Electronic Journal of Biotechnology最新文献

{"title":"Optimizing conditions for augmented production of amylase by Talaromyces islandicus AUMC 11391","authors":"Osama A.M. Al-Bedak ,&nbsp;Ahmed M. Moharram ,&nbsp;Fuad Ameen ,&nbsp;Steven L. Stephenson ,&nbsp;Marwa M.M. Idres ,&nbsp;Manal M. Yasser","doi":"10.1016/j.ejbt.2025.01.008","DOIUrl":"10.1016/j.ejbt.2025.01.008","url":null,"abstract":"<div><h3>Background</h3><div>To satisfy rising biotechnological needs, climate change, and depleting water supplies, amylases that can survive high temperatures, and salt concentrations must be developed. Amylases are the most important enzymes that comprise approximately 25–33% of the international enzyme market and have a great role in many industries.</div></div><div><h3>Results</h3><div>In this investigation, <em>Talaromyces islandicus</em> AUMC 11391 was used to produce amylase in submerged fermentation (SmF) in an augmented concentration of 232 ± 36 U/mL after 8 d of incubation at pH 6.0 and 30°C using sodium nitrate as a nitrogen supply. The obtained enzyme was partly purified employing 70% ammonium sulfate and then dialysis. The activity of the produced amylase exhibited reached the peak (992.14 ± 80 U/mg protein) at pH 5.0 and 55°C. Cu, Co, Fe, Ni, Ca, and Zn had an activating effect on the activity of the amylase enzyme at pH 5.0 and 55°C by 134.57, 123.1, 115.4, 109.76, 105.43, and 103.2%, respectively. The <em>K</em>_m and <em>V</em>_max were recorded as 132.1 mM and 60.6 µmol/min, respectively. <em>T. islandicus</em> AUMC 11391′s-amylase hydrolyzed the raw starch of maize, sorghum, wheat, rice, and oat at rates of 12.3, 113.7, 32.25, 34.67, and 73.6% in contrast to the soluble starch.</div></div><div><h3>Conclusions</h3><div>This enhanced α-amylase from <em>T. islandicus</em> AUMC 11391 looks to be a potential option to meet the present amylase requirements of a variety of industrial processes because of its improved production and beneficial properties.</div><div><strong>How to cite:</strong> Al - Bedak O.A.M., Moharram AM., Ameen F, et al. Optimizing conditions for augmented production of amylase by <em>Talaromyces islandicus</em> AUMC 11391. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.008</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 39-48"},"PeriodicalIF":2.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient in vitro regeneration and genetic fidelity analysis of shea tree (Vitellaria paradoxa Gaertn) using ISSR markers","authors":"Affi Jean Paul Attikora ,&nbsp;Souleymane Silué ,&nbsp;Mongomaké Kone ,&nbsp;Napkalo Silue ,&nbsp;Yves Kwibuka ,&nbsp;Saraka Didier Martial Yao ,&nbsp;Caroline De Clerck ,&nbsp;Sok Lay Him ,&nbsp;Nafan Diarrassouba ,&nbsp;Taofic Alabi ,&nbsp;Ludivine Lassois","doi":"10.1016/j.ejbt.2025.01.007","DOIUrl":"10.1016/j.ejbt.2025.01.007","url":null,"abstract":"<div><h3>Background</h3><div>Shea tree is an economically valuable tree crop in the food, cosmetic, and pharmaceutical industries due to its seed oil, known as shea butter. Rapid propagation of superior shea trees through <em>in vitro</em> culture is essential to support domestication and conservation efforts. This study aimed to establish an efficient <em>in vitro</em> propagation protocol for the regeneration of shea true-to-type plantlets. Nodal explants were cultured on half-strength Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and/or kinetin (Kin) combined with 1-naphthaleneacetic acid (NAA) for shoot induction. Rooting was tested on half- and quarter-strength MS and full- and half-strength modified MS (MS1B) media, enriched with indole-3-butyric acid (IBA) alone or combined with NAA, IAA, <em>meta</em>-topolin (mT), and putrescine. Genetic fidelity of regenerated plants was assessed using inter-simple sequence repeat (ISSR) markers.</div></div><div><h3>Results</h3><div>The results showed that MS/2 medium containing 3:1.2:1 mg/L BAP:Kin:NAA gave the best regeneration of axillary shoots. Four-week-old axillary shoots were 100% rooted on MS1B/2 medium containing 3:0.1:40 mg/mL IBA:<em>m</em>T:putrescine. Rooted plantlets were successfully acclimated in vivo. The polymorphism of the ISSR markers ranged from 50 to 87.5%, with an average of 65%, and the polymorphism information content was 0.22. For genetic fidelity assessment, 34 scorable and reproducible markers were obtained. All markers were monomorphic and identical to the mother plant.