Current Issues in Molecular Biology最新文献

{"title":"Research Progress on the Relationship Between PRPF8 and Cancer.","authors":"Guoqing Huang, Dandan Wang, Jiaying Xue","doi":"10.3390/cimb47030150","DOIUrl":"10.3390/cimb47030150","url":null,"abstract":"<p><p>Alternative splicing (AS) plays a crucial role in regulating gene expression and protein diversity, influencing both normal cellular function and pathological conditions, including cancer. Protein pre-mRNA processing factor 8 (PRPF8), a core component of the spliceosome, is integral to the splicing process, ensuring accurate gene transcription and spliceosome assembly. Disruptions in PRPF8 function are linked to a variety of cancers, as mutations in this gene can induce abnormal splicing events that contribute to tumorigenesis, metastasis, and drug resistance. This review provides an in-depth analysis of the mechanisms by which PRPF8 regulates tumorigenesis through AS, exploring its role in diverse cancer types, including breast, liver, myeloid, and colorectal cancers. Furthermore, we examine the molecular pathways associated with PRPF8 dysregulation and their impact on cancer progression. We also discuss the emerging potential of targeting PRPF8 in cancer therapy, highlighting challenges in drug development.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Protective Effects of Perch Essence Against Muscle Atrophy in Cancer Cachexia and Cisplatin Treatment.","authors":"Shu-Lan Yeh, Pei-Yin Chen, Jiunn-Wang Liao, Ruo-Li Huang, Shu-Han Yu, Ling-Ni Chen, Mao-Hsiang Lee, Li-Wen Chen, Haw-Wen Chen, Ya-Chen Yang, Yu-Ling Wu, Kai-Li Liu","doi":"10.3390/cimb47030152","DOIUrl":"10.3390/cimb47030152","url":null,"abstract":"<p><p>Muscle atrophy, through several pathways including increased protein catabolism, leads to adverse effects in cachexia induced by cancer and chemotherapy. Perch essence (PE) is a perch extract rich in branched-chain amino acids and peptides. The present study initially investigated the effects of PE supplementation on muscle atrophy in a mouse model of cancer cachexia induced by C26 cancer cells and compared these effects with those of tryptone. Compared with the tumor-only group, we found that PE supplementation significantly improved body weight, muscle mass, maximum limb grip strength (MLGS), and myosin heavy chain expression in the muscles of tumor-bearing mice. PE also significantly inhibited the expression of factors related to protein degradation, oxidative stress, and inflammation, while enhancing the expression of antioxidant enzymes in tumor-bearing mice. These effects of PE were associated with an increased expression of phosphorylated Akt and forkhead box protein O1, along with a reduced expression of phosphorylated nuclear factor-κB p65 in the muscles of tumor-bearing mice. Furthermore, PE similarly increased MLGS and attenuated muscle atrophy in mice exposed to cisplatin by inhibiting protein degradation. All the therapeutic effects of PE supplementation mentioned above were generally greater than those of tryptone supplementation. These results suggest the potential of PE in protecting against muscle atrophy induced by tumors or chemotherapy.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Association Study for Nodule Traits in Guar.","authors":"Shubham Malani, Waltram Ravelombola, Aurora Manley, Hanh Pham, Madeline Brown, Md Mezanur Rahman","doi":"10.3390/cimb47030151","DOIUrl":"10.3390/cimb47030151","url":null,"abstract":"<p><p>Guar [<i>Cyamopsis tetragonoloba</i> (L.) Taub] is a diploid legume crop cultivated for galactomannan (guar gum) extracted from the endosperm of the seed. Previous studies have suggested that nodulation of guar can be poor in field conditions; however, solid proof has yet to be provided. The objectives of this study were to conduct a genome-wide association study (GWAS) and to identify single nucleotide polymorphism (SNP) markers associated with nodules in guar. GWAS was performed on a total of 225 guar genotypes using 19,007 filtered SNPs. Tassel 5 was used to run five models: single marker regression (SMR), generalized mixed linear model with PCA as a covariate (GLM_PCA), generalized mixed linear model with Q matrix as a covariate (GLM_Q), mixed linear model with PCA and Kinship (K) as covariates (GLM_PCA + K), and mixed linear model with Q and K as covariates (MLM_Q + K). Across all statistical models, the results showed a total of 3, 2, 25, 7, 2, and 3 SNPs were associated with plant height, nodule number per plant, fresh nodule weight, dry nodule weight, fresh aboveground plant biomass, and dry aboveground plant biomass. These SNPs could be used as a tool to select for better nodule traits in guar.