Diagnostic Cytopathology最新文献

The Role of Fine Needle Aspiration Biopsy and Rapid Onsite Evaluation in the Diagnosis of Kidney Lesions. 细针穿刺活检和快速现场评估在肾脏病变诊断中的作用。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-21 DOI: 10.1002/dc.70025

Zehra Şahin, Şenay Erdoğan Durmuş, Deniz Ünlüer Kapışkay, Ahmet Baş, Nesrin Uygun

{"title":"The Role of Fine Needle Aspiration Biopsy and Rapid Onsite Evaluation in the Diagnosis of Kidney Lesions.","authors":"Zehra Şahin, Şenay Erdoğan Durmuş, Deniz Ünlüer Kapışkay, Ahmet Baş, Nesrin Uygun","doi":"10.1002/dc.70025","DOIUrl":"https://doi.org/10.1002/dc.70025","url":null,"abstract":"Background: Although core needle biopsy is widely preferred for evaluating renal lesions, fine needle aspiration (FNA) combined with rapid on-site evaluation (ROSE) remains a valuable diagnostic method in selected cases. This study aims to assess the diagnostic efficacy of renal FNA and highlight the impact of ROSE on achieving definitive diagnoses.Methods: This retrospective study included 273 patients who underwent ultrasound- or CT-guided FNA for renal lesions between 2010 and 2018 at Istanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine. Lesions were evaluated based on size, radiologic features, localization, ROSE status, and number of smears. Cytopathological results were categorized as nondiagnostic, limited, non-neoplastic, or neoplastic. Histopathological correlation was assessed in available cases. Statistical analyses were performed using SPSS v25.0, with p < 0.05 considered significant.Results: Of the 273 cases, 218 (79.8%) yielded diagnostic results. Definitive diagnosis rates increased with lesion size and were significantly higher in solid (86.3%) and mixed (91.4%) lesions compared to cystic lesions (60%) (p = 0.001). ROSE was performed in 177 cases, significantly improving diagnostic yield compared to cases without ROSE (85.8% vs. 68.7%, p = 0.001). Histopathological follow-up was available in 119 cases, with an overall cytological-histological concordance of 84%, reaching 98% in cases with adequate material.Conclusion: Renal FNA remains a reliable and accurate diagnostic method when combined with ROSE, especially in well-sampled cases. It aids in identifying both primary and metastatic tumors and can guide clinical decision-making, particularly when surgery is not feasible.","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosing Thyroid Lesions Through A Morphology-Molecular Lens: Four Case-Based Lessons From 22nd International Congress of Cytology-Florence 2025. 通过形态学-分子透镜诊断甲状腺病变：来自第二十二届佛罗伦萨国际细胞学大会的四个基于病例的经验教训。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-19 DOI: 10.1002/dc.70022

Sule Canberk, N Paul Ohori, Rita Luis, Elena Vigliar, Vivian Weiss, Zubair Baloch

{"title":"Diagnosing Thyroid Lesions Through A Morphology-Molecular Lens: Four Case-Based Lessons From 22nd International Congress of Cytology-Florence 2025.","authors":"Sule Canberk, N Paul Ohori, Rita Luis, Elena Vigliar, Vivian Weiss, Zubair Baloch","doi":"10.1002/dc.70022","DOIUrl":"https://doi.org/10.1002/dc.70022","url":null,"abstract":"Imaging-driven detection of thyroid nodules has expanded the role of fine-needle aspiration (FNA) while demanding integration of molecular data into routine cytologic assessment. We present four illustrative cases spanning the contemporary diagnostic spectrum: (1) an Atypia of Undetermined Significance (AUS) nodule with dual low-level PTEN mutations managed by active surveillance; (2) an oncocytic follicular neoplasm upgraded to total thyroidectomy by concurrent HRAS and TERT promoter mutations; (3) an oncocytic subtype poorly differentiated thyroid carcinoma (O-PDTC) highlighting subtle high-grade cytologic cues and a distinct genomic profile; and (4) a pediatric poorly differentiated carcinoma harboring a DICER1 hotspot mutation, underscoring age-specific biology and the need for genetic consideration. These cases emphasize that morphology remains foundational, but mutation context, single versus co-alterations, allelic burden, and patient age ultimately direct management. Harmonized reporting that clearly conveys molecular findings is essential to translate limited cytology material into precise, patient-specific care.","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and Pitfalls to Diagnosing NUTM1-Rearranged Neoplasia of the Pancreas by Cytology and Ancillary Studies. 细胞学和辅助研究诊断胰腺nutm1重排瘤的挑战和缺陷。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-18 DOI: 10.1002/dc.70021