</div></div><div><h3>Conclusions</h3><div>The micropropagation protocol proposed in this study is suitable for large-scale <em>in vitro</em> regeneration of shea without genetic alteration. However, further studies are needed for the induction of multiple micros-hoots.</div><div><strong>How to cite:</strong> Attikora AJP, Silué S, Kone M, et al. Efficient <em>in vitro</em> regeneration and genetic fidelity analysis of shea tree (<em>Vitellaria paradoxa</em> Gaertn) using ISSR markers. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.007</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 28-38"},"PeriodicalIF":2.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection and validation of stable reference genes in guava (Psidium guajava L.) for reliable and consistent gene expression analysis","authors":"Sandeep Kumar ,&nbsp;Manoharan Muthukumar ,&nbsp;Anju Bajpai ,&nbsp;Amar Kant Kushwaha ,&nbsp;Israr Ahmad ,&nbsp;Yashi Bajpai ,&nbsp;Anshuman Singh ,&nbsp;Thukkaram Damodaran ,&nbsp;Mala Trivedi","doi":"10.1016/j.ejbt.2025.01.006","DOIUrl":"10.1016/j.ejbt.2025.01.006","url":null,"abstract":"<div><h3>Background</h3><div>Ideal stably expressing housekeeping gene to be used as internal control needs to be optimized for efficient gene expression analysis for accurate mRNA quantitation. In pursuit of identifying suitable reference genes for gene expression analysis in guava, a systematic study was conducted using different tissues of guava cv. Allahabad Safeda examining 10 housekeeping genes such as Actin (<em>PgACT</em>), Elongation factor 1G (<em>PgEF1G</em>), Elongation factor 2 (<em>PgEF2</em>), Tubulin (<em>PgTUB1</em>), Elongation factor 1 α (<em>PgEF1a</em>), Monensin sensitivity1 (<em>PgMON1</em>), Histone H3 (<em>PgH3</em>), RNA-binding protein 47 (<em>PgRBP47</em>), Polyubiquitin_X1 (<em>PgPOLX1</em>), and Polyubiquitin_X2 (<em>PgPOLX2</em>).</div></div><div><h3>Results</h3><div>qRT-PCR analysis showed amplification efficiencies ranging from 74.4% to 124.9%, with correlation coefficients exceeding 0.98. The stability of these genes’ expression was evaluated using six methods: GeNorm, NormFinder, BestKeeper, RefFinder, comparative delta-Ct, and Stability index in which different methods identified <em>PgRBP47</em> as least stable and indicated its unsuitability as a reference gene but showed variations in the ranking of the genes for gene stability.</div></div><div><h3>Conclusions</h3><div>Comparison of all the methods and accounting for the top 3 ranks of gene stability, three genes i.e., <em>PgTUB1</em>, <em>PgEF1a</em>, and <em>PgEF2</em> were identified as more stable housekeeping genes across different tissues of guava and could be considered as ideal reference genes for normalization in gene expression studies of guava.</div><div><strong>How to cite:</strong> Kumar S, Muthukumar M, Bajpai A, et al. Selection and validation of stable reference genes in guava (<em>Psidium guajava</em> L.) for reliable and consistent gene expression analysis. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 49-56"},"PeriodicalIF":2.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Erodium glaucophyllum (L.) Aiton growing in arid environment: Phytochemical characterization, antimicrobial, antioxidant, and anticancer potential","authors":"Amr H. Hashem ,&nbsp;Bahaa M. Badr ,&nbsp;Fathy M. Elkady ,&nbsp;Mostafa A. Abdel-Maksoud ,&nbsp;Abdulaziz Alamri ,&nbsp;Mohamed A. El-Tayeb ,&nbsp;Bushra H. Kiani ,&nbsp;Amer M. Abdelaziz","doi":"10.1016/j.ejbt.2025.01.005","DOIUrl":"10.1016/j.ejbt.2025.01.005","url":null,"abstract":"<div><h3>Background</h3><div>The misuse of antimicrobial agents resulted in a global serious health concern, namely antimicrobial resistance. Also, traditional bioactive compounds are associated with undesirable adverse effects. This study aimed to first assess the phytochemical analyses and biological activities of <em>Erodium glaucophyllum</em> (L.) Aiton from the arid region.