Clinical Oral Investigations最新文献

{"title":"Effect of disinfection and tissue repair with chloramphenicol-tetracycline-ZOE paste on pulp therapy of primary teeth: a systematic review.","authors":"José Antonio Santos Souza, Renata de Oliveira Alves, Priscila Toninatto Alves de Toledo, Heloisa Caroline Mota, Tamires Passadori Martins, Marcelle Danelon, Cristiane Duque, Gabriel Pereira Nunes","doi":"10.1007/s00784-025-06603-y","DOIUrl":"https://doi.org/10.1007/s00784-025-06603-y","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review evaluated the effectiveness of the disinfection and tissue repair technique using chloramphenicol-tetracycline-zinc oxide eugenol paste (CTZ) in pulp therapy of primary teeth.</p><p><strong>Methods: </strong>Following PRISMA 2020 guidelines and PROSPERO registration, comprehensive searches were conducted in multiple databases. Eligibility criteria included clinical studies evaluating CTZ paste in children with pulp necrosis in primary teeth. Risk of bias was assessed using the Cochrane tool for randomized trials and the Newcastle-Ottawa Scale for non-randomized studies. Certainty of evidence was assessed using GRADE.</p><p><strong>Results: </strong>Ten studies, including 496 children aged 3-8 years, met the inclusion criteria. CTZ paste showed high clinical success rates (~ 90-100%) and radiographic success ranging from 72% to 100%. Compared to alternative treatments such as zinc oxide eugenol, 3Mix-MP, formocresol, Guedes-Pinto paste, and pulpectomy, CTZ showed similar outcomes in most comparisons. Additional advantages included reduced postoperative pain, simpler application, and lower procedural costs, suggesting its practicality in pediatric endodontics. Overall, studies presented a low risk of bias. Certainty of evidence was rated moderate for randomized trials and low for non-randomized studies.</p><p><strong>Conclusion: </strong>CTZ paste demonstrates potential as an effective and cost-efficient therapeutic option for the treatment of necrotic primary teeth, showing high clinical and radiographic success rates. Further well-designed, long-term randomized controlled trials are needed to confirm these findings.</p><p><strong>Clinical relevance: </strong>CTZ paste offers simplified, cost-effectiveness, and high success rates make it valuable in pediatric dentistry, especially for uncooperative children and in low-resource settings where conventional endodontic techniques may be unfeasible.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"488"},"PeriodicalIF":3.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qualitative and quantitative analysis of aerosol and droplet dispersion during simulated dental implant procedures using three types of instruments.","authors":"Hiromitsu Morishima, Tomonari Kajita, Jun Watanabe, Kenji Kikuchi, Yoko Iwamatsu-Kobayashi, Wataru Yashiro, Hiroyasu Kanetaka, Hiroshi Egusa, Kensuke Yamauchi","doi":"10.1007/s00784-025-06583-z","DOIUrl":"https://doi.org/10.1007/s00784-025-06583-z","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the generation and dispersion dynamics of aerosols and droplets produced during dental procedures, including implant surgery.</p><p><strong>Materials & methods: </strong>Dental procedures were simulated on a test model using three different instruments: an air turbine handpiece, an ultrasonic device, and an implant motor. Particle behavior was visualized using two types of illumination light sources combined with a high-speed digital camera, enabling both qualitative and quantitative assessments of aerosol and droplet dispersion. Additionally, droplet deposition on water-sensitive paper placed in three different locations was analyzed to compare dispersion patterns among the three instruments.</p><p><strong>Results: </strong>The air turbine handpiece produced the highest luminance intensity (mean ± SD: 112.3 ± 6.4 a.u., n = 9), which was significantly greater than that of the implant motor (78.5 ± 5.2 a.u., n = 9; p < 0.05). For all devices, droplet diffusion was lower during molar treatment than during anterior tooth procedures. Water-sensitive paper analysis revealed increased droplet deposition at the extraoral vacuum site when the vacuum was activated (air turbine: 62 droplets; ultrasonic device: 49 droplets; n = 3 trials each), whereas droplet counts decreased at the patient's forehead.