Cytotechnology最新文献

{"title":"Use of peripheral blood platelet lysate as a rich source of growth factors for cultivation of fastidious primary vascular cells and comparison with cell-conditioned medium.","authors":"Shayan Amanzadeh, Amir Amanzadeh, Mohammad Ali Shokrgozar, Mehrdad Moosazadeh Moghaddam","doi":"10.1007/s10616-026-01044-1","DOIUrl":"https://doi.org/10.1007/s10616-026-01044-1","url":null,"abstract":"<p><p>The optimization of culture conditions for primary vascular cells remains challenging, as cell viability alone does not necessarily reflect preserved molecular identity or functional stability. Human umbilical vein endothelial cells (HUVECs) and Human aortic vascular smooth muscle cells (T/G HA-VSMCs) are highly sensitive to their microenvironment, and non-specific or poorly standardized supplements may sustain survival while disrupting gene expression and phenotypic integrity. Human platelet lysate (HPL) has emerged as a human-derived alternative to conventional supplements. However, its role as a supplement rather than a complete serum replacement remains insufficiently explored in vascular cell culture systems. This research innovates by evaluating HPL and CM as complementary supplements to FBS rather than standard specific medium. HUVECs and T/G HA-VSMC were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and either 5% or 10% HPL or fibroblast-derived conditioned medium (CM). Cell viability was assessed using MTT assay, morphological characteristics were evaluated by phase-contrast microscopy, and lineage-specific gene expression was analyzed by quantitative real-time PCR targeting endothelial (CD31, CD144) and smooth muscle (ACTA2, SM22α) markers. Platelet lysates derived from both fresh and expired platelet concentrates were evaluated. HPL supplementation significantly enhanced cell viability and preserved characteristic morphology in both cell types compared to CM. Importantly, no significant alterations in lineage-specific gene expression were observed following HPL supplementation, indicating maintenance of cellular identity. Comparable biological outcomes were obtained using HPL prepared from fresh and expired platelet concentrates, suggesting retained growth factor activity beyond clinical transfusion applicability. Human platelet lysate, when used as a supplement to fetal bovine serum, supports the growth and viability of human umbilical vein endothelial cells and vascular smooth muscle cells while preserving the expression of key phenotypic markers. Compared to conditioned media, HPL demonstrated more stable effects on gene expression and cell morphology. Additionally, the use of expired platelet concentrates provides a cost-effective and practical approach for cell culture applications.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"175"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13469017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LINC01094/miR-128-3p axis-mediated regulation of neuronal apoptosis and inflammation in post-intracerebral hemorrhage brain injury.","authors":"Da Yang, Yilin Feng, Min Yang, Yawen Zhang, Zhipeng Liu","doi":"10.1007/s10616-026-01055-y","DOIUrl":"10.1007/s10616-026-01055-y","url":null,"abstract":"<p><p>Post-intracerebral hemorrhage brain injury (post-ICH BI), a cerebrovascular subtype, has limited early diagnostic markers and difficulties in controlling secondary injury. This study evaluated serum LINC01094 for early diagnosis of post-ICH BI and its regulation of neuronal apoptosis and inflammation via targeting miR-128-3p. Eighty-nine patients with early post-ICH BI and 106 controls with high-risk factors underwent qPCR to measure serum LINC01094 and miR-128-3p levels. ROC curve, logistic regression, and correlation analyses were used to assess their clinical value. A post-ICH BI cell model was established using OGD/R combined with Hemin-CM, and the effects of LINC01094 silencing alone or co-inhibition of miR-128-3p were evaluated via qPCR, CCK-8, Western blot, and ELISA. Serum LINC01094 was significantly increased in post-ICH BI patients, while miR-128-3p was decreased. A strong negative correlation was observed between the two. The AUC for serum LINC01094 in diagnosing post-ICH BI was 0.877. High LINC01094 expression was an independent risk factor for disease onset and positively correlated with hemorrhage volume and NIHSS score, but negatively correlated with GCS score. In cell experiments, LINC01094 silencing enhanced SH-SY5Y proliferation, reduced pro-apoptotic proteins (Bax, p38α, caspase-3), suppressed pro-inflammatory cytokines (IL-1β, TNF-α, IL-6), increased SOD and CAT activity, and lowered NSE and S100B levels. Co-inhibition of miR-128-3p reversed the protective effects of LINC01094 silencing. LINC01094 serves as a potential biomarker for early diagnosis and severity assessment of post-ICH BI. In vitro evidence suggests the LINC01094/miR-128-3p axis may contribute to neuronal injury, offering valuable new insights for clinical management.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at https://doi.org/10.1007/s10616-026-01055-y.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"187"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13522314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148850036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stichopus japonicus acidic mucopolysaccharide (SJAMP) exerts the tumor-suppressive activity in cervical cancer by targeting TFAP2A to downregulate CENPW.","authors":"Zhengxiang Cai, Dachun Liu, Yongpeng He, Zhanglan Fang, Huguang Yang, Xia Lan, Miao He, Yi Xian, Juan Gong","doi":"10.1007/s10616-026-01023-6","DOIUrl":"https://doi.org/10.1007/s10616-026-01023-6","url":null,"abstract":"<p><p>Cervical cancer (CC) remains a leading cause of cancer-related mortality among women, and advances in its therapeutic approaches are disappointing, in which novel therapies and precise experimental approaches are needed to be exploited. This study intends to explore the effects and involved molecular mechanism of Stichopus japonicus acidic mucopolysaccharide (SJAMP) on CC progression. CCK8 and EdU assays were utilized to test the ability of cell proliferation. Colony formation, transwell, flow cytometry assays were applied to detect cell colonies, migration, invasion, and apoptosis accordingly. RT-qPCR and western blot were used to quantify the expression levels of transcription factor AP-2 Alpha (TFAP2A) and centromere protein W (CENPW), respectively. JASPAR website, ChIP-qPCR, and dual-luciferase reporter assay were used to verify the binding loci of TFAP2A on the promoter region of CENPW. Xenograft tumor models were produced to confirm the effects of CENPW silence or SJAMP treatment in CC. SJAMP suppressed malignant phenotypes of CC cells in a dosage-dependent manner. Besides, SJAMP regulated the biological behaviors of CC cells via regulating TFAP2A expression, which further regulated CENPW expression as its transcription factor. The expression of CENPW was elevated in CC and positively correlated with TFAP2A, and the silence of CENPW hampered tumor growth in vivo. In addition, CENPW overexpression abolished the repressed cell malignant activities induced by TFAP2A silence. Xenograft tumor models proved that SJAMP curbed tumor growth in vivo via repressing TFAP2A-mediated CENPW expression. Our research revealed that SJAMP exerted its anti-tumor effects via decreasing TFAP2A-mediated CENPW expression in CC.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-026-01023-6.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"165"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13400524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRIM39 suppresses endometrioid endometrial carcinoma progression via ubiquitination and degradation of HOXB9.","authors":"Fei Zheng, Shuya Han, Hongqing Gu, Shaozheng Chen","doi":"10.1007/s10616-026-01033-4","DOIUrl":"10.1007/s10616-026-01033-4","url":null,"abstract":"<p><p>Endometrioid endometrial carcinoma (EEC) is the major histological type of endometrial cancer, the most common gynecological malignancies in the world, and its incidence and mortality continue to increase. However, the molecular mechanism of its occurrence and development has not been fully elucidated, which limits the development of effective treatment strategies. By integrating transcriptome data from multiple public databases and validation analysis of clinical samples, we found for the first time that E3 ubiquitin ligase TRIM39 was significantly and universally down-regulated in EEC tissues. Further functional experiments in vitro and in vivo showed that restoring TRIM39 expression effectively inhibited tumor cell proliferation, colony formation ability, migration and invasion, and reversed epithelial-mesenchymal transition (EMT) process. Mechanistically, through co-immunoprecipitation and ubiquitination analysis, we revealed that TRIM39 directly binds to and mediates the K48-linked polyubiquitination of HOXB9, which promotes its proteasomal degradation. Critical rescue experiments confirmed that re-overexpression of HOXB9 partially reversed TRIM39-mediated tumor suppression. Taken together, this study not only identifies TRIM39 as an important novel tumor suppressor in EEC for the first time, but also illustrates a novel \"TRIM39-HOXB9\" protein stability axis, which provides new molecular insights into understanding the progression of EEC. TRIM39 was identified as a potential therapeutic target and prognostic biomarker for the disease.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-026-01033-4.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"170"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13427696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artesunate promotes liver regeneration by modulating mitophagy via the AMPKα1/2/mTOR signaling pathway.","authors":"Xinkai Li, Chuntao Li, Chao Fang, Yong Guo, Changyou Lu","doi":"10.1007/s10616-026-01039-y","DOIUrl":"https://doi.org/10.1007/s10616-026-01039-y","url":null,"abstract":"<p><p>Partial hepatectomy (PH) triggers acute liver insufficiency and a tightly-orchestrated regenerative program. Here we show that artesunate (ART) markedly accelerates liver mass restoration in mice subjected to PH. ART elevated the liver index, normalized serum total protein and albumin, and curbed ALT/AST leakage. Histologically, ART attenuated hepatocellular necrosis and reduced lesion scores in a dose-dependent manner. Mechanistically, ART amplified Ki67-positive hepatocytes, suppressed apoptosis, and shifted cell-cycle distribution from G1 arrest toward G2/M progression. These regenerative effects were associated with enhanced mitophagy-related changes, as evidenced by increased TOM20/LC3B co-localization, elevated LC3-II/I ratio, increased PINK1 and Parkin expression, and reduced p62 accumulation. A chloroquine-based lysosomal inhibition assay further supported that ART enhanced mitophagy-associated flux. Docking analysis suggested a potential interaction between ART and AMPKα1/2, with docking energies of - 6.41 and - 6.45 kcal mol^- 1 for PRKAA1 and PRKAA2, respectively. ART increased p-AMPKα1/2 and suppressed mTOR signaling, as further supported by reduced p-S6 expression. Pharmacological inhibition of AMPK with Compound C attenuated ART-induced PINK1/Parkin-associated mitophagy-related signaling, suppressed ART-enhanced hepatocyte proliferation, increased apoptosis, and partially reversed the ART-mediated reduction in p-S6 expression. Collectively, these findings suggest that ART promotes post-PH liver regeneration by activating AMPKα1/2-associated signaling and enhancing mitophagy-associated flux, positioning ART as a potential adjunct strategy for liver regeneration after hepatectomy.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"166"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13400547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CircRNA PVT1 promotes migration, invasion and glucose metabolism of thyroid cancer cells through modulating the miR-195-5p-<i>PDK4</i> axis.","authors":"Huanli Hu, Yanan Lu, Yingchuan Sun, Xue Liu","doi":"10.1007/s10616-026-00960-6","DOIUrl":"https://doi.org/10.1007/s10616-026-00960-6","url":null,"abstract":"<p><p>This study aimed to investigate the biological functions and underlying molecular mechanisms of circular RNA plasmacytoma variant translocation 1 (circPVT1) in the progression of thyroid cancer.The expression levels of circPVT1 in thyroid tumor tissues and cell lines were compared to normal controls, and its association with clinicopathological features was analyzed. Functional assays were performed to assess the effects of circPVT1 silencing on thyroid cancer cell migration, invasion, and glucose metabolism. The interaction between circPVT1 and miR-195-5p was validated using bioinformatics prediction, RNA pull-down, dual-luciferase reporter assays, and RNA fluorescence in situ hybridization (FISH). The target relationship between miR-195-5p and pyruvate dehydrogenase kinase 4 (PDK4) was examined via luciferase reporter and Western blot assays. Rescue and epistasis experiments were conducted by restoring miR-195-5p expression or knocking down PDK4 in circPVT1-overexpressing cells. The role of the circPVT1/miR-195-5p/PDK4 axis was further validated in vivo using subcutaneous xenograft and experimental lung metastasis models.circPVT1 was significantly upregulated in thyroid tumors and cell lines, and its high expression correlated with advanced TNM stage and lymph node metastasis. Silencing circPVT1 suppressed migration, invasion, and glycolytic metabolism of thyroid cancer cells. Mechanistically, circPVT1 acted as a sponge for miR-195-5p, downregulating its expression, and miR-195-5p directly targeted and inhibited PDK4. Restoration of miR-195-5p or knockdown of PDK4 reversed the oncogenic phenotypes induced by circPVT1 overexpression. In vivo, circPVT1 promoted tumor growth and lung metastasis, which were attenuated by co-expression of miR-195-5p. Molecular analysis of lung metastases confirmed the dysregulation of the circPVT1/miR-195-5p/PDK4 axis within metastatic lesions.CircPVT1 promotes migration, invasion, glycolytic metabolism, and metastasis in thyroid cancer by sponging miR-195-5p and upregulating PDK4. These findings highlight the circPVT1/miR-195-5p/PDK4 axis as a potential therapeutic target for thyroid cancer intervention.