Current Issues in Molecular Biology最新文献_第9页

Circulating Nucleosomes and Histones in the Development of Lung Injury and Sepsis. 循环核小体和组蛋白在肺损伤和败血症发展中的作用。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-19 DOI: 10.3390/cimb47020133

Saugata Dutta, Sauradeep Dutta, Payaningal R Somanath, S Priya Narayanan, Xiaoyun Wang, Duo Zhang

{"title":"Circulating Nucleosomes and Histones in the Development of Lung Injury and Sepsis.","authors":"Saugata Dutta, Sauradeep Dutta, Payaningal R Somanath, S Priya Narayanan, Xiaoyun Wang, Duo Zhang","doi":"10.3390/cimb47020133","DOIUrl":"10.3390/cimb47020133","url":null,"abstract":"Cellular nucleosomes-the structural and functional units of chromatin-are inherently present in cells. During cellular damage or cell death, nucleosomes are released into circulation, either actively or passively. Once released, nucleosomes can become immunogenic entities through various mechanisms. The nucleosomal proteins in nucleosomes, called histones, play a pivotal role in inducing immunogenicity. However, intact nucleosomes are more immunogenic than the histones alone, as nucleosomal double-stranded deoxyribonucleic acid (dsDNA) enhances its immunogenic potential. Our recent study has shown that circulating histones are predominantly nucleosomal histones rather than free histones. Consequently, circulating histones primarily function as integral parts of circulating nucleosomes rather than acting independently. Circulating nucleosomes and their associated histones are implicated in the pathogenesis of a wide array of diseases. Notably, they are critical in the pathogenesis of lung injury and sepsis. These diseases have high morbidity and mortality rates and lack early diagnostic biomarkers. Further investigation is required to fully elucidate the role of circulating nucleosomes and their associated histones in disease processes. This review aims to discuss the current understanding of circulating nucleosomes and histones in the pathogenesis of lung injury and sepsis, with a focus on the underlying mechanisms.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ganoderma lucidum Extract Modulates Gene Expression Profiles Associated with Antioxidant Defense, Cytoprotection, and Senescence in Human Dermal Fibroblasts: Investigation of Quantitative Gene Expression by qPCR. 灵芝提取物调节人皮肤成纤维细胞中与抗氧化防御、细胞保护和衰老相关的基因表达谱：qPCR定量基因表达的研究

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-18 DOI: 10.3390/cimb47020130

Harald Kühnel, Markus Seiler, Barbara Feldhofer, Atefeh Ebrahimian, Michael Maurer

{"title":"Ganoderma lucidum Extract Modulates Gene Expression Profiles Associated with Antioxidant Defense, Cytoprotection, and Senescence in Human Dermal Fibroblasts: Investigation of Quantitative Gene Expression by qPCR.","authors":"Harald Kühnel, Markus Seiler, Barbara Feldhofer, Atefeh Ebrahimian, Michael Maurer","doi":"10.3390/cimb47020130","DOIUrl":"10.3390/cimb47020130","url":null,"abstract":"Cellular senescence plays a crucial role in skin aging, with senescent dermal fibroblasts contributing to reduced skin elasticity and increased inflammation. This study investigated the potential of Ganoderma lucidum (Reishi) ethanol extract to modulate the senescent phenotype of human dermal fibroblasts. Reishi powder of two different vendors was used. The extract was produced by extracting the Reishi powder for at least three weeks in 40% ethanol at room temperature. Etoposide-induced senescent fibroblasts were treated with Reishi extracts from two commercial sources for 14 days. Gene expression analysis was performed using qPCR to assess senescence makers, antioxidant defense, and extracellular matrix remodeling. Results showed that Reishi extracts significantly upregulated antioxidant and cytoprotective genes, including Heme oxygenase 1 (HO-1), γ-Glutamylcysteine synthetase (γGCS-L), and NAD(P)H dehydrogenase [quinone] 1 (NQO1), compared to untreated controls. Importantly, Reishi treatment suppressed the expression of p16INK4a, a key marker of cellular senescence, while transiently upregulating p21Cip1. The extracts also demonstrated potential senolytic properties, reducing the percentage of senescent cells as measured by senescence-associated β-galactosidase staining. However, Reishi treatment did not mitigate the upregulation of MMP1 and IL-8 in one Reishi treatment group, indicating differences in the preparations of different vendors. These findings suggest that Ganoderma lucidum extract may help alleviate some aspects of cellular senescence in dermal fibroblasts, primarily through enhanced antioxidant defense and cytoprotection, potentially offering a novel approach to combat skin aging.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Litsea coreana). 山楂茶嫩叶变色的qRT-PCR内参基因选择。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-18 DOI: 10.3390/cimb47020131

Qianli Dai, Min Lu, Ximeng Yang, Chenggong Lei, Feiyi Huang, Xueping Hu, Xin Huang, Xiaolong Nie, Daojing Chen, Sicheng Huang, Hengxing Zhu

{"title":"qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Litsea coreana).","authors":"Qianli Dai, Min Lu, Ximeng Yang, Chenggong Lei, Feiyi Huang, Xueping Hu, Xin Huang, Xiaolong Nie, Daojing Chen, Sicheng Huang, Hengxing Zhu","doi":"10.3390/cimb47020131","DOIUrl":"10.3390/cimb47020131","url":null,"abstract":"To identify stable reference genes for qRT-PCR analysis across different developmental stages and color variations of tender leaves in Litsea coreana, seven candidate reference genes were selected based on existing transcriptome data. qRT-PCR was performed on tender leaves of L. coreana at various stages and under different color conditions. The stability of these genes was evaluated using GeNorm (version 2003), NormFinder (version 0953), BestKeeper (version 2003), and ReFinder software (version 2004). The most stable genes were selected, and the stability of the chosen reference genes was validated. RPL and ACT were the most stable genes across different leaf developmental stages, while ACT and EF1-α showed the highest stability across different leaf colors. Overall, ACT and EF1-α were the most stable reference genes for both developmental stages and color variations. ACT and EF1-α can be used as reliable reference genes for gene expression studies in the color change process of L. coreana tender leaves. This will provide a foundation for further research into the molecular mechanisms of leaf color changes and the development of color regulation genes in L. coreana.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tyrosine Kinase Inhibitor Post-Allogeneic Stem Cell Transplantation in Adult Philadelphia-Positive B-Acute Lymphoblastic Leukemia: State of the Art and Future Directions. 酪氨酸激酶抑制剂异体干细胞移植治疗成人费城阳性b型急性淋巴母细胞白血病：技术现状和未来方向。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-18 DOI: 10.3390/cimb47020129

Martina Canichella, Paolo de Fabritiis

{"title":"Tyrosine Kinase Inhibitor Post-Allogeneic Stem Cell Transplantation in Adult Philadelphia-Positive B-Acute Lymphoblastic Leukemia: State of the Art and Future Directions.","authors":"Martina Canichella, Paolo de Fabritiis","doi":"10.3390/cimb47020129","DOIUrl":"10.3390/cimb47020129","url":null,"abstract":"In a scenario characterized by continuous improvement in outcomes, Philadelphia chromosome-positive (Ph+) ALL, once considered a biologically defined subtype with one of the poorest prognoses, now includes patients achieving long-term survival even without allogeneic stem cell transplantation. First-line therapy is increasingly adopting a chemo-free approach, combining tyrosine kinase inhibitors (TKIs) with immunotherapy-specifically blinatumomab-which has resulted in high rates of complete molecular responses and improved survival outcomes. Within this paradigm shift, the allocation to transplantation is becoming increasingly selective and genomically oriented, focusing on patients with particularly unfavorable prognostic and predictive factors. For patients undergoing transplantation, maintenance therapy with TKIs has emerged as one of the most important strategies to reduce the risk of relapse. However, there remains considerable uncertainty regarding which patients benefit most from this approach, the optimal TKI agents, dosing strategies, and the duration of maintenance therapy. In this review, we aim to consolidate the available evidence on this topic, analyzing it in the context of the most recent clinical experiences.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Levels of Proangiogenic Molecules and Terminal Complement Complex C5b-9 in the Crown of Circulating sEVs in Patients with Recurrent Glioblastomas: Relationship with Tumor Molecular Characteristics. 复发性胶质母细胞瘤患者循环sev冠中促血管生成分子和末端补体复合物C5b-9的水平：与肿瘤分子特征的关系

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-18 DOI: 10.3390/cimb47020132

Natalia Yunusova, Eldar Tulendinov, Dmitry Svarovsky, Anastasia Ryabova, Irina Kondakova, Anastasia Ponomaryova, Sergey Vtorushin, Stanislav Tabakaev, Dmitry Korshunov, Tatiana Shtam, Svetlana Tamkovich, Evgeny Choynzonov

{"title":"Levels of Proangiogenic Molecules and Terminal Complement Complex C5b-9 in the Crown of Circulating sEVs in Patients with Recurrent Glioblastomas: Relationship with Tumor Molecular Characteristics.","authors":"Natalia Yunusova, Eldar Tulendinov, Dmitry Svarovsky, Anastasia Ryabova, Irina Kondakova, Anastasia Ponomaryova, Sergey Vtorushin, Stanislav Tabakaev, Dmitry Korshunov, Tatiana Shtam, Svetlana Tamkovich, Evgeny Choynzonov","doi":"10.3390/cimb47020132","DOIUrl":"10.3390/cimb47020132","url":null,"abstract":"Circulating small extracellular vesicles (sEVs) are emerging as potential biomarkers for glioblastoma progression. This study aimed to compare the levels of matrix metalloproteinases (MMP2 and MMP9), terminal complement complex (C5b-9), and VEGF-A in circulating sEVs in glioblastoma patients (GBMPs) with and without tumor recurrence. Using differential ultracentrifugation, sEVs were isolated from blood samples of GBMPs with no tumor recurrence for over one year (n = 6) and after first relapse (n = 14). The vesicles were characterized and quantified using flow cytometry. In both groups, C5b-9 was predominantly detected on tumor-specific circulating sEVs (glial fibrillary acidic protein (GFAP)-positive sEVs) with high VEGF-A expression, while C5b-9 was significantly less frequent on sEVs with low VEGF-A expression (p < 0.05). GFAP+VEGF+dimMMP2-C5b-9+ vesicles were rarely detected in GBMPs without relapse, suggesting their potential utility as biomarkers for a favorable relapse-free prognosis. In recurrent GBMPs, a positive correlation was observed between GFAP+VEGF+bright MMP2+C5b-9+ sEVs and MGMT gene promoter methylation levels (r = 0.543, p < 0.05). Additionally, a trend toward a negative correlation was found between GFAP+VEGF+bright MMP2+C5b-9- sEVs and mutant p53 expression in primary tumor tissue (r = -0.44, p = 0.114). These findings suggest that sEV profiles may serve as valuable prognostic markers for glioblastoma recurrence and treatment responses.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Areca catechu L. Extract Inhibits Colorectal Cancer Tumor Growth by Modulating Cell Apoptosis and Autophagy. 槟榔提取物通过调节细胞凋亡和自噬抑制结直肠癌肿瘤生长。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-17 DOI: 10.3390/cimb47020128

Meng-Hsiu Tsai, Chang-Han Chen, Chien-Lin Chen, Mei-Hsien Lee, Li-Ching Wu, Yi-Chiung Hsu, Chao-Yang Hsiao, Chang-Ti Lee, Kuo-Li Pi, Li-Jen Su

