Current Issues in Molecular Biology最新文献_第8页

Recent Advances in the Isolation Strategies of Plant-Derived Exosomes and Their Therapeutic Applications. 植物源性外泌体分离策略及其治疗应用研究进展。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-22 DOI: 10.3390/cimb47030144

Jeong-Geon Mun, Dong-Ha Song, Ji-Ye Kee, Yohan Han

{"title":"Recent Advances in the Isolation Strategies of Plant-Derived Exosomes and Their Therapeutic Applications.","authors":"Jeong-Geon Mun, Dong-Ha Song, Ji-Ye Kee, Yohan Han","doi":"10.3390/cimb47030144","DOIUrl":"10.3390/cimb47030144","url":null,"abstract":"Exosome-like nanovesicles (ELNs) derived from natural products are gaining attention as innovative therapeutic agents due to their biocompatibility, low immunogenicity, and capability to transport bioactive molecules such as proteins, lipids, and nucleic acids. These plant-derived ELNs exhibit structural similarities with mammalian exosomes, making them suitable for drug delivery, microbiome-targeted therapies, and regenerative medicine. Recent studies highlight their potential in treating cancer, inflammation, and metabolic disorders. Additionally, ELNs have applications in cosmetics, agriculture, and the food industry. This review combines the latest advancements in research on plant-derived ELNs, focusing on isolation techniques, pharmacological effects, and therapeutic applications. Although plant-derived ELNs offer promising opportunities, several challenges must be addressed, including standardization, large-scale production, and in vivo efficacy. By summarizing cutting-edge studies and suggesting future directions, we aim to inspire further development of plant-derived ELNs as next-generation therapeutic platforms.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ncRNAs-Mediated Pyroptosis in Cerebral Ischemia-Reperfusion Injury: Pathophysiology, Mechanisms, and Therapeutic Perspectives. 脑缺血再灌注损伤中ncrnas介导的焦亡：病理生理学、机制和治疗观点。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-22 DOI: 10.3390/cimb47030141

Ruiyi Xu, Quan Peng, Wen Chen, Xihua Cheng, Guozuo Wang

{"title":"ncRNAs-Mediated Pyroptosis in Cerebral Ischemia-Reperfusion Injury: Pathophysiology, Mechanisms, and Therapeutic Perspectives.","authors":"Ruiyi Xu, Quan Peng, Wen Chen, Xihua Cheng, Guozuo Wang","doi":"10.3390/cimb47030141","DOIUrl":"10.3390/cimb47030141","url":null,"abstract":"Cerebral ischemia-reperfusion injury (CIRI) is a complex pathological process triggered by transient obstruction of blood flow and subsequent reperfusion, ultimately leading to intracellular disturbances such as oxidative stress, inflammatory responses, and programmed cell death. Among the various types of cell death, pyroptosis (an inflammatory kind of regulated cell death) has received increasing attention due to its involvement in key neurovascular unit cells, including endothelial cells, neurons, microglia, and astrocytes. Intriguingly, accumulating evidence demonstrates that non-coding RNAs (ncRNAs), including long non-coding RNAs, microRNAs, and circular RNAs, can modulate multiple stages of pyroptosis in CIRI. This review synthesizes recent findings on the ncRNAs-regulated pyroptosis in CIRI. We highlight the molecular underpinnings of pyroptotic activation following ischemic injury and discuss how ncRNAs shape these mechanisms. By elucidating the interactions between ncRNAs and pyroptosis-related pathways, we intend to present innovative viewpoints for early diagnosis and the development of potential therapeutic strategies to mitigate CIRI.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological Disease-Modifying Antirheumatic Drugs Decrease Uric Acid Levels in the Sera of Patients with Psoriatic Arthritis. 生物疾病改善抗风湿药物降低银屑病关节炎患者血清尿酸水平

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-22 DOI: 10.3390/cimb47030142

