Network pharmacology and experimental verification reveal the mechanism of Qingfei Tongluo mixture in treating pulmonary fibrosis

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology Pub Date : 2025-01-01 DOI:10.1016/j.ejbt.2024.10.002

Ying Zhou , Wenlong Wang , Wanping Zhu , Tingting Cai , Nannan Wang , Xia Liu , Wenmin Wang , Kequn Chai

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Abstract

Background

Pulmonary fibrosis (PF) is a chronic interstitial lung disease posing significant health risks. This study aimed to investigate the therapeutic mechanism of Qingfei Tongluo mixture (QTm) in treating PF by combining network pharmacology and experimental verification.

Results

A total of 246 active ingredients in QTm were identified, with 159 potential targets for PF treatment. Quercetin, a key active ingredient, was associated with the TGF-β1 signaling pathway. Gene Ontology and KEGG enrichment analyses identified 42 core genes, with a notable implication of the TGF-beta signaling pathway in PF. Immunohistochemistry showed elevated FTO and TGF-β1 levels in PF tissues. Animal experiments demonstrated that QTm improved alveolar structure, reduced interstitial lesions, and enhanced lung function while decreasing hydroxyproline content and the expression of FTO and TGF-β1 proteins.

Conclusions

QTm may inhibit PF progression by suppressing FTO/TGF-β1 expression, thereby improving lung function. These findings suggest that QTm holds potential as a treatment for PF.

How to cite: Zhou Y, Wang W, Zhu W, et al. Network pharmacology and experimental verification reveal the mechanism of Qingfei Tongluo mixture in treating pulmonary fibrosis. Electron J Biotechnol 2025;73. https://doi.org/10.1016/j.ejbt.2024.10.002.

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网络药理学和实验验证揭示清肺通络合剂治疗肺纤维化的作用机制

肺纤维化（PF）是一种慢性间质性肺疾病，具有重大的健康风险。本研究旨在通过网络药理学和实验验证相结合的方法，探讨清肺通络合剂治疗PF的作用机制。结果共鉴定出246种有效成分，其中潜在靶点159种。槲皮素是一种关键活性成分，与TGF-β1信号通路相关。基因本体和KEGG富集分析鉴定出42个核心基因，与PF中TGF-β信号通路相关，免疫组化显示PF组织中FTO和TGF-β1水平升高。动物实验表明，QTm改善肺泡结构，减少间质病变，增强肺功能，降低羟脯氨酸含量，降低FTO和TGF-β1蛋白的表达。结论sqtm可能通过抑制FTO/TGF-β1的表达来抑制PF的进展，从而改善肺功能。这些发现表明，QTm有可能作为一种治疗前列腺癌的方法。引用本文：周颖，王伟，朱伟等。网络药理学和实验验证揭示清肺通络合剂治疗肺纤维化的作用机制。中国生物医学工程学报（英文版）；2009;73。https://doi.org/10.1016/j.ejbt.2024.10.002。

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering