Cytotechnology最新文献_第7页

Pulsed electrical stimulation and amino acid derivatives promote collagen gene expression in human dermal fibroblasts 脉冲电刺激和氨基酸衍生物促进人真皮成纤维细胞胶原基因表达

4区生物学

Cytotechnology Pub Date : 2023-11-09 DOI: 10.1007/s10616-023-00604-z

Hiroya Urabe, Ryuji Akimoto, Shohei Kamiya, Katsu Hosoki, Hideyuki Ichikawa, Toshio Nishiyama

引用次数: 0

Nitric oxide-cyclic GMP role in Ang II induced hyperpolarization in bovine aortic endothelium cell line (BAE-1) 一氧化氮-环GMP在angii诱导的牛主动脉内皮细胞系(ae -1)超极化中的作用

4区生物学

Cytotechnology Pub Date : 2023-11-02 DOI: 10.1007/s10616-023-00602-1

Chinar M. Mohammed, Omar A. M. Al-Habib

引用次数: 0

Study on the mechanism of CXCL12/CXCR4-axis-mediated upregulation of IL-8 and IL-6 on the biological function of acute T lymphocyte leukaemia cells CXCL12/ cxcr4轴介导的IL-8、IL-6上调对急性T淋巴细胞白血病细胞生物学功能的影响机制研究

4区生物学

Cytotechnology Pub Date : 2023-10-28 DOI: 10.1007/s10616-023-00600-3

Liping Zhou, Hui Zhao, Chao Zhang, Zhe Chen, Dong Li, Guanglei Qian

引用次数: 0

Construction of a novel kinetic model for the production process of a CVA6 VLP vaccine in CHO cells CHO细胞CVA6 VLP疫苗生产过程新动力学模型的建立

4区生物学

Cytotechnology Pub Date : 2023-10-27 DOI: 10.1007/s10616-023-00598-8

Zhou Xing, Thao Bich Nguyen, Guirong Kanai-Bai, Noriko Yamano-Adachi, Takeshi Omasa

{"title":"Construction of a novel kinetic model for the production process of a CVA6 VLP vaccine in CHO cells","authors":"Zhou Xing, Thao Bich Nguyen, Guirong Kanai-Bai, Noriko Yamano-Adachi, Takeshi Omasa","doi":"10.1007/s10616-023-00598-8","DOIUrl":"https://doi.org/10.1007/s10616-023-00598-8","url":null,"abstract":"Abstract Bioprocess development benefits from kinetic models in many aspects, including scale-up, optimization, and process understanding. However, current models are unable to simulate the production process of a coxsackievirus A6 (CVA6) virus-like particle (VLP) vaccine using Chinese hamster ovary cell culture. In this study, a novel kinetic model was constructed, correlating (1) cell growth, death, and lysis kinetics, (2) metabolism of major metabolites, and (3) CVA6 VLP production. To construct the model, two batches of a laboratory-scale 2 L bioreactor cell culture were prepared and various pH shift strategies were applied to examine the effect of pH shift. The proposed model described the experimental data under various conditions with high accuracy and quantified the effect of pH shift. Next, cell culture performance with various pH shift timings was predicted by the calibrated model. A trade-off relationship was found between product yield and quality. Consequently, multiple objective optimization was performed by integrating desirability methodology with model simulation. Finally, the optimal operating conditions that balanced product yield and quality were predicted. In general, the proposed model improved the process understanding and enabled in silico process development of a CVA6 VLP vaccine.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136262608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosome-mediated circGMPS facilitates the development of gastric cancer cells through miR-144-3p/PUM1 外泌体介导的circGMPS通过miR-144-3p/PUM1促进胃癌细胞的发育

4区生物学

Cytotechnology Pub Date : 2023-10-20 DOI: 10.1007/s10616-023-00597-9

Yuexin Zhang, Wenrui Xie, Wenhong Zheng, Xiaoying Qian, Chengwei Deng

引用次数: 0

Reconstruction of ovarian follicular-like structure by recellularization of a cell-free human ovarian scaffold with mouse fetal ovarian cells 用小鼠胚胎卵巢细胞重建无细胞人卵巢支架重建卵巢卵泡样结构

4区生物学

Cytotechnology Pub Date : 2023-10-06 DOI: 10.1007/s10616-023-00595-x

Maryam Nezhad Sistani, Saeed Zavareh, Mojtaba Rezazadeh Valojerdi, Mojdeh Salehnia

引用次数: 0

PRPF19 functions in DNA damage repair and gemcitabine sensitivity via regulating DDB1 in bladder cancer cells PRPF19通过调控膀胱癌细胞中的DDB1参与DNA损伤修复和吉西他滨敏感性