{"title":"Areca catechu L. Extract Inhibits Colorectal Cancer Tumor Growth by Modulating Cell Apoptosis and Autophagy.","authors":"Meng-Hsiu Tsai, Chang-Han Chen, Chien-Lin Chen, Mei-Hsien Lee, Li-Ching Wu, Yi-Chiung Hsu, Chao-Yang Hsiao, Chang-Ti Lee, Kuo-Li Pi, Li-Jen Su","doi":"10.3390/cimb47020128","DOIUrl":"10.3390/cimb47020128","url":null,"abstract":"Colorectal cancer (CRC) is a common cancer globally, and chemotherapy often causes severe complications, necessitating effective drugs with minimal side effects. As Areca catechu L. extract (ACE) is a Traditional Chinese Medicine that contains numerous active compounds with anticancer effects, in this study, the Cell Counting Kit-8 (CCK-8) assay was used to determine ACE's effect on CRC cell lines, revealing that it significantly inhibits CoLo320DM and HCT116 cells. In vivo experiments with NU-Foxn1nu mice indicated that ACE inhibits tumor growth, while a flow cytometry assay revealed that higher ACE concentrations increased cell apoptosis and ROS levels. Next-generation sequencing (NGS) showed that ACE increases the fold changes in apoptosis, DNA damage, and autophagy-related genes while inhibiting the fold changes in cell proliferation and Wnt signaling pathway genes. We conducted Western blotting to confirm these findings. Overall, ACE demonstrates potential as a drug candidate by promoting apoptosis and autophagy, and significantly reducing cell viability and tumor growth, thus offering a new approach for effective colorectal cancer treatment with minimal side effects.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-451 in Inflammatory Diseases: Molecular Mechanisms, Biomarkers, and Therapeutic Applications-A Comprehensive Review Beyond Oncology. MiR-451在炎性疾病中的作用：分子机制、生物标志物和治疗应用——肿瘤学以外的综合综述

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-16 DOI: 10.3390/cimb47020127

Fei-Xiang Wang, Guo Mu, Zi-Hang Yu, Zhen-Shan Qin, Xing Zhao, Zu-An Shi, Xin Fan, Li Liu, Ye Chen, Jun Zhou

{"title":"MiR-451 in Inflammatory Diseases: Molecular Mechanisms, Biomarkers, and Therapeutic Applications-A Comprehensive Review Beyond Oncology.","authors":"Fei-Xiang Wang, Guo Mu, Zi-Hang Yu, Zhen-Shan Qin, Xing Zhao, Zu-An Shi, Xin Fan, Li Liu, Ye Chen, Jun Zhou","doi":"10.3390/cimb47020127","DOIUrl":"10.3390/cimb47020127","url":null,"abstract":"MicroRNAs play crucial roles in regulating inflammatory responses and disease progression. Since its identification on chromosome 17q11.2 in 2005, miR-451 has emerged as a key regulator of multiple physiological and pathological processes. While its role in cancer has been extensively documented, accumulating evidence reveals miR-451's broader significance in inflammatory conditions through the regulation of NF-κB, AMPK, and PI3K signaling pathways. This comprehensive review systematically analyzes miR-451's multifaceted functions in inflammatory diseases, with particular focus on ischemia-reperfusion injury, arthritis, and acute organ injuries. We present compelling evidence for miR-451's potential as a diagnostic biomarker, demonstrating its distinctive expression patterns across various biological specimens and disease states. Furthermore, we elucidate how miR-451 modulates inflammatory responses through the regulation of immune cell populations, including microglia activation, macrophage polarization, and neutrophil chemotaxis. By integrating current evidence and bioinformatic analyses, we establish a theoretical framework linking miR-451's molecular mechanisms to its therapeutic applications. This review not only synthesizes the current understanding of miR-451 in inflammatory diseases but also provides critical insights for developing novel diagnostic tools and therapeutic strategies.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism and Applications of Vagus Nerve Stimulation. 迷走神经刺激的机制及应用。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-14 DOI: 10.3390/cimb47020122

Zhen Chen, Kezhou Liu

引用次数: 0

Luteolin in Inflammatory Bowel Disease and Colorectal Cancer: A Disease Continuum Perspective. 木犀草素在炎症性肠病和结直肠癌中的作用：一个疾病连续体的视角。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-14 DOI: 10.3390/cimb47020126

