Critical Reviews in Microbiology最新文献_第2页

How force drives pathogenicity: mechanoregulation of Streptococcus mutans adhesion to collagen. 力如何驱动致病性：变形链球菌黏附于胶原蛋白的机械调节。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-05-01 Epub Date: 2025-11-12 DOI: 10.1080/1040841X.2025.2584074

Carla Inostroza, Pablo Berríos, Ivana Orellana, Jaime Andrés Rivas-Pardo, Sebastian Aguayo

{"title":"How force drives pathogenicity: mechanoregulation of Streptococcus mutans adhesion to collagen.","authors":"Carla Inostroza, Pablo Berríos, Ivana Orellana, Jaime Andrés Rivas-Pardo, Sebastian Aguayo","doi":"10.1080/1040841X.2025.2584074","DOIUrl":"10.1080/1040841X.2025.2584074","url":null,"abstract":"Streptococcus mutans is a caries-associated bacterium with the ability to adhere to the surface of oral tissues and promote biofilm formation. For this purpose, S. mutans expresses a range of specialized surface adhesins, among which collagen-binding proteins (CBPs) have demonstrated an important function regarding attachment to dentin, bacterial coaggregation, and extracellular matrix invasion. Understanding the mechanobiological behavior of CBPs, particularly their interaction with collagens during the process of bacterial adhesion, is crucial for developing novel strategies to prevent biofilm formation in oral and remote tissues. Therefore, this review summarizes recent evidence regarding the main mechanical properties of the relevant S. mutans CBPs SpaP, WapA, Cnm, and Cbm, and how their mechanobiological and adhesive characteristics play an important role in their virulence toward the host. Particularly, we will focus on how state-of-the-art interdisciplinary approaches such as atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) and single-cell force spectroscopy (SCFS) have been employed to characterize S. mutans and CBP attachment to collagen substrates and mechanical behavior in real-time and under physiological conditions. Altogether, the potential use of AFM SMFS and SCFS to explore novel anti-biofilm molecules against S. mutans remains an exciting possibility for the development of caries-preventive treatments in the future.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"483-499"},"PeriodicalIF":5.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145494930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-inspired anti-virulence strategies against membrane-targeting bacterial toxins: modification and mimicry. 宿主启发抗膜靶向细菌毒素的毒力策略：修饰和模仿。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-05-01 Epub Date: 2025-12-25 DOI: 10.1080/1040841X.2025.2605546

O'Rorke K Smith, Lyn M Wise, Jeremy Simcock, Dhammika Leshan Wannigama, Eduard B Babiychuk, Daniel Pletzer

{"title":"Host-inspired anti-virulence strategies against membrane-targeting bacterial toxins: modification and mimicry.","authors":"O'Rorke K Smith, Lyn M Wise, Jeremy Simcock, Dhammika Leshan Wannigama, Eduard B Babiychuk, Daniel Pletzer","doi":"10.1080/1040841X.2025.2605546","DOIUrl":"10.1080/1040841X.2025.2605546","url":null,"abstract":"Pore-forming toxins (PFTs) are essential virulence factors produced by many bacterial pathogens, enabling tissue invasion, nutrient acquisition, and immune evasion. Neutralizing these toxins offers a promising therapeutic avenue to mitigate infection symptoms and slow disease progression. Recent research highlights the potential of host-inspired strategies targeting toxin-membrane interactions. Statins and oxysterols disrupt intracellular cholesterol synthesis and trafficking to reduce its abundance in cell membranes, mimicking natural cellular defenses against PFTs. Aminosterols alter membrane properties to hinder toxin binding and pore formation. Nanoparticle-based decoys, such as artificial liposomes composed of the lipids cholesterol and sphingomyelin or recycled cell membranes, act as toxin traps, sequestering PFTs to protect host tissues. These nanoparticles demonstrate broad-spectrum efficacy across many bacterial species and offer additional functions, such as scavenging inflammatory cytokines. This review evaluates the clinical potential of these emerging treatment strategies and discusses the advantages of leveraging host factors to mitigate bacterial virulence rather than directly targeting toxins. Such host-inspired approaches represent a novel and complementary addition to the arsenal against antibiotic-resistant bacterial pathogens.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"611-629"},"PeriodicalIF":5.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in omics-driven research of photosynthetic microorganisms in green biotechnology. 绿色生物技术中光合微生物组学驱动研究进展。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-04-20 DOI: 10.1080/1040841X.2026.2655676

Boris Sorokin, Evgeniy Gusev, Zorigto Namsaraev, Anton Buzdin, Alina Osmakova, Denis Kuzmin

{"title":"Recent advances in omics-driven research of photosynthetic microorganisms in green biotechnology.","authors":"Boris Sorokin, Evgeniy Gusev, Zorigto Namsaraev, Anton Buzdin, Alina Osmakova, Denis Kuzmin","doi":"10.1080/1040841X.2026.2655676","DOIUrl":"https://doi.org/10.1080/1040841X.2026.2655676","url":null,"abstract":"Microalgae and cyanobacteria have attracted significant interest from green biotechnology and the bioeconomy due to their numerous beneficial qualities: extremely high growth rate compared to vascular plants; independence from arable land or clean water; ability to produce a variety of industrially important bioproducts, such as biofuels, food and feed additives, and substances for the cosmetics and pharmaceutical industries. They also offer the possibility of combining bioproduction with industrial carbon dioxide capture and wastewater utilization. One of the main limitations to the widespread use microalgae is the economic feasibility of their cultivation. Consequently, researchers in the field are primarily concerned with the identification of novel high-productive strains and the augmentation of biomass and/or target metabolite yield. Omics technologies are the key to unlocking the biotechnological potential of photosynthetic microorganisms, enabling significant acceleration in the identification of novel, efficient strains and providing valuable insights into the microbial metabolism. The present review focuses on the latest advances in the field of omics-driven research of photosynthetic microorganisms and provides a comprehensive outlook of practical instruments, such as open-access omics databases and stoichiometric metabolic models, that can improve the efficiency of biotechnological research pipelines to achieve breakthroughs in the industrial application of microalgae and cyanobacteria.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"1-34"},"PeriodicalIF":5.1,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial kinase in ESKAPE pathogen: regulators of virulence and novel therapeutic targets. ESKAPE病原体中的细菌激酶：毒力调节剂和新的治疗靶点。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-04-18 DOI: 10.1080/1040841X.2026.2655678

Saroj Sharma, Vishvanath Tiwari

{"title":"Bacterial kinase in ESKAPE pathogen: regulators of virulence and novel therapeutic targets.","authors":"Saroj Sharma, Vishvanath Tiwari","doi":"10.1080/1040841X.2026.2655678","DOIUrl":"https://doi.org/10.1080/1040841X.2026.2655678","url":null,"abstract":"Bacterial protein kinases regulate fundamental processes like cell division and transcription, hence controlling various metabolic pathways. They share a common evolutionary origin with eukarya and archaea. Kinases promote the virulence of pathogenic bacteria and are also involved in the host-pathogen interactions leading to the manipulation of host defence systems for the establishment of infections. Bacterial protein kinases regulate various metabolic processes, including pathogenesis, virulence, upregulation of efflux pumps to discard antibiotics, synthesis of capsular polysaccharides, and pathogenesis. Bacterial kinases offer a new aspect of monitoring and managing the infections caused by bacterial pathogens. As kinases are essential proteins of the bacterial system, there are lesser chances of mutation in them. Hence, therapeutics targeting the pathogenic bacterial kinases would have significant value. This review deals with the functions and structural aspects of bacterial protein kinases, and the different types of kinase inhibitors that have been experimentally validated to control infections. They represent promising therapeutic target, as they will minimize the risk of gaining new types of resistance and offer a high probability of success, given their role as master regulators of the fundamental processes of the bacterial system.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"1-14"},"PeriodicalIF":5.1,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147716351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of Klebsiella pneumoniae pathogenicity: from virulence factors to emerging therapeutic strategies. 肺炎克雷伯菌致病性的分子机制：从毒力因素到新兴的治疗策略。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-03-30 DOI: 10.1080/1040841X.2026.2648208

Hongjie Hou, Xiaohui Zhou

{"title":"Molecular mechanisms of Klebsiella pneumoniae pathogenicity: from virulence factors to emerging therapeutic strategies.","authors":"Hongjie Hou, Xiaohui Zhou","doi":"10.1080/1040841X.2026.2648208","DOIUrl":"https://doi.org/10.1080/1040841X.2026.2648208","url":null,"abstract":"Klebsiella pneumoniae has emerged as a major global health threat, contributing significantly to neonatal sepsis, childhood mortality, and antibiotic-resistant hospital-acquired infections. The World Health Organization (WHO) recently reported the widespread detection of hypervirulent K. pneumoniae (hvKP) strains carrying carbapenem resistance genes across all WHO regions, prompting a formal warning from the Global Antimicrobial Resistance Surveillance System. Despite growing concerns, the mechanisms driving the enhanced pathogenicity and adaptability of these strains remain incompletely understood. Recent research has uncovered novel virulence factors and elucidated key adaptations enabling K. pneumoniae to thrive in diverse host tissues. This review synthesizes current knowledge on K. pneumoniae pathogenicity, focusing on virulence factors, immune evasion strategies, and phenotypic plasticity. Additionally, we explore emerging anti-virulence strategies as potential therapeutic interventions. By consolidating these insights, this review aims to guide future research and inform the development of new approaches to address this escalating public health challenge.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"1-32"},"PeriodicalIF":5.1,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Friends and foes: impact of bacteria on genome stability. 朋友与敌人：细菌对基因组稳定性的影响。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-03-18 DOI: 10.1080/1040841X.2026.2641726

Goran Gajski, Olga Szewczyk-Roszczenko, Piotr Roszczenko, Yegor Vassetzky, Nikolajs Sjakste

{"title":"Friends and foes: impact of bacteria on genome stability.","authors":"Goran Gajski, Olga Szewczyk-Roszczenko, Piotr Roszczenko, Yegor Vassetzky, Nikolajs Sjakste","doi":"10.1080/1040841X.2026.2641726","DOIUrl":"https://doi.org/10.1080/1040841X.2026.2641726","url":null,"abstract":"Bacteria, present in normal conditions in the microbiome or during infections, exert profound effects on genome stability, with both genotoxic and genoprotective consequences. Certain pathogenic bacteria, such as Escherichia coli (colibactin-producing strains), Helicobacter pylori, Fusobacterium nucleatum, and Campylobacter jejuni, induce DNA damage and are implicated in cancer development through direct toxin production, chronic inflammation, immune modulation, and disruption of host cell signaling. Genotoxins such as colibactin, the cytolethal distending toxin, and the typhoid toxin induce DNA double-strand breaks, chromosomal instability, and impair DNA repair pathways, contributing to carcinogenesis. These effects occur upon gastrointestinal, urogenital, systemic (sepsis), and neurological (meningitis) infections, in both humans and animals. Conversely, commensal and probiotic bacteria, notably Lactobacillus and Bifidobacterium species, play a protective role by reducing oxidative DNA damage, modulating immune responses, and enhancing DNA repair. Their beneficial actions are partly mediated by metabolites such as short-chain fatty acids (e.g. butyrate), which influence gene regulation, apoptosis, and mucosal health. Probiotic bacteria can mitigate the genotoxic effects of dietary and bacterial toxins, offering a potential preventive strategy against genome instability and cancer. This review highlights the dualistic nature of bacterial influence on host genome integrity and underscores the importance of maintaining microbial balance.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":5.1,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the ambiguity behind Enterococcus as a probiotic ally or a pathogenic adversary. 理解肠球菌作为益生菌盟友或致病对手背后的模糊性。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-03-01 Epub Date: 2025-09-18 DOI: 10.1080/1040841X.2025.2562926

Arxel G Elnar, Geun-Bae Kim

{"title":"Understanding the ambiguity behind Enterococcus as a probiotic ally or a pathogenic adversary.","authors":"Arxel G Elnar, Geun-Bae Kim","doi":"10.1080/1040841X.2025.2562926","DOIUrl":"10.1080/1040841X.2025.2562926","url":null,"abstract":"The genus Enterococcus has been extensively investigated, emphasizing either its potential as a probiotic in fermented foods or its opportunistic pathogenic nature, particularly in hospital settings. This review focuses on the defining characteristics of enterococci species to better understand their dual nature, with the goal of establishing a universal method to safely and effectively characterize probiotic enterococci. Despite harboring genes for potentially harmful enzymes and metabolites, enterococci have traditionally been used to improve the flavor profiles of artisanal dairy products. Additionally, certain strains produce antimicrobial compounds, particularly bacteriocins, which help control the microbial composition of food products. These bacteriocins have been extensively explored as alternatives to antibiotics, driven by the rapid increase in antimicrobial resistance across several bacterial species. However, enterococcal isolates of nosocomial origin are harmful. Recent studies have clarified the divergent lineages that resulted in the emergence of pathogenic and nosocomial strains, thus improving the selection process for determining whether an isolate can be utilized as a probiotic. The insights gathered in this review have important implications for developing regulations on and optimizing the use of enterococci.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"350-376"},"PeriodicalIF":5.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond tellurite: the multifunctional roles of genes annotated as tellurium resistance determinants in bacteria. 超越碲：在细菌中作为碲抗性决定因素的基因的多功能作用。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-03-01 Epub Date: 2025-09-10 DOI: 10.1080/1040841X.2025.2555936

Linda Darwiche, Jennifer L Goff

{"title":"Beyond tellurite: the multifunctional roles of genes annotated as tellurium resistance determinants in bacteria.","authors":"Linda Darwiche, Jennifer L Goff","doi":"10.1080/1040841X.2025.2555936","DOIUrl":"10.1080/1040841X.2025.2555936","url":null,"abstract":"The metalloid tellurium (Te) is toxic to bacteria; however, the element is also extremely rare. Thus, most bacteria will never encounter Te in their environment. Nonetheless significant research has been performed on bacterial Te resistance because of the medical applications of the element. The so-called \"tellurium resistance (TeR) genes\" were first described on plasmids isolated from clinically relevant Enterobacteriaceae. With time, it has become apparent that, given the rarity of Te on the planet, these genes may have functions beyond tellurium resistance. Nonetheless, the description of these genes as \"tellurium resistance genes\" has persisted. In this review, we first examine the history and discovery of the TeR genes. We then performed an analysis of 184,000 high-quality, prokaryotic (meta)genomes, which revealed that terZABCDF, telA, and tehAB are relatively common in genome annotations and that they are frequently described as \"tellurium resistance genes\". We synthesized the literature to describe the functions of these ubiquitous genes beyond tellurium resistance. These genes have functions in diverse cellular processes including phage resistance, antibiotic resistance, virulence, oxidative stress resistance, cell cycle regulation, metal resistance, and metalation of exoenzymes. Considering this analysis, we propose that it is time to appreciate the multifunctional nature of the \"tellurium resistance genes\".","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"262-282"},"PeriodicalIF":5.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current and emerging therapies for fungal biofilms and systemic infections. 目前和新兴的治疗真菌生物膜和全身性感染。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-03-01 Epub Date: 2025-10-10 DOI: 10.1080/1040841X.2025.2570185

Yehia Elgammal, Rafael I Garcia Martinez, Adrian Requejo, Luis R Martinez

引用次数: 0

Under the lens: using Raman spectroscopy as a unique system in biofilm analyses. 镜头下：利用拉曼光谱作为生物膜分析的独特系统。

IF 5.1 2区生物学

Critical Reviews in Microbiology Pub Date : 2026-03-01 Epub Date: 2025-09-06 DOI: 10.1080/1040841X.2025.2555937

Zainab Bilal, William Tipping, Jason L Brown, Karen Faulds

{"title":"Under the lens: using Raman spectroscopy as a unique system in biofilm analyses.","authors":"Zainab Bilal, William Tipping, Jason L Brown, Karen Faulds","doi":"10.1080/1040841X.2025.2555937","DOIUrl":"10.1080/1040841X.2025.2555937","url":null,"abstract":"Biofilms are microbial communities that adhere to surfaces and each other, encapsulated in a protective extracellular matrix. These structures enhance resistance to antimicrobials, contributing to 65-80% of human infections. The transition from free-living cells to structured biofilms involves a myriad of molecular and structural adaptations. Raman spectroscopy is an analytical technique that has recently been adapted for biofilm analysis. The ability to operate without interference from water makes Raman spectroscopy a valuable tool for in situ characterization of biofilms, including direct analysis from clinical samples. The technique also offers the advantage of imaging speed and the capacity to generate extensive chemical and molecular data from samples, whilst also being non-destructive. However, Raman spectroscopy is often limited by its low sensitivity, particularly when applied to microbial analysis. This limitation has been addressed with the advent of surface-enhanced Raman spectroscopy and stimulated Raman scattering microscopy. When used in combination with traditional methods, these Raman technologies can be incredibly useful for understanding the mechanisms underlying biofilm development, antimicrobial susceptibility testing, and detection and discrimination of microorganisms. In this critical review, the application of Raman spectroscopy and its derivatives as a tool for biofilm characterization is discussed along with its associated advantages and challenges.","PeriodicalId":10736,"journal":{"name":"Critical Reviews in Microbiology","volume":" ","pages":"283-301"},"PeriodicalIF":5.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0