Crop Protection最新文献

{"title":"A rapid conductivity test offers a viable method to assess the quality of Bacillus thuringiensis products","authors":"Jorge Lima-Pérez ,&nbsp;César Gálvez ,&nbsp;Roberto Montesinos-Matías ,&nbsp;Nohemi Garcia-Ortiz ,&nbsp;Marco Antonio Mellin-Rosas ,&nbsp;Cynthia Coccet Castaneda-Casasola ,&nbsp;Jorge Antonio Sánchez-González","doi":"10.1016/j.cropro.2025.107109","DOIUrl":"10.1016/j.cropro.2025.107109","url":null,"abstract":"<div><div>Faster and less expensive quality tests are essential for measuring the quality of microbial pesticides formulations for users. In this work, the conductivity of the suspensions of six commercial <em>Bacillus thuringiensis</em> products was compared with the International Units of Potency (IU) in labels, the concentration of the pesticide protein, the presence of Cry-type, virulence in the larvae of <em>Spodoptera frugiperda</em> and <em>Helicoverpa zea</em>, spore and total viability, the presence of bacterial contaminants, pH, and humidity to establish relationships. The results showed that XenTari® had more viable spores (&gt;4.5 × 10¹⁰ spores • mL⁻¹), and Agree® had the most total viable cells. Dipel®, Agree®, and XenTari® had the most pesticide protein (&gt;40,000 μg mL⁻¹). The Cry-type 1 pesticide protein was present in Agree®, XenTari®, Turinsil®, and BtKrone®, and only Dipel® contained Cry-type 1 and type 2. Regarding virulence, a survival lower than 2.6% was obtained with Agree®, BtKrone®, DiPel®, and XenTari® products when <em>S</em>. <em>frugiperda</em> and <em>H</em>. <em>zea</em> larvae were used in the bioassay. Interestingly, while Bacimín® had the highest IU label claim, the pesticide protein and mortality of <em>S</em>. <em>frugiperda</em> larvae were not observed. However, viable cells and spores were detected at the lowest concentration (&lt;2 × 10⁷ spores • g⁻¹). These results were useful for classifying products into null, medium, and high quality categories and establishing relationships with conductivity. An important relationship was found between conductivity and protein concentration (R² &gt; 0.85) or survival (R² &gt; 0.81). Our findings showed that conductivity predicts the quality and performance of commercial <em>B. thuringiensis</em> products, suggesting that this method is a faster and more reliable quality test that can be easily used by farmers.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107109"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142986180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of monoterpenes, phenylpropenes, and sesquiterpenes for the management Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae)","authors":"Hanaa S. Hussein ,&nbsp;Sahar E. Eldesouky ,&nbsp;Samir A.M. Abdelgaleil","doi":"10.1016/j.cropro.2025.107110","DOIUrl":"10.1016/j.cropro.2025.107110","url":null,"abstract":"<div><div>The insecticidal, repellent, and oviposition-deterrent activities of 13 phytochemicals belonging to the monoterpene, phenylpropene, and sesquiterpene classes against <em>Bemisia tabaci</em> adults were evaluated on plants of tomato in laboratories and greenhouses. In the fumigant toxicity assay, <em>p</em>-cymene, α-terpinene, and α-pinene exhibited the highest fumigant toxicity with LC₅₀ values of 9.64, 11.74, and 14.35 μl/L air, respectively. In the contact toxicity assay, <em>trans</em>-cinnamaldehyde caused the highest insecticidal activity (LC₅₀ = 1.36 mg/L) followed by eugenol (1.42 mg/L). In contrast, farnesol, (<em>Z, E</em>)-nerolidol, α-pinene, α-terpinene, and <em>p</em>-cymene displayed a weak contact toxicity (LC₅₀ &gt; 10 mg/L). The highest contact toxicity in the greenhouse experiment was also observed with the <em>trans</em>-cinnamaldehyde (83.45%), followed by eugenol (80.62%), compared with thiamethoxam (87.75%) at 10 mg/L and 72h post-treatment. The maximum repellency was observed with <em>trans</em>-cinnamaldehyde (80.45%), eugenol (79.64%), (−)-citronellal (78.93%), (1R,2S,5R)-menthol (78.17%), and (−)-carvone (73.52%) at concentration of 10 mg/L after 24h compared to thiamethoxam (89.26%). Moreover, the greatest oviposition deterrence percentage was observed after 24 h for <em>trans</em>-cinnamaldehyde (52.30%), and eugenol (48.56%) at 10 mg/L compared to thiamethoxam (46.68%). The results indicate that these compounds may be employed as bioinsecticides for the