Current topics in membranes最新文献_第8页

4区生物学

Current topics in membranes Pub Date : 2022-01-01 Epub Date: 2022-08-01 DOI: 10.1016/bs.ctm.2022.06.004

Vivian C Onyali, Timothy L Domeier

引用次数: 1

Specificity of Ca²⁺-activated K⁺ channel modulation in atherosclerosis and aerobic exercise training. Ca2+激活的K+通道调节在动脉粥样硬化和有氧运动训练中的特异性。

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/bs.ctm.2022.09.005

Eric A Mokelke, Mouhamad Alloosh, Michael Sturek

{"title":"Specificity of Ca2+-activated K+ channel modulation in atherosclerosis and aerobic exercise training.","authors":"Eric A Mokelke, Mouhamad Alloosh, Michael Sturek","doi":"10.1016/bs.ctm.2022.09.005","DOIUrl":"https://doi.org/10.1016/bs.ctm.2022.09.005","url":null,"abstract":"Vascular smooth muscle cells express several isoforms of a number of classes of K+ channels. Potassium channels play critical roles in the regulation of vascular smooth muscle contraction as well as vascular smooth muscle cell proliferation or phenotypic modulation. There is ample evidence that it is Ca2+ that enables these two seemingly disparate functions to be tightly coupled both in healthy and disease processes. Because of the central position that potassium channels have in vasocontraction, vasorelaxation, membrane potential, and smooth muscle cell proliferation, these channels continue to possess the potential to serve as novel therapeutic targets in cardiovascular disease. While there are questions that remain regarding the complete interactions between K+ channels, vascular regulation, smooth muscle cell proliferation, and phenotypic modulation in physiological and pathophysiological conditions, a broad understanding of the contributions of each class of K+ channel to contractile and proliferative states of the vasculature has been reached. This brief review will discuss the current understanding of the role of K+ channels in vascular smooth muscle cells in health and disease using the porcine vascular smooth muscle cell model with particular attention to new scientific discoveries contributed by the authors regarding the effect of endurance exercise on the function of the K+ channels.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":"90 ","pages":"123-139"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10627169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiphasic changes in smooth muscle Ca²⁺ transporters during the progression of coronary atherosclerosis. 冠状动脉粥样硬化过程中平滑肌Ca2+转运蛋白的多相变化。

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/bs.ctm.2022.09.007

Jill Badin, Stacey Rodenbeck, Mikaela L McKenney-Drake, Michael Sturek

{"title":"Multiphasic changes in smooth muscle Ca2+ transporters during the progression of coronary atherosclerosis.","authors":"Jill Badin, Stacey Rodenbeck, Mikaela L McKenney-Drake, Michael Sturek","doi":"10.1016/bs.ctm.2022.09.007","DOIUrl":"https://doi.org/10.1016/bs.ctm.2022.09.007","url":null,"abstract":"Ischemic heart disease due to macrovascular atherosclerosis and microvascular dysfunction is the major cause of death worldwide and the unabated increase in metabolic syndrome is a major reason why this will continue. Intracellular free Ca2+ ([Ca2+]i) regulates a variety of cellular functions including contraction, proliferation, migration, and transcription. It follows that studies of vascular Ca2+ regulation in reductionist models and translational animal models are vital to understanding vascular health and disease. Swine with metabolic syndrome (MetS) develop the full range of coronary atherosclerosis from mild to severe disease. Intravascular imaging enables quantitative measurement of atherosclerosis in vivo, so viable coronary smooth muscle (CSM) cells can be dispersed from the arteries to enable Ca2+ transport studies in native cells. Transition of CSM from the contractile phenotype in the healthy swine to the proliferative phenotype in mild atherosclerosis was associated with increases in SERCA activity, sarcoplasmic reticulum Ca2+, and voltage-gated Ca2+ channel function. In vitro organ culture confirmed that SERCA activation induces CSM proliferation. Transition from the proliferative to a more osteogenic phenotype was associated with decreases in all three Ca2+ transporters. Overall, there was a biphasic change in Ca2+ transporters over the progression of atherosclerosis in the swine model and this was confirmed in CSM from failing explanted hearts of humans. A major determinant of endolysosome content in human CSM is the severity of atherosclerosis. In swine CSM endolysosome Ca2+ release occurred through the TPC2 channel. We propose a multiphasic change in Ca2+ transporters over the progression of coronary atherosclerosis.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":"90 ","pages":"95-121"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10627172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface. 序言

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/S1063-5823(22)00036-9

引用次数: 0

Role of TRPV4 Channels in Different Organ Systems TRPV4通道在不同器官系统中的作用

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/s1063-5823(22)x0002-1

引用次数: 0

Regulation of exosome release by lysosomal acid ceramidase in coronary arterial endothelial cells: Role of TRPML1 channel. 溶酶体酸性神经酰胺酶对冠状动脉内皮细胞外泌体释放的调节：TRPML1通道的作用。

4区生物学

Current topics in membranes Pub Date : 2022-01-01 Epub Date: 2022-10-03 DOI: 10.1016/bs.ctm.2022.09.002

Guangbi Li, Dandan Huang, Pengyang Li, Xinxu Yuan, Viktor Yarotskyy, Pin-Lan Li

{"title":"Regulation of exosome release by lysosomal acid ceramidase in coronary arterial endothelial cells: Role of TRPML1 channel.","authors":"Guangbi Li, Dandan Huang, Pengyang Li, Xinxu Yuan, Viktor Yarotskyy, Pin-Lan Li","doi":"10.1016/bs.ctm.2022.09.002","DOIUrl":"10.1016/bs.ctm.2022.09.002","url":null,"abstract":"Lysosomal acid ceramidase (AC) has been reported to determine multivesicular body (MVB) fate and exosome secretion in different mammalian cells including coronary arterial endothelial cells (CAECs). However, this AC-mediated regulation of exosome release from CAECs and associated underlying mechanism remain poorly understood. In the present study, we hypothesized that AC controls lysosomal Ca2+ release through TRPML1 channel to regulate exosome release in murine CAECs. To test this hypothesis, we isolated and cultured CAECs from WT/WT and endothelial cell-specific Asah1 gene (gene encoding AC) knockout mice. Using these CAECs, we first demonstrated a remarkable increase in exosome secretion and significant reduction of lysosome-MVB interaction in CAECs lacking Asah1 gene compared to those cells from WT/WT mice. ML-SA1, a TRPML1 channel agonist, was found to enhance lysosome trafficking and increase lysosome-MVB interaction in WT/WT CAECs, but not in CAECs lacking Asah1 gene. However, sphingosine, an AC-derived sphingolipid, was able to increase lysosome movement and lysosome-MVB interaction in CAECs lacking Asah1 gene, leading to reduced exosome release from these cells. Moreover, Asah1 gene deletion was shown to substantially inhibit lysosomal Ca2+ release through suppression of TRPML1 channel activity in CAECs. Sphingosine as an AC product rescued the function of TRPML1 channel in CAECs lacking Asah1 gene. These results suggest that Asah1 gene defect and associated deficiency of AC activity may inhibit TRPML1 channel activity, thereby reducing MVB degradation by lysosome and increasing exosome release from CAECs. This enhanced exosome release from CAECs may contribute to the development of coronary arterial disease under pathological conditions.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":"90 ","pages":"37-63"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842397/pdf/nihms-1861805.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10057071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endothelial TRPV4 channels in lung edema and injury. 内皮TRPV4通道在肺水肿和损伤中的作用。

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/bs.ctm.2022.07.001

Swapnil K Sonkusare, Victor E Laubach

引用次数: 5

Transient receptor potential vanilloid type 4 (TRPV4) in urinary bladder structure and function. 瞬时受体电位香草样蛋白4 (TRPV4)在膀胱结构和功能中的作用。

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/bs.ctm.2022.06.002

Megan Elizabeth Perkins, Margaret A Vizzard

{"title":"Transient receptor potential vanilloid type 4 (TRPV4) in urinary bladder structure and function.","authors":"Megan Elizabeth Perkins, Margaret A Vizzard","doi":"10.1016/bs.ctm.2022.06.002","DOIUrl":"https://doi.org/10.1016/bs.ctm.2022.06.002","url":null,"abstract":"Bladder pain syndrome (BPS)/interstitial cystitis (IC) is a urologic, chronic pelvic pain syndrome characterized by pelvic pain, pressure, or discomfort with urinary symptoms. Symptom exacerbation (flare) is common with multiple, perceived triggers including stress. Multiple transient receptor potential (TRP) channels (TRPA1, TRPV1, TRPV4) expressed in the bladder have specific tissue distributions in the lower urinary tract (LUT) and are implicated in bladder disorders including overactive bladder (OAB) and BPS/IC. TRPV4 channels are strong candidates for mechanosensors in the urinary bladder and TRPV4 antagonists are promising therapeutic agents for OAB. In this perspective piece, we address the current knowledge of TRPV4 distribution and function in the LUT and its plasticity with injury or disease with an emphasis on BPS/IC. We review our studies that extend the knowledge of TRPV4 in urinary bladder function by focusing on (i) TRPV4 involvement in voiding dysfunction, pelvic pain, and non-voiding bladder contractions in NGF-OE mice; (ii) distention-induced luminal ATP release mechanisms and (iii) involvement of TRPV4 and vesicular release mechanisms. Finally, we review our lamina propria studies in postnatal rat studies that demonstrate: (i) the predominance of the TRPV4+ and PDGFRα+ lamina propria cellular network in early postnatal rats; (ii) the ability of exogenous mediators (i.e., ATP, TRPV4 agonist) to activate and increase the number of lamina propria cells exhibiting active Ca2+ events; and (iii) the ability of ATP and TRPV4 agonist to increase the rate of integrated Ca2+ activity corresponding to coupled lamina propria network events and the formation of propagating wavefronts.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":"89 ","pages":"95-138"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486315/pdf/nihms-1929075.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10247788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An unexpected effect of risperidone reveals a nonlinear relationship between cytosolic Ca²⁺ and mitochondrial Ca²⁺ uptake. 利培酮的一个意想不到的影响揭示了细胞质Ca2+和线粒体Ca2+摄取之间的非线性关系。

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/bs.ctm.2022.09.001

Olaf A Bachkoenig, Benjamin Gottschalk, Roland Malli, Wolfgang F Graier

{"title":"An unexpected effect of risperidone reveals a nonlinear relationship between cytosolic Ca2+ and mitochondrial Ca2+ uptake.","authors":"Olaf A Bachkoenig, Benjamin Gottschalk, Roland Malli, Wolfgang F Graier","doi":"10.1016/bs.ctm.2022.09.001","DOIUrl":"https://doi.org/10.1016/bs.ctm.2022.09.001","url":null,"abstract":"Mitochondria actively contribute to cellular Ca2+ homeostasis. The molecular mechanisms of mitochondrial Ca2+ uptake and release are well characterized and are attributed to the multi-protein assembly of the mitochondrial Ca2+ uniporter complex (MCUC) and the mitochondrial sodium-calcium exchanger (NCLX), respectively. Hence, Ca2+ transfer from the endoplasmic reticulum (ER) and store-operated Ca2+ entry (SOCE) into the mitochondrial matrix has been quantitatively visualized on the subcellular level using targeted fluorescent biosensors. However, a correlation between the amplitude of cytosolic Ca2+ elevation with that in the mitochondrial matrix has not been investigated in detail so far. In the present study, we combined the Ca2+-mobilizing agonist histamine with the H1-receptor antagonist risperidone to establish a well-tunable experimental approach allowing the correlation between low, slow, high, and fast cytosolic and mitochondrial Ca2+ signals in response to inositol 1,4,5-trisphosphate (IP3)-triggered ER Ca2+ release. Our present data confirm a defined threshold in cytosolic Ca2+, which is necessary for the activation of mitochondrial Ca2+ uptake. Moreover, our data support the hypothesis of different modes of mitochondrial Ca2+ uptake depending on the source of the ion (i.e., ER vs SOCE).","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":"90 ","pages":"13-35"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10627170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vascular Ca_V1.2 channels in diabetes. 糖尿病血管CaV1.2通道。

4区生物学

Current topics in membranes Pub Date : 2022-01-01 DOI: 10.1016/bs.ctm.2022.09.003

Eric A Pereira da Silva, Miguel Martín-Aragón Baudel, Junyoung Hong, Peter Bartels, Manuel F Navedo, Madeline Nieves-Cintrón

{"title":"Vascular CaV1.2 channels in diabetes.","authors":"Eric A Pereira da Silva, Miguel Martín-Aragón Baudel, Junyoung Hong, Peter Bartels, Manuel F Navedo, Madeline Nieves-Cintrón","doi":"10.1016/bs.ctm.2022.09.003","DOIUrl":"https://doi.org/10.1016/bs.ctm.2022.09.003","url":null,"abstract":"Diabetic vasculopathy is a significant cause of morbidity and mortality in the diabetic population. Hyperglycemia, one of the central metabolic abnormalities in diabetes, has been associated with vascular dysfunction due to endothelial cell damage. However, studies also point toward vascular smooth muscle as a locus for hyperglycemia-induced vascular dysfunction. Emerging evidence implicates hyperglycemia-induced regulation of vascular L-type Ca2+ channels CaV1.2 as a potential mechanism for vascular dysfunction during diabetes. This chapter summarizes our current understanding of vascular CaV1.2 channels and their regulation during physiological and hyperglycemia/diabetes conditions. We will emphasize the role of CaV1.2 in vascular smooth muscle, the effects of elevated glucose on CaV1.2 function, and the mechanisms underlying its dysregulation in hyperglycemia and diabetes. We conclude by examining future directions and gaps in knowledge regarding CaV1.2 regulation in health and during diabetes.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":"90 ","pages":"65-93"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10627171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1