Electronic Journal of Biotechnology最新文献

{"title":"Overexpression of VvLRK10L affected the development of Arabidopsis thaliana","authors":"Lu Yang,&nbsp;Ding-Ding Zuo,&nbsp;Jia-Ling Xing,&nbsp;Da-Long Guo","doi":"10.1016/j.ejbt.2025.01.003","DOIUrl":"10.1016/j.ejbt.2025.01.003","url":null,"abstract":"<div><h3>Background</h3><div>Receptor-like kinase (RLK), as a phosphotransferase, is widely involved in plant growth and development and stress response. RLK in plants could be divided into different types according to the sequence characteristics. LRK10 kinase is a subfamily of RLKs and has been considered to be associated with plant disease resistance. LRK10 is differentially expressed during ABA-mediated grape berry ripening, showing its possibility with plant development.</div></div><div><h3>Results</h3><div>To explore the relationship between <em>VvLRK10L</em> genes and plant development, 4 <em>VvLRK10L</em> genes were cloned, and their conserved domains, subcellular localization and promoter activity were analyzed. <em>VvLRK10L-16</em> and <em>VvLRK10L-42</em> were localized to the plasma membrane. GA3 and ABA treatments enhanced their promoter activity, respectively, and the overexpression of these two genes promoted plant growth.</div></div><div><h3>Conclusions</h3><div><em>VvLRK10L-16</em> and <em>VvLRK10L-42</em> are located in the plasma membrane, which can respond to hormone signals and promote the development of <em>Arabidopsis thaliana</em>.</div><div><strong>How to cite:</strong> Yang L, Zuo D, Xing J, et al. Overexpression of <em>VvLRK10L</em> affected the development of <em>Arabidopsis thaliana</em>. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 11-19"},"PeriodicalIF":2.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient production of 6-hydroxynicotinic acid by newly isolated Pseudomonas poae","authors":"Yi Li ,&nbsp;Jiacheng Tang ,&nbsp;Kaixiang Xin ,&nbsp;Zongda Chen ,&nbsp;Lele Zhao ,&nbsp;Yifan Zhao ,&nbsp;Yinbiao Xu ,&nbsp;Pei Zhou ,&nbsp;Yang Sun ,&nbsp;Yupeng Liu ,&nbsp;Hua Li","doi":"10.1016/j.ejbt.2025.01.002","DOIUrl":"10.1016/j.ejbt.2025.01.002","url":null,"abstract":"<div><h3>Background</h3><div>Nicotinic acid dehydrogenase possesses the capability to convert nicotinic acid into 6-hydroxynicotinic acid, a compound of significant research value as a pharmaceutical intermediate. The extraction of nicotinic acid dehydrogenase is primarily performed by strains. However, the enzyme activity of the strains reported currently is relatively low, and their potential to catalyze the production of 6-hydroxynicotinic acid is insufficient to meet industrial requirements.</div></div><div><h3>Results</h3><div>Due to the revealing properties of 6-hydroxynicotinic acid, this study proposes a technique for calculating the luminescence intensity of colonies, which is based on a fluorescence spectrometer. The developed method establishes a reliable linear relationship (88.2%) between the luminescence intensity and enzyme activity. Consequently, it has been employed to screen strains that produce nicotinate dehydrogenase. This screening approach allows for the evaluation of about 500 enzyme-producing strains daily, presenting an efficient strategy for screening.</div></div><div><h3>Conclusions</h3><div>Through this approach, a novel high enzyme activity strain producing nicotinic acid dehydrogenase, <em>Pseudomonas poae</em> have been obtained, which is designated as HD530. After process optimization, it was utilized to produce 6-hydroxynicotinic acid, achieving a high yield of 155.45 g/L within 72 h, meeting the requirements for industrial production. The effectiveness and potential of this technique lie in its application for strain screening and improvement.</div><div><strong>How to cite:</strong> Li Y, Tang J, Xin K, et al. Efficient production of 6-hydroxynicotinic acid by newly isolated <em>Pseudomonas poae</em>. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 1-10"},"PeriodicalIF":2.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological methodologies: Comparative evaluation of microbial community and enhanced antibiotic susceptibility testing","authors":"Sinethemba H. Yakobi,&nbsp;Uchechukwu U. Nwodo","doi":"10.1016/j.ejbt.2025.01.001","DOIUrl":"10.1016/j.ejbt.2025.01.001","url":null,"abstract":"<div><h3>Background</h3><div>This study provides a comparative analysis of microbial community profiling and antibiotic susceptibility testing (AST) methodologies.</div></div><div><h3>Microbial community profiling</h3><div>Methods such as Shotgun Metagenomics and 16S rRNA sequencing were evaluated based on criteria including resolution, throughput, cost, and reproducibility. Shotgun Metagenomics was found to offer the highest resolution and detailed insights into microbial diversity, though at a higher cost and complexity. In contrast, 16S rRNA Sequencing provided a more cost-effective and high-throughput alternative, suitable for large-scale studies despite lower taxonomic resolution. Culturomics, while offering unique phenotypic data, showed variability in reproducibility and required more labor-intensive processes.</div></div><div><h3>Antibiotic susceptibility testing (AST)</h3><div>Traditional methods such as disk diffusion and broth microdilution were compared to emerging molecular and automated AST technologies. Traditional methods were noted for their precision in determining minimum inhibitory concentrations (MICs), crucial for guiding effective antimicrobial therapy. However, the emerging methods provided faster turnaround times and higher throughput, which are increasingly important in clinical settings focused on antimicrobial stewardship.</div></div><div><h3>Conclusions</h3><div>The study underscores the importance of selecting appropriate methodologies based on specific research or clinical needs, balancing factors such as cost, sensitivity, and throughput. The integration of multiple methodologies is recommended to overcome the limitations of individual techniques, providing a more comprehensive understanding of microbial ecosystems and resistance profiles. These findings are crucial for enhancing both research and clinical practices, particularly in the context of the global challenge posed by antimicrobial resistance.</div><div><strong>How to cite:</strong> Yakobi SH, Nwodo UU. Microbiological methodologies: Comparative evaluation of microbial community and enhanced antibiotic susceptibility testing. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 29-40"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the multifaceted bioactivities of silver nanoparticles synthesized from red algae Hypnea pannosa: Antimicrobial, antibiofilm, and insecticidal potentials","authors":"Mansour A.E. Bashar ,&nbsp;Enas M.H. Attia ,&nbsp;Alsayed E. Mekky ,&nbsp;Tharwat A. Selim ,&nbsp;Walaa M. Shaban ,&nbsp;Mohamed A.M. El-Tabakh ,&nbsp;Ammar.M. Mahmoud ,&nbsp;Mostafa A. Abdel-Maksoud ,&nbsp;Ali A. Ali ,&nbsp;Mohamed A. El-Tayeb ,&nbsp;Waleed B. Suleiman ,&nbsp;Mohamed E. El Beeh ,&nbsp;Sabiha Fatima ,&nbsp;Bushra Hafeez Kiani ,&nbsp;Nehal M. Khairy ,&nbsp;Ebrahim Saied","doi":"10.1016/j.ejbt.2024.12.001","DOIUrl":"10.1016/j.ejbt.2024.12.001","url":null,"abstract":"<div><h3>Background</h3><div>The emergence of microbial resistance to conventional antibiotics and the environmental impact of chemical pesticides necessitates the search for alternative and sustainable solutions. This study explores the utilization of the dried biomass of the red algae <em>Hypnea pannosa</em> to synthesize silver nanoparticles (AgNPs) using a green and eco-friendly method.</div></div><div><h3>Results</h3><div>The synthesized AgNPs displayed sizes ranging from 15 to 60 nm with polydispersed shapes and a face-centered cubic crystalline structure, confirmed through characterization techniques including transmission electron microscopy (TEM), dynamic light scattering (DLS), and X-ray diffraction (XRD). Stability assessments via zeta potential measurements and docking studies of oleic acid and 9,12-octadecadienoic acid against Gyrase B and GST proteins were also conducted. The nanoparticles demonstrated potent antibacterial and antibiofilm activities, particularly against <em>Staphylococcus epidermidis</em>. Both the algal extract and the AgNPs exhibited significant larvicidal and adulticidal effects against <em>Culex pipiens</em>, with the nanoparticles showing superior efficacy, indicated by lower LC50 and LC90 values. The highest larvicidal effectiveness achieved was 93.33% for algal extract at 600 mg/L and 100% for AgNPs at 100 mg/L.