</div></div><div><h3>Results</h3><div>The gas chromatography-mass spectroscopy analysis of <em>Erodium glaucophyllum</em> leaves crude extract revealed the presence of 36 biologically vital compounds, with 8 main compounds being identified. Docosenamide, hexadecanoic acid, thiocarbamic acid, hentriacontane, β-sitosterol, quinoline, and oleic acid were among the most significant compounds. Docosenamide was the most prevalent compound, comprising 45.3%. The phytochemical analysis revealed the presence of a diverse array of chemical compounds, such as carbohydrates, polyphenols, flavonoids, and tannins. In moderate concentrations, saponins, glycosides, quinones, proteins, and amino acids were present. Additionally, alkaloids, steroids, diterpenes, and cardiac glycosides were identified in trace amounts. Also, chlorogenic acid was the dominant with 69.14% among other phenolic compounds. The antimicrobial results of the tested extract showed promising activity against <em>Bacillus subtilis, Staphylococcus aureus, Salmonella typhimurium,</em> and <em>Pseudomonas aeruginosa</em>, with minimum inhibitory concentration of 31.25, 15.62, 15.62, and 62.5 µg/ml, respectively. Furthermore, the extract demonstrated potent antioxidant activity, with an EC50 of 51.7 µg/ml, and anticancer activity against MCF-7 malignant cell line, with an IC50 of 58.4 µg/ml.</div></div><div><h3>Conclusions</h3><div>The tested crude extract of <em>Erodium glaucophyllum</em> leaves represents a potential source of bioactive compounds that possess antimicrobial, antioxidant, and anticancer properties.</div><div><strong>How to cite:</strong> Hashem AH, Badr BM, Elkady FM, et al. Novel <em>Erodium glaucophyllum</em> (L.) Aiton growing in arid environment: Phytochemical characterization, antimicrobial, antioxidant, and anticancer potential. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 57-68"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Octoploid blueberry development for drought tolerance: A combined approach of in vitro polyploidization and somatic organogenesis","authors":"Alejandra Araujo Heraldez ,&nbsp;Susana Valdez Peñuelas ,&nbsp;Gabriela Jarpa-Tauler ,&nbsp;Aparna Banerjee ,&nbsp;Kattia Núñez-Montero ,&nbsp;Patricio Arce-Johnson ,&nbsp;Jesús L. Romero-Romero","doi":"10.1016/j.ejbt.2025.01.004","DOIUrl":"10.1016/j.ejbt.2025.01.004","url":null,"abstract":"<div><h3>Background</h3><div>The blueberry (<em>Vaccinium</em> spp.) is a fruit commercially known for its high quality and health benefits, particularly for its bioactive antioxidant compounds, which are important in the medical field. However, factors such as genotype, stage of fruit ripening and environmental conditions impact the biosynthesis of bioactive compounds in the berry, as well as their yield and cultivation costs. In Mexico, particularly in the state of Sinaloa, extreme climatic conditions limit the cultivation of blueberry and highlight the need for the development of new varieties with low chilling requirements and tolerance to drought conditions.</div></div><div><h3>Results</h3><div>Through the combined use of somatic organogenesis and <em>in vitro</em> polyploidization, genetic variability was promoted in the commercial blueberry plant variety “Biloxi”. To achieve this purpose, blueberry microcuttings were treated with colchicine (0.02%) for six hours for 2, 4, 6 and 8 consecutive days and induced to form shoots <em>in vitro</em> with Zeatin (1 mg·L⁻¹). Out of 304 generated plants, 36 showed lower stomatal density and 9 lines showed higher stomatal density. Likewise, 5 and 49 lines presented lower and larger stomatal sizes, respectively. In 9 lines, a higher chlorophyll content was found (10% to 200%) compared to the control treatment. Ploidy analysis using flow cytometry showed the successful generation of four octoploid blueberry plants.</div></div><div><h3>Conclusions</h3><div>This work successfully generated new octoploid blueberry plants. Currently, all the lines that presented histological, biochemical and/or genetic modifications are being evaluated under greenhouse conditions for fruit quality and drought tolerance.