Cytotechnology最新文献_第8页

Nrf2 activator Diethyl Maleate attenuates ROS mediated NLRP3 inflammasome activation in murine microglia Nrf2激活剂马来酸二乙酯可减弱小鼠小胶质细胞中ROS介导的NLRP3炎性体激活

IF 2.2 4区生物学

Cytotechnology Pub Date : 2023-11-28 DOI: 10.1007/s10616-023-00609-8

Cagla Kiser, Ceren Perihan Gonul, Sermin Genc

{"title":"Nrf2 activator Diethyl Maleate attenuates ROS mediated NLRP3 inflammasome activation in murine microglia","authors":"Cagla Kiser, Ceren Perihan Gonul, Sermin Genc","doi":"10.1007/s10616-023-00609-8","DOIUrl":"https://doi.org/10.1007/s10616-023-00609-8","url":null,"abstract":"Microglia are the tissue-resident immune cells of the central nervous system. As a part of the innate immune response, NLR Family Pyrin Domain Containing Protein 3 (NLRP3) inflammasome activation leads to cleavage of caspase-1 and triggers secretion of proinflammatory cytokines and may also result in pyroptotic cell death. Inflammasome activation plays a crucial role in inflammatory conditions; aberrant activation of inflammasome contributes to the pathogenesis of neurodegenerative diseases. Diethyl Maleate (DEM) is a promising antiinflammatory chemical to alleviate inflammasome activation. In this study, NLRP3 inflammasome was activated in N9 murine microglia via 1 µg/ml LPS (Lipopolysaccharide) for 4 h and 5 mM ATP (Adenosine 5′-triphosphate) for 1 h, respectively. We demonstrated that 1 h pretreatment of DEM attenuated NLRP3 inflammasome activation in microglial cells. Besides, mitochondrial ROS decreased upon DEM pretreatment in inflammasome-induced cells. Likewise, it ameliorated pyroptotic cell death in microglia. DEM is a potent activator of Nrf2 transcription factor, the key regulator of the antioxidant response pathway. Nrf2 has been a significant target to decrease aberrant inflammasome activation through the antioxidant compounds, including DEM. Here, we have shown that DEM increased Nrf2 translocation to the nucleus, resulting in Nrf2 target gene expression in microglia. In conclusion, DEM is a promising protective agent against NLRP3 inflammasome activation.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"8 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138530450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fish cell line: depositories, web resources and future applications 鱼类细胞系:储存库、网络资源及未来应用

IF 2.2 4区生物学

Cytotechnology Pub Date : 2023-11-27 DOI: 10.1007/s10616-023-00601-2

Murali S. Kumar, Vijay Kumar Singh, Akhilesh Kumar Mishra, Basdeo Kushwaha, Ravindra Kumar, Kuldeep Kumar Lal

{"title":"Fish cell line: depositories, web resources and future applications","authors":"Murali S. Kumar, Vijay Kumar Singh, Akhilesh Kumar Mishra, Basdeo Kushwaha, Ravindra Kumar, Kuldeep Kumar Lal","doi":"10.1007/s10616-023-00601-2","DOIUrl":"https://doi.org/10.1007/s10616-023-00601-2","url":null,"abstract":"Cell lines are important bioresources to study the key biological processes in the areas like virology, pathology, immunology, toxicology, biotechnology, endocrinology and developmental biology. Cell lines developed from fish organs are utilized as a model in vitro system in disease surveillance programs, pharmacology, drug screening and resolving cases of metabolic abnormalities. During last decade, there were consistent efforts made globally to develop new fish cell lines from different organs like brain, eye muscles, fin, gill, heart, kidney, liver, skin, spleen, swim bladder, testes, vertebra etc. This increased use and development of cell lines necessitated the establishment of cell line depositories to store/preserve them and assure their availability to the researchers. These depositories are a source of authenticated and characterized cell lines with set protocols for material transfer agreements, maintenance and shipping as well as logistics enabling cellular research. Hence, it is important to cryopreserve and maintain cell lines in depositories and make them available to the research community. The present article reviews the current status of the fish cell lines available in different depositories across the world, along with the prominent role of cell lines in conservation of life on land or below water.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"3 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138542127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the PrimeFlow RNA assay as a method of detection of SARS-CoV-2 single and dual Infections PrimeFlow RNA检测作为SARS-CoV-2单次和双次感染检测方法的评价

