Identification and characterization of Colletotrichum boninense causing anthracnose on Epimedium sagittatum in China

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-10-11 DOI:10.1016/j.cropro.2025.107444

Guiying Liu , Lu Wang , Kaihua Peng , Xincun Wang , Huixin Li , Mingrui Liu , Xiuhai Gan , Xiaomao Wu , Yong Wang , Zaifu Yang

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引用次数: 0

Abstract

Epimedium sagittatum, an important traditional Chinese medicinal herb valued for its tonic and anti-rheumatic properties, was found to be affected by leaf anthracnose in Jiangkou County, Tongren City, Guizhou Province, in 2024 and 2025. Symptoms were observed on both nursery seedlings and field-grown plants. Fungal isolates resembling Colletotrichum species were consistently obtained from symptomatic leaf tissues. Morphological examination combined with multilocus sequence analysis of ITS, act, tub2, cal, and gapdh regions identified the pathogen as Colletotrichum boninense. Phylogenetic inference and morphological traits supported this classification. Pathogenicity was verified through inoculation of healthy E. sagittatum leaves with isolate GE001, which reproduced symptoms consistent with field observations. The fungus was successfully re-isolated from the diseased leaves and confirmed using morphological and molecular approaches, thereby fulfilling Koch's postulates. To our knowledge, this study represents the first report of C. boninense causing anthracnose on E. sagittatum. These findings reveal a novel fungal threat to this economically significant medicinal plant and emphasize the necessity for further research into disease management strategies.

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矢状淫羊藿炭疽病病原菌的鉴定与鉴定

贵州省铜仁市江口县于2024年和2025年发现淫羊藿（Epimedium sagittatum）叶炭疽病，是一种具有滋补和抗风湿作用的重要中药材。苗圃幼苗和田间种植的植物均有症状。从有症状的叶片组织中分离出类似炭疽菌的真菌。形态学检查结合ITS、act、tub2、cal和gapdh区域的多位点序列分析，鉴定病原菌为炭疽杆菌。系统发育推断和形态特征支持这种分类。用分离物GE001接种健康的矢状叶，证实其致病性，其症状与现场观察一致。真菌成功地从患病叶片中重新分离出来，并通过形态学和分子方法证实，从而实现了科赫的假设。据我们所知，这项研究是第一次报道C. boninense引起弓形虫炭疽病。这些发现揭示了一种新的真菌对这种具有重要经济意义的药用植物的威胁，并强调了进一步研究疾病管理策略的必要性。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.