Electronic Journal of Biotechnology最新文献

{"title":"Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge","authors":"Chutema Thongsongkaew ,&nbsp;Benjamas Cheirsilp ,&nbsp;Asma Billateh ,&nbsp;Wageeporn Maneechote ,&nbsp;Sirasit Srinuanpan","doi":"10.1016/j.ejbt.2025.05.005","DOIUrl":"10.1016/j.ejbt.2025.05.005","url":null,"abstract":"<div><h3>Background</h3><div>Aquaculture has become the fastest-growing sector in recent decades, and this has led to intensified cultural practices to achieve high yields. However, such practices have raised concerns regarding their environmental impact, as aquaculture sludge and wastewater cause significant organic pollution. This study then aimed to isolate and screen proteolytic, amylolytic, and lipolytic bacteria and evaluate their consortia for enzyme production and bioremediation of shrimp pond sludge.</div></div><div><h3>Results</h3><div>The strategy using multiple substrates in the isolation media successfully obtained bacterial strains with multiple hydrolytic activities. After primary and secondary quantitative screening, 18 isolates that exhibited high dual and triple hydrolytic activities were selected. After tertiary quantitative screening using synthetic shrimp pond sludge and co-culture tests, <em>Exiguobacterium indicum</em> SSP-PA-08, <em>Bacillus coagulans</em>, and <em>Bacillus subtilis</em> were selected due to their synergy for the production of triple hydrolytic enzymes. Their consortia inoculated in shrimp pond sludge containing 0.8% total suspended solids (TSSs) showed an increase in proteolytic activity by 2.5 folds and amylolytic activity by 20 folds, which led to a greater reduction in TSS. The highest enzyme production was obtained using shrimp pond sludge containing 1.6% TSS.</div></div><div><h3>Conclusions</h3><div>This study has developed the methods to isolate bacteria with multienzyme-producing ability. Co-culturing these bacteria in synthetic shrimp sludge significantly enhanced hydrolytic activity and led to a greater reduction in TSS. These strategies may contribute greatly to the hydrolytic enzyme production and environmentally friendly bioremediation of aquaculture sludge.</div><div><strong>How to cite:</strong> Thongsongkaew C, Cheirsilp B, Billateh A, et al. Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 35-47"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved resistance to basal rot disease and promotion of onion plant growth by Aspergillus terreus-mediated silver nanoparticles","authors":"Mohamed G.A. Hegazy ,&nbsp;Mahmoud Gad ,&nbsp;Sobhi F. Lamlom ,&nbsp;Islam I. Teiba ,&nbsp;Osama A.M. Al-Bedak ,&nbsp;Muhammad Moaaz Ali ,&nbsp;Eman Alhomaidi ,&nbsp;Mona Saleh Al Tami ,&nbsp;Ahmed F. Yousef ,&nbsp;Waleed M. Ali","doi":"10.1016/j.ejbt.2025.05.003","DOIUrl":"10.1016/j.ejbt.2025.05.003","url":null,"abstract":"<div><h3>Background</h3><div>Onions, a vital agricultural crop rich in carbohydrates and essential minerals, face severe threats from <em>Fusarium oxysporum</em>, the causative agent of basal rot disease. This study assessed the effectiveness of extracellular, green-synthesized silver nanoparticles (AgNPs), produced by <em>Aspergillus terreus</em> AUMC 15760, in managing basal rot disease caused by <em>F. oxysporum</em> AUMC 15798.</div></div><div><h3>Results</h3><div>AgNPs ranging from 12.1 to 28.7 nm were incorporated into PDA media at concentrations of 50, 100, 150, and 200 ppm, achieving fungal growth inhibition rates of 24.88%, 40.77%, 54.44%, and 69.33%, respectively. A greenhouse experiment was carried out using onion seedlings, with a randomized complete block design (RCBD) to compare eight different treatments: 100 ppm AgNPs (spray), 50 ppm AgNPs (spray), 100 ppm AgNPs (soil application), 50 ppm AgNPs (soil application), Dovex spray (50%), Dovex soil application (50%), a negative control, and a positive control. Greenhouse results showed a significant reduction in disease severity, with Dovex lowering it to 20%. AgNPs at 50 ppm reduced severity to 57.77% (soil) and 35.55% (spray), while 100 ppm further decreased it to 31.1% (soil) and 22.2% (spray). The application of 100 ppm AgNPs improved plant growth parameters. It also enhanced chlorophyll <em>a</em>, chlorophyll <em>b</em>, and carotenoid levels. The greatest reductions in phenolic (0.34 mg/g) and anthocyanin contents (0.48 mg/g), as well as peroxidase (0.44 µmol/min) and catalase activities (0.19 µmol/min), were recorded in plants treated with 100 ppm AgNPs (spray).