Clinical Oral Investigations最新文献

{"title":"Safety and preliminary efficacy of an electrically stimulated implant for mandibular bone regeneration: a pilot study in a large animal model.","authors":"Peer W Kämmerer, Nadja Engel, Rainer Bader, Vivien Engel, Bernhard Frerich, Diana Heimes, Justin Kröger, Laura Lembcke, Franz Plocksties, Hendrikje Raben, Ursula van Rienen, Armin Springer, Dirk Timmermann, Julius Zimmermann, Michael Dau","doi":"10.1007/s00784-025-06303-7","DOIUrl":"10.1007/s00784-025-06303-7","url":null,"abstract":"<p><strong>Objectives: </strong>Large mandibular defects present challenges for bone regeneration. This pilot study evaluates the safety and preliminary efficacy of direct electrical stimulation (ES) on tissue healing in a preclinical model, testing whether ES can enhance bone formation in critical-size mandibular defects.</p><p><strong>Materials and methods: </strong>Six adult mini pigs with critical-size mandibular defects were used in a split-mouth design. The test group (n = 6) received 0.5 V AC/20 Hz ES for 3 × 45 min daily over three weeks, while the control group (n = 6) had no stimulation. Safety, early bone growth, and soft tissue effects were assessed at three locations: S1 (cancellous bone interface), S2 (middle of the defect), and S3 (pristine dense bone).</p><p><strong>Results: </strong>The ES group showed no adverse effects, confirming implant safety. The ES group exhibited significantly higher bone formation, particularly in S2 and S3. Enhanced vascularization and immune response, in terms of increased mast cells, were also observed in S2.</p><p><strong>Conclusions: </strong>The implant device with ES is safe and promotes bone formation and vascularization in select sub-regions (S2 and S3). However, ES alone may not suffice for complete bone regeneration in critical-sized defects, and further optimization is needed.</p><p><strong>Clinical relevance: </strong>This study demonstrates the potential of ES to improve bone healing in large mandibular defects, offering insights for clinical use in maxillofacial reconstruction.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 5","pages":"226"},"PeriodicalIF":3.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obsessive-compulsive symptoms and traits in patients with burning mouth syndrome: a cross-sectional multicentric analysis.","authors":"Luca Pellegrini, Federica Canfora, Giulia Ottaviani, Cristina D'Antonio, Katia Rupel, Michele Davide Mignogna, Matteo Biasotto, Amerigo Giudice, Gennaro Musella, Vito Carlo Alberto Caponio, Gianrico Spagnuolo, Carlo Rengo, Giuseppe Pecoraro, Massimo Aria, Luca D'Aniello, Umberto Albert, Daniela Adamo","doi":"10.1007/s00784-025-06293-6","DOIUrl":"10.1007/s00784-025-06293-6","url":null,"abstract":"<p><strong>Objective: </strong>This study investigates the frequency and characteristics of obsessive-compulsive (OC) symptoms and Obsessive-Compulsive Personality Disorder (OCPD) in patients with Burning Mouth Syndrome (BMS).</p><p><strong>Background: </strong>Obsessive-Compulsive Disorder (OCD) is a chronic condition involving intrusive thoughts (obsessions) and repetitive behaviors (compulsions), while Obsessive-Compulsive Personality Disorder (OCPD) is a personality disorder characterized by specific traits such as perfectionism, rigidity and need for control. Both conditions frequently overlap, but their prevalence in patients with BMS has never been explored.</p><p><strong>Materials and methods: </strong>A total of 151 BMS patients were assessed using the Obsessive-Compulsive Inventory-Revised (OCI-R), Compulsive Personality Assessment Scale (CPAS), Visual Analog Scale (VAS), Short-Form McGill Pain Questionnaire (SF-MPQ), Hamilton Anxiety and Depression scales (HAM-A, HAM-D), Pittsburgh Sleep Quality Index (PSQI), and Epworth Sleepiness Scale (ESS). Patients were grouped based on OCI and CPAS scores.</p><p><strong>Results: </strong>n = 123 (81.6%) of our sample were females, with a mean age of 63.19 ± 12.2 years. Clinically significant OC symptoms (OCI-R > 21) were observed in 41.7% of the sample, while 37% met OCPD criteria; both OC symptoms and OCPD were present in 24.5% of patients.</p><p><strong>Conclusions: </strong>BMS patients show a high prevalence of OC symptoms and OCPD traits, which should be taken into account by clinicians and considered in the therapeutic approach, given that they could complicate symptom management.</p><p><strong>Clinical relevance: </strong>By identifying these symptoms and traits through OCI-R and CPAS, clinicians may improve treatment strategies, in the perspective of a multidisciplinary tailored and personalized approach.