Current Medical Mycology最新文献

{"title":"Detection of fungal and bacterial contamination of hazelnut and determination of aflatoxin B by HPLC method in Isfahan, Iran.","authors":"Elham Saffari,&nbsp;Mahboobeh Madani,&nbsp;Vajihe Karbasizade,&nbsp;Pegah Shakib","doi":"10.18502/cmm.7.4.8404","DOIUrl":"https://doi.org/10.18502/cmm.7.4.8404","url":null,"abstract":"<p><strong>Background and purpose: </strong>Due to the fact that fungal species, such as <i>Aspergillus flavus</i> and <i>Aspergillus parasiticus</i> produce carcinogenic and mutagenic aflatoxins and have the potential to produce fungal secondary metabolites, fungal contamination should be avoided. This study was conducted using the HPLC method and aimed to examine the fungal contamination of Isfahan hazelnuts in order to identify the presence of Aflatoxins.</p><p><strong>Materials and methods: </strong>In total, 100 samples of hazelnuts were randomly collected from supermarkets in Isfahan. The samples were then cultured on Sabouraud dextrose agar media and analyzed to determine fungal contaminations. The aflatoxin analysis was carried out using the HPLC method.</p><p><strong>Results: </strong>It was discovered that nine genera of fungi, namely <i>Aspergillus</i>, <i>Penicillium</i>, <i>Rhizopus</i>, <i>Ulocladium</i>, <i>Alternaria</i>, <i>Drechselera</i>, <i>Trichothecium</i>, <i>Scopulariopsis</i>, and <i>Mucor</i> were identified in 78% of the samples. Samples contaminated with <i>Aspergillus flavus</i> (22 samples) were studied to determine the presence of aflatoxin. The results showed that 16 (72.72%) of the samples were contaminated with AFB1, AFB2, and AFG2 and the mean concentrations were 0.926, 0.563, and 0.155 ng/g, respectively.</p><p><strong>Conclusion: </strong>Some parameters that affect mycotoxin production are temperature, food substrate, the strain of the mold, and other environmental factors. Due to the toxigenic quality of some of these fungi and their hazard to human health, it is crucial that fungal contamination and aflatoxin identification tests are carried out before certain products are made available to the mass market.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":" ","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40395144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute pulmonary histoplasmosis related to occupational roofing: A case report of two brothers.","authors":"Martin Gnoni,&nbsp;Timothy McCann,&nbsp;Adrian Riva-Moscoso,&nbsp;Fortunato S Príncipe-Meneses,&nbsp;Diego Chambergo-Michilot","doi":"10.18502/cmm.7.4.8409","DOIUrl":"https://doi.org/10.18502/cmm.7.4.8409","url":null,"abstract":"<p><strong>Background and purpose: </strong><i>Histoplasma capsulatum</i> is the cause of a prevalent fungal disease in certain regions in the United States of America, like Ohio and the Mississippi River. Its clinical manifestations range from asymptomatic to life-threatening diseases, according to the immune system. A definitive diagnosis is made by biopsy.</p><p><strong>Case report: </strong>Two middle-aged brothers presented with a nine-day history of severe progressive dyspnea. Both were living in Cincinnati, Ohio, and encountered bird droppings 7 days prior to symptoms while working on a roofing project. It should be mentioned that they were not wearing masks. After extensive testing, they were diagnosed with acute pulmonary histoplasmosis. Both were successfully treated with azole-derivative fungal therapy.</p><p><strong>Conclusion: </strong>This is the first case of histoplasmosis acquired through occupational exposure related to roofing and is unique given the two patients were siblings.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":" ","pages":"34-37"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40395149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology, risk factors, species distribution, and antifungal susceptibility of candidemia among hospitalized patients with COVID-19.","authors":"Hasti Kamali Sarvestani,&nbsp;Shahram Mahmoudi,&nbsp;Pegah Afarinesh Khaki,&nbsp;Saham Ansari,&nbsp;Sara Ghaderkhani,&nbsp;Davoud Roostaei,&nbsp;Roshanak Daei Ghazvini,&nbsp;Seyed Jamal Hashemi,&nbsp;Zahra Rafat,&nbsp;Alireza Abollahi","doi":"10.18502/cmm.7.4.8406","DOIUrl":"https://doi.org/10.18502/cmm.7.4.8406","url":null,"abstract":"<p><strong>Background and purpose: </strong>The pandemic of COVID-19 has caused a worldwide health crisis. Candidemia is a potentially lethal condition that has not yet been enough discussed in patients with COVID-19. The current study aimed to investigate the prevalence of candidemia among Iranian COVID-19 patients and characterize its causative agents and the antifungal susceptibility pattern.