid ethyl ester; 9, 12-octadecadienoic acid (Z, Z), methyl ester; 9-octadecenoic acid (Z)-, methyl ester, and 11, 14-eicosadienoic acid, methyl ester. A cost-effective strategy using 11 agro-industrial residues was applied, followed by optimization of the solid-state fermentation system. This optimization increased enzyme yields and enhanced antiviral and antioxidant activities. The optimal conditions for solid-state fermentation were a 10 d incubation, 1 mm particle size, 60% initial moisture, 28 °C temperature, and 2 × 10⁷ CFU/mL inoculum size.</div></div><div><h3>Conclusions</h3><div>This study exploited <em>Arthrinium</em> sp. FAKSA 10 metabolites as natural pharmaceuticals against cancer and viral diseases, highlighting their significant antioxidant properties. Among various residues, oil cakes emerged as an effective and cost-efficient medium, capable of significantly enhancing the production of valuable bioactive metabolites.</div><div><strong>How to cite:</strong> Alsarraf MJ, Ameen F, Alfalih A, et al. Evaluation of high-value bioproducts production by marine endophytic fungus <em>Arthrinium</em> sp. FAKSA 10 under solid state fermentation using agro-industrial wastes. Electron J Biotechnol 2024;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.09.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 1-17"},"PeriodicalIF":2.3,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic evaluation of the nanocomposites IONs-MWCNTs-Pc and IONs-GO-Pc encapsulated in the biocompatible hydrogel poly(VCL-co-PEGDA) based on photodynamic effect","authors":"Coralia Cuadrado ,&nbsp;Alex Mantilla ,&nbsp;Luis Hidalgo ,&nbsp;Diego Punina","doi":"10.1016/j.ejbt.2024.08.002","DOIUrl":"10.1016/j.ejbt.2024.08.002","url":null,"abstract":"<div><h3>Background</h3><div>The rise of drug-resistant bacteria, including <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, presents a significant healthcare challenge. This study focuses on the development of two novel nanocomposites IONs-MWCNTs-Pc and IONs-GO-Pc encapsulated within a biocompatible poly(VCL-co-PEGDA) hydrogel. These composites are designed for use in photodynamic therapy and evaluated for their antimicrobial efficacy against resistant pathogens.</div></div><div><h3>Results</h3><div>The synthesized nanocomposites, when irradiated with red light at 630 nm, showed significant antimicrobial activity, resulting in a marked reduction in the viability of <em>S. aureus</em> ATCC 27543, <em>S. aureus</em> ATCC 33591, and <em>E. coli</em> ATCC 971182. Photodynamic studies demonstrated that the IONs-GO-Pc nanocomposite was more efficient in generating singlet oxygen compared to IONs-MWCNTs-Pc, which correlated with its superior antimicrobial performance. Structural and chemical characterization confirmed the successful incorporation of nanomaterials and photosensitizers, enhancing the photodynamic effect.</div></div><div><h3>Conclusions</h3><div>The study demonstrates that both IONs-MWCNTs-Pc and IONs-GO-Pc nanocomposites show promise as alternative treatments for infections caused by antibiotic-resistant bacteria, with the GO-based composite showing higher photodynamic therapy efficacy. These findings suggest that such nanocomposites could play a pivotal role in advancing antimicrobial strategies against resistant pathogens.</div><div><strong>How to cite:</strong> Cuadrado C, Mantilla A, Hidalgo L, et al. Antibiotic evaluation of the nanocomposites IONs-MWCNTs-Pc and IONs-GO-Pc encapsulated in the biocompatible hydrogel poly(VCL-co-PEGDA) based on photodynamic effect. Electron J Biotechnol 2025;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.08.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 18-31"},"PeriodicalIF":2.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The significance of chemical transfection/transduction enhancers in promoting the viral vectors-assisted gene delivery approaches: A focus on potentials for inherited retinal diseases","authors":"Sajad Najafi ,&nbsp;Azam Rahimpour ,&nbsp;Hamid Ahmadieh ,&nbsp;Maryam Maleki Tehrani ,&nbsp;Mohammad Amin Khalilzad ,&nbsp;Fatemeh Suri ,&nbsp;Javad Ranjbari","doi":"10.1016/j.ejbt.2024.07.005","DOIUrl":"10.1016/j.ejbt.2024.07.005","url":null,"abstract":"<div><div>Viral vectors are among the main approaches currently used in studies for executing gene delivery to target cells. During the past decades of active studies in gene therapy, including