</div></div><div><h3>Conclusions</h3><div>AgNPs effectively control basal rot disease, boost plant growth, and regulate antioxidant activity.</div><div><strong>How to cite:</strong> Hegazy MG, Gad M, Lamlom SF, et al. Improved resistance to basal rot disease and promotion of onion plant growth by <em>Aspergillus terreus</em>-mediated silver nanoparticles. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 24-34"},"PeriodicalIF":2.5,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biological agent rituximab ameliorates DNA damage and repair efficiency in the somatic cells of arthritic mice","authors":"Mohammed A. Al-Hamamah ,&nbsp;Mohamed A. Mahmoud ,&nbsp;Moureq R. Alotaibi ,&nbsp;Ahmed Nadeem ,&nbsp;Mushtaq A. Ansari ,&nbsp;Sheikh F. Ahmad ,&nbsp;Saleh A. Bakheet ,&nbsp;Gamaleldin I. Harisa ,&nbsp;Sabry M. Attia","doi":"10.1016/j.ejbt.2025.03.004","DOIUrl":"10.1016/j.ejbt.2025.03.004","url":null,"abstract":"<div><h3>Background</h3><div>Rheumatoid arthritis (RA) is an autoimmune disorder that deteriorates joints and can affect various physiological systems if not properly managed. The persistent joint inflammation and oxidative damage caused by RA can lead to genomic instability, a primary feature of most cancer cells. In RA, the prevalence of malignancy is comparatively higher than in the general population. However, it is unclear if the disease itself or its treatments induce susceptibility to neoplastic disorders. Our goal was to study genomic instability in the arthritic mouse model, namely DNA damage and repair and determine if the long-term use of the biological agent rituximab can impact these changes.</div></div><div><h3>Results</h3><div>Our results show that rituximab did not disrupt genomic stability at the tested regimen. Arthritic mice had more spontaneous DNA damage and a slower repair rate than control mice. Redox imbalance, oxidative DNA damage, and disturbance in the DNA repair pathways are also increased in arthritic mice. Meanwhile, rituximab treatment reduced oxidative DNA damage and accelerated DNA repair rate, improving redox balance and restoring the disturbances in the DNA repair gene expression at both mRNA as determined by RT² Profiler PCR array and protein levels as determined by Western blotting analysis in arthritic animals. Additionally, the ameliorative effect of rituximab has also been shown by its ability to reduce the severity of joint inflammation and histopathological alterations induced by arthritis.</div></div><div><h3>Conclusions</h3><div>The findings indicate that rituximab is a non-genotoxic, safe, effective drug for the treatment of RA and its complications.</div><div><strong>How to cite:</strong> Al-Hamamah MA, Mahmoud MA, Alotaibi MR, et al. The biological agent rituximab ameliorates DNA damage and repair efficiency in the somatic cells of arthritic mice. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 67-78"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal community dynamics and pathogenic risk in composting, vermicomposting and leachate systems amended with rabbit manure","authors":"Rocío del Pilar Serrano-Ramírez ,&nbsp;Adalberto Zenteno-Rojas ,&nbsp;Valentín Pérez-Hernández ,&nbsp;Odín Reyes-Vallejo ,&nbsp;Sugey Vásquez-Hernández ,&nbsp;Héctor Hiram Torres-Ventura ,&nbsp;Mayram Margarita González-Reyes ,&nbsp;Wilber Montejo-López ,&nbsp;Abumalé Cruz-Salomón ,&nbsp;Joaquín Adolfo Montes-Molina","doi":"10.1016/j.ejbt.2025.05.002","DOIUrl":"10.1016/j.ejbt.2025.05.002","url":null,"abstract":"<div><h3>Background</h3><div>Understanding the dynamics of fungal communities in composting and vermicomposting systems is essential for optimizing waste management practices and minimizing pathogen risks. For this reason, this study assessed the fungal community structure and potential pathogenic risks in composting, vermicomposting and leachate systems amended with rabbit manure, using Illumina’s MiSeq platform for internal transcribed spacer (ITS) sequencing and FUNGuild analysis.