</p><p><strong>Materials and methods: </strong>The present cross-sectional survey was carried out from March 2020 to March 2021 at Imam Khomeini Hospital, Tehran, Iran. Blood specimens were obtained from patients with confirmed coronavirus infection who also had criteria for candidemia and were examined for any <i>Candida</i> species by conventional and molecular techniques. Susceptibility of isolates to amphotericin B, voriconazole, itraconazole, fluconazole, caspofungin, and 5-flucytosine was tested using the CLSI broth dilution technique.</p><p><strong>Results: </strong>In total, 153 patients with COVID-19 were included and candidemia was confirmed in 12 (7.8 %) of them. The majority of patients were ≥ 50 years of age (n=9) and female (n=8). Moreover, 6 out of the 12 patients were diabetic. The presence of central venous catheters, broad-spectrum antibiotic therapy, ICU admission, and mechanical ventilation was observed in all patients. The <i>C. albicans</i> (n=7, 58.3 %) and <i>C. dubliniensis</i> (n=2, 16.7%) were the most common isolated species. Amphotericin B and 5-flucytosine were the most active drugs. Despite antifungal treatment, 4 out of 12 patients (33.3 %) died.</p><p><strong>Conclusion: </strong>Due to the high mortality, the early diagnosis and proper treatment of candidemia are essential requirements for optimal clinical outcomes in COVID-19 patients.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":" ","pages":"12-18"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40395148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal pulmonary <i>Scedosporium aurantiacum</i> infection in a patient after near-drowning: A case report.","authors":"Roya Ghasemian,&nbsp;Azadeh Bandegani,&nbsp;Firoozeh Kermani,&nbsp;Leila Faeli,&nbsp;Behrad Roohi,&nbsp;Elham Yousefi-Abdolmaleki,&nbsp;Mohammad T Hedayati,&nbsp;Emmanuel Roilides,&nbsp;Tahereh Shokohi","doi":"10.18502/cmm.7.4.8410","DOIUrl":"https://doi.org/10.18502/cmm.7.4.8410","url":null,"abstract":"<p><strong>Background and purpose: </strong><i>Scedosporium</i> spp. is a saprophytic fungus that may cause invasive pulmonary infection due to the aspiration of contaminated water in both immunosuppressed and immunocompetent hosts.</p><p><strong>Case report: </strong>Herein, we report a fatal case of pulmonary infection caused by <i>Scedosporium</i> species associated with a car crash and near-drowning in a sewage canal. <i>Scedosporium aurantiacum</i> isolated from bronchoalveolar lavage was identified by PCR-sequencing of <i>β-tubulin</i> genes. The minimum inhibitory concentration values for amphotericin B, itraconazole, posaconazole, isavuconazole were >16 µg/ml, and >8 µg/ml for anidulafungin, micafungin, and caspofungin. Voriconazole was found to be the most active agent with a MIC of 1 µg/ml.</p><p><strong>Conclusion: </strong>This report, as the first case of pulmonary scedosporiosis after near-drowning in Iran, highlights the importance of high suspicion in near-drowning victims, prompt identification of <i>Scedosporium</i> spp., and early initiation of appropriate antifungal therapy.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":" ","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40395145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Allium cepa</i> loaded polyacrylonitrile and polyvinyl pyrrolidone nanofibers on <i>Candida albicans</i> growth and the expression of <i>CDR1</i> and <i>CDR2</i> genes.","authors":"Azam Nademi,&nbsp;Masoomeh Shams-Ghahfarokhi,&nbsp;Mehdi Razzaghi-Abyaneh","doi":"10.18502/cmm.7.4.8408","DOIUrl":"https://doi.org/10.18502/cmm.7.4.8408","url":null,"abstract":"<p><strong>Background and purpose: </strong>This study aimed to assess the effect of <i>Allium cepa</i> ethanolic extract (ACE) loaded polyacrylonitrile (PAN) and polyvinyl pyrrolidone (PVP) nanofibers on <i>Candida albicans</i> (<i>C. albicans</i>) <i>CDR1</i> and <i>CDR2</i> genes expression.