Crop Protection最新文献

{"title":"Contamination by systemic insecticide harms the dispersal ability of the lady beetle Hippodamia variegata","authors":"Menghao Zhang ,&nbsp;Hongchun Yv ,&nbsp;Changchun Dai ,&nbsp;Yanhui Lu","doi":"10.1016/j.cropro.2025.107426","DOIUrl":"10.1016/j.cropro.2025.107426","url":null,"abstract":"<div><div>The lady beetle <em>Hippodamia variegata</em> (Coleoptera: Coccinellidae) is a generalist predator in Xinjiang, China, preying on aphids such as <em>Aphis gossypii</em> (Hemiptera: Aphididae) and other arthropods in agricultural ecosystems. Imidacloprid, the most widely used neonicotinoid insecticide globally, is commonly used to manage sucking pests such as aphids; however, it can have detrimental sublethal effects on some natural enemies. The development of IPM programs against pests requires an evaluation of the side effects of insecticides on important natural enemies. We found that exposure to aphids treated with sublethal levels of imidacloprid (LC₂₀ and LC₅₀) reduced longevity, fecundity, and flight performance of <em>H. variegata</em> adults. Specifically, exposure to aphids treated at the LC₅₀ level reduced the flight distance and flight duration of the lady beetle by 81.9 % and 78.5 %, respectively, compared with the control group. Carbohydrate metabolism in lady beetles fed aphids in the LC₂₀ and LC₅₀ groups was significantly impaired, leading to higher trehalose and glycogen content compared to beetles in the control group that were fed untreated aphids. Moreover, the activity of metabolism enzymes was significantly reduced, although there was no significant effect on the levels of metabolism enzymes. In mark-release-recapture experiments conducted in cotton fields, sublethal concentrations of imidacloprid significantly reduced the dispersal distance of these beetles during the first two days after release. These results demonstrate that sublethal concentrations of imidacloprid have a negative impact on the flight dispersal ability of <em>H. variegata</em>, which could impair the regional biological control services provided by this predator.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107426"},"PeriodicalIF":2.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild sources for host plant resistance to Bemisia tabaci in watermelon: insights from behavioral and chemical analyses","authors":"Petrina McKenzie-Reynolds ,&nbsp;Isiaka A. Owolabi ,&nbsp;Anju Biswas ,&nbsp;Quentin D. Read ,&nbsp;Gaelen R. Burke ,&nbsp;Amnon Levi ,&nbsp;Alvin M. Simmons","doi":"10.1016/j.cropro.2025.107411","DOIUrl":"10.1016/j.cropro.2025.107411","url":null,"abstract":"<div><div>Whitefly infestations, primarily due to <em>Bemisia tabaci</em> (Gennadius) (Hemiptera: Aleyrodidae), pose a serious threat to watermelon production, leading to significant yield losses and increased reliance on chemical pesticides. In this study, genotypes of the desert-watermelon <em>Citrullus colocynthis</em> (L.) Schrader and the cultivated watermelon (<em>C</em>. <em>lanatus</em> (Thunberg) Matsumura &amp; Nakai) were evaluated for resistance to <em>B. tabaci</em> using oviposition and vertical Y-tube olfactometer assays. In addition, gas chromatography-mass spectrometry (GC-MS) analysis of plant volatiles was conducted. Among 13 wild accessions tested, two received statistically less oviposition, while all wild genotypes demonstrated statistically reduced attraction of adult whiteflies to plant volatiles as compared with a susceptible cultivar. GC-MS analysis identified phenol derivatives and sesquiterpenes as dominant volatiles in the <em>C. colocynthis</em> resistant accessions. The volatile blend of the wild United States Plant Introduction (PI)-542616 was dominated by phenolic derivatives, comprising nearly the entire profile, while PI-386019 released a greater proportion of antioxidant-rich phenolic esters. These differences highlight clear variation in the relative abundance of major chemical classes among accessions of <em>C. colocynthis</em>, which may contribute to differential resistance against <em>B. tabaci</em>. These findings help elucidate the biochemical and volatile-mediated whitefly-resistance mechanisms in <em>C. colocynthis</em>. By integrating resistant germplasm into integrated pest management (IPM) strategies, results from this research will advance sustainable watermelon production.