Electronic Journal of Biotechnology最新文献

{"title":"Obituary for James D. Watson (1928–2025)","authors":"Graciela Muñoz-Riveros","doi":"10.1016/j.ejbt.2025.100699","DOIUrl":"10.1016/j.ejbt.2025.100699","url":null,"abstract":"","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100699"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic synthesis of silver nanoparticles using cell-free extracts of thermotolerant bacteria: Antioxidant and antibacterial properties","authors":"Aparna Banerjee ,&nbsp;Ismael Herrera-Vargas ,&nbsp;Mario E. Flores ,&nbsp;Francisca Valenzuela ,&nbsp;Srijan Banerjee","doi":"10.1016/j.ejbt.2025.100698","DOIUrl":"10.1016/j.ejbt.2025.100698","url":null,"abstract":"<div><h3>Background</h3><div>Eco-friendly synthesis of silver nanoparticles (AgNPs) using biological systems offers a sustainable alternative to conventional physicochemical methods. In this study, we employed cell-free extracts from three thermotolerant bacterial strains, <em>Bacillus haynesii</em> CamB6, <em>Pseudomonas alcaligenes</em> Med1, and <em>Staphylococcus</em> sp. BSP3 for the biosynthesis of AgNPs, aiming to explore their antioxidant and antibacterial properties.</div></div><div><h3>Results</h3><div>The biosynthesized AgNPs were characterized through UV–Vis spectroscopy, FTIR, TEM, and DLS analyses, which revealed distinct physicochemical profiles among the nanoparticles. Notably, AgNP2 and AgNP3 exhibited smaller particle sizes, enhanced colloidal stability, and superior biological activities compared to AgNP1. Antioxidant evaluation demonstrated significant free radical scavenging potential, with AgNP2 showing the highest DPPH activity (65.18% at 5 mg mL⁻¹). Antibacterial activity, assessed via agar well diffusion and cell viability assays against <em>Bacillus cereus</em> and <em>Pseudomonas putida</em> revealed that AgNP2 achieved the lowest bacterial viability (0.74%) for <em>P. putida</em> at 1 mg mL⁻¹ concentration.</div></div><div><h3>Conclusions</h3><div>The study highlights the potential of biosynthesized AgNPs, particularly AgNP2, as sustainable for biomedical applications. Their antioxidant and antibacterial activities suggest valuable applications in managing oxidative stress and combating antimicrobial resistance.</div><div><strong>How to cite:</strong> Banerjee A, Herrera-Vargas I, Flores ME, et al. Biogenic synthesis of silver nanoparticles using cell-free extracts of thermotolerant bacteria: Antioxidant and antibacterial properties. Electron J Biotechnol 2026:79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100698</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100698"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anoikis- and m6A-related lncRNA analysis to identify prognostic indicators in liver hepatocellular carcinoma","authors":"Pan Yu ,&nbsp;Shuaiyang Jing ,&nbsp;Sarinder Kaur Dhillon","doi":"10.1016/j.ejbt.2025.100701","DOIUrl":"10.1016/j.ejbt.2025.100701","url":null,"abstract":"<div><h3>Background</h3><div>In cancer, the process of anoikis is intimately associated with the emergence and progression. N6-methyladenosine modification and m6A modification play an important role in regulating long non-coding RNAs. The liver hepatocellular carcinoma patients’ data, including clinical and prognostic data, were obtained via The Cancer Genome Atlas database. The univariate, multivariate Cox and Least Absolute Selection Operator (LASSO) regression were performed to gain anoikis- and m6A-related lncRNAs. The Kaplan-Meier method was employed to assess the overall survival rate for groups of high- and low risks.</div></div><div><h3>Results</h3><div>A signature comprising six anoikis- and m6A-related lncRNAs was constructed: AL117336.3, LINC01138, Z83851.1, NRAV, CASC19 and AC009283.1. The clinicopathological variables, the anoikis- and m6A-related lncRNA signature demonstrated superior diagnostic efficacy, with an area under the receiver operating characteristic curve of 0.810. In the high-risk group, the overall survival was shown to be inferior to that of in group of low risk, while patients were classified by distinct clinicopathological variables. The ssGSEA and CIBERSORT immune analysis demonstrated that the predictive signature was significantly associated with liver cancer patients’ immune status. The chemotherapy drugs ATRA, AUY922, bexarotene, gemcitabine, mitomycin-C, and PHA have been found to have greater sensitivity in treating high-risk patients. qRT-PCR showed that Z83851.1, NRAV and CASC19 lncRNAs were associated with poor prognosis and were high-risk factors. AC009283.1 lncRNA may have anti-cancer properties.