</div></div><div><h3>Conclusions</h3><div>This study provides a sustainable method for producing AgNPs with diverse applications in medical and mosquito control fields, highlighting their potential as effective and safe alternatives to conventional antibacterial and insecticidal agents.</div><div><strong>How to cite:</strong> Bashar MA, Attia EM, Mekky AE, et al. Exploring the multifaceted bioactivities of silver nanoparticles synthesized from red algae <em>Hypnea pannosa</em>: Antimicrobial, antibiofilm, and insecticidal potentials. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.12.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 54-72"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Viola odorata and Onosma hispidum: Evaluation of their antimicrobial, antioxidant, and hemolytic properties against multidrug-resistant microbes","authors":"Aneeta Andleeb ,&nbsp;Aneeza Khalid ,&nbsp;Sadia Khalil ,&nbsp;Hammad Arshad ,&nbsp;Saima Sadaf","doi":"10.1016/j.ejbt.2024.12.003","DOIUrl":"10.1016/j.ejbt.2024.12.003","url":null,"abstract":"<div><h3>Background</h3><div>Rapid increase in antimicrobial resistance poses a significant threat to global health, necessitating alternative strategies to combat multi-drug resistant (MDR) pathogens. Advances in nanobiotechnology may offer promising solutions to this pressing issue. This study revolves around developing an efficient method for synthesizing silver nanoparticles (AgNPs) based on aqueous extracts of <em>Viola odorata</em> leaves (VoL) and <em>Onosma hispidum</em> root barks (OhRb), traditional medicinal perennial herbs, followed by evaluating their antioxidant and antimicrobial potential against MDR pathogens.</div></div><div><h3>Results</h3><div>The synthesis of nanoparticles was completed in 15–20 min at 55–60°C using green approach. The characterized AgNPs showed significant antimicrobial activity against <em>Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae</em>, <em>Staphylococcus aureus, Streptococcus agalactiae</em>, and <em>Candida albicans</em>, with zones of inhibition ranging from 12 mm ± 0.636 to 25 mm ± 0.282. Minimum inhibitory concentration and minimum bactericidal concentrations ranged from 0.39 to 6.125 µg/mL. Additionally, the nanoparticles demonstrated significant antioxidant potential compared to the aqueous plant extracts. Importantly, the investigation into the effects of the nanoparticles on red blood cells revealed no pronounced hemolysis even at higher concentrations (300 µg/mL) of VoL-AgNPs and OhRb-AgNPs, demonstrating their biocompatibility and hemolytic safety profile.</div></div><div><h3>Conclusions</h3><div>The VoL-AgNPs and OhRb-AgNPs exhibit appreciable antimicrobial and antioxidant activities with minimal hemolysis, underscoring their potential to combat MDR pathogens, and further applicability in therapeutic settings. However, <em>in vivo</em> studies are warranted to explore their biomedical applications, such as in wound dressings and treatments for disorders caused by free radical damage.</div><div><strong>How to cite:</strong> Andleeb A, Khalid A, Khalil S, et al. Green synthesis of silver nanoparticles using <em>Viola odorata</em> and <em>Onosma hispidum</em>: Evaluation of their antimicrobial, antioxidant, and hemolytic properties against multidrug resistant microbes. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.12.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 19-28"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant, and enzyme inhibition effects of chia (Salvia hispanica) seed oil: A comprehensive phytochemical screening using LC-HR/MS","authors":"Muzaffer Mutlu ,&nbsp;Zeynebe Bingol ,&nbsp;Eda Mehtap Ozden ,&nbsp;Ekrem Köksal ,&nbsp;Adem Erturk ,&nbsp;Ahmet C. Goren ,&nbsp;Saleh Alwasel ,&nbsp;İlhami Gulcin","doi":"10.1016/j.ejbt.2024.12.002","DOIUrl":"10.1016/j.ejbt.2024.12.002","url":null,"abstract":"<div><h3>Background</h3><div>In this study, the antioxidant and anti-Alzheimer's disease properties of Chia (<em>Salvia hispanica</em>) seed oil (CSO) were determined for the first time. Three different metal reduction and two different radical scavenging methods were used to determine the antioxidant properties of CSO. It exhibited higher antioxidant activity than vitamins E and C in the CUPRAC method.