Fang Liu, Cui Guo, Xue Liu, Zhili Gu, Wenxuan Zou, Xuegui Tang, Jianyuan Tang

{"title":"Luteolin in Inflammatory Bowel Disease and Colorectal Cancer: A Disease Continuum Perspective.","authors":"Fang Liu, Cui Guo, Xue Liu, Zhili Gu, Wenxuan Zou, Xuegui Tang, Jianyuan Tang","doi":"10.3390/cimb47020126","DOIUrl":"10.3390/cimb47020126","url":null,"abstract":"Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory condition that may progress to colorectal cancer (CRC), presenting significant challenges to global health. With shifts in lifestyle, the incidence of both conditions continues to rise, underscoring the urgent need for effective treatments. While traditional therapies can be effective, their high recurrence rates and associated adverse reactions limit their broader application. Luteolin, a flavonoid derived from natural plants, has emerged as a promising focus in both IBD and CRC research due to its multi-target therapeutic potential. This article reviews the molecular mechanisms and signaling pathways through which luteolin regulates immune cell differentiation, mitigates inflammation and oxidative stress, modulates gut microbiota, and restores intestinal mucosal barrier function in IBD. In the context of CRC, luteolin demonstrates significant anti-tumor effects by inhibiting cancer cell proliferation, inducing apoptosis, and suppressing cell migration and invasion. Notably, luteolin has demonstrated significant improvements in IBD symptoms by influencing the differentiation of T cell subsets, decreasing the expression of inflammatory mediators, activating antioxidant pathways, and enhancing the structure of gut microbiota. Furthermore, advancements in formulation technology, such as the use of polymer micelles and responsive nanoparticles, have greatly improved the bioavailability and efficacy of luteolin. However, further investigation is needed to address the bioavailability and potential toxicity of luteolin, particularly in the critical transition from IBD to CRC. This article emphasizes the potential of luteolin in the treatment of IBD and CRC and anticipates its promising prospects for future clinical applications as a natural therapeutic agent.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of a Three-Day vs. Six-Day Exposure to Normobaric Hypoxia on the Cardiopulmonary Function of Rats. 3天与6天常压缺氧对大鼠心肺功能的影响。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-14 DOI: 10.3390/cimb47020125

Charly Bambor, Sarah Daunheimer, Coralie Raffort, Julia Koedel, Aida Salameh, Beate Raßler

{"title":"Effects of a Three-Day vs. Six-Day Exposure to Normobaric Hypoxia on the Cardiopulmonary Function of Rats.","authors":"Charly Bambor, Sarah Daunheimer, Coralie Raffort, Julia Koedel, Aida Salameh, Beate Raßler","doi":"10.3390/cimb47020125","DOIUrl":"10.3390/cimb47020125","url":null,"abstract":"In rats, normobaric hypoxia significantly reduced left ventricular (LV) inotropic function while right ventricular (RV) function was not impaired. In parallel, the animals developed pulmonary edema and inflammation. In the present study, we investigated whether cardiac function and pulmonary injury would aggravate after three and six days of hypoxia exposure or whether cardiopulmonary reactions to prolonged hypoxia would become weaker due to hypoxic acclimatization. Sixty-four female rats were exposed for 72 or 144 h to normoxia. They received a low-rate infusion (0.1 mL/h) with 0.9% NaCl solution. We evaluated indicators of the general condition, blood gas parameters, and hemodynamic function of the rats. In addition, we performed histological and immunohistochemical analyses of the lung. Despite a significant increase in hemoglobin concentration, the LV function deteriorated with prolonged hypoxia. In contrast, the RV systolic pressure and contractility steadily increased by six days of hypoxia. The pulmonary edema and inflammation persisted and rather increased with prolonged hypoxia. Furthermore, elevated protein concentration in the pleural fluid indicated capillary wall stress, which may have aggravated the pulmonary edema. In conclusion, six days of hypoxia and NaCl infusion place significant stress on the cardiopulmonary system of rats, as is also reflected by the 33% of premature deaths in this rat group.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0