Crop Protection最新文献

{"title":"Transgenerational Sublethal Effects of sulfoxaflor on the Suppression of Aphis citricola Van der Goot (Hemiptera: Aphididae)","authors":"Yuntao Feng, Xiaojun Guo, Ya Li, Yameng Zhang, Kang Jia, Runxiang Zhang","doi":"10.1016/j.cropro.2025.107318","DOIUrl":"https://doi.org/10.1016/j.cropro.2025.107318","url":null,"abstract":"The spirea aphid <ce:italic>Aphis citricola</ce:italic> Van der Goot is a serious fruit trees pest in China. Sulfoxaflor is a novel sulfoximine insecticide that has been widely used to control sucking insect pests. This study aimed to investigate the transgenerational sublethal effects of sulfoxaflor on the biological traits and metabolic enzymes activities of <ce:italic>A. citricola</ce:italic>. Our results showed that sublethal concentrations of sulfoxaflor (LC<ce:inf loc=\"post\">10</ce:inf> and LC<ce:inf loc=\"post\">20</ce:inf>) significantly reduced the adult longevity and fecundity of the F<ce:inf loc=\"post\">0</ce:inf> generation. Furthermore, the development time of the first instar nymph and the pre-adult of F<ce:inf loc=\"post\">1</ce:inf> generation were significantly prolonged. The total prereproductive period (TPRP) and the mean generation time (<ce:italic>T</ce:italic>) of the F<ce:inf loc=\"post\">1</ce:inf> generation were also significantly extended. Consequently, the fecundity, intrinsic rate of increase (<ce:italic>r</ce:italic><ce:inf loc=\"post\"><ce:italic>m</ce:italic></ce:inf>), and finite rate of increase (<ce:italic>λ</ce:italic>) of the F<ce:inf loc=\"post\">1</ce:inf> generation were significantly diminished. Additionally, the activities of cytochrome P450 monooxygenases (P450) and carboxylesterase (CarE) in <ce:italic>A. citricola</ce:italic> were enhanced following treatment with sublethal concentrations of sulfoxaflor, indicating their involvement in the detoxification of sulfoxaflor. These results indicate that sublethal concentrations of sulfoxaflor inhibited the development and reproduction of <ce:italic>A. citricola</ce:italic> in both the exposed generation and the subsequent generation, leading to a suppression of population growth over two consecutive generations. This research provides a foundation for a deeper understanding of the intergenerational sublethal effects of sulfoxaflor and suggests that sulfoxaflor has promising potential for controlling <ce:italic>A. citricola</ce:italic>.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"627 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of potato aucuba mosaic virus","authors":"An Yan,&nbsp;Yunting Lei,&nbsp;Zhaoyao Yu,&nbsp;Gaoli Wu,&nbsp;Wenzhuo Feng,&nbsp;Xiangru Chen","doi":"10.1016/j.cropro.2025.107317","DOIUrl":"10.1016/j.cropro.2025.107317","url":null,"abstract":"<div><div>Potatoes, as the fourth largest global food crop, are extensively cultivated worldwide. However, potato production is significantly impacted by plant viruses, among which the potato aucuba mosaic virus (PAMV) is a notable threat. PAMV frequently co-infects with other plant viruses, severely reducing potato yields and posing a risk to the sustainable development of the potato industry. To better control the disease caused by PAMV, a practical detection method is urgently in need. In this study, we developed a one-step reverse-transcription loop-mediated isothermal amplification (one-step RT-LAMP) assay for the detection of PAMV. Primers for the one-step RT-LAMP were designed by targeting conserved regions of all PAMV isolates available in online databases, and the reaction conditions, including temperature, were optimized. Through comparative analysis with conventional RT-PCR and serological tests, we confirmed that the one-step RT-LAMP method developed here is both specific and efficient. Furthermore, the detection limit of the assay was determined to be approximately 2 copies of PAMV, demonstrating its high sensitivity. The practical applicability of the method was validated by testing potato and pepino samples collected from Gansu and Yunnan provinces, with results indicating its effectiveness in field sample detection. Overall, our findings demonstrate that the one-step RT-LAMP method is accurate, reliable, and highly practical, offering a valuable tool for controlling PAMV derived diseases and contributing to the sustainable development of the potato industry.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107317"},"PeriodicalIF":2.5,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric one-step reverse transcription loop-mediated isothermal amplification for rapid and specific detection of sunflower chlorotic mottle virus in sunflower","authors":"Salit Supakitthanakorn,&nbsp;Kanungnit Reanwarakorn","doi":"10.1016/j.cropro.2025.107316","DOIUrl":"10.1016/j.cropro.2025.107316","url":null,"abstract":"<div><div>Sunflower chlorotic mottle virus (SuCMoV), an emerging sunflower-infecting virus in Thailand, was considered as one of the most frequently emerged viruses infecting sunflowers causing chlorotic spots, mottle, yellowing and stunting symptoms. This study aimed to develop and validate the colorimetric one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of SuCMoV in sunflowers. We optimized the RT-LAMP conditions and determined that incubation at 65 °C for 30 min in a heat block incubator provided optimal results, as confirmed by the presence of ladder-like pattern LAMP products visualized through agarose gel electrophoresis. In the colorimetric detection system, positive reactions were clearly indicated by a color change from pink to yellow, while negative reactions remained pink. The specificity test verified that the LAMP primers did not cross-react with other potyviruses. Sensitivity analysis demonstrated that the RT-LAMP assay could detect SuCMoV RNA at concentrations as low as 10 fg/μL which were greater than those of one-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) and one-step RT-PCR. Validation of the colorimetric one-step RT-LAMP assay using naturally infected sunflower samples confirmed its ability to successfully detect SuCMoV, even in mixed viral infections with closely related sunflower-infecting potyviruses detecting by multiplex RT-PCR. To our knowledge, this study represents the first development of a colorimetric one-step RT-LAMP assay for rapid and sensitive detection of SuCMoV.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107316"},"PeriodicalIF":2.5,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular screening and dose-response assays provide insights into pyrethroid resistance in four agriculturally important aphid species","authors":"Daniel J. Leybourne ,&nbsp;Elinor Baird ,&nbsp;Duncan Coston ,&nbsp;Rory Jones ,&nbsp;Nasamu Musa","doi":"10.1016/j.cropro.2025.107315","DOIUrl":"10.1016/j.cropro.2025.107315","url":null,"abstract":"<div><div>Aphids are important pests that cause significant crop damage. In the UK, aphid species of particular concern in cereal systems include the grain aphid (<em>Sitobion avenae</em>), the bird cherry-oat aphid (<em>Rhopalosiphum padi</em>), and the rose-grain aphid (<em>Metapolophium dirhodum</em>); the peach-potato aphid (<em>Myzus persicae</em>) is a species of significant concern across several arable and horticultural cropping systems. Due to the impact aphids have on crop production, managing and controlling populations before they negatively impact yield is key. Pesticides are a central component in many management plans, however tightened regulations have left farmers mainly reliant on a single group of pesticides, the pyrethroids. This has led to an overreliance on pyrethroids, enabling resistance and tolerance to evolve in several species, including <em>S. avenae</em> and <em>M. persicae</em>. Frequent surveys of insect populations can monitor the prevalence of insecticide resistance and identify new species where reduced sensitivity (i.e., tolerance) is developing. These surveys inform farmers and ensure that control methods remain effective. Here, we survey multiple aphid populations for resistance or tolerance to pyrethroids. We use a combination of molecular approaches to identify known resistance mechanisms in <em>S. avenae</em> and <em>M. persicae</em> and dose-response assays to screen for reduced sensitivity in <em>R. padi</em> and <em>M. dirhodum</em>. We identify low prevalence of pyrethroid resistance in <em>S. avenae</em> and high levels of insecticide resistance in <em>M. persicae</em>, with 74 % of <em>M. persicae</em> populations harbouring at least one resistance mechanism. All <em>M. dirhodum</em> populations tested were highly sensitive to pyrethroids, although we identify reduced sensitivity in several <em>R. padi</em> populations, the first report of reduced sensitivity in this species in the UK.