</div></div><div><h3>Conclusions</h3><div>The predictive signature is capable of independently predicting the prognosis of liver cancer patients for understanding the mechanisms of anoikis- and m6A-related lncRNAs in liver hepatocellular carcinoma and offering clinical guidance to patients with liver cancer.</div><div><strong>How to cite:</strong> Yu P, Jing S, Dhillon SK. Anoikis and m6A related lncRNAs analysis to identify prognostic indicators in liver hepatocellular carcinoma. Electron J Biotechnol 2026;79. <span><span>https://doi.org/10.1016/j.ejbt.2025.100701</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"79 ","pages":"Article 100701"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the genomic position of xylanase genes on Bacillus safensis FB03 and optimizing the xylanase fermentation medium by Box-Behnken Design from an unconventional carbon source","authors":"Farhana Boby ,&nbsp;Md Nurul Huda Bhuiyan ,&nbsp;Md Mashud Parvez ,&nbsp;Md Jahidul Islam ,&nbsp;Ifrat Jannati","doi":"10.1016/j.ejbt.2025.05.004","DOIUrl":"10.1016/j.ejbt.2025.05.004","url":null,"abstract":"<div><h3>Background</h3><div>The ability of <em>Bacillus safensis</em> to synthesize xylanase and other industrially important enzymes utilizing lignocellulosic biomass makes it advantageous for a variety of biotechnology applications. Thus, the current investigation aimed to optimize conditions and medium components for maximizing xylanase production by a newly isolated <em>Bacillus safensis</em> strain using banana rachis (peel of banana tree) as a novel source of carbon.</div></div><div><h3>Result</h3><div>Upon employing Box-Behnken Design (BBD) statistical approach, the highest enzyme activity was obtained 25.24 U/ml at 2 g/L banana rachis, 1 g/L yeast extract, 1 g/L K₂HPO₄, 5 g/L NaNO₃, 35°C and 72 h of incubation time. The purified enzyme showed 10 times higher enzyme activity (143.6 U/ml) with 2.3 mg/ml protein concentration. The enzyme was found to maintain stability up to 60°C in a wide range of pH (6 to 10). Analysis of whole genome sequencing data revealed the presence of xylanase production and xylan metabolic genes (xynA, xynB, xylP, xylT) on <em>Bacillus safensis</em> FB03. Also, from genome annotation, different carbohydrate metabolic genes such as glycoside hydrolases (GHs), glycosyl transferases (GTs), polysaccharide lyases (PLs), carbohydrate esterases (CEs), auxiliary activities (AAs), and carbohydrate binding modules (CBMs) were identified.</div></div><div><h3>Conclusions</h3><div>In accordance with our research, banana rachis can be considered as a major medium component to develop an economical fermentation process for the production of xylanase by <em>Bacillus safensis</em> FB03. Additionally, identification of the genomic location of xyl genes provides valuable insight towards genetic engineering for the development of a more potent industrial strain.</div><div><strong>How to cite:</strong> Boby F, Huda Bhuiyan MN, Parvez MM, et al. Mapping the genomic position of xylanase genes on <em>Bacillus safensis</em> FB03 and optimizing the xylanase fermentation medium by Box-Behnken Design from an unconventional carbon source. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 76-85"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALYREF promotes malignant behaviors and inhibits ferroptosis in colon cancer cells by stabilizing PCSK9 mRNA","authors":"Lili Cao ,&nbsp;Ying Chen ,&nbsp;Jing Yu ,&nbsp;Dian Yin","doi":"10.1016/j.ejbt.2025.07.003","DOIUrl":"10.1016/j.ejbt.2025.07.003","url":null,"abstract":"<div><h3>Background</h3><div>Colon cancer is a prevalent malignancy causing significant global morbidity and mortality. The RNA methyltransferase Aly/REF export factor (ALYREF), which binds 5-methylcytosine (m5C)-modified messenger RNA, represents a potential diagnostic and therapeutic target in cancer. However, its specific role and mechanism in colon cancer progression remain unexplored.