Electronic Journal of Biotechnology最新文献

{"title":"Chufeng Yisun Decoction treats dry eye syndrome by inhibiting the PI3K/Akt pathway","authors":"Yue Du ,&nbsp;Xue Jiang ,&nbsp;Yanyan Zhang ,&nbsp;Quanyong Yi","doi":"10.1016/j.ejbt.2025.05.006","DOIUrl":"10.1016/j.ejbt.2025.05.006","url":null,"abstract":"<div><h3>Background</h3><div>Dry eye disease seriously affects people’s work and life. Chufeng Yisun Decoction is a traditional Chinese medicine decoction used in treating dry eye disease. This study aims to explore the core active ingredients, targets, and mechanisms of CFYSD in dry eye disease, providing new insights for the dry eye disease treatment.</div></div><div><h3>Results</h3><div>A total of 196 target genes were screened from Chufeng Yisun Decoction, and 170 genes were related to dry eye disease. Gene Ontology and KEGG enrichment analyses showed that Chufeng Yisun Decoction influenced dry eye disease through “Lipid and atherosclerosis”, “Fluid shear stress and atherosclerosis”, and “PI3K-Akt”. The core targets of Chufeng Yisun Decoction in treating dry eye disease were Akt1 and IL-1β. The core active ingredients were kaempferol, wogonin, and quercetin. Molecular docking results showed that the binding energies of kaempferol and Akt1, wogonin and Akt1, quercetin and Akt1, and quercetin and IL-1β were −6.1, −6.1, −6.1, and −7.9 kcal/mol, respectively. Chufeng Yisun Decoction significantly alleviated cell damage and reduced PI3K/Akt pathway-related protein expression. PI3K activation partially reversed the therapeutic effect of Chufeng Yisun Decoction on dry eye disease.</div></div><div><h3>Conclusions</h3><div>Chufeng Yisun Decoction treats dry eye disease by inactivating the PI3K/Akt pathway through multi-ingredients and multi-targets.</div><div><strong>How to cite:</strong> Du Y, Jiang X, Zhang Y, et al. Chufeng Yisun Decoction treat dry eye syndrome by inhibiting the PI3K/Akt pathway. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 26-34"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway","authors":"Yanlin Tan ,&nbsp;Jun Gao","doi":"10.1016/j.ejbt.2025.07.006","DOIUrl":"10.1016/j.ejbt.2025.07.006","url":null,"abstract":"<div><h3>Background</h3><div>N6-methyladenosine (m⁶A) methylation plays a key role in osteosarcoma (OS) progression. This study aimed to elucidate the function and mechanism of methyltransferase 16 (METTL16), an m⁶A methyltransferase, in OS progression.</div></div><div><h3>Results</h3><div>Bioinformatics analysis with quantitative reverse-transcription polymerase chain reaction (qRT-PCR) revealed high METTL16 expression in OS. After performing cell functional experiments, METTL16 silencing was shown to decrease the proliferation, migration, and invasion of OS cells. Using qRT-PCR, methylated RNA immunoprecipitation quantitative polymerase chain reaction (MeRIP-qPCR), Western blotting, luciferase, RNA-binding protein immunoprecipitation (RIP), and RNA stability assays, METTL16 induced the m⁶A methylation of ubiquitin protein ligase E3A (UBE3A) to promote UBE3A expression and mRNA stability in OS cells in a fragile X messenger ribonucleoprotein 1 (FMR1)-dependent manner. Moreover, <em>in vitro</em> and <em>in vivo</em> results showed that UBE3A activated the Notch signaling pathway, thereby promoting OS cell malignancy. METTL16 knockdown partly reversed the oncogenic role of UBE3A in OS cells.</div></div><div><h3>Conclusions</h3><div>METTL16 acts as a tumor promotor in OS progression by modulating UBE3A expression via m⁶A methylation to activate the Notch signaling pathway. The findings highlight the therapeutic potential of disrupting the METTL16–UBE3A–Notch pathway axis in OS.</div><div><strong>How to cite:</strong> Tan Y, Gao J. METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 86-95"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kaixin San in treating vascular dementia via regulating the Bcl-2/Beclin-1/LC3A/B signaling pathway via animal experiments and network pharmacology analysis","authors":"Meng-qi Li ,&nbsp;Yu-cheng Lu ,&nbsp;Yan-chun Li ,&nbsp;Yu-fu Zeng ,&nbsp;Ying-ying Cao ,&nbsp;Ling Zhang ,&nbsp;Ben Chen ,&nbsp;Ling Chen ,&nbsp;Wei-an Qiu ,&nbsp;Zi-heng Huang","doi":"10.1016/j.ejbt.2025.08.001","DOIUrl":"10.1016/j.ejbt.2025.08.001","url":null,"abstract":"<div><h3>Background</h3><div>Kaixin San (KXS), a traditional Chinese herbal formula, is used to treat vascular dementia (VaD), but its active ingredients and mechanisms remain unclear. This study combined animal experiments with network pharmacology to explore how KXS modulates the Bcl-2/Beclin-1/LC3A/B pathway in VaD treatment.</div></div><div><h3>Results</h3><div>LC‒MS/MS identified 164 active ingredients in the KXS ethanol extract. In 2-vessel occlusion model rats, KXS significantly improved learning and memory (<em>p</em> &lt; 0.05 or <em>p</em> &lt; 0.01) and reduced hippocampal CA1 neuronal damage. Western blotting showed KXS upregulated Bcl-2/Bcl-XL and downregulated Beclin-1, LC3A/B, and Bax (<em>p</em> &lt; 0.05). Immunofluorescence confirmed increased Bcl-2 and decreased Beclin-1 expression. Network pharmacology predicted 73 targets and 174 pathways, with TNF, AKT1, IL-1β, PTGS2, and ESR1 as key targets. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis linked these targets to cancer, atherosclerosis, and AGE-RAGE signaling pathways.</div></div><div><h3>Conclusions</h3><div>KXS alleviates VaD by modulating autophagy and apoptosis via Bcl-2/Beclin-1/LC3A/B, with multi-target, multi-pathway effects. These findings support further investigation of KXS as a potential therapy for VaD.</div><div><strong>How to cite:</strong> Li M, Lu Y, Li Y, et al. Kaixin San in treating vascular dementia via regulating the Bcl-2/Beclin-1/LC3A/B signaling pathway via animal experiments and network pharmacology analysis. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.08.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 96-109"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145413009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating exosomal miRNA signatures as potential biomarkers and therapeutic targets in biliary colic","authors":"Xiangjie Han,&nbsp;Anshi Wu,&nbsp;Mengmeng Bao","doi":"10.1016/j.ejbt.2025.05.007","DOIUrl":"10.1016/j.ejbt.2025.05.007","url":null,"abstract":"<div><h3>Background</h3><div>Biliary colic (BC), characterized by intermittent pain due to gallstone-related bile duct obstruction, remains poorly understood at the molecular level. Circulating exosomal microRNAs (miRNAs) have emerged as potential biomarkers for various diseases. This study aimed to identify exosomal miRNA profiles in BC patients and explore their therapeutic implications.</div></div><div><h3>Results</h3><div>Analysis of plasma exosomal miRNAs from 10 BC patients during acute attacks and 10 healthy controls (HCs) revealed distinct expression patterns separating BC from HC groups. Integration of differential expression analysis, WGCNA, and LASSO regression identified 7 key miRNAs (hsa-miR-142-3p, hsa-miR-32-5p, hsa-miR-374a-3p, hsa-miR-155-5p, hsa-miR-425-3p, hsa-miR-584-5p, hsa-miR-185-5p) strongly associated with BC. Support vector machine models using these miRNAs achieved excellent diagnostic performance (AUC = 1.0, where AUC represents Area Under the Curve). miRNA-targeting drugs including Remlarsen and Cobomarsen showed potential for therapeutic intervention.</div></div><div><h3>Conclusions</h3><div>This study identified specific exosomal miRNA signatures that distinguish BC patients from HC and revealed potential miRNA-targeting therapeutics. These findings advance our understanding of BC pathophysiology and provide direction for developing novel diagnostics and treatments.</div><div><strong>How to cite:</strong> Han X, Wu A, Bao M. Circulating exosomal miRNA signatures as potential biomarkers and therapeutic targets in biliary colic. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.007</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 1-13"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and metabolomic profiling reveals key mechanisms of alkaline stress tolerance in rice","authors":"Jiangxu Wang ,&nbsp;Chuang Lang ,&nbsp;Yang Ren ,&nbsp;Junxiang Guo ,&nbsp;Wendong Ma ,&nbsp;Qing Liu ,&nbsp;Lei Lei ,&nbsp;Shichen Sun","doi":"10.1016/j.ejbt.2025.07.002","DOIUrl":"10.1016/j.ejbt.2025.07.002","url":null,"abstract":"<div><h3>Background</h3><div>Alkaline stress severely restricts rice growth and yield by disrupting ion balance, nutrient uptake, and oxidative metabolism. Clarifying the molecular mechanisms of tolerance is vital for breeding resilient varieties. This study explores transcriptional and metabolic adaptations in an alkali-tolerant (Qijing 10, LD) and sensitive (Tengxi 138, WL) rice variety under alkaline stress.</div></div><div><h3>Results</h3><div>Transcriptomic analysis revealed 1297 differentially expressed genes (DEGs) in the sensitive variety under alkaline stress (TWL), primarily enriched in pathways related to antioxidant enzyme synthesis (e.g., peroxidase genes), transmembrane ion transport, and membrane lipid stabilization pathways. In contrast, the tolerant variety (TLD) exhibited only 38 DEGs, suggesting transcriptional homeostasis achieved via suppression of stress-related gene overactivation. Metabolomic profiling demonstrated stable levels of key lipids (phosphatidic acid, galactolipids) and osmolytes (proline, betaine) in the tolerant variety under stress, whereas the sensitive variety accumulated lipid peroxidation products (malondialdehyde, MDA) and displayed dysregulated carbohydrate metabolic dysregulation. Integrated multi-omics analysis indicated that the tolerant variety coordinated lipid metabolism gene modulation with antioxidant metabolite accumulation, establishing dual barriers for ROS scavenging and membrane protection. Conversely, transcriptional dysregulation in the sensitive variety led to metabolic collapse.</div></div><div><h3>Conclusions</h3><div>Alkaline tolerance in rice hinges on the synergistic modulation of stress-responsive genes and metabolic networks to preserve redox equilibrium and membrane function. The tolerant variety’s capacity to stabilize transcriptional activity and metabolic flux underlies its resilience. These results elucidate key molecular and metabolic determinants of alkaline tolerance, offering strategic targets for breeding rice cultivars adapted to alkaline environments.</div><div><strong>How to cite:</strong> Wang J, Lang C, Ren Y, et al. Transcriptomic and metabolomic profiling reveals key mechanisms of alkaline stress tolerance in rice. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 35-45"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling microbial secondary metabolite biosynthetic gene clusters from alkaline soda Lake Chitu, in the Ethiopian Rift Valley","authors":"Gessesse Kebede Bekele ,&nbsp;Ermias Sissay Balcha ,&nbsp;Abu Feyisa Meka ,&nbsp;Eskedar Getachew Assefa ,&nbsp;Ebrahim M. Abda ,&nbsp;Fassil Assefa Tuji ,&nbsp;Mesfin Tafesse Gemeda","doi":"10.1016/j.ejbt.2025.06.002","DOIUrl":"10.1016/j.ejbt.2025.06.002","url":null,"abstract":"<div><h3>Background</h3><div>Microorganisms inhabiting alkalihalo-soda lakes are known for producing diverse secondary metabolites with potential biotechnological and pharmaceutical applications. This study explored the biosynthetic capabilities of microbial communities from Ethiopia’s Chitu Lake through shotgun metagenomic sequencing and metagenome-assembled genome (MAG) analyses using various bioinformatics tools.</div></div><div><h3>Results</h3><div>Analysis of MAGs using the Antibiotics and Secondary Metabolite Analysis Shell (antiSMASH) revealed 13 major types of biosynthetic gene clusters. The most abundant were terpene-precursors (32%) and terpene clusters (25%), followed by ribosomally synthesized and post-translationally modified peptides (9%) and nonribosomal peptide synthetases (7%). Other less common BGCs (5% each) included betalactone, ectoine, and Type I polyketide synthase, while rare types (2% each) comprised arylpolyene, hydrogen cyanide, phosphonate, ranthipeptide, and others. The Natural Product Domain Seeker (NaPDoS) detected ketosynthase domains linked to pharmaceutically important such as various fatty acid synthesis, modular and iterative domain classes, and condensation domain which is associated with L-amino acid coupling (LCL) domain class, such as those involved in syringomycin biosynthesis. In addition, bacteriocin analysis identified sactipeptides (56%) and lasso peptides (28%) as dominant types. Kyoto Encyclopedia of Genes and Genomes pathway analysis uncovered several secondary metabolite pathways including those for penicillin, cephalosporins, alkaloids, and phenazines. Rapid Annotation using Subsystem Technology further highlighted secondary metabolism pathways vital for microbial survival in Chitu Lake’s extreme environment.</div></div><div><h3>Conclusions</h3><div>The discovery of diverse biosynthetic gene cluster positions Chitu Lake as a valuable source of secondary metabolites, highlighting the biotechnological, industrial, pharmaceutical, agricultural and environmental potential of its extremophilic microbes and supporting further bioprospecting efforts.</div><div><strong>How to cite:</strong> Bekele GK, Balcha ES, Meka AF, et al. Unveiling microbial secondary metabolite biosynthetic gene clusters from alkaline soda Lake Chitu, in the Ethiopian Rift Valley. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.06.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 48-58"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal community dynamics and pathogenic risk in composting, vermicomposting and leachate systems amended with rabbit manure","authors":"Rocío del Pilar Serrano-Ramírez ,&nbsp;Adalberto Zenteno-Rojas ,&nbsp;Valentín Pérez-Hernández ,&nbsp;Odín Reyes-Vallejo ,&nbsp;Sugey Vásquez-Hernández ,&nbsp;Héctor Hiram Torres-Ventura ,&nbsp;Mayram Margarita González-Reyes ,&nbsp;Wilber Montejo-López ,&nbsp;Abumalé Cruz-Salomón ,&nbsp;Joaquín Adolfo Montes-Molina","doi":"10.1016/j.ejbt.2025.05.002","DOIUrl":"10.1016/j.ejbt.2025.05.002","url":null,"abstract":"<div><h3>Background</h3><div>Understanding the dynamics of fungal communities in composting and vermicomposting systems is essential for optimizing waste management practices and minimizing pathogen risks. For this reason, this study assessed the fungal community structure and potential pathogenic risks in composting, vermicomposting and leachate systems amended with rabbit manure, using Illumina’s MiSeq platform for internal transcribed spacer (ITS) sequencing and FUNGuild analysis.</div></div><div><h3>Results</h3><div>Phylotypes from Basidiomycota were predominant in all treatments, while the pathogenic genus <em>Microascus</em>, initially abundant (37%), showed a significant reduction to 5% and 7% following composting and vermicomposting, respectively, and to 1% and 13% in their leachates. Given <em>Microascus</em>’s association with human skin diseases, proper handling of organic waste is critical before its agricultural use. In contrast, the FUNGuild analysis revealed a high abundance of saprotrophic fungi such as <em>Aspergillus</em>, <em>Preussia, Botryotrichum</em>, and <em>Acremonium</em> known for producing enzymes that promote nutrient cycling and soil fertility.</div></div><div><h3>Conclusions</h3><div>These findings highlight the potential for vermicomposting to reduce pathogen risks while enhancing fungal-driven nutrient recycling, offering practical insights for sustainable agriculture and organic waste management.</div><div><strong>How to cite:</strong> Serrano-Ramírez RdP, Zenteno-Rojas A, Pérez-Hernández V, et al. Fungal community dynamics and pathogenic risk in composting, vermicomposting and leachate systems amended with rabbit manure. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 1-11"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge","authors":"Chutema Thongsongkaew ,&nbsp;Benjamas Cheirsilp ,&nbsp;Asma Billateh ,&nbsp;Wageeporn Maneechote ,&nbsp;Sirasit Srinuanpan","doi":"10.1016/j.ejbt.2025.05.005","DOIUrl":"10.1016/j.ejbt.2025.05.005","url":null,"abstract":"<div><h3>Background</h3><div>Aquaculture has become the fastest-growing sector in recent decades, and this has led to intensified cultural practices to achieve high yields. However, such practices have raised concerns regarding their environmental impact, as aquaculture sludge and wastewater cause significant organic pollution. This study then aimed to isolate and screen proteolytic, amylolytic, and lipolytic bacteria and evaluate their consortia for enzyme production and bioremediation of shrimp pond sludge.</div></div><div><h3>Results</h3><div>The strategy using multiple substrates in the isolation media successfully obtained bacterial strains with multiple hydrolytic activities. After primary and secondary quantitative screening, 18 isolates that exhibited high dual and triple hydrolytic activities were selected. After tertiary quantitative screening using synthetic shrimp pond sludge and co-culture tests, <em>Exiguobacterium indicum</em> SSP-PA-08, <em>Bacillus coagulans</em>, and <em>Bacillus subtilis</em> were selected due to their synergy for the production of triple hydrolytic enzymes. Their consortia inoculated in shrimp pond sludge containing 0.8% total suspended solids (TSSs) showed an increase in proteolytic activity by 2.5 folds and amylolytic activity by 20 folds, which led to a greater reduction in TSS. The highest enzyme production was obtained using shrimp pond sludge containing 1.6% TSS.</div></div><div><h3>Conclusions</h3><div>This study has developed the methods to isolate bacteria with multienzyme-producing ability. Co-culturing these bacteria in synthetic shrimp sludge significantly enhanced hydrolytic activity and led to a greater reduction in TSS. These strategies may contribute greatly to the hydrolytic enzyme production and environmentally friendly bioremediation of aquaculture sludge.</div><div><strong>How to cite:</strong> Thongsongkaew C, Cheirsilp B, Billateh A, et al. Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 35-47"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, purification and physicochemical characterization of Cichorium intybus L. root polysaccharide and its protective effect against CCl4-induced liver injury","authors":"Yingze Ma,&nbsp;Zhiping Yang,&nbsp;Weili Huang","doi":"10.1016/j.ejbt.2025.06.003","DOIUrl":"10.1016/j.ejbt.2025.06.003","url":null,"abstract":"<div><h3>Background</h3><div>Liver injury is a major cause of hepatic diseases, often leading to impaired liver function. Current treatments face limitations due to potential hepatotoxicity, driving interest in alternative therapies like Traditional Chinese Medicine (TCM). <em>Cichorium intybus</em> L., a medicinal herb rich in bioactive polysaccharides, has shown promise in liver protection.</div></div><div><h3>Results</h3><div>A water-soluble polysaccharide (CIP-N-1), with an average molecular weight of 2.3 kDa, was isolated and purified from the water extract of <em>Cichorium intybus</em> L. root using DEAE cellulose column chromatography and CL-6B agarose gel chromatography. CIP-N-1 consists of 98.16% neutral sugars and 1.69% proteins, with its primary components being mannose and glucose in a ratio of 4.9:95.1. In vivo studies demonstrated CIP-N-1′s hepatoprotective effects by enhancing antioxidant activity, inhibiting lipid peroxidation, and reducing inflammation in CCl₄-induced liver injury.</div></div><div><h3>Conclusions</h3><div>CIP-N-1 shows potential as a dietary supplement for alleviating chemical liver damage. Its antioxidant and anti-inflammatory properties support its use in liver health, offering a natural therapeutic option for hepatic injury prevention and treatment.</div><div><strong>How to cite:</strong> Ma Y, Yang Z, Huang W. Isolation, purification and physicochemical characterization of <em>Cichorium intybus</em> L. root polysaccharide and its protective effect against CCl4-induced liver injury. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.06.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 59-65"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144924951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onion peel-mediated biosynthesis of TiO2-ZnO bimetallic nanoparticles: Antimicrobial, antibiofilm, and anticancer activities","authors":"Ebrahim Saied ,&nbsp;Ahmed S. Doghish ,&nbsp;Mohamed K.Y. Soliman ,&nbsp;Walaa A. El-Dakroury ,&nbsp;Abeer S. Aloufi ,&nbsp;Bushra Hafeez Kiani ,&nbsp;Amr H. Hashem","doi":"10.1016/j.ejbt.2025.06.001","DOIUrl":"10.1016/j.ejbt.2025.06.001","url":null,"abstract":"<div><h3>Background</h3><div>The rising challenges of antibiotic resistance and cancer necessitate the development of sustainable, cost-effective, and multifunctional therapeutic agents. This study introduces a green synthesis approach for bimetallic nanoparticles (BNPs) using agro-waste materials.</div></div><div><h3>Results</h3><div>For the first time, bimetallic titanium dioxide–zinc oxide (TiO₂-ZnO) BNPs were synthesized using onion peel extract as a natural reducing and stabilizing agent. UV–Vis spectroscopy confirmed nanoparticle formation with a peak corresponding to a size of approximately 320 nm. DLS showed an average hydrodynamic diameter of 145.1 nm, and TEM revealed monodispersed nanoparticles, ranging from 80 to 150 nm. The BNPs exhibited broad-spectrum antimicrobial activity with MIC values of 500 μg/mL against <em>Bacillus subtilis</em>, <em>Pseudomonas aeruginosa</em>, and <em>Candida albicans</em>; 1000 μg/mL against <em>Staphylococcus aureus</em>; and 250 μg/mL against <em>Escherichia coli</em>. They also demonstrated significant antibiofilm activity against <em>B. subtilis</em>-MRSA with a 63.1% inhibition rate at 125 μg/mL. Additionally, TiO₂-ZnO BNPs showed potent cytotoxic effects on MCF-7 breast cancer cells, with an IC₅₀ of 5.97 ± 0.37 μg/mL, and anticancer activity was mediated by caspase-8 activation and VEGFR-2 downregulation.</div></div><div><h3>Conclusions</h3><div>This green-synthesized TiO₂-ZnO BNPs offer a promising dual-function nanoplatform for combating microbial infections and cancer, highlighting the potential of sustainable nanotechnology for biomedical applications.</div><div><strong>How to cite:</strong> Saied E, Doghish AS, Soliman MK, et al. Onion peel-mediated biosynthesis of TiO₂-ZnO bimetallic nanoparticles: Antimicrobial, antibiofilm, and anticancer activities. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.06.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 12-23"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}