</div><div><strong>How to cite:</strong> Heraldez AA, Peñuelas SV, Jarpa-Tauler G, et al. Octoploid blueberry development for drought tolerance: A combined approach of <em>in vitro</em> polyploidization and somatic organogenesis. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 20-27"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of VvLRK10L affected the development of Arabidopsis thaliana","authors":"Lu Yang,&nbsp;Ding-Ding Zuo,&nbsp;Jia-Ling Xing,&nbsp;Da-Long Guo","doi":"10.1016/j.ejbt.2025.01.003","DOIUrl":"10.1016/j.ejbt.2025.01.003","url":null,"abstract":"<div><h3>Background</h3><div>Receptor-like kinase (RLK), as a phosphotransferase, is widely involved in plant growth and development and stress response. RLK in plants could be divided into different types according to the sequence characteristics. LRK10 kinase is a subfamily of RLKs and has been considered to be associated with plant disease resistance. LRK10 is differentially expressed during ABA-mediated grape berry ripening, showing its possibility with plant development.</div></div><div><h3>Results</h3><div>To explore the relationship between <em>VvLRK10L</em> genes and plant development, 4 <em>VvLRK10L</em> genes were cloned, and their conserved domains, subcellular localization and promoter activity were analyzed. <em>VvLRK10L-16</em> and <em>VvLRK10L-42</em> were localized to the plasma membrane. GA3 and ABA treatments enhanced their promoter activity, respectively, and the overexpression of these two genes promoted plant growth.</div></div><div><h3>Conclusions</h3><div><em>VvLRK10L-16</em> and <em>VvLRK10L-42</em> are located in the plasma membrane, which can respond to hormone signals and promote the development of <em>Arabidopsis thaliana</em>.</div><div><strong>How to cite:</strong> Yang L, Zuo D, Xing J, et al. Overexpression of <em>VvLRK10L</em> affected the development of <em>Arabidopsis thaliana</em>. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 11-19"},"PeriodicalIF":2.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient production of 6-hydroxynicotinic acid by newly isolated Pseudomonas poae","authors":"Yi Li ,&nbsp;Jiacheng Tang ,&nbsp;Kaixiang Xin ,&nbsp;Zongda Chen ,&nbsp;Lele Zhao ,&nbsp;Yifan Zhao ,&nbsp;Yinbiao Xu ,&nbsp;Pei Zhou ,&nbsp;Yang Sun ,&nbsp;Yupeng Liu ,&nbsp;Hua Li","doi":"10.1016/j.ejbt.2025.01.002","DOIUrl":"10.1016/j.ejbt.2025.01.002","url":null,"abstract":"<div><h3>Background</h3><div>Nicotinic acid dehydrogenase possesses the capability to convert nicotinic acid into 6-hydroxynicotinic acid, a compound of significant research value as a pharmaceutical intermediate. The extraction of nicotinic acid dehydrogenase is primarily performed by strains. However, the enzyme activity of the strains reported currently is relatively low, and their potential to catalyze the production of 6-hydroxynicotinic acid is insufficient to meet industrial requirements.</div></div><div><h3>Results</h3><div>Due to the revealing properties of 6-hydroxynicotinic acid, this study proposes a technique for calculating the luminescence intensity of colonies, which is based on a fluorescence spectrometer. The developed method establishes a reliable linear relationship (88.2%) between the luminescence intensity and enzyme activity. Consequently, it has been employed to screen strains that produce nicotinate dehydrogenase. This screening approach allows for the evaluation of about 500 enzyme-producing strains daily, presenting an efficient strategy for screening.</div></div><div><h3>Conclusions</h3><div>Through this approach, a novel high enzyme activity strain producing nicotinic acid dehydrogenase, <em>Pseudomonas poae</em> have been obtained, which is designated as HD530. After process optimization, it was utilized to produce 6-hydroxynicotinic acid, achieving a high yield of 155.45 g/L within 72 h, meeting the requirements for industrial production. The effectiveness and potential of this technique lie in its application for strain screening and improvement.</div><div><strong>How to cite:</strong> Li Y, Tang J, Xin K, et al. Efficient production of 6-hydroxynicotinic acid by newly isolated <em>Pseudomonas poae</em>. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 1-10"},"PeriodicalIF":2.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological methodologies: Comparative evaluation of microbial community and enhanced antibiotic susceptibility testing","authors":"Sinethemba H. Yakobi,&nbsp;Uchechukwu U. Nwodo","doi":"10.1016/j.ejbt.2025.01.001","DOIUrl":"10.1016/j.ejbt.2025.01.001","url":null,"abstract":"<div><h3>Background</h3><div>This study provides a comparative analysis of microbial community profiling and antibiotic susceptibility testing (AST) methodologies.</div></div><div><h3>Microbial community profiling</h3><div>Methods such as Shotgun Metagenomics and 16S rRNA sequencing were evaluated based on criteria including resolution, throughput, cost, and reproducibility. Shotgun Metagenomics was found to offer the highest resolution and detailed insights into microbial diversity, though at a higher cost and complexity. In contrast, 16S rRNA Sequencing provided a more cost-effective and high-throughput alternative, suitable for large-scale studies despite lower taxonomic resolution. Culturomics, while offering unique phenotypic data, showed variability in reproducibility and required more labor-intensive processes.</div></div><div><h3>Antibiotic susceptibility testing (AST)</h3><div>Traditional methods such as disk diffusion and broth microdilution were compared to emerging molecular and automated AST technologies. Traditional methods were noted for their precision in determining minimum inhibitory concentrations (MICs), crucial for guiding effective antimicrobial therapy. However, the emerging methods provided faster turnaround times and higher throughput, which are increasingly important in clinical settings focused on antimicrobial stewardship.</div></div><div><h3>Conclusions</h3><div>The study underscores the importance of selecting appropriate methodologies based on specific research or clinical needs, balancing factors such as cost, sensitivity, and throughput. The integration of multiple methodologies is recommended to overcome the limitations of individual techniques, providing a more comprehensive understanding of microbial ecosystems and resistance profiles. These findings are crucial for enhancing both research and clinical practices, particularly in the context of the global challenge posed by antimicrobial resistance.</div><div><strong>How to cite:</strong> Yakobi SH, Nwodo UU. Microbiological methodologies: Comparative evaluation of microbial community and enhanced antibiotic susceptibility testing. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 29-40"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the multifaceted bioactivities of silver nanoparticles synthesized from red algae Hypnea pannosa: Antimicrobial, antibiofilm, and insecticidal potentials","authors":"Mansour A.E. Bashar ,&nbsp;Enas M.H. Attia ,&nbsp;Alsayed E. Mekky ,&nbsp;Tharwat A. Selim ,&nbsp;Walaa M. Shaban ,&nbsp;Mohamed A.M. El-Tabakh ,&nbsp;Ammar.M. Mahmoud ,&nbsp;Mostafa A. Abdel-Maksoud ,&nbsp;Ali A. Ali ,&nbsp;Mohamed A. El-Tayeb ,&nbsp;Waleed B. Suleiman ,&nbsp;Mohamed E. El Beeh ,&nbsp;Sabiha Fatima ,&nbsp;Bushra Hafeez Kiani ,&nbsp;Nehal M. Khairy ,&nbsp;Ebrahim Saied","doi":"10.1016/j.ejbt.2024.12.001","DOIUrl":"10.1016/j.ejbt.2024.12.001","url":null,"abstract":"<div><h3>Background</h3><div>The emergence of microbial resistance to conventional antibiotics and the environmental impact of chemical pesticides necessitates the search for alternative and sustainable solutions. This study explores the utilization of the dried biomass of the red algae <em>Hypnea pannosa</em> to synthesize silver nanoparticles (AgNPs) using a green and eco-friendly method.