</div></div><div><h3>Results</h3><div>Phylotypes from Basidiomycota were predominant in all treatments, while the pathogenic genus <em>Microascus</em>, initially abundant (37%), showed a significant reduction to 5% and 7% following composting and vermicomposting, respectively, and to 1% and 13% in their leachates. Given <em>Microascus</em>’s association with human skin diseases, proper handling of organic waste is critical before its agricultural use. In contrast, the FUNGuild analysis revealed a high abundance of saprotrophic fungi such as <em>Aspergillus</em>, <em>Preussia, Botryotrichum</em>, and <em>Acremonium</em> known for producing enzymes that promote nutrient cycling and soil fertility.</div></div><div><h3>Conclusions</h3><div>These findings highlight the potential for vermicomposting to reduce pathogen risks while enhancing fungal-driven nutrient recycling, offering practical insights for sustainable agriculture and organic waste management.</div><div><strong>How to cite:</strong> Serrano-Ramírez RdP, Zenteno-Rojas A, Pérez-Hernández V, et al. Fungal community dynamics and pathogenic risk in composting, vermicomposting and leachate systems amended with rabbit manure. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 1-11"},"PeriodicalIF":2.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of mitochondrial genomes to species identification and evolution","authors":"Bingpeng Xing ,&nbsp;Liangyu Lin ,&nbsp;Qiong Wu","doi":"10.1016/j.ejbt.2025.04.001","DOIUrl":"10.1016/j.ejbt.2025.04.001","url":null,"abstract":"<div><div>Mitochondrial genomes (mtDNA) have become invaluable in species classification and evolutionary studies due to their unique characteristics, including maternal inheritance, and high mutation rates. This review examines the application of mtDNA in tracing evolutionary history, elucidating phylogenetic relationships, and understanding mechanisms of species divergence. The evolution of mitochondrial DNA research from its initial focus on energy metabolism to its current role in biodiversity assessments highlights its significance in modern biology. Mitochondrial DNA barcoding, particularly utilizing the cytochrome <em>c</em> oxidase I (COI) gene, has revolutionized species identification, enabling rapid and accurate classification across diverse taxa. The article further explores the implications of mtDNA in understanding adaptive evolution, as genetic variations within mitochondrial genomes can reveal insights into how species respond to environmental pressures. However, challenges such as gene mixing, hybridization, and incomplete lineage sorting can complicate interpretations of mtDNA data. Thus, integrating mitochondrial with nuclear genome data is advocated to provide a comprehensive view of species relationships and evolutionary patterns. Future research directions emphasize the need for multi-genome studies, investigations into ecological adaptations, and exploration of understudied taxa and ecosystems, which are crucial for enhancing our understanding of biodiversity and informing conservation strategies.</div><div><strong>How to cite:</strong> Xing B, Lin L, Wu Q. Application of mitochondrial genomes to species identification and evolution. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.04.