Anti-cancer efficiency of Campylobacter jejuni secretome loaded chitosan nanoparticles on colorectal cancer signaling pathways.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2025-06-01 Epub Date: 2025-04-28 DOI:10.1007/s10616-025-00756-0

Ghazale Khodadadi, Masoumeh Saberpour, Bita Bakhshi, Sara Minaeian

{"title":"Anti-cancer efficiency of Campylobacter jejuni secretome loaded chitosan nanoparticles on colorectal cancer signaling pathways.","authors":"Ghazale Khodadadi, Masoumeh Saberpour, Bita Bakhshi, Sara Minaeian","doi":"10.1007/s10616-025-00756-0","DOIUrl":null,"url":null,"abstract":"Colorectal cancer (CRC) remains as a major health problem with high lethality rate in the world. The innovate therapeutic strategies are essential in CRC management. The purpose of this research was to evaluate the effect of chitosan nanoparticle containing Campylobacter jejuni culture supernatant (CNP/Cj-sup) on genes involved in CRC signaling pathways. CNP/Cj-sup was fabricated via ionotropic gelation method. Dynamic light scattering and transmission electron microscopy (TEM) techniques were employed to characterize of the CNP/Cj-sup including the electrical charge, size distribution, and morphological properties. The loaded protein, released protein, and entrapment efficacy (EE) were assayed utilizing a BCA assay kit. After the evaluation of the viability of Caco-2 (colon adenocarcinoma) and HDF (human dermal fibroblasts) cells against CNP/Cj-sup by MTT assay, subsequently anti-tumor effect of CNP/Cj-sup on genes associated with CRC signaling pathways was assessed via real-time PCR method. The size dispersion of CNP/Cj-sup was 400.6 ± 24.4 nm with an electrical charge of + 4.5 mV. The loaded protein was calculated 1100 µg. The release rate of protein from CNP/Cj-sup was 78% at pH of 6.8 after 48 h, with EE of 74.62%. The viability of Caco-2 and HDF cells against CNP/Cj-sup (1100 µg + 0.05%) was measured 75.8 and 96.5%, respectively after 48 h. CNP/Cj-sup exhibited the highest efficacy in inhibiting the expression of oncogenes TGF-α, Bcl2, TLR4, CEA, TGF-β, and PI3K by to 0.06, 0.34, 0.14, 0.13, 0.08, and 0.14-fold (p value < .0001). Moreover, it led to a significant increase in the expression of the suppressor genes caspase9 and PTEN by to 55.7 and 1.8- fold (p value < .0001). CNP/Cj-sup demonstrated the highest efficiency in suppressing TGF-α and enhancing caspase9 compared to CNP and Cj-sup. In conclusion, CNP/Cj-sup as an innovative potential anticancer agent, with the ability to modulate genes involved in CRC progression, represents a promising approach to CRC treatment.Graphical abstract: The effect of CNP/Cj-sup on different colorectal cancer signaling pathways.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"93"},"PeriodicalIF":2.0000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037461/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytotechnology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10616-025-00756-0","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/28 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Colorectal cancer (CRC) remains as a major health problem with high lethality rate in the world. The innovate therapeutic strategies are essential in CRC management. The purpose of this research was to evaluate the effect of chitosan nanoparticle containing Campylobacter jejuni culture supernatant (CNP/Cj-sup) on genes involved in CRC signaling pathways. CNP/Cj-sup was fabricated via ionotropic gelation method. Dynamic light scattering and transmission electron microscopy (TEM) techniques were employed to characterize of the CNP/Cj-sup including the electrical charge, size distribution, and morphological properties. The loaded protein, released protein, and entrapment efficacy (EE) were assayed utilizing a BCA assay kit. After the evaluation of the viability of Caco-2 (colon adenocarcinoma) and HDF (human dermal fibroblasts) cells against CNP/Cj-sup by MTT assay, subsequently anti-tumor effect of CNP/Cj-sup on genes associated with CRC signaling pathways was assessed via real-time PCR method. The size dispersion of CNP/Cj-sup was 400.6 ± 24.4 nm with an electrical charge of + 4.5 mV. The loaded protein was calculated 1100 µg. The release rate of protein from CNP/Cj-sup was 78% at pH of 6.8 after 48 h, with EE of 74.62%. The viability of Caco-2 and HDF cells against CNP/Cj-sup (1100 µg + 0.05%) was measured 75.8 and 96.5%, respectively after 48 h. CNP/Cj-sup exhibited the highest efficacy in inhibiting the expression of oncogenes TGF-α, Bcl2, TLR4, CEA, TGF-β, and PI3K by to 0.06, 0.34, 0.14, 0.13, 0.08, and 0.14-fold (p value < .0001). Moreover, it led to a significant increase in the expression of the suppressor genes caspase9 and PTEN by to 55.7 and 1.8- fold (p value < .0001). CNP/Cj-sup demonstrated the highest efficiency in suppressing TGF-α and enhancing caspase9 compared to CNP and Cj-sup. In conclusion, CNP/Cj-sup as an innovative potential anticancer agent, with the ability to modulate genes involved in CRC progression, represents a promising approach to CRC treatment.

Graphical abstract: The effect of CNP/Cj-sup on different colorectal cancer signaling pathways.

查看原文本刊更多论文

空肠弯曲菌分泌组负载壳聚糖纳米颗粒对结直肠癌信号通路的抗癌作用。

结直肠癌（CRC）仍然是世界范围内死亡率高的主要健康问题。创新治疗策略对结直肠癌的治疗至关重要。本研究旨在探讨空肠弯曲菌培养上清（CNP/Cj-sup）纳米壳聚糖对结直肠癌信号通路相关基因的影响。采用离子化凝胶法制备CNP/Cj-sup。采用动态光散射和透射电子显微镜（TEM）技术对CNP/Cj-sup进行了电荷、尺寸分布和形貌表征。利用BCA检测试剂盒检测负载蛋白、释放蛋白和包封效率（EE）。MTT法检测Caco-2（结肠腺癌）和HDF（人真皮成纤维细胞）细胞对CNP/Cj-sup的抗肿瘤活性后，real-time PCR法检测CNP/Cj-sup对结直肠癌信号通路相关基因的抗肿瘤作用。CNP/Cj-sup的色散尺寸为400.6±24.4 nm，电荷为+ 4.5 mV。载蛋白量为1100µg。在pH为6.8的条件下，CNP/Cj-sup在48 h后的蛋白质释放率为78%，EE为74.62%。CNP/Cj-sup（1100µg + 0.05%）作用48 h后，Caco-2和HDF细胞的活力分别为75.8和96.5%。CNP/Cj-sup对癌基因TGF-α、Bcl2、TLR4、CEA、TGF-β和PI3K的抑制效果最高，分别为0.06、0.34、0.14、0.13、0.08和0.14倍（p值为caspase9和PTEN），分别为55.7和1.8倍（p值为TGF-α）和增强caspase9。综上所述，CNP/Cj-sup作为一种创新的潜在抗癌药物，具有调节参与CRC进展的基因的能力，代表了一种有希望的CRC治疗方法。图形摘要：CNP/Cj-sup对不同结直肠癌信号通路的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.