Clinical Oral Investigations最新文献

{"title":"AI-generated 3D models enhance CBCT interpretation of root canal anatomy among undergraduate and postgraduate students.","authors":"Rocharles Cavalcante Fontenele, Airton Oliveira Santos-Junior, João Guilherme Dos Santos Cunha, Thantrira Porntaveetus, Hugo Gaêta-Araujo, Reinhilde Jacobs","doi":"10.1007/s00784-026-06897-6","DOIUrl":"10.1007/s00784-026-06897-6","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the efficacy of artificial intelligence (AI)-driven three-dimensional (3D) anatomical models as an adjunct to cone-beam computed tomography (CBCT) for root canal assessment regarding diagnostic accuracy, observer confidence, and time efficiency among undergraduate and postgraduate students.</p><p><strong>Materials and methods: </strong>In this observational diagnostic study, 26 observers (13 undergraduates and 13 postgraduates) evaluated 22 tooth roots with complex anatomy from nine CBCT scans under two conditions: CBCT alone and CBCT supplemented with AI-generated 3D anatomical models. Observers assessed the number of roots, root canals, and apical foramina, while confidence (5-point Likert scale) and assessment time were recorded. Each observer performed 132 assessments, totaling 3,432 evaluations. A reference standard was established by consensus between two specialists. A significance level was set at 5% (α = 0.05) for all statistical analyses.</p><p><strong>Results: </strong>Augmenting CBCT with AI-generated 3D models significantly improved diagnostic accuracy for all parameters (p < 0.001). Root detection accuracy reached 100% in both groups. Root canal detection increased from 83% to 94% among undergraduates and from 88% to 99% among postgraduates, while apical foramina detection increased to 99% in both groups. Observer confidence significantly increased (p < 0.001), reaching a median score of 5 (IQR: 5-5). Workflow efficiency also improved (p < 0.001), with median assessment time decreasing from 102 s to 39 s for undergraduates and from 97 s to 24 s for postgraduates.</p><p><strong>Conclusion: </strong>AI-driven 3D anatomical models used with CBCT enhance diagnostic accuracy, observer confidence, and evaluation efficiency in endodontic assessment. However, multi-centre studies with larger, more diverse samples, particularly including cases with pronounced artefacts, would further support generalisability.</p><p><strong>Clinical relevance: </strong>AI-generated 3D anatomical models derived from CBCT scans may serve as a valuable adjunct for the interpretation of complex root canal anatomy, improving diagnostic accuracy, increasing observer confidence, and reducing assessment time. These findings support their potential role not only in clinical decision-making but also as an effective educational tool for training dental students and clinicians.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13153009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of antimicrobial photodynamic therapy protocols as adjunct treatment in the management of initial pericoronitis: a randomized, controlled, double-blind clinical trial.","authors":"Tânia Oppido Schalch, Ellen Sayuri Ando-Suguimoto, Bruno Souza Ferreira, Marcia Alves Pinto Mayer, Lara Jansiski Motta, Sandra Kalil Bussadori, Cinthya Cosme Gutierrez Duran, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, Christiane Pavani, Anna Carolina Ratto Tempestini Horliana","doi":"10.1007/s00784-026-06898-5","DOIUrl":"10.1007/s00784-026-06898-5","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to compare the effectiveness of two antimicrobial photodynamic therapy (aPDT) protocols using different methylene blue formulations in the treatment of initial pericoronitis. The research question was whether the new methylene blue formulation provides superior clinical outcomes compared to the conventional formulation.</p><p><strong>Materials and methods: </strong>This randomized, controlled, double-blind clinical trial involved 34 healthy young patients with pericoronitis. The following groups were established: G1 (positive control, n = 17), irrigation with saline solution and aPDT with conventional methylene blue (0.005%, laser λ = 660 nm, 9 J per point, 318 J/cm²); and G2 (experimental, n = 17), using the same therapy but with a patented new formulation of methylene blue. Pain assessed using the Visual Analog Scale (VAS) was defined as the primary outcome. Secondary outcomes include mouth opening, edema, and quality of life (OHIP-14). Microbiological and immunological analyses were performed to complement clinical outcomes. All outcomes were assessed at baseline and on the fourth day after aPDT.</p><p><strong>Results: </strong>Both groups showed statistically significant improvement in pain (G1: p = 0.022; G2: p = 0.001) and mouth opening (G1: p < 0.001; G2: p = 0.002) after treatment. However, no statistically significant differences were observed between the groups in final pain and mouth-opening outcomes (p = 0.845 and p = 0.318, respectively).</p><p><strong>Conclusions: </strong>Within the limitations of this study, both aPDT protocols were associated with improvements in clinical outcomes. No between-group differences were observed in clinical outcomes; differences were limited to microbiological and immunological parameters, with no clinical superiority of the new formulation.</p><p><strong>Clinical relevance: </strong>Both methylene blue formulations may be used as adjunctive treatment options for the management of initial pericoronitis. However, no additional clinical benefit was observed with the new formulation, and these findings do not support a change in current clinical practice.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13152969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro evaluation of the efficacy of a new orthodontic primer in metallic brackets cementation.","authors":"Byron Carpio-Salvatierra, Mayra Alejandra Nuñez, Pâmela Maria Kusdra, Victor Martins Montalli, Ana Del Carmen Armas Vega, Paulo Vitor Farago, Alessandro D Loguercio","doi":"10.1007/s00784-026-06896-7","DOIUrl":"10.1007/s00784-026-06896-7","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy of a new orthodontic primer (Ambar APS Ortho; FGM Dental Products, Joinville, SC, Brazil) on shear bond strength (SBS) and degree conversion (DC) of metallic brackets bonding.</p><p><strong>Materials and methods: </strong>240 sound maxillary premolars were randomized into 24 experimental groups based on: (1) Orthodontic primer (Ambar APS Ortho, Orthoprimer and Transbond XT); (2) Light-curing time (3-seconds and 10-seconds); (3) Light-curing unit (Valo Cordless and Quazar); and (4) Storage condition (immediate time [IT] and after thermocycling [TC]). After each storage time, specimens were subjected to SBS testing at a crosshead speed of 1 mm/min until failure, and values were recorded in MPa. For DC (%) analysis, adhesive discs were prepared and evaluated using micro-Raman spectroscopy at IT only. SBS and DC data were analyzed using four and four-way ANOVA, respectively and Tukey's post hoc test (α = 0.05).</p><p><strong>Results: </strong>Ambar APS Ortho showed significantly higher SBS values across both light-curing units compared to all other groups (p = 0.0001). For DC, Orthoprimer showed the lowest values, while Ambar APS Ortho achieved the highest. Across all primers, a 10 s light-curing exposure resulted in significantly higher DC values compared to 3 s (p < 0.0001).</p><p><strong>Conclusions: </strong>Ambar APS Ortho exhibited superior performance in both SBS and DC, for both 3 s and 10 s light-curing times as well as IT or AT, supporting its reliability for bonding metallic orthodontic brackets.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13153000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of gingival recession type 1 using coronally advanced flap with leucocytes-platelet rich fibrin: a randomized controlled trial.","authors":"Nuno Bernardo Malta Dos Santos, Gustavo Vicentis Oliveira Fernandes, Tiago Marques, Manuel Correia Sousa, André Correia, María Pilar Batalla Vázquez, Juan Blanco-Carrión","doi":"10.1007/s00784-026-06899-4","DOIUrl":"10.1007/s00784-026-06899-4","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the clinical outcomes of gingival recession Type 1 (RT1) treatment using a coronally advanced flap (CAF) alone and in combination with L-PRF at 6 months.</p><p><strong>Materials and methods: </strong>A total of 70 RT1 from 19 patients were included. Participants were randomly assigned to the test group (TG, CAF + L-PRF, n = 42) and the control group (CG) (CAF alone, n = 28). Clinical parameters were assessed at baseline and at 6 months: the primary outcomes were percentage of root coverage (%RC) and complete root coverage (CRC); and the secondary outcomes included mean root coverage (MRC), changes in gingival thickness (GT) and volumetric tissue gain, recession area reduction, analgesic consumption, healing quality (the Inflammatory Proliferative Remodeling [IPR] score), and patient-reported outcome measures (PROMs). Statistical analyses were performed to determine differences between groups; a random-intercept mixed-effects model was used for all site-level outcomes.</p><p><strong>Results: </strong>At six months, %RC was 89.30% ± 20.33% (TG) and 81.60% ± 27.93% (CG) (p > 0.05). For the TG and CG, respectively, %CRC was 73.81% and 57.14%; the mean GT gain was 0.16 ± 0.10 mm and 0.11 ± 0.10 mm (p = 0.08); the mean volume gain was 1.13 ± 1.25 mm³ and 0.86 ± 0.84 mm³ (p = 0.32); the mean number of pills taken was 1.67 ± 0.98 and 2.25 ± 1.02 (p = 0.04); the esthetic satisfaction was 95% ± 5% and 90% ± 7% (p = 0.31); willingness to undergo the procedure again: 93% ± 4% and 88% ± 6% (p = 0.22); sensitivity reduction: 85% ± 6% and 80% ± 8% (p = 0.19). There was no statistically significant difference in healing quality and PROMs.