Crop Protection最新文献

{"title":"Cassava crop disease prediction and localization using object detection","authors":"Josephat Kalezhi,&nbsp;Langtone Shumba","doi":"10.1016/j.cropro.2024.107001","DOIUrl":"10.1016/j.cropro.2024.107001","url":null,"abstract":"<div><div>In agriculture, early detection and localization of plant diseases in time using deep learning techniques can help farmers contain the spread of plant diseases. In this work, we apply object detection models to identify and localize various categories of cassava plant leaf diseases. These include You Only Look Once (YOLO) as well as Generalized Efficient Layer Aggregation Network(GELAN) models. We applied YOLO v9-e, YOLO v9-c, as well as GELAN-e and GELAN-c models. The models were successfully trained using a custom cassava dataset. Several evaluation indicators that include precision, recall and mean average precision(mAP) were analysed result. The results have been compared with an earlier version of YOLO model and show an improvement in evaluation indicators reaching above 80% in the majority of diseases.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"187 ","pages":"Article 107001"},"PeriodicalIF":2.5,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-efficiency fungicide screening and field control efficacy of maize southern corn rust","authors":"Yigeng Wang ,&nbsp;Yifei Shao ,&nbsp;Xiaoyan Zhao ,&nbsp;Qinghui Pei ,&nbsp;Ning Nan ,&nbsp;Fengwen Zhang ,&nbsp;Xingyin Jiang ,&nbsp;Xiangdong Li","doi":"10.1016/j.cropro.2024.106997","DOIUrl":"10.1016/j.cropro.2024.106997","url":null,"abstract":"<div><div>Southern corn rust caused by <em>Puccinia dothidea</em> is becoming one of the main diseases of maize planting in China, which seriously threatens the yield of maize in China. Currently, there is no registered pesticide to control southern corn rust in China. Therefore, it is urgent to screen out efficient pesticides to control southern corn rust and reduce the harm of multistem rust to maize yield in China. In the present study, the indoor virulence determination of spore-germination method and 2 years of field efficacy tests were used to screen the fungicides with good prevention and control efficacy on maize southern rust. The results of the indoor toxicity test in 2023 showed that the EC₅₀ of <em>P. dothidea</em> to benzoflumizole was 0.0085 mg/L, and the sensitivity to thifluzamide was relatively poor, with an EC₅₀ of 1.2585 mg/L. The mixture of pyraclostrobin and benzoflumizole at a ratio of 4:1 had the optimal synergistic effect, with an EC₅₀ of 0.0181 mg/L and a cotoxicity coefficient of 128.36. The results of the field efficacy test revealed that 60 g/ha benzovinfluconazole had a good field control effect, and the control effect was 83.09%–85.95%. The combination of 30 g/ha benzodifluconazole and 93.75 g/ha pyraclostrobin had the optimal control effect, and the control effect was 86.92%–88.58% and safe for corn. The results indicated that the application of benzovindiflupyr and its mixture with pyraclostrobin had a good effect on the prevention and control of southern corn rust, which could provide a theoretical basis for the prevention and control of southern corn rust in production and scientific medication.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"187 ","pages":"Article 106997"},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic characterization of advanced cotton lines for resistance to defoliating pathotype of Verticillium dahliae","authors":"Mehmet Aydoğdu ,&nbsp;Metin Durmuş Çetin ,&nbsp;Selfinaz Kübra Velioğlu ,&nbsp;İlker Kurbetli","doi":"10.1016/j.cropro.2024.107000","DOIUrl":"10.1016/j.cropro.2024.107000","url":null,"abstract":"<div><div>Verticillium wilt caused by <em>Verticillium dahliae</em> is one of the most devastating diseases of cotton worldwide but little is known about resistance of cotton to <em>V</em>. <em>dahliae</em> for phenotypic quantifying. The aim of the current study was to phenotypically characterize resistance of 18 advanced cotton lines to defoliating pathotype (Vd-34) of <em>V</em>. <em>dahliae</em>. Experiments were set up in a greenhouse in two consecutive years. Phenotyping of plant response to <em>V</em>. <em>dahliae</em> was quantified by assessing five agronomic traits (defoliation rate, plant height, root weight, stem diameter, biomass) and internal vascular discoloration. Reaction types were established using area under the disease progress curve (AUDPC). The defoliating pathotype Vd-34 induced significant (P ˂ 0.01) reductions in all the examined agrononic traits. Overall reductions in root weight, defoliation rate, plant height, biomass and stem diameter were detected as 42.33, 34.13, 33.56, 32.31 and 26.63%, respectively. Based on the AUDPC values, of the 18 advanced cotton lines, AntV-17, AntV-19 