IF 2.2 4区生物学

Cytotechnology Pub Date : 2023-11-24 DOI: 10.1007/s10616-023-00608-9

Shollie M. Falkenberg, Alexa Buckley, Paola Boggiatto

{"title":"Evaluation of the PrimeFlow RNA assay as a method of detection of SARS-CoV-2 single and dual Infections","authors":"Shollie M. Falkenberg, Alexa Buckley, Paola Boggiatto","doi":"10.1007/s10616-023-00608-9","DOIUrl":"https://doi.org/10.1007/s10616-023-00608-9","url":null,"abstract":"Given the implications of increased transmissibility, virulence, host range, and immune escapes of emerging variants of SARS-CoV-2, developing in vitro models that allow for detection of variants and differences in infection dynamics is important. The objective of this study, was to evaluate the PrimeFlow RNA in-situ assay as a method of detection for multiple strains of SARS-CoV-2. Evaluation of detection and infection statuses included single infections with an Alpha, Delta, or Omicron variants and dual infections with Alpha/Omicron or Delta/Omicron. RNA probes specific for the Spike protein coding region, were designed (omicron or non-omicron specific). SARS-CoV-2 RNA was detected in greater frequency in the Vero E6 and minimally in the fetal deer testicle cell lines by flow cytometry using this approach for viral detection of multiple variants. Most evident in the Vero E6 cells, 24 h post infection both Alpha and Delta predominated over Omicron in dual infections. This is the first report using the PrimeFlow assay for the detection of SARS-CoV-2 at the single-cell level and as a potential model for competition of variants utilizing infection dynamics in cell culture.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"31 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138530449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of wasp (Vespa crabro) nest extracts on virus replication of Autographa californica nuclear polyhedrosis virus on Spodoptera frugiperda cell culture 小黄蜂巢提取物对加州自签名核多角体病毒在狐尾蛾细胞培养中复制的影响

IF 2.2 4区生物学

Cytotechnology Pub Date : 2023-11-24 DOI: 10.1007/s10616-023-00603-0

Alev Çaldaş, Ceren Börçek Kasurka, Ömer Ertürk

{"title":"Effects of wasp (Vespa crabro) nest extracts on virus replication of Autographa californica nuclear polyhedrosis virus on Spodoptera frugiperda cell culture","authors":"Alev Çaldaş, Ceren Börçek Kasurka, Ömer Ertürk","doi":"10.1007/s10616-023-00603-0","DOIUrl":"https://doi.org/10.1007/s10616-023-00603-0","url":null,"abstract":"The antiviral properties of the extracts of Vespa crabro nests collected from the Black Sea, Turkey have been investigated on Spodoptera frugiperda (Sf) cell cultures of Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV). The effect of nests on cell viability and cytotoxicity analysis and the antiviral assay was studied, and the cytopathic effects of the virus were detected. The nest's viral content was identified. The impact of nest extracts on the protein synthesis of the virus was investigated. Also interaction with pUC18 plasmid DNA was investigated, to analyse the protective effects of the Vespa crabro nest extract againist to hydroxyl radical-mediated DNA damage. 50 µg/ml concentration of ethanol, acetone, and petroleum ether extracts of the nests reduced the cytopathic effects of baculovirus on Sf cells. The extracts delayed infection above 25 µg/ml concentration. When the effects of nest extracts on virus titer were evaluated; the 50 µg/ml concentration of the acetone extract of the nest showed the highest effect (75%) reducing the virus titer. 25 µg/ml concentration of the ethanol extract of the nest showed the lowest effect (33.33%) with a reduction. The presence of polyhedrin protein was observed at 25 µg/ml concentrations of acetone and petroleum ether extracts. When the potential of the nest extracts to repair DNA damage, the nest extracts were found to have a concentration-dependent repair feature in different applications. As a result of bioactive component analysis, (Z) 9-Tricosane and (cis)-2-nonadecene (1.65%) were found to have the highest % areas. In other respects, 1H-Purine-6-amine, 2-dodecanol and hexadecanoic acid compaunds were Additionally, 1H-Purine-6-amine, 2-dodecanol and hexadecanoic acid compounds, which are associated with antiviral activity, also determined in the biocomponent analysis.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"26 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138542145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulsed electrical stimulation and amino acid derivatives promote collagen gene expression in human dermal fibroblasts 脉冲电刺激和氨基酸衍生物促进人真皮成纤维细胞胶原基因表达