</p><p><strong>Materials and methods: </strong>The minimum inhibitory concentrations (MICs) of ACE against <i>C. albicans</i> ATCC 10231 and clinical fluconazole (FLC)-resistant <i>C. albicans</i> PFCC 93-902 were determined using the Clinical and Laboratory Standards Institute (CLSI) protocol (M27-Ed4) at a concentration range of 45.3-5800 µg/ml. The nanofibers containing ACE (60 wt%) were fabricated using the electrospinning technique. The expression of the <i>CDR1</i> and <i>CDR2</i> genes was studied in the fungus exposed to ACE-loaded nanofibers and 0.5×MIC concentration of FLC using the real-time polymerase chain reaction.</p><p><strong>Results: </strong>MIC₅₀ and MIC₉₀ of ACE against FLC-resistant <i>C. albicans</i> were 725 and 1450 μg/mL, respectively. The expression of <i>CDR1</i> (4.5-fold) and <i>CDR2</i> (6.3-fold) were down-regulated after the exposure of FLC-resistant <i>C. albicans</i> to ACE-loaded nanofibers (<i>P</i><0.05). Furthermore, the expression of <i>CDR1</i> (2.8-fold) and <i>CDR2</i> (3.2-fold) were up-regulated in FLC-treated <i>C. albicans</i> (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>The results revealed that nanofibers containing ACE interact with drug-resistant genes expressed in <i>C. albicans</i>. Further studies are recommended to investigate the mode of action and other biological activities of ACE-loaded nanofibers against <i>C. albicans</i> and other pathogenic fungi.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":" ","pages":"28-33"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40395146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High incidence of azole resistance among Candida albicans and C. glabrata isolates in Northeastern Iran","authors":"H. Zarrinfar, Z. Kord, A. Fata","doi":"10.18502/cmm.7.3.7801","DOIUrl":"https://doi.org/10.18502/cmm.7.3.7801","url":null,"abstract":"Background and Purpose: Resistance to antifungal drugs is increasing among Candida isolates from patients with vulvovaginal candidiasis (VVC). Lack of correct diagnosis of Candida causing VVC and the experimental use of antifungal drugs are the main causes of this resistance. This study aimed to determine the susceptibility of antifungal drugs against Candida species isolated from VVC in Northeastern Iran. Materials and Methods: Among women suspected of VVC, 189 vaginal discharge specimens were evaluated. Candida isolates detected by polymerase chain reaction-restriction fragment length polymorphism were examined by standard antifungal disk diffusion susceptibility testing method for voriconazole, itraconazole, fluconazole, and ketoconazole. The susceptibility pattern of these antifungals was reported as sensitive, susceptible dose-dependent, and resistant. The results were evaluated by SPSS software and analyzed by Pearson chi-squared test. Results: Among the vaginal specimens, 108 out of 189 Candida isolates were identified as C. albicans, C. glabrata, C. kefyr, C. parapsilosis, and C. tropicalis. The susceptibility rates of Candida isolates to voriconazole, ketoconazole, itraconazole, and fluconazole were 92.6%, 90.7%, 68.5%, and 63.9%, respectively. Moreover, the resistance rates to fluconazole, itraconazole, ketoconazole, and itraconazole were 15.7%, 8.3%, 1.9%, and 1.9%, respectively. The C. glabrata and C. albicans isolates were resistant to antifungal discs among 93% and 20% of the specimens, respectively. Conclusion: The C. glabrata and C. albicans species showed the highest resistance to antifungal drugs. Furthermore, Candida isolates showed the highest sensitivity to voriconazole and ketoconazole and the lowest sensitivity to fluconazole.","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"67 1","pages":"18 - 21"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90763627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition and antifungal activities of aromatic water of Zataria multiflora Boiss.","authors":"Ali Arabi Monfared, Maryam Yazdanpanah, Z. Zareshahrabadi, K. Pakshir, Mehdi Ghahartars, D. Mehrabani, Samira Yazdanpanah, A. Iraji, K. Zomorodian","doi":"10.18502/CMM.7.3.7255","DOIUrl":"https://doi.org/10.18502/CMM.7.3.7255","url":null,"abstract":"Background and Purpose: In Iranian culture, aromatic waters harboring a slight amount of essential oil have been popularly used for many years as a pleasant non-alcoholic drink with various medicinal properties. In this study, chemical composition of Zataria multiflora Boiss. (ZM) aromatic water was determined and its in vitro and in vivo antifungal properties were investigated. Materials and Methods: Chemical composition of the essential oil extracted from aromatic water (AW) of ZM was analyzed by Gas chromatography and gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity of the AW against Candida species was determined by broth micro-dilution methods. Additionally, biofilm formation inhibition and antioxidant activity of the AW were measured using XTT reduction and DPPH methods, respectively. Antifungal activities of the AW in the prevention and/or treatment of gastrointestinal (GI) candidiasis in animal models were also evaluated. Results: The GC-MS analysis revealed that the major constituents of ZM AW were Carvacrol (46.56%) and Thymol (40.67%). The ZM AW inhibited the growth and biofilm formation of Candida species in the range of 0.25-0.5 V/V. Moreover, ZM AW significantly decreased Candida colonization in therapeutic groups (P<0.05). Conclusion: Given the wide therapeutic potential of ZM AW, including antifungal and antioxidant activities, it might be possible to use it in the management of mucocutaneous or alimentary candidiasis.","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"58 1","pages":"29 - 35"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80205637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaginal and oral use of probiotics as adjunctive therapy to fluconazole in patients with vulvovaginal candidiasis: A clinical trial on Iranian women","authors":"Z. Vahedpoor, M. Abastabar, M. Sehat, P. Talebian, Tayebeh Felfelian fini, Zahra Dastanpour, I. Haghani, Ramtin Chelongarian, M. Nazeri","doi":"10.18502/cmm.7.3.7803","DOIUrl":"https://doi.org/10.18502/cmm.7.3.7803","url":null,"abstract":"Background and Purpose: Vulvovaginal candidiasis is considered the second most prevalent gynecologic infection among women and one of the main reasons for referring to a gynecologist. During recent decades, probiotic usage has been defined as one of the therapeutic regimens for vaginal candidiasis management, but these findings were controversial. The current study was conducted to determine the effect of fluconazole plus vaginal and oral probiotics supplementation on clinical and mycological improvement of vaginal candidiasis concomitant with antifungal susceptibility of Candida species to fluconazole. Materials and Methods: This double-blind, randomized, placebo-controlled trial was conducted on 76 women with vaginal candidiasis admitted to Naghavi and Imam Reza Gynecology Clinics in Kashan, Central Iran, from July 2017 to March 2020. Patients were diagnosed according to vaginal candidiasis symptoms and positive culture for Candida species. The patients were divided into two groups; one of them received fluconazole plus vaginal and oral probiotics, while the other one received fluconazole with placebo. The clinical and mycological findings were recorded before and after the treatment. In vitro, the fluconazole susceptibility test was determined by the microdilution method according to the Clinical and Laboratory Standards Institute (M27-A3) for the baseline Candida isolates. Conclusion: Based on the findings, 35 days after the intervention, a significant reduction was reported in vaginal candidiasis symptoms in the probiotics supplementation group. Although probiotics supplementation therapy was a better mycological cure, compared to the fluconazole with the placebo group, this difference was not significant (68.4% vs. 46.9%, P=0.184). Exclusion of resistant and susceptible dose-dependent strain in the regression model demonstrated a significant reduction in positive culture probiotics in the supplementation group. Results: Oral and vaginal supplementation with probiotics for 4 weeks played a significant role in the elimination of vaginal candidiasis symptoms. Adjustment of clinical and mycological responses with drug resistance patterns of patients could open a promising horizon for probiotics consumption as a complementary