</p><p><strong>Conclusions: </strong>Simulated implant surgery generated less droplet dispersion compared with other dental procedures. Furthermore, the use of an extraoral vacuum markedly reduced droplet spread during various dental treatments.</p><p><strong>Clinical relevance: </strong>These data support layered controls-judicious instrument selection and extraoral suction-to reduce exposure during aerosol-generating procedures. Findings derive from a standardized simulation and should be validated in clinical settings.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"489"},"PeriodicalIF":3.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers during early healing of extraction procedures: a prospective case series.","authors":"Alessandra Toti, Ada Kura, Elena Sticchi, Marco Duvina, Vanni Balestri, Franco Amunni, Paolo Tonelli, Lorenzo Di Cesare Mannelli, Carla Ghelardini, Ester Parisi","doi":"10.1007/s00784-025-06534-8","DOIUrl":"https://doi.org/10.1007/s00784-025-06534-8","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to: (1) evaluate the temporal profiles of VEGF-A, IL-1β, IL-6, IL-10, G-CSF, TNF-α, and β-NGF in surgical wound exudate (WF), comparing them to each patient's baseline values from gingival crevicular fluid (GCF); (2) describe a non-invasive, reliable method for collecting and processing WF to identify molecular markers involved in wound healing.</p><p><strong>Materials and methods: </strong>Twenty-four patients underwent surgical tooth extraction. GCF samples were collected preoperatively (T0), and WF samples 24 h (T1) and 7 days (T2) postoperatively. Protein concentrations were measured using multiplex ELISA and expressed as mean ± SEM.</p><p><strong>Results: </strong>IL-1β significantly increased from 23.58 ± 4.52 (T0) to 83.95 ± 21.39 (T1; p < 0.001). TNF-α rose from 1.82 ± 0.48 to 40.88 ± 11.27 (p < 0.0001), IL-6 peaked at 214.76 ± 38.21 (from 2.37 ± 0.98; p < 0.0001), and VEGF-A increased to 18.50 ± 4.85 (from 3.46 ± 2.19; p < 0.001).</p><p><strong>Conclusions: </strong>The method enabled efficient, non-invasive assessment of protein levels relative to individual baselines, confirming expected biomarker trends in early wound healing.</p><p><strong>Clinical relevance: </strong>Non-invasive biomarker analysis of wound exudate offers a practical approach to monitor healing and support targeted pain reduction and tissue repair strategies.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"490"},"PeriodicalIF":3.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional longitudinal assessment of nasal development with and without Nasoalveolar molding therapy in cleft lip and palate patients.","authors":"Lucas M Ritschl, Klaudia Narbekovas, Denys J Loeffelbein, Alex Grabenhorst, Nils Krautkremer, Hannes Singer, Andrea Grandoch, Helena Kram, Klaus-Dietrich Wolff, Florian D Grill","doi":"10.1007/s00784-025-06589-7","DOIUrl":"10.1007/s00784-025-06589-7","url":null,"abstract":"<p><strong>Background: </strong>Grayson-type presurgical nasoalveolar molding (PNAM) is an established method in presurgical infant orthopedics. This study compared PNAM therapy with conventional palatal plates across cleft types, focusing on nasal morphology.</p><p><strong>Methods: </strong>Seventy-two patients with non-syndromic unilateral (UCLP) and bilateral cleft lip and palate (BCLP) were followed from birth to two years. Three-dimensional (3D) models of the perinasal area were obtained via cast-based scans or 3D photographs; right-sided UCLP cases were mirrored to the left. Two independent reviewers analyzed anatomical landmarks with excellent inter-rater reliability (ICC = 0.88). Initial and two-year measurements were compared between PNAM (NAM group) and conventional treatment (non-NAM group).</p><p><strong>Results: </strong>Of the 72 patients, 46 had UCLP and 26 BCLP. In BCLP, NAM therapy promoted greater columella elongation from 2.1 mm at T₀ to 5.2 mm at T₃ compared to a change from 2.4 mm to 3.5 mm in the non-NAM group (p = 0.300). In UCLP, NAM was also associated with increases in columella (p = 0.387) and improved columella deviation angles, from a median of 48.1° at T₀ to 85.6° at T₃, while non-NAM showed a modest increase from 70.6° to 79.1°, (p = 0.167) Significant reductions were observed cleft-side nostril length (p = 0.027) and width (p = 0.029).