Dijana Perković, Marin Petrić, Maja Maleš, Ivana Erceg Maleš, Mislav Radić

{"title":"Biological Disease-Modifying Antirheumatic Drugs Decrease Uric Acid Levels in the Sera of Patients with Psoriatic Arthritis.","authors":"Dijana Perković, Marin Petrić, Maja Maleš, Ivana Erceg Maleš, Mislav Radić","doi":"10.3390/cimb47030142","DOIUrl":"10.3390/cimb47030142","url":null,"abstract":"Objectives: There are many explanations for increased levels of serum uric acid (SUA) in patients with psoriatic arthritis (PsA), but correlation with different treatment options in PsA is not well elucidated. Our aim was to determine the effects of biological disease-modifying antirheumatic drugs (bDMARDs) on SUA levels in patients with PsA.Materials and methods: We analyzed the data of PsA patients treated with different bDMARDs from January 2007 to June 2021. Patients treated with interleukin-17 (IL-17) inhibitors (secukinumab and ixekizumab) and tumor necrosis factor α (TNFα) inhibitors (golimumab, infliximab, adalimumab, certolizumab pegol, and etanercept) were included.Results: A total of 87 patients were included. The SUA levels decreased in 60 (69%) patients after a 3-6-month-long follow-up, and in 25 (28.7%), we noticed an increase. The average decrease in SUA levels was 9.4 ± 49.5 µmol/L (p = 0.039); for TNFα patients, it was 7.3 ± 59.8 µmol/L (p = 0.386), and for IL-17 patients, it was 12.6 ± 28.4 µmol/L (p = 0.013). The levels of SUA decreased in 81.8% of patients treated with infliximab, as well as in 76% of those treated with secukinumab and in 72.7% of those treated with etanercept. The largest average decrease in SUA levels was recorded in the group treated with golimumab (23 µmol/L).Conclusions: A significant decrease in SUA levels was noticed, especially in patients treated with IL-17 inhibitors. Further studies should identify which bDMARD is the most potent in the lowering of SUA levels. bDMARDs were efficient in PsA disease activity.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus. 荧光还原中和试验：一种新的、快速、有效的方法来表征抗登革热病毒抗体的中和活性。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-21 DOI: 10.3390/cimb47030140

Jiazheng Guo, Jiansheng Lu, Peng Du, Kexuan Cheng, Chao Lei, Yujia Jiang, Meiling Peng, Yating Li, Kaiyue Sun, Changyan Xu, Yunzhou Yu, Chen Gao, Qinglin Kang, Yixiao Zhang, Rong Wang, Zhixin Yang

{"title":"Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus.","authors":"Jiazheng Guo, Jiansheng Lu, Peng Du, Kexuan Cheng, Chao Lei, Yujia Jiang, Meiling Peng, Yating Li, Kaiyue Sun, Changyan Xu, Yunzhou Yu, Chen Gao, Qinglin Kang, Yixiao Zhang, Rong Wang, Zhixin Yang","doi":"10.3390/cimb47030140","DOIUrl":"10.3390/cimb47030140","url":null,"abstract":"Dengue virus (DENV) is a major public health threat in the tropical and subtropical regions of the world. Climate change resulting from global warming is further expanding DENV-endemic areas, adversely affecting public life and health. Despite this, no specific drug against DENV has been developed so far. Vaccines and neutralizing antibodies are the chief preventive and therapeutic tools for managing pathogenic infections. The present study describes the development of a novel fluorescence reduction neutralization test (FRNT) for evaluating the neutralizing activity of antibodies against DENV. This FRNT allows rapid antibody screening. In addition, we calculated the FRNT50 to indicate the neutralizing ability of the antibodies. In contrast to the conventional plaque reduction neutralization assay, the FRNT has a shorter experimental cycle, a simpler operation, and greater objectivity, which can greatly accelerate the research and development process of vaccines and antibodies against DENV.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development for Probiotics Based Insulin Delivery System. 基于益生菌的胰岛素输送系统的开发。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-21 DOI: 10.3390/cimb47030137

