Clinical Oral Investigations最新文献

{"title":"Brushing motion caused no microcracks: a micro-computed tomography study.","authors":"Deniz Yanık, Şelale Özel, Fügen Dağlı Taşman Cömert","doi":"10.1007/s00784-025-06253-0","DOIUrl":"10.1007/s00784-025-06253-0","url":null,"abstract":"<p><strong>Objective: </strong>We evaluated the effect of brushing motion on microcrack formation in round distal canals after using multi-file rotary(MFR), single-file rotary(SFR), and single-file reciprocation(SFRc) systems via micro-computed tomography(micro-CT).</p><p><strong>Materials and methods: </strong>Thirty-six mandibular molars were used. Samples were allocated according to files and preparation patterns (n = 12); pecking (P) and brushing (B): Group-MFR-P, Group-MFR-B, Group-SFRc-P, Group-SFRc-B, Group-SFR-P, Group-SFR-B. MFR was ProTaper Next, SFR was TruNatomy, and SFRc was WaveOne Gold. Mesial and distal were prepared using pecking motion, and additional brushing motion. Brushing motions were performed after the pecking motions with 6 strokes. Pre-and-post-instrumentation scans were obtained. Wilcoxon, Krukal-Wallis, and Mann-Whitney-U were performed.</p><p><strong>Results: </strong>No differences were between pre-and-post-instrumentation scans (p > 0.05). Post-instrumentation microcracks were not different in Group MFR-P and Group MFR-B, Group SFRc-P and Group SFRc-B, Group SFR-P and Group SFR-B (p > 0.05).</p><p><strong>Conclusion: </strong>The brushing motion followed by the pecking motion did not cause microcracks. None of the file systems examined in the study induced microcracks.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"173"},"PeriodicalIF":3.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Radiation-induced changes in salivary metabolite profile and pathways in head and neck cancer patients.","authors":"Saga Ramsay, Eelis Hyvärinen, Wilfredo González-Arriagada, Tuula Salo, Marcio Ajudarte Lopes, Jopi J W Mikkonen, Bina Kashyap, Arja M Kullaa","doi":"10.1007/s00784-025-06251-2","DOIUrl":"10.1007/s00784-025-06251-2","url":null,"abstract":"","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"176"},"PeriodicalIF":3.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence and risk factors affecting fibrinolytic alveolitis after permanent tooth extractions.","authors":"Anwar B Anwar Bataineh, Saleh T Alkadi","doi":"10.1007/s00784-025-06257-w","DOIUrl":"10.1007/s00784-025-06257-w","url":null,"abstract":"<p><strong>Objectives: </strong>As a result of the blood clot breaking down or vanishing, a condition can occasionally arise following tooth extraction, especially after traumatic extraction, that leaves the exposed bone in the socket looking dry. The aim of this study was to investigate the incidence and risk factors associated with fibrinolytic alveolitis following extraction of permanent teeth in Jordan.</p><p><strong>Materials and methods: </strong>Data were collected over a period of 6 months from the Dental Management System for every patient who had permanent teeth extraction at the Oral Surgery Clinic, Faculty of Dentistry/ Jordan University of Science and Technology. Data were age, sex, systemic status of patients, smoking habits, indication of extraction, anatomical location of the extracted teeth was divided into: Upper teeth and lower teeth, number of tooth/teeth extracted, procedure involved in teeth extraction, complications during extraction, compliance to post extraction instructions, fibrinolytic alveolitis present.</p><p><strong>Results: </strong>During six-month period from October 2022 to March 2023 a total of 480 permanent teeth extractions of 316 patients, of which 204(64.56%) male patients and 112(35.44%) female patients, the ratio of male: female was1.8:1. Out of all 480 extractions the incidence of fibrinolytic alveolitis was 22(4.58%) of all extractions and 6.96% of affected patients.</p><p><strong>Conclusions: </strong>The overall incidence of fibrinolytic alveolitis was comparable to that of previous research. The findings also suggest that the method and location of extraction are important factors in the development of fibrinolytic alveolitis.</p><p><strong>Clinical relevance: </strong>Even though general dental offices often treat patients with fibrinolytic alveolitis, oral and maxillofacial surgeons play a critical role in treatment and achieving better results. The current theories around the prevention and treatment of fibrinolytic alveolitis, as well as the complexity and numerous natures of the etiology of fibrinolytic alveolitis, should be known to clinicians.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"175"},"PeriodicalIF":3.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, assessment and treatment of molar incisor hypomineralisation (MIH) among German dentists.","authors":"Carla Ostermann, Christian Splieth, Mohammad Alkilzy","doi":"10.1007/s00784-025-06249-w","DOIUrl":"10.1007/s00784-025-06249-w","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the knowledge, perceptions, and clinical practices of German dentists regarding molar incisor hypomineralisation (MIH), a significant issue in paediatric dentistry. To date, no study has examined the knowledge and experience of German dentists with MIH.</p><p><strong>Subjects and methods: </strong>A digital questionnaire comprising 25 items was designed to assess German dentists' understanding of MIH. The questionnaire was available online via Survio.de from March to June 2023. The responses were entered anonymously into an Excel spreadsheet and analysed using SPSS 29.0.</p><p><strong>Results: </strong>625 dentists were surveyed, and 517 questionnaires were evaluated. 99.2% of respondents treated patients with MIH, and 92.5% considered it a significant clinical problem. Commonly observed clinical presentations included yellow/brown defects (81%), enamel loss (46%) and white defects (36.4%). 68.6% of respondents noted an increase in MIH prevalence. Treatment barriers included child behaviour (44.7%), difficulties with local anaesthesia (22.7%), and extended treatment duration (15.3%). Most dentists (77.8%) expressed a desire for further training on MIH.</p><p><strong>Conclusions: </strong>The data indicated that dentists' MIH management was influenced by specialisation and diagnostic confidence. Despite basic knowledge and experience, many respondents expressed a need for further education. Age-related biases and limited awareness of new techniques highlight the need for further research.</p><p><strong>Clinical relevance: </strong>Dentists must receive comprehensive training to enable them to diagnose and treat patients promptly. This should include the development of updated courses, which should be aligned with international guidelines.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"171"},"PeriodicalIF":3.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress distribution in the closure of anterior maxillary diastemas using different restorative approaches: a finite element analysis.","authors":"Kevser Karakaya, Rahime Zeynep Erdem","doi":"10.1007/s00784-025-06258-9","DOIUrl":"10.1007/s00784-025-06258-9","url":null,"abstract":"<p><strong>Objective: </strong>This finite element analysis study aims to evaluate the effects of restoring 1 mm and 2 mm diastemas in maxillary anterior incisors with different CAD-CAM materials on stress distribution and to compare full crown and laminate veneer restorations.</p><p><strong>Materials and methods: </strong>Maxillary anterior tooth models simulating laminate veneer and full crown restorations were created. The experimental groups were categorized into four based on diastema width (1 mm and 2 mm) and CAD-CAM materials. Loads of 50 N, 150 N, and 250 N were applied vertically at 0° and obliquely at 30° and 60° angles to the central incisor's incisal midpoint. Von Mises stress values were analyzed numerically and visually with color-coded comparisons for all models.</p><p><strong>Results: </strong>When laminate veneer restorations were used to close diastemas, the highest stress values under a 250 N vertical load and 2 mm diastema were observed in the lateral incisor. IPS E.max exhibited a stress value of 166.88 MPa for the superstructure, while Lava Ultimate recorded 35.49 MPa in the lateral incisor. Within the dentin, the highest stress was 45.92 MPa for IPS E.max and 65.74 MPa for Lava Ultimate in the lateral incisor. When full crown restorations were employed, the highest stress values under a 250 N vertical load were again recorded in the lateral incisor. IPS E.max showed a stress value of 198.03 MPa for the superstructure, while Lava Ultimate demonstrated 40.80 MPa. The highest stress values within the dentin were 65.20 MPa for IPS E.max and 65.74 MPa for Lava Ultimate in the lateral incisor.