</div></div><div><h3>Results</h3><div>CSO has shown excellent efficacy in the antioxidant methods used in this study. In the DPPH radical scavenging method, CSO exhibited higher radical scavenging potential than BHT, a standard and synthetic antioxidant. The anti-Alzheimer's disease properties of CSO were determined by inhibition of acetylcholinesterase enzyme and its IC₅₀ value (17.60 µg/mL) was found to be close to the IC₅₀ value of tacrine (8.82 µg/mL), the standard inhibitor of the enzyme. Inhibition properties of α-glycosidase enzyme and human carbonic anhydrase II isoenzymes were also studied. It was understood that CSO inhibited both enzymes at a lower rate than standard inhibitors. Also, the total phenolic and flavonoid contents of CSO were determined as 784.44 µg gallic acid equivalent (GAE)/mL oil and quercetin 150.00 µg QE)/mL oil, respectively. In addition, LC-HRMS chromatography application was performed to understand the phenolic content of CSO. It was determined that isosakuranetin (29.07 mg/L oil) was the most abundant polyphenolic compound in CSA. Also, seven polyphenolics of the studied remained below the detectable amount.</div></div><div><h3>Conclusions</h3><div>It was found that CSO had effective antioxidant activity, polyphenolic contents and potent enzyme inhibition properties, associated with some global disease.</div><div><strong>How to cite:</strong> Mutlu M, Bingol Z, Ozden EM, et al. Antioxidant, and enzyme inhibition effects of chia (<em>Salvia hispanica</em>) seed oil: A comprehensive phytochemical screening using LC-HR/MS. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.12.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 41-53"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dr. Rodolfo Quintero Ramírez (21/07/1947–08/12/2024)","authors":"Agustin López Munguía","doi":"10.1016/j.ejbt.2025.03.001","DOIUrl":"10.1016/j.ejbt.2025.03.001","url":null,"abstract":"","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 73-74"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress","authors":"Yaqian Liu,&nbsp;Yonggang Sun,&nbsp;Yincang Han,&nbsp;Weiqin Ding,&nbsp;Shengwei Jin,&nbsp;Jianyu Chen,&nbsp;Fajie Gou","doi":"10.1016/j.ejbt.2024.10.004","DOIUrl":"10.1016/j.ejbt.2024.10.004","url":null,"abstract":"<div><h3>Background</h3><div>The aim of this study was to screen circRNAs related to fat deposition in yaks, and to identify candidate circRNAs for yak meat quality improvement. Six male yaks with insignificant differences in body weights were selected as test subjects, and 3 yaks (G18_IMF) were randomly slaughtered at the beginning of the experiment, while the remaining 3 yaks were naturally grazed until 24 months of age (G24_IMF), and then slaughtered at the end of the experiment, and the intramuscular fat was collected from the dorsal muscle. At the end of the experiment, the yaks were slaughtered and the intramuscular fat from the back was collected for whole transcriptome sequencing.</div></div><div><h3>Results</h3><div>The results showed that 352 differential circRNAs, 86 differential miRNAs and 3981 differential mRNAs were found. miRNAs and mRNAs network regulation maps were successfully constructed through gene expression correlation analysis and target gene prediction.</div></div><div><h3>Conclusions</h3><div>Taking the intersection of the predicted circRNA target genes with the differential miRNAs for intramuscular fat in yaks of different months of age, we obtained two candidate ceRNA pairs that might be related to intramuscular fat deposition in yaks, and found that bta-let-7i might be related to fat deposition in yaks and might be regulated by X_85287959_85291068, and that X_85287959_85291068 could be a candidate circRNA to enhance the quality of yak meat. The results may provide a reference for further investigation of the regulatory network of intramuscular fat deposition in yak.</div><div><strong>How to cite:</strong> Liu Y, Sun Y, Han Y, et al. Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 11-18"},"PeriodicalIF":2.