Laboratory and greenhouse endophytic colonization of tomato by three entomopathogenic fungal isolates for the management of Tetranychus urticae and field efficacy trials

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-09-03 DOI:10.1016/j.cropro.2025.107394

Waqas Wakil , Maria C. Boukouvala , Nickolas G. Kavallieratos , Aqsa Naeem , Syed Adnan Haider , Muhammad Usman Ghazanfar , Pasco B. Avery

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引用次数: 0

Abstract

Tetranychus urticae is a devastating arthropod in tomato crops, causing significant economic and yield losses globally. Several Metarhizium robertsii and Beauveria bassiana isolates have been extensively tested by direct application against T. urticae. Here, the endophytic colonization of M. robertsii (WG-04 and WG-05) and B. bassiana (WG-11) isolates on tomato plant parts was investigated by different inoculation methods. Their endophytic effect was investigated against T. urticae in the greenhouse and laboratory, and their acaricidal efficacy was evaluated in the field in 2020 and 2021, after their leaf-surface application. At 28 days after inoculation, WG-04 and WG-05 achieved high leaf colonization, reaching 95 % and 100 %, respectively, following foliar application, while WG-11 showed the lowest colonization rate (79 %). The three EPF as endophytes killed 75–94 % of nymphs and 69–85 % of adult females after 4 and 10 days, respectively, in laboratory trials. They also caused a considerable reduction in the establishment of T. urticae life stages on both surfaces of tomato leaves in greenhouse tests. In field trials, their direct application to mites on plants significantly decreased the mite population 8 days after treatment in both experimental years. Overall, WG-05 was the best EPF isolate that deserves further experimentation as an endophyte under field conditions against T. urticae.

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三种昆虫病原真菌在番茄室内和温室的内生定殖，用于荨麻疹叶螨的防治和田间药效试验

荨麻疹叶螨是危害番茄作物的节肢动物，在全球范围内造成了重大的经济和产量损失。一些罗伯特绿僵菌和球孢白僵菌分离株已经通过直接施用对荨麻疹菌进行了广泛的试验。采用不同的接种方法，研究了robertsim （WG-04和WG-05）和bassiana （WG-11）菌株在番茄植株上的内生定殖情况。在温室和室内研究了其对荨麻疹的内生效应，并于2020年和2021年对其叶面施用后的田间杀螨效果进行了评价。接种后28 d， WG-04和WG-05的叶片定殖率最高，分别达到95%和100%，而WG-11的定殖率最低，为79%。3种EPF作为内生菌，在4 d和10 d后分别杀死75 ~ 94%的若虫和69 ~ 85%的成虫。在温室试验中，它们还显著减少了在番茄叶片的两个表面上建立荨麻疹分枝杆菌的生命阶段。在田间试验中，在两个试验年份，将其直接施用于植物上的螨虫后8天，螨虫数量均显著减少。综上所述，WG-05是最好的EPF分离物，值得进一步在田间条件下作为内生菌进行抗荨麻疹试验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.