and AntV-20 were detected as moderately resistant to the defoliating pathotype of <em>V</em>. <em>dahliae</em>, while the others showed moderately susceptible, susceptible and very susceptible reactions. The disease quantifying parameters (AUDPC, rAUDPC and vascular disease score) significantly (P ˂ 0.01) and positively correlated with the reductions in the examined agronomic traits. To our knowledge, this is the first detailed phenotyping for resistance to defoliating pathotype of <em>V</em>. <em>dahliae</em> in cotton and reveals new knowledge to quantify cotton resistance to <em>V</em>. <em>dahliae</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"187 ","pages":"Article 107000"},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveys of virus diseases and molecular identification of viruses affecting pepper crops (Capsicum spp.) in southern Benin","authors":"Antoine Abel Missihoun ,&nbsp;André Antoine Fanou ,&nbsp;Chimène Nadège Mahoussi Nanoukon ,&nbsp;Ignace Relique Agbo ,&nbsp;Paulin Sedah ,&nbsp;Mongane Fays ,&nbsp;Nicolas Desoignies","doi":"10.1016/j.cropro.2024.106999","DOIUrl":"10.1016/j.cropro.2024.106999","url":null,"abstract":"<div><div>Boosting the production of market garden products is a top priority for agricultural development in Benin. Chili pepper (<em>Capsicum</em> spp.), an economically important spice, is widely cultivated for its fruits in different parts of the country. Viral diseases are known to have a devastating impact on production. The objective of this study was to identify the viruses associated with pepper cultivation in southern Benin. Surveys and sample collections were conducted in five districts (Ouidah, Kpomassè, Sèmè, Cotonou, and Abomey-Calavi) from three departments (Atlantic, Littoral, and Ouémè) in September 2021. Disease incidence and severity of infection were assessed. Leaf samples from symptomatic and asymptomatic plants were collected randomly from farmers’ fields and were analyzed for viruses by RT-PCR using twelve primer pairs for different viruses sought. The overall high incidence of diseases varied according to the localities between 84.76% and 100% with an average of 94.46%. As for the severity of the disease, it varied significantly depending on the location of 3.24 (in Sèmè-Kpodji) to 2.56 (in Ouidah Cotonou) on average. The analysis of the data obtained by the detection of the presence of viruses in the various samples by RT-PCR shows the following occurrences: <em>potato virus</em> X (PVX) 77.36%, <em>potato virus</em> Y veinal necrosis strain (PVYn) 52.83%, <em>cucumber mosaic virus</em> (CMV) 47.17%, <em>pepper vein virus</em> (PeVYV) 32.08%, <em>potato yellow mosaic virus</em> (PYMV) 32.08%, Polerovirus 32.08%, Begomovirus 32.08%, <em>tomato spoon yellow leaf</em> (TYLCV) 18.87%, <em>tomato spotted wilt virus</em> (TSWV) 15.09%, <em>pepper mottle virus</em> (PepMoV) 11.32%, <em>pepper mild mottle virus</em> (PMMoV) 03.77%, and PVY 0.00% in the five districts surveyed during the periods under survey. Of the twelve groups of viruses searched for in the samples, eleven were found. Each of the samples is infected with one to seven of the twelve viruses sought. Mixed infections were common in most samples, and the high incidence suggests that the cultivars are highly susceptible to viral infections.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"188 ","pages":"Article 106999"},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of cucurbit yellow stunting disorder virus causing yellowing disease on major gourds in India","authors":"B.S. Bharath ,&nbsp;K. Nagendran ,&nbsp;S. Harish ,&nbsp;G. Karthikeyan","doi":"10.1016/j.cropro.2024.106998","DOIUrl":"10.1016/j.cropro.2024.106998","url":null,"abstract":"<div><div>Cucurbitaceous crops are a key group of vegetables widely cultivated in India. Characterized yellowing symptoms along with interveinal chlorosis, mottling and downward curling were observed in ridge gourd, snake gourd, and bottle gourd along with the presence of whiteflies (<em>Bemisia tabaci</em>). To investigate the infecting virus, RT-PCR assays followed by sequencing and Transmission Electron Microscopy (TEM) analysis were performed. Sanger sequencing, nucleotide sequence analysis, and TEM imaging confirmed the association of cucurbit yellow stunting disorder virus (CYSDV), genus <em>Crinivirus</em> within the family <em>Closteroviridae</em>. This study reports the natural occurrence of CYSDV in ridge gourd, snake gourd, and bottle gourd for the first time in India.