control of <em>B. tabaci</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107110"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a rapid detection system for Phytophthora syringae based on RPA/CRISPR-cas12a","authors":"YuFang Guo ,&nbsp;Jiajin Tan ,&nbsp;Binbin Jiao ,&nbsp;Fengqi Wang ,&nbsp;Haiwen Wang ,&nbsp;Chun Yang ,&nbsp;Tingting Dai","doi":"10.1016/j.cropro.2024.107106","DOIUrl":"10.1016/j.cropro.2024.107106","url":null,"abstract":"<div><div><em>Phytophthora syringae</em>, classified into algae Eumycophyta, Oomycota, Pythiales, Pythiaceae, which is a quarantine pathogen that causes brown rot on fruits, stem rot and root rot in urban landscaping. Although the existence of <em>P. syringae</em> has not been proven in China. Since the first interception of <em>P. syringae</em> on navel oranges imported from the United States at the port of Tianjin in 2011, quarantine authorities in various locations have repeatedly found <em>Phytophthora</em> in imported fruits. However, traditional detection methods such as morphological identification and polymerase chain reaction are time-consuming and require high levels of expertise and technical requirements for detection personnel. In this study, recombinase polymerase amplification (RPA) in combination with the CRISPR/Cas12a system to identify <em>P. syringae</em> was developed. The assay was found to be highly specific to <em>P. syringae</em> without detection of other tested species including <em>P. hibernalis</em>, <em>P. palmivora</em>, <em>P. nicotianae</em>, and <em>P. citrophthora</em>, four other important pathogens on citrus. And this assay is sensitive and detects 100 pg μL⁻¹ <em>P. syringae</em> genomic DNA at 37 °C in 45 min. The test results were visible under ultraviolet (UV) light and provided a fluorophore readout. This innovative test can detect <em>P. syringae</em> in naturally inoculated citrus fruit seeds. The RPA-CRISPR/Cas12a detection assay developed in this study is characterized by its sensitivity, efficiency and ease of use. Early detection and control of <em>P. syringae</em> are crucial for the protection of urban green cover species.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107106"},"PeriodicalIF":2.5,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism and synergy evaluation of a mixture of croton essential oil and Spirodiclofen on Phenacoccus solenopsis (Hemiptera: Pseudococcidae)","authors":"Guangmin Ou ,&nbsp;Shiqi Zhu ,&nbsp;Qiurong Huang ,&nbsp;Yanhua Wei ,&nbsp;Zhixiang Zhang ,&nbsp;Dongmei Cheng","doi":"10.1016/j.cropro.2024.107104","DOIUrl":"10.1016/j.cropro.2024.107104","url":null,"abstract":"<div><div>As a major invasive injurious insect, <em>Phenacoccus solenopsis</em> Tinsley poses a significant threat to cotton production. The presence of a wax coating on <em>P. solenopsis</em> impedes the efficacy of insecticides and contributes to the development of chemical pesticide resistance. Therefore, the synergistic effect and mechanism of croton essential oil (Co) and its components were investigated in combination with spirodiclofen, which has shown a superior synergistic effect on wax, to effectively manage this pest and mitigate the development of resistance. The compounds Co, α-pinene, fenchyl alcohol, and camphene exhibited favorable solubility in the wax secretions of mature female <em>P. solenopsis</em>, leading to a notable synergistic impact on spirodiclofen. The measured contact angles between the essential oil mixtures and beeswax were 22.27°, 26.94°, 29.72°, and 19.37°. The penetration levels of spirodiclofen were 1.65, 1.47, 1.26, and 1.45 μg, resulting in synergistic ratios of 3.51, 3.51, 3.11, and 3.37, and the inhibitory effect of increased essential oils on GST activity was 36.53%, 30.48%, 54.22% and 59.38%, respectively. The results showed that when used in combination with spirodiclofen, these compounds alone or in combination reduced the GST activity of the females, with the best synergistic effect of fenchyl alcohol with spirodiclofen, and Co and its constituents showed good synergistic effect. Thus, this method shows good potential for the development of additives and the results proved a new material source for green pest control.