Diagnostic Cytopathology最新文献

{"title":"Recurrent Acinic Cell Carcinoma of Parotid Gland Diagnosed by Fine Needle Aspiration Cytology: A Rare Case Report","authors":"Anju Khairwa,&nbsp;Priya Suneja,&nbsp;Preeti Diwaker","doi":"10.1002/dc.25505","DOIUrl":"https://doi.org/10.1002/dc.25505","url":null,"abstract":"<div>\u0000 \u0000 <p>Acinic cell carcinoma (ACC) is a rare malignant tumor of the head and neck region. It accounts for 12.4%–17.4% of parotid gland malignancies and approximately 3.4% of all salivary gland tumors. ACC is a low-grade salivary gland malignancy that predominantly affects females. In some cases, ACC can undergo high-grade transformation (HGT), resulting in more aggressive behavior. Here, we present a rare case of recurrent Acinic cell carcinoma with aggressive features and high-grade cytomorphology—including increased cellularity, papillary and singly scattered cells, high nuclear-to-cytoplasmic (N:C) ratio, coarse chromatin, and single-cell necrosis in the background—diagnosed via fine-needle aspiration cytology (FNAC) and further confirmed by histopathology and immunohistochemistry. This case can be categorized as ACC recurrence associated with high-grade transformation (HGT).</p>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"E190-E193"},"PeriodicalIF":1.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytopathology in India: Past, Present, and Future","authors":"Jitendra Singh Nigam,&nbsp;Jyotsna Naresh Bharti,&nbsp;Ashutosh Rath,&nbsp;Immanuel Pradeep,&nbsp;Biswajit Dey,&nbsp;Ravi Mehrotra","doi":"10.1002/dc.25503","DOIUrl":"10.1002/dc.25503","url":null,"abstract":"<div>\u0000 \u0000 <p>Cytopathology was apparent as a feasible and inexpensive screening and diagnostic technique incorporated in India during the 1950s and 1960s. The Indian Academy of Cytologists (IAC) was established to foster cytology research, standardize nomenclature, and advance cytology across India. Although cytopathology is a fundamental component of the postgraduate pathology curriculum, numerous challenges and limitations impede its practice in India, including a shortage of trained cytopathologists, uneven distribution of cytopathology services and their quality assurance, and financial constraints for advanced diagnostics in cytopathology samples. These obstacles hinder the efficient execution of cytopathology activities in India. This review outlines the historical evolution, expansion, institutional and academic advancements, cytology-based screening programs and guidelines, quality assurance, research output, and contemporary developments in cytopathology reporting, as well as the challenges and limitations encountered by cytopathology practice in India. The review argues for intentional initiatives to augment the capability of cytopathology in remote and rural regions of India, facilitating early and improved cancer detection and patient outcomes.</p>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 9","pages":"466-470"},"PeriodicalIF":1.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Uterine Manipulator on Peritoneal Cytology Positivity in Endometrial Cancer: A Prospective Observational Study","authors":"Ceren Gerçek,&nbsp;Hande Nur Öncü,&nbsp;Tuğba Taşkın Türkmenoğlu,&nbsp;Candost Hanedan,&nbsp;Vakkas Korkmaz","doi":"10.1002/dc.25497","DOIUrl":"10.1002/dc.25497","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>There are studies suggesting that the use of a uterine manipulator during minimally invasive surgery (MIS) may lead to tumor spread in endometrial cancer patients, and there is no clear consensus on the safety of uterine manipulator use. The aim of this study was to investigate the effect of uterine manipulator use on peritoneal cytology (PC) results obtained before and after hysterectomy in endometrial cancer patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A single-center, prospective observational study was conducted, including 108 patients who were diagnosed with endometrial cancer and underwent surgical intervention. The patients were divided into three groups: Group 1 (<i>n</i> = 36), total abdominal hysterectomy (TAH); Group 2 (<i>n</i> = 41) laparoscopic hysterectomy with intrauterine manipulator (TLH with IUM); and Group 3 (<i>n</i> = 31) laparoscopic hysterectomy without IUM (TLH without IUM). PC samples were taken before and after the hysterectomy, and cytology results were compared among the groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of the 108 patients included in the study, 33.3% (36/108) were in Group 1, 38% (41/108) were in Group 2, and 28.7% (31/108) were in Group 3. The clinical and demographic data among the groups were similar (<i>p</i> &gt; 0.05). PC was positive in six of the 108 patients. In the TLH with IUM group, one patient with a negative cytology sample before the manipulation showed a positive result after the hysterectomy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Although statistical significance was not reached, utilizing an IUM to manage endometrial cancer with MIS may enhance cytology positivity. This finding requires further validation through larger prospective studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"494-500"},"PeriodicalIF":1.