Crop Protection最新文献

{"title":"Detection of Diaporthe cinerascens from latent and symptomatic fig tree cankers using species-specific primers","authors":"Zeinab Bolboli,&nbsp;Hamed Negahban,&nbsp;Reza Mostowfizadeh-Ghalamfarsa","doi":"10.1016/j.cropro.2025.107211","DOIUrl":"10.1016/j.cropro.2025.107211","url":null,"abstract":"<div><div>Fig canker is a multi-agent and complex disease, and since its identification, Diaporthe canker, primarily caused by <em>Diaporthe cinerascens</em>, has emerged as one of the most persistent and destructive diseases affecting fig trees in regions such as Iran, California, Canada, and Italy. The economic implications of this disease are considerable, further aggravated by the lack of effective therapeutic interventions. This study presents the development and validation of novel species-specific PCR primers designed to enhance the early and accurate detection of Diaporthe canker in artificially and naturally infected samples with mixed and latent infections. We utilized pure cultures of well-characterized <em>D. cinerascens</em> isolates, whose proven pathogenicity and genetic homogeneity were validated in earlier studies. Among the 82 developed primers, the TUB-Dc1 and HIS-Dc1 pairs were chosen after a thorough analysis of the β-tubulin (<em>tub</em>) and histone 3 (<em>his3</em>) genes, along with the internal transcribed spacer of rDNA (ITS) region. These selected primers successfully amplified target gene sequences in pure cultures and infected tissue samples using direct and nested PCR. The diagnostic sensitivity thresholds achieved were 100 fg μL⁻¹ for direct PCR and 1 ag μL⁻¹ for nested PCR. Notably, nested PCR significantly enhanced detection capabilities by up to 100,000-fold compared to direct PCR. The TUB-Dc1 primer pair effectively identified <em>D. cinerascens</em> in 80 % of samples with Type A canker, 22.7 % with Type C canker, and 8.8 % showing dieback symptoms. In comparison, conventional cultural methods detected the pathogen in only 30 % of Type A canker samples, and 4.4 % of Type C canker samples, with no detections in samples showing dieback. Furthermore, our results underscore the potential utility of our molecular tools for precise identification and detecting latent infections in asymptomatic samples via nested PCR. Our findings indicate that the species-specific assays are highly sensitive, specific, and reliable for detecting <em>D. cinerascens</em> in symptomatic and asymptomatic environmental samples. Additionally, this tool paves the way for future applications aimed at certifying pathogen-free fig saplings, an essential component for sustainable fig cultivation practices.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107211"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential parasitism performance and host adaptability of key biocontrol Eretmocerus species against two major whitefly pests","authors":"Xi-Ya Wang ,&nbsp;Jia-Wei Sun ,&nbsp;Lucie S. Monticelli ,&nbsp;Nicolas Desneux ,&nbsp;Lian-Sheng Zang","doi":"10.1016/j.cropro.2025.107212","DOIUrl":"10.1016/j.cropro.2025.107212","url":null,"abstract":"<div><div>Whiteflies are major agricultural pests, and their sustainable management depends on effective biological control strategies. This study investigates the preference, the host performance and the host feeding behavior of two key parasitoid species, <em>Eretmocerus corni</em> and <em>Eretmocerus hayati</em>, against <em>Bemisia tabaci</em> and <em>Trialeurodes vaporariorum</em>. To assess host selection, parasitism, host-feeding, emergence rates, developmental times, proportion of female offspring, and total mortality rate across different host species and instars, both no-choice and choice experiments were conducted. Key findings include: (1) <em>E. corni</em> successfully parasitized all instars of both whitefly species, while <em>E. hayati</em> completed its development only in <em>B. tabaci</em>. Both parasitoids achieved the highest parasitism rates and emergence rates on the second and third instars of their respective hosts. (2) <em>E. hayati</em> exhibited superior host-feeding, faster