Diagnostic Cytopathology最新文献

{"title":"Myelomatous Pleural Effusion: A Case Report.","authors":"Kinjal Patel, Natasha Modi, Avinash Munshi, Sima Chauhan, Pranab Dey","doi":"10.1002/dc.70101","DOIUrl":"10.1002/dc.70101","url":null,"abstract":"<p><p>Multiple myeloma is a relatively common hematologic malignancy; however, serous cavity effusions occur infrequently, and bona fide myelomatous effusions are exceedingly rare, typically representing an advanced, biologically aggressive variant associated with dismal outcomes. We report a compelling case of a 50-year-old patient with established multiple myeloma who developed leukemic transformation with concurrent pleural cavity infiltration while receiving systemic chemotherapy. The patient exhibited rapidly progressive dyspnea, a nonproductive cough, and debilitating fatigue in the days preceding admission. Cross-sectional imaging demonstrated a massive pleural effusion accompanied by partial ipsilateral pulmonary collapse. Cytopathologic examination of the pleural fluid revealed a predominance of atypical plasma cells and plasmablasts with marked dysmorphism, unequivocally confirming a myelomatous pleural effusion. Although hematologic neoplasms are well-recognized etiologies of body cavity effusions, the possibility of myelomatous involvement-albeit rare-must be judiciously entertained, as it heralds a distinct biologic trajectory and necessitates tailored therapeutic strategies that diverge substantially from the management of more common etiologies. This case underscores the need for heightened clinical vigilance and timely cytologic assessment in patients with multiple myeloma who present with new or unexplained serous effusions, as early identification of pleural involvement may influence both therapeutic decision-making and prognostication.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"E149-E152"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146257740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disseminated Histoplasmosis: An Unusual Finding at ROSE.","authors":"Erika E Doxtader, Jona Banzon, Sanjay Mukhopadhyay","doi":"10.1002/dc.70103","DOIUrl":"10.1002/dc.70103","url":null,"abstract":"<p><p>We describe an unusual case in which disseminated histoplasmosis was suspected at the time of of rapid on-site assessment (ROSE) performed during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). The patient was a renal transplant recipient with fever, mild anemia, bilateral lung infiltrates and mediastinal lymphadenopathy who underwent EBUS-TBNA of station 11L and 7 lymph nodes. The diagnosis of disseminated histoplasmosis was suggested at the time of ROSE and confirmed several days later by microbiologic cultures. Cytomorphologic findings on routine stains can be a powerful clue to the disseminated form of histoplasmosis.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"E146-E148"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13103701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Clinical Characteristics of 25 Patients With Pulmonary Nocardiosis Diagnosed by Next-Generation Sequencing.","authors":"Xuebo Duan, Qiangqiang Wang, Yingjie Ren","doi":"10.1002/dc.70104","DOIUrl":"10.1002/dc.70104","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the clinical characteristics of patients with pulmonary nocardiosis, enhance clinicians' understanding of the disease, and explore the clinical value of Next-Generation Sequencing (NGS) in diagnosing pulmonary nocardiosis.</p><p><strong>Methods: </strong>A retrospective analysis of clinical data from patients diagnosed with pulmonary nocardiosis by bronchoscopic alveolar lavage fluid NGS in our hospital from April 2022 to April 2024 was conducted. The clinical characteristics were analyzed, and their treatment and outcomes were tracked.</p><p><strong>Results: </strong>(1) Among the 25 patients with pulmonary nocardiosis, 10 were male, and 15 were female, aged 38-91 years, with an average age of 64.60 ± 12.40 and a BMI of 21.6 ± 3.06 kg/m². Six patients had a long-term smoking history, and two had smoked in the past. Six were farmers. Twenty-one patients had a history of underlying diseases, mainly chronic respiratory diseases such as bronchiectasis and COPD. Twenty patients had comorbidities, mainly respiratory failure, hypoxemia, liver and kidney dysfunction, and electrolyte disturbances. (2) The main clinical manifestations were fever, cough, sputum production, shortness of breath, fatigue, and poor appetite. Laboratory tests mainly showed elevated neutrophil percentage, hypersensitive C-reactive protein, erythrocyte sedimentation rate, procalcitonin, and