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107411"},"PeriodicalIF":2.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The whitefly Bemisia tabaci transmits the Pepper yellow leaf curl Indonesia virus to chili plants treated with low-risk insecticides","authors":"Ernawati Djaya ,&nbsp;Iftitah Kartika Amaliah ,&nbsp;Nurul Arfiani ,&nbsp;Melina Melina ,&nbsp;Erwin Najamuddin ,&nbsp;Nurasiah Djaenuddin ,&nbsp;Amran Muis ,&nbsp;Ramlah Arief ,&nbsp;Muhammad Yasin ,&nbsp;Andi Nasruddin","doi":"10.1016/j.cropro.2025.107424","DOIUrl":"10.1016/j.cropro.2025.107424","url":null,"abstract":"<div><div>Pepper yellow leaf curl Indovnesia virus (PepYLCIV) is a damaging disease of chili that can cause total yield loss. The sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), persistently transmits the virus. The insect can also directly damage chili plants by sucking their sap. The study was conducted to determine the effects of inoculation pressure, insecticide dosage, and application timing on whitefly populations and the incidence and severity of PepYLCIV. The results showed that higher inoculation pressures caused increased whitefly survival rates and viral disease transmission. The efficacy of azadirachtin was found to be dose-dependent, with higher concentrations being more effective in reducing both whitefly survival and virus transmission. In contrast, imidacloprid demonstrated higher efficacy across all tested concentrations, eliminating whiteflies and preventing virus transmission. The timing of insecticide application is a crucial factor influencing vector survival and disease incidence. Longer intervals between application and the inoculation access period (IAP) resulted in diminished efficacy of the insecticides. Azadirachtin and imidacloprid provided effective protection against PepYLCIV for up to 2 and 10 days post-application, respectively. Imidacloprid was effective for immediate vector control, while azadirachtin is effective at higher concentrations. Applying imidacloprid at the action threshold level could rapidly reduce the insect population, followed by azadirachtin application to maintain low population levels. The study results could be incorporated into an integrated management strategy to extend imidacloprid's efficacy against <em>B. tabaci</em> and concomitantly reduce its potential impacts on the environment and non-target organisms.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107424"},"PeriodicalIF":2.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of fosthiazate (Nemathorin 10 % GR) in managing Meloidogyne enterolobii in chili crop in Thailand","authors":"Buncha Chinnasri, Natthidech Beesa, Arunee Suwanngam, Kamonwan Puttawong, Thippawan Korsrisakul, Phornthawon Phanbut, Kansiree Jindapunnapat, Anongnuch Sasnarukkit","doi":"10.1016/j.cropro.2025.107425","DOIUrl":"https://doi.org/10.1016/j.cropro.2025.107425","url":null,"abstract":"Root-knot nematodes (<ce:italic>Meloidogyne</ce:italic> spp.) are among the most destructive pathogens affecting chili crops worldwide, causing significant economic losses. Fosthiazate, a nematicide with proven efficacy against plant-parasitic nematodes, has been understudied in Thailand for its impact on root-knot nematodes. This study aimed to evaluate the potential of fosthiazate (Nemathorin 10 % GR) against <ce:italic>Meloidogyne</ce:italic> spp., the causal agent of root-knot disease in chili, under both greenhouse and field conditions. In greenhouse experiments, fosthiazate was applied as preventive and curative treatments at rates of 7.9–31.6 mg/pot (1–4 kg a.i./ha). Both approaches demonstrated significant nematicidal activity against <ce:italic>M. enterolobii</ce:italic>, but preventive treatments consistently yielded higher efficacy. Preventive applications reduced gall formation, egg mass