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Benefits of Adding Alendronate, Celecoxib, Itraconazole, Ramelteon, and Simvastatin to Endometrial Cancer Treatment: The EC5 Regimen.","authors":"Richard E Kast","doi":"10.3390/cimb47030153","DOIUrl":"10.3390/cimb47030153","url":null,"abstract":"<p><p>Metastatic endometrial cancer continues to be a common cause of death as of 2024, even after maximal use of all currently available standard treatments. To address this problem of metastatic cancer generally in 2025, the drug repurposing movement within oncology identifies medicines in common general medical use that have clinical or preclinical experimental data indicating that they interfere with or inhibit a specific growth driving element identified in a given cancer. The drug repurposing movement within oncology also uses data from large scale in vitro screens of thousands of drugs, looking for simple empirical growth inhibition in a given cancer type. This paper outlines the data showing that five drugs from general medical practice meet these evidence criteria for inhibition of endometrial cancer growth, the EC5 regimen. The EC5 regimen uses the osteoporosis treatment drug, alendronate; the analgesic drug, celecoxib; the antifungal drug, itraconazole; the sleep aid, ramelteon; and the cholesterol lowering drug, simvastatin. Side effects seen with these drugs are usually minimal and easily tolerated by patients.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement and Mechanism of Ergosterol Biosynthesis in Termite Ball Fungus <i>Athelia termitophila</i> by Methyl Jasmonate.","authors":"Yong-Gang Fang, Zahid Khan, Fang-Cheng Hu, Xiao-Hong Su, Lian-Xi Xing","doi":"10.3390/cimb47030149","DOIUrl":"10.3390/cimb47030149","url":null,"abstract":"<p><p>Ergosterol is a component of fungal cell membranes that has physiological functions and applications in drugs, such as anti-inflammatory, anti-tumor, anti-fungal, and other immunosuppressive activities. The fungus <i>Athelia termitophila</i>, also known as the termite ball fungus, primarily contains secondary metabolites (like active ingredients) that are similar to ergosterol. To enhance the synthesis of ergosterol and mycelial biomass in termite ball fungus, methyl jasmonate and salicylic acid were used to stimulate the biosynthesis of ergosterol compounds during the growth of TMB mycelium and relative quantitative levels of gene transcripts. The conditions of the inducers were optimized. Under 80 µmol/L MJ incubation conditions, the content of ergosterol compounds in TMB was increased by 2.23-fold compared with the wild-type strain. RT-qPCR results at the transcriptional level of ergosterol synthesis pathway genes showed that MJ significantly induced the expression of <i>HMGR</i> (3-Hydroxy-3-Methylglutaryl-Coa Reductase), <i>HMGS</i> (3-Hydroxy-3-Methylglutaryl-Coa Synthase), <i>SE</i> (Squalene Epoxidase), and <i>FPS</i> (Farnesyl Diphosphate Synthase) genes in the ergosterol synthesis pathway. For expression levels at different induction days, we collected 7/10 d and 4/6/8 d samples with similar expression patterns, as well as <i>SS</i> (Squalene Synthase)/<i>FPS</i> (Farnesyl Diphosphate Synthase), <i>SE</i> (Squalene Epoxidase)/<i>MVD</i> (Mevalonate Diphosphate Decarboxylase), and <i>HMGS</i> (3-Hydroxy-3-Methylglutaryl-Coa Synthase)/<i>HMGR</i> (3-Hydroxy-3-Methylglutaryl-Coa Reductase) genes with similar expression patterns, which resulted in gene transcription data during ergosterol content synthesis. The current study provides an effective method to increase the ergosterol contents in termite ball fungus and a new idea for the mechanism of MJ-induced ergosterol compound biosynthesis.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dapansutrile Regulates Mitochondrial Oxidative Stress and Reduces Hepatic Lipid Accumulation in Diabetic Mice.","authors":"Ying Wu, Jiaqiang Zhou","doi":"10.3390/cimb47030148","DOIUrl":"10.3390/cimb47030148","url":null,"abstract":"<p><p>(1) Background: Hepatic lipid accumulation is the initial factor in metabolic-associated fatty liver disease (MAFLD) in type 2 diabetics, leading to accelerated liver damage. The NOD-like receptor protein 3 (NLRP3) inflammasome plays a critical role in this process. Dapansutrile (DAPA) is a novel NLRP3 inflammasome inhibitor; however, its effect on ectopic lipid accumulation in the liver remains unclear. This study aimed to investigate the therapeutic effect of DAPA on hepatic lipid accumulation in a diabetic mouse model and its potential mechanisms. (2) Methods: The effects of DAPA on hepatic ectopic lipid deposition and liver function under metabolic stress were evaluated in vivo using db/db and high-fat diet (HFD) + streptozotocin (STZ) mouse models. Additionally, the role and mechanism of DAPA in cellular lipid deposition, mitochondrial oxidative stress, and inflammation were assessed in HepG2 cells treated with free fatty acids (FFA) and DAPA. (3) Results: Our findings indicated that DAPA treatment improved glucose and lipid metabolism in diabetic mice, particularly addressing liver heterotopic lipid deposition and insulin resistance. DAPA treatment also ameliorated lipid accumulation and mitochondrial-related functions and inflammation in HepG2 cells through the NLRP3-Caspase-1 signaling axis. (4) Conclusions: Targeting NLRP3 with DAPA may represent a novel therapeutic approach for diabetes-related fatty liver diseases.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Validation of Key Biomarkers in the Proximal Aqueous Humor Outflow Pathway.","authors":"Rong Du, Enzhi Yang, Madison Clark, Ningli Wang, Yiqin Du","doi":"10.3390/cimb47030147","DOIUrl":"10.3390/cimb47030147","url":null,"abstract":"<p><p>Glaucoma is a leading cause of irreversible blindness, with elevated intraocular pressure (IOP) as the most important risk factor. The trabecular meshwork (TM) and Schlemm's canal are the main components of the proximal aqueous humor outflow pathway. Their dysfunction is a major contributor to IOP elevation. This study aims to identify and validate key biomarkers for TM and Schlemm's canal endothelial (SCE) cells. A Microarray was performed on characterized human TM and SCE cells to analyze their transcriptome profiling. Differentially expressed genes (DEGs) were identified and cross-referenced with published single-cell RNA sequencing (scRNA-Seq) datasets to ensure cell-specific relevance. Further validation was performed using qPCR and re-confirmed on the scRNA-seq datasets. One-way ANOVA was used for statistical analysis, and <i>p</i> < 0.05 was considered significant. The Microarray revealed 341 DEGs, with TM cells enriched in metabolic and signaling pathways and SCE cells enriched in adhesion, immune, and morphogenesis-related processes. Cross-referencing with scRNA-Seq data refined the list of candidate biomarkers, and qPCR confirmed the significant gene expression differences between TM and SCE cells. CTTNBP2 and MGARP were identified as TM cell markers. JAM2, PODXL, and IFI27 are new SCE cell biomarkers. The validated biomarkers offer insights into glaucoma pathophysiology and lay the groundwork for targeted therapies.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Analysis and Fingerprint Construction for <i>Isatis indigotica</i> Fort. Using SSR Markers.","authors":"Xiangyu Xing, Haijun Xu, Yan Dong, Hanwen Cui, Mingrui Sun, Hong Wang, Yang Liu, Li Meng, Chunying Zheng","doi":"10.3390/cimb47030146","DOIUrl":"10.3390/cimb47030146","url":null,"abstract":"<p><p><i>Isatis indigotica</i> Fort. is a traditional medicinal plant, which has anti-inflammatory, antioxidant, and antiviral properties. Despite the development and licensing of several cultivars in recent years, morphological similarity among cultivars complicates their identification. The genetic diversity within <i>I. indigotica</i> significantly impacts the biosynthesis of bioactive substances. To elucidate genetic relationships and evaluate bioactive compounds, <i>I. indigotica</i> cultivars were analyzed using SSR markers. A total of 109 alleles were identified across 29 cultivars at 20 SSR loci, exhibiting a genetic diversity with an average polymorphic information content (PIC) of 0.46. Phylogenetic, principal coordinate analysis (PCoA), and Bayesian clustering revealed that genetic relationships were largely independent of geographic origin, potentially due to regional transplantations. Notably, some cultivars with distinct leaf sizes showed clear genetic differentiation, highlighting their potential as candidates for quality evaluation. A fingerprint was successfully constructed using five SSR markers. These findings provide technical support for cultivar identification, quality evaluation, and intellectual property protection of <i>I. indigotica</i> cultivars.