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-026-00960-6.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"176"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epimedium flavonoids alleviate the demyelination and neurological disorders in mice exposed to Cuprizone through the RIPK1/RIPK3/MLKL pathway.","authors":"Denglei Ma, Dongyang Zhou, Weipeng Wei, Xuanyu Luan, Lan Zhang, Lin Li","doi":"10.1007/s10616-026-01042-3","DOIUrl":"10.1007/s10616-026-01042-3","url":null,"abstract":"<p><p>Demyelination is a hallmark of many neurological diseases such as Multiple sclerosis. The cuprizone (CPZ) model is as an optical model for the investigation of white matter lesions (WMLs). In this study, the treatment effects and mechanisms of Epimedium flavonoids (EF) on the CPZ-induced demyelination model were explored. After feeding chow containing 0.2% CPZ for 3 weeks, female C57BL/6 mice were administered with EF for another 4 weeks. Behavioral tests were conducted after treatment. MRI-diffusion tensor imaging, transmission electron microscopy, and histological staining were applied to compare the changes in white matter. Immunohistochemical staining and western blot were applied to detect protein expressions. Our findings indicated that EF significantly improved cognitive functions, depressive-like behavior and WMLs of the CPZ mice. More integrated myelin fibers and increased mature oligodendrocytes were observed in EF-treated CPZ mice. These improvements were found to be associated with inhibiting the activation of microglia and astrocyte, and reducing the expression levels of receptor-interacting protein kinase-1 (RIPK1), p-RIPK3 and mixed lineage kinase domain-like protein (MLKL) expression. In conclusion, EF alleviated CPZ-induced cognitive impairment, depressive-like behavior disorders, and WMLs in this model, by promoting oligodendrocyte differentiation and maturation, and inhibiting the activation of glia cells and RIPK1/RIPK3/MLKL signaling pathway. EF showed protective effects on demyelination, and therefore are promising agents for demyelinating diseases.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"174"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13461985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico and in vitro evaluation of synergistic anticancer effects of erucin and kaempferol from <i>Eruca sativa</i> against human A549 non-small cell lung cancer cells (NSCLC).","authors":"Akshay Kumar, Nakul Bhardwaj, Rahul Sharma, Palak Thakur, Karanvir Singh, Ashun Chaudhary, Sukhraj Kaur, Saroj Arora, Astha Bhatia","doi":"10.1007/s10616-026-01049-w","DOIUrl":"https://doi.org/10.1007/s10616-026-01049-w","url":null,"abstract":"<p><p>Dietary phytochemicals exhibit anticancer activity, and increasing evidence suggests that the combinations of co-occurring compounds may provide greater therapeutic efficacy than individual compounds. <i>Eruca sativa</i> Mill. (rocket salad) contains both erucin and kaempferol; however, their synergistic anticancer activity against non-small cell lung cancer (NSCLC) has not been systematically investigated. Network pharmacology, molecular docking, and molecular dynamics (MD) simulations were performed to identify molecular targets of erucin and kaempferol. Their synergistic anticancer activity was subsequently evaluated in A549 (NSCLC) cells using the MTT assay and the combination index (CI) analysis, followed by a cell migration assay, fluorescence microscopy, flow cytometry, biochemical assays, caspase-3/7 assay, and RT-qPCR to investigate the underlying mechanisms. The combination of erucin (20 µM) and kaempferol (40 µM) exhibited the strongest synergistic interaction (Combination Index, CI = 0.428), significantly inhibiting A549 cell growth and migration while exhibiting preferential cytotoxicity toward A549 cells with minimal effects on WI-38 lung fibroblasts. Combined treatment increased overall oxidative stress, induced mitochondrial membrane depolarisation, enhanced caspase-3/7 activity and apoptosis, and modulated Bax/Bcl-2 gene expression, indicating activation of the intrinsic mitochondrial apoptotic pathway. Computational analyses identified EGFR as a potential molecular target that may contribute to the observed anticancer effects. The erucin-kaempferol combination demonstrated synergistic anticancer activity against A549 NSCLC cells, highlighting the bioactive potential of naturally co-occurring <i>E. sativa</i>-derived phytochemicals. However, these findings are limited to computational and in vitro studies, and in vivo studies are required to validate their therapeutic efficacy and elucidate the underlying molecular mechanisms.