Cytotechnology最新文献

{"title":"Correction: Protective effects of liquiritin on polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis via miR-206/PI3K/AKT pathway.","authors":"Xuan Cui, Shisan Zhou, Yongtao Lin","doi":"10.1007/s10616-025-00746-2","DOIUrl":"10.1007/s10616-025-00746-2","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1007/s10616-022-00531-5.].</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"97"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZNF384 mediates KRT23 to promote CRC process through the TGF-β/Smad signaling pathway.","authors":"Jianfeng Liu, Yuanyuan Li, Bingling Liao, Qihua Xu, Ying Zhou, Huijun Zhang","doi":"10.1007/s10616-025-00765-z","DOIUrl":"10.1007/s10616-025-00765-z","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a common malignancy of the digestive tract and is the second leading cause of cancer-related death worldwide. Keratin 23 (KRT23), a member of the keratin family, is implicated in the development of various cancers. Hence, this study aimed to clarify the molecular mechanism of KRT23 in the progression of CRC. Quantitative real time polymerase chain reaction (qRT-PCR) and western blot were applied to detected RNA and protein levels. Cell migration, invasion, and apoptosis were measured by wound healing, transwell assay, and flow cytometry, respectively. Glycolysis was reflected by the detection of ATP, lactate production, and glucose consumption. What's more, the binding site of zinc finger protein 384 (ZNF384) and KRT23 was predicted using the relevant website and verified by chromatin immunoprecipitation (CHIP) and dual-luciferase reporter assay. Meanwhile, the related proteins were detected by immunohistochemistry (IHC). A mouse xenograft model was established for in vivo analysis and further verified the role of ZNF384 and KRT23 in CRC. KRT23 was highly expressed in CRC tissues and cells. Functionally, silencing KRT23 inhibited CRC migration and invasion, promoted apoptosis, and impeded epithelial-mesenchymal transition (EMT) and glycolysis process by the TGF-β/Smad signaling pathway. In terms of mechanism, ZNF384 bound to the KRT23 promoter, and the inhibition caused by ZNF384 interference was reversed with KRT23. In vivo, knocking down ZNF384 inhibited tumor growth. In summary, ZNF384 could promote the malignant progression of CRC by regulating the KRT23-mediated TGF-β/Smad signaling pathway.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00765-z.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"111"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional improvements in β-conglycinin by conjugation with pectin by the Maillard reaction.","authors":"Toshiki Takai, Hikaru Murai, Naoko Zushi, Taku Neagari, Iroha Tanabe, Manami Nishiyama, Tadashi Yoshida, Makoto Hattori","doi":"10.1007/s10616-025-00781-z","DOIUrl":"10.1007/s10616-025-00781-z","url":null,"abstract":"<p><p>β-Conglycinin was conjugated with pectin by the Maillard reaction to improve its emulsifying property and reduce its allergenicity. Low methoxy pectin (LMP) and high methoxy pectin (HMP) were conjugated with β-conglycinin. Reaction ratio of β-conglycinin and pectin was 1.0: 5.0 (weight ratio). The Maillard Reaction was conducted at 65 °C at 79% relative humidity for 5 days to prepare the β-conglycinin-LMP conjugate and 7 days to prepare the β-conglycinin-HMP conjugate. The β-conglycinin-pectin conjugates were purified by salting out with ammonium sulfate. Conjugation was confirmed by SDS-PAGE. The results of bicinchoninic acid (BCA) method and phenol sulfuric acid method showed that weight ratio of protein and saccharide of the β-conglycinin-LMP and β-conglycinin-HMP conjugates was 1:1.33 and 1:0.73 respectively. The result of <i>o</i>-phthalaldehyde (OPA) method indicated that free amino groups of β-conglycinin were shielded by conjugation with pectin and the degree of shielding was pronounced especially for the β-conglycinin-HMP conjugate. Solubility of β-conglycinin at pH 5.0 was improved by conjugation with LMP. Emulsifying property of β-conglycinin at pH 5.0 and pH 7.0 and in the presence of 0.2 M NaCl was improved by conjugation with pectin, especially with LMP. Antigenicity and immunogenicity of β-conglycinin were reduced by conjugation with HMP.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"118"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Picroside II regulates the YY1/TGFβ1 axis by inhibiting osteoblast ferroptosis to alleviate symptoms of postmenopausal osteoporosis.","authors":"Haoyu Wang, Jun Qian","doi":"10.1007/s10616-025-00783-x","DOIUrl":"10.1007/s10616-025-00783-x","url":null,"abstract":"<p><p>Picroside II (Pic II) has been used to treat many skeletal diseases. Postmenopausal osteoporosis (PMOP) is a serious skeletal disease that significantly threatens the health of postmenopausal women. We aimed to explore the roles and mechanisms of Pic II in PMOP. PMOP models were established by performing bilateral ovariectomy in rats and stimulating osteoblasts with H₂O₂. In vivo, micro-CT imaging, HE and MASSON staining were performed, and E₂, Ca/Cre, ALP, BGP, SOD, MDA, Fe²⁺ and ROS levels were determined. In vitro, cell viability, apoptosis, mineralization, GSH and Fe²⁺ levels were measured. Both animal and cell experiments detected the expressions of YY1, TGFβ1, SLC7A11, GPX4, RunX2 and BMP2 proteins. Moreover, PMOP cells were treated with N-Acetyl-L-cysteine (a ferroptosis inhibitor) to further explore the mechanisms of Pic II on PMOP. Pic II attenuated bone loss, improved femur pathological injury and increased collagen volume fraction for PMOP rats<i>.</i> Furthermore, after Pic II treatment, PMOP rats exhibited decreased Ca/Cre, ALP, MDA, Fe²⁺ and ROS levels, but increased E₂, BGP and SOD levels. In vitro, Pic II intervention elevated cell viability, GSH level and mineralization, suppressed apoptosis, and reduced ROS and Fe²⁺ levels. Moreover, both animal and cell experiments observed that Pic II upregulated YY1, GPX4, SLC7A11, Runx2 and BMP2 expressions, but downregulated TGFβ1 expression. Importantly, Pic II exhibited similar effects to N-Acetyl-L-cysteine in PMOP cells. Pic II may regulate YY1/TGFβ1 axis by inhibiting osteoblast ferroptosis to alleviate PMOP, suggesting that Pic II may be a novel agent for PMOP treatment.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"114"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for dissociation of oral cancer cell spheroids generated by magnetic 3D bioprinting.","authors":"Natalie Aparecida Rodrigues Fernandes, Camyla Rodrigues Nascimento, Ricardo D Coletta, Carlos Rossa Junior","doi":"10.1007/s10616-025-00776-w","DOIUrl":"10.1007/s10616-025-00776-w","url":null,"abstract":"<p><p>Spheroid 3D cultures yield a better representation of diverse cellular events in comparison with 2D cultures. However, the ability to investigate some specific phenotype/cell biology-related outcomes of experimental interventions by flow cytometry is somewhat limited in spheroid cultures, as it requires a single cell suspension with good preservation of membrane-bound proteins. In this study, we describe a method combining enzymatic and mechanical dissociation of large spheroids of five different oral squamous cell carcinoma (OSCC) cell lines (H314, SCC9, SCC25, UM-SCC1 and HSC3). Spheroids were generated using magnetic 3D bioprinting. Efficacy of dissociation and viability after dissociation were evaluated by flow cytometry. All spheroids were successfully dissociated into single cells with low cell aggregation using this method, except for spheroids of HSC3 cells. H314 and SCC9 spheroids yielded the highest number of events per microliter and therefore also the largest total number of events. H314 and SCC25 spheroids presented the better results for cell viability after dissociation. H314 spheroids also gave the largest total number of viable cell events. The dissociation method described can be useful for researchers interested in flow cytometry-based analysis of spheroid cultures.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00776-w.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"115"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanism of <i>Epimedium</i> in treating diabetic nephropathy based on network pharmacology and experimental validation study.","authors":"Leyu Huang, Hui Li, Ying Han","doi":"10.1007/s10616-025-00748-0","DOIUrl":"10.1007/s10616-025-00748-0","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) is a severe complication of diabetes, characterized by chronic inflammation, metabolic disturbances, and progressive renal damage. Natural perennial herb, such as <i>Epimedium</i>, has shown potential therapeutic effects on DN, but its underlying mechanisms remain unclear. This study aimed to explore the pharmacological mechanisms of <i>Epimedium</i> in the treatment of DN through network pharmacology, molecular docking, and experimental validation. Active components of <i>Epimedium</i> were identified using TCMSP and SwissTargetPrediction databases, while DN-related targets were retrieved from GeneCards, DisGeNET, OMIM, and TTD databases. Overlapping targets were analyzed via PPI network and Cytoscape's cytoHubba plugin to identify hub genes. GO and KEGG enrichment analyses were conducted to explore functional pathways. Molecular docking validated the binding affinity between key targets and active components. Finally, high-glucose-induced HK-2 cell injury models were used to verify the protective effects of <i>Epimedium</i> through RT-qPCR, western blotting, and mitochondrial function assays. A total of 224 overlapping targets were identified, with <i>AKT1, TNF, HSP90AA1</i>, and <i>SRC</i> serving as key hub genes. GO and KEGG analyses revealed significant enrichment in pathways such as the PI3K-Akt signaling pathway and lipid metabolism. Molecular docking demonstrated strong interactions between <i>Epimedium</i> components and hub targets. Experimental validation showed that <i>Epimedium</i> restored nephrin and WT1 protein levels, mitigated mitochondrial dysfunction, and reversed high-glucose-induced overexpression of key targets. <i>Epimedium</i> exerts therapeutic effects on DN through multi-target interactions, primarily via the PI3K-Akt pathway, highlighting its potential as a novel treatment for DN.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00748-0.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"82"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology prediction and experiment validation to discover the mechanism of <i>Sophora japonica</i> Linn. against liver cancer.","authors":"Yahui Ren, Yun Liang, Tao Zhu, Yanru Fan, Sijin Zhang, Mengmeng Zheng, Xiaoxue Xiao, Qingmin Cheng, Yue Liu, Hui Chen, Wei Song","doi":"10.1007/s10616-025-00766-y","DOIUrl":"10.1007/s10616-025-00766-y","url":null,"abstract":"<p><p><i>Sophora japonica</i> Linn., a medicinal and food-homologous plant, is commonly used to resist bacterial, inflammatory, tumor, and other effects. This study aimed to elucidate the multi-target mechanism of action of <i>Sophora japonica</i> Linn. on liver cancer through network pharmacological analysis and verify its effect through biological experiments. The network pharmacology and molecular docking results showed that there were 152 interactivity targets between <i>Sophora japonica</i> Linn. and liver cancer, which were mainly enriched in various biological processes through the AKT and MAPK signaling pathways. In vitro biological experiments showed that isorhamnetin and quercetin, the main active components of <i>Sophora japonica</i> Linn., had significant inhibitory effect on liver cancer cells HepG2. In addition, the expression of AKT and MEK proteins was downregulated, which proved that both isorhamnetin and quercetin promoted apoptosis by activating the AKT and MAPK signaling pathways. In summary, our findings clarify the inhibitory effect of the active ingredients of <i>Sophora japonica</i> Linn. against HepG2 cells and provide inspiration for its clinical application in the treatment of liver cancer.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00766-y.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"101"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IGFBP7 inhibition relieves LPS induced nucleus pulposus cell injury by regulating the ERK1/2 pathway.","authors":"Jun Gao, Chunsheng Gao, Xiaowei Wang, Maoqing Wu","doi":"10.1007/s10616-025-00784-w","DOIUrl":"10.1007/s10616-025-00784-w","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IDD) and its secondary morbidity cause a severe reduction in quality of life. NP cells play a key role in the development of IDD. IGFBP7 plays a role in numerous diseases, especially in orthopedic disorders, but its role and mechanism for IDD are not clear. The aim of this study was to investigate the role and mechanism of IGFBP7 in a cellular model of LPS-induced IDD. Human primary NP cells were cultured, and subsequently, LPS-stimulated human NP cells were employed to establish an IDD cell model. RT-qPCR and western blot assay were conducted to assess the expression of IGFBP7 in NP cells. A small interfering RNA targeting IGFBP7 (IGFBP7-siRNA) was used to explore the role of IGFBP7 in LPS-treated human NP cells. The viability and apoptosis levels of these cells were then evaluated using MTT assay and flow cytometry analysis. The secretion of TNF-α, IL-6, and IL-1β was calculated by ELISA. RT-qPCR and western blot assay were also utilized to measure the expression levels of Bax, Bcl-2, aggrecan, and type II collagen. Furthermore, western blot analysis was employed to detect the phosphorylation levels of ERK1/2 proteins. To explore the role of the ERK1/2 pathway in NP cells, LM22B-10, an ERK activator, was applied. IGFBP7 expression was significantly elevated in LPS-stimulated human NP cells to approximately 3 folds of control levels. LPS significantly inhibited the viability of human NP cells and promoted the level of apoptosis and inflammatory factor secretion. LPS markedly induced the expression of Bax in human NP cells, while suppressed the abundance of Bcl-2, aggrecan, and collagen type II. LPS significantly activated the ERK1/2 pathway in human NP cells, promoting an increase in ERK1/2 phosphorylation levels. All of these phenomena were reversed by IGFBP7-siRNA. LM22B-10 could significantly reverse the effects of IGFBP7-siRNA on LPS-induced human NP cells. Silencing of IGFBP7 could mitigate apoptosis and inflammatory response triggered by LPS in human NP cells by suppressing the ERK1/2 pathway, suggesting a protective role in IDD. IGFBP7 is a potential therapeutic target for IDD.