利用Laurdan成像评价膜结构:氧化脂质破坏脂质包装。

4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-18 DOI:10.1016/bs.ctm.2021.10.003

Irena Levitan

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引用次数: 2

摘要

不同脂质对膜结构/脂质顺序的影响对多种生物过程至关重要。劳氏显微镜提供了一个独特的工具来评估这种性质在异质生物膜。本文综述了该方法的一般原理及其在模型膜和细胞中的应用。它还提供了一个深入的讨论，利用Laurdan显微镜来评估胆固醇、氧化甾醇和氧化磷脂对血管内皮细胞中有序和无序结构域的脂质堆积的不同影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids.

Impact of different lipids on membrane structure/lipid order is critical for multiple biological processes. Laurdan microscopy provides a unique tool to assess this property in heterogeneous biological membranes. This review describes the general principles of the approach and its application in model membranes and cells. It also provides an in-depth discussion of the insights obtained using Laurdan microscopy to evaluate the differential effects of cholesterol, oxysterols and oxidized phospholipids on lipid packing of ordered and disordered domains in vascular endothelial cells.

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来源期刊

Current topics in membranes 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Current Topics in Membranes provides a systematic, comprehensive, and rigorous approach to specific topics relevant to the study of cellular membranes. Each volume is a guest edited compendium of membrane biology.