</p><p><strong>Conclusion: </strong>Both CAF alone and CAF combined with L-PRF yielded comparable clinical and volumetric outcomes in RT1 treatments. No statistically significant advantages were observed with the adjunctive use of L-PRF.</p><p><strong>Clinical relevance: </strong>Because CAF alone achieves high predictability for RT1 defects, the routine adjunctive use of L-PRF provides limited additional clinical benefits.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13152877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The outcome of pulp revascularization of necrotic mature permanent teeth with periapical lesions using platelet-rich fibrin versus induced bleeding: a prospective preliminary randomized clinical trial.","authors":"Lama Awawdeh, Bushra Almesleh, Hanan Hammouri, Aladdin Al-Qudah","doi":"10.1007/s00784-026-06877-w","DOIUrl":"https://doi.org/10.1007/s00784-026-06877-w","url":null,"abstract":"<p><strong>Objectives: </strong>The present study evaluated clinically and radiographically the effectiveness of Regenerative endodontic procedures (REPs) using platelet-rich fibrin (PRF) versus induced bleeding (IB) in treating mature necrotic teeth.</p><p><strong>Materials and methods: </strong>Fifty patients with necrotic mature teeth with periapical lesions were randomly divided into two groups, Group 1, IB (n = 25), and Group 2, PRF (n = 25). Treated teeth were assessed clinically and radiographically at 6 and 12 months. Survival rate, success rate, and clinical outcome measures were analyzed.</p><p><strong>Results: </strong>Survival rate was 98% at the 12-month follow-up period, with no significant difference between the groups (P=0.166). A statistically significant increase in periradicular healing was found in both groups at 6 and 12 months, compared to that at baseline (P< 0.001). A significant difference was found in final treatment success between the two groups (p = 0.0219). The IB group achieved a 100% success rate (23/23), while the PRF group showed a 75% success rate (18/24). The overall success rate was 85.4%.</p><p><strong>Conclusions: </strong>All teeth treated with either IB or PRF survived throughout the follow-up period. The preliminary results of this study showed comparable clinical outcomes between the two treatment modalities, although teeth treated with IB showed higher overall success rate than those treated with PRF this should be interpreted within the framework of early phase clinical evidence as the study was not adequately powered to confirm superiority.</p><p><strong>Clinical relevance: </strong>REPs may represent a viable treatment option for mature necrotic teeth, offering a biologically based approach that aims to restore tissue function. Longer follow-up as planned in the second phase of this study, will allow for a more definitive comparison between IB and PRF approaches.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five-year clinical outcomes of root canal treatment using a calcium silicate-based sealer in teeth with apical periodontitis: a retrospective cohort study.","authors":"Filippo Cardinali, Giulia Malvicini, Salvatore Sauro, Mariangela Cernera, Denise Irene Karin Pontoriero, Simone Grandini, Carlo Gaeta","doi":"10.1007/s00784-026-06884-x","DOIUrl":"10.1007/s00784-026-06884-x","url":null,"abstract":"<p><strong>Objective: </strong>Hydraulic calcium silicate-based sealers (CSBSs) are increasingly used in endodontics, but evidence on their long-term clinical effectiveness remains limited. This retrospective study evaluated the 5-year outcomes of primary root canal treatments and retreatments performed with a CSBS in teeth with symptomatic apical periodontitis.</p><p><strong>Materials and methods: </strong>The sample consisted of 637 teeth with symptomatic apical periodontitis treated by a single experienced operator using a premixed CSBS (CeraSeal; Meta Biomed Co., Cheongju, South Korea) with the cold hydraulic condensation technique (CHC). Data were retrospectively collected from clinical charts and radiographic archives. Preoperative variables included sex, systemic health, tooth type, arch location, lesion size, pulpal diagnosis, sinus tract, and preoperative small perforation. Intraoperative factors comprised intracanal medication, apical diameter, obturation length, and sealer extrusion. Postoperative information included the type of coronal restoration. Follow-up evaluations were based on available radiographs and clinical notes at 1, 2, 3, 4, and 5 years. Outcomes were classified according to strict criteria (absence of symptoms and complete radiographic resolution) and loose criteria (absence of symptoms with complete or partial healing). Prognostic factors were investigated using bivariate associations and multivariate logistic regression models.</p><p><strong>Results: </strong>Strict success rates increased progressively (80.2% at 1 year, 85.7% at 2 years, 86.8% at 3 years, 88.2% at 4 years, and 87.67% at 5 years). Loose success remained consistently high (99.2% at 1 year; 90.7% at 4 years, and 87.67% at 5 years). Higher baseline periapical index (PAI) scores significantly reduced odds of strict success at 2, 3, 4 years. Other factors, including age, sex, tooth type, treatment type, extrusion, and restoration, were not significant.</p><p><strong>Conclusions: </strong>CSBSs demonstrated favorable long-term outcomes. Preoperative lesion severity was the main prognostic factor, while demographic and procedural variables showed limited influence on long-term success.</p><p><strong>Clinical relevance: </strong>This study supports the long-term clinical reliability of CSBSs used with CHC in teeth with apical periodontitis. Preoperative lesion severity remains the primary prognostic factor influencing periapical healing over time.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical performance of alkasite and glass-hybrid restorations compared with resin composite in class II cavities: a randomized clinical trial.","authors":"Kemal Işıklı, Uzay Koc Vural, Filiz Yalcin Cakir","doi":"10.1007/s00784-026-06869-w","DOIUrl":"https://doi.org/10.1007/s00784-026-06869-w","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this randomized clinical trial was to evaluate the 18-month clinical performance of alkasite and glass-hybrid restorations compared with resin composite in Class II restorations.</p><p><strong>Materials and methods: </strong>A total of 50 patients requiring at least three Class II restorations in premolar and molar teeth were recruited. Each patient received three restorations, which were randomly assigned to one of the following materials: an alkasite (Cention N, Ivoclar Vivadent), a glass-hybrid (Equia Forte HT, GC Corp.), or a resin composite (Gradia Direct Posterior, GC Corp.). Alkasite and glass-hybrid served as test groups while resin composite served as the control group. During the 18-month follow-up, restorations were scored at baseline, 6, 12, and 18 months using the FDI criteria. Data were analyzed using the Chi-square and Cochran's Q tests (α = 0.05).</p><p><strong>Results: </strong>No significant differences were detected among the groups for esthetic, functional, or biological criteria over 18 months (p > 0.05). Regarding esthetic properties, the control group showed 100% success for all esthetic criteria at all recall visits, while the alkasite group maintained a 96% success rate at all time points, and glass-hybrid showed 98% success at 6 and 12 months and 92% at 18 months. Minor score-2 changes in color match and gloss were detected in the glass-hybrid and alkasite groups but were not significant (p > 0.05). For functional and biological outcomes, all groups achieved 100% success rates for all evaluated parameters.</p><p><strong>Conclusions: </strong>Alkasite and glass-hybrid groups exhibited clinical performance comparable to resin composite over the 18-month follow-up in Class II cavities. All materials demonstrated excellent functional and biological stability while achieving clinically acceptable aesthetic results.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic predictive value of T-stage and lymph node yield in guiding adjuvant radiotherapy decisions for pN1 OSCC without high-risk features: a multi-institutional retrospective cohort study.","authors":"Bo Li, Yu Huang, Hanchen Zhou, Chuanbin Guo, Wei Wei, Wei Cao, Zhien Feng","doi":"10.1007/s00784-026-06894-9","DOIUrl":"https://doi.org/10.1007/s00784-026-06894-9","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to identify a subset of patients with pN1 oral squamous cell carcinoma (OSCC) and no adverse pathological features who derive significant benefit from adjuvant radiotherapy (RT).</p><p><strong>Materials and methods: </strong>In this multicenter retrospective study, 232 eligible pN1 OSCC patients were analyzed. Patients were stratified by T-stage (T1-2 vs. T3-4) and lymph node yield (LNY; high: ≥20; low: <20). The impact of adjuvant RT on survival outcomes was evaluated.</p><p><strong>Results: </strong>A significant survival benefit from adjuvant RT was observed exclusively in the subgroup with advanced T-stage (T3-4) and low LNY (< 20), with improved disease-free survival (69.2% vs. 23.1%, p = 0.003), overall survival (76.9% vs. 38.5%, p = 0.012), and disease-specific survival (80.8% vs. 46.2%, p = 0.014). In contrast, no significant survival differences were found in the other T-stage/LNY subgroups. Furthermore, LNY alone did not independently predict prognosis or RT benefit in the overall cohort.</p><p><strong>Conclusions: </strong>This retrospective study suggests that pN1 OSCC patients with T3-4a stage disease and a low LNY (LNY < 20, levels I-III) may represent a subgroup that derives benefit from adjuvant RT. These exploratory findings suggest a practical model that warrants prospective validation to guide personalized therapy.</p><p><strong>Clinical relevance: </strong>This study provides clinical evidence to help refine the postoperative management of pN1 OSCC patients without high-risk features. It provides a practical stratification tool to guide personalized treatment decisions, aiming to optimize survival outcomes while reducing unnecessary radiotherapy.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does endodontic treatment modify serum inflammatory markers of cardiovascular risk in individuals with asymptomatic apical periodontitis? a systematic review and meta-analysis.","authors":"Randerson Silva Araújo, Erika Bárbara Abreu Fonseca Thomaz, Elma Izze da Silva Magalhães, Juliana Balbinot Hilgert, Soraia de Fátima Carvalho Souza","doi":"10.1007/s00784-026-06857-0","DOIUrl":"10.1007/s00784-026-06857-0","url":null,"abstract":"<p><strong>Objective: </strong>Plasma inflammatory biomarkers linked to cardiovascular risk have been associated with asymptomatic apical periodontitis. However, it remains unclear whether endodontic treatment can reverse these alterations. This review evaluated the effect of endodontic treatment on inflammatory markers in individuals with asymptomatic apical periodontitis.</p><p><strong>Materials and methods: </strong>A comprehensive search was conducted in PubMed/Medline, Embase, Web of Science, Scopus, VHL, gray literature, and reference lists between October and November 2022, with an update in September 2025. Risk of bias was assessed using the Newcastle-Ottawa Scale, and the certainty of evidence using the GRADE approach. Random-effects meta-analysis estimated pooled mean differences (MD) and 95% confidence intervals (95%CI) for serum inflammatory markers concentrations between treated individuals and controls (α = 5%).</p><p><strong>Results: </strong>The search identified 6,295 records; sixteen studies were assessed and eight included in the quantitative synthesis. All studies showed moderate risk of bias, and evidence certainty was very low. Meta-analysis suggested possible reductions in C-reactive protein (CRP) [MD = 0.76 (95% CI: - 0.15, 1.67)] , interleukin-6 (IL-6) [MD = 0.81 (95% CI:-0.27, 1.90)], and tumor necrosis factor-alpha (TNF-α) [MD = 1.04 (95% CI:-0.38, 2.46)] after endodontic treatment, with levels similar to control groups.</p><p><strong>Conclusion: </strong>Evidence, although limited, suggests endodontic treatment may lower serum CRP, IL-6, and TNF-α levels in asymptomatic apical periodontitis patients.</p><p><strong>Clinical relevance: </strong>Endodontic treatment of asymptomatic apical periodontitis may help reduce systemic inflammatory biomarkers associated with cardiovascular risk, reinforcing its potential role beyond local infection control.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalized BMP-2-loaded microspheres combined with a PCL biomimetic scaffold for guiding root development in immature permanent teeth: fabrication and in vitro assessment.","authors":"Zhenyu Yang, Mengyao Yang, Jiaxue Ye, Xiaoyue Lei, Xiaodan Huo, Zirui Qin, Linlong Qi, Yiduo Chen, Yuan Wang, Shuli Deng","doi":"10.1007/s00784-026-06895-8","DOIUrl":"https://doi.org/10.1007/s00784-026-06895-8","url":null,"abstract":"<p><strong>Objectives: </strong>Pulp necrosis in immature permanent teeth arrests root development and compromises long-term prognosis. This study aimed to develop a multifunctional scaffold integrating structural biomimicry, mechanical matching, and sustained growth factor release for orderly root regeneration.</p><p><strong>Materials and methods: </strong>A poly(ε-caprolactone) (PCL) conical scaffold was fabricated via melt electrowriting (MEW) combined with mechanical winding. Bone morphogenetic protein‑2 (BMP‑2)-loaded microspheres were prepared and physically incorporated into the scaffold. The scaffold surface was modified with collagen. Human dental pulp stem cells (hDPSCs) were cultured on the scaffold to evaluate proliferation, adhesion, and osteogenic differentiation.</p><p><strong>Results: </strong>The scaffold exhibited a trilayer \"collagen-microsphere-PCL\" architecture with mechanical compatibility (elastic modulus: 22.5 MPa; fracture strength: 5.29 MPa; elongation: 441.59%). Microspheres (2.86 ± 0.45 μm) showed a gradient distribution and sustained release (70-75% over 90 days). In vitro, the scaffold promoted hDPSC adhesion and proliferation and significantly enhanced osteogenic differentiation with elevated alkaline phosphatase activity, upregulated the expression of osteogenic-related genes, and increased protein levels.</p><p><strong>Conclusions: </strong>The scaffold integrates structural support, controlled growth factor delivery, and a bioactive interface, offering a promising strategy for root development in immature permanent teeth.</p><p><strong>Clinical relevance: </strong>By enabling physiological root development, the scaffold addresses a critical unmet need, offering a viable alternative to conventional root canal therapy.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}