Prevalence of mealybug wilt-associated viruses in pineapple 'Queen Victoria' cultivars in Reunion Island and the potential of virus-free vitroplants for disease management

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-10-10 DOI:10.1016/j.cropro.2025.107440

Delphine Massé , Frédéric Chiroleu , Nathalie Cassam , Bruno Hostachy , Jean-Michel Lett , Pierre Lefeuvre , Pierre-Yves Teycheney

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引用次数: 0

Abstract

Mealybug wilt disease of pineapple (MWP) poses a significant threat to global pineapple production, yet its etiology remains poorly understood. The disease is believed to involve mealybugs, multiple pineapple mealybug wilt-associated viruses (PMWaVs, genus Ampelovirus), and possibly other viruses from the Badnavirus, Secovirus, and Vitivirus genera. This study examines the distribution and prevalence of three ampeloviruses (PMWaV1, PMWaV2, PMWaV3) and two badnaviruses (PBERV, PBCOV), previously associated to MWP symptoms, across 15 pineapple plots of the ‘Queen Victoria’ cultivar in Reunion Island. A total of 450 symptomatic and asymptomatic leaf samples were analyzed using PCR and RT-PCR-based diagnostics. Globally, 93 % of samples were infected with at least one virus, and 76 % were co-infected with two to four viruses. PMWaV1 (78 %) and PBCOV (87 %) were the most prevalent, while PBERV was not detected. Virus presence was associated with leaf dieback, wilting, and curling. Leaf reddening and yellowing, commonly associated with MWP, were not associated with viral infection, suggesting that other biotic or abiotic factors may be responsible for these symptoms. Viral infections and MWP symptoms were significantly lower in plots planted with vitroplants (VPs) compared to those using suckers, highlighting the potential of VPs in reducing viral infections. These findings highlight the complexity of MWP and underscore the potential benefits of using virus-free VPs to manage the disease.

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留尼汪岛“维多利亚女王”菠萝品种粉蚧萎蔫相关病毒的流行及无病毒体外植物用于病害管理的潜力

菠萝粉蚧枯萎病（MWP）对全球菠萝生产构成重大威胁，但其病因尚不清楚。该疾病被认为涉及粉蚧、多种菠萝粉蚧萎蔫相关病毒（pmwas， Ampelovirus属），以及可能来自badnavvirus、Secovirus和Vitivirus属的其他病毒。本研究在留尼旺岛的“维多利亚女王”菠萝品种的15个地块中调查了三种葡萄病毒（PMWaV1、PMWaV2、PMWaV3）和两种坏病毒（PBERV、PBCOV）的分布和流行情况，这两种病毒以前与MWP症状相关。采用基于PCR和rt -PCR的诊断方法，对450份有症状和无症状的叶片样本进行了分析。在全球范围内，93%的样本至少感染了一种病毒，76%的样本同时感染了两到四种病毒。PMWaV1（78%）和PBCOV（87%）最为常见，而PBERV未检出。病毒的存在与叶片枯死、枯萎和卷曲有关。通常与MWP相关的叶片变红和变黄与病毒感染无关，这表明其他生物或非生物因素可能导致这些症状。与使用吸盘的试验田相比，种植体外植株（VPs）的试验田的病毒感染和MWP症状显著降低，突出了体外植株在减少病毒感染方面的潜力。这些发现突出了MWP的复杂性，并强调了使用无病毒vp来控制疾病的潜在益处。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.