Current topics in membranes最新文献_第8页

SWI/SNF (BAF) complexes: From framework to a functional role in endothelial mechanotransduction. SWI/SNF (BAF)复合物:从框架到内皮机械转导的功能作用。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-10-09 DOI: 10.1016/bs.ctm.2021.09.006

Sandeep Kumar

{"title":"SWI/SNF (BAF) complexes: From framework to a functional role in endothelial mechanotransduction.","authors":"Sandeep Kumar","doi":"10.1016/bs.ctm.2021.09.006","DOIUrl":"https://doi.org/10.1016/bs.ctm.2021.09.006","url":null,"abstract":"Endothelial cells (ECs) are constantly subjected to an array of mechanical cues, especially shear stress, due to their luminal placement in the blood vessels. Blood flow can regulate various aspects of endothelial biology and pathophysiology by regulating the endothelial processes at the transcriptomic, proteomic, miRNomic, metabolomics, and epigenomic levels. ECs sense, respond, and adapt to altered blood flow patterns and shear profiles by specialized mechanisms of mechanosensing and mechanotransduction, resulting in qualitative and quantitative differences in their gene expression. Chromatin-regulatory proteins can regulate transcriptional activation by modifying the organization of nucleosomes at promoters, enhancers, silencers, insulators, and locus control regions. Recent research efforts have illustrated that SWI/SNF (SWItch/Sucrose Non-Fermentable) or BRG1/BRM-associated factor (BAF) complex regulates DNA accessibility and chromatin structure. Since the discovery, the gene-regulatory mechanisms of the BAF complex associated with chromatin remodeling have been intensively studied to investigate its role in diverse disease phenotypes. Thus far, it is evident that (1) the SWI/SNF complex broadly regulates the activity of transcriptional enhancers to control lineage-specific differentiation and (2) mutations in the BAF complex proteins lead to developmental disorders and cancers. It is unclear if blood flow can modulate the activity of SWI/SNF complex to regulate EC differentiation and reprogramming. This review emphasizes the integrative role of SWI/SNF complex from a structural and functional standpoint with a special reference to cardiovascular diseases (CVDs). The review also highlights how regulation of this complex by blood flow can lead to the discovery of new therapeutic interventions for the treatment of endothelial dysfunction in vascular diseases.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39560069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell. 荧光感应细胞的生物能量和物理化学状态。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-02 DOI: 10.1016/bs.ctm.2021.10.002

Luca Mantovanelli, Bauke F Gaastra, Bert Poolman

{"title":"Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell.","authors":"Luca Mantovanelli, Bauke F Gaastra, Bert Poolman","doi":"10.1016/bs.ctm.2021.10.002","DOIUrl":"https://doi.org/10.1016/bs.ctm.2021.10.002","url":null,"abstract":"Fluorescence-based sensors play a fundamental role in biological research. These sensors can be based on fluorescent proteins, fluorescent probes or they can be hybrid systems. The availability of a very large dataset of fluorescent molecules, both genetically encoded and synthetically produced, together with the structural insights on many sensing domains, allowed to rationally design a high variety of sensors, capable of monitoring both molecular and global changes in living cells or in in vitro systems. The advancements in the fluorescence-imaging field helped researchers to obtain a deeper understanding of how and where specific changes occur in a cell or in vitro by combining the readout of the fluorescent sensors with the spatial information provided by fluorescent microscopy techniques. In this review we give an overview of the state of the art in the field of fluorescent biosensors and fluorescence imaging techniques, and eventually guide the reader through the choice of the best combination of fluorescent tools and techniques to answer specific biological questions. We particularly focus on sensors for probing the bioenergetics and physicochemical status of the cell.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39690556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mechanical forces and metabolic changes cooperate to drive cellular memory and endothelial phenotypes. 机械力和新陈代谢变化共同驱动细胞记忆和内皮表型。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-09-25 DOI: 10.1016/bs.ctm.2021.07.003

Jin Li, Yun Fang, David Wu

{"title":"Mechanical forces and metabolic changes cooperate to drive cellular memory and endothelial phenotypes.","authors":"Jin Li, Yun Fang, David Wu","doi":"10.1016/bs.ctm.2021.07.003","DOIUrl":"10.1016/bs.ctm.2021.07.003","url":null,"abstract":"Endothelial cells line the innermost layer of arterial, venous, and lymphatic vascular tree and accordingly are subject to hemodynamic, stretch, and stiffness mechanical forces. Normally quiescent, endothelial cells have a hemodynamic set point and become \"activated\" in response to disturbed hemodynamics, which may signal impending nutrient or gas depletion. Endothelial cells in the majority of tissue beds are normally inactivated and maintain vessel barrier functions, are anti-inflammatory, anti-coagulant, and anti-thrombotic. However, under aberrant mechanical forces, endothelial signaling transforms in response, resulting cellular changes that herald pathological diseases. Endothelial cell metabolism is now recognized as the primary intermediate pathway that undergirds cellular transformation. In this review, we discuss the various mechanical forces endothelial cells sense in the large vessels, microvasculature, and lymphatics, and how changes in environmental mechanical forces result in changes in metabolism, which ultimately influence cell physiology, cellular memory, and ultimately disease initiation and progression.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639155/pdf/nihms-1758401.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39558573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular Mechanotransduction Mechanisms in Cardiovascular and Fibrotic Diseases 心血管和纤维化疾病的细胞机械转导机制

4区生物学

Current topics in membranes Pub Date : 2021-01-01 DOI: 10.1016/s1063-5823(21)x0002-6

引用次数: 1

Studying cell volume beyond cell volume. 研究细胞体积以外的细胞体积。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-10-02 DOI: 10.1016/bs.ctm.2021.08.001

Michael A Model

引用次数: 0

"Enhancing" mechanosensing: Enhancers and enhancer-derived long non-coding RNAs in endothelial response to flow. "增强 "机械传感：内皮细胞对血流反应中的增强子和增强子衍生的长非编码 RNA。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-09-21 DOI: 10.1016/bs.ctm.2021.08.002

Zhen Bouman Chen, Xuejing Liu, Aleysha T Chen

{"title":"\"Enhancing\" mechanosensing: Enhancers and enhancer-derived long non-coding RNAs in endothelial response to flow.","authors":"Zhen Bouman Chen, Xuejing Liu, Aleysha T Chen","doi":"10.1016/bs.ctm.2021.08.002","DOIUrl":"10.1016/bs.ctm.2021.08.002","url":null,"abstract":"Endothelial cells (ECs), uniquely localized and strategically forming the inner lining of vascular wall, constitute the largest cell surface by area in the human body. The dynamic sensing and response of ECs to mechanical cues, especially shear stress, is crucial for maintenance of vascular homeostasis. It is well recognized that different flow patterns associated with atheroprotective vs atheroprone regions in the arterial tree, result in distinct EC functional phenotypes with differential transcriptome profiles. Mounting evidence has demonstrated an integrative and essential regulatory role of non-coding genome in EC biology. In particular, recent studies have begun to reveal the importance of enhancers and enhancer-derived transcripts in flow-regulated EC gene expression and function. In this minireview, we summarize studies in this area and discuss examples in support of the emerging importance of enhancers and enhancer(-derived) long non-coding RNAs (elncRNAs) in EC mechanosensing, with a focus on flow-responsive EC transcription. Finally, we will provide perspective and discuss standing questions to elucidate the role of these novel regulators in EC mechanobiology.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39560068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanics of the cell: Interaction mechanisms and mechanobiological models. 细胞力学:相互作用机制和力学生物学模型。

4区生物学

Current topics in membranes Pub Date : 2020-01-01 Epub Date: 2020-10-02 DOI: 10.1016/bs.ctm.2020.09.001

Caleb Liebman, Andrew McColloch, Manoochehr Rabiei, Alan Bowling, Michael Cho

{"title":"Mechanics of the cell: Interaction mechanisms and mechanobiological models.","authors":"Caleb Liebman, Andrew McColloch, Manoochehr Rabiei, Alan Bowling, Michael Cho","doi":"10.1016/bs.ctm.2020.09.001","DOIUrl":"https://doi.org/10.1016/bs.ctm.2020.09.001","url":null,"abstract":"The importance of cell mechanics has long been recognized for the cell development and function. Biomechanics plays an important role in cell metabolism, regulation of mechanotransduction pathways and also modulation of nuclear response. The mechanical properties of the cell are likely determined by, among many others, the cytoskeleton elasticity, membrane tension and cell-substrate adhesion. This coordinated but complex mechanical interplay is required however, for the cell to respond to and influence in a reciprocal manner the chemical and mechanical signals from the extracellular matrix (ECM). In an effort to better and more fully understand the cell mechanics, the role of nuclear mechanics has emerged as an important contributor to the overall cellular mechanics. It is not too difficult to appreciate the physical connection between the nucleus and the cytoskeleton network that may be connected to the ECM through the cell membrane. Transmission of forces from ECM through this connection is essential for a wide range of cellular behaviors and functions such as cytoskeletal reorganization, nuclear movement, cell migration and differentiation. Unlike the cellular mechanics that can be measured using a number of biophysical techniques that were developed in the past few decades, it still remains a daunting challenge to probe the nuclear mechanics directly. In this paper, we therefore aim to provide informative description of the cell membrane and cytoskeleton mechanics, followed by unique computational modeling efforts to elucidate the nucleus-cytoskeleton coupling. Advances in our knowledge of complete cellular biomechanics and mechanotransduction may lead to clinical relevance and applications in mechano-diseases such as atherosclerosis, stem cell-based therapies, and the development of tissue engineered products.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/bs.ctm.2020.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25578643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Mechanobiology of microvesicle release, uptake, and microvesicle-mediated activation. 微泡释放、摄取和微泡介导激活的机械生物学。

4区生物学

Current topics in membranes Pub Date : 2020-01-01 Epub Date: 2020-09-10 DOI: 10.1016/bs.ctm.2020.08.004

Samantha C Schwager, Cynthia A Reinhart-King

{"title":"Mechanobiology of microvesicle release, uptake, and microvesicle-mediated activation.","authors":"Samantha C Schwager, Cynthia A Reinhart-King","doi":"10.1016/bs.ctm.2020.08.004","DOIUrl":"https://doi.org/10.1016/bs.ctm.2020.08.004","url":null,"abstract":"Microvesicles are small, membrane-bound vesicles that are shed from the plasma membrane of cells into the extracellular space. Microvesicles contain a variety of cargo not typically thought to be released from cells, including receptor tyrosine kinases, cytosolic signaling proteins, and microRNAs, which are transferred from donor cells to recipient cells. The transfer of microvesicle cargo can result in the transformation of recipient cells thereby supporting disease progression, including modified fibroblast metabolism, epithelial cell contractility, vascular remodeling, and immune cell inflammatory signaling. Additionally, microvesicles are believed to play prominent roles in cell-cell communication and disease progression as they are detected at elevated concentrations in diseased tissues. As microvesicle uptake by recipient cells can modulate cell function to promote disease progression, understanding the mechanisms and mechanosensitivity of microvesicle release, internalization, and the resulting signaling is crucial to fully comprehend their functions in disease. Here, we review recent advances in the understanding of actomyosin-regulated microvesicle biogenesis, microvesicle uptake via pinocytosis, and the resulting cellular transformation. We discuss the effects of altered cell contractility, mode of cell migration, and extracellular matrix compliance on microvesicle signaling, with direct implications in disease progression and identifying future therapeutic targets.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/bs.ctm.2020.08.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25578646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Membrane modulatory effects of omega-3 fatty acids: Analysis of molecular level interactions. omega-3脂肪酸的膜调节作用:分子水平相互作用的分析。

4区生物学

Current topics in membranes Pub Date : 2020-01-01 Epub Date: 2020-09-14 DOI: 10.1016/bs.ctm.2020.08.001

Manuela A A Ayee, Brendan C Bunker, Jordan L De Groot

引用次数: 4

Ion channels in capillary endothelium. 毛细血管内皮中的离子通道。

4区生物学

Current topics in membranes Pub Date : 2020-01-01 Epub Date: 2020-04-03 DOI: 10.1016/bs.ctm.2020.01.005

Daniela C G Garcia, Thomas A Longden

{"title":"Ion channels in capillary endothelium.","authors":"Daniela C G Garcia, Thomas A Longden","doi":"10.1016/bs.ctm.2020.01.005","DOIUrl":"https://doi.org/10.1016/bs.ctm.2020.01.005","url":null,"abstract":"Vascular beds are anatomically and functionally compartmentalized into arteries, capillaries, and veins. The bulk of the vasculature consists of the dense, anastomosing capillary network, composed of capillary endothelial cells (cECs) that are intimately associated with the parenchyma. Despite their abundance, the ion channel expression and function and Ca2+ signaling behaviors of capillaries have only recently begun to be explored in detail. Here, we discuss the established and emerging roles of ion channels and Ca2+ signaling in cECs. By mining a publicly available RNA-seq dataset, we outline the wide variety of ion channel genes that are expressed in these cells, which potentially imbue capillaries with a broad range of sensing and signal transduction capabilities. We also underscore subtle but critical differences between cEC and arteriolar EC ion channel expression that likely underlie key functional differences in ECs at these different levels of the vascular tree. We focus our discussion on the cerebral vasculature, but the findings and principles being elucidated in this area likely generalize to other vascular beds.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/bs.ctm.2020.01.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37931604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7