</p><p><strong>Conclusions: </strong>This finite element analysis highlights that the type of restoration and diastema width significantly affect stress distribution in anterior teeth. IPS E.max material exhibited higher stress values, leading to more significant stress accumulation than other materials in laminate veneer and full crown restorations. Additionally, Lava Ultimate showed the highest stress values within the dentin. These findings underscore the importance of selecting appropriate CAD-CAM materials and restoration types to optimize stress distribution in diastema closures, providing critical insights for restoration planning.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"172"},"PeriodicalIF":3.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whitening efficacy and removal of extrinsic tooth stain of sodium phytate-containing whitening toothpaste: a randomized controlled trial.","authors":"Tianqiang Cui, Zhuoying Liu, Yina Cao, Qiqiu Wang, Lifen Li, Fengjuan Wu, Qinghui Zhi, Liangyue Pang","doi":"10.1007/s00784-025-06248-x","DOIUrl":"10.1007/s00784-025-06248-x","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to evaluate the whitening efficacy and removal of extrinsic tooth stain of sodium phytate-containing whitening toothpaste, as well as to evaluate participant satisfaction.</p><p><strong>Methods: </strong>A total of eighty participants were recruited for this randomized, triple-blind controlled trial and were randomly assigned to either the test group (toothpaste containing 0.875% sodium phytate, ST) or the control group (toothpaste without sodium phytate, CT). Tooth color was assessed using a VITA spectrophotometer and changes in exogenous tooth stains were evaluated using the Lobene Index, which were conducted at baseline, as well as at 2, 3, and 4 weeks. Additionally, participants' self-assessment of tooth color were evaluated through an aesthetic numerical analogue scale. Statistical tests were conducted appropriately, and the significance level was established at α = 0.05.</p><p><strong>Results: </strong>This study demonstrates that the b-value of the index teeth in the ST group was significantly lower (p < 0.05) than that of the CT group, while the △WI_D values were significantly higher (p < 0.05) at both the 3-week and 4-week time points. Additionally, the a-value of the index teeth in the ST group was significantly lower (p < 0.05) compared to the CT group, and the L-value of the index teeth in the ST group was significantly higher (p < 0.05) than that of the CT group after 4 weeks. The differences in color between the two treatments, as analyzed by the CIELAB (ΔE), were not statistically significant. Similarly, no significant differences were observed in the changes of the Lobene Index or in participant satisfaction regarding tooth color between the two groups. A comparative analysis of the subjects in the ST group at the 3-week and 4-week marks revealed that the L-value of the index tooth at 4 weeks was significantly higher than that at 3 weeks (p < 0.001), while the b-value was significantly lower at 4 weeks compared to 3 weeks (p < 0.001).</p><p><strong>Conclusion: </strong>Brushing teeth with sodium phytate-containing whitening toothpaste for a duration of three weeks demonstrates notable whitening effects, which significantly increase over time. However, after four weeks, the sodium phytate-containing toothpaste shows minimal efficacy in removing extrinsic tooth stains.</p><p><strong>Clinical significance: </strong>Sodium phytate may be incorporated into toothpaste as a whitening agent.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"170"},"PeriodicalIF":3.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative in vitro study on monomer release from flash-free or conventional bonding systems.","authors":"Baris Can Telatar, Gul Yildiz Telatar","doi":"10.1007/s00784-025-06250-3","DOIUrl":"10.1007/s00784-025-06250-3","url":null,"abstract":"<p><strong>Objectives: </strong>To examine the amount of residual monomer released from adhesive-precoated flash-free brackets and compare it to traditional light cure and chemical cure adhesives.</p><p><strong>Materials and methods: </strong>Sixty stainless steel brackets were bonded on upper premolar teeth using three groups of adhesive systems: Adhesive-precoated flash-free system, Transbond XT light cure, Sia chemical cure. The extracts of 0.5 ml of liquid samples were taken on the first, 7th, 21st, and 35th days. To determine the amount of residual monomer release, extracted samples were analyzed using a liquid chromatography device.