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107104"},"PeriodicalIF":2.5,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility monitoring and metabolic resistance study of Echinochloa crus-galli to three common herbicides in rice regions of the Mid-Lower Yangtze, China","authors":"Tangqi Feng ,&nbsp;Wenjing Sun ,&nbsp;Jian Wang ,&nbsp;Tianhong Lei ,&nbsp;Lei Wang ,&nbsp;Yuanli Xie ,&nbsp;Huazhong Zhou ,&nbsp;Fuxing Zhu ,&nbsp;Hongju Ma","doi":"10.1016/j.cropro.2024.107108","DOIUrl":"10.1016/j.cropro.2024.107108","url":null,"abstract":"<div><div><em>Echinochloa crus-galli,</em> a dominant weed in rice fields, has developed increasing herbicide resistance, complicating its control. This study evaluated the susceptibility of 30 <em>E. crus-galli</em> field populations to three commonly used herbicides: metamifop (ACCase inhibitor), penoxsulam (ALS inhibitor), and quinclorac (auxin herbicide). Resistance ratio (RI) ranged from 1.1- to 7.9-fold for metamifop, 1.1- to 27.9-fold for penoxsulam, and 1.3- to 55.9-fold for quinclorac. A gradual decline in herbicide efficacy was observed, with an increasing number of multi-resistant populations over time. Multi-resistant populations (CB18, CB19, CB20, JL20, NX20) exhibited significantly higher P450 and GST enzyme activities compared to susceptible populations (S0). Furthermore, genes involved in metabolic resistance, including <em>CYP81A12</em>, <em>CYP81A14</em>, <em>CYP81A21</em>, <em>CYP81A68</em>, and <em>GSTU23</em>, were significantly upregulated in multi-resistant populations. This study provides insights into the resistance dynamics of <em>E. crus-galli</em> in the Mid-Lower Yangtze rice region, contributing valuable data for improving herbicide management strategies and resistance mitigation.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"191 ","pages":"Article 107108"},"PeriodicalIF":2.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143325986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field evaluation of new fungicides in controlling downy mildew of broccoli - A mixed-effects model analysis","authors":"Ranbir Singh ,&nbsp;Diksha Loona ,&nbsp;Amoghavarsha Chittaragi ,&nbsp;Balanagouda Patil","doi":"10.1016/j.cropro.2024.107107","DOIUrl":"10.1016/j.cropro.2024.107107","url":null,"abstract":"<div><div>Broccoli (<em>Brassica oleracea</em> var. <em>italica</em>) is a nutritionally rich vegetable, but its production is hampered by downy mildew, caused by <em>Hyaloperonospora parasitica</em>. This study aimed to evaluate the efficacy of various fungicides in managing downy mildew in broccoli under field conditions during the 2021-22 and 2022-23 growing seasons. Nine treatments, including fungicidal combinations and solo products, were tested in a randomized complete block design. Disease index and yield were assessed at 15 day intervals post-application. The results showed that Fluopicolide + Fosetyl Al significantly reduced disease, with a pooled disease index (DI) of 12.77 and increased yield to 244.00 g/head, compared to untreated control with 34.43 DI and 122.00 g/head. Similarly, Famoxadone + Cymoxanil and Fenamidone + Mancozeb were highly effective, with DIs of 16.82 and 17.93 respectively, and increased yields. A regression analysis indicated a strong negative correlation between disease and yield (R² = 0.887), with each 1% increase in disease resulting in a 5.56 g/head yield reduction. The mixed-effects model analysis revealed significant treatment effects while accounting for year variability. The study concludes that integrating fungicides with different modes of action, such as Fluopicolide + Fosetyl Al, can effectively manage downy mildew while optimizing yield. This research emphasizes the need for strategic fungicide application to prevent resistance development and enhance sustainable broccoli production.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107107"},"PeriodicalIF":2.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance of several Indonesian cereal grain cultivars to the maize weevil, Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae)","authors":"M. Bayu Mario ,&nbsp;Muhammad Taufik ,&nbsp;Melina Melina ,&nbsp;Ravindra Chandra Joshi ,&nbsp;Idul Anshar ,&nbsp;Paridah Paridah ,&nbsp;Ahmad Komaini ,&nbsp;Lekhnath Kafle ,&nbsp;Samir A.M. Abdelgaleil ,&nbsp;Eirene Brugman ,&nbsp;Ito Fernando","doi":"10.1016/j.cropro.2024.107105","DOIUrl":"10.1016/j.cropro.2024.107105","url":null,"abstract":"<div><div>The maize weevil, <em>Sitophilus zeamais</em> (Motschulsky) (Coleoptera: Curculionidae), is a primary pest and internal feeder of cereal grains, responsible for substantial economic losses worldwide, including in Indonesia. This study assessed the host preference and biological performance of the maize weevil on several Indonesian cereal grains, including milled rice (IR64), unhulled rice (Mekongga), maize seed (Jakarin), and sorghum seed (Soper 7). In a free-choice preference test, sorghum seed was the most preferred host for adult weevils to feed on and lay eggs, followed by milled rice, maize seed, and unhulled rice. The susceptibility index from the antibiosis test indicated that unhulled rice and maize seed were resistant, whereas milled rice and sorghum seed were classified as susceptible. The preadult period (38.11–43.43 days) and median development period (30.13–41.63 days) of <em>S. zeamais</em> were positively correlated with tannin, phenolic, and carbohydrate contents in the grains. Conversely, the preadult period, median development period, and F₁ progeny were negatively correlated with grain hardness and lipid content, while grain moisture content showed a negative correlation with the preadult period and median development period. Additionally, the seed coat on unhulled rice appeared to act as a barrier that inhibited feeding and oviposition by <em>S. zeamais</em>. Therefore, the host preference and biology of <em>S. zeamais</em> were associated with the physicochemical properties of the evaluated cereal grains. Consequently, evaluating the susceptibility of cereal grains to <em>S. zeamais</em> is fundamental for developing effective integrated pest management strategies to mitigate the economic impact of this pest.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107105"},"PeriodicalIF":2.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence, molecular identification and phylogenetic analyses of viruses associated with garlic viral complex in Nepal","authors":"Subhas Neupane ,&nbsp;Hae-Ryun Kwak ,&nbsp;Nabin Sharma Poudel ,&nbsp;Nisha Rokaya ,&nbsp;Ritesh Kumar Yadav ,&nbsp;Sundar Man Shrestha ,&nbsp;Hong-Soo Choi ,&nbsp;Hira Kaji Manandhar","doi":"10.1016/j.cropro.2024.107103","DOIUrl":"10.1016/j.cropro.2024.107103","url":null,"abstract":"<div><div>Garlic is a major spice crop in Nepal. A number of pathogens, including viruses, are known to cause economically important diseases in garlic. Garlic leaves showing leaf curl and mosaic symptoms and thirteen garlic accessions were collected from 12 districts. The accessions were planted under both the field and vector-free conditions. The leaf samples, collected directly from the fields and representative leaf samples from both field and vector-free growing conditions, were subjected to reverse transcription polymerase chain reaction (RT-PCR). Species of <em>Potyvirus</em> [(onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV)], <em>Carlavirus</em> [(shallot latent virus (SLV) and garlic common latent virus (GarCLV)], and <em>Allexivirus</em> [garlic virus A, D, H, and X (GarV-A, -D, -H, and –X)] were detected in mixed infections. Sequence and phylogenetic analyses showed that LYSV, OYDV, and GarCLV were closest to Indian isolates (including one LYSV isolate close to an Iranian isolate), SLV was close to Chinese isolates, and allexiviruses were close to Indian, Korean, and Chinese isolates. To our knowledge, this is the first report of SLV, GarV-A, GarV-D, GarV-H, and GarV-X in Nepal. The findings warrant plant quarantine measures and concerted research for their effective management.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107103"},"PeriodicalIF":2.5,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of Helicoverpa armigera via plant-mediated RNA interference, progresses and challenges","authors":"Mingming Jiang ,&nbsp;Yao Zhang ,&nbsp;Chuming Chen,&nbsp;Ludan He,&nbsp;Shengchun Li","doi":"10.1016/j.cropro.2024.107101","DOIUrl":"10.1016/j.cropro.2024.107101","url":null,"abstract":"<div><div>Cotton bollworm <em>Helicoverpa armigera</em> (Hübner) (Lepidoptera Noctuidae) is a major agricultural pest that causes significant yield losses of crop plants each year. In the past few decades, the potent approaches of <em>H</em>. <em>armigera</em> control, including chemical insecticides and <em>Bacillus thuringiensis</em> (<em>Bt</em>) toxins, have been constrained due to