4区生物学

Cytotechnology Pub Date : 2023-10-05 DOI: 10.1007/s10616-023-00599-7

Jingjiang Yu, Shuxiong Ge

引用次数: 0

Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments. 在多种氧气环境下评估MTT和磺基罗丹明B细胞增殖测定。

IF 2 4区生物学

Cytotechnology Pub Date : 2023-10-01 Epub Date: 2023-07-05 DOI: 10.1007/s10616-023-00584-0

Ming Yao, Glenn Walker, Michael P Gamcsik

{"title":"Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments.","authors":"Ming Yao, Glenn Walker, Michael P Gamcsik","doi":"10.1007/s10616-023-00584-0","DOIUrl":"10.1007/s10616-023-00584-0","url":null,"abstract":"Cell proliferation can be measured directly by counting cells or indirectly using assays that quantitate total protein or metabolic activity. However, for comparing cell proliferation under varying oxygen conditions it is not clear that these assays are appropriate surrogates for cell counting as cell metabolism and protein synthesis may vary under different oxygen environments. We used permeable bottom tissue culture ware to compare proliferation assays as a function of static oxygen concentrations under oxygen partial pressure (pO2) levels ranging from 2 to 139 mmHg. Cell proliferation was measured by cell counting and compared to surrogate methods measuring cell metabolism (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT) and total protein (sulforhodamine B) assays under these different environments in Caco-2, MCF-7, MCF-10A and PANC-1 human cell lines. We found that the MTT readings do not correlate with cell number for the Caco-2 and PANC-1 cell lines under different oxygen conditions, whereas the sulforhodamine B protein assays perform well under all conditions. However, within a given oxygen environment, both proliferation assays show a correlation with cell number. Therefore, the MTT assay must be used with caution when comparing cell growth or drug response for cells grown in different oxygen environments.Supplementary information: The online version contains supplementary material available at 10.1007/s10616-023-00584-0.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10148801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect assessment of a type of xeno-free and serum-free human adipose-derived mesenchymal stem cells culture medium by proliferation and differentiation capacities. 通过增殖和分化能力评估一种无异种和无血清的人脂肪来源的间充质干细胞培养基的效果。

IF 2 4区生物学

Cytotechnology Pub Date : 2023-10-01 Epub Date: 2023-07-11 DOI: 10.1007/s10616-023-00586-y

Shanshan Chen, Li Meng, Shanshan Wang, Yan Xu, Wenbin Chen, Jianfeng Wei

{"title":"Effect assessment of a type of xeno-free and serum-free human adipose-derived mesenchymal stem cells culture medium by proliferation and differentiation capacities.","authors":"Shanshan Chen, Li Meng, Shanshan Wang, Yan Xu, Wenbin Chen, Jianfeng Wei","doi":"10.1007/s10616-023-00586-y","DOIUrl":"10.1007/s10616-023-00586-y","url":null,"abstract":"Human mesenchymal stem cells (hMSCs) possess broad prospects in pre-clinical research. In vitro amplification of hMSCs requires appropriate medium to reach the number of seed cells with clinical significance. However, the uncertainty of the heterologous components of the traditional fetal bovine serum (FBS) culture medium has great safety risks. Moreover, existing commercial hMSCs medium is very expensive, therefore a safer and more optimal hMSCs medium is urgently needed. Accordingly, we developed five components adipose-derived hMSCs (hADMSCs) medium without xenogenic components, named E5 SFM. which is mainly composed of knockout serum replacement (KSR), and additionally four components such as fibroblast growth factor and transferrin. Here, we mainly compared the E5 SFM with traditional FBS-containing medium and a commercial medium by surface markers testing, proliferation assay as well as osteogenic, adipogenic and chondrogenic differentiation assessment. We demonstrated that hADMSCs cultured in the E5 SFM showed similar morphological characteristics and immunophenotypes to those in other media. Notably, cell proliferative capability was similar to that in the commercial medium, but higher than that in the FBS-containing medium and other media. Additionally, their capabilities of adipogenic and osteogenic differentiation were significantly higher than those of other media. Consequently, we concluded that the E5 SFM medium can not only effectively promote cell proliferation of hMSCs, but also has optimal differentiative capacity and clear and simple ingredients.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10137221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H3K27 acetylation-induced FSTL1 upregulation by P300/RUNX1 co-activation exacerbated autophagy-mediated neuronal damage and NF-κB-stimulated inflammation in Alzheimer's disease. H3K27乙酰化诱导的FSTL1通过P300/RUNX1共激活上调，加剧了阿尔茨海默病中自噬介导的神经元损伤和NF-κB刺激的炎症。

IF 2 4区生物学

Cytotechnology Pub Date : 2023-10-01 Epub Date: 2023-08-04 DOI: 10.1007/s10616-023-00589-9

Dongmei Dai, Junzheng Xie, Yun Zheng, Fangbin Chen, Bin Zhao, Li Miao

引用次数: 0