production, and egg counts by 55.3–98.7 %, 75.7–99.6 %, and 64.4–100.0 %, respectively, as compared to without fosthiazate application. Field trials conducted in Khon Kaen and Ubon Ratchathani provinces on “Super Hot” variety (Trial 1) and “Amphawa Hot Pepper” variety (Trial 2), naturally infected with root-knot nematodes further validated these findings. The best results were obtained with two applications of fosthiazate at rates of 2 and 3 kg a.i./ha, leading to reductions in the root-galling index by 22.6 %–59.6 % and egg counts by 49.4 %–80.1 %. Importantly, no phytotoxicity was observed at any application rate or number of application times. These findings suggest that fosthiazate could be a promising option for managing <ce:italic>Meloidogyne</ce:italic> spp. infestations in chili fields. This study provides the first comprehensive evaluation of fosthiazate's effectiveness against root-knot nematodes in chili crops in Thailand and highlights its utility in nematode management strategies.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"27 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition and acaricidal activity of essential oils from Alpinia zerumbet, Bixa orellana, Mesosphaerum suaveolens and Syzygium cumini against Oligonychus punicae on clonal seedlings of Eucalyptus grandis","authors":"Adriele de Castro Ferreira ,&nbsp;Rosenya Michely Cintra Filgueiras ,&nbsp;Paulo Roberto Ramalho Silva ,&nbsp;Izaias Santos Marques ,&nbsp;Antonio José Cantanhede Filho ,&nbsp;Bruno de Araújo Gomes ,&nbsp;Edson Rodrigues Filho ,&nbsp;Franciele Costa de Sousa ,&nbsp;Eslane Reis Farias Mendes ,&nbsp;Helane Cristina De Andrade Rodrigues Severo ,&nbsp;Alyne Freire de Melo ,&nbsp;Kamilla de Andrade Dutra ,&nbsp;Daniela Maria do Amaral Ferraz Navarro ,&nbsp;Solange Maria de França ,&nbsp;Douglas Rafael e Silva Barbosa","doi":"10.1016/j.cropro.2025.107412","DOIUrl":"10.1016/j.cropro.2025.107412","url":null,"abstract":"<div><div><em>Oligonychus (Oligonychus) punicae</em> (Hirst) (Acari: Tetranychidae) is a phytophagous mite that causes significant damage to various crops, including <em>Eucalyptus grandis</em>, where infestations can lead to leaf discoloration, reduced photosynthetic capacity, and overall plant stress. Due to its economic importance, sustainable control strategies are needed. Essential oils (EOs) have emerged as promising tools for managing phytophagous mites, but their effects on <em>O. punicae</em> remain poorly studied. This work evaluated the acaricidal potential of EOs from <em>Alpinia zerumbet</em>, <em>Bixa orellana</em>, <em>Mesosphaerum suaveolens</em>, and <em>Syzygium cumini</em>. EOs were extracted by hydrodistillation and analyzed by GC-MS. Acaricidal activity was assessed through toxicity, ovicidal and repellent tests, and effects on population growth rate. The main constituents (&gt;5 %) were mainly monoterpenoids and sesquiterpenes, with composition varying among species. Toxicity tests showed that at LC₅₀, the oil of <em>B. orellana</em> exhibited the highest lethality (0.55 μL/mL). At LC₉₀, both <em>A. zerumbet</em> and <em>B. orellana</em> were more toxic than <em>S. cumini</em>, with toxicity ratios (TR) of 9.1 and 5.6, respectively. All EOs reduced egg viability by over 50 %, with <em>S. cumini</em> reaching 79.25 % of non-hatched eggs. Population growth was still positive under EO exposure, but all treatments significantly reduced the intrinsic rate of increase compared to the control. All EOs also exhibited repellent activity at sublethal concentrations, with <em>A. zerumbet</em> showing the highest repellency. These results highlight the potential of EOs as effective acaricidal agents against <em>O. punicae</em>. While no single oil excelled in all parameters, each demonstrated specific strengths, supporting their complementary use, individually or in combination, in integrated pest management programs.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107412"},"PeriodicalIF":2.