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143284229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology and experimental verification reveal the mechanism of Qingfei Tongluo mixture in treating pulmonary fibrosis","authors":"Ying Zhou ,&nbsp;Wenlong Wang ,&nbsp;Wanping Zhu ,&nbsp;Tingting Cai ,&nbsp;Nannan Wang ,&nbsp;Xia Liu ,&nbsp;Wenmin Wang ,&nbsp;Kequn Chai","doi":"10.1016/j.ejbt.2024.10.002","DOIUrl":"10.1016/j.ejbt.2024.10.002","url":null,"abstract":"<div><h3>Background</h3><div>Pulmonary fibrosis (PF) is a chronic interstitial lung disease posing significant health risks. This study aimed to investigate the therapeutic mechanism of Qingfei Tongluo mixture (QTm) in treating PF by combining network pharmacology and experimental verification.</div></div><div><h3>Results</h3><div>A total of 246 active ingredients in QTm were identified, with 159 potential targets for PF treatment. Quercetin, a key active ingredient, was associated with the TGF-β1 signaling pathway. Gene Ontology and KEGG enrichment analyses identified 42 core genes, with a notable implication of the TGF-beta signaling pathway in PF. Immunohistochemistry showed elevated FTO and TGF-β1 levels in PF tissues. Animal experiments demonstrated that QTm improved alveolar structure, reduced interstitial lesions, and enhanced lung function while decreasing hydroxyproline content and the expression of FTO and TGF-β1 proteins.</div></div><div><h3>Conclusions</h3><div>QTm may inhibit PF progression by suppressing FTO/TGF-β1 expression, thereby improving lung function. These findings suggest that QTm holds potential as a treatment for PF.</div><div><strong>How to cite:</strong> Zhou Y, Wang W, Zhu W, et al. Network pharmacology and experimental verification reveal the mechanism of Qingfei Tongluo mixture in treating pulmonary fibrosis. Electron J Biotechnol 2025;73. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"73 ","pages":"Pages 38-47"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sustainable exploitation of high-protein feather waste for production of cold-adapted keratinase by Penicillium lanosocoeruleum KSA-55","authors":"Eman Alhomaidi","doi":"10.1016/j.ejbt.2024.10.003","DOIUrl":"10.1016/j.ejbt.2024.10.003","url":null,"abstract":"<div><h3>Background</h3><div>The synthesis of keratinases by psychrophilic and psychrotolerant microorganisms has not received much attention, despite the fact that they can be an effective stand-in for substrate conversion at a low energy cost.</div></div><div><h3>Results</h3><div>In this study, some psychrophilic fungi isolated from three cold storage locations near Riyadh, Saudi Arabia, were tested for their cold-active keratinase potential. <em>Penicillium lanosocoeruleum</em>, a recently isolated fungus from Saudi Arabia, was the potent strain that produced cold-active keratinase. The <em>Penicillium</em> species was identified using sequencing of the internal transcribed spacer region (ITS). The generation of cold-active keratinase by <em>P. lanosocoeruleum</em> KSA-55 was optimized by two factors at time (TFAT). At pH 9.0 and 15°C, the keratinase activity was 28.9 ± 2.8 U/mL/min which increased to 41.7 ± 3.8 U/mL/min after 6 d of fermentation using peptone as a nitrogen source. The produced keratinase was chromatographed by MP 800 anion exchange resin and Sephadex G 100 size exclusion gel. At pH 9.0 and 10°C, the pure keratinase displayed the maximum specific activity of 210.3 ± 8.4 U/mg. Zn²⁺, Fe²⁺, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and phenylmethanesulfonyl fluoride (PMSF), demonstrated severe inhibitory effects on the keratinase activity. Mn²⁺ ions activated the keratinase by 166.85 ± 15.6%. PMSF significantly reduced keratinase activity.</div></div><div><h3>Conclusions</h3><div><em>P. lanosocoeruleum</em> strain KSA-55 is presented here as a new prospective producer of cold-active keratinase for a variety of biotechnological uses, including the management of keratinous waste in the poultry industry, cosmetics, and medical applications.</div><div><strong>How to cite:</strong> Alhomaidi E. A sustainable exploitation of high-protein feather waste for production of cold-adapted keratinase by <em>Penicillium lanosocoeruleum</em> KSA-55. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.10.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 1-10"},"PeriodicalIF":2.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143169491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}