development, and higher total mortality rate compared to <em>E. corni</em>, additionally, both host-feeding rates and developmental time significantly decreased as the host instar increased. (3) Although <em>E. hayati</em> could not complete its development in <em>T. vaporariorum</em>, it caused substantial mortality through aborted parasitism and superior host-feeding. (4) In the choice tests, <em>E. corni</em> showed a significant preference for <em>T. vaporariorum</em> when both whitefly species were at the same instar stage, whereas <em>E. hayati</em> exhibited no notable host preference. (5) Under the mixed-host condition, both <em>E. corni</em> and <em>E. hayati</em> exhibited increased mortality rates against whiteflies at the same instar compared to the single-host condition. These findings offer valuable insights into the host preference, performance of these parasitoids and provide theoretical guidance for optimizing whitefly biological control.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107212"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbicide selectivity and efficacy in weed control in canola production system","authors":"Natalia Costa ,&nbsp;Guilherme Vieira Pimentel ,&nbsp;Amanda Santana Chales ,&nbsp;Luiz Daniel Rodrigues da Silva ,&nbsp;Elisabete da Cruz Silva Watanabe ,&nbsp;Adenilson Henrique Gonçalves","doi":"10.1016/j.cropro.2025.107207","DOIUrl":"10.1016/j.cropro.2025.107207","url":null,"abstract":"<div><div>Canola is an essential oilseed in global agriculture, contributing to the economic, social, and environmental sectors due to its versatility and nutritional value. Despite the notable growth of canola in Brazil, weed management poses a challenge due to the scarcity of registered selective herbicides and the prohibition of transgenics, resulting in productivity losses. Therefore, it is crucial to develop innovative technologies and generate knowledge to guide effective weed management and facilitate the expansion of canola in the country. Field studies during the 2022/2023 growing season evaluated the selectivity of herbicides in canola production and their effectiveness in weed control. The pre-emergent treatments were: clomazone, s-metolachlor, clomazone + s-metolachlor, and no pre-emergent herbicide; and the post-emergent treatments were: quinclorac, dicamba, quinclorac + dicamba, and no post-emergent herbicide, in addition to a weed-free control. Although clomazone, quinc lorac, and dicamba are not registered for use in canola in Brazil, they showed promising results by significantly reducing weed interference without negatively impacting oil and protein contents or yield components. Combinations S-metolachlor + clomazone and quinclorac + dicamba, provided the best outcomes in both weed control and grain productivity, with yields reaching 2053.29 kg ha⁻¹ in Lavras. These findings suggest that the tested herbicides have potential for selective use in canola production, paving the way for further studies aimed at their registration and incorporation into production systems.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107207"},"PeriodicalIF":2.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance of ten Indonesian cassava cultivars to the cassava lacebug, Vatiga illudens (Drake) (Hemiptera: Tingidae), a newly invasive exotic species in Asia","authors":"Retno Dyah Puspitarini,&nbsp;Ave Maria Simbolon,&nbsp;Chairunisah Putri,&nbsp;Hendy Dwi Bayu Ardianto,&nbsp;Vriescha Ermalitha Ramadhanti Rudiantoyo,&nbsp;Windi Puspa Saputri,&nbsp;Faiz Nashiruddin Muhammad,&nbsp;Ito Fernando","doi":"10.1016/j.cropro.2025.107208","DOIUrl":"10.1016/j.cropro.2025.107208","url":null,"abstract":"<div><div>The cassava lacebug, <em>Vatiga illudens</em> (Drake) (Hemiptera: Tingidae), is a pest that feeds on cassava foliage, significantly reducing the yield of tuberous roots. Recently introduced to Asia, this invasive exotic pest has been reported only in Indonesia, where it poses a serious threat to cassava cultivation. This study aimed to determine the resistance levels of ten Indonesian cassava cultivars to <em>V. illudens</em>. The leaf nutritional profile (proximate content), as well as phenol and hydrogen cyanide (HCN) content, were analyzed as potential factors affecting the biological parameters of <em>V. illudens</em>. Results from the choice test revealed that <em>V. illudens</em> adults exhibited a clear preference for certain cultivars, with the highest numbers observed on Gajah (10.00 individuals), Adira 1 (5.60 individuals), and Mentega (5.20 individuals). In contrast, adult numbers on the remaining cultivars were lower, ranging from 2.40 to 3.60 individuals. In the no-choice antibiosis test, the nymphal developmental period (from first instar to adult) ranged from 11.70 to 20.40 days, while adult longevity varied from 9.05 to 16.30 days. Leaf HCN content was the only chemical property found to be associated with the degree of resistance in cassava cultivars, with low-HCN cultivars like Gajah (9.66 mg/g) and Mentega (11.26 mg/g) being more preferred by <em>V. illudens</em>. The insect also exhibited faster development and extended adult longevity on these cultivars, leading to increased population growth. These findings are expected to provide valuable information for the management of <em>V. illudens</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107208"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo compatibility of isocycloseram-based insecticides with entomopathogenic fungi used for corn leafhopper management","authors":"Maria Eduarda Sperotto ,&nbsp;Matheus Rakes ,&nbsp;Maíra Chagas Morais ,&nbsp;Daniel Bernardi ,&nbsp;Leandro do Prado Ribeiro","doi":"10.1016/j.cropro.2025.107204","DOIUrl":"10.1016/j.cropro.2025.107204","url":null,"abstract":"<div><div>Recently registered, isocycloseram-based insecticides have the corn leafhopper (<em>Dalbulus maidis</em>) as a main target in Brazil. Nevertheless, the management of this vector is complex and requires the integration of strategies, including chemical and fungal agents. Here, we investigated the compatibility of mycoinsecticides based on isolate Esalq-1296 of <em>Cordyceps javanica</em> and Simbi BB15 of <em>Beauveria bassiana</em> with isocycloseram-based insecticides and their mixtures. <em>In vitro</em>, the biological index adopted indicated that the insecticide based on isocycloseram + lambda-cyhalothrin (at 125- and 250-mL ha⁻¹) was classified as moderately compatible with the Esalq-1296 isolate of <em>C. javanica</em> and Simbi BB15 of <em>B. bassiana</em>. The isocycloseram-based formulation was classified as compatible with the Simbi BB15 isolate of <em>B. bassiana</em>, at both doses tested, and moderate compatible and compatible with the Esalq-1296 isolate of <em>C. javanica</em> at 100 and 50 mL ha⁻¹, respectively. Further analysis revealed that the majority of insecticide-mycoinsecticides mixtures exhibited physicochemical compatibility, with the exception of the mixture comprising these insecticides and the mycoinsecticide-based on <em>B. bassiana</em> (FlyControl®), which demonstrated an absence of homogeneity. The <em>in vivo</em> bioassays demonstrated an additive effect on leafhopper mortality and an increase in the velocity of lethal toxicity when these insecticides (at LC₃₀) were combined with the tested mycoinsecticides. However, the insecticides reduced the extrusion rate and sporulation from dead leafhoppers. As these parameters are crucial for fungal persistence by establishing a secondary cycle of epizootics in cornfields, caution is advised when combining these chemical and fungal agents for the management of <em>D. maidis</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"194 ","pages":"Article 107204"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum “Cyantraniliprole-induced intergenerational sublethal effects on fall armyworm, Spodoptera frugiperda” [Crop Protect. 190 107116 https://doi.org/10.1016/j.cropro.2025.107116]","authors":"Arzlan Abbas ,&nbsp;Ali Hasnain ,&nbsp;Faisal Hafeez ,&nbsp;Wu Hai Chao ,&nbsp;Dong Yu Hua ,&nbsp;Aleena Alam ,&nbsp;Sohail Abbas ,&nbsp;Ayesha Iftikhar ,&nbsp;Hamed A. Ghramh ,&nbsp;Khalid Ali Khan ,&nbsp;Farman Ullah ,&nbsp;Chen Ri Zhao","doi":"10.1016/j.cropro.2025.107199","DOIUrl":"10.1016/j.cropro.2025.107199","url":null,"abstract":"","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107199"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of termination timing and incorporation of winter cover crop on the suppression of plant-parasitic nematodes and weeds","authors":"Denis Gitonga ,&nbsp;Xiaoying Li ,&nbsp;Abolfazl Hajihassani","doi":"10.1016/j.cropro.2025.107205","DOIUrl":"10.1016/j.cropro.2025.107205","url":null,"abstract":"<div><div>Organic vegetable production faces challenges in managing plant-parasitic nematodes (PPNs) and weeds, as chemical options are prohibited. This study evaluated the effects of cover crop termination timing (90 and 120 days after planting, DAP) and cover crop incorporation, along with the application of an organic bioherbicide (Suppress EC), on PPN and weed suppression during the 2021 and 2022 winter seasons. The experiment utilized seven treatments of oilseed radish, oat, rye, mustard, a rye-oat mixture, and two fallows (with and without weeds), replicated five times per season for each termination timing and terminated at 90 and 120 DAP and incorporated into the soil. Results showed that termination timing significantly influenced PPN populations, with rye and oat reducing <em>Meloidogyne incognita</em> densities when terminated at 90 DAP, while mustard and radish increased nematode populations at 120 DAP. Incorporation of cover crops did not consistently enhance nematode suppression, especially in low-biomass scenarios. Weed suppression was more effective with later termination (120 DAP), particularly for rye and oat, which reduced weed biomass through shading and allelopathic effects. The combination of cover crops with bioherbicide further improved weed control, with significant reductions in corn spurry (<em>Spergula arvensis</em>) and cutleaf geranium (<em>Geranium dissectum</em>) compared to cover crops alone. However, the bioherbicide was less effective in suppressing weeds in mustard and radish plots. These findings highlight the importance of selecting appropriate cover crops and optimizing termination timing to manage PPNs and weeds. Integrating bioherbicides with cover crops offers a promising strategy for organic vegetable production, enhancing weed suppression and contributing to sustainable agroecosystems.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107205"},"PeriodicalIF":2.5,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of Diaporthe phoenicicola causing leaf spot on Rosa roxburghii in China","authors":"Junxian Chen ,&nbsp;Liangyu Xiao ,&nbsp;Taotao An ,&nbsp;Wei Jia ,&nbsp;Qinyi Hu ,&nbsp;Ying Xu ,&nbsp;Zhu Li ,&nbsp;Haixia Ding","doi":"10.1016/j.cropro.2025.107194","DOIUrl":"10.1016/j.cropro.2025.107194","url":null,"abstract":"<div><div><em>Rosa roxburghii</em> Tratt is commonly found growing wild in the south China. In recent years, it has been extensively cultivated in Guizhou, Sichuan, and Chongqing. The fruit of <em>Rosa roxburghii</em> Tratt serves as a valuable resource for both medicinal and culinary purposes, with a long-standing history of use in China. Recently, a new leaf spot was found in the field survey of <em>R. roxburghii</em> in Guizhou Province. The pathogens were isolated and identified by conventional isolation methods, and three genes internal transcriptional spacer (ITS), β-microtubulin (TUB2) and translation elongation factor 1-a (EF1-a) were analyzed. Based on morphological characteristics and phylogenetic analyses, the pathogenic fungus was identified as <em>Diaporthe phoenicicola</em>. Pathogenicity test was confirmed by inoculating <em>R. roxburghii</em> cultivars under greenhouse conditions. All <em>R. roxburghii</em> plants inoculated with <em>D.phoenicicola</em> developed symptoms identical to those observed on naturally infected plants in the field. The fungus were reisolated from the diseased leaves and identified as <em>D.phoenicicola</em> based on its morphological and molecular characteristics, confirming Koch's postulates. To our knowledge, this is the first report of <em>R</em>. <em>roxburghii</em> leaf spot disease caused by <em>D.phoenicicola</em> in China. After being infected by leaf spot disease, the <em>R. roxburghii</em> will develop brown or black spots, which may weaken photosynthesis, cause premature leaf senescence and shedding, and reduce the plant's stress resistance. Therefore, this disease may become a threat to <em>R</em>. <em>roxburghii</em> production in China.