Wild sources for host plant resistance to Bemisia tabaci in watermelon: insights from behavioral and chemical analyses

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-09-19 DOI:10.1016/j.cropro.2025.107411

Petrina McKenzie-Reynolds , Isiaka A. Owolabi , Anju Biswas , Quentin D. Read , Gaelen R. Burke , Amnon Levi , Alvin M. Simmons

{"title":"Wild sources for host plant resistance to Bemisia tabaci in watermelon: insights from behavioral and chemical analyses","authors":"Petrina McKenzie-Reynolds , Isiaka A. Owolabi , Anju Biswas , Quentin D. Read , Gaelen R. Burke , Amnon Levi , Alvin M. Simmons","doi":"10.1016/j.cropro.2025.107411","DOIUrl":null,"url":null,"abstract":"<div><div>Whitefly infestations, primarily due to <em>Bemisia tabaci</em> (Gennadius) (Hemiptera: Aleyrodidae), pose a serious threat to watermelon production, leading to significant yield losses and increased reliance on chemical pesticides. In this study, genotypes of the desert-watermelon <em>Citrullus colocynthis</em> (L.) Schrader and the cultivated watermelon (<em>C</em>. <em>lanatus</em> (Thunberg) Matsumura & Nakai) were evaluated for resistance to <em>B. tabaci</em> using oviposition and vertical Y-tube olfactometer assays. In addition, gas chromatography-mass spectrometry (GC-MS) analysis of plant volatiles was conducted. Among 13 wild accessions tested, two received statistically less oviposition, while all wild genotypes demonstrated statistically reduced attraction of adult whiteflies to plant volatiles as compared with a susceptible cultivar. GC-MS analysis identified phenol derivatives and sesquiterpenes as dominant volatiles in the <em>C. colocynthis</em> resistant accessions. The volatile blend of the wild United States Plant Introduction (PI)-542616 was dominated by phenolic derivatives, comprising nearly the entire profile, while PI-386019 released a greater proportion of antioxidant-rich phenolic esters. These differences highlight clear variation in the relative abundance of major chemical classes among accessions of <em>C. colocynthis</em>, which may contribute to differential resistance against <em>B. tabaci</em>. These findings help elucidate the biochemical and volatile-mediated whitefly-resistance mechanisms in <em>C. colocynthis</em>. By integrating resistant germplasm into integrated pest management (IPM) strategies, results from this research will advance sustainable watermelon production.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"199 ","pages":"Article 107411"},"PeriodicalIF":2.5000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0261219425003035","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Whitefly infestations, primarily due to Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), pose a serious threat to watermelon production, leading to significant yield losses and increased reliance on chemical pesticides. In this study, genotypes of the desert-watermelon Citrullus colocynthis (L.) Schrader and the cultivated watermelon (C. lanatus (Thunberg) Matsumura & Nakai) were evaluated for resistance to B. tabaci using oviposition and vertical Y-tube olfactometer assays. In addition, gas chromatography-mass spectrometry (GC-MS) analysis of plant volatiles was conducted. Among 13 wild accessions tested, two received statistically less oviposition, while all wild genotypes demonstrated statistically reduced attraction of adult whiteflies to plant volatiles as compared with a susceptible cultivar. GC-MS analysis identified phenol derivatives and sesquiterpenes as dominant volatiles in the C. colocynthis resistant accessions. The volatile blend of the wild United States Plant Introduction (PI)-542616 was dominated by phenolic derivatives, comprising nearly the entire profile, while PI-386019 released a greater proportion of antioxidant-rich phenolic esters. These differences highlight clear variation in the relative abundance of major chemical classes among accessions of C. colocynthis, which may contribute to differential resistance against B. tabaci. These findings help elucidate the biochemical and volatile-mediated whitefly-resistance mechanisms in C. colocynthis. By integrating resistant germplasm into integrated pest management (IPM) strategies, results from this research will advance sustainable watermelon production.

查看原文本刊更多论文

西瓜寄主植物抗烟粉虱的野生来源：来自行为和化学分析的见解

主要由烟粉虱（半翅目：烟粉虱科）引起的粉虱侵染对西瓜生产构成严重威胁，导致严重的产量损失和对化学农药的依赖增加。本研究对荒漠西瓜Citrullus colocynthis （L.）的基因型进行了分析。采用产卵法和垂直y管嗅觉法对施拉德和栽培西瓜（C. lanatus (Thunberg) Matsumura & Nakai）对烟粉虱的抗性进行了评价。此外，还对植物挥发物进行气相色谱-质谱（GC-MS）分析。在13个野生品种中，有2个品种的产卵量明显减少，而所有野生基因型的白蝇对植物挥发物的吸引力均明显低于敏感品种。气相色谱-质谱分析表明，酚类衍生物和倍半萜类化合物是油菜耐药种质的主要挥发物。野生美国植物引种(PI)-542616的挥发性混合物以酚类衍生物为主，几乎包含了整个剖面，而PI-386019释放出更多的富含抗氧化剂的酚类酯。这些差异凸显了不同种质间主要化学类相对丰度的明显差异，这可能是造成对烟粉虱抗性差异的原因之一。这些发现有助于阐明绿草甘蓝抗白蝇的生化和挥发物介导的机制。通过将抗性种质整合到害虫综合治理（IPM）策略中，本研究结果将促进西瓜的可持续生产。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.