Diagnostic Cytopathology最新文献

{"title":"Alveolar Macrophages Mimic Tumor Cells in Lung Tissue: A Diagnostic Pitfall in Cytology FNA and EBUS Specimens","authors":"Joshua Maciejewski,&nbsp;Farnaz Hasteh,&nbsp;Charmi Patel,&nbsp;Maryam Pezhouh,&nbsp;Jingjing Hu","doi":"10.1002/dc.70010","DOIUrl":"10.1002/dc.70010","url":null,"abstract":"<div>\u0000 \u0000 <p>Fine-needle aspiration (FNA) or core needle biopsy via EBUS procedure is commonly used to assess suspicious lung nodules. Alveolar macrophages are commonly seen in these specimens. In cases when hypercellular specimens with large aggregates of alveolar macrophages are encountered, especially when they show epithelioid morphology, vacuolated cytoplasm, and significant cytological atypia, including marked anisonucleosis and intracytoplasmic inclusion, they can be mistakenly interpreted as “lesional cells” during rapid on-site evaluation (ROSE) and cease the EBUS procedure prematurely. It is important to keep in mind that epithelioid alveolar macrophages may mimic lung neuroendocrine tumor (NET), lung adenocarcinoma, or even metastatic process. Cytoplasmic pigmentation and immunohistochemistry analysis can be extremely useful to prevent a false diagnosis of malignancy.</p>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 11","pages":"E215-E220"},"PeriodicalIF":1.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Milan System SUMP Category: 5-Year Diagnostic Performance","authors":"Lester J. Layfield,&nbsp;Zubair Baloch","doi":"10.1002/dc.70002","DOIUrl":"10.1002/dc.70002","url":null,"abstract":"","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"473-475"},"PeriodicalIF":1.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometrial Liquid-Based Cytology Specimens Preserve High Genome Quality for Molecular Classification After Long-Term Storage","authors":"Toshiaki Akahane,&nbsp;Ikumi Kitazono,&nbsp;Seiya Yokoyama,&nbsp;Yuriko Higashi,&nbsp;Hiroaki Kobayashi,&nbsp;Akihide Tanimoto","doi":"10.1002/dc.70008","DOIUrl":"10.1002/dc.70008","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Molecular classification of endometrial cancer is useful for predicting prognosis. Genomic examinations are performed using formalin-fixed paraffin-embedded (FFPE) tissues; however, we previously reported that liquid-based cytology (LBC) specimens can be used for next-generation sequencing (NGS). In this study, we evaluated long-term storage effects of LBC specimens on NGS-based genomic profiling, including gene mutations, tumor mutation burden (TMB), and microsatellite instability (MSI).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Four LBC fixatives (CellPrep, ThinPrep, CytoRich Red, and SurePath) were used to prepare NGS samples from cultured endometrioid carcinoma HEK-251 cells. Twelve endometrial LBC specimens from patients with endometrioid carcinoma were fixed with CytoRich Red. The TMB, MSI, and gene mutations were analyzed after 1 week, 6 months, and 12 months of storage in cultured HEK-251 cells. Paired LBC and FFPE specimens of endometrioid carcinoma stored for 15–45 months were subjected to NGS-based analysis, and their molecular profiles were compared to those at the initial diagnosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The TMB and MSI did not differ during the storage periods for any of the LBC fixatives in the cultured cells; in addition to gene mutations, they were comparable between the initial and second analyses of the clinical FFPE and LBC specimens. There were no changes in the integrative diagnosis of the endometrioid carcinoma subtype classification.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>LBC specimens, which preserved high-quality genomes for molecular classification after long-term storage, may be an alternative source of genomic examination for the integrative diagnosis of endometrial cancer.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 11","pages":"536-545"},"PeriodicalIF":1.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue Acquisition Techniques Take Center Stage: Discussion Synopsis of Papanicolaou Society of Cytology Companion Session at International Academy of Cytology Congress, Florence, Italy","authors":"Zubair Baloch,&nbsp;Swikrity U. Baskota,&nbsp;William Faquin,&nbsp;Sara Monaco,&nbsp;Aziza Nassar","doi":"10.1002/dc.70003","DOIUrl":"10.1002/dc.70003","url":null,"abstract":"","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 11","pages":"521-528"},"PeriodicalIF":1.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144845006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study on the Impact of HPV Genotype Infection Patterns on the Occurrence of High-Grade Squamous Intraepithelial Lesion","authors":"Qiao Zhou,&nbsp;Kekai Luo,&nbsp;Liangping Zhao","doi":"10.1002/dc.70005","DOIUrl":"10.1002/dc.70005","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To investigate the differential risk of high-grade squamous intraepithelial lesion or worse (HSIL+) associated with single versus co-infection patterns of high-risk human papillomavirus (HR-HPV) genotypes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this retrospective cohort study, 10,570 patients with abnormal ThinPrep cytology test results and/or HR-HPV infection who underwent colposcopy-guided cervical biopsy at Wuhan Children's Hospital (May 2021–May 2023) were enrolled. Histopathological diagnosis served as the gold standard. Multivariate logistic regression was used to analyze HSIL+ risk across HPV infection patterns, adjusting for age and viral load.