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 39-48"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of heterotrophic nitrifying/aerobic denitrifying strain Pseudomonas stutzeri YXH-102 to hypersaline stress","authors":"Youming Wang ,&nbsp;Zhichun Wang ,&nbsp;Yangfan Hu ,&nbsp;Dexun Zou ,&nbsp;Korakot Sombatmankhong ,&nbsp;Wen Wang ,&nbsp;Bo Yu","doi":"10.1016/j.ejbt.2025.05.001","DOIUrl":"10.1016/j.ejbt.2025.05.001","url":null,"abstract":"<div><h3>Background</h3><div>Hypersaline wastewater poses significant environmental challenges, necessitating efficient bioremediation strategies. This study investigates the hypersaline tolerance mechanisms of <em>Pseudomonas stutzeri</em> YXH-102, a heterotrophic nitrifying/aerobic denitrifying bacterium isolated from Yuncheng Salt Lake sediments, under high-salinity stress.</div></div><div><h3>Results</h3><div>Comparative transcriptomic analysis revealed 268 differentially expressed genes (DEGs) in response to a 10 % NaCl shock, with 86 upregulated and 182 downregulated. Key findings highlight the critical roles of oxidative stress mitigation, energy metabolism adaptation, and ion homeostasis. Salt stress triggered reactive oxygen species (ROS) accumulation, countered by upregulated cytochrome <em>c</em> oxidase (reducing ROS generation) and glutathione S-transferase (enhancing ROS scavenging). Concurrently, energy metabolism pathways, including fatty acid β-oxidation and acetyl-CoA production, were activated to sustain cellular energy demand. Notably, the electron transport chain (ETC) generated a robust proton motive force (PMF), which directly fueled potassium uptake via H⁺/K⁺ symporters to counteract osmotic imbalance. TonB-dependent transporters for nutrient uptake were also significantly upregulated, suggesting enhanced nutrient acquisition under salinity.</div></div><div><h3>Conclusions</h3><div>These findings elucidate how <em>P. stutzeri</em> YXH-102 combats salt stress through integrated ROS detoxification, energy optimization, and PMF-driven ion transport, providing molecular insights for its application in hypersaline wastewater bioremediation.</div><div><strong>How to cite:</strong> Wang Y, Wang Z, Hu Y, et al. Response of heterotrophic nitrifying/aerobic denitrifying strain <em>Pseudomonas stutzeri</em> YXH-102 to hypersaline stress. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 49-56"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of flaxseed’s supplement on AMPKα2′s expression in non-diabetic and diabetic patients having follicular thyroid carcinoma on mitochondrial’s ATP synthase for TPC-1 cell line","authors":"Amena Alsakran ,&nbsp;Afaf Aldahish ,&nbsp;Amani Alotaibi ,&nbsp;Dalal Alshaya ,&nbsp;Elham Alzahrani ,&nbsp;Mohammad Alshugeer ,&nbsp;Manal Alduwish ,&nbsp;Dalia Domiaty ,&nbsp;Fatimah Albani ,&nbsp;Wedad Al-Qahtani","doi":"10.1016/j.ejbt.2025.03.006","DOIUrl":"10.1016/j.ejbt.2025.03.006","url":null,"abstract":"<div><h3>Background</h3><div>This study examined the effects of flaxseed oil supplementation on the expression of adenosine monophosphate-activated protein kinase alpha2 (AMPKα2) in male patients with follicular thyroid cancer. The objective was to assess whether flaxseed oil could enhance AMPKα2 activity, improve histological features, and reduce tumor cell viability, offering a potential therapeutic benefit for diabetic and non-diabetic patients.</div></div><div><h3>Results</h3><div>A total of 303 patients, including 149 non-diabetic and 154 diabetic participants, received 1250 mg of flaxseed oil daily for one month. Flaxseed oil supplementation significantly improved tumor histology, with treated tissues showing organized follicular patterns and reduced fibrovascular cells compared to untreated tissues. Cell viability assays demonstrated a dose-dependent reduction in papillary thyroid carcinoma cell viability, with the strongest effects observed at flaxseed extract concentrations of 80, 50, and 30 µg/mL. Molecular analyses revealed increased AMPKα2 expression in both treated thyroid cancer tissues and cell line models, indicating enhanced protein activation. These findings suggest that flaxseed oil supplementation may inhibit tumor proliferation and improve histological organization in follicular thyroid cancer.