viruses, adenoviruses (Ads), lentiviruses (LVs), and adeno-associated viruses (AAVs), have received the most attention among the biological approaches where potentially successful outcomes are recorded for numerous genetic conditions. The success of delivery methods, however, remains unsatisfactory. Using some additives that can improve transgene expression, transfection efficiency, viral particle production, and transduction efficiency is considered as a solution to overcoming the limitations of gene delivery approaches. These additives include caffeine, histone deacetylase (HDAC) inhibitors like sodium butyrate and valproic acid, and polycationic agents like polybrene and protamine sulfate. In this review article, we present an overview of viral vector-mediated retinal gene therapies and the application of some enhancers used to improve the outcomes of gene delivery. Three routes of administrating viral vectors into ocular compartments are employed for the delivery of target genes into impacted cells, and some additives have shown enhanced efficiency of gene delivery in retinal cells. The current study places a special focus on the viral vectors and enhancers used for gene therapies of inherited retinal diseases.</div><div><strong>How to cite:</strong> Najafi S, Rahimpour A, Ahmadieh H, et al. The significance of chemical transfection/transduction enhancers in promoting the viral vectors-assisted gene delivery approaches: A focus on potentials for inherited retinal diseases. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 29-40"},"PeriodicalIF":2.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Lactobacillus plantarum viability using novel chitosan-alginate-pectin microcapsules: Effects on gastrointestinal survival, weight management, and metabolic health","authors":"Mahla Shahriari ,&nbsp;Ashraf Mohabati Mobarez ,&nbsp;Amin Talebi Bazminabadi ,&nbsp;Masoumeh Tavakoli Yaraki","doi":"10.1016/j.ejbt.2024.07.004","DOIUrl":"10.1016/j.ejbt.2024.07.004","url":null,"abstract":"<div><h3>Background</h3><div>Probiotics, like <em>Lactobacillus plantarum</em>, show promise in managing obesity, but delivery challenges hinder their effectiveness. This study explores the effects of NCAP microcapsules with <em>L. plantarum</em> on weight, lipids, liver function, and insulin in rats over eight weeks, enhancing the bacteria’s survivability through microencapsulation.</div></div><div><h3>Results</h3><div>NCAP microcapsules (10–15 μm, &gt;79% survival) effectively protected L. plantarum. In rats, a high-fat diet with NCAP microcapsules (HFDC) significantly reduced body weight (176 g vs 179 g, <em>p</em> &lt; 0.001), low-density lipoprotein (33 mg/dL vs 44 mg/dL, <em>p</em> &lt; 0.001), and improved liver markers (aspartate transaminase 56 U/L vs 69 U/L, <em>p</em> = 0.008; alanine transaminase 36 U/L vs 38 U/L, <em>p</em> &lt; 0.001). <em>L. plantarum</em> counts were notably higher in HFDC (870,963,590 CFU/g vs 14,454 CFU/g, <em>p</em> &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>NCAP microcapsules enhance <em>L. plantarum</em> survivability and improve weight, lipids, liver function, and insulin. This study addresses the need for effective probiotic delivery systems, offering insights into metabolic health promotion.</div><div><strong>How to cite:</strong> Shahriari M, Mohabati Mobarez A, Talebi Bazminabadi A, et al. Enhancing <em>Lactobacillus plantarum</em> viability using novel chitosan-alginate-pectin microcapsules: Effects on gastrointestinal survival, weight management, and metabolic health. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 20-28"},"PeriodicalIF":2.3,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetylcholinesterase inhibition exerted by the extract of Daldinia eschscholtzii, a marine fungus associated with the coral Siderastrea siderea: GC-MS analysis and molecular docking of identified compounds","authors":"Karla G. Olivo-Flores ,&nbsp;Alan Couttolenc ,&nbsp;Jorge Suárez-Medellín ,&nbsp;Ángel Trigos ,&nbsp;César Espinoza","doi":"10.1016/j.ejbt.2024.08.001","DOIUrl":"10.1016/j.ejbt.2024.08.001","url":null,"abstract":"<div><h3>Background</h3><div>Alzheimer’s disease is a neurodegenerative disease that has no cure. The drugs used to treat Alzheimer’s disease are based on the inhibition of the enzyme acetylcholinesterase (AChE). In this sense, marine fungal metabolites represent an alternative source for the discovery of drugs based on AChE inhibition (IAChE). The present research carried out a bioprospecting study of marine fungi with acetylcholinesterase inhibition potential.