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroblastoma in Pregnancy: Expanding the Cytomorphological Spectrum of a Novel DICER1-Associated Entity, a Case Report and Literature Review","authors":"R. Razack,&nbsp;A. C. van Wyk,&nbsp;P. T. Schubert,&nbsp;D. Pelletier,&nbsp;W. D. Foulkes,&nbsp;C. Njovu,&nbsp;W. Conradie,&nbsp;F. Hoosain","doi":"10.1002/dc.25504","DOIUrl":"https://doi.org/10.1002/dc.25504","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Thyroblastoma is a rare, aggressive thyroid neoplasm newly classified in the 2022 WHO Classification of Endocrine Tumors. It is characterized by embryonal, multilineage morphology and <i>DICER1</i> mutations. Fewer than 15 well-characterized cases have been reported, with limited cytological descriptions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Case Presentation</h3>\u0000 \u0000 <p>A 19-year-old pregnant woman presented with a rapidly enlarging right thyroid mass. Fine needle aspiration biopsy (FNAB) revealed a highly cellular, triphasic aspirate composed of primitive epithelium arranged in macrofollicular structures, spindled mesenchymal cells within a myxoid matrix, and small round blastemal cells. Immunocytochemistry showed TTF-1 and PAX8 positivity in epithelial cells, synaptophysin and SALL4 in blastemal cells, and myogenin in both spindled and blastemal components, supporting a cytological diagnosis of thyroblastoma. She underwent total thyroidectomy and neck dissection during the second trimester. Histology confirmed thyroblastoma with extensive lymph node metastases. Chemotherapy was initiated during pregnancy but discontinued due to neutropenic complications. She delivered a healthy infant and remained disease-free at her 12-month follow-up.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Molecular Findings</h3>\u0000 \u0000 <p>Next-generation sequencing of tumor DNA revealed two somatic <i>DICER1</i> mutations: a known hotspot missense mutation, c.5437G&gt;C; p.E1813Q, and a novel splice-site variant, c.734+1G&gt;T, predicted to disrupt normal splicing and protein function. No pathogenic variants were found in germline DNA, supporting a somatic origin.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This case expands the cytological and molecular spectrum of thyroblastoma and highlights the value of FNAB in early recognition. Awareness of this rare entity and its diagnostic features is essential to avoid misclassification and ensure timely management. The novel <i>DICER1</i> splice-site mutation further contributes to the evolving molecular landscape of thyroblastoma.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"E181-E189"},"PeriodicalIF":1.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dc.25504","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Panel Testing of BRAF Mutation, RAS Mutation, and RET/PTC Rearrangements or RET Mutation Using Fine-Needle Aspiration Specimens in Evaluation of Thyroid Carcinoma","authors":"Zhaohui Zhu,&nbsp;Hailei Tong,&nbsp;Xu Qian,&nbsp;Meiling Xue,&nbsp;Aijun Sun,&nbsp;Yifei Yin,&nbsp;Weimin Sun,&nbsp;Ling Mao","doi":"10.1002/dc.25500","DOIUrl":"10.1002/dc.25500","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Thyroid carcinoma (TC) is a common malignancy of the endocrine system. This research investigates the value of multiple gene detection in evaluating the pathological features of TC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This retrospective study includes samples from 213 participants who underwent fine-needle aspiration, among whom 176 were diagnosed as TC and 37 with benign lesions. All patients underwent polygenic (BRAF, RAS, and RET) mutation detection. The surgical and pathological results were used as the gold standard to analyze the correlation between different gene mutations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The diagnostic accuracy and sensitivity of multigene panel testing were remarkably superior to those of BRAF, RAS, and RET alone, whereas the omission diagnosis rate was lower than that of BRAF, RAS, and RET alone. The diagnostic area under the curve of BRAF, RAS, RET, and combined detection for TC was 