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107315"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Botryosphaeria dothidea causes leaf spot disease on Rosa roxburghii in China","authors":"Qinyi Hu ,&nbsp;Taotao An ,&nbsp;Wei Jia ,&nbsp;Junxian Chen ,&nbsp;Ying Xu ,&nbsp;Zhu Li ,&nbsp;Haixia Ding","doi":"10.1016/j.cropro.2025.107312","DOIUrl":"10.1016/j.cropro.2025.107312","url":null,"abstract":"<div><div><em>Rosa roxburghii</em> is a perennial shrub belonging to <em>Rosaceae</em> family, is a resource for both medicinal and edible purposes. In July 2023, a survey was conducted in Guiding (26°459′N, 107°149′E), Guizhou, China. The incidence rate reached 25 % in the field of 100,00 m², which seriously affected the yield and nutritional value of <em>R roxburghii</em>. The main characteristic of the infected leaves is irregular black spots. <em>Botryosphaeria dothidea</em> was isolated from the symptomatic leaves of <em>R roxburghii</em>, and it was identified by morphological and phylogenetic (ITS, TEF1-α, TUB2). Inoculation with the representative isolate B-Y-1 on the leaves can induce leaf spot disease of <em>R roxburghii</em>. Finally, Koch's postulates were established by re-isolation and re-identification of <em>B. dothidea</em> isolates from the inoculated leaves. To our knowledge, this is the first report of leaf spot disease caused by <em>B. dothidea</em> on <em>R roxburghii</em> leaves. These findings will be helpful for formulating the prevention and management strategies for leaf spot disease of <em>R roxburghii</em> in Guizhou.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107312"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of anthracnose caused by Colletotrichum jiangxiense in Epimedium sagittatum (Sieb. et Zucc.) Maxim. from Guizhou Province, China","authors":"Kaiyun Jiang ,&nbsp;Yuanchang Luo ,&nbsp;Xiangsheng Chen ,&nbsp;Shuang Liang ,&nbsp;Wensong Zhang ,&nbsp;Zhong Li","doi":"10.1016/j.cropro.2025.107314","DOIUrl":"10.1016/j.cropro.2025.107314","url":null,"abstract":"<div><div><em>Epimedium sagittatum</em> (Sieb. et Zucc.) Maxim., a perennial medicinal plant belonging to the Berberidaceae family, exhibits therapeutic efficacy by regulating renal physiological functions. From May to October 2024, a severe outbreak of anthracnose was observed in an <em>E. sagittatum</em> plantation in Jianhe County, Guizhou Province, China, with an incidence rate of approximately 25–80 %, causing severe losses, and research regarding anthracnose in <em>E. sagittatum</em> is limited. In this study, we aimed to identify the causative. Integrated molecular analysis of ITS, <em>gapdh</em>, <em>act</em>, <em>tub2</em>, <em>chs-1</em>, and <em>his3</em> and morphological characterisation of conidia identified the isolated strains as <em>Colletotrichum jiangxiense</em>. Furthermore, experimental validation through pathogenicity assessments revealed that the disease symptoms were consistent with those documented under natural field conditions, confirming that <em>C. jiangxiense</em> as the causative pathogen. To our knowledge, this is the first report of <em>C. jiangxiense</em> causing anthracnose in <em>E. sagittatum</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107314"},"PeriodicalIF":2.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological characteristics of high-searching individuals of the Heterorhabditis bacteriophora HBH hybrid strain for use in biocontrol","authors":"Alperen Kaan Bütüner,&nbsp;İsmail Alper Susurluk","doi":"10.1016/j.cropro.2025.107313","DOIUrl":"10.1016/j.cropro.2025.107313","url":null,"abstract":"<div><div>Entomopathogenic nematodes (EPNs) are widely used in biological control to manage insect pests. The present study aimed to select individuals with enhanced host-seeking abilities from the <em>Heterorhabditis bacteriophora</em> HBH hybrid strain using an apparatus and the selected individuals were then compared with the original strain in terms of infectivity, reproductive capacity, and storage survival. Initially, last-stage <em>Galleria mellonella</em> larvae were placed in a plastic cage at the bottom of the apparatus. Subsequently, 1000 infective juveniles (IJs) were transferred to the upper part of the apparatus, and kept at 25 °C. This process was repeated until the fifth generation was obtained. The fifth-generation individuals (termed HBH Plus) were then compared with those of the HBH hybrid strain. The results indicated that the highest efficacy of the selected individuals (HBH Plus) against <em>G. mellonella</em> and <em>Tenebrio molitor</em> larvae, <em>Rhizopertha dominica</em>, and <em>Oryzaephilus surinamensis</em> adults was 90, 73.33, 86.67, and 83.33 %, respectively. For the parental culture, these values were 76.67, 63.33, 73.33 and 73.33 %, respectively. When reproductive capacity was examined, HBH Plus producing an average of 125.333,33 IJs per <em>G. mellonella</em> larva, versus 108.741,67 in the parental strain per <em>G. mellonella</em> larva. An increase in mortality rates in the IJ stages was observed with the extension of the storage duration in both the HBH hybrid strain and HBH Plus individuals. HBH Plus reached a mortality rate of 20.21 % by month five. These findings suggest that selection for enhanced host-seeking behavior in EPNs can improve their biological control potential.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107313"},"PeriodicalIF":2.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life table parameters, consumption, and frass production of Plodia interpunctella on four major cultivars of Turkish hazelnut","authors":"Yunus Emre Altunç ,&nbsp;Hsin Chi ,&nbsp;Ali Güncan","doi":"10.1016/j.cropro.2025.107309","DOIUrl":"10.1016/j.cropro.2025.107309","url":null,"abstract":"<div><div>The Indian meal moth, <em>Plodia interpunctella</em> (Hübner) (Lepidoptera: Pyralidae), is a globally significant pest of stored commodities, including hazelnuts. In this study we investigated life table parameters, consumption, and frass production of <em>P. interpunctella</em> on four major Turkish hazelnut cultivars: ‘Çakıldak’, ‘Mincane’, ‘Palaz’, and ‘Tombul’. The development, fecundity, consumption, and frass production were analyzed using the age-stage, two-sex life table theory. Significant differences in developmental durations were observed among the cultivars, with insects reared on Mincane exhibiting the longest preadult development period (43.51 days). No significant differences were observed in the net reproductive rate (<em>R</em>₀), intrinsic rate of increase (<em>r</em>), and finite rate of increase (<em>λ</em>) among cultivars. The heaviest pupae were recorded on Mincane and Tombul cultivars. Consumption and frass production rates also varied among cultivars. Larvae reared on Palaz exhibited the highest net consumption rate and the lowest net frass production rate. Population projections showed the largest population size on Tombul (&gt;8.1 million individuals, including 4.6 million larvae) after 120 days, highlighting its potential risk in storage conditions. Notably, the highest larval population was on Mincane (&gt;5.6 million individuals). The projected larval population size, frass, and total consumption exhibited similar lower trends on both Çakıldak and Palaz cultivars. These results highlight the significance of hazelnut cultivar selection in the context of <em>P. interpunctella</em> management. Specifically, Çakıldak would be the most suitable choice for integrated pest management due to its lowest preadult survival rate, along with the low predicted pest population size, frass production, and total consumption.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107309"},"PeriodicalIF":2.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of potassium silicate application on barley, Hordeum vulgare for management of corn-leaf aphid, Rhopalosiphum maidis (Fitch) L","authors":"Sunny Maanju ,&nbsp;Surender Singh Yadav ,&nbsp;Poonam Jasrotia ,&nbsp;Manoj Kumar Jat ,&nbsp;Preeti Sharma ,&nbsp;Gyanendra Singh","doi":"10.1016/j.cropro.2025.107311","DOIUrl":"10.1016/j.cropro.2025.107311","url":null,"abstract":"<div><div>Corn-leaf aphid (CLA), <em>Rhopalosiphum maidis</em> (Fitch) prefers barley (<em>Hordeum vulgare</em>) the most and suck its phloem sap. Owing to lack of resistant barley cultivars against <em>R. maidis</em>, the current study was formulated to evaluate the effect of seven silicon (Si) treatments (including control) on feeding preference of <em>R. maidis</em> on detached-barley leaf sections under free and pair choice tests in controlled conditions (20 °C; 70 ± 10 % RH) at ICAR-IIWBR (India). The treatments included different concentrations of potassium and sodium silicate as: T₁-Na₂SiO₃ 0.25 %, T₂-Na₂SiO₃ 0.5 %, T₃-Na₂SiO₃ 1 %, T₄-K₂SiO₃ 0.25 %, T₅-K₂SiO₃ 0.5 %, T₆-K₂SiO₃ 1 % and C-Control. The statistically highest Si deposition was observed in T₆-K₂SiO₃ 1 % with 0.1396 mean absorbance at 650 nm wavelength. In a similar trend, the significantly minimum number of aphids were reported on T₆-K₂SiO₃ 1 % (0.73 and 0.25) which was at-par with T₃-Na₂SiO₃ 1 % (0.88 and 0.40) under free (LSD = 0.34) and pair choice tests (LSD = 0.18), respectively. The nymph production was also significantly lowest in T₆-K₂SiO₃ 1 % with 7.04 nymphs (LSD = 2.42) and highest reduction of 82.05 % in mean number of aphids over control. Overall, potassium silicate was more effective than sodium silicate with T₆-K₂SiO₃ 1 % as the best treatment which can be utilized as an environmentally friendly approach for <em>R. maidis</em> management in barley. This study can form the basis of further investigations on prospects of silicon application for managing CLA under field conditions.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107311"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lasiodiplodia theobromae, Pestalotiopsis clavispora and Fusarium spp. associated with pomegranate dieback and fruit rot in Tunisia","authors":"Hayfa Jabnoun-Khiareddine ,&nbsp;Rania Aydi Ben Abdallah ,&nbsp;Messaoud Mars ,&nbsp;Mejda Daami-Remadi","doi":"10.1016/j.cropro.2025.107310","DOIUrl":"10.1016/j.cropro.2025.107310","url":null,"abstract":"<div><div>Tunisia is one of the main pomegranate (<em>Punica granatum</em> L.) producing countries. Between 2018 and 2021, a 15–20 % incidence of dieback and fruit rot was observed in many pomegranate orchards along Tunisian coasts. Faced with this issue, the current study aimed to characterize the etiological agents associated with these symptoms. Morphological characterization combined with multigene-combined phylogenetic analyses revealed that five fungal species namely <em>Lasiodiplodia theobromae</em>, <em>Neopestalotiopsis clavispora</em>, <em>Fusarium equiseti</em>, <em>F. brachygibbosum</em>, and <em>F. incarnatum</em> were involved in branch dieback, while <em>L</em>. <em>theobromae</em> and <em>F. proliferatum</em> species were associated with pomegranate fruit rot symptoms. As far as our knowledge extends, this is the first report of the involvement of these fungal species in pomegranate dieback and fruit rot in Tunisia and in Africa. Pathogenicity tests carried out through inoculation of detached pomegranate cv. Gabsi shoots and fruits showed that all tested fungal species were pathogenic by inducing disease symptoms with different degrees of severity. Given the rapid and severe progress of these diseases, it is critical to implement prevention and management strategies to alleviate their incidence and reduce tree losses.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107310"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144254375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}