Byung Chull An, Jusung Lee, Hye Yeon Won, Yongku Ryu, Myung Jun Chung

{"title":"Development for Probiotics Based Insulin Delivery System.","authors":"Byung Chull An, Jusung Lee, Hye Yeon Won, Yongku Ryu, Myung Jun Chung","doi":"10.3390/cimb47030137","DOIUrl":"10.3390/cimb47030137","url":null,"abstract":"Probiotics show beneficial effects on diabetes mellitus (DM). If probiotics can secrete the recombinant insulins that may help suppress DM development, then it would likely have very few adverse side effects. To produce insulin analogs in bacteria, recombinant insulin (insulin-CBT1) should be the single-chain insulin (SCI) similar to proinsulin. However, insulin-CBT1 should allow the protein to activate insulin receptors directly without the need for proteolytic cleavage. In this study, we evaluated the effect of the flexible linker peptide on the physical and structural characteristics of insulin-CBT1 compared with commercial insulin (c-insulin). In the results, the linker peptide had marked effects on polarity and structure by increasing the α-helix content (19.3%→25.6%). Furthermore, insulin-CBT1 induced MIN6 proliferation 1.75-fold more than c-insulin, whereas differentiation and glucose uptake rates by 3T3-L1 were 39% and 15% lower, respectively. The biological anti-diabetes properties of insulin-CBT1 were well evaluated compared with c-insulin. Furthermore, we first suggest a special method for oral administration of insulin-CBT 1 without damage to the digestive tract. We developed an insulin-CBT1 delivery system using Pediococcus pentosaceus (PP), which has been reported as a potential bacteria in DM. First, insulin-CBT1 was harbored in pCBT2-24, which verified the expression and secretion vector system of PP. We finally confirmed that PP-insulin-CBT1 successfully secreted insulin-CBT1 proteins to culture media. These results presented herein open up new avenues to developing therapeutic options for DM.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Molecular to Clinical Implications of Sleep-Related Breathing Disorders on the Treatment and Recovery of Acute Stroke: A Scoping Review. 从分子到临床意义的睡眠相关呼吸障碍对急性中风的治疗和恢复：一个范围综述。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-21 DOI: 10.3390/cimb47030138

Karol Uscamaita, Olga Parra Ordaz, Imán Yazbeck Morell, Marta García Pla, María-José Sánchez-López, Adrià Arboix

{"title":"From Molecular to Clinical Implications of Sleep-Related Breathing Disorders on the Treatment and Recovery of Acute Stroke: A Scoping Review.","authors":"Karol Uscamaita, Olga Parra Ordaz, Imán Yazbeck Morell, Marta García Pla, María-José Sánchez-López, Adrià Arboix","doi":"10.3390/cimb47030138","DOIUrl":"10.3390/cimb47030138","url":null,"abstract":"(1) Background: The aim of this review is to map research into the molecular mechanisms linking sleep-related breathing disorders (SRBDs) and acute stroke and their clinical and therapeutic implications and to identify existing knowledge gaps to suggest new areas of research. (2) Methods: This review was conducted according to the PRISMA extension for scoping reviews (PRISMA-ScR) and a predetermined protocol shared among all authors. (3) Results: The review of the thirteen studies analyzed provides a focused view of the molecular features about interaction between obstructive sleep apnea (OSA) and acute stroke. Our review identifies and highlights the biomarkers most frequently found to be associated with acute stroke, SRBDs, and their clinical repercussions. (4) Conclusions: The association between the presence of sleep apnea, especially in its severe form, and elevated levels of inflammatory markers in patients with acute stroke is highlighted and new research topics in this area are proposed.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Genes to Healing: The Protective Mechanisms of Poria cocos Polysaccharide in Endometrial Health. 从基因到愈合：茯苓多糖对子宫内膜健康的保护机制。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-21 DOI: 10.3390/cimb47030139

Yongxi Huang, Pupu Yan, Jun Zhu, Yinuo Gong, Man Liu, Haishan Cheng, Tilin Yi, Fuxian Zhang, Xiaolin Yang, Yingbing Su, Liwei Guo