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Single infections with HPV31, HPV33, or HPV58 demonstrate comparable positivity rates of HSIL+ to HPV16 monoinfection. After adjusting for confounders, logistic regression revealed that co-infection of HPV16 with low-risk HPV genotypes reduced the risk of progression to HSIL+ compared to HPV16 monoinfection (<i>p</i> &lt; 0.05). Similarly, co-infections involving HPV33 or HPV58 (regardless of high/low-risk partners) were associated with lower HSIL+ risk (all <i>p</i> &lt; 0.05). In contrast, HPV31 demonstrated consistent HSIL+ risk irrespective of co-infection status.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>HPV16, HPV31, HPV33, or HPV58 need equivalent clinical vigilance in screening and management protocols. Co-infection with low-risk HPV genotypes attenuates HSIL+ risk in HPV16-infected individuals, and HPV33/58 co-infections (with any genotype) exhibit protective effects. Our study suggests that HPV31-associated risk might remain unaffected by co-infection, suggesting genotype-specific biological interactions. These findings highlight the importance of genotyping-guided risk stratification in cervical cancer screening.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 11","pages":"529-535"},"PeriodicalIF":1.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spiradenocylindroma: Morphology of a Rare Hybrid Adnexal Tumor","authors":"Sudha Sharma,&nbsp;Vasudha Sharma,&nbsp;Shailendra Kaushik","doi":"10.1002/dc.70006","DOIUrl":"10.1002/dc.70006","url":null,"abstract":"<div>\u0000 \u0000 <p>Spiradenocylindroma is a rare benign adnexal tumor with features of both spiradenoma and cylindroma, the scalp and face being the most common sites. They are considered to arise from pluripotent cells of abortive adnexal primordial tissue. Fine needle aspiration cytology, along with cell block, can give clues regarding the diagnosis. The final diagnosis is made on histopathology, which shows features of both cylindroma and spiradenoma in the same tumor. Multiple spiradenocylindromas can be associated with Brooke–Spiegler syndrome, where malignant transformation has also been reported. We report a case of spiradenocylindroma of the arm in a 50-year- old male and describe the clinical, cytologic, cell block, and histopathologic features.</p>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 11","pages":"E203-E209"},"PeriodicalIF":1.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-Needle Aspiration Cytologic Diagnosis of Intrathyroidal Thymic Carcinoma: A Review of the Literature","authors":"Qi Chen,&nbsp;Xiaoli Yu","doi":"10.1002/dc.70004","DOIUrl":"10.1002/dc.70004","url":null,"abstract":"<div>\u0000 \u0000 <p>Intrathyroidal thymic carcinoma is a rare thyroid carcinoma characterized by thymic epithelial differentiation. Given its relatively favorable prognosis, it is essential to differentiate it preoperatively from other more aggressive tumors. Fine-needle aspiration is widely used for the preoperative diagnosis of thyroid nodules. However, there is limited information on the cytological diagnosis, and its cytomorphological features remain poorly characterized. We perform a literature review of intrathyroidal thymic carcinoma and briefly describe two cases to further characterize its cytological features.</p>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"507-515"},"PeriodicalIF":1.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144834423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine Needle Aspiration Cytology of Metastatic Endolymphatic Sac Tumor: A Case Report","authors":"Daniel W. Masters,&nbsp;Jan F. Silverman","doi":"10.1002/dc.70001","DOIUrl":"https://doi.org/10.1002/dc.70001","url":null,"abstract":"<div>\u0000 \u0000 <p>Endolymphatic sac tumors are uncommon, slow growing, and locally aggressive neoplasms that arise within the endolymphatic sac, most commonly the intraosseous portion located in the temporal bone. There are only three previous cytology reports, and no prior FNAs have been reported. We present a case of a metastatic sporadic endolymphatic sac tumor, a phenomenon that has yet to be described on FNA and has only been previously reported once in cerebrospinal fluid cytology. We described the cytologic features on fine needle aspiration cytology and correlated the cytologic findings with the follow-up surgical resection of the metastatic focus.</p>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"E199-E202"},"PeriodicalIF":1.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytomorphological Features of Fine Needle Aspiration in 11 Conventional and 2 Dedifferentiated Chordomas: Correlation With Histopathology","authors":"Eva Manuela Pena-Burgos,&nbsp;Pilar López-Ferrer,&nbsp;Jose Juan Pozo-Kreilinger,&nbsp;Ana Margarita Rodríguez-García","doi":"10.1002/dc.70000","DOIUrl":"https://doi.org/10.1002/dc.70000","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chordoma is a rare malignant tumor of notochordal origin with well-established histologic features and typically distinctive cytomorphology. Fine-needle aspiration (FNA) can offer a valuable diagnostic tool in deep-seated or challenging lesions. However, distinguishing conventional and dedifferentiated chordomas based on cytological features remains difficult and poorly documented.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Thirteen FNA samples from 10 patients with histologically confirmed chordoma (11 conventional and 2 dedifferentiated), retrieved from the pathology files of a tertiary referral hospital between 1966 and 2022, were retrospectively reviewed. Cytomorphological features were analyzed and correlated with histological subtype and immunohistochemical profile.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All cases showed physaliphorous cells embedded in a myxoid matrix, with varying proportions of single cells and clusters. Prominent nucleoli, binucleation, and nuclear pseudoinclusions were frequently observed. Cytologic atypia and pleomorphism were notable in the dedifferentiated cases but also present, to a lesser extent, in some conventional chordomas. No definitive cytological features of sarcomatous transformation were identified in the smears of dedifferentiated chordoma. Immunohistochemistry supported the diagnosis in selected cases, with positivity for brachyury, cytokeratins, EMA, and negativity for S100 in the physaliphorous cells. Brachyury loss in dedifferentiated areas complicates diagnosis, underscoring the need for conventional component identification.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Chordoma shows distinctive yet variable cytomorphological features that can allow for a reliable diagnosis in FNA samples. Clinical and radiological correlation is essential to guide sampling, especially in the dedifferentiated cases, and avoid misinterpretation. Some cytological features typically associated with dedifferentiation may be present in conventional chordomas, underscoring the importance of cautious interpretation and histological confirmation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"E194-E198"},"PeriodicalIF":1.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Application Value of Colloidal Gold Immunochromatography Assay Combined With Fine Needle Aspiration Biopsy for Identifying Lymph Node Metastasis in Papillary Thyroid Carcinoma","authors":"Mingfeng Mao,&nbsp;Binqian Zhou,&nbsp;Xuejing Zhang,&nbsp;Xinjia Liu,&nbsp;Hongling Li,&nbsp;Jiaming Zhang","doi":"10.1002/dc.25498","DOIUrl":"10.1002/dc.25498","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>To investigate the accuracy of a colloidal gold immunochromatography assay (GICA) for thyroglobulin detection and evaluate the diagnostic performance of the combined GICA and fine-needle aspiration biopsy (FNAB) methods in identifying lymph node metastasis among patients with papillary thyroid carcinoma (PTC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Out of 97 PTC patients with suspicious cervical lymph nodes determined by the postoperative pathological diagnosis or the ultrasound-directed biopsy were chosen between January 2021 and October 2023. A total of 104 suspicious lymph nodes were detected via the GICA methods and the combined GICA and FNAB methods, respectively. The histological results were used as the gold standard. The accuracy and diagnostic performance were analyzed on the basis of the receiver operating characteristic curves.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among the 104 lymph nodes, 85 were positive and 19 were negative. For GICA methods, 47.92 ng/mL was the optimal cutoff value with a sensitivity of 93.98% and a specificity of 90.48% in the diagnosis of lymph node metastases. The accuracy of FNAB was 88.46%. The combined GICA and FNAB approach yielded an accuracy of 95.19%, a sensitivity of 96.39%, and a specificity of 90.48%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>GICA methods appeared to have high sensitivity and specificity for quickly diagnosing suspicious lymph nodes. The combined application of GICA and FNAB methods could improve the accuracy of the preoperative diagnosis and performed exceptionally well in diagnostic performance, providing a new convenient pathway for identifying lymph node metastases in clinical settings.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 10","pages":"501-506"},"PeriodicalIF":1.0,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}