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"187 ","pages":"Article 106998"},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ITIMCA: Image-text information and cross-attention for multi-modal cassava leaf disease classification based on a novel multi-modal dataset in natural environments","authors":"Huinian Li ,&nbsp;Baoyu Chen ,&nbsp;Jingjia Chen ,&nbsp;Shuting Li ,&nbsp;Feiyong He ,&nbsp;Yingbiao Hu","doi":"10.1016/j.cropro.2024.106981","DOIUrl":"10.1016/j.cropro.2024.106981","url":null,"abstract":"<div><div>With artificial intelligence and deep learning development, crop disease recognition methods leverage deep networks for automatic feature learning but rely on the volume of training data. Addressing the scarcity of data in agriculture, few-shot learning (FSL) and multi-modal learning have become focal points. However, existing methods are confined to a single modality or insufficiently exploit cross-modal features. To address this, we propose a multi-modal contrastive learning approach integrating images and text to tackle the problem of small-sample recognition. This method combines CLIP multi-modal pre-training with cross-attention, termed ITIMCA. Experimental validation demonstrates the effectiveness of our approach in cassava leaf disease recognition tasks under natural conditions. Experimental results show that the proposed model achieved an accuracy of 78.00%, a precision of 88.48%, a recall rate of 80.00%, and an F1-Score of 79.00% on the cassava leaf disease identification and classification dataset. These results suggest that the proposed network effectively identifies cassava leaf diseases.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"189 ","pages":"Article 106981"},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological and population parameters of Telenomus remus and Trichogramma atopovirilia as biological control agents for Spodoptera frugiperda","authors":"Jaime Gonzalez-Cabrera ,&nbsp;Rosa Elia García-García ,&nbsp;Jorge Luis Vega-Chavez ,&nbsp;Yadira Contreras-Bermudez ,&nbsp;Nallely Mejía-García ,&nbsp;Erika Ángeles-Chavez ,&nbsp;Jorge Antonio Sanchez-Gonzalez","doi":"10.1016/j.cropro.2024.106995","DOIUrl":"10.1016/j.cropro.2024.106995","url":null,"abstract":"<div><div>Maize production faces a significant threat from <em>Spodoptera frugiperda</em> (J. E. Smith) worldwide; historically, <em>Trichogramma pretiosum</em> Riley has served as the primary augmentative egg parasitoid for its biological control. However, since 2004, <em>Telenomus remus</em> Nixon has emerged as a viable global alternative. On a regional scale, in Mexico, <em>Trichogramma atopovirilia</em> (Oatman &amp; Platner) was theorized to outperform <em>T. pretiosum</em>, proving superior in both laboratory and field conditions in 2020. To further the field efficacy of the augmentative use of parasitoids as biological agents of <em>S. frugiperda</em>, we conducted comparisons of biological and population parameters under laboratory-controlled conditions to determine whether <em>T. remus</em> would be more effective than <em>T. atopovirilia</em>, the best <em>Trichogramma</em> parasitoid in Mexico for <em>S. frugiperda</em>. We found that the former parasitoid had statistically significant longer female and male longevity, as well as a higher proportion of female offspring, and greater numerical values in net reproduction rate, intrinsic rate of increase, and finite rate of increase, but no differences were found in the number of days required for both parasitoids to develop from egg to female or egg to male, as well as in the mean generation time and doubling time. Under the conditions of these trials, <em>T. remus</em> had a 47.02% greater intrinsic rate of increase (r_m) and may thus be a more effective biological control agent for <em>S. frugiperda</em> than <em>T. atopovirilia</em>. However, these biological and population parameter comparisons are the first execution worldwide; further studies are needed to validate these promising <em>T. remus</em> results.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"188 ","pages":"Article 106995"},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can repellent crops reduce the abundance of the common vole (Microtus arvalis) as a way to reduce crop damage?","authors":"Josef Suchomel ,&nbsp;Jan Šipoš ,&nbsp;Marta Heroldová","doi":"10.1016/j.cropro.2024.106996","DOIUrl":"10.1016/j.cropro.2024.106996","url":null,"abstract":"<div><div>The common vole is one of the most damaging rodents in agriculture. A number of methods are used to suppress its numbers and limit damage. One option is growing crops that are not suitable for voles; this might limit their numbers and distribution. Through long-term monitoring of common voles throughout the Czech Republic, their abundance in eleven crop types (annual, biennial and perennial crops) was evaluated through active burrow counts. Reference crops were selected from perennials and biennials and annual crops were selected as potential repellents. The perennial crops were clover with alfalfa, which serve as the primary habitat for voles. These crops are where voles are found in their highest densities, and from which they spread to the surrounding crops during periods of high abundance. The biennial winter rape was selected because it provides favourable conditions for voles to overwinter and multiply rapidly in the spring. Compared to perennial crops and winter rape, significantly lower numbers of voles were found in onions, poppy seeds and maize. Additionally, fewer voles were also found in mustard compared to perennial crops. Onion and poppy were the only crops to show a significantly lower abundance of voles in comparison to wheat. The annual crops tested are unattractive to voles and due to the cultivation practices used, they do not even have enough weeds as food. As profitable crops they can be cultivated over sufficiently large areas to potentially prevent the spread of voles to surrounding crops, especially during outbreak years.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"187 ","pages":"Article 106996"},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic Metarhizium anisopliae isolates suppress two-spotted spider mite population and black leaf spot disease while enhancing tomato plant growth","authors":"Seyed Ali Safavi ,&nbsp;Seyedeh Reyhaneh Golzan ,&nbsp;Sina Mesri-Ghahramanloo ,&nbsp;J.P. Michaud","doi":"10.1016/j.cropro.2024.106994","DOIUrl":"10.1016/j.cropro.2024.106994","url":null,"abstract":"<div><div>The entomopathogenic fungus <em>Metarhizium anisopliae</em> (Metchn.) Sorokin can grow endophytically in crops such as tomato, often inhibiting the growth of pests and diseases. Different isolates of <em>M. anisopliae</em> vary in their protective properties and require careful assessment prior to their use in any application. We evaluated isolates WEU01 and WEU02 of <em>M. anisopliae</em> for their ability to colonize tomato plants, promote plant growth, suppress spider mites, and inhibit black spot infections caused by <em>Alternaria alternata</em>. Both fungal isolates grew systemically within tomato plants, persisted in plant vegetative tissues for up to eight weeks, reduced spider mite populations, and were antagonistic to <em>A. alternata in vitro</em> and <em>in planta</em>. After four weeks, endophytic <em>M. anisopliae</em> positively affected leaf area, leaf number, stem length, stem diameter, dry weight, and chlorophyl content. These results illustrate the potential benefits of endophytic <em>M. anisopliae</em> for biological control of pests and diseases on tomato.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"187 ","pages":"Article 106994"},"PeriodicalIF":2.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a closed tube loop-mediated isothermal amplification (LAMP) assay to detect Hirschmanniella oryzae (Tylenchida: Pratylenchidae) in exported aquatic plants","authors":"Arunee Suwanngam ,&nbsp;Philipp H. Schiffer ,&nbsp;Anongnuch Sasnarukkit ,&nbsp;Suradej Siripattanapipong ,&nbsp;Kansiree Jindapunnapat ,&nbsp;Toon Ruang-areerate ,&nbsp;Buncha Chinnasri","doi":"10.1016/j.cropro.2024.106989","DOIUrl":"10.1016/j.cropro.2024.106989","url":null,"abstract":"<div><div>Rice root nematode, <em>Hirschmanniella oryzae</em> is an important plant-parasitic nematode inflicting substantial destruction on economically important agricultural crops worldwide and invading an extensive range of plant hosts, including aquatic plants. However, morphological identification of the nematode has encountered limitations. This study developed a loop-mediated isothermal amplification (LAMP) assay targeting the genetic sequence of <em>H</em>. <em>oryzae</em> 28S ribosomal RNA gene as an alternative method. The LAMP assay demonstrated remarkable speed that the outcome could be achieved within 40 min at an isothermal temperature, 65 °C. The assay could interpret the fluorescence (SYBR safe) and color (SYBR green I) in a pre-reaction mixture, ensuring reliability and minimizing contamination risk during post-amplification. Our assay has high specificity and enables accurate differentiation from other nematode species in diverse aquatic plant samples. The sensitivity was higher than PCR and real-time PCR as low as 4.61 fg/μL. The development of fluorescent and colorimetric closed tube LAMP assay highlights it as a valuable tool that is a simplified, sensitive and rapid diagnostic method for detecting aquatic pest nematode, <em>H. oryzae</em>, at point-of-service level. The implementation of this method holds promise for future applications in plant quarantine departments and offers a significant advancement in the diagnosis of infected plants.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"187 ","pages":"Article 106989"},"PeriodicalIF":2.5,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}