4区生物学

Cytotechnology Pub Date : 2023-11-09 DOI: 10.1007/s10616-023-00604-z

Hiroya Urabe, Ryuji Akimoto, Shohei Kamiya, Katsu Hosoki, Hideyuki Ichikawa, Toshio Nishiyama

引用次数: 0

Nitric oxide-cyclic GMP role in Ang II induced hyperpolarization in bovine aortic endothelium cell line (BAE-1) 一氧化氮-环GMP在angii诱导的牛主动脉内皮细胞系(ae -1)超极化中的作用

4区生物学

Cytotechnology Pub Date : 2023-11-02 DOI: 10.1007/s10616-023-00602-1

Chinar M. Mohammed, Omar A. M. Al-Habib

引用次数: 0

Study on the mechanism of CXCL12/CXCR4-axis-mediated upregulation of IL-8 and IL-6 on the biological function of acute T lymphocyte leukaemia cells CXCL12/ cxcr4轴介导的IL-8、IL-6上调对急性T淋巴细胞白血病细胞生物学功能的影响机制研究

4区生物学

Cytotechnology Pub Date : 2023-10-28 DOI: 10.1007/s10616-023-00600-3

Liping Zhou, Hui Zhao, Chao Zhang, Zhe Chen, Dong Li, Guanglei Qian

引用次数: 0

Construction of a novel kinetic model for the production process of a CVA6 VLP vaccine in CHO cells CHO细胞CVA6 VLP疫苗生产过程新动力学模型的建立

4区生物学

Cytotechnology Pub Date : 2023-10-27 DOI: 10.1007/s10616-023-00598-8

Zhou Xing, Thao Bich Nguyen, Guirong Kanai-Bai, Noriko Yamano-Adachi, Takeshi Omasa

{"title":"Construction of a novel kinetic model for the production process of a CVA6 VLP vaccine in CHO cells","authors":"Zhou Xing, Thao Bich Nguyen, Guirong Kanai-Bai, Noriko Yamano-Adachi, Takeshi Omasa","doi":"10.1007/s10616-023-00598-8","DOIUrl":"https://doi.org/10.1007/s10616-023-00598-8","url":null,"abstract":"Abstract Bioprocess development benefits from kinetic models in many aspects, including scale-up, optimization, and process understanding. However, current models are unable to simulate the production process of a coxsackievirus A6 (CVA6) virus-like particle (VLP) vaccine using Chinese hamster ovary cell culture. In this study, a novel kinetic model was constructed, correlating (1) cell growth, death, and lysis kinetics, (2) metabolism of major metabolites, and (3) CVA6 VLP production. To construct the model, two batches of a laboratory-scale 2 L bioreactor cell culture were prepared and various pH shift strategies were applied to examine the effect of pH shift. The proposed model described the experimental data under various conditions with high accuracy and quantified the effect of pH shift. Next, cell culture performance with various pH shift timings was predicted by the calibrated model. A trade-off relationship was found between product yield and quality. Consequently, multiple objective optimization was performed by integrating desirability methodology with model simulation. Finally, the optimal operating conditions that balanced product yield and quality were predicted. In general, the proposed model improved the process understanding and enabled in silico process development of a CVA6 VLP vaccine.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136262608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosome-mediated circGMPS facilitates the development of gastric cancer cells through miR-144-3p/PUM1 外泌体介导的circGMPS通过miR-144-3p/PUM1促进胃癌细胞的发育

4区生物学

Cytotechnology Pub Date : 2023-10-20 DOI: 10.1007/s10616-023-00597-9

Yuexin Zhang, Wenrui Xie, Wenhong Zheng, Xiaoying Qian, Chengwei Deng

引用次数: 0

Reconstruction of ovarian follicular-like structure by recellularization of a cell-free human ovarian scaffold with mouse fetal ovarian cells 用小鼠胚胎卵巢细胞重建无细胞人卵巢支架重建卵巢卵泡样结构

4区生物学

Cytotechnology Pub Date : 2023-10-06 DOI: 10.1007/s10616-023-00595-x

Maryam Nezhad Sistani, Saeed Zavareh, Mojtaba Rezazadeh Valojerdi, Mojdeh Salehnia

引用次数: 0