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and insecticidal effects of the essential oil and nanoemulsion of Pogostemon cablin on populations of Sitophilus zeamais","authors":"Álvaro M.O. Santos ,&nbsp;Ana Paula A. Araújo ,&nbsp;Péricles B. Alves ,&nbsp;Arie Fitzgerald Blank ,&nbsp;Eliseu J.G. Pereira ,&nbsp;Raul N.C. Guedes ,&nbsp;Rogéria S. Nunes ,&nbsp;Marcelo C. Picanço ,&nbsp;Virginia E. Masiulionis ,&nbsp;Leandro Bacci","doi":"10.1016/j.cropro.2025.107410","DOIUrl":"10.1016/j.cropro.2025.107410","url":null,"abstract":"<div><div>Insect pests pose significant risks to stored grains worldwide, and while synthetic pesticides are effective, they have environmental and health drawbacks. Plant essential oils (EO), with insecticidal properties, offer a promising alternative. This study evaluated the toxicity of <em>Pogostemon cablin</em> (patchouli) EO and its nanoemulsion on nine populations of <em>Sitophilus zeamais</em>, a major pest of stored grain. Fifteen compounds were identified in the EO, of which patchoulol was the major compound (55.04 %). The EO was tested through topical, contact, and fumigation exposures. Repellency and population growth effects were also assessed. The EO demonstrated fast insecticidal action, with an average median lethal time (LT₅₀) of 53 h. All populations showed similar responses, with the Viçosa population being the most susceptible, and Sete Lagoas the most tolerant. EO and nanoemulsion concentrations required to cause 50 % mortality ranged from 0.30 to 6.37 μL mL⁻¹ (contact) and 0.029–0.037 μL mL⁻¹ (fumigation). The EO and its nanoemulsion were effective repellents against the Viçosa population at LC₅₀ and LC₇₀ levels across all exposure times. For the Sete Lagoas population, repellency was also observed, except for LC₅₀ at 4, 6, and 8 h, and LC₇₀ after 24 h. Increasing nanoemulsion concentrations reduced reproductive index (<em>ri</em>) and grain consumption in both populations. Population decline (<em>ri</em> &lt; 0) began at 50.15 μL kg⁻¹ (Viçosa) and 67.29 μL kg⁻¹ (Sete Lagoas). These results highlight the potential of <em>P. cablin</em> EO for managing <em>S. zeamais</em> in stored grain.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107410"},"PeriodicalIF":2.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of acaricide resistance in Oligonychus punicae (Hirst) (Acari: Tetranychidae) in grapevines from the São Francisco Valley, Brazil","authors":"André S. Melo ,&nbsp;Maria Luiza T. Matheus ,&nbsp;José E.M. Oliveira ,&nbsp;José W.S. Melo ,&nbsp;Herbert A.A. Siqueira ,&nbsp;Manoel G.C. Gondim Jr.","doi":"10.1016/j.cropro.2025.107409","DOIUrl":"10.1016/j.cropro.2025.107409","url":null,"abstract":"<div><div>The intensive use of acaricides, such as abamectin, in the São Francisco Valley region of Brazil has increased selection pressure on the red mite <em>Oligonychus punicae</em>, leading to frequent reports of field control failures. This study evaluated the hypothesis that repeated abamectin use for tetranychid mite control in grapevines has led to a high frequency of resistance in <em>O. punicae</em> populations. This study aimed to detect and document abamectin resistance in <em>O. punicae</em> populations infesting grapevines. To assess potential cross-resistance, toxicity assays were also conducted with bifenthrin and pyridaben—other acaricides registered for <em>Tetranychus urticae</em> control in Brazilian vineyards. Tested populations of <em>O. punicae</em> exposed to a diagnostic concentration of 9 mg L⁻¹ abamectin were classified as resistant, with resistance ratios reaching up to 398-fold relative to the susceptible population. In contrast, the label-recommended concentrations of bifenthrin and pyridaben caused 100 % mortality in all tested populations, although resistance ratios varied from 1- to 25-fold. A significant positive correlation was observed between the LC₉₀ values of abamectin and bifenthrin; however, the results suggest a pattern of multiple resistance rather than cross-resistance among the tested acaricides. Understanding the evolution of acaricide resistance in <em>O. punicae</em> is essential for developing effective pest control and resistance management strategies in viticulture.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107409"},"PeriodicalIF":2.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungicides for the management of blackleg disease of canola caused by Leptosphaeria maculans in the Western Cape province of South Africa","authors":"Huibrecht Maria Schreuder ,&nbsp;Gerhardus Johannes van Coller ,&nbsp;Beatrix Coetzee ,&nbsp;Diane Mostert","doi":"10.1016/j.cropro.2025.107404","DOIUrl":"10.1016/j.cropro.2025.107404","url":null,"abstract":"<div><div>Blackleg disease, caused by <em>Leptosphaeria maculans</em>, severely impacts canola production in the Western Cape. Fungicides are an important component of integrated management, but little information is available on fungicide efficacy under South African conditions. With decreased sensitivity reported for <em>L</em>. <em>maculans</em> to the demethylation inhibitor (DMI) class fungicides in Australia and Europe, it is important that fungicide sensitivity is also evaluated locally. This study screened 118 <em>L. maculans</em> isolates, collected between 2007 and 2022 from canola growing regions of the Western Cape, for <em>in vitro</em> sensitivity to two active ingredients frequently used in canola production, namely tebuconazole and prothioconazole. Field trials were conducted at four locations in 2022 and 2023 to assess the efficacy of six fungicide products on two canola cultivars. Tebuconazole 50% effective concentrations (EC₅₀) ranged from 144.9 ng/mL to 985.3 ng/mL, which was within the range of EC₅₀ values found for sensitive isolates in similar studies. Tebuconazole EC₅₀ values steadily increased from 2020 to 2022, suggesting a shift in sensitivity. Prothioconazole EC₅₀ values were between 270.3 ng/mL and 1620.4 ng/mL. In the field trials, all fungicide products, except the unregistered product Bumper, were effective in lowering blackleg severity. The cultivar Diamond showed a greater fungicide response than the more resistant cultivar 44Y94. Under higher disease pressure, however, fungicide applications also reduced blackleg severity in 44Y94. When sequences of the <em>CYP51</em> promotor region were screened for inserts associated with reduced DMI sensitivity, no inserts were detected in local isolates. This study provides a foundation for the effective and sustainable use of fungicides in an integrated blackleg management strategy in South Africa.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107404"},"PeriodicalIF":2.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"News letter 198","authors":"","doi":"10.1016/j.cropro.2025.107386","DOIUrl":"10.1016/j.cropro.2025.107386","url":null,"abstract":"","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"198 ","pages":"Article 107386"},"PeriodicalIF":2.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and molecular characterization of phytoplasma associated with cherry trees in Himachal Pradesh (India)","authors":"Aman Chauhan ,&nbsp;Anil Handa ,&nbsp;Ajay Brakta ,&nbsp;Pankaj Kumar ,&nbsp;Bhupesh Gupta ,&nbsp;Rakesh Daroch ,&nbsp;Abhinav Thakur","doi":"10.1016/j.cropro.2025.107405","DOIUrl":"10.1016/j.cropro.2025.107405","url":null,"abstract":"<div><div>During the surveys conducted in year 2023–2024 in cherry orchards of Himachal Pradesh, India, several cherry trees were found to be severely infected by a phloem residing pathogen showing the symptoms like little leaf, leaf rolling, leaf reddening, yellowing, tree decline and dieback. Fluorescence based microscopy and PCR assay of the infected plant samples showed the presence of phytoplasma in cherry plants. Presence of bright fluorescent bodies in phloem tissues and sequence analysis of 1239 bp 16S-23S rDNA amplified fragment by nested-PCR confirmed the association of <em>Candidatus</em> phytoplasma ziziphi belonging to 16SrV-B sub-group with declining cherry trees. The present research is the first report of infection by <em>Ca</em>. phytoplasma ziziphi in cherry trees in India.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"198 ","pages":"Article 107405"},"PeriodicalIF":2.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}