Exploring the multifaceted bioactivities of silver nanoparticles synthesized from red algae Hypnea pannosa: Antimicrobial, antibiofilm, and insecticidal potentials

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology Pub Date : 2025-03-01 DOI:10.1016/j.ejbt.2024.12.001

Mansour A.E. Bashar , Enas M.H. Attia , Alsayed E. Mekky , Tharwat A. Selim , Walaa M. Shaban , Mohamed A.M. El-Tabakh , Ammar.M. Mahmoud , Mostafa A. Abdel-Maksoud , Ali A. Ali , Mohamed A. El-Tayeb , Waleed B. Suleiman , Mohamed E. El Beeh , Sabiha Fatima , Bushra Hafeez Kiani , Nehal M. Khairy , Ebrahim Saied

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引用次数: 0

Abstract

Background

The emergence of microbial resistance to conventional antibiotics and the environmental impact of chemical pesticides necessitates the search for alternative and sustainable solutions. This study explores the utilization of the dried biomass of the red algae Hypnea pannosa to synthesize silver nanoparticles (AgNPs) using a green and eco-friendly method.

Results

The synthesized AgNPs displayed sizes ranging from 15 to 60 nm with polydispersed shapes and a face-centered cubic crystalline structure, confirmed through characterization techniques including transmission electron microscopy (TEM), dynamic light scattering (DLS), and X-ray diffraction (XRD). Stability assessments via zeta potential measurements and docking studies of oleic acid and 9,12-octadecadienoic acid against Gyrase B and GST proteins were also conducted. The nanoparticles demonstrated potent antibacterial and antibiofilm activities, particularly against Staphylococcus epidermidis. Both the algal extract and the AgNPs exhibited significant larvicidal and adulticidal effects against Culex pipiens, with the nanoparticles showing superior efficacy, indicated by lower LC50 and LC90 values. The highest larvicidal effectiveness achieved was 93.33% for algal extract at 600 mg/L and 100% for AgNPs at 100 mg/L.

Conclusions

This study provides a sustainable method for producing AgNPs with diverse applications in medical and mosquito control fields, highlighting their potential as effective and safe alternatives to conventional antibacterial and insecticidal agents.

How to cite: Bashar MA, Attia EM, Mekky AE, et al. Exploring the multifaceted bioactivities of silver nanoparticles synthesized from red algae Hypnea pannosa: Antimicrobial, antibiofilm, and insecticidal potentials. Electron J Biotechnol 2025;74. https://doi.org/10.1016/j.ejbt.2024.12.001.

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查看原文本刊更多论文

探索由红藻合成的银纳米颗粒的多方面生物活性：抗菌、抗生物膜和杀虫潜力

微生物对常规抗生素的耐药性和化学农药对环境的影响的出现，要求寻找替代和可持续的解决方案。本研究探索了利用红藻的干燥生物量，利用绿色环保的方法合成纳米银粒子（AgNPs）。结果通过透射电子显微镜（TEM）、动态光散射（DLS）和x射线衍射（XRD）等表征技术证实，合成的AgNPs尺寸在15 ~ 60 nm之间，具有多分散形状和面心立方晶体结构。通过zeta电位测量和油酸和9,12-十八二烯酸对Gyrase B和GST蛋白的对接研究进行了稳定性评估。纳米颗粒表现出强大的抗菌和抗生物膜活性，特别是对表皮葡萄球菌。藻类提取物和AgNPs对淡库蚊均有显著的杀幼虫和杀成虫效果，其中纳米颗粒的LC50和LC90值较低，效果更佳。600 mg/L海藻提取物和100 mg/L AgNPs的最高杀虫率分别为93.33%和100%。结论本研究提供了一种可持续生产AgNPs的方法，在医疗和防蚊领域具有广泛的应用，突出了其作为传统抗菌和杀虫剂的有效和安全替代品的潜力。引用方式：Bashar MA， Attia EM， Mekky AE等。探索由红藻合成的银纳米颗粒的多方面生物活性：抗菌、抗生物膜和杀虫潜力。中国生物医学工程学报（英文版）；2009;34。https://doi.org/10.1016/j.ejbt.2024.12.001。

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering