Clinical Oral Investigations最新文献

{"title":"Cystic fibrosis and its impact on the oral cavity and dental treatments - a scoping review.","authors":"Inês Lopes Cardoso, Maria Inês Guimarães, Elisa Le Cozic, Fernanda Leal","doi":"10.1007/s00784-026-07137-7","DOIUrl":"10.1007/s00784-026-07137-7","url":null,"abstract":"<p><strong>Objectives: </strong>Cystic fibrosis is an autosomal recessive disease caused by mutations in the CFTR gene, mainly affecting the respiratory and digestive systems. Advances in diagnosis and treatment have increased life expectancy, making cystic fibrosis a chronic condition requiring long-term multidisciplinary care. Consequently, oral health has gained importance, as disease manifestations and prolonged therapies may influence the oral cavity and dental management. This review aimed to analyse the available scientific evidence regarding the impact of cystic fibrosis on oral health and dental treatments. The guiding research question was: What is the impact of cystic fibrosis on the oral cavity and dental treatments?</p><p><strong>Methodology: </strong>A literature search was conducted in PubMed, Scopus, Web of Science and Cochrane Library using the keywords: cystic fibrosis, oral health, and dental treatment. After applying inclusion and exclusion criteria, 22 articles were selected.</p><p><strong>Results: </strong>Although findings remain heterogeneous, the included studies suggest that cystic fibrosis is associated with specific oral changes. In children, a lower prevalence of dental caries was frequently reported, often linked to prolonged antibiotic use. In adults, studies indicated a higher prevalence of untreated caries and poorer oral health status. Developmental enamel defects and salivary alterations were also commonly observed.</p><p><strong>Conclusions: </strong>Cystic fibrosis may significantly affect oral health, highlighting the need for individualized dental care focused on prevention, regular monitoring, and adaptation to each patient's specific needs.</p><p><strong>Clinical relevance: </strong>Individualized preventive and therapeutic strategies, regular monitoring, and integration of dental professionals into multidisciplinary teams may improve oral health outcomes, treatment adherence, and quality of life.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of 3D superimposition software on intraoral scanner accuracy and monitoring outcome.","authors":"Albert Mehl, Jenny Buhl, Andreas Ender","doi":"10.1007/s00784-026-07136-8","DOIUrl":"10.1007/s00784-026-07136-8","url":null,"abstract":"<p><strong>Objectives: </strong>To determine whether 3D superimposition software (\"Apps\") systematically affects 3D deviation outcomes derived from intraoral scanner (IOS) data, using two quantile‑range accuracy metrics (Q90_Q10_half and Q95_Q05_half).</p><p><strong>Materials and methods: </strong>Two jaw‑sized reference models were digitized with a high‑resolution optical scanner (ATOS III) to generate reference STL meshes (REF_STL). Five IOS devices acquired ten scans per device and per jaw model, exported as STL meshes (IMP_STL): Emerald Green, iTero 5D, Medit i900, Primescan, and Trios 5. Each IMP_STL was trimmed (1 mm above gingival line) and superimposed to REF_STL in four Apps: commercial industrial software: Geomagic Control X and GOM Inspect, and open dentistry-focused software Medit Design (Compare) and Dentexion; resulting in total to N = 400 superimpositions. For each superimposition, Q90_Q10_half and Q95_Q05_half values were computed from point-wise signed surface distances. A linear mixed‑effects model tested factor App as fixed effect with random intercepts for factors Device and Jaw, followed by Bonferroni‑adjusted pairwise comparisons (α = 0.05).</p><p><strong>Results: </strong>App significantly influenced Q90_Q10_half (p < 0.01) and Q95_Q05_half (p < 0.001). Control X yielded higher adjusted means than Dentexion and Compare for Q90_Q10_half (both p < 0.01), while Dentexion, GOM, and Compare did not differ. For Q95_Q05_half, Control X exceeded all other Apps (all p < 0.01). Notably, IOS rankings were stable across Apps, indicating that software altered magnitude but not relative ordering.</p><p><strong>Conclusions: </strong>Evaluation software is a significant source of variability in 3D deviation metrics; Control X produced consistently higher (more conservative) quantile‑range values, particularly for Q95_Q05_half.</p><p><strong>Clinical relevance: </strong>Inter‑study comparisons in digital accuracy research should report the evaluation software and settings. Dentistry‑focused Apps (e.g., with automated tooth segmentation) may reduce operator‑dependent effects and facilitate clinically applicable monitoring.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of two biomimetic remineralizing systems on artificially demineralized enamel: Assessment of mineral recovery and orthodontic bracket bond strength.","authors":"Ascensión Vicente, Antonio J Ortiz-Ruiz, Clara Serna-Muñoz, Inmaculada Cabello, Yolanda Martínez-Beneyto","doi":"10.1007/s00784-026-07130-0","DOIUrl":"10.1007/s00784-026-07130-0","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the effect of two remineralizing systems (Regenerate™ and Orthocare™) on the mineral recovery of demineralized enamel and on the shear bond strength (SBS) of brackets.</p><p><strong>Methods: </strong>180 bovine teeth were divided into four groups: 1)Intact enamel, 2)Demineralized enamel, 3)Demineralized enamel/Regenerate™, and 4)Demineralized enamel treated/Orthocare™. Groups 2 to 4 were demineralized. All samples were brushed three times daily; groups 1 and 2 without toothpaste, and groups 3 and 4 were treated with Regenerate™ or Orthocare™. Outcomes were assessed by SBS, adhesive remnant area, field-emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Raman spectroscopy, surface microhardness, and micro-computed tomography (micro-CT). Parametric or non-parametric tests according to data distribution (α = 0.05).</p><p><strong>Results: </strong>Demineralized enamel showed significantly lower SBS, adhesive remnant, surface microhardness, mineral density, calcium and phosphorus content, and phosphate-related Raman parameters than intact enamel (p < 0.05). FE-SEM revealed increased porosity. Neither remineralizing system significantly restored SBS compared with untreated demineralized enamel. However, both treatments promoted partial enamel remineralization, improved calcium (Ca) and phosphorus (P) levels (p < 0.001), increasing surface microhardness and mineral density (p < 0.05). Raman showed greater recovery of phosphate (I₉₆₀) and carbonate (I₁₀₇₀) peaks in the Regenerate™ group, indicating better mineralization and crystallinity recovery than Orthocare™.</p><p><strong>Conclusions: </strong>Both agents partially remineralized enamel without fully restoring sound enamel properties. Regenerate™ demonstrated better mineral recovery than Orthocare™. However, the newly formed hydroxyapatite appeared immature, and bond strength was not significantly improved compared with demineralized enamel.</p><p><strong>Clinical relevance: </strong>Remineralizing agents may improve demineralized enamel before orthodontic bonding without significantly affecting bracket bond strength.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Developmental enamel defects and hypomineralized second primary molars among preschool children in Kuwait: associations with caries severity and oral health-related quality of life.","authors":"Abrar Alanzi, Sahar Behzadi, Jagan Baskaradoss, Aishah Alsumait","doi":"10.1007/s00784-026-07139-5","DOIUrl":"10.1007/s00784-026-07139-5","url":null,"abstract":"","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148988451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Helicobacter pylori: a scoping review and pilot study on the PCR-culture detection paradox.","authors":"Erin Miller, Jasmin Pyu Pyu Wai Htun, Julie-Anne Martis, Kausar Sadia Fakhruddin, Eng Chua, Alfred Tay, Hooi Ee, Erwin A Paz, Victor Haruo Matsubara","doi":"10.1007/s00784-026-07134-w","DOIUrl":"10.1007/s00784-026-07134-w","url":null,"abstract":"<p><strong>Objective: </strong>The role of the oral cavity as a reservoir for Helicobacter pylori remains controversial. This hybrid study, combining a scoping review and a pilot clinical study, aimed to synthesise global evidence on oral H. pylori detection and investigate the discrepancy between molecular and culture-based findings.</p><p><strong>Materials and methods: </strong>A scoping review following JBI and PRISMA-ScR guidelines identified human studies reporting oral H. pylori detection using molecular and/or culture-based methods. Review findings guided a pilot clinical study in which supragingival plaque and oral mucosal swabs were collected from symptomatic and asymptomatic H. pylori-positive individuals. Detection was performed using nested PCR (16 S rRNA and ureA) and selective microaerophilic culture.</p><p><strong>Results: </strong>Thirty-nine studies involving over 3,000 participants were included. Molecular detection rates varied widely (0-90%), while culture-confirmed viable H. pylori was exceedingly rare and restricted to protected anaerobic oral niches. In the pilot study (n = 8), PCR detected H. pylori DNA in 50% of participants, with higher detection in mucosal swabs (37.5%) than supragingival plaque (25%). No viable organisms were recovered by culture, which was dominated by commensal oral species.</p><p><strong>Conclusion: </strong>Both the scoping review and pilot study consistently demonstrated a PCR-positive/culture-negative pattern for oral H. pylori. These findings suggest that oral detection likely reflects transient bacterial presence rather than stable colonisation. Future studies should employ multi-gene molecular assays, targeted sampling, viability-based techniques, and gastric-oral strain comparisons to clarify the biological significance of oral H. pylori detection.</p><p><strong>Clinical relevance: </strong>Although H. pylori DNA is frequently detected in oral samples, viable organisms are rarely identified. Oral PCR positivity should therefore not be interpreted as active infection, and clinical management should continue to rely on established gastric diagnostic pathways.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13585946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148988406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial effect of treating thermoformable cellulose-based materials with essential oils.","authors":"Lisa Stäuble, Olivier Braissant, Carlalberta Verna, Michael M Bornstein, Monika Astasov-Frauenhoffer","doi":"10.1007/s00784-026-07111-3","DOIUrl":"10.1007/s00784-026-07111-3","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the antimicrobial effect of essential oil-loaded thermoformable cellulose-based material on the formation of biofilms by Streptococcus mutans and three-species biofilms (Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus sanguinis) under in vitro flow conditions.</p><p><strong>Materials and methods: </strong>Cellulose-based thermoformable materials were pre-treated with either essential oils, or phosphate-buffered saline (control). The materials were combined with saliva-treated hydroxyapatite (HA) discs in a flow chamber system. S. mutans and a three-species periodontal biofilm were cultivated under aerobic and anaerobic flow conditions for 16 h. Biofilm formation was quantified using crystal violet staining and antimicrobial activity was assessed via isothermal microcalorimetry (IMC).</p><p><strong>Results: </strong>Addition of essential oils reduced S. mutans biofilm formation by 34% on HA and by 52.5% on cellulose-based material. IMC showed reduced growth rate and total metabolic activity and prolonged lag phase for periodontal pathogens. However, mixed-species periodontal biofilms exhibited minimal changes (-6.3% on HA and + 12% on cellulose-based material).</p><p><strong>Conclusions: </strong>In-vitro data shows that treating cellulose-based materials with essential oils can significantly reduce the formation of S. mutans biofilms, suggesting potential benefits for preventing caries for example during orthodontic therapy by using aligners. However, no significant inhibitory effect was observed against periodontal biofilm, indicating the need for further optimisation of antimicrobial combinations regarding this purpose.</p><p><strong>Clinical relevance: </strong>The findings provide preclinical evidence that essential oil-loaded thermoformable dental materials reduce biofilm accumulation and provide basis for further evaluation for applications in oral medicine, cariology, and orthodontics. Clinical studies are required to confirm their preventive efficacy and relevance in vivo.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13582289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of periodontally accelerated osteogenic orthodontics on treatment duration and periodontal outcomes: a systematic review and meta-analysis.","authors":"Han Xu, Kaile Sheng, Yan Wang, Mengyao Ding, Kun Wang, Xiaohang Xu, Chris Tat Chuen Fok, Qinzi Xie, Yunnan Jin, Chuyi Jin, Yifan Xie, Lipei Liu, Xing Chen, Cheng Ding","doi":"10.1007/s00784-026-07133-x","DOIUrl":"10.1007/s00784-026-07133-x","url":null,"abstract":"<p><strong>Objectives: </strong>This review examined whether periodontally accelerated osteogenic orthodontics (PAOO) reduces orthodontic treatment time and whether it alters periodontal outcomes, including alveolar bone thickness, root length, and soft-tissue measures.</p><p><strong>Materials and methods: </strong>PubMed, Embase, Web of Science, Google Scholar, the Cochrane Library, and ClinicalTrials.gov were searched through June 3, 2026. Studies comparing PAOO with conventional unassisted orthodontic treatments with relevant clinical or radiographic outcomes were included. Two reviewers independently extracted data and assessed risk of bias using ROBINS-I, while the certainty of evidence was rated with GRADE. Comparable continuous outcomes were pooled as mean differences.</p><p><strong>Results: </strong>Eleven non-randomized studies were included. PAOO was associated with a shorter treatment duration (MD = -7.51 months, 95% CI = -10.42 to -4.59, p < 0.001), albeit with high heterogeneity (I² = 90%). Buccal bone thickness increased significantly across all vertical levels (MD range: 0.60 to 1.10 mm, all p < 0.05). Lingual bone thickness was significantly greater only at 4 mm (MD = 0.23 mm, 95% CI = 0.05 to 0.40, p = 0.01) and 6 mm (MD = 0.34 mm, 95% CI = 0.04 to 0.64, p = 0.03) apical to the cementoenamel junction. Neither keratinized gingival width nor root length differed significantly between groups.</p><p><strong>Conclusions: </strong>PAOO may shorten treatment and increase buccal bone thickness, but evidence of lingual bone gain was restricted to selected measurement sites. Keratinized gingival width and root length did not differ significantly between groups. Because all included studies were non-randomized and the certainty of evidence ranged from very low to low, these findings should be interpreted cautiously. Well-designed randomized trials with longer follow-up are needed.</p><p><strong>Clinical relevance: </strong>PAOO may represent a potential treatment option for selected adult patients, particularly those undergoing anterior decompensation or camouflage treatment with thin buccal bone, but the evidence remains limited.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of tooth preparation design on fracture load and fracture behavior of deciduous crowns fabricated by subtractive and additive manufacturing.","authors":"Misaki Hamashima, Kanae Wada, Shinya Oishi, Hidekazu Takahashi, Kaori Kohi, Daiki Kondo, Yujeong Shin, Yuika Yamanaka, Atsushi Oishi, Asuna Sugimoto, Kanako Kishima, Yumiko Nakashima, Rika Kurogoshi, Tsutomu Iwamoto","doi":"10.1007/s00784-026-07127-9","DOIUrl":"10.1007/s00784-026-07127-9","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated the effects of crown material and margin design on fracture load and behavior of computer-aided design/computer-aided manufacturing (CAD-CAM) crowns for deciduous molars.</p><p><strong>Methods: </strong>Three subtractive-manufacturing (SM) materials and two additive-manufacturing (AM) materials were evaluated. Crowns with knife-edge, chamfer, and deep chamfer margins were fabricated and bonded to standardized deciduous molar abutments (n = 12/group; total = 180). Fracture load was measured under static loading, and fracture modes were classified by repairability and abutment involvement. Fracture surfaces and crack propagation were examined by scanning electron microscopy.</p><p><strong>Results: </strong>Significant effects of material and material-margin interactions were observed (p < 0.05). ESTELITE BLOCK II exhibited the highest fracture load with knife-edge margins, whereas Detax showed the highest values with deep chamfer margins. However, non-repairable abutment-involving fractures occurred in both materials with knife-edge margins. CERASMART PRIME showed lower fracture load but mainly repairable fractures. SHOFU BLOCK HC and CERASMART PRIME, unified crown-abutment fracture in ESTELITE BLOCK II, interlayer delamination in Detax, and superficial radial cracking in NextDent.</p><p><strong>Conclusions: </strong>The effects of margin design on fracture load and fracture behavior were material-dependent. Among subtractive-manufacturing materials, fracture load was generally consistent with reported mechanical properties, whereas fracture behavior in additive-manufacturing materials may have been influenced by internal structure and build orientation. Material selection should consider fracture load, fracture severity, and abutment involvement.</p><p><strong>Clinical relevance: </strong>Material and margin selection influence fracture resistance and failure patterns. Fracture severity and abutment involvement should be considered in addition to fracture load.