Crop Protection最新文献_第2页

Disruptions of mobility and foraging behavior of Alphitobius diaperinus adults exposed to sublethal insecticidal concentrations 暴露于亚致死浓度杀虫剂对成年双翅蚜蝇迁移和觅食行为的影响

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-07-02 DOI: 10.1016/j.cropro.2025.107327

Demeter Lorentha S. Gidari, Nickolas G. Kavallieratos, Maria C. Boukouvala

{"title":"Disruptions of mobility and foraging behavior of Alphitobius diaperinus adults exposed to sublethal insecticidal concentrations","authors":"Demeter Lorentha S. Gidari, Nickolas G. Kavallieratos, Maria C. Boukouvala","doi":"10.1016/j.cropro.2025.107327","DOIUrl":"10.1016/j.cropro.2025.107327","url":null,"abstract":"<div><div>Various pyrethroids and neonicotinoids have been evaluated against <em>Alphitobius diaperinus</em>. However, deltamethrin and a mixture of acetamiprid, d-tetramethrin, and piperonyl butoxide (d.a.p.) have never been examined for sublethal effects on <em>A. diaperinus</em> locomotion. Thus, the present study evaluated the impact of their LC<sub>10</sub> and LC<sub>30</sub> in the mobility traits of <em>A. diaperinus</em> adults (30 individuals/treatment), in the absence and presence of food source, after 8, 12, 24, and 48 h of exposure. Deltamethrin was more toxic compared to d.a.p. ANOVA revealed significant disruptions in locomotion, including increased immobility, reduced walking speed, and diminished climbing ability, especially with the LC<sub>30</sub> of deltamethrin, compared to the control group. The longest walking period was observed with the 12-h control group (609.5 s) vs. LC<sub>10</sub> and LC<sub>30</sub> of deltamethrin (235.3 and 300.2 s, respectively) and LC<sub>10</sub> and LC<sub>30</sub> of d.a.p. (456.1 and 481.7 s, respectively). Walking speed in the control groups was >46.0 mm/s while decreased to 7.9 and 8.8 mm/s after 48 h of exposure to the LC<sub>30</sub> of deltamethrin and d.a.p., respectively. Negative effects were noted for the mobility of <em>A. diaperinus</em> in the presence of food source. Control group individuals stayed in the food for >174 s while the insecticides-treated individuals for <86 s at 12, 24, and 48 h of exposure. Both insecticides negatively influenced the mobility behavior in the presence and absence of food source and the walking speed. These findings underscore the potential of considering sublethal concentrations of insecticides in integrated pest management strategies.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107327"},"PeriodicalIF":2.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Neopestalotiopsis rhododendri causing dragon fruit (Selenicereus monacanthus) stem spot in China 中国火龙果（Selenicereus monacanthus）茎斑病新直立多毛opsis rhododendri的首次报道

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-29 DOI: 10.1016/j.cropro.2025.107326

Qing Guo , Hong Zhang , Kexin Cheng , Kunmin Yu , Yulan Jiang

引用次数: 0

Morphological changes and spray coverage of pear leaves and canopy at different phenological periods during air-assisted spraying 空气助喷不同物候期梨树叶片和冠层形态变化及喷淋盖度

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-27 DOI: 10.1016/j.cropro.2025.107324

Jian Zhang , Qing Chen , Hongping Zhou , Can Liu , Runtao Han , Xiaolan Lv

{"title":"Morphological changes and spray coverage of pear leaves and canopy at different phenological periods during air-assisted spraying","authors":"Jian Zhang , Qing Chen , Hongping Zhou , Can Liu , Runtao Han , Xiaolan Lv","doi":"10.1016/j.cropro.2025.107324","DOIUrl":"10.1016/j.cropro.2025.107324","url":null,"abstract":"<div><div>The airflow of air-assisted spraying in orchards can effectively promote the deformation of leaves and canopies, broaden the droplet transport channels, and improve the uniform of droplet deposition. In this study, the single pear leaf and tree canopy were treated as research objects for micro and macro analyses. The morphological changes and spray coverage of leaves and canopies at the young fruit stage and fruit expansion stage were investigated from the micro and macro perspectives through wind tunnel experiments. Two high-speed cameras were utilized to record the morphological changes of pear leaves and canopies under wind speeds and the multi-point anemometer was used to measure the wind speed attenuation within the canopy. The image processing method was used to obtain the leaf windward area and spray coverage, canopy windward area, optical porosity and spray coverage. The experiments showed that the morphological changes and spray coverage of single leaves and canopies were moderately consistent. The leaf elastic modulus under different phenological periods affected the morphological changes of leaf and canopy, which together with the leaf surface contact angle affected the spray coverage. Within the experimental parameters, the highest total spray coverage in the pear canopy was observed at a wind speed of 6 m s<sup>−1</sup> and the spray application was reduced by approximately 36.96 %. In the orchard air-assisted spraying, considering the spray coverage of single leaf and canopy, it is recommended that the wind speed for pear trees at young fruit stage and fruit expansion stage are 3.5–6 m s<sup>−1</sup> and 5–7 m s<sup>−1</sup>, respectively. This study proposes to adopt various spraying parameters at different phenological periods, which can provide a reference for the adjustment of spraying parameters and the optimization of spraying system.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107324"},"PeriodicalIF":2.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Lithocarpus henryi leaf blight caused by Colletotrichum siamense in China 由炭疽病菌引起的立木叶枯病在中国首次报道

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-23 DOI: 10.1016/j.cropro.2025.107319

Yuxuan Huang, Shaohua Huang, Jia Cao, Qian Li, Linping Zhang

引用次数: 0

An overview of insecticide resistance mechanisms, challenges, and management strategies in Spodoptera frugiperda 夜蛾抗药性机制、挑战及防治策略综述

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-23 DOI: 10.1016/j.cropro.2025.107322

Patrick Maada Ngegba , Muhammad Zaryab Khalid , Wayne Jiang , Guohua Zhong

{"title":"An overview of insecticide resistance mechanisms, challenges, and management strategies in Spodoptera frugiperda","authors":"Patrick Maada Ngegba , Muhammad Zaryab Khalid , Wayne Jiang , Guohua Zhong","doi":"10.1016/j.cropro.2025.107322","DOIUrl":"10.1016/j.cropro.2025.107322","url":null,"abstract":"<div><div>The fall armyworm, <em>Spodoptera frugiperda</em>, has become a significant threat to global agriculture, particularly in sub-Saharan Africa (SSA). Numerous reports indicate substantial insecticide resistance to pyrethroids and organophosphates in <em>S. frugiperda</em> populations. Given the existential and rampant resistance to key conventional insecticides, a new paradigm shift in insecticide resistance management (IRM) of <em>S. frugiperda</em> is urgently needed. This necessitates a critical review of key mechanisms underlying pest biology, ecology, and behavior alongside an evaluation of current insecticide application techniques, pest management practices, and policy frameworks. Notably, the existing literature underscores <em>S. frugiperda's</em> high propensity to develop resistance to various synthetic insecticides. Therefore, an in-depth understanding of the mechanisms underlying resistance is essential for developing sustainable and effective resistance management programs that aligned with the Integrated Pest Management (IPM) scheme. This review discusses the <em>S. frugiperda</em> resistance menace, mechanisms involved, and sustainable management techniques that will assist key stakeholders in making an informed policy formulation to safeguard the pest management subsector and enhance agricultural productivity and food security.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107322"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing control of Stemphylium vesicarium on asparagus fern using TOMCAST and new fungicides 利用TOMCAST和新型杀菌剂防治芦笋茎蚜的研究进展

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-22 DOI: 10.1016/j.cropro.2025.107321

John R. Spafford, Mary K. Hausbeck

{"title":"Advancing control of Stemphylium vesicarium on asparagus fern using TOMCAST and new fungicides","authors":"John R. Spafford, Mary K. Hausbeck","doi":"10.1016/j.cropro.2025.107321","DOIUrl":"10.1016/j.cropro.2025.107321","url":null,"abstract":"<div><div><em>Stemphylium vesicarium</em>, the causal agent of purple spot disease on asparagus, renders spears unmarketable and causes premature defoliation of the fern, reducing subsequent spear yield. The fungicides azoxystrobin, mancozeb, and chlorothalonil are registered to control purple spot on the fern and may be applied according to the TOMCAST disease forecaster or a calendar-based schedule. Registered fungicides were compared to the non-registered fungicides pydiflumetofen + fludioxonil and fluxapyroxad + pyraclostrobin, each alternated with chlorothalonil, and were applied according to TOMCAST at a threshold of 15 or 20 disease severity values (DSV) or every 10 days or not treated (control). The 10-day treatment received eight applications each year, TOMCAST 15 DSV received eight (2022) or six (2023); TOMCAST 20 DSV treatment received six (2022) or four (2023). Mancozeb applied every 10 days (2023 only) or azoxystrobin applied every 10 days or according to TOMCAST 15 DSV had less disease than the control at the final assessment. Pydiflumetofen + fludioxonil and fluxapyroxad + pyraclostrobin had less disease than the control at the final assessment at all intervals. Relative area under the disease progress curve (rAUDPC) data indicated that all treatments limited purple spot disease compared to the control in 2022 and 2023 except for mancozeb applied at TOMCAST 20 DSV (2022). The rAUDPC data were similar at all application timings for pydiflumetofen + fludioxonil and fluxapyroxad + pyraclostrobin each year. Results indicate TOMCAST 20 DSV can be used with locally systemic, highly efficacious, fungicides to optimize spray applications to protect asparagus fern.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107321"},"PeriodicalIF":2.5,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current status of herbicide resistance in global cotton production: trends and perspectives 全球棉花生产中除草剂抗性现状：趋势与展望

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-21 DOI: 10.1016/j.cropro.2025.107320

Christos A. Damalas, Spyridon D. Koutroubas

{"title":"Current status of herbicide resistance in global cotton production: trends and perspectives","authors":"Christos A. Damalas, Spyridon D. Koutroubas","doi":"10.1016/j.cropro.2025.107320","DOIUrl":"10.1016/j.cropro.2025.107320","url":null,"abstract":"<div><div>The evolution of herbicide innovations continues to deliver benefits to cotton growers, but the threat of resistant weeds with repeated application of the same active ingredients has been steadily increasing over the last 20 years. In this study, a critical overview of herbicide resistance cases in cotton production globally is reported following data retrieved from the International Herbicide-Resistant Weed Database and the scholarly literature. The first case of herbicide resistance in cotton dates back to 1973, referring to trifluralin-resistant <em>Eleusine indica</em> in the United States. Since then, one hundred and nineteen (119) cases refer to resistant weeds in cotton during the period from 1973 to 2020. More than three-fourths of the resistant-weed cases (76.5 %) in cotton come from the United States. The cases increased dramatically after 2000. The most frequently reported broadleaf weeds with herbicide resistance are <em>Amaranthus palmeri</em>, <em>Amaranthus tuberculatus</em>, and <em>Xanthium strumarium</em>. The most frequently reported grasses with herbicide resistance are <em>Eleusine indica</em> and <em>Sorghum halepense</em>. Most cases of herbicide resistance refer to glyphosate (HRAC Group-9) and ALS inhibitors (HRAC Group-2), followed by microtubule assembly inhibitors (HRAC Group-3) and ACCase inhibitors (HRAC Group-1). To ensure the value of herbicides, growers must change weed control towards a more sustainable way, re-implementing an integrated approach to weed control with rotation of herbicide groups as a key principle for reducing herbicide resistance. Moreover, residual herbicides should be backed up with additional practices, such as tillage, cultivation plus chipping, and knockdown herbicides, other than glyphosate.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107320"},"PeriodicalIF":2.5,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenerational Sublethal Effects of sulfoxaflor on the Suppression of Aphis citricola Van der Goot (Hemiptera: Aphididae) 亚砜氟对柑桔蚜的跨代亚致死效应（半翅目：蚜科）

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-18 DOI: 10.1016/j.cropro.2025.107318

Yuntao Feng, Xiaojun Guo, Ya Li, Yameng Zhang, Kang Jia, Runxiang Zhang

{"title":"Transgenerational Sublethal Effects of sulfoxaflor on the Suppression of Aphis citricola Van der Goot (Hemiptera: Aphididae)","authors":"Yuntao Feng, Xiaojun Guo, Ya Li, Yameng Zhang, Kang Jia, Runxiang Zhang","doi":"10.1016/j.cropro.2025.107318","DOIUrl":"10.1016/j.cropro.2025.107318","url":null,"abstract":"<div><div>The spirea aphid <em>Aphis citricola</em> Van der Goot is a serious fruit trees pest in China. Sulfoxaflor is a novel sulfoximine insecticide that has been widely used to control sucking insect pests. This study aimed to investigate the transgenerational sublethal effects of sulfoxaflor on the biological traits and metabolic enzymes activities of <em>A. citricola</em>. Our results showed that sublethal concentrations of sulfoxaflor (LC<sub>10</sub> and LC<sub>20</sub>) significantly reduced the adult longevity and fecundity of the F<sub>0</sub> generation. Furthermore, the development time of the first instar nymph and the pre-adult of F<sub>1</sub> generation were significantly prolonged. The total prereproductive period (TPRP) and the mean generation time (<em>T</em>) of the F<sub>1</sub> generation were also significantly extended. Consequently, the fecundity, intrinsic rate of increase (<em>r</em><sub><em>m</em></sub>), and finite rate of increase (<em>λ</em>) of the F<sub>1</sub> generation were significantly diminished. Additionally, the activities of cytochrome P450 monooxygenases (P450) and carboxylesterase (CarE) in <em>A. citricola</em> were enhanced following treatment with sublethal concentrations of sulfoxaflor, indicating their involvement in the detoxification of sulfoxaflor. These results indicate that sublethal concentrations of sulfoxaflor inhibited the development and reproduction of <em>A. citricola</em> in both the exposed generation and the subsequent generation, leading to a suppression of population growth over two consecutive generations. This research provides a foundation for a deeper understanding of the intergenerational sublethal effects of sulfoxaflor and suggests that sulfoxaflor has promising potential for controlling <em>A. citricola</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107318"},"PeriodicalIF":2.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of potato aucuba mosaic virus 一步反转录环介导等温扩增（RT-LAMP）检测马铃薯澳瓜花叶病毒的方法建立

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-15 DOI: 10.1016/j.cropro.2025.107317

An Yan, Yunting Lei, Zhaoyao Yu, Gaoli Wu, Wenzhuo Feng, Xiangru Chen

{"title":"Establishment of one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of potato aucuba mosaic virus","authors":"An Yan, Yunting Lei, Zhaoyao Yu, Gaoli Wu, Wenzhuo Feng, Xiangru Chen","doi":"10.1016/j.cropro.2025.107317","DOIUrl":"10.1016/j.cropro.2025.107317","url":null,"abstract":"<div><div>Potatoes, as the fourth largest global food crop, are extensively cultivated worldwide. However, potato production is significantly impacted by plant viruses, among which the potato aucuba mosaic virus (PAMV) is a notable threat. PAMV frequently co-infects with other plant viruses, severely reducing potato yields and posing a risk to the sustainable development of the potato industry. To better control the disease caused by PAMV, a practical detection method is urgently in need. In this study, we developed a one-step reverse-transcription loop-mediated isothermal amplification (one-step RT-LAMP) assay for the detection of PAMV. Primers for the one-step RT-LAMP were designed by targeting conserved regions of all PAMV isolates available in online databases, and the reaction conditions, including temperature, were optimized. Through comparative analysis with conventional RT-PCR and serological tests, we confirmed that the one-step RT-LAMP method developed here is both specific and efficient. Furthermore, the detection limit of the assay was determined to be approximately 2 copies of PAMV, demonstrating its high sensitivity. The practical applicability of the method was validated by testing potato and pepino samples collected from Gansu and Yunnan provinces, with results indicating its effectiveness in field sample detection. Overall, our findings demonstrate that the one-step RT-LAMP method is accurate, reliable, and highly practical, offering a valuable tool for controlling PAMV derived diseases and contributing to the sustainable development of the potato industry.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107317"},"PeriodicalIF":2.5,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric one-step reverse transcription loop-mediated isothermal amplification for rapid and specific detection of sunflower chlorotic mottle virus in sunflower 比色法一步反转录环介导等温扩增快速特异检测向日葵变色斑驳病毒

IF 2.5 2区农林科学

Crop Protection Pub Date : 2025-06-15 DOI: 10.1016/j.cropro.2025.107316

Salit Supakitthanakorn, Kanungnit Reanwarakorn

{"title":"Colorimetric one-step reverse transcription loop-mediated isothermal amplification for rapid and specific detection of sunflower chlorotic mottle virus in sunflower","authors":"Salit Supakitthanakorn, Kanungnit Reanwarakorn","doi":"10.1016/j.cropro.2025.107316","DOIUrl":"10.1016/j.cropro.2025.107316","url":null,"abstract":"<div><div>Sunflower chlorotic mottle virus (SuCMoV), an emerging sunflower-infecting virus in Thailand, was considered as one of the most frequently emerged viruses infecting sunflowers causing chlorotic spots, mottle, yellowing and stunting symptoms. This study aimed to develop and validate the colorimetric one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of SuCMoV in sunflowers. We optimized the RT-LAMP conditions and determined that incubation at 65 °C for 30 min in a heat block incubator provided optimal results, as confirmed by the presence of ladder-like pattern LAMP products visualized through agarose gel electrophoresis. In the colorimetric detection system, positive reactions were clearly indicated by a color change from pink to yellow, while negative reactions remained pink. The specificity test verified that the LAMP primers did not cross-react with other potyviruses. Sensitivity analysis demonstrated that the RT-LAMP assay could detect SuCMoV RNA at concentrations as low as 10 fg/μL which were greater than those of one-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) and one-step RT-PCR. Validation of the colorimetric one-step RT-LAMP assay using naturally infected sunflower samples confirmed its ability to successfully detect SuCMoV, even in mixed viral infections with closely related sunflower-infecting potyviruses detecting by multiplex RT-PCR. To our knowledge, this study represents the first development of a colorimetric one-step RT-LAMP assay for rapid and sensitive detection of SuCMoV.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"197 ","pages":"Article 107316"},"PeriodicalIF":2.5,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0