</div></div><div><h3>Results</h3><div>The synthesized AgNPs displayed sizes ranging from 15 to 60 nm with polydispersed shapes and a face-centered cubic crystalline structure, confirmed through characterization techniques including transmission electron microscopy (TEM), dynamic light scattering (DLS), and X-ray diffraction (XRD). Stability assessments via zeta potential measurements and docking studies of oleic acid and 9,12-octadecadienoic acid against Gyrase B and GST proteins were also conducted. The nanoparticles demonstrated potent antibacterial and antibiofilm activities, particularly against <em>Staphylococcus epidermidis</em>. Both the algal extract and the AgNPs exhibited significant larvicidal and adulticidal effects against <em>Culex pipiens</em>, with the nanoparticles showing superior efficacy, indicated by lower LC50 and LC90 values. The highest larvicidal effectiveness achieved was 93.33% for algal extract at 600 mg/L and 100% for AgNPs at 100 mg/L.</div></div><div><h3>Conclusions</h3><div>This study provides a sustainable method for producing AgNPs with diverse applications in medical and mosquito control fields, highlighting their potential as effective and safe alternatives to conventional antibacterial and insecticidal agents.</div><div><strong>How to cite:</strong> Bashar MA, Attia EM, Mekky AE, et al. Exploring the multifaceted bioactivities of silver nanoparticles synthesized from red algae <em>Hypnea pannosa</em>: Antimicrobial, antibiofilm, and insecticidal potentials. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.12.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 54-72"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Viola odorata and Onosma hispidum: Evaluation of their antimicrobial, antioxidant, and hemolytic properties against multidrug-resistant microbes","authors":"Aneeta Andleeb ,&nbsp;Aneeza Khalid ,&nbsp;Sadia Khalil ,&nbsp;Hammad Arshad ,&nbsp;Saima Sadaf","doi":"10.1016/j.ejbt.2024.12.003","DOIUrl":"10.1016/j.ejbt.2024.12.003","url":null,"abstract":"<div><h3>Background</h3><div>Rapid increase in antimicrobial resistance poses a significant threat to global health, necessitating alternative strategies to combat multi-drug resistant (MDR) pathogens. Advances in nanobiotechnology may offer promising solutions to this pressing issue. This study revolves around developing an efficient method for synthesizing silver nanoparticles (AgNPs) based on aqueous extracts of <em>Viola odorata</em> leaves (VoL) and <em>Onosma hispidum</em> root barks (OhRb), traditional medicinal perennial herbs, followed by evaluating their antioxidant and antimicrobial potential against MDR pathogens.</div></div><div><h3>Results</h3><div>The synthesis of nanoparticles was completed in 15–20 min at 55–60°C using green approach. The characterized AgNPs showed significant antimicrobial activity against <em>Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae</em>, <em>Staphylococcus aureus, Streptococcus agalactiae</em>, and <em>Candida albicans</em>, with zones of inhibition ranging from 12 mm ± 0.636 to 25 mm ± 0.282. Minimum inhibitory concentration and minimum bactericidal concentrations ranged from 0.39 to 6.125 µg/mL. Additionally, the nanoparticles demonstrated significant antioxidant potential compared to the aqueous plant extracts. Importantly, the investigation into the effects of the nanoparticles on red blood cells revealed no pronounced hemolysis even at higher concentrations (300 µg/mL) of VoL-AgNPs and OhRb-AgNPs, demonstrating their biocompatibility and hemolytic safety profile.</div></div><div><h3>Conclusions</h3><div>The VoL-AgNPs and OhRb-AgNPs exhibit appreciable antimicrobial and antioxidant activities with minimal hemolysis, underscoring their potential to combat MDR pathogens, and further applicability in therapeutic settings. However, <em>in vivo</em> studies are warranted to explore their biomedical applications, such as in wound dressings and treatments for disorders caused by free radical damage.</div><div><strong>How to cite:</strong> Andleeb A, Khalid A, Khalil S, et al. Green synthesis of silver nanoparticles using <em>Viola odorata</em> and <em>Onosma hispidum</em>: Evaluation of their antimicrobial, antioxidant, and hemolytic properties against multidrug resistant microbes. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.12.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 19-28"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}