</div></div><div><h3>Results</h3><div>ALYREF expression was significantly elevated in colon cancer tissues and cell lines compared to normal controls. Depletion of ALYREF suppressed colon cancer cell proliferation, migration, and invasion, while simultaneously promoting apoptosis and ferroptosis. Analysis revealed proprotein convertase subtilisin/kexin type 9 (PCSK9) is highly expressed in colon cancer and positively regulated by ALYREF. Mechanistically, ALYREF directly bound to and stabilized PCSK9 messenger RNA in a manner dependent on m5C modification. Crucially, the anti-tumor effects resulting from ALYREF knockdown were reversed by overexpressing PCSK9. Consistent with cellular findings, silencing ALYREF significantly inhibited tumor growth <em>in vivo</em> using xenograft models.</div></div><div><h3>Conclusions</h3><div>This study demonstrates that ALYREF drives colon cancer malignancy by stabilizing PCSK9 messenger RNA via m5C methylation, thereby enhancing PCSK9 expression. These findings establish the ALYREF/PCSK9 axis as a critical mechanism in colon cancer progression, highlighting its potential as a novel therapeutic target for intervention.</div><div><strong>How to cite:</strong> Cao L, Chen Y, Yu J, et al. ALYREF promotes malignant behaviors and inhibits ferroptosis in colon cancer cells by stabilizing PCSK9 mRNA. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 53-63"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qualitative phytochemical analysis, thin-layer chromatographic profiling, and antimicrobial potential of banana cultivars","authors":"Ajmal Khan ,&nbsp;Rony Swennen ,&nbsp;Sujogya Kumar Panda ,&nbsp;Liliane Schoofs ,&nbsp;Walter Luyten","doi":"10.1016/j.ejbt.2025.07.005","DOIUrl":"10.1016/j.ejbt.2025.07.005","url":null,"abstract":"<div><h3>Background</h3><div>Banana plants possess numerous medicinal properties due to the presence of various phytochemicals. This study aimed to assess the phytochemical profile of the crude extracts of leaf, pseudostem, and corm parts of selected banana cultivars via standard techniques and thin-layer chromatography (TLC) and to evaluate their antimicrobial activities against several food-borne and clinically important human pathogens, including two Gram-positive bacteria, six Gram-negative bacteria, and four yeasts.</div></div><div><h3>Results</h3><div>The results demonstrated that the Cachaco (41 %), Tereza (38 %), Fougamou (30 %), Pelipita (28 %), Giant Cavendish (26 %), and Kluai Teparot (26 %) cultivars presented significant antimicrobial activity against pathogens compared with Dole (24 %), Namwah Khom (20 %), and Mbwazirume (16 %) cultivars. Moreover, the leaves (40 %) of cultivars extracted in water (61 %) and acetone (55 %) yielded the most active antimicrobial extracts compared with the pseudostem (33 %) and corm (26 %) extracts prepared in ethanol (38 %) or hexane (28 %). Overall, the antimicrobial activities with the lowest 50 % inhibitory concentration (IC₅₀) values, especially those with values less than 200 µg/mL for bacteria and 100 µg/mL for yeasts, were reported in the leaves of Cachaco and Giant Cavendish, followed by different parts of Tereza, Pelipita, and other banana cultivars. Phytochemical analysis and TLC profiling confirmed the presence of various groups of phytochemicals in the extracts of the selected banana cultivars.</div></div><div><h3>Conclusions</h3><div>This study revealed that the Cachaco, Giant Cavendish, Pelipita, and Tereza cultivars possess significant antimicrobial activity, warranting further bioassay-guided antimicrobial studies for the isolation and identification of bioactive compounds, which could be useful as novel drug candidates with the highest potency.</div><div><strong>How to cite:</strong> Khan A, Swennen R, Panda SK, et al. Qualitative phytochemical analysis, thin-layer chromatographic profiling, and antimicrobial potential of banana cultivars. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 64-75"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome analysis and biosynthetic gene cluster profiling of Stenotrophomonas sp. ASucR1 isolated from Sof Umer Cave, Ethiopia","authors":"Abu Feyisa Meka ,&nbsp;Gessesse Kebede Bekele ,&nbsp;Selfu Girma Gebre ,&nbsp;Musin Kelel Abas ,&nbsp;Mesfin Tafesse Gemeda","doi":"10.1016/j.ejbt.2025.07.004","DOIUrl":"10.1016/j.ejbt.2025.07.004","url":null,"abstract":"<div><h3>Background</h3><div>Sof Umer Cave is a unique habitat that hosts industrially significant microbes. In this study, <em>Stenotrophomonas</em> sp. ASucR1 was isolated from the cave rock and screened for antimicrobial activity. High-molecular-weight genomic DNA was extracted and subjected to whole-genome sequencing using the Illumina NovaSeq platform. Comprehensive genomic and biosynthetic gene cluster (BGC) profiling was conducted.</div></div><div><h3>Results</h3><div><em>In vitro</em> tests revealed that <em>Stenotrophomonas</em> sp. ASucR1 exhibited a broad spectrum of antagonistic activity. Functional genome annotation identified diverse biosynthetic gene clusters (BGCs) and metabolic pathways, including genes involved in the synthesis of secondary metabolites. A total of 19 BGCs were identified, several of which showed no matches in the minimum information about a biosynthetic gene cluster (MiBIG) database, indicating the presence of previously uncharacterized bioactive compounds. Single-nucleotide polymorphism (SNP) analysis showed that 91.5% of variants were identified within coding regions, with 85.84% being synonymous. Classification of SNPs and insertion-deletion mutations through clusters of orthologous groups (COG) analysis highlighted their association with key biological functions.</div></div><div><h3>Conclusions</h3><div>This study highlights the metabolic versatility and biosynthetic potential of <em>Stenotrophomonas</em> sp. ASucR1, a promising candidate for antimicrobial development and biotechnological applications. The identification of various biosynthetic gene clusters paves the way for exploring bioactive compounds with pharmaceutical significance.</div><div><strong>How to cite:</strong> Meka AF, Bekele GK, Gebre SG, et al. Whole genome analysis and biosynthetic gene cluster profiling of <em>Stenotrophomonas</em> sp. ASucR1 isolated from Sof Umer Cave, Ethiopia. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 46-52"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tianma granules: Bridging traditional medicine and modern science to combat colorectal cancer via ferroptosis","authors":"Ning Ding ,&nbsp;Xiaojuan Tang ,&nbsp;Yijing Zhang ,&nbsp;Hongbiao Luo ,&nbsp;Yanbo Tang ,&nbsp;Chaoqun Zeng ,&nbsp;Yongheng He ,&nbsp;Liang Zhao","doi":"10.1016/j.ejbt.2025.06.004","DOIUrl":"10.1016/j.ejbt.2025.06.004","url":null,"abstract":"<div><h3>Background</h3><div>This study aims to investigate the ferroptosis-inducing effects of Tianma Granules (TMGs) in colorectal cancer and elucidate its molecular mechanisms. Ferroptosis, an iron-dependent form of regulated cell death, represents a novel therapeutic target for cancer. We combined network pharmacology with experimental validation to explore TMG’s anti-cancer potential through ferroptosis modulation.</div></div><div><h3>Results</h3><div>Network pharmacology identified 382 ferroptosis-related genes overlapping with 12,944 CRC-associated targets (<em>p</em> &lt; 0.05), with SLC7A11, GPX4, SAT1, PTGS2, and GLS2 prioritized as core targets. <em>In vitro</em>, TMG dose-dependently suppressed CRC cell proliferation (<em>p</em> &lt; 0.05), elevated reactive oxygen species (<em>p</em> &lt; 0.05) and ferrous ion levels (<em>p</em> &lt; 0.01), effects reversed by ferroptosis inhibitor, Ferrostatin-1. c-Casp3 levels were unchanged (<em>p</em> &gt; 0.05), excluding apoptosis. Transmission electron microscopy revealed mitochondrial cristae fragmentation and vacuolation, hallmark features of ferroptosis. Molecular analyses demonstrated TMG-mediated downregulation of SLC7A11 and GPX4, alongside upregulation of SAT1, PTGS2, and GLS2 (<em>p</em> &lt; 0.05). In xenograft models, high-dose TMG (23.2 g/kg) reduced tumor volume, attenuated cachexia, and elevated intratumoral ROS and Fe²⁺ levels (<em>p</em> &lt; 0.01), corroborating ferroptosis induction <em>in vivo</em>.</div></div><div><h3>Conclusions</h3><div>TMG suppresses CRC progression by inducing ferroptosis via dual inhibition of SLC7A11/GPX4 and activation of SAT1/PTGS2/GLS2. This study bridges traditional medicine and ferroptosis biology, positioning TMG as a novel therapeutic candidate for CRC.</div><div><strong>How to cite:</strong> Ding N, Tang X, Zhang Y, et al. Tianma granules: Bridging traditional medicine and modern science to combat colorectal cancer via ferroptosis. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.06.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 14-25"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chufeng Yisun Decoction treats dry eye syndrome by inhibiting the PI3K/Akt pathway","authors":"Yue Du ,&nbsp;Xue Jiang ,&nbsp;Yanyan Zhang ,&nbsp;Quanyong Yi","doi":"10.1016/j.ejbt.2025.05.006","DOIUrl":"10.1016/j.ejbt.2025.05.006","url":null,"abstract":"<div><h3>Background</h3><div>Dry eye disease seriously affects people’s work and life. Chufeng Yisun Decoction is a traditional Chinese medicine decoction used in treating dry eye disease. This study aims to explore the core active ingredients, targets, and mechanisms of CFYSD in dry eye disease, providing new insights for the dry eye disease treatment.</div></div><div><h3>Results</h3><div>A total of 196 target genes were screened from Chufeng Yisun Decoction, and 170 genes were related to dry eye disease. Gene Ontology and KEGG enrichment analyses showed that Chufeng Yisun Decoction influenced dry eye disease through “Lipid and atherosclerosis”, “Fluid shear stress and atherosclerosis”, and “PI3K-Akt”. The core targets of Chufeng Yisun Decoction in treating dry eye disease were Akt1 and IL-1β. The core active ingredients were kaempferol, wogonin, and quercetin. Molecular docking results showed that the binding energies of kaempferol and Akt1, wogonin and Akt1, quercetin and Akt1, and quercetin and IL-1β were −6.1, −6.1, −6.1, and −7.9 kcal/mol, respectively. Chufeng Yisun Decoction significantly alleviated cell damage and reduced PI3K/Akt pathway-related protein expression. PI3K activation partially reversed the therapeutic effect of Chufeng Yisun Decoction on dry eye disease.</div></div><div><h3>Conclusions</h3><div>Chufeng Yisun Decoction treats dry eye disease by inactivating the PI3K/Akt pathway through multi-ingredients and multi-targets.</div><div><strong>How to cite:</strong> Du Y, Jiang X, Zhang Y, et al. Chufeng Yisun Decoction treat dry eye syndrome by inhibiting the PI3K/Akt pathway. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 26-34"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway","authors":"Yanlin Tan ,&nbsp;Jun Gao","doi":"10.1016/j.ejbt.2025.07.006","DOIUrl":"10.1016/j.ejbt.2025.07.006","url":null,"abstract":"<div><h3>Background</h3><div>N6-methyladenosine (m⁶A) methylation plays a key role in osteosarcoma (OS) progression. This study aimed to elucidate the function and mechanism of methyltransferase 16 (METTL16), an m⁶A methyltransferase, in OS progression.</div></div><div><h3>Results</h3><div>Bioinformatics analysis with quantitative reverse-transcription polymerase chain reaction (qRT-PCR) revealed high METTL16 expression in OS. After performing cell functional experiments, METTL16 silencing was shown to decrease the proliferation, migration, and invasion of OS cells. Using qRT-PCR, methylated RNA immunoprecipitation quantitative polymerase chain reaction (MeRIP-qPCR), Western blotting, luciferase, RNA-binding protein immunoprecipitation (RIP), and RNA stability assays, METTL16 induced the m⁶A methylation of ubiquitin protein ligase E3A (UBE3A) to promote UBE3A expression and mRNA stability in OS cells in a fragile X messenger ribonucleoprotein 1 (FMR1)-dependent manner. Moreover, <em>in vitro</em> and <em>in vivo</em> results showed that UBE3A activated the Notch signaling pathway, thereby promoting OS cell malignancy. METTL16 knockdown partly reversed the oncogenic role of UBE3A in OS cells.</div></div><div><h3>Conclusions</h3><div>METTL16 acts as a tumor promotor in OS progression by modulating UBE3A expression via m⁶A methylation to activate the Notch signaling pathway. The findings highlight the therapeutic potential of disrupting the METTL16–UBE3A–Notch pathway axis in OS.</div><div><strong>How to cite:</strong> Tan Y, Gao J. METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 86-95"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}