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"223"},"PeriodicalIF":3.1,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different remineralization agents on shear bond strength of orthodontic brackets: in vitro study.","authors":"Gulsevim Oda, Ozge Muftuoglu Guler","doi":"10.1007/s00784-025-06312-6","DOIUrl":"10.1007/s00784-025-06312-6","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate the effect of different remineralization agents-Proshield Fluoride Varnish (FV), GC MI Varnish (MI), and ROCS Medical Mineral Gel (ROCS MMG)-on the shear bond strength (SBS) of orthodontic brackets after application at various time intervals.</p><p><strong>Methods: </strong>A total of 120 human premolar teeth were divided into 10 groups (n = 12). The experimental groups received different remineralization agents (FV, MI, ROCS MMG) at time intervals of 1, 15, and 30 days, while the control group received no application. The enamel surfaces were etched, and metal orthodontic brackets were bonded using a light-cured composite adhesive. SBS was measured using a universal testing machine, and adhesive remnant index (ARI) scores were assessed after bracket debonding.</p><p><strong>Results: </strong>No statistically significant differences were observed in SBS among all experimental groups and the control group (P = 0.307). SBS values for the FV, MI, and ROCS MMG groups did not differ significantly at the 1, 15, or 30-day intervals. ARI scores also showed no significant differences between groups (P = 0.370).</p><p><strong>Conclusion: </strong>The application of Proshield Fluoride Varnish, GC MI Varnish, and ROCS Medical Mineral Gel at different time intervals did not affect the shear bond strength of orthodontic brackets. These remineralization agents can be safely used to prevent white spot lesions and caries before orthodontic treatment at time intervals of 1, 15, and 30 days without compromising bond strength.</p><p><strong>Clinical relevance: </strong>Proshield FV, GC MI Varnish, and ROCS MMG safely prevent white spot lesions and caries without affecting bracket bond strength.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"224"},"PeriodicalIF":3.1,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presumed periodontitis and multimorbidity patterns: a prospective cohort study in the UK Biobank.","authors":"Xuezhen Chen, Xiaoqing You, Chunting Chen, Yongsheng Yang, Haomin Yang, Fei He","doi":"10.1007/s00784-025-06309-1","DOIUrl":"https://doi.org/10.1007/s00784-025-06309-1","url":null,"abstract":"<p><strong>Objectives: </strong>To examine the pattern of multimorbidity among people with high risk of periodontitis.</p><p><strong>Materials and methods: </strong>Over 358,000 UK Biobank participants aged 40-69 years at baseline who took part in the baseline assessment and answered mouth/teeth dental problems were included (2006-2010). Cox proportional hazard models and logistic regression models were used to estimate the association of the risk factors of periodontitis with chronic diseases and multimorbidity, stratified by follow-up time.</p><p><strong>Results: </strong>A total of 154,985 participants developed multimorbidity during follow-up. We observed increased risk of multimorbidity among participants with presumed periodontitis (adjusted HR = 1.06, 95% confidence interval [CI] = 1.05-1.08), especially in those participants with age < 50 years old (adjusted HR = 1.11, 95% CI = 1.08-1.14). Among the different multimorbidity patterns, presumed periodontitis was mainly associated with the mental disorder pattern and metabolic and vascular disease pattern.</p><p><strong>Conclusions: </strong>Presumed periodontitis was positively associated with multimorbidity, even more so in younger age. We need to pay more attention to the prevention of periodontitis in the early stage to reduce the burden of multimorbidity in the future.</p><p><strong>Clinical relevance: </strong>Early life interventions to prevent periodontitis are crucial to reduce the incidence of multimorbidity and enhance the quality of life in older adults. Additionally, greater attention should be given to the mental and cardiovascular metabolic health of patients with periodontitis.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"222"},"PeriodicalIF":3.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of cusp reduction and fiber reinforcement on cusp deflection and fracture strength of restored endodontically treated molar teeth.","authors":"Merve Aksoy Yüksek, Cemile Kedici Alp, Ceyda Sarı, Oya Bala","doi":"10.1007/s00784-025-06297-2","DOIUrl":"10.1007/s00784-025-06297-2","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the influence of various cusp reduction amounts, localization, and the use of coronal polyethylene fiber on cusp deflection in endodontically treated molar teeth.</p><p><strong>Material and methods: </strong>The 120 intact molar teeth observed in the study were divided into 12 groups, and standard MOD cavities were prepared after the root canal treatment was completed. Groups 1 and 2 were determined as positive and negative control groups, respectively. Two cusp reductions were applied to Groups 3, 4, 5, and 6, and functional cusps reduction were applied to Groups 7, 8, 9, and 10. The cusp reductions were either 1.5 mm or 3 mm. After preparation, Groups 3, 5, 7, 9, and 11 were restored with Filtek One Bulk-Fill, while Groups 4, 6, 8, 10, and 12 were restored with Filtek One Bulk-Fill + Ribbond. As the teeth were restored, the amount of cusp deflection was measured with a twin channel deflection gauge device. The fracture strength of the teeth was measured using a universal test machine. The experimental results were statistically evaluated with two-way and one-way ANOVA tests.</p><p><strong>Results: </strong>The reinforcement of the coronal structure with fiber reduced cusp deflection and increased fracture strength. The statistically significantly less cusp deflection was obtained in the groups with the same cavity design of having fiber placed than in the groups without fiber placed (p < 0.001). The statistically significantly lower fracture strength was obtained in Group 11 than all the reduced groups except Groups 5 and 7 (p < 0.05). The statistically lower fracture strength was obtained in Group 12, than in Group 4 (p < 0.05). Experimental groups with cusp reduction showed less cusp deflection (p < 0.001) and higher fracture strength (p < 0.05) than experimental groups without cusp reduction.</p><p><strong>Conclusions: </strong>Fiber application and cusp reduction may have positive effects in terms of reducing cusp deflection and increasing fracture strength in direct restoration cases involving excessive substance loss in endodontically treated teeth.</p><p><strong>Clinical relevance: </strong>Incorporating cusp reduction and coronal fiber applications in treatment plans for endodontically treated teeth ensures that restorations are both functional and durable.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"220"},"PeriodicalIF":3.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Whitening Agents (Whitening Rinse and Carbamide Peroxide) on Stained Flowable and Packable Composite Aligner Attachments.","authors":"Ezgi Atik, Ülkü Tuğba Kalyoncuoğlu","doi":"10.1007/s00784-025-06298-1","DOIUrl":"10.1007/s00784-025-06298-1","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;This study aimed to verify the effects of whitening agents on the color stability of aligner attachments made from flowable and packable composite resins after staining with coffee and wine.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Two composite groups were used for attachment preparation on epoxy resin master models: G-ænial Universal Injectable flowable (Group 1, N = 42) and G-ænial Posterior packable (Group 2, n = 42). Initial staining of samples was performed with coffee and red wine respectively, with a total period of 6 days representing 6 months of consumption. After staining, each main group was randomly divided into 3 groups related to distilled water as the control (Distilled Water (DW), n = 14) and whitening agents (Whitening Oral Rinse (WR), n = 14) (Carbamide Peroxide 22% (CP), n = 14) for a 14-day test period. Before staining (T0), after staining (T1), and after whitening (T2), color measurements were made with Vita Easy Shade V. ΔE&lt;sub&gt;00&lt;/sub&gt; formula was used to determine color differences between T0-T1 (ΔE&lt;sub&gt;001&lt;/sub&gt;), T1-T2 (ΔE&lt;sub&gt;002&lt;/sub&gt;) and T0-T2 (ΔE&lt;sub&gt;003&lt;/sub&gt;) color measurement periods. Also, attachments' whiteness change before and after whitening procedures was calculated using the Whiteness Index for Dentistry (WI&lt;sub&gt;D&lt;/sub&gt;) formula considering as ΔWI&lt;sub&gt;D1&lt;/sub&gt; = WI&lt;sub&gt;D&lt;/sub&gt;(T2) - WI&lt;sub&gt;D&lt;/sub&gt;(T0) and ΔWI&lt;sub&gt;D2&lt;/sub&gt; = WI&lt;sub&gt;D&lt;/sub&gt;(T2) - WI&lt;sub&gt;D&lt;/sub&gt;(T1).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;After immersion in staining solutions, ΔE&lt;sub&gt;001&lt;/sub&gt; values of Group 2 were significantly higher than of values of Group 1 (p &lt; 0.001). In Group 1, ΔWI&lt;sub&gt;D2&lt;/sub&gt; values in the WR and CP groups were found to be significantly higher than measurements in the DW group (p = 0.049 and p = 0.001). In Group 2, the value of ΔE&lt;sub&gt;002&lt;/sub&gt; for subgroup CP was significantly higher than that for DW group (p = 0.023). Also, the ΔE&lt;sub&gt;003&lt;/sub&gt; measurement of the WR group was higher than the measurement of the CP group (p &lt; 0.001). In Group 2, the ΔWI&lt;sub&gt;D1&lt;/sub&gt; measurement of the CP group was lower than the measurements of the DW (p &lt; 0.001) and WR (p = 0.014) groups. According to ΔWI&lt;sub&gt;D2&lt;/sub&gt; measurement in Group 2, CP measurements were higher than DW (p &lt; 0.001) and WR (p = 0.024) measurements. Statistically significant differences were determined between the composite types for DW, WR, CP whitening types in terms of ΔE&lt;sub&gt;003&lt;/sub&gt; and ΔWI&lt;sub&gt;D1&lt;/sub&gt; measurements and between the composites for CP whitening type in terms of ΔWI&lt;sub&gt;D2&lt;/sub&gt; measurements (p &lt; 0.05). For all significant differences, measurements for Group 2 were found to be higher than for Group 1.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The color change of packable composite was more pronounced than that of the flowable composite after staining. Between whitening and staining stages, especially in packable composite group, carbamide peroxide whitening agent significantly effected the color and whiteness of the attachments c","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"221"},"PeriodicalIF":3.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of Lycium barbarum in treating periodontitis based on network pharmacology, molecular docking, and experimental validation.","authors":"Lin-Sha Ma, Xue-Ting Jia, Fa-Quan Hu, Yu-Jiao Zheng, Xiao-Feng Huang, Xiaohui Rausch-Fan, Xiao-Chuan Fan","doi":"10.1007/s00784-025-06313-5","DOIUrl":"10.1007/s00784-025-06313-5","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the active components of Lycium barbarum (L. barbarum) and their therapeutic role in periodontitis through network pharmacology, molecular docking, and experimental validation.</p><p><strong>Materials and methods: </strong>The active ingredients and targets of L. barbarum and the targets of periodontitis were retrieved from multiple pharmaceutical databases. An L. barbarum-active ingredients-targets network diagram was constructed by Cytoscape software. A protein interaction network was constructed using the STRING platform. Hub targets were enriched using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Key periodontitis targets were analyzed using molecular docking and molecular dynamics. Raw264.7 cells were stimulated with lipopolysaccharide (LPS) and treated with L. barbarum extract at concentrations of 1, 5, and 10 μg/mL. Anti-inflammatory effects were evaluated by measuring IL-1β, IL-6, and TNF-α levels using enzyme-linked immunosorbent assay. Osteoclast differentiation was induced for 5 d, MMP9, RANK, and TRAP mRNA expression were quantified by real-time polymerase chain reaction (RT-PCR), and osteoclast formation was confirmed via tartrate-resistant acid phosphatase (TRAP) staining.</p><p><strong>Results: </strong>We identified 45 active ingredients and 205 potential targets of L. barbarum alongside 3,520 periodontitis targets. A total of 132 L. barbarum-periodontitis co-targets were identified. The hub genes of L. barbarum regulate periodontitis and have strong binding activity with active ingredients. The involvement of inflammatory factors, such as IL-1β and IL-6, and signaling pathways, including TNF, IL-17, and HIF-1, was verified. L. barbarum extract significantly reduced IL-1β, IL-6, and TNF-α production in LPS-stimulated Raw264.7 cells. Additionally, L. barbarum extract downregulated MMP9, RANK, and TRAP expression and inhibited osteoclast differentiation, as evidenced by the reduced number of TRAP-positive multinucleated cells.</p><p><strong>Conclusions: </strong>The bioactive constituents of L. barbarum were verified to exert anti-inflammatory and osteoclastogenesis-inhibitory effects by targeting inflammatory mediators, oxidative stress, and TNF, IL-17, and HIF-1 signaling pathways, demonstrating potential therapeutic benefits for periodontitis.</p><p><strong>Clinical relevance: </strong>L. barbarum. may serve as a natural therapeutic agent for periodontitis by modulating immune responses and targeting key inflammatory mediators and signaling pathways.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"219"},"PeriodicalIF":3.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Craniofacial integration and modularity in untreated cleft lip and palate.","authors":"Sariesendy Sumardi, Anne Marie Kuijpers-Jagtman, Benny S Latief, Hans L L Wellens, Piotr S Fudalej","doi":"10.1007/s00784-025-06296-3","DOIUrl":"10.1007/s00784-025-06296-3","url":null,"abstract":"<p><strong>Objectives: </strong>To quantify craniofacial variation, integration, and modularity in untreated adults with orofacial clefts who had not undergone surgery, as well as in unaffected controls.</p><p><strong>Materials and methods: </strong>Fourteen cephalometric landmarks depicting the skull base, maxilla, and mandible were identified on lateral cephalograms of 295 adult Proto-Malayid individuals. The sample included 243 individuals with unoperated clefts-179 with complete unilateral cleft lip and alveolus (UCLA, mean age 23.7 years) and 66 with complete unilateral cleft lip, alveolus, and palate (UCLAP, mean age 24.5 years)-and 50 unaffected controls (NORM, mean age 21.2 years). Geometric morphometrics were used to analyze craniofacial shape variability, integration, and modularity. Principal component analysis (PCA) was used to assess shape variability, while canonical variates analysis (CVA) was used to evaluate group differences by calculating Mahalanobis and Procrustes distances. Integration and modularity were tested for five scenarios: (1) skull base vs. maxilla vs. mandible, (2) skull base with maxilla vs. mandible, (3) skull base with mandible vs. maxilla, (4) skull base vs. maxilla with mandible, and (5) anterior vs. posterior modules. The RV coefficient and covariance ratio were used to assess covariation strength.</p><p><strong>Results: </strong>The first 6 principal components (PC1-PC6) explained 72% of the total shape variability, with vertical shape variation and sagittal relationships being the primary sources of variability. Craniofacial shape varied significantly among the groups, with the largest Mahalanobis and Procrustes distances observed between the NORM and UCLAP groups (p < 0.001), and the smallest between the UCLA and UCLAP groups (p < 0.001). Modularity and integration patterns differed between cleft-affected individuals and controls; Those with clefts had anterior and posterior modules separated by the pterygomaxillary plane, while controls showed distinct modules for the skull base, maxilla, and mandible or combined skull base-mandible and maxilla.</p><p><strong>Conclusions: </strong>Unoperated unilateral UCLA and UCLAP affect craniofacial integration and modularity.</p><p><strong>Clinical relevance: </strong>These insights highlight the importance of individualized treatment approaches that consider congenital craniofacial organization, potentially improving long-term functional and aesthetic outcomes.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"218"},"PeriodicalIF":3.1,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11958437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: In-vitro validation of a new method to assess the clinical accuracy of complete arch impressions.","authors":"Moritz Waldecker, Katherina Schessler, Wolfgang Bömicke, Peter Rammelsberg, Stefan Rues","doi":"10.1007/s00784-025-06292-7","DOIUrl":"https://doi.org/10.1007/s00784-025-06292-7","url":null,"abstract":"","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"216"},"PeriodicalIF":3.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The landscape of the microbiome at different stages of root caries.","authors":"Mengzhen Ji, Kaixin Xiong, Di Fu, Yaqi Chi, Ye Wang, Lin Yao, Xueqin Yang, Yujia Yan, Hualing Zhu, Yanyao Li, Biao Ren, Ling Zou","doi":"10.1007/s00784-025-06301-9","DOIUrl":"https://doi.org/10.1007/s00784-025-06301-9","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the relationship between microorganisms and root caries, identify core species, and explore their interactions.</p><p><strong>Materials and methods: </strong>Thirty patients with different levels of root caries were included. Plaques from superficial (n = 30) and deep root caries (n = 30) and sound root surfaces (n = 30) were collected. Microbial diversity and composition across different stages of root caries were analyzed using 16 S rRNA and 18 S rRNA high-throughput sequencing. Wilcoxon paired comparisons were conducted to minimize individual variations. LefSe analysis was performed to identify stage-specific microbial enrichment. In vitro biofilm models of C. albicans, S. mutans and A. viