Current topics in membranes最新文献_第7页

The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC. 扩展工具箱研究lrrc8形成的体积调节阴离子通道VRAC。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-09 DOI: 10.1016/bs.ctm.2021.10.001

Yulia Kolobkova, Sumaira Pervaiz, Tobias Stauber

{"title":"The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC.","authors":"Yulia Kolobkova, Sumaira Pervaiz, Tobias Stauber","doi":"10.1016/bs.ctm.2021.10.001","DOIUrl":"https://doi.org/10.1016/bs.ctm.2021.10.001","url":null,"abstract":"The volume-regulated anion channel (VRAC) is activated upon cell swelling and facilitates the passive movement of anions across the plasma membrane in cells. VRAC function underlies many critical homeostatic processes in vertebrate cells. Among them are the regulation of cell volume and membrane potential, glutamate release and apoptosis. VRAC is also permeable for organic osmolytes and metabolites including some anti-cancer drugs and antibiotics. Therefore, a fundamental understanding of VRAC's structure-function relationships, its physiological roles, its utility for therapy of diseases, and the development of compounds modulating its activity are important research frontiers. Here, we describe approaches that have been applied to study VRAC since it was first described more than 30 years ago, providing an overview of the recent methodological progress. The diverse applications reflecting a compromise between the physiological situation, biochemical definition, and biophysical resolution range from the study of VRAC activity using a classic electrophysiology approach, to the measurement of osmolytes transport by various means and the investigation of its activation using a novel biophysical approach based on fluorescence resonance energy transfer.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":" ","pages":"119-163"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39690558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Mass spectrometry-based lipid analysis and imaging. 基于质谱的脂质分析和成像。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-09 DOI: 10.1016/bs.ctm.2021.10.005

Koralege C Pathmasiri, Thu T A Nguyen, Nigina Khamidova, Stephanie M Cologna

引用次数: 2

Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering. 用时间分辨的小角中子散射解译膜间和膜内的脂质转移。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-11 DOI: 10.1016/bs.ctm.2021.10.004

Ursula Perez-Salas, Sumit Garg, Yuri Gerelli, Lionel Porcar

引用次数: 1

Mechanotransduction in fibrosis: Mechanisms and treatment targets. 纤维化的机械转导:机制和治疗靶点。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-10-05 DOI: 10.1016/bs.ctm.2021.07.004

Chih-Fan Yeh, Caroline Chou, Kai-Chien Yang

引用次数: 2

Cardiovascular mechanosensitive ion channels-Translating physical forces into physiological responses. 心血管机械敏感离子通道——将物理力转化为生理反应。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-10-07 DOI: 10.1016/bs.ctm.2021.07.001

Ibra S Fancher

{"title":"Cardiovascular mechanosensitive ion channels-Translating physical forces into physiological responses.","authors":"Ibra S Fancher","doi":"10.1016/bs.ctm.2021.07.001","DOIUrl":"https://doi.org/10.1016/bs.ctm.2021.07.001","url":null,"abstract":"Cells and tissues are constantly exposed to mechanical stress. In order to respond to alterations in mechanical stimuli, specific cellular machinery must be in place to rapidly convert physical force into chemical signaling to achieve the desired physiological responses. Mechanosensitive ion channels respond to such physical stimuli in the order of microseconds and are therefore essential components to mechanotransduction. Our understanding of how these ion channels contribute to cellular and physiological responses to mechanical force has vastly expanded in the last few decades due to engineering ingenuities accompanying patch clamp electrophysiology, as well as sophisticated molecular and genetic approaches. Such investigations have unveiled major implications for mechanosensitive ion channels in cardiovascular health and disease. Therefore, in this chapter I focus on our present understanding of how biophysical activation of various mechanosensitive ion channels promotes distinct cell signaling events with tissue-specific physiological responses in the cardiovascular system. Specifically, I discuss the roles of mechanosensitive ion channels in mediating (i) endothelial and smooth muscle cell control of vascular tone, (ii) mechano-electric feedback and cell signaling pathways in cardiomyocytes and cardiac fibroblasts, and (iii) the baroreflex.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":" ","pages":"47-95"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39560072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

New Methods and Sensors for Membrane and Cell Volume Research 膜和细胞体积研究的新方法和传感器

4区生物学

Current topics in membranes Pub Date : 2021-01-01 DOI: 10.1016/s1063-5823(21)x0003-8

引用次数: 0

Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids. 利用Laurdan成像评价膜结构:氧化脂质破坏脂质包装。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-18 DOI: 10.1016/bs.ctm.2021.10.003

Irena Levitan

引用次数: 2

Fluorescence sensors for imaging membrane lipid domains and cholesterol. 荧光传感器成像膜脂结构域和胆固醇。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-10-20 DOI: 10.1016/bs.ctm.2021.09.004

Francisco J Barrantes

{"title":"Fluorescence sensors for imaging membrane lipid domains and cholesterol.","authors":"Francisco J Barrantes","doi":"10.1016/bs.ctm.2021.09.004","DOIUrl":"https://doi.org/10.1016/bs.ctm.2021.09.004","url":null,"abstract":"Lipid membrane domains are supramolecular lateral heterogeneities of biological membranes. Of nanoscopic dimensions, they constitute specialized hubs used by the cell as transient signaling platforms for a great variety of biologically important mechanisms. Their property to form and dissolve in the bulk lipid bilayer endow them with the ability to engage in highly dynamic processes, and temporarily recruit subpopulations of membrane proteins in reduced nanometric compartments that can coalesce to form larger mesoscale assemblies. Cholesterol is an essential component of these lipid domains; its unique molecular structure is suitable for interacting intricately with crevices and cavities of transmembrane protein surfaces through its rough β face while \"talking\" to fatty acid acyl chains of glycerophospholipids and sphingolipids via its smooth α face. Progress in the field of membrane domains has been closely associated with innovative improvements in fluorescence microscopy and new fluorescence sensors. These advances enabled the exploration of the biophysical properties of lipids and their supramolecular platforms. Here I review the rationale behind the use of biosensors over the last few decades and their contributions towards elucidation of the in-plane and transbilayer topography of cholesterol-enriched lipid domains and their molecular constituents. The challenges introduced by super-resolution optical microscopy are discussed, as well as possible scenarios for future developments in the field, including virtual (\"no staining\") staining.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":" ","pages":"257-314"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39943089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET. 用FRET研究出芽酵母细胞分裂和高渗胁迫过程中的分子拥挤。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-16 DOI: 10.1016/bs.ctm.2021.09.001

Sarah Lecinski, Jack W Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald, Mark C Leake

{"title":"Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET.","authors":"Sarah Lecinski, Jack W Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald, Mark C Leake","doi":"10.1016/bs.ctm.2021.09.001","DOIUrl":"10.1016/bs.ctm.2021.09.001","url":null,"abstract":"Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in their compositions and structures. However, it is not clear how these events are coordinated and how they integrate with regulation of molecular crowding. We use the budding yeast Saccharomyces cerevisiae as a model system to study these questions using recent progress in optical fluorescence microscopy and crowding sensing probe technology. We used a Förster Resonance Energy Transfer (FRET) based sensor, illuminated by confocal microscopy for high throughput analyses and Slimfield microscopy for single-molecule resolution, to quantify molecular crowding. We determine crowding in response to cellular growth of both mother and daughter cells, in addition to osmotic stress, and reveal hot spots of crowding across the bud neck in the burgeoning daughter cell. This crowding might be rationalized by the packing of inherited material, like the vacuole, from mother cells. We discuss recent advances in understanding the role of crowding in cellular regulation and key current challenges and conclude by presenting our recent advances in optimizing FRET-based measurements of crowding while simultaneously imaging a third color, which can be used as a marker that labels organelle membranes. Our approaches can be combined with synchronized cell populations to increase experimental throughput and correlate molecular crowding information with different stages in the cell cycle.","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":" ","pages":"75-118"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7612257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39943093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane tension. 膜张力。

4区生物学

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-02 DOI: 10.1016/bs.ctm.2021.09.002

Pei-Chuan Chao, Frederick Sachs

引用次数: 1