0.8871, 0.5468, 0.5717, and 0.9396, respectively; RAS and RET gene mutation was closely related to the lymph node metastasis of the enrolled patients (<i>p</i> &lt; 0.05). Mutation status for both single-gene testing and multigene panel testing closely correlated with the histological subtype of the patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>While BRAF mutations are associated with papillary TC, RAS mutations are more common in follicular TC, and RET mutations are common in medullary TC. Combined detection of RAS and RET gene mutations in TC has a certain correlation with the disease's pathological characteristics, which provides new ideas and measures for the subsequent diagnosis and evaluation of TC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"485-493"},"PeriodicalIF":1.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Pitfalls in Fine Needle Aspiration of Pleomorphic Adenoma: Degenerative Changes in Myoepithelial Cells as Malignant and PLAG1 False Negativity","authors":"Xiaobing Jin,&nbsp;Jonathan B. McHugh,&nbsp;Amer Heider","doi":"10.1002/dc.25502","DOIUrl":"https://doi.org/10.1002/dc.25502","url":null,"abstract":"<p>Fine needle aspiration (FNA) is widely accepted as an efficient first-line diagnostic tool for evaluating salivary gland lesions. It can accurately diagnose a majority of common benign tumors, including pleomorphic adenoma (PA). However, PA can exhibit a wide range of morphological variations, which may lead to misinterpretation. Here, we present a case in which degenerative myoepithelial cells with nuclear atypia in PA were misinterpreted as malignant. Furthermore, PLAG1 false negativity in cell block material compounded the diagnostic challenge. This report aims to highlight this diagnostic pitfall and alert cytopathologists by providing an illustrative example.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 9","pages":"E176-E180"},"PeriodicalIF":1.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dc.25502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targetable Molecular Profiles in Salivary Duct Carcinoma: A Review and Case Study of Molecular Diagnostic and Therapeutic Implications.","authors":"Gloria Hopkins Sura","doi":"10.1002/dc.25501","DOIUrl":"https://doi.org/10.1002/dc.25501","url":null,"abstract":"<p><p>Salivary duct carcinoma is a rare, high-grade malignancy of the salivary glands, most commonly arising in the parotid. This review highlights recent advancements in the cytologic, immunophenotypic, and molecular characterization of salivary duct carcinoma, emphasizing the central role of biomarkers such as androgen receptor (AR) overexpression and ERBB2 (HER2) amplification in diagnosis, prognosis, and therapeutic decision-making. These principles are illustrated through the case of a 55-year-old male with AR-positive, HER2-amplified salivary duct carcinoma of the right parotid gland with nodal and osseous metastases. Diagnosis was established via fine-needle aspiration (FNA) cytology, immunocytochemistry, and molecular profiling. The patient underwent androgen deprivation therapy followed by treatment with a HER2 targeted antibody-drug conjugate, achieving disease stability. This case exemplifies how molecular diagnostics inform personalized treatment strategies in salivary duct carcinoma and underscores the need for standardized testing protocols and greater awareness of precision oncology approaches in salivary gland malignancies.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological Assessment of Metastatic Gastrointestinal Stromal Tumor in Ascitic Fluid","authors":"Hadi Sultan,&nbsp;Zohre Sadeghian,&nbsp;Elvia Goez-Gutierrez,&nbsp;M. Julia Diacovo,&nbsp;Esther Yoon","doi":"10.1002/dc.25499","DOIUrl":"10.1002/dc.25499","url":null,"abstract":"<p>Gastrointestinal stromal tumors (GISTs) are mesenchymal neoplasms typically arising in the stomach and small intestine and rarely involving extra-gastrointestinal organs. While fine-needle aspiration (FNA) offers reliable diagnosis, exfoliation of GIST cells into body fluids is exceedingly rare and diagnostically challenging. We report a case of a 69-year-old male with metastatic GIST in ascitic fluid. The cells revealed atypical epithelioid morphology with high nuclear-to-cytoplasmic ratio and nuclear irregularities. Immunohistochemistry (IHC) demonstrated positivity for CD117 and DOG1. Diagnosis requires integration of clinical history, cytomorphology, and a broad IHC panel. Awareness of its cytologic features, application of mesenchymal markers, and molecular testing are critical for accurate diagnosis and effective clinical management.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 9","pages":"462-465"},"PeriodicalIF":1.