</p><p><strong>Conclusion: </strong>This study, using 3D imaging, demonstrates the clinically relevant capability of PNAM to improve nasal morphology compared to conventional treatment, potentially enhancing primary cheiloplasty outcomes. The longitudinal design three-dimensionally tracks preoperative and postoperative changes over two years, with further investigation needed for long-term stability.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"487"},"PeriodicalIF":3.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AIM2 and IFI16 as nucleic acid sensors in Periodontitis-Driven systemic inflammation: emerging evidence and therapeutic implications.","authors":"Shan Wang, Bai Jie","doi":"10.1007/s00784-025-06369-3","DOIUrl":"https://doi.org/10.1007/s00784-025-06369-3","url":null,"abstract":"<p><strong>Objectives: </strong>This review aims to investigate the roles of Absent in Melanoma 2 (AIM2) and Interferon Gamma Inducible Protein 16 (IFI16), as innate immune sensors for cytosolic double-stranded DNA (dsDNA), in the pathogenesis of periodontal disease. Furthermore, this article discusses the significance of these proteins in the interplay between periodontal disease and systemic health conditions.</p><p><strong>Data sources: </strong>This review comprehensively analyzes the involvement of AIM2 and IFI16 in the pathogenesis of periodontal disease, particularly their roles in inflammasome formation and the promotion of IL-1 secretion, through a review of relevant literature. A literature search was conducted using PubMed, Scopus, and Google Scholar databases. Search terms included \"AIM2,\" \"IFI16,\" \"periodontitis,\" \"inflammasome,\" and \"systemic inflammation.\" Only English-language original research articles and reviews were included.</p><p><strong>Study selection: </strong>Studies related to AIM2 and IFI16, especially those examining their roles in periodontitis and systemic diseases, were selected for analysis.</p><p><strong>Conclusions: </strong>Evidence indicates that several inflammasomes, including AIM2 and IFI16, are involved in the pathogenesis of periodontal disease. Polymorphisms in AIM2 and IFI16 genes are linked to an increased severity of periodontal disease. These inflammasomes respond to pathogen or tissue \"danger\" signals by creating multiprotein complexes that cleave the proinflammatory mediator IL-1 into its active form. The study highlights the pivotal role of AIM2 and IFI16 in periodontitis and systemic diseases, particularly in how they shift from homeostasis to disease states in both periodontitis and systemic conditions.</p><p><strong>Clinical significance: </strong>Given that periodontitis is an inflammatory dysbiosis illness affecting overall health, research into inflammasomes like AIM2 and IFI16 is not only significant for understanding the pathogenesis of periodontal disease but also holds clinical relevance in preventing and treating related systemic conditions.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"486"},"PeriodicalIF":3.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of murine fibroblasts or human keratinocytes to micro- and nano-scale titanium particles: the permeability of particles across keratinocytes' monolayers.","authors":"Catarina Pacheco, Redouane Messous, Rui P Moura, Andreia Almeida, Patrícia Silva, Bruno Sarmento, Hassan Bousbaa, Júlio C M Souza","doi":"10.1007/s00784-025-06492-1","DOIUrl":"10.1007/s00784-025-06492-1","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to evaluate the cytocompatibility of micro- and nano-scale commercially pure Titanium (cpTi) particles in contact with fibroblasts and keratinocytes and the penetration of cpTi particles across keratinocytes' layers.</p><p><strong>Method: </strong>Commercially pure titanium (cp-Ti) particles with 50-nm or 1-µm size were chemically and morphologically characterized using a Field Emission Guns Electron Microscopy (FEGSEM), Scanning Transmission Electron Microscope (STEM), and Energy Dispersion Spectrometry (EDAX). Then, the cytotoxic profile of the particles was monitored in contact with murine L929 fibroblasts and TR146 keratinocytes for 1, 4, and 7 days. Further permeability assays were performed across a TR146 monolayer via Transwell^TM model.</p><p><strong>Results: </strong>Physicochemical characterization of cpTi nano-scale particles (cpTi NPs) revealed a mean size at 70 nm and a specific surface area at around ~ 17.2 m²/g, while micro-scale particles (cpTi MP) size ranged from 0.3 up to 5.3 μm with a mean size of 1.4 μm at dry conditions. The optimized de-agglomeration of nanoparticles resulted in an increased specific surface area up to 57.3 m²/g. The metabolic activity of fibroblasts decreased against 50 or 100 µg/ml cpTi over 3 days cell culture while keratinocytes were not affected. Moreover, cpTi NP were internalized and steadily translocated into keratinocyte monolayers, showing an apparent permeability coefficient of 6.65 × 10^-6 cm/s for 50 µg/mL and 3.96 × 10^-6 cm/s for 100 µg/mL.</p><p><strong>Conclusions: </strong>Altogether, nano-scale titanium particles decreased the viability of fibroblasts although a significant viability of keratinocytes has been detected by standard cell culture assays. However, nano-scale titanium particles were found into keratinocytes and even trespassed the cells' layers that could reach other cells and blood vessels in an in vivo scenario. Thus, toxicity of titanium particles depends on their particle size, exposure time, content, and interaction with the surrounding media.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"485"},"PeriodicalIF":3.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticaries effects of the experimental solutions containing niobium pentafluoride.","authors":"Karla Lorene de França Leite, Ana Beatriz Chevitarese, Kenderson Santos Silva, Andréa Vaz Braga Pintor, Livia Rodrigues de Menezes, Lucianne Cople Maia","doi":"10.1007/s00784-025-06561-5","DOIUrl":"https://doi.org/10.1007/s00784-025-06561-5","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To develop, characterize and assess the in vitro effects of experimental niobium pentafluoride (NbF&lt;sub&gt;5&lt;/sub&gt;) solutions on preventing dental caries.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;NbF&lt;sub&gt;5&lt;/sub&gt; solutions were developed and evaluated for fluoride concentration (after 1 and 6 months of preparation) and cytotoxicity (MTT for fibroblasts and preodontoblastic cells). Bovine enamel blocks (n = 176) were randomly assigned into groups of treatment: NbF&lt;sub&gt;5&lt;/sub&gt; (0.25%, 0.5%, and 1% corresponding to 1,263.75; 2,527.5; and 5,055 ppm F⁻), NaF (0.29%, 0.57%, and 1.13% with fluoride levels matched to NbF&lt;sub&gt;5&lt;/sub&gt;), and controls (growth and sterility). The enamel blocks were partially covered and exposed to artificial saliva (1 h) before they were subjected to cariogenic challenge with a multispecies biofilm (37 °C; 24 h and 48 h) in culture medium enriched with 2% sucrose. The pH and total soluble fluoride (TSF) content of the medium were evaluated, and the enamel surface microhardness loss (%SML), volumetric roughness (Sa), tooth structure loss (TSL), and scanning electron microscopy (SEM) results were also evaluated. The data were analyzed by the Kruskal‒Wallis, Mann‒Whitney and Wilcoxon tests (p &lt; 0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The fluoride concentrations remained stable across all storage periods with no significant variation, and similar stabilities were observed for both the NbF&lt;sub&gt;5&lt;/sub&gt; and NaF solutions. The viability of MTT fibroblasts ranged from 94% ± 3.2% for 0.25% NbF&lt;sub&gt;5&lt;/sub&gt; to 76% ± 2.9% for 1.0% NbF&lt;sub&gt;5&lt;/sub&gt;, whereas for MTT preodontoblastic cells, it ranged from 89% ± 3.8-70% ± 4.1% for the same concentrations. In contrast, NaF maintained 100% viability for both cell types, indicating lower cytotoxicity than NbF&lt;sub&gt;5&lt;/sub&gt;. The 1% NbF&lt;sub&gt;5&lt;/sub&gt; and 1.13% NaF solutions presented the highest pH values, with no significant difference between them (p &gt; 0.05). Both compounds also resulted in comparable TSF release patterns, with higher concentrations resulting in lower TSF levels, suggesting greater fluoride uptake into the enamel. The 1% NbF&lt;sub&gt;5&lt;/sub&gt; (21.15 ± 10.12 and 37.31 ± 10.15) and 1.13% NaF solutions (22.12 ± 11.11 and 39.09 ± 13.24) presented the lowest %SMLs at 24 h and 48 h, respectively, with no significant difference between them (p &gt; 0.05). Compared with those of the lower concentration groups, the Sa and TSL of the 1% NbF&lt;sub&gt;5&lt;/sub&gt; and 1.13% NaF groups also exhibited equivalent and superior preventive effects (p &lt; 0.05). SEM and profilometry analysis confirmed similar enamel surface preservation in both the 1% NbF&lt;sub&gt;5&lt;/sub&gt; and the 1.13% NaF groups, with less porosity and mineral loss than in the other groups.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The in vitro results of the 1% NbF&lt;sub&gt;5&lt;/sub&gt; solution revealed the potential of niobium pentafluoride to prevent dental caries, demonstrating equivalent effectiveness to NaF in maintaining enamel integrity,","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"484"},"PeriodicalIF":3.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of combined use of reinforcement materials on the fracture resistance of MOD cavity restorations in endodontically treated teeth.","authors":"Lena Bal, Cangül Keskin, Aybüke Karaca Sakallı, Bilge Özcan, İsen Güleç Koçyiğit","doi":"10.1007/s00784-025-06560-6","DOIUrl":"https://doi.org/10.1007/s00784-025-06560-6","url":null,"abstract":"<p><strong>Objective: </strong>Bucco-lingual/palatinal vertical fractures are among the most common fractures found in mesio-occluso-distal (MOD) cavity restorations of endodontically treated teeth. This study aimed to compare the effects of different new-generation reinforcement materials applied to two cervical and coronal segments of the teeth on the fracture resistance of endodontically treated teeth in their coronal restorations.</p><p><strong>Materials and methods: </strong>A total of 84 freshly extracted, intact human mandibular molars were selected and treated endodontically according to standardized protocols. Standard MOD cavities were prepared, and the specimens were then randomly divided into seven groups (n = 12) based on the combinations of reinforcement materials applied to the cervical or coronal segments, and a control group. The control group had intact teeth. The experimental groups included combinations of flowable composite (Flow), posterior composite (PC), EverX flow (Xflow), EverX posterior (XP), PC, and Ribbond (RIB). After thermocycling, fracture resistance was evaluated using a universal testing machine, and failure patterns were examined under a stereomicroscope. Data were analyzed using the Kruskal-Wallis H test, following a Shapiro-Wilk test that indicated the data did not follow a normal distribution (p < .05).</p><p><strong>Results: </strong>A statistically significant difference was observed between the groups (p < .05). Group 5, which had RIB flow and XP restoration structure, exhibited the highest fracture resistance (1827.90 N), while Group 1, which had Flow and PC restoration structure, demonstrated the lowest fracture resistance (1121.98 N). Fiber-reinforced structures demonstrate superior fracture resistance compared to the other groups (p < .05). There was no significant difference between conventional restoration and Ribbond in the coronal segment (p > .05).</p><p><strong>Conclusion: </strong>Using fiber-reinforced restoration in the cervical segment increases fracture resistance more than in the coronal segment. Fiber-reinforced composites are easily packable and demonstrate higher fracture resistance than Ribbond.</p><p><strong>Clinical relevance: </strong>Resin-reinforced composite combinations used in cervical and coronal applications improve fracture resistance and can be safely applied for MOD cavity restoration of endodontically treated teeth.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"481"},"PeriodicalIF":3.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MALAT1/miR-181b axis mediates the anti-bacterial and anti-inflammatory effects of Galla chinensis in the progression of dental caries : Short title: mechanism of Galla chinensis in dental caries.","authors":"Yao Liu, Qian Min, Hongyan Lu, Feng Yi, Ke Li","doi":"10.1007/s00784-025-06535-7","DOIUrl":"10.1007/s00784-025-06535-7","url":null,"abstract":"<p><strong>Background: </strong>Dental caries is a common oral disease that seriously affects the oral health and quality of life of patients. The aim of this study is to investigate the anti-bacterial and anti-inflammatory effects of Galla chinensis (GC) in dental caries, and explore the underlying mechanisms based on non-coding RNAs.</p><p><strong>Methods: </strong>Mouse and cell models were established by bacterial infection method and LPS induction. The growth of Streptococcus mutans (S. mutans) was detected by growth curves and acid production experiments. ELISA was used to evaluated inflammatory responses, and the effect of GC on the viability of human dental pulp stem cells (HDPSCs) was analyzed by CCK-8 assay. The regulation of MALAT1 and miR-181b expression by GC was detected by qRT-PCR, and the role of the MALAT1/miR-181b axis in the function of GC was examined using rescue experiments by assessing cell and bacterial viability, inflammation and S. mutans infection.</p><p><strong>Results: </strong>GC significantly inhibited the growth and acid production of S. mutans, the levels of inflammatory factors and MALAT1 levels, and promoted miR-181b expression in both cell and animal models. Additionally, the proliferation of disease cell model was elevated by GC. miR-181b as a target of MALAT1, was inhibited by MALAT1 in cell model, and the MALAT1/miR-181b axis was found to mediate the regulatory effects of GC on S. mutans growth, infection-induced inflammatory, HDPSC viability and inflammation.</p><p><strong>Conclusions: </strong>GC inhibits S. mutans growth and inflammatory responses in cells and animal models through the MALAT1/miR-181b axis. This study highlights the potential clinical value of targeting the MALAT1/miR-181b axis with GC, which may offer a novel therapeutic strategy for the prevention and treatment of dental caries in clinical practice.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"483"},"PeriodicalIF":3.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12479602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofilm-resistant zwitterionic resin-based adhesive for orthodontic bracket-tooth interfaces: an in vitro evaluation.","authors":"Min-Ji Kim, EunJu Kim, Utkarsh Mangal, Ji-Young Seo, Jung-Yul Cha, Kee-Joon Lee, Yoon Jeong Choi, Jae-Sung Kwon, Hyung-Seog Yu, Sung-Hwan Choi","doi":"10.1007/s00784-025-06572-2","DOIUrl":"https://doi.org/10.1007/s00784-025-06572-2","url":null,"abstract":"<p><strong>Objectives: </strong>An in vitro study to verify the potential effectiveness of an orthodontic adhesive incorporating a polybetaine zwitterionic mixture in preventing biofilm formation.</p><p><strong>Materials and methods: </strong>2-methacryloyloxyethyl phosphorylcholine (MPC) and sulfobetaine methacrylate (SBMA), were added to An adhesive at 1 wt% (MS1) And 3 wt% (MS3). The MS0 group had no zwitterions. Flowability measurement, shear bond strength (SBS), the adhesive remnant index (ARI), and the contact angle were assessed. Bracket bonding was performed, and cross-sections were examined using scanning electron microscopy (SEM). Biofilm formation was analyzed using confocal laser scanning microscopy. Statistical analyses were conducted using R software. Wilcoxon tests with the Holm correction were used for non-parametric data, and pairwise t-tests with the Bonferroni correction were used for parametric data. Significance was set at P < 0.0001.</p><p><strong>Results: </strong>Flow analysis showed no significant differences in the experimental groups compared to the MS0 group (P > 0.05). The SEM images confirmed uniform bonding in all groups. SBS decreased significantly with higher MS content (P < 0.0001), And ARI scores shifted with the addition of zwitterionic mixtures, increasing scores to 1 And 2. MS3 showed a significantly lower contact angle compared to MS0 (P < 0.05). MS3 exhibited reduced biofilm formation and lower fluorescence intensity (P < 0.05).</p><p><strong>Conclusions: </strong>Despite reductions in SBS, all adhesives remained at minimum acceptable levels. The 3 wt% zwitterionic adhesive effectively suppressed biofilm formation and may help prevent demineralization.</p><p><strong>Clinical relevance: </strong>An orthodontic adhesive containing a zwitterionic mixture can satisfy clinical properties and prevent side effects due to biofilm formation.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"478"},"PeriodicalIF":3.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}