{"title":"From Genes to Healing: The Protective Mechanisms of Poria cocos Polysaccharide in Endometrial Health.","authors":"Yongxi Huang, Pupu Yan, Jun Zhu, Yinuo Gong, Man Liu, Haishan Cheng, Tilin Yi, Fuxian Zhang, Xiaolin Yang, Yingbing Su, Liwei Guo","doi":"10.3390/cimb47030139","DOIUrl":"10.3390/cimb47030139","url":null,"abstract":"The aim of this study is to investigate the therapeutic effect of Poria cocos polysaccharide (PCP) on bovine endometritis. Initially, an inflammation model was induced using LPS-treated bovine endometrial epithelial cells (BEND) to identify the differentially expressed genes (DEGs) between the control and LPS groups by transcriptome sequencing, and GO functional annotation and KEGG enrichment analysis were performed. Subsequently, the mechanism of PCP treatment for endometritis was further evaluated using protein immunoblotting and real-time fluorescence quantitative analysis. Finally, the efficacy of PCP in treating endometritis was evaluated using a rat model of endometritis established with a mixed bacterial infection. The results show that transcriptome sequencing identified 4367 DEGs, with enrichment analysis highlighting the primary influences on the cell cycle and apoptosis signaling pathways. Following treatment of BEND with LPS resulted in cell apoptosis and inflammatory response. However, the introduction of PCP intervention significantly inhibited the progression of apoptosis and inflammation. Animal test results indicate that PCP significantly decreases the levels of serum inflammatory in rats suffering from endometritis and enhances antioxidant capacity. Furthermore, it effectively improved uterine swelling and tissue vacuolization caused by bacterial infection. These findings suggest that PCP could alleviate endometritis by modulating the inflammatory response and suppressing cell apoptosis. Poria cocos polysaccharides demonstrate significant potential for applications in immune modulation, anti-inflammatory responses, and antioxidant activities. Their high safety profile makes them suitable candidates as alternative therapeutic agents for the treatment of endometritis in the veterinary field.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How "Omics" Studies Contribute to a Better Understanding of Fuchs' Endothelial Corneal Dystrophy. “组学”研究如何有助于更好地理解Fuchs的内皮性角膜营养不良。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-20 DOI: 10.3390/cimb47030135

Erika Prašnikar, Spela Stunf Pukl

{"title":"How \"Omics\" Studies Contribute to a Better Understanding of Fuchs' Endothelial Corneal Dystrophy.","authors":"Erika Prašnikar, Spela Stunf Pukl","doi":"10.3390/cimb47030135","DOIUrl":"10.3390/cimb47030135","url":null,"abstract":"Fuchs' endothelial corneal dystrophy (FECD) is a progressive eye disease characterized by accelerated loss of endothelial cells and the development of focal excrescence (guttae) on Descemet's membrane, resulting in cornea opacity and vision deterioration. The development of FECD is assumed to be due to the interplay between genetic and environmental factor risks, causing abnormal extracellular-matrix organization, increased oxidative stress, apoptosis and unfolded protein response. However, the molecular knowledge of FECD is limited. The development of genome-wide platforms and bioinformatics approaches has enabled us to identify numerous genetic loci that are associated with FECD. In this review, we gathered genome-wide studies (n = 31) and sorted them according to genomics (n = 9), epigenomics (n = 3), transcriptomics (n = 15), proteomics (n = 3) and metabolomics (n = 1) levels to characterize progress in understanding FECD. We also extracted validated differentially expressed/spliced genes and proteins identified through comparisons of FECD case and control groups. In addition, highlighted loci from each omics layer were combined according to a comparison with similar study groups from original studies for downstream gene-set enrichment analysis, which provided the most significant biological pathways related to extracellular-matrix organization. In the future, multiomics study approaches are needed to increase the sample size and statistical power to identify strong candidate genes for functional studies on animal models and cell lines for better understanding FECD.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodiversity and Evaluation of Genetic Resources of Some Coffee Trees Grown in Al-Baha, Saudi Arabia. 沙特阿拉伯Al-Baha地区一些咖啡树的生物多样性及遗传资源评价。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-20 DOI: 10.3390/cimb47030136

Fatima Omari Alzahrani, Mohammed Obeid Alshaharni, Gamal Awad El-Shaboury, Abdelfattah Badr