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-026-01049-w.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"182"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148789310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticle using <i>Hemidesmus indicus</i> (L) R. Br. root extract and cytotoxicity assessment against ovarian cancer cell line (SKOV3).","authors":"Jayachandran Halka, Gayathiri Gunasangkaran, Kumaresan Kowsalya, Anjali K Ravi, M Subhashini, Muthukrishnan Saradhadevi, Packiaraj Gurusaravanan, Muthuswamy Razia, Muthukrishnan Anand, Muthukrishnan Arun","doi":"10.1007/s10616-026-01029-0","DOIUrl":"10.1007/s10616-026-01029-0","url":null,"abstract":"<p><p><i>Hemidesmus indicus</i> (L) R. Br. root extract-mediated silver nanoparticles (HiAgNPs) were synthesized and evaluated for anticancer activity against ovarian cancer cells. GC-MS analysis identified forty bioactive compounds in the root extract, which facilitated the green synthesis of nanoparticles. Characterization using UV-visible spectroscopic analysis showed a surface plasmon resonance peak at 428 nm, while SEM revealed predominantly spherical nanoparticles. On the other hand, DLS revealed a size distribution peak at 222.2 d.nm, whereas zeta potential was found to be -20.9 mV. Further, EDX analysis confirmed the presence of silver (Ag) and oxygen (O), and FTIR revealed distinct patterns in the stretching vibrations of their functional groups, indicating the presence of capping agents in the nanoparticles. Antioxidant activity was assessed using DPPH and FRAP assays. MTT assay demonstrated significant dose- and time-dependent cytotoxicity of HiAgNPs against SKOV3 cells. Apoptotic induction was confirmed through nuclear morphology, acridine orange/ethidium bromide staining, migration assays, and RT-PCR analysis of <i>Bax</i>, <i>Bcl2</i> and <i>Caspase 3</i> genes. Overall, this study highlights HiAgNPs as a promising eco-friendly therapeutic candidate for treating ovarian cancer .</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 5","pages":"191"},"PeriodicalIF":2.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13526738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Based on comprehensive analysis of ScRNA-seq and bulk RNA-seq, identification of palmitoylation-related genes as biomarkers to evaluate prognosis and immune landscape in stomach adenocarcinoma.","authors":"Zeren Leng, Wenzhe Zhang","doi":"10.1007/s10616-026-01014-7","DOIUrl":"https://doi.org/10.1007/s10616-026-01014-7","url":null,"abstract":"<p><p>Stomach adenocarcinoma (STAD) poses a significant global health burden, with notable geographic variations in its incidence and complex underlying causes. Palmitoylation plays a crucial role in cancer development, yet its prognostic relevance in STAD remains poorly understood. Multi-omics data integration was performed using TCGA-STAD (N=407) and GEO-GSE26901 (N=110) cohorts with single-cell data (GSE167297; N=14 samples). Palmitoylation-related genes (PRGs=3,599) from GeneCards underwent differential expression screening and Cox regression to identify prognostic DEPRGs. A LASSO-multivariate Cox model constructed a 5-gene signature for risk stratification, validated externally. Comprehensive analyses included molecular subtyping (ConsensusClusterPlus), immune infiltration (CIBERSORT/ssGSEA), immunotherapy response (TIDE/IPS), drug sensitivity (pRRophetic/CellMiner), pathway enrichment (GSEA/clusterProfiler), and single-cell ecosystem characterization. Based on multi-omics analysis of STAD, we identified 5 signature gene (GAMT, RDH12, RNASE1, SERPINE1, and SLC52A3) to construct a robust prognostic model. High-risk group exhibited enrichment in pro-metastatic pathways (CAMs, ECM-receptor interaction) and an immunosuppressive microenvironment (increased M2 macrophages, decreased CD8⁺ T cells), correlating with poor survival, higher TIDE scores, and immunotherapy resistance. Consensus clustering revealed two molecular subtypes with divergent survival outcomes. Single-cell analysis revealed SERPINE1⁺ endothelial cells as a high-risk subpopulation driving angiogenesis via MK pathway interactions with epithelial/stromal cells. Based on multi-omics integration, this study establishes PRGs signature as a robust prognostic biomarker for STAD, enabling risk stratification, molecular subtyping, and prediction of immunotherapy response while elucidating PRGs-mediated remodeling of the tumor-immune microenvironment.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-026-01014-7.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"78 4","pages":"143"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13309609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148344058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}