</div></div><div><h3>Conclusions</h3><div>Flaxseed oil supplementation activates AMPKα2, reduces tumor cell viability, and improves histopathological features in follicular thyroid cancer tissues. These results highlight the potential of flaxseed oil as an adjunctive therapy to enhance cancer management strategies, particularly in diabetic and non-diabetic patients with follicular thyroid cancer.</div><div><strong>How to cite:</strong> Alsakran A, Aldahish A, Alotaibi A, et al. Effect of flaxseed’s supplement on AMPKα2’s expression in non-diabetic and diabetic patients having follicular thyroid carcinoma on mitochondrial’s ATP synthase for TPC-1 cell line. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 57-66"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the process conditions for the biosorption of chromium (VI) by Bacillus subtilis in artificial wastewater","authors":"Khawla E. Alsamhary","doi":"10.1016/j.ejbt.2025.03.005","DOIUrl":"10.1016/j.ejbt.2025.03.005","url":null,"abstract":"<div><h3>Background</h3><div>The contamination by heavy metals, particularly hexavalent chromium Cr (VI), is a pressing environmental concern. Cr(VI) is highly toxic, non-biodegradable and carcinogenic. Traditional remediation methods are often costly, energy-intensive, or generate secondary waste. This study explores the optimal conditions for the bacterium <em>Bacillus subtilis</em> in reducing Cr(VI) from synthetic wastewater.</div></div><div><h3>Results</h3><div>The research employed batch experiments to simulate wastewater treatment. The removal of Cr(VI) was measured spectrophotometrically. The active functional groups were studied using Fourier Transform Infrared Spectroscopy that showed an incremental shift for alkyl halides (500.75 cm⁻¹) and OH-groups (3347 cm⁻¹) were observed. Scanning Electron Microscopy images demonstrated that the surface morphology of the biosorbent was more homogenous before than after adsorption. The biosorbent’s structure was confirmed by a prominent peak in X-ray Diffraction at 290.04°. The highest adsorption was observed at the adsorbent dose of 0.5 g/L, the contact time 60 min, pH 6 and temperature of 40°C. The thermodynamic parameters validated the process’s feasibility and spontaneity. Several models for biosorption kinetics and isotherm were tested. The pseudo-second-order was more suitable than the pseudo-first-order model. Langmuir isotherm model had the best fit compared to Freundlich, Dubinin–Radushkevich, and Temkin models.</div></div><div><h3>Conclusions</h3><div><em>B. subtilis</em> appeared to be resistant to chromium and reduce Cr(VI) efficiently. This study shows the potential of <em>B. subtilis</em> as a viable bioremediation agent for Cr(VI) contamination in wastewater and should be studied further using real wastewater with different pollutants.</div><div><strong>How to cite:</strong> Alsamhary KE. Optimizing the process conditions for the biosorption of chromium (VI) by <em>Bacillus subtilis</em> in artificial wastewater. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 22-38"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of isothermal amplification technology: A bibliometric study (2013–2022)","authors":"Hanting Zhu ,&nbsp;Zhitong Miao ,&nbsp;Junfei Yuan ,&nbsp;Wanying Xie ,&nbsp;Qiaoqiao Zhang ,&nbsp;Kun Yang","doi":"10.1016/j.ejbt.2025.03.003","DOIUrl":"10.1016/j.ejbt.2025.03.003","url":null,"abstract":"<div><h3>Background</h3><div>Isothermal amplification is a nucleic acid amplification technology (NAAT) that has contributed significantly to molecular diagnostics. The combination of NAAT with a suitable detection platform has improved sensitivity and specificity and enabled rapid disease diagnosis. A total of 5388 articles relating to isothermal amplification technology published from 2013 to 2022 were identified in the Web of Science Core Collection (WoSCC) database and subsequently analyzed with CiteSpace 5.7.R1 software.