health hazards, environmental contamination, and development of resistance, after their extensive application. Thus, there is need to find alternative strategies for <em>H</em>. <em>armigera</em> management. Expression of insect-targeted RNA interference (RNAi) constructs in host plants has emerged as one of such a novel and environment friendly strategies. However, various factors, such as highly active nucleases and high pH in the midgut, as well as failure of small interfering RNA to escape the endosome, lead to relatively low RNAi efficiency in lepidopteran insects. Although most plant-mediated RNAi experiments compromised insect development and growth of <em>H</em>. <em>armigera</em>, full protection of transgenic plants against <em>H</em>. <em>armigera</em> was not achieved and no commercially available product has yet been developed for <em>H</em>. <em>armigera</em> control. A clear understanding of the current status of RNAi utilization in <em>H</em>. <em>armigera</em> control is critical for improving its efficiency. In this review, we summarize the recent advances in plant-mediated RNAi for <em>H</em>. <em>armigera</em> control, including target gene selection, double stranded RNA or artificial microRNA expression from nuclear or plastid genome, and protection of RNAi molecules from degradation by nucleases. Additionally, we highlight factors influencing the efficacy of plant-mediated RNAi, and discuss the future research of this plant genetic engineering strategy for achieving effective management of <em>H. armigera</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107101"},"PeriodicalIF":2.5,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142986182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticide resistance status and vector potential of Bemisia tabaci populations on vegetable crops in Türkiye","authors":"Arda İnak ,&nbsp;Berke Demirci ,&nbsp;Esengül Erdem ,&nbsp;Filiz Randa-Zelyüt ,&nbsp;Ali Karanfil ,&nbsp;Ahmednur Yusuf Idan ,&nbsp;Umut Toprak ,&nbsp;Emre İnak ,&nbsp;Thomas Van Leeuwen","doi":"10.1016/j.cropro.2024.107097","DOIUrl":"10.1016/j.cropro.2024.107097","url":null,"abstract":"<div><div>The sweet potato whitefly <em>Bemisia tabaci</em> (Gennadius, 1889) (Hemiptera: Aleyrodidae), is an economically important polyphagous pest species with a global distribution. This pest not only causes direct damage by sucking plant phloem sap but also transmits viruses and excretes honeydew, which can lead to the formation of black sooty mould, thereby intensifying its significance in agricultural regions. Although chemical insecticides have been extensively used for whitefly control, the development of resistance leading to control failures has been frequently documented. Here, we have initially assessed the efficacy of four commonly used insecticides (acetamiprid, sulfoxaflor, spirotetramat, cyantraniliprole) against whitefly populations through greenhouse trials across eight distinct locations. Additionally, we conducted a comprehensive molecular screening of 35 field populations to identify resistance mutations at the insecticide target sites and to detect plant pathogenic viruses. The results revealed that sulfoxaflor and cyantraniliprole exhibited the highest efficacy against nymphal stages of whiteflies, whereas acetamiprid was determined to be the most effective insecticide against adult stages. Several well-known target-site mutations in acetylcholinesterase (F331W), voltage-gated sodium channel (VGSC; M918L, L925I, T929V), and acetyl-CoA carboxylase (A2083V) were found to be widespread in <em>Turkish B. tabaci</em> populations. Additionally, two mutations, I936V and I936F, previously associated with pyrethroid resistance, were identified for the first time in the VGSC of <em>B. tabaci</em>. Conversely, no amino acid substitutions were detected in the amplified fragments of the ryanodine and nicotinic acetylcholine receptors. Furthermore, tomato chlorosis virus (ToCV) was detected in five field populations from Antalya. The widespread distribution of whitefly populations with multiple resistance mutations underscores the necessity of implementing integrated pest management programs in Turkish vegetable production areas.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"190 ","pages":"Article 107097"},"PeriodicalIF":2.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}