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13582367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interdental brushing behaviour in adults: Influence of periodontal status and socioeconomic factors.","authors":"Pasquale Santamaria, Francesco Fanelli, Rohan Mangalpara, Thamara Kumar, Sarah Van Der Lith, Giuseppe Troiano, Luigi Nibali","doi":"10.1007/s00784-026-07129-7","DOIUrl":"10.1007/s00784-026-07129-7","url":null,"abstract":"<p><strong>Aim: </strong>To evaluate patterns of interdental brush (IB) use across different stages and grades of periodontitis and to examine associations with socioeconomic, demographic, and behavioural factors.</p><p><strong>Methods: </strong>This cross-sectional study included 375 adults referred for secondary periodontal care at Guy's and St Thomas' Hospital (London, UK). Periodontal status was classified using the 2018 periodontal classification. Participants completed a 10-item questionnaire on IB use and perceived barriers. Area-level household income was estimated using postcode-linked national statistics. Associations were analysed using chi-squared tests and multivariable logistic and ordinal regression models.</p><p><strong>Results: </strong>Daily IB use (63.0%) was significantly associated with periodontitis stage (p<0.001) but not grade. Patients with Stage III-IV periodontitis had higher odds of daily IB use (OR=3.11, 95% CI 1.88-5.15, p<.001). Higher income and older age were positively associated with IB use, while current smoking was a strong negative predictor (OR=0.31, 95% CI 0.15-0.67). Common barriers included pain, discomfort, and cost.</p><p><strong>Conclusions: </strong>A distinct profile of periodontal patients who are less likely to use IBs regularly was characterised by early-stage disease, younger age, lower income, and current smoking status. Dissatisfaction driven by practical, financial, and environmental concerns highlights the need for tailored preventive advice and personalised interdental cleaning strategies to improve adherence.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13582308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profile of the dentine pellicle modified with plant polyphenols and fluoride.","authors":"Thiago Saads Carvalho, Tommy Baumann, Marilia Afonso Rabelo Buzalaf, Daniela Rios, Matthias Hannig, Samira Helena Niemeyer","doi":"10.1007/s00784-026-07097-y","DOIUrl":"10.1007/s00784-026-07097-y","url":null,"abstract":"<p><strong>Objectives: </strong>Despite its protective role, the dentine pellicle has rarely been studied, therefore we aimed to map out the proteomic profile of in vitro dentine pellicles before and after modification.</p><p><strong>Materials and methods: </strong>A total of 135 human dentine specimens were prepared. After initial pellicle formation with 150 µl pooled human saliva (37 °C, 30 min), the dentine specimens were immersed in one of 9 pellicle modification solutions (2 ml/specimen): deionized water (non-modified pellicle), SnCl₂/NaF/AmF (commercial solution containing 800 ppm Sn²⁺ and 500 ppm F^-), NaF solution (500 ppm F^- ), and six polyphenol solutions (2 mg / ml) with or without 500 ppm F^-: blueberry extract (BBE and BBE + F^-), green tea extract (GTE and GTE + F^-) and grape seed extract (GSE and GSE + F^-). After another aliquot of saliva (150 µl, 37 °C, 60 min), the pellicles were harvested with sodium dodecyl sulphate by rubbing with cotton balls, and taken to proteomic analyses by Liquid Chromatography-Tandem Mass Spectrometry after tryptic digestion.</p><p><strong>Results: </strong>A total of 382 proteins were identified in all the proteomic analyses for all groups. Pellicle modification with fluoride, either NaF or SnCl₂/NaF/AmF, led to the presence of 12 or 14 exclusive proteins, respectively, whereas modification with the solutions containing polyphenols presented less exclusive proteins (4-6 proteins). The number of exclusive proteins was even lower for when polyphenols and fluoride (GTE + F^- and GSE + F^-) were used, with lower abundance of proteases.</p><p><strong>Conclusions: </strong>We conclude that NaF and SnCl₂/NaF/AmF significantly modify the proteome of the dentine pellicle. The combination of fluoride with polyphenols further lowers the abundance of proteins and proteases, which explains the positive effect of these solutions on the dentine pellicles.</p><p><strong>Clinical relevance: </strong>Plant extract solutions with fluoride can significantly modify the proteomic structure of the dentine pellicle, which clarifies the mechanism of action of polyphenols on the protection of dentine demineralization.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13574873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148967094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}