</p><p><strong>Results: </strong>Urethane-dimethacrylate, triethylene glycol dimethacrylate, hydroxyethyl methacrylate and Bisphenol A-glycidyl methacrylate monomers were released from all study groups. The amount of total monomer release except hydroxyethyl methacrylate was detected as greater in adhesive-precoated flash-free system. Among the study groups, the chemical cure group showed the highest hydroxyethyl methacrylate monomer release.</p><p><strong>Conclusions: </strong>This in vitro study indicate that the APC flashless system exhibited the greatest release of residual monomers, suggesting that its possible cytotoxic effects need to be evaluated prior to clinical application.</p><p><strong>Clinical relevance: </strong>This is the first study that evaluates the residual monomer release from adhesive-precoated flash-free brackets. The novel bracket system has a different mesh structure at the bracket base compared to traditional systems with different chemical components which might alter the amount of residual monomer release. To investigate the harmful effects of adhesives used in orthodontic applications is very important for the health of the patient and the orthodontist.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"169"},"PeriodicalIF":3.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral health in adolescents: periodontal inflammatory biomarkers during orthodontic clear aligner therapy.","authors":"Carolin Kredig, Eva Peuckert, Irene Schmidtmann, Thomas Drechsler, Christina Erbe","doi":"10.1007/s00784-025-06212-9","DOIUrl":"10.1007/s00784-025-06212-9","url":null,"abstract":"<p><strong>Objectives: </strong>This prospective study aimed to evaluate periodontal inflammation in adolescents undergoing orthodontic treatment with clear aligners (Invisalign® Teen, Align Technology, San Jose, CA, USA). Key objectives included assessing the presence of 11 periodontitis-associated marker bacteria, active matrix metalloproteinase-8 concentrations in sulcular fluid, and the influence of IL-1 polymorphism genotypes on periodontal health.</p><p><strong>Materials and methods: </strong>Fifty adolescent patients (13.3 ± 1.8 years) with mixed and permanent dentition participated. Gingival crevicular fluid samples were analyzed at multiple time points: before, during, and one year after aligner treatment. Periodontal health was assessed using the Gingivitis Index and the Modified Quigley-Hein Index. Genotypic analysis of IL-1 polymorphism was also performed. Statistical analyses included mixed linear models and generalized linear models to explore correlations.</p><p><strong>Results: </strong>All combinations of IL-1 polymorphism genotypes were found in the sample. No significant increase in periodontal inflammation or aMMP-8 concentrations was observed over the treatment period. Marker bacteria from the red and orange-associated complexes remained at low levels, while significant changes occurred in the orange and green complexes, particularly Capnocytophaga spp. (p = 0.0042) and Fusobacterium spp. (p = 0.0365). GI correlated significantly with aMMP-8 levels (p = 0.0017), but no genotype effect on GI was observed. MQH showed associations with pathogens from the orange and green complexes, including Capnocytophaga spp. and Fusobacterium spp.</p><p><strong>Conclusions: </strong>Clear aligner treatment in adolescents, including those with an unfavorable genotype, does not increase periodontal inflammation when accompanied by good oral hygiene.</p><p><strong>Clinical relevance: </strong>Regular periodontal monitoring and hygiene reinforcement is important during orthodontic treatment, especially in adolescent patients.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"168"},"PeriodicalIF":3.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional long-term assessment of soft tissue stability after orthognathic mandibular advancement for class II malocclusion correction.","authors":"Alexandre A Franco, Lucia Helena S Cevidanes, Leonardo Koerich, Luiz Carlos F Silva, Rhita Cristina C Almeida, Felipe de Assis R Carvalho, Cátia Cardoso A Quintão, Marco Antonio O Almeida","doi":"10.1007/s00784-025-06237-0","DOIUrl":"10.1007/s00784-025-06237-0","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to assess the stability of soft tissues one and three years after orthognathic mandibular advancement surgery by superimposing the cone-beam computed tomography (CBCT) images and quantifying potential soft tissue changes following surgery.</p><p><strong>Materials and methods: </strong>In this prospective observational study, changes in the hard and soft tissues of the chin, lower incisors, and lower lip due to displacement and/or bone remodeling were analyzed using three-dimensional models based on CBCT superimposition images acquired before surgery (T0), one year after surgery (T1), and three years after surgery (T3). The cranial base was used as a reference for the superimpositions, which were based on fully automated grayscale voxel-wise registrations.</p><p><strong>Results: </strong>The soft chin showed more than 2 mm displacement between T0 and T1 and between T0 and T3, along with hard chin displacement. The lower lip had less displacement at T0 and T1. The soft tissue adaptation between T1 and T3 indicates stability of the soft tissue correction.</p><p><strong>Conclusion: </strong>Even though the soft tissue three-dimensional response one year after surgery was variable, the investigated anatomical regions were stable between one- and three years post-surgery, and only the soft chin exhibited some relapse. The soft tissue chin changes observed were strongly correlated with the hard chin.</p><p><strong>Clinical relevance: </strong>This study provides comprehensive and long-term insights considering soft tissue facial features' stability after orthognathic surgery, impacting orthodontic treatment planning, and integrating both problem-oriented and goal-oriented approaches. The outcomes emphasize the importance of surgical aesthetics alongside occlusion, ultimately contributing to better long-term results and enhanced patient satisfaction.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"167"},"PeriodicalIF":3.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between REDOX enzymes and NET markers expression during the development of periapical lesions in mice.","authors":"Clara Marina Pereira Cavalcanti Silva, Vivian Vicentin Massoni, Lisa Danielly Curcino Araujo, Ricardo Barbosa Lima, Aluísio Eustáquio de Freitas Miranda-Filho, Carolina Maschietto Pucinelli, Marília Pacífico Lucisano, Paulo Nelson-Filho, Alberto Consolaro, Raquel Assed Bezerra da Silva, Léa Assed Bezerra da Silva","doi":"10.1007/s00784-025-06246-z","DOIUrl":"10.1007/s00784-025-06246-z","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate and correlate the expression of REDOX enzymes and NET markers during the development of periapical lesions (PLs) in mice.</p><p><strong>Methods: </strong>PLs were experimentally induced by pulpal exposure of first molars. In all, 42 mice were involved in a negative control and five periods (after 2, 5, 7, 21 and 42 days) (n = 7 in each period). Microscopic analysis of the specimens was carried out, considering inflammatory response and the PL area (mm²). In addition, qRT-PCR (genes) and immunohistochemistry assay (proteins) were performed for REDOX enzymes (NOS2, SOD1, CAT, and GSR) and NET markers (MPO, H3CIT, and ELANE). The significance level was set at 5%.</p><p><strong>Results: </strong>Pulpal exposure led to the formation of PLs, especially after 21 and 42 days, when there was significantly increased bone resorption compared to previous periods (P < 0.001). There was an increased expression of all REDOX enzymes, as well as MPO and H3CIT markers after pulpal exposure in at least one time point in relation to the negative control, especially after 5 days (P = 0.002 or less). NOS2, SOD1, ELANE and H3CIT were significantly correlated with the PL area (P < 0.05). In addition, there was also a correlation between REDOX enzymes and NET markers (P < 0.05), such as all REDOX enzymes with H3CIT, CAT and GSR with MPO, and CAT and GSR with ELANE (P < 0.05).</p><p><strong>Conclusions: </strong>Pulpal exposure modified the expression of REDOX enzymes and NET markers throughout the development of PLs, as well as some of these enzymes and markers were correlated, which may suggest the association of different molecular pathways.</p><p><strong>Clinical relevance: </strong>The association between REDOX activity and NETs, including their molecular signaling, may modify the development of PLs after pulpal exposure and should be investigated as a possible therapeutic target in periapical bone repair.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 3","pages":"166"},"PeriodicalIF":3.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}