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"116"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study the impact of <i>Galangin</i> and <i>Alpinia galanga L</i>. on the activation of apoptosis in breast cancer.","authors":"Farinaz Malakotikhah, Kahin Shahanipour, Ramesh Monajemi, Ali Mohammad Ahadi, Ali Asghar Rastegari","doi":"10.1007/s10616-025-00789-5","DOIUrl":"10.1007/s10616-025-00789-5","url":null,"abstract":"<p><p>Breast cancer is still a major worldwide health problem. Metastasis, the dissemination of cancerous cells to distant tissues, significantly contributes to death. <i>Galangin</i>, a flavonoid from many plants, has shown encouraging anti-cancer effects in preclinical research. This research sought to examine the impact of <i>Galangin</i> on the transcription of critical invasiveness genes in breast cancer cells. <i>Alpinia galanga L.</i> was extracted, and the solubility of <i>Galangin</i> in excipients was assessed. MCF7 cells were punctured, and the cytotoxicity of <i>Alpinia galanga L.</i> and <i>Galangin</i> was assessed using the MTT assay. The induction of apoptosis was examined by flow cytometry. The transcription of apoptosis-related genes was assessed using quantitative real-time PCR. The assessment of Total Antioxidant Capacity (TAC) and the bioinformatics-based construction of gene networks related to breast cancer were conducted. The study found that <i>Galangin</i> significantly increased the survival rates of MCF7 cells after 72 h of exposure to 1000 µg/ml and 500 µg/ml extracts. The IC50 was set at 500 µg/ml. <i>Galangin</i> treatment led to a 10.4% early apoptosis incidence, 22.2% late apoptosis, 2.39% necrosis, and a 65% survival rate. <i>Alpinia galanga L.</i> extract inducing apoptosis was 19.03%, while <i>Galangin</i> application produced 32.6% apoptosis. The transcription levels of <i>GPX</i> and <i>P53</i> genes associated with programmed cell death were significantly elevated after treatment with <i>Galangin</i>. The transcription level of the <i>MMP2</i> gene was significantly decreased in MCF7 cells treated with <i>Galangin</i> and <i>Alpinia galanga L.</i> compared to the PBS-treated group. Treatment with <i>Galangin</i> and <i>Alpinia galanga L.</i> decreased the total antioxidant capacity. <i>Galangin</i> significantly reduced the antioxidant activities compared to other groups. <i>Galangin</i> shows potential as an effective treatment against breast cancer by regulating cellular growth and death. Further research is required to validate the therapeutic efficacy of <i>Galangin</i> and to clarify its processes in breast cancer.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00789-5.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"119"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different cell culture media on the production and glycosylation of a monoclonal antibody from a CHO cell line.","authors":"Jaeweon Lee, Uriel Ortega-Rodriguez, Chikkathur N Madhavarao, Tongzhong Ju, Thomas O'Connor, Muhammad Ashraf, Seongkyu Yoon","doi":"10.1007/s10616-025-00733-7","DOIUrl":"10.1007/s10616-025-00733-7","url":null,"abstract":"<p><p>Recombinant monoclonal antibodies (mAbs) are commonly produced using Chinese hamster ovary (CHO) cells and the cell culture medium used in bioreactors influences the yield and quality attributes of the protein drug products. The COVID 19 pandemic revealed a vulnerability in the supply chain for necessary reagents (such as culture medium and raw material) for maintaining un-interrupted production of protein drugs with consistent quality. The supply interruption for the cell culture medium ActiPro™ optimized for producing VRC01, an IgG1-κ mAb, from a CHO-K1 cell line, necessitated the search for alternate media. VRC01 mAb is highly glycosylated and can broadly neutralize several strains of Human Immunodeficiency Virus (HIV). We investigated to see if an alternate medium can be used in the production without impacting quality attributes like glycosylation. In our strategy, we used 3 different commercially available media, performed two sets of experiments-with and without media supplements, Cell boost 7a and Cell boost 7b. Cell growth, volumetric production of the mAb protein and glycosylation pattern were compared to identify an alternative medium. Among the tested media based on cell growth, mAb production potential and glycosylation analysis, ActiCHO™ P was found to be a better alternate medium to ActiPro™ medium than EX-CELL® 325 PF CHO medium to produce VRC01 mAb. Overall, the approach used here to establish the impact of variation in medium on protein therapeutic attributes may be used during product development to build in supply chain resilience in drug manufacturing.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00733-7.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"81"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}