treatment.","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"39 1","pages":"36 - 43"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90933497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of Malassezia species isolated from neonates hospitalized in Neonatal intensive care units and their mothers","authors":"K. Zomorodian, Maryam Naderibeni, H. Mirhendi, Mostajab Razavi Nejad, S. M. Saneian, M. Mahmoodi, M. Kharazi, H. Khodadadi, K. Pakshir, M. Motamedi","doi":"10.18502/cmm.7.3.7800","DOIUrl":"https://doi.org/10.18502/cmm.7.3.7800","url":null,"abstract":"Background and Purpose: Given the important role of Malassezia spp. in skin diseases and other associated infections in neonates, this study aimed to investigate the presence and frequency of Malassezia spp. in the skin of neonates hospitalized in neonatal intensive care units and their mothers using culture and accurate molecular-based methods. Materials and Methods: In total, 205 samples were collected from 130 neonates (>4-day-old) and 75 mothers. Isolation of Malassezia spp. from the skin was performed using Leeming-Notman agar and modified Dixon agar media. To compare the Malassezia microflora on the skin of the neonates and their mothers, a polymerase chain reaction-sequencing method was performed for spp. identification of 92 isolates obtained from neonates and their mothers. Moreover, possible associated risk factors for the colonization of Malassezia spp. on the skin were recorded. Results: Cultures from 62.3% of neonates and 77.3% of mothers were positive for Malassezia spp. growth. Malassezia globosa was the most prevalent isolated spp. found in the skin of the study population. It is noteworthy that a rare Malassezia spp., Malassezia arunalokei, was isolated from the skin of one neonate. There was a 76% similarity between the mother-neonate isolate sequences results. The statistical analysis showed that the type of feeding is a significant (P<0.001) associated factor for Malassezia skin colonization. Conclusion: The findings support the hypothesis that the colonization of Malassezia in neonates is significantly influenced by that of the mother, and this may be associated with breastfeeding.","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"41 1","pages":"13 - 17"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88479461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of Candida species isolated from candiduria and its risk factors in neonates and children","authors":"Fariba Shirvani, M. Fattahi","doi":"10.18502/cmm.7.3.7799","DOIUrl":"https://doi.org/10.18502/cmm.7.3.7799","url":null,"abstract":"Background and Purpose: The present study was performed to raise attention on the frequency of Candida spp. and evaluation of risk factors of candiduria in neonates and children. Materials and Methods: In total, 60 urine samples were collected from the suspected neonates and children. Identification of Candida at species level was performed using the polymerase chain reaction-restriction fragment length polymorphism approach. Results: The restriction fragment length polymorphism fingerprint analysis revealed that Candida parapsilosis (n=17; 28.33 %) is the most prevalent isolated species followed by Candida albicans (n=9; 15%), Candida tropicalis (n=4; 9.52%), and C. glabrata (n=2; 4.76%). All of the C. albicans and C. parapsilosis complex strains were identified as C. albicans with HWP1 gene primers and using the NlaIII restriction enzyme activity, respectively. In this study, none of the mentioned factors was the cause of infection, but they could be considered risk factors. The mean hospital stay was 21 days (range: 7-21 days). More than 90% of the patients had a urinary catheter, and about 26% of them received antibiotics. Regarding the risk factors, there was no significant difference between the two groups of candidiasis in terms of C. albicans and non-albicans Candida (P<0.01). Conclusion: Candiduria has always been a challenging issue, especially in children admitted to hospitals. Outcome of candiduria in patients with generally healthy is little.","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"42 1","pages":"9 - 12"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88686318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}