</div></div><div><h3>Results</h3><div>The number of published articles on isothermal amplification technology steadily increased between 2013 and 2022. The disciplines included Chemistry, Science Technology Other Topics, Biotechnology Applied Microbiology, Microbiology, and Biochemistry Molecular Biology. The countries with the highest number of published articles were China, the United States, and Japan while the institutions with the highest number of published articles were the Chinese Academy of Sciences, the Chinese Academy of Agricultural Sciences, and Mahidol University. The high-frequency keywords included rapid detection, sensitivity, pathogen, <em>Escherichia coli</em>, probe, primer, and expression. Over recent years, most research studies concerning isothermal amplification focused on its advantages, improvements, and applications. RCA, LAMP, RPA, and Cas technologies appeared sequentially from 2013 onward. The keyword “LAMP” exhibited the highest frequency (1222 times).</div></div><div><h3>Conclusions</h3><div>Our study described the research trends and the status of isothermal amplification technology over the past decade, providing guidance for future research in this field.</div><div><strong>How to cite:</strong> Zhu H, Miao Z, Yuan J, et al. The evolution of isothermal amplification technology: A bibliometric study (2013–2022). Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 12-21"},"PeriodicalIF":2.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin mitigates potassium bromate-induced liver grievance in rat through attenuating NF-kB and PI3K/Akt pathway","authors":"Bo Ma ,&nbsp;Sheng Zheng ,&nbsp;Ning Xie ,&nbsp;Juan Yang ,&nbsp;Xueli Zeng ,&nbsp;Pei Liu ,&nbsp;Shunling Zhang ,&nbsp;Ji Li","doi":"10.1016/j.ejbt.2025.03.002","DOIUrl":"10.1016/j.ejbt.2025.03.002","url":null,"abstract":"<div><h3>Background</h3><div>Metformin (MET) is a dietary polyphenolic compound that exhibits anti-inflammatory and antioxidant properties. This study evaluated the protective effects of MET in both <em>in vitro</em> and <em>in vivo</em> models against potassium bromate (KBrO₃)-induced hepatotoxicity. Hepatic cells were exposed to KBrO₃ with or without metformin (20, 40, and 60 µM), and cell viability and Reactive Oxygen Species levels were assessed. <em>In vivo,</em> rats were divided into five groups: control, KBrO₃, and KBrO₃ with metformin (25, 50, and 100 mg/kg). Liver and blood samples were analyzed for histological changes, oxidative stress markers, lipid peroxidation, liver enzymes, and PI3K/Akt signaling.</div></div><div><h3>Results</h3><div>KBrO₃ exposure significantly decreased cell viability and increased ROS levels. Co-treatment with MET dose-dependently restored cell viability, with 60 µM MET achieving approximately 80% viability. Metformin also reduced ROS levels, with mean fluorescence intensity approaching control values at higher concentrations. In the <em>in vivo</em> study, KBrO₃ exposure elevated lipid peroxidation markers, depleted antioxidant enzyme activities, and triggered oxidative stress and inflammation. Metformin significantly alleviated histological liver damage, suppressed proinflammatory cytokines, enhanced antioxidant enzyme activities, and modulated the PI3K/Akt signaling pathway to promote cell survival and reduce oxidative injury.</div></div><div><h3>Conclusions</h3><div>Metformin effectively protects hepatic cells against KBrO₃-induced cytotoxicity by improving cell viability and reducing Reactive Oxygen Species levels. Metformin successfully mitigates KBrO₃-induced hepatic injury by reducing oxidative stress, modulating inflammatory pathways (NF-kB), and regulating the PI3K/Akt signaling cascade, offering molecular evidence of its hepatoprotective effects.</div><div><strong>How to cite:</strong> Ma B, Zheng S, Xie N, et al. Metformin mitigates potassium bromate induced liver grievance in rat through attenuating NF-kB and PI3K/Akt pathway. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 1-11"},"PeriodicalIF":2.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}