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107194"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-component image analysis for citrus disease detection using convolutional neural networks","authors":"Parul Sharma ,&nbsp;Pawanesh Abrol","doi":"10.1016/j.cropro.2025.107181","DOIUrl":"10.1016/j.cropro.2025.107181","url":null,"abstract":"<div><div>Citrus crops are susceptible to diseases such as Black Spot, Canker, and Greening, which significantly harm both the leaves and the fruits, ultimately reducing overall yield. Traditional visual inspection methods for identifying these diseases are labour-intensive and prone to inaccuracies. The present research proposes a deep learning approach utilizing Convolutional Neural Networks (CNNs) to overcome the limitations of manual inspection. Moreover, it introduces the utilization of combined visual features of citrus leaves and fruits for enhanced disease classification. The proposed multi-component approach demonstrates superior classification performance, achieving more accurate results than single-component-based classifications.</div><div>A dataset comprising 12,000 images, distributed across leaves, fruits, and their merged forms, was used for training, validation, and testing. Three CNN models were developed and evaluated: Leaf-Trained, Fruit-Trained, and Multiple Component-Trained CNNs. Performance was assessed using metrics such as accuracy, precision, recall, and F1-score, including their macro values, focusing on model generalization across different input types. The Multiple Component-Trained CNN outperformed the other models, achieving a validation accuracy of 97.75%, followed by the Leaf-Trained CNN at 95.50%. During testing, it also demonstrated superior performance across all input types, with accuracies of 94.75% on the leaf dataset, 92.87% on the fruit dataset, and 96.62% on the merged dataset. The results indicate that Black Spot is the most accurately classified disease, while Canker and Greening are less accurately classified. These findings highlight the potential of integrating various components of plants for enhanced disease classifications.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107181"},"PeriodicalIF":2.5,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of leaf blight caused by Cladosporium cladosporioides on Fagopyrum dibotrys in China","authors":"Xiaoyan Huang ,&nbsp;Xixu Peng ,&nbsp;Huan Xie ,&nbsp;Limei Liao ,&nbsp;Yanyu Zhou ,&nbsp;Zhi-Feng Zhang ,&nbsp;Meiliang Zhou ,&nbsp;Haihua Wang","doi":"10.1016/j.cropro.2025.107203","DOIUrl":"10.1016/j.cropro.2025.107203","url":null,"abstract":"<div><div>Golden buckwheat (<em>Fagopyrum dibotrys</em>) is a well-known edible herb with significant medicinal and economic values in China and other Asian countries. In May and June of 2022, an outbreak of leaf blight on <em>F. dibotrys</em> was observed in Weixi Lisu Autonomous County, Diqing Tibetan Autonomous Prefecture, Yunnan Province, China. The infected plants exhibit symptoms like leaf necrosis and wilt with frequent leaf curling or distortion. <em>Cladosporium</em>-like strains were consistently isolated from the diseased leaf tissues. Based on morphological characteristics and multi-gene (ITS, <em>tef1</em>, and <em>act</em>) phylogenetic analyses, the fungi were identified as <em>Cladosporium cladosporioides</em>. Pathogenicity tests showed that the selected <em>C. cladosporioides</em> strain could infect <em>F. dibotrys</em> leaves using non-wound inoculation. This represents the first report that <em>C. cladosporioides</em> causes leaf blight on <em>F. dibotrys</em> in China and the world.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107203"},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}