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study of Variation in the Major Phenolic Acid Components of Dandelions Across Different Regions, and the Potential Molecular Mechanisms of Their Anti-Inflammatory Activity.","authors":"Zhaojia Li, Ran Meng, Wei Feng, Zhe Wu, Xuelin Lu, Xiuping Wang, Liangdan Sun","doi":"10.3390/cimb47030145","DOIUrl":"10.3390/cimb47030145","url":null,"abstract":"<p><p>This study explores the variation in the content of major phenolic acid components in dandelions from different regions, and the potential molecular mechanisms underlying their anti-inflammatory activity. High-performance liquid chromatography (HPLC) was employed to analyze dandelion leaves collected from four different regions in Hebei Province across eight harvest periods. The results indicated that chlorogenic acid had the highest content (0.334-1.963%), suggesting that this could be a key evaluation index for dandelion leaf harvesting. Further molecular docking and molecular dynamics simulations revealed that chlorogenic acid, caffeic acid, and chicoric acid could competitively bind to the key amino acid residues (e.g., PHE-151, ILE-117) of the MD-2 protein, thereby preventing the insertion of lipopolysaccharides (LPSs) and inhibiting the formation of the TLR4/MD-2 complex, which elucidates their potential anti-inflammatory mechanism. Moreover, environmental factors significantly influenced the accumulation of phenolic acids in dandelions, with temperature, precipitation, soil pH, and altitude showing correlations with the content variation of major phenolic acids. These findings provide a scientific basis for determining the optimal harvesting period of dandelion leaves, and offer new insights into the anti-inflammatory mechanisms of phenolic acids.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic Activity and Nutrient Profile Assessment of Three <i>Pleurotus</i> Species Under Pasteurized <i>Cenchrus fungigraminus</i> Cultivation.","authors":"Nsanzinshuti Aimable, Hatungimana Mediatrice, Irambona Claude, Jules Biregeya, Yingping Hu, Hengyu Zhou, Penghu Liu, Jing Li, Zhanxi Lin, Guodong Lu, Dongmei Lin","doi":"10.3390/cimb47030143","DOIUrl":"10.3390/cimb47030143","url":null,"abstract":"<p><p>Oyster mushrooms are regarded as one of the most significant edible mushrooms in terms of commercial value because of their rich nutritional profile. Many bioactive extracts from <i>Pleurotus</i> species have been found to exhibit antitumor and antioxidant activities. However, to grow oyster mushrooms in this study, the pasteurized <i>Cenchrus fungigraminus</i> was used as culture material, a type of grass that proliferates and has a high root growth rate. It contains high levels of sugar and protein and yields a large amount of biomass. Because of these characteristics, it is considered an efficient and cost-effective energy crop with various applications, including phytoremediation and fodder production. A pasteurization technique for this grass that is suited for the simplest formulation is simple and cost-effective for growing oyster mushrooms on small farms. This study used pasteurized <i>Cenchrus fungigraminus</i> as a substrate to grow three mushroom species: <i>Pleurotus ostreatus</i>, <i>Pleurotus pulmonarius</i> and <i>Pleurotus florida</i>. The aim was to evaluate their enzyme activities, growth rate, and yields. The findings demonstrated that the average growth rate of three species grown in pasteurized <i>C. fungigraminus</i> was between 25 days and 36 days. Therefore, the mycelium growth rate of <i>P. ostreatus</i> was faster than other <i>pleurotus</i> species in this study. The highest biological efficiency was recorded with <i>P. ostreatus</i> at 78.23%, then <i>P. pulmonarius at</i> 59.88, and lastly, 39.66% <i>P. florida</i>. The changes in five enzyme activities in distinct developmental stages of three different <i>pleurotus</i> species were evaluated. Therefore, the laccase had the highest peak with 13.8 U/g on the 20th day during the growth phase and gradually decreased to the fruiting body stage of <i>P. ostreatus</i>. The expression of manganese peroxidase reached the highest activity of 3.6 U/g in <i>P. ostreatus</i> compared to <i>P. florida</i> and <i>P. pulmonarius</i> on the 10th day. The expression of other enzymes varied between species and developmental phases. The results indicate the usefulness of pasteurized <i>C. fungigraminus</i> for cultivating <i>Pleurotus</i> species and expression enzyme activity in different <i>Pleurotus</i> species.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":"47 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11941170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}