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dc.25499","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BAP1 Loss on Immunocytochemistry and Its Association With Monosomy 3 in Uveal Melanoma Fine-Needle Aspirations","authors":"Precious Ann Fortes,&nbsp;Michelle Nguyen,&nbsp;Po Chu Fung,&nbsp;Erika F. Rodriguez,&nbsp;Teresa H. Kim,&nbsp;Kosuke Kawai,&nbsp;Neda A. Moatamed","doi":"10.1002/dc.25496","DOIUrl":"10.1002/dc.25496","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Loss of nuclear BRCA1-associated protein 1 (nBAP1) expression is strongly linked to monosomy 3 in uveal melanoma. While fine-needle aspiration (FNA) aids diagnosis, the prognostic value of nBAP1 immunocytochemistry (ICC) is still being investigated. This study examines the correlation between nBAP1 loss on ICC and fluorescence in situ hybridization (FISH) findings, as well as its clinical impact.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Intraocular FNA cytology specimens with clinical concern for uveal melanoma from April 2015 to March 2023 with available nBAP1 ICC, FISH results, and clinical follow-up were examined. Two independent reviewers, blinded to the cytogenetic results, interpreted the nBAP1 ICC as either loss or retained. Statistical analysis using Fisher's exact test was utilized to evaluate the relationship between nBAP1 loss on ICC and FISH findings. Kaplan–Meier survival plots were constructed to examine the metastasis-free survival.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among the 79 cases included in the study, 86.1% (68/79) showed nBAP1 loss. Approximately 63.2% of patients with nBAP1 loss had monosomy 3, whereas none with nBAP1 retained had monosomy 3 (<i>p</i> &lt; 0.001). Of the nBAP1 loss cases, 41.2% (28/68) had monosomy 3 alone, while 22.1% (15/68) had both monosomy 3 and 6p gain. Patients with nBAP1 loss had a significantly higher risk of metastasis (<i>p</i> ≤ 0.04), with a 5-year metastasis-free survival of 57.1% versus 100% in nBAP1 retained cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>BAP1 ICC is a cost-effective prognostic tool in uveal melanoma FNAs, strongly correlating with monosomy 3 and poor outcomes. Integrating BAP1 ICC with FISH enhances risk stratification, enabling earlier identification of high-risk patients and guiding treatment decisions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"476-484"},"PeriodicalIF":1.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Spectrum of Polyoma Virus and Polyoma Virus-Like Changes in Urine Cytology: When Should an Atypical Diagnosis Be Considered","authors":"Yaogong Li,&nbsp;Yonca Kanber,&nbsp;Derin Caglar,&nbsp;Wassim Kassouf,&nbsp;Manon Auger,&nbsp;Fadi Brimo","doi":"10.1002/dc.25494","DOIUrl":"10.1002/dc.25494","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>While the Paris system for reporting urinary cytology states that cells with well-recognized and typical changes of polyoma virus (PV) infection should not lead to a diagnosis of ‘atypia, the prognostic significance of PV-like features is unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Included were 284 urine cytology cases with PV or PV-like changes. Four cell types were identified. Cell A was typical of PV infection, cell B had a spider-web chromatin pattern, and cells C and D were degenerated cells with clumpy chromatin or homogeneous severely hyperchromatic nuclei, respectively. Events were correlated with a subsequent histological diagnosis of high-grade urothelial carcinoma (HGUC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>47% of cases had more than one cell type. The most common cell was D (64%) and the least common was A (27%). The most common cell to be present in isolation was D (<i>n</i> = 109, 60%). 92% of cases with cell A had other cell types. Overall, the presence of cell A was associated with a benign follow-up (1.2% association with HGUC). There was a gradual increase in the association with a HGUC diagnosis in cases with cells B (PPV = 8%), C (PPV = 15.5%), and D (PPV = 44%). The presence of only cells B, C, or D was predictive of HGUC in 22.4%, 33.3%, and 61.5%, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Cases with cell type A can be confidently diagnosed as ‘negative for HGUC’, even in the presence of associated cells with PV-like changes. Cases with cells displaying PV-like features (cell type B, C, or D) in the absence of type A features may represent degenerated HGUC cells.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 9","pages":"447-453"},"PeriodicalIF":1.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dc.25494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}