</div></div><div><h3>Results</h3><div>A total of 17 marine fungi were isolated from three stony corals from the Veracruz Reef System. The fungal genera identified were <em>Geotrichum</em>, <em>Curvularia</em>, <em>Penicillium</em>, <em>Aspergillus,</em> and <em>Daldinia</em>. The IAChE was evaluated from the broth and biomass extracts of each fungal strain. As a result, <em>Daldinia eschscholtzii</em> was one of the three fungi with the greatest IAChE effect (32.7%). Subsequently, a bio-directed chromatographic purification of the methanolic extract of the biomass of <em>D. eschscholtzii</em> was carried out. The FRL8.1 fraction was the most active with an IAChE of 41.0%, a value close to Galantamine positive control (44.0%). Furthermore, compound analysis was carried out by GC-MS. Finally, it was determined that the metabolites responsible for the inhibitory effect are probably 4,7-dihydroxycoumarin and 5-nitro-2-naphthalenamine, which was demonstrated by their interactions with the AChE enzyme receptor through molecular docking studies.</div></div><div><h3>Conclusions</h3><div>Coral-associated marine fungi produce secondary metabolites that inhibit acetylcholinesterase. This is the first report of <em>D. eschscholtzii</em> isolated from the stony coral <em>Siderastrea siderea</em>. Finally, we consider that more studies are needed to demonstrate the IAChE potential of marine fungi associated with corals or other marine organisms.</div><div><strong>How to cite:</strong> Olivo-Flores KG, Couttolenc A, Suárez-Medellín J, et al. Acetylcholinesterase inhibition exerted by the extract of <em>Daldinia eschscholtzii</em>, a marine fungus associated with the coral <em>Siderastrea siderea</em>: GC–MS analysis and molecular docking of identified compounds. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.08.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 12-19"},"PeriodicalIF":2.3,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000241/pdfft?md5=a2eb14a6458060bbb9dcd2b5e6b04b8c&pid=1-s2.0-S0717345824000241-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial characteristics of endophytic Aspergillus terreus and acute oral toxicity analysis","authors":"Amr M. Shehabeldine ,&nbsp;Amer M. Abdelaziz ,&nbsp;Mostafa A. Abdel-Maksoud ,&nbsp;Mohamed A. El-Tayeb ,&nbsp;Bushra H. Kiani ,&nbsp;Ahmed S. Hussein","doi":"10.1016/j.ejbt.2024.07.003","DOIUrl":"10.1016/j.ejbt.2024.07.003","url":null,"abstract":"<div><h3>Background</h3><p>Endophytic fungi produce biologically robust metabolites suitable for diverse applications, which support the increasing exploration of these fungi. The study aimed to investigate the <em>in vitro</em> antimicrobial properties of the metabolites of endophytic fungus <em>Aspergillus terreus</em> which isolated from the leaves of <em>Psidium guajava</em> plants while the acute oral toxicity was assessed <em>in vivo.</em></p></div><div><h3>Results</h3><p><em>A. terreus</em> (OR125572) was successfully isolated for the first time from <em>P. guajava</em>. The ethyl acetate extract of <em>A. terreus</em> exhibited antibactericidal effects against <em>Enterobacter aerogenes</em> with a minimum inhibitory concentration of 18.75 mg/ml in contrast to 75.00 ± 0.0, 37.50, and 37.50 mg/ml, respectively against <em>Staphylococcus aureus, Escherichia coli, and Bacillus sphaericus</em> were 75.00 ± 0.0, 37.50, and 37.50 mg/ml, respectively. Analysis of the crude extract obtained from <em>A. terreus</em> by GC-MS revealed a total of 32 distinct compounds. The major components included 1,2-benzenedicarboxylic acid, di-iso-octyl ester; hexyl oxecan-2-one and phenol. The acute oral toxicity study found no symptoms of toxicity and no mortality until the 14th d, suggesting that the LD₅₀ value of <em>A. terreus</em> extract might exceed 1 ml/kg. The group receiving 0.5 ml/kg of <em>A. terreus</em> extract experienced a 42.9% increase in body weight, while administration of varying dosages resulted in a significant reduction in MCV levels. There was also a significant increase in the proportion of monocytes across all treated groups.</p></div><div><h3>Conclusions</h3><p>The study demonstrated the potential of <em>A. terreus</em> as a source of antimicrobial and other bioactive compounds, with relatively low acute toxicity.</p><p><strong>How to cite:</strong> Shehabeldine AM, Abdelaziz AM, Abdel-Maksoud MA, et al. Antimicrobial characteristics of endophytic <em>Aspergillus terreus</em> and acute oral toxicity analysis. Electron J Biotechnol 2024;72. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.003</span><svg><path></path></svg></span>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"72 ","pages":"Pages 1-11"},"PeriodicalIF":2.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S071734582400023X/pdfft?md5=028fec87a343f02b51c4d37f03724724&pid=1-s2.0-S071734582400023X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142130032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and analysis of candidate genes conferring alkalinity tolerance in rice (Oryza sativa L.) at the bud burst stage based on QTL-seq and RNA-seq","authors":"Jiangxu Wang ,&nbsp;Jingyang Bian ,&nbsp;Linshuai Liu ,&nbsp;Shiwei Gao ,&nbsp;Qing Liu ,&nbsp;Yanjiang Feng ,&nbsp;Lili Shan ,&nbsp;Junxiang Guo ,&nbsp;Guiling Wang ,&nbsp;Shichen Sun ,&nbsp;Hui Jiang ,&nbsp;Lei Chen ,&nbsp;Lei Lei ,&nbsp;Kai Liu","doi":"10.1016/j.ejbt.2024.07.002","DOIUrl":"10.1016/j.ejbt.2024.07.002","url":null,"abstract":"<div><h3>Background</h3><p>Soil salinization is one of the key factors restricting the production of cropland. Once rice is subjected to alkali stress at the bud burst stage, the yield will suffer irreparable serious loss. Compared with salt tolerance, studies on QTL mapping and candidate gene analysis of rice alkali tolerance are limited.</p></div><div><h3>Results</h3><p>In this study, we used the F_2:3 population derived from the alkali-tolerant cultivar LD21 and the alkali-sensitive cultivar WL138 to construct an alkali-tolerant DNA mixing pool, and the BSA (Bulked Segregation Analysis) method was used for re-sequencing. The main QTL <em>qRSLB9</em> controlling the relative shoot length of rice under alkali stress was mapped by QTL-seq. The candidate interval was narrowed to 346.5 kb by regional linkage mapping, which containing 6 DEGs screened through transcriptome sequencing. The qRT-PCR and candidate gene sequencing showed that <em>LOC_Os09g24260</em> was most likely to control relative shoot length (RSL) in rice as a major gene who encodes the WD domain, G-beta repeat domain-containing protein.</p></div><div><h3>Conclusions</h3><p>Based on these results, <em>LOC_Os09g24260</em> was the candidate gene of <em>qRSLB9</em> conferring alkalinity tolerance to rice at the bud burst stage. Our study provides valuable genetic information and materials for breeding new rice varieties with alkalinity tolerance.</p><p><strong>How to cite:</strong> Wang J, Bian J, Liu L, et al. Screening and analysis of candidate genes conferring alkalinity tolerance in rice (<em>Oryza sativa</em> L.) at the bud burst stage based on QTL-seq and RNA-seq. Electron J Biotechnol 2024;71. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.002</span><svg><path></path></svg></span>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"71 ","pages":"Pages 63-73"},"PeriodicalIF":2.3,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000228/pdfft?md5=bdb0e25ad953a411d6b44dec5c3b90c3&pid=1-s2.0-S0717345824000228-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141845944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trypsin inhibitors in seeds and pods of Phaseolus vulgaris/coccineus: A comparative study of shaking and ultrasonic extraction methods","authors":"Hourieh Tavakoli Hasanaklou ,&nbsp;Barbara Pipan ,&nbsp;Vladimir Meglič ,&nbsp;Nevena Nagl ,&nbsp;Lovro Sinkovič","doi":"10.1016/j.ejbt.2024.05.003","DOIUrl":"10.1016/j.ejbt.2024.05.003","url":null,"abstract":"<div><h3>Background</h3><p>Different methods for the extraction of trypsin inhibitors in beans (<em>Phaseolus</em> spp.) were investigated. Two randomised complete laboratory experiments were performed, one on the seeds and one on the pods. In the first, the seeds of common bean variety KIS Marcelijan, breeding line Ref_316 × 498 and runner bean variety Bonela were examined. In the second, the fresh pods of five common beans (three breeding lines, two varieties) were analysed. Four extraction methods were used, including ultrasonic-assisted extraction (UAE) for 15 and 30 min and shaking-assisted extraction for 60 and 180 min.</p></div><div><h3>Results</h3><p>The results showed a significant increase in trypsin inhibitor activity-related traits in UAE compared to shaking extraction, with the 15 min ultrasonic process showing better efficacy than the one with 30  min duration. In the seed experiment, the breeding line Ref_316 × 498 showed the highest Trypsin Units Inhibited (TUI) and TUI/mg sample after a 15 min UAE. In the pod experiment, the breeding line 228_4aa_ca also showed the highest TUI and TUI/mg sample after a 15 min extraction with UAE. These results underline the potential of UAE to maximise trypsin inhibitor content. In addition, remarkable correlations between TUI, TUI/mg sample and the percentage of trypsin inhibition (%TIn) were observed in both experiments.</p></div><div><h3>Conclusions</h3><p>These results provide valuable insights into the relationship between bean genetic resources, extraction methods and trypsin inhibitor content in bean pods and seeds and serve as a basis for refining extraction protocols. The study encourages further research on the practical implications of investigated protocols for breeding programmes and agricultural practices.</p><p><strong>How to cite:</strong> Tavakoli Hasanaklou H, Pipan B, Meglič V, et al. Trypsin inhibitors in seeds and pods of <em>Phaseolus vulgaris/coccineus</em>: A comparative study of shaking and ultrasonic extraction methods. Electron J Biotechnol 2024;71. <span><span>https://doi.org/10.1016/j.ejbt.2024.05.003</span><svg><path></path></svg></span>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"71 ","pages":"Pages 47-56"},"PeriodicalIF":2.3,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000186/pdfft?md5=eed912e78a06939ae0adb5512164c7a3&pid=1-s2.0-S0717345824000186-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141845800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel antioxidant additive ENTAN molecule for animal production: Evaluation at the cellular level","authors":"Pamela Olivares-Ferretti ,&nbsp;Ekaitz Maguregui ,&nbsp;Viviana Chavez ,&nbsp;Jorge Parodi","doi":"10.1016/j.ejbt.2024.07.001","DOIUrl":"10.1016/j.ejbt.2024.07.001","url":null,"abstract":"<div><h3>Background</h3><p>Using cell lines to explore the function of organic compounds is fundamental in biotechnology. Evaluating new additives intended to improve animal production is challenging due to the complexity and uncertainty of in vivo testing. This study investigated the action of a compound with antioxidant properties using cells from terrestrial (LMH cells line) and aquatic vertebrates (CHSE-214).</p></div><div><h3>Results</h3><p>The results of our study provide reassuring evidence of the compound’s safety for use in animal production. The compound demonstrated no adverse effects on cell viability, indicating its potential for safe application. Furthermore, the compound’s antioxidant properties were evident, with a 100% recovery in both cell lines when exposed to hydrogen peroxide 0.1 mM. It also effectively reduced cellular ageing caused by metabolic processes, as measured by the TBARS formation in both cell lines, from 5 MDA µM/mg protein to 2.5 MDA µM/mg protein when used at 0.05 or 0.5 g/L. Notably, this action did not increase cell membrane oxidation, further supporting its safety profile.</p></div><div><h3>Conclusions</h3><p>These findings indicate that the compound has an antioxidant effect and can be used independently or in combination with metabolic stimulants in the diets of production animals. Applying this additive and its possible synergy with other compounds could help reduce oxidative stress and improve growth in animal production. The data generated in this study provide a solid basis for designing diets incorporating this additive to observe improvements in animal production based on activity observed at the cellular level.</p><p><strong>How to cite:</strong> Olivares- Ferretti P, Maguregui E, Chavez V, et al. Novel antioxidant additive ENTAN molecule for animal production: Evaluation at the cellular level. Electron J Biotechnol 2024;71. <span><span>https://doi.org/10.1016/j.ejbt.2024.07.001</span><svg><path></path></svg></span>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"71 ","pages":"Pages 57-62"},"PeriodicalIF":2.3,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000216/pdfft?md5=4d676ad62e0d2e888e7139959573f92f&pid=1-s2.0-S0717345824000216-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141843496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}