{"title":"Biodiversity and Evaluation of Genetic Resources of Some Coffee Trees Grown in Al-Baha, Saudi Arabia.","authors":"Fatima Omari Alzahrani, Mohammed Obeid Alshaharni, Gamal Awad El-Shaboury, Abdelfattah Badr","doi":"10.3390/cimb47030136","DOIUrl":"10.3390/cimb47030136","url":null,"abstract":"The biodiversity of 12 coffee (Coffea arabica L.) cultivars collected from the Al-Baha region in the southwest of Saudi Arabia was evaluated using 25 morphological variations and genetic diversity as demonstrated by molecular polymorphism generated by eight Inter Simple Sequence Repeats (ISSRs) and nine Start Codon Targeted (SCoT) primers. Substantial variations were scored in the morphological traits reflected in the clustering of the examined cultivars in PCA of the coffee cultivars. The examined cultivars were grouped in two groups, one included the cultivars coded Y5, Y6, R113, and Y7 and the other group comprised two clusters; one comprised cultivars coded R8 and R4 and the other comprised cultivars R112, R114, and Y2. In the meantime, the cultivars coded R9 and R111 were differentiated together from other cultivars, while the Y3 cultivar was confirmed by the analysis of ISSR data and SCoT data, which also support the grouping of R9 and R111 cultivars. Principle Component Analysis (PCA) of morphological, ISSR, and SCoT data as a combined set differentiated the examined species into four groups in a scatter plot in agreement with their separation in the cluster trees. The diversity profile among the examined C. arabica cultivars proved that R111 and R4 cultivars are highly diverse, while R8 and Y5 cultivars exhibit low diversity. Alpha diversity indices indicated that R9 and R111 cultivars are the most dominant and stable C. arabica cultivars among the examined samples in the study region.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Dysfunction and Its Potential Molecular Interplay in Hypermobile Ehlers-Danlos Syndrome: A Scoping Review Bridging Cellular Energetics and Genetic Pathways. 超移动ehers - danlos综合征的线粒体功能障碍及其潜在的分子相互作用：连接细胞能量学和遗传途径的范围综述。

IF 2.8 3区生物学

Current Issues in Molecular Biology Pub Date : 2025-02-19 DOI: 10.3390/cimb47020134

Purusha Shirvani, Arash Shirvani, Michael F Holick

{"title":"Mitochondrial Dysfunction and Its Potential Molecular Interplay in Hypermobile Ehlers-Danlos Syndrome: A Scoping Review Bridging Cellular Energetics and Genetic Pathways.","authors":"Purusha Shirvani, Arash Shirvani, Michael F Holick","doi":"10.3390/cimb47020134","DOIUrl":"10.3390/cimb47020134","url":null,"abstract":"Hypermobile Ehlers-Danlos Syndrome (hEDS) is a hereditary connective tissue disorder characterized by joint hypermobility, skin hyperextensibility, and systemic manifestations such as chronic fatigue, gastrointestinal dysfunction, and neurological symptoms. Unlike other EDS subtypes with known genetic mutations, hEDS lacks definitive markers, suggesting a multifactorial etiology involving both mitochondrial dysfunction and non-mitochondrial pathways. This scoping review, conducted in accordance with the PRISMA-ScR guidelines, highlights mitochondrial dysfunction as a potential unifying mechanism in hEDS pathophysiology. Impaired oxidative phosphorylation (OXPHOS), elevated reactive oxygen species (ROS) levels, and calcium dysregulation disrupt cellular energetics and extracellular matrix (ECM) homeostasis, contributing to the hallmark features of hEDS. We reviewed candidate genes associated with ECM remodeling, signaling pathways, and immune regulation. Protein-protein interaction (PPI) network analyses revealed interconnected pathways linking mitochondrial dysfunction with these candidate genes. Comparative insights from Fabry disease and fragile X premutation carriers underscore shared mechanisms such as RNA toxicity, matrix metalloproteinases (MMP) activation, and ECM degradation. These findings may suggest that mitochondrial dysfunction amplifies systemic manifestations through its interplay with non-mitochondrial molecular pathways. By integrating these perspectives, this review provides a potential framework for understanding hEDS pathogenesis while highlighting latent avenues for future research into its molecular basis. Understanding the potential role of mitochondrial dysfunction in hEDS not only sheds light on its complex molecular etiology but also opens new paths for targeted interventions.","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0