Terrance J Lynn

引用次数: 0

The Spectrum of Malignant Diagnoses in Cerebrospinal Fluid (CSF) Cytology in Both Pediatric and Adult Populations: A Single-Institutional Retrospective Review. 儿童和成人人群脑脊液（CSF）细胞学恶性诊断谱：一项单机构回顾性评价。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-18 DOI: 10.1002/dc.70020

Nida Babar, Asif Loya, Sajid Mushtaq, Maryam Hameed, Usman Hassan, Mudassar Hussain

{"title":"The Spectrum of Malignant Diagnoses in Cerebrospinal Fluid (CSF) Cytology in Both Pediatric and Adult Populations: A Single-Institutional Retrospective Review.","authors":"Nida Babar, Asif Loya, Sajid Mushtaq, Maryam Hameed, Usman Hassan, Mudassar Hussain","doi":"10.1002/dc.70020","DOIUrl":"https://doi.org/10.1002/dc.70020","url":null,"abstract":"Introduction: Positive cerebrospinal fluid (CSF) cytology remains a cornerstone and irreplaceable diagnostic modality for the indication of leptomeningeal dissemination by primary as well as metastatic malignancies. Identification of tumor cells is fundamental for institution of aggressive treatment, such as radiotherapy, intrathecal chemotherapy, or systemic therapies, and will help increase overall survival. We did a retrospective analysis of CSF cytology in our center of all samples diagnosed as positive for malignant cells from January 2001 to December 2024 to find out about the spectrum of malignancies being detected in CSF cytology.Methods: We searched the database of our Hospital from January 2001 to December 2024 and found 853 samples of CSF of different patients that were diagnosed as positive for malignant cells. All cases were reviewed independently by two cytopathologists, and no discrepancy was found.Results: The diagnostic spectrum included cases of metastatic solid and hematolymphoid malignancies as well as dissemination of primary CNS tumors to CSF. Out of 853 cases, 550 cases were of CSF involvement by blast cells, 132 were of metastatic carcinomas, 78 were of hematological malignancies other than lymphoblastic lymphomas, 82 were of primary CNS tumors involving CSF, and 11 were of various other malignancies, including a spectrum of round blue cell tumors and sarcomas.Conclusions: A broad spectrum of epithelial, mesenchymal, and hematolymphoid malignancies can be diagnosed on CSF cytology, and these are likely metastatic in origin. Hematolymphoid malignancies, especially lymphoblastic lymphoma in children, are most common. Likewise, metastatic carcinomas constitute the major bulk of the adult population.","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Cytomegalovirus in EGFR-Mutated and Wild-Type Lung Adenocarcinoma: Detection in Cytological Smears and Associated Peripheral Immune Response. 人巨细胞病毒在egfr突变和野生型肺腺癌中的作用：细胞学涂片检测和相关的外周免疫反应

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-18 DOI: 10.1002/dc.70024

Suzana Harabajsa, Paula Gršković, Ivana Grgić, Lana Gorenec, Snježana Židovec Lepej, Sonja Badovinac, Marko Jakopović, Silvana Smojver-Ježek, Petra Korać

{"title":"Human Cytomegalovirus in EGFR-Mutated and Wild-Type Lung Adenocarcinoma: Detection in Cytological Smears and Associated Peripheral Immune Response.","authors":"Suzana Harabajsa, Paula Gršković, Ivana Grgić, Lana Gorenec, Snježana Židovec Lepej, Sonja Badovinac, Marko Jakopović, Silvana Smojver-Ježek, Petra Korać","doi":"10.1002/dc.70024","DOIUrl":"https://doi.org/10.1002/dc.70024","url":null,"abstract":"Background: Human cytomegalovirus (HCMV) has been proposed as a potential risk factor in lung cancer. This study aimed to investigate the relationship between the presence of HCMV in tumor cells and the immune response to HCMV in the peripheral blood of patients with EGFR-mutated and EGFR wild-type lung adenocarcinoma.Methods: DNA was extracted from 102 May-Grünwald-Giemsa-stained cytological smears (51 EGFR-mutated and 51 EGFR wild-type). HCMV presence in tumor cells was assessed by PCR targeting viral genes MIE and gB. The immune response to HCMV was evaluated in 80 corresponding peripheral blood samples using the QuantiFERON-CMV ELISA test. Statistical analysis was performed using the chi-square test, with significance p < 0.05.Results: HCMV MIE gene was detected in eight (15.7%) EGFR-mutated and one (2.0%) EGFR wild-type smear. The HCMV gB gene was identified in four (7.8%) EGFR wild-type smears and none of the EGFR-mutated smears. Immune response to HCMV was observed in 18 (62.1%) EGFR-mutated and 26 (50.9%) EGFR wild-type patients. Among those with detectable HCMV MIE, an immune response was found in two EGFR-mutated and one EGFR-negative patient. Among smears positive for HCMV gB, immune response was detected in three EGFR wild-type cases. One EGFR wild-type case showed no immune response despite the presence of HCMV gB.Conclusion: No clear association was observed between HCMV in lung adenocarcinoma cells and the immune response to HCMV in peripheral blood, regardless of EGFR gene mutation status.","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytopathology in the Philippines: A Chronicle of Practice, Purpose, and Progress. 菲律宾的细胞病理学：实践、目的和进步的编年史。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-17 DOI: 10.1002/dc.70023

Zubair Baloch

引用次数: 0

In-Depth Assessment of Cytologic Features in 106 Cervical Papanicolaou Tests of Transgender Male Patients on Testosterone: An Institutional Experience. 深入评估跨性别男性患者106例宫颈巴氏染色试验中睾丸激素的细胞学特征：一个机构经验。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-15 DOI: 10.1002/dc.70018

Juanita E Ferreira, Nikki Chiang, Xin Jing, Brian Smola, Richard L Cantley, Judy C Pang, Madelyn Lew

{"title":"In-Depth Assessment of Cytologic Features in 106 Cervical Papanicolaou Tests of Transgender Male Patients on Testosterone: An Institutional Experience.","authors":"Juanita E Ferreira, Nikki Chiang, Xin Jing, Brian Smola, Richard L Cantley, Judy C Pang, Madelyn Lew","doi":"10.1002/dc.70018","DOIUrl":"https://doi.org/10.1002/dc.70018","url":null,"abstract":"Background: There is a decreased cervical cancer screening rate among female to male transgender (FTMTG) patients. Data on the distinct cytologic features present in cervical cytology (CC) of those on testosterone therapy is limited.Methods: An 2017-2023 electronic database search identified CC specimens from a cohort of FTMTG patients on testosterone therapy (TT). A morphologic retrospective review of CC for cellularity and presence of key morphologic features was performed. Records were reviewed for original cytologic diagnoses and concurrent HPV test results for comparison with a cisgender female (CF) cohort.Results: 106 of 132,363 (0.08%) identified CC specimens were from FTMTG patients on TT. Diagnostic rates were compared to the CF population. The most common diagnosis for both groups was \"negative for intraepithelial lesion or malignancy\". The unsatisfactory rate was significantly higher in the FTMTG cohort at 21.7% (vs. 2.7%). The comparative HPV positivity rate of FTMTG and CF cohorts was 13.2% and 10.7%, respectively. Of 83 FTMTG satisfactory CC specimens, 67% showed low cellularity (narrowly meeting the adequacy threshold of 5000 well-visualized squamous cells) and 78% showed extensive squamous atrophy. Nuclear grooves and irregular contours (features associated with transitional cell metaplasia) were observed in 18% and 23%, respectively. High N:C ratio was noted in 20% of cases.Conclusion: The higher unsatisfactory rate in FTMTG patients raises the question of whether adequacy criteria for this cohort should be adjusted. To enhance diagnostic accuracy, providing an accurate clinical history may prevent overinterpretation of features associated with transitional cell metaplasia.","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metastatic Head and Neck Squamous Cell Carcinoma: Cytomorphological Spectrum on Fine Needle Aspiration Biopsy Cytology and Its Relevance. 转移性头颈部鳞状细胞癌：细针穿刺活检细胞学的细胞形态学谱及其相关性。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-13 DOI: 10.1002/dc.70019

Dipanwita Biswas, Aadya Kerkar, Nuli Muni Kiran, Sushmita Ghoshal, Suvradeep Mitra, Nalini Gupta, Amanjit Bal, Radhika Srinivasan

{"title":"Metastatic Head and Neck Squamous Cell Carcinoma: Cytomorphological Spectrum on Fine Needle Aspiration Biopsy Cytology and Its Relevance.","authors":"Dipanwita Biswas, Aadya Kerkar, Nuli Muni Kiran, Sushmita Ghoshal, Suvradeep Mitra, Nalini Gupta, Amanjit Bal, Radhika Srinivasan","doi":"10.1002/dc.70019","DOIUrl":"https://doi.org/10.1002/dc.70019","url":null,"abstract":"Background: Head and neck squamous cell carcinoma (HNSCC) frequently metastasizes to the cervical lymph nodes and is subjected to fine needle aspiration biopsy cytology (FNABC). The aim of this study was to describe the cytomorphological spectrum and explore the association with the primary site of origin and the short-term outcome following radiation therapy.Methods: A retrospective cytomorphological evaluation was performed on 338 cases of metastatic squamous cell carcinoma to cervical lymph nodes from HNSCC primaries. The clinical details including the age, sex, primary site of origin confirmed on histopathology, composite stage, and outcome at 1-year follow-up (86) were obtained from the records. An exploratory analysis was performed to derive any association of the cytomorphological features with the primary site of origin and short-term outcome.Results: The patients' age ranged from 28 to 89 years (mean, 56.6 years) with marked male preponderance, and the majority in Stage IV. The primary site was the oral cavity (45.2%) followed by the oropharynx (37%), hypopharynx (8.5%), nasopharynx (6.2%) and larynx (3.1%). Cytomorphology revealed keratinization of any degree in 86% of cases. The background was necroinflammatory, fluidy, or reactive lymphocytic with granulomatous reaction in 5% of cases. The chromatin pattern (vesicular, coarse or dense hyperchromatic), presence of nucleoli, and reactive lymphocytic background showed site-specific differences. However, no cytomorphological parameter showed any association with short-term outcome.Conclusion: Metastatic HNSCC to the cervical lymph nodes presents a broad cytomorphological spectrum with no specific association with outcome.","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145052420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid Biopsy in CRC Management: Early Detection, Minimal Residual Disease, and Therapy Optimization—Clinical Evidence and Challenges 液体活检在结直肠癌治疗中的应用：早期发现、最小残留疾病和优化治疗——临床证据和挑战。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-04 DOI: 10.1002/dc.70009

Qi Liu, Xiaoyong Li, Tiejun Jin, Sibo Huo, Shuai Su, Nan Liu

{"title":"Liquid Biopsy in CRC Management: Early Detection, Minimal Residual Disease, and Therapy Optimization—Clinical Evidence and Challenges","authors":"Qi Liu, Xiaoyong Li, Tiejun Jin, Sibo Huo, Shuai Su, Nan Liu","doi":"10.1002/dc.70009","DOIUrl":"10.1002/dc.70009","url":null,"abstract":"<div>\u0000 \u0000 Colorectal cancer (CRC) is a major global health burden, ranking among the leading causes of cancer-related deaths. Despite improvements in screening and treatment, challenges such as late-stage diagnosis, high recurrence rates, and therapy resistance continue to impede optimal outcomes. Liquid biopsy, a minimally invasive technique that analyzes tumor-derived components in bodily fluids—including circulating tumor DNA (ctDNA), circulating tumor cells (CTCs), and extracellular vesicles (EVs)—is emerging as a powerful tool to transform CRC management across the disease continuum. This review provides a comprehensive overview of liquid biopsy's current and emerging applications in CRC. We examine its role in early detection, where sensitive ctDNA-based assays and epigenetic biomarkers have demonstrated the ability to identify CRC at asymptomatic or early stages, potentially improving screening uptake and compliance. Furthermore, we explore how liquid biopsy enables dynamic monitoring of treatment response and clonal evolution, facilitating the timely identification of resistance mutations and supporting personalized therapy adjustments. Innovations in multi-omics integration, artificial intelligence, and ultra-sensitive sequencing technologies are also discussed as pivotal advancements that enhance the clinical utility of liquid biopsy. Despite significant progress, the widespread adoption of liquid biopsy faces several hurdles, including assay standardization, sensitivity for low-shedding tumors, regulatory approval, and cost-effectiveness. Continued research, validation in large prospective trials, and harmonization of testing protocols are essential to overcome these challenges. Ultimately, liquid biopsy holds the potential to become a cornerstone of precision oncology in CRC, enabling earlier intervention, more tailored treatment strategies, and improved patient outcomes.\u0000 </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 11","pages":"580-591"},"PeriodicalIF":1.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case Report: Cytopathological Diagnosis of Extracranial Anaplastic Meningioma Using Fine-Needle Aspiration. 病例报告：细针穿刺诊断颅外间变性脑膜瘤。

IF 1 4区医学

Diagnostic Cytopathology Pub Date : 2025-09-02 DOI: 10.1002/dc.70017

Burcu Özcan

引用次数: 0