decreased serum albumin. (3) Imaging of the chest revealed bilateral lung involvement in 16 patients, right lung involvement in 8, and left lung involvement in 1. The main imaging features included cavities, consolidation, exudation, and nodules. Bronchoscopy is an important way to obtain BALF. Bronchoscopic findings are nonspecific. They can be bronchial inflammatory changes, viscous secretions in the lumen, occlusion of the lumen, stenosis of the segment, etc. (4) Fourteen cases were identified as Nocardia cyriacigeorgica , six as Nocardia abscessus , three as Nocardia otitidiscaviarum , and two as Nocardia asteroides . Twenty-three patients received treatment; six were treated with sulfamethoxazole and trimethoprim (SMX/TMP) alone, 17 with a combination of sulfonamide and linezolid or other drugs, one was discharged automatically, and the remaining 22 showed improvement.</p><p><strong>Conclusion: </strong>Pulmonary nocardiosis often occurs in patients with chronic respiratory diseases and has nonspecific clinical features, making misdiagnosis easy. NGS can rapidly and accurately diagnose this disease in patient specimens. Early, adequate, and long-term combined therapy based on sulfonamide generally leads to a good prognosis.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"417-428"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147270147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classifying Liver Cytology Using the WHO Reporting System: Institutional Experience and Malignancy Risk Assessment.","authors":"Saroja Devi Geetha, Amr Ali, Meena Kashi, Alexander Burke, Philip Ort, Alanna Chiu, Myungji Chun, Karen Chau, Kasturi Das, Deepika Savant","doi":"10.1002/dc.70112","DOIUrl":"10.1002/dc.70112","url":null,"abstract":"<p><strong>Aim: </strong>The World Health Organization's (WHO) Reporting System for Liver Cytopathology aims to standardize communication with clinicians globally to improve patient management. Our study reclassifies liver cytology reports based on the WHO classification and assesses the risk of malignancy (ROM) in each category.</p><p><strong>Methods: </strong>Liver cytology reports from 2019 to 2023 were retrieved to obtain demographic details, clinical data, cytology, and surgical follow-up (SFU). Cytology diagnoses were reclassified using the WHO classification. ROM was analyzed based on SFU.</p><p><strong>Results: </strong>Our study had 976 cases, age ranged from 1-96 years, with an average age of 67 years. Male: female ratio was 1:1. Majority of lesions (400/525, 76%) were less than 5 cm. Location of the lesion was available in 595 cases, with right lobe being the most common (64%; n = 383), followed by left lobe (36%, n = 212). 883 cases (90%) underwent rapid onsite evaluation. Reclassification using the WHO classification system into 5 categories included non-diagnostic (n = 79), benign (n = 117), atypical (n = 25), suspicious for malignancy (n = 6) and malignant (n = 749). Thirty-one cases (3%) had cytology follow up, 71(7.3%) had SFU. The ROM in the SFU cohort was 17% (1/6; 95% CI: 10%-76%) in non-diagnostic, 23% (3/13, 95% CI: 15.4%-30.6%) in benign and 100% in atypical (3/3, 95% CI: 88%-100%), suspicious for malignancy (2/2, 95% CI: 50%-100%) and malignant(47/47, 95% CI: 99.5%-100%) categories. Repeat FNA yielded malignant upgrade rates of 80% (4 out of 5) in non-diagnostic and 75% (3 out of 4) in atypical categories.</p><p><strong>Conclusion: </strong>We present the values of ROM in the various categories of the WHO Reporting System for Liver Cytopathology using a large cohort of cases. Repeat sampling is recommended in non-diagnostic and atypical categories based on an increase in malignant upgrades in these categories.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"436-443"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147431334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytologic Findings in Esophageal Perforation: An Institutional Experience With Pleural Fluid Specimens.","authors":"Mason Marshall, Samer Khader, Sigfred Lajara, Liron Pantanowitz","doi":"10.1002/dc.70111","DOIUrl":"10.1002/dc.70111","url":null,"abstract":"<p><strong>Introduction: </strong>Esophageal perforation (EP) is a rare but life-threatening condition, and most cases are due to iatrogenic causes. The rest occur spontaneously, due to malignancies, or trauma. The mortality rates can approach 50%, and delayed diagnosis of EP exacerbates patient outcomes. Cytologic recognition of pleural fluid specimens with oropharyngeal contents (e.g., squamous cells, Candida species) or food/foreign material can expedite the diagnosis of EP. This study presents our institution's experience with cytologic findings in EP patients.</p><p><strong>Materials and methods: </strong>We reviewed our institutional database over a 25-year period to identify pleural fluid cytology specimens from EP patients. Data on patient demographics, clinical history, and microbiology culture results were collected. Slides were assessed for squamous cells, food/foreign material, and infectious organisms.</p><p><strong>Results: </strong>Eleven pleural fluid cytology specimens from 10 patients with EP (seven males, three females; mean age 68 years) were retrieved. Three cases were atypical, and eight were negative for malignancy. Mortality occurred in four (40%) patients within a mean of 2.2 months post-EP diagnosis. The majority of EP causes were iatrogenic (82%) or associated with malignancy (55%). Squamous cells were present in 27% of specimens, while food/foreign material and fungi were identified in 36% and 9% of cases, respectively. Cultures were positive in seven (64%) patients, with 46% showing bacterial growth and 55% positive for fungal organisms.</p><p><strong>Conclusions: </strong>Our experience corroborates that EP carries a high mortality risk, underscoring the need for prompt diagnosis. Although Candida species were present in only a small subset of pleural fluid samples, the presence of squamous cells or food/foreign material is a helpful diagnostic clue of EP, particularly in patients with a history of recent medical manipulation or malignancy involving the esophagus. Our findings highlight the potential of identifying involved pleural fluid cytology specimens to decrease the time to diagnosis of EP.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"429-435"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13103686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Atypical and Suspicious for Malignancy Categories of the WHO Reporting System for Lymph Node, Spleen, and Thymus Cytopathology: Review of their Diagnostic Utility, Limitations, and Clinical Impact.","authors":"Immacolata Cozzolino, Mats Ehinger, Oscar Lin, Elisabetta Maffei, Pio Zeppa, Andrew S Field","doi":"10.1002/dc.70118","DOIUrl":"10.1002/dc.70118","url":null,"abstract":"<p><strong>Background: </strong>Fine-needle aspiration biopsy (FNAB) is the preferred first-line diagnostic tool for evaluating lymphadenopathy due to its minimally invasive nature and cost-effectiveness. However, cytopathological interpretation of lymph node FNAB remains challenging because of the wide morphological spectrum of lymphoid lesions. The World Health Organization (WHO) Reporting System for Lymph Nodes, Spleen, and Thymus Cytopathology (WHO System) incorporates the \"Atypical\" and \"Suspicious for malignancy\" (\"SFM\") categories to manage diagnostic uncertainty, yet their reproducibility and clinical implications remain undetermined.</p><p><strong>Methods: </strong>This review with illustrative case-based applications of the WHO System discusses the use, diagnostic performance, and limitations of the \"Atypical\" and \"SFM\" categories. Comparative analysis of published data, including interobserver concordance and risk of malignancy (ROM) studies, has been performed, with reference to the Sydney System and the multicenter DELYCYOUS study.</p><p><strong>Results: </strong>Cases categorized as \"Atypical\" demonstrate highly variable ROM (28.6%-76.9%, mean ≈65%), reflecting heterogeneity in application and ancillary test integration, whereas the \"SFM\" category consistently exhibits high ROM (82%-100%, mean ≈95%). Interobserver agreement remains poor (κ = 0.075 for \"Atypical\"; κ = 0.104 for \"Suspicious for malignancy\"), highlighting interpretive subjectivity. Diagnostic pitfalls include artifactual monomorphism mimicking high-grade lymphoma and underdiagnosis of paucicellular Hodgkin lymphoma or T-cell proliferations. Ancillary tests, while essential, may also yield misleading results-such as monoclonal B-cell populations in reactive conditions-potentially leading to overinterpretation.</p><p><strong>Conclusions: </strong>The \"Atypical\" and \"SFM\" categories are indispensable for diagnostic stratification and clinical management but suffer from limited reproducibility and intrinsic ambiguity. Their optimal application requires standardized diagnostic criteria of specific lesions, which are provided in the WHO System, institutional consistency, and integrated use of cytomorphological, immunophenotypical, and molecular data to minimize diagnostic uncertainty and improve patient outcomes.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"449-460"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13103705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147653928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pancreatic Adenocarcinoma: Diagnosis by Ultrasound-Guided Fine-Needle Aspiration-Cyto-Histological Correlation and Experience at a Multidisciplinary Tertiary Reference Center.","authors":"Judith González-López, Mónica Elizabeth Bauza, Judith Pérez-Rojas, Cecilia López-Valdivia, Carlos Zac Romero, Óscar Castells-Perona, Cristina Ballester-Ibáñez, Eva Montalvá-Orón, Rafael López-Andújar, Lidia Argüello-Viudez, Mireya Prieto Rodríguez","doi":"10.1002/dc.70097","DOIUrl":"10.1002/dc.70097","url":null,"abstract":"<p><strong>Background: </strong>Pancreatic adenocarcinoma (PDAC) is characterized by its poor prognosis. Cytology is essential for diagnostic confirmation and ancillary studies. The endoscopic ultrasound (EUS) set a precedent, facilitating cytological sampling through fine-needle aspiration (FNA), with a sensitivity of 93% and a specificity of 95%. In this work, we analyzed all EUS-guided pancreatic FNAs performed at our center over 2014-2025 and compared the cytological findings with the corresponding surgical specimen results.</p><p><strong>Methods: </strong>Observational, descriptive, and retrospective study of all EUS-guided pancreatic FNAs performed at our center (2014-January 2025) in patients ≥ 18 years and their corresponding surgical specimens. Samples were obtained using a linear echoendoscope and 25G needles, processed with Romanowsky and Papanicolaou stains, and cell blocks prepared for immunohistochemistry. Cytological diagnoses followed WHO criteria; histology from surgical specimens served as the reference standard. Statistical analysis was performed using SPSS v.22 with p < 0.05 considered significant.</p><p><strong>Results: </strong>One hundred and sixty-three EUS-guided pancreatic FNAs were performed and 34 patients underwent surgical treatment (20.8%), 31 of them (91.2%) with a cytological diagnosis of malignancy. Thirty-three patients had malignant lesions (97%) in the surgical specimen. Cytology showed a sensitivity of 96.9%, a specificity of 100%, a positive predictive value of 100%, and a negative predictive value of 50%. Cyto-histological correlation was 91.2% with a κ = 0.654 (p < 0.001). Cytopathologists' expertise was statistically significant (p < 0.001).</p><p><strong>Conclusions: </strong>Cytology is highly reliable for confirming malignancy. Final management will be multidisciplinary.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"403-416"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13103707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid-Based Cytologic Features of a Thyroid TFE3-Rearranged PEComa-Like Neoplasm: A Case Report.","authors":"Wen-Ying Lee, Sheng-Tsung Chang, Lee-E Huang","doi":"10.1002/dc.70108","DOIUrl":"10.1002/dc.70108","url":null,"abstract":"<p><p>Perivascular epithelioid cell tumor (PEComa) is a rare mesenchymal neoplasm, and primary involvement of the thyroid gland is exceedingly rare. TFE3-rearranged PEComas constitute a distinct molecular subset characterized by epithelioid morphology and strong nuclear TFE3 expression. Cytologic descriptions of PEComa are limited, and to date, only three cytologic cases of TFE3-rearranged PEComa or PEComa-like neoplasm have been reported in the literature, none of which involved the thyroid gland. We report a case of thyroid TFE3-rearranged PEComa-like neoplasm in a 25-year-old woman who presented with a palpable thyroid nodule. Fine-needle aspiration using liquid-based cytology revealed a hypercellular specimen composed predominantly of dispersed and loosely cohesive epithelioid cells with enlarged round to oval nuclei, fine chromatin, and frequent intranuclear pseudoinclusions, resulting in an initial interpretation suspicious for papillary thyroid carcinoma. Histologic examination demonstrated a well-circumscribed epithelioid neoplasm with pseudoalveolar architecture supported by a delicate arborizing vascular network. Immunohistochemical studies showed tumor cell positivity for TFE3, cathepsin K, desmin (patchy), and vimentin, with negativity for epithelial, thyroid-specific, melanocytic, and other smooth muscle markers, as well as PAX8, CD10, MiTF, and PAS/PAS-D stains, arguing against metastatic renal cell carcinoma and alveolar soft part sarcoma. The patient underwent surgical resection and remains disease-free after 7.5 years of follow-up. This case represents the first description of the liquid-based cytologic features of a thyroid TFE3-rearranged PEComa-like neoplasm. Awareness of this rare entity and its cytologic overlap with papillary thyroid carcinoma is essential to avoid diagnostic pitfalls in thyroid fine-needle aspiration.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"E160-E164"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unsatisfactory ThinPrep Pap Tests: An Educational Approach to Quality.","authors":"Kelly Olson, Erek Kucher, Ashley Rasmussen, Suzanne M Selvaggi","doi":"10.1002/dc.70115","DOIUrl":"10.1002/dc.70115","url":null,"abstract":"<p><strong>Background: </strong>The unsatisfactory percentage for ThinPrep Pap Tests (TPPT) at our institution reached the 75th percentile (2.7%) benchmark established by the College of American Pathologists (CAP). Our aim was to identify outliers by analysis of data, provide education and feedback, and establish a system to monitor outcomes.</p><p><strong>Methods: </strong>The laboratory information system (LIS) was queried for TPPT cases diagnosed as 'Non-diagnostic' between April and June 2022. Two hundred and thirty-two cases were identified and reviewed by 2 cytopathologists and 1 cytotechnologist to identify limiting factors, including cellularity, blood, lubricant, inflammation, and mucus. The percentage of unsatisfactory TPPT was evaluated for both clinicians and clinics and stratified into peer groups based on total TPPT collected. The two clinics with the highest unsatisfactory percentage in a peer group of > 500 TPPT collected were identified. A LIS report was created to improve efficiency in future data collection.</p><p><strong>Results: </strong>Low cellularity was a limiting factor in 100% of cases, blood in 51.7% (n = 120), lubricant in 18.5% (n = 43), inflammation in 13.8% (n = 32), and mucus in 3.9% (n = 9), with multiple factors possible per case. Twenty clinics collected > 500 TPPT for FY2022. Two clinics (1 resident-led clinic) had an unsatisfactory percentage > 3%, for which to target interventions. Clinic 1 had an unsatisfactory percentage of 6.1% (62/1018), and clinic 2 was 3.5% (21/608).</p><p><strong>Conclusions: </strong>To address limiting factors, a laboratory bulletin with best practices for TPPT collection and a list of Hologic TPPT approved lubricants was sent to each clinic. Statistics of unsatisfactory TPPT were provided. The highest unsatisfactory TPPT clinics received Hologic educational materials and an educational session. The resolution of laboratory quality issues requires investigations, often laborious in nature. In an era of staffing shortages, an effective LIS is essential to efficiently investigate and resolve quality variances. TPPT data via LIS reports were reviewed at 3 months and 1-year post-intervention implementation to evaluate outcomes.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"444-448"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary Adrenal BCOR::CCNB3 Sarcoma: Fine Needle Aspiration Cytology Findings.","authors":"Simran Meet, Aanchal Kakkar, Shilpa Sharma, Manisha Jana, Venkateswaran K Iyer","doi":"10.1002/dc.70107","DOIUrl":"10.1002/dc.70107","url":null,"abstract":"<p><p>Pediatric adrenal tumors are uncommon, with the most common being peripheral neuroblastic tumors. Other neoplasms like adrenal cortical tumors, pheochromocytoma, and myelolipoma may also be encountered in this age group. Rare instances of mesenchymal tumors such as Ewing sarcoma and rhabdomyosarcoma have been described. We report the fine needle aspiration cytology (FNAC) findings of the first case of BCOR-altered sarcoma occurring in the adrenal gland. A 13-year-old boy presented with severe abdominal pain. Imaging revealed a mass in the right adrenal gland. FNAC showed loosely cohesive clusters and singly dispersed medium-sized polygonal to spindled tumor cells with scant to moderate delicate cytoplasm and normochromic, mildly pleomorphic nuclei with fine granular chromatin. Slender fibrovascular cores traversed tumor fragments. Histopathology of the adrenalectomy showed sheets of round to spindled tumor cells interrupted by thin fibrovascular septae, with foci of necrosis. BCOR, SATB2, and cyclin D1 were positive, while CD99, NKX2.2, WT1, desmin, and myogenin were negative. Fluorescence in situ hybridization revealed BCOR::CCNB3 fusion, consistent with BCOR-rearranged sarcoma. BCOR-rearranged sarcomas are rare malignant neoplasms. As primary treatment is chemotherapy, accurate diagnosis on limited material such as FNAC is critical. The recognition of cytological features and follow up with appropriate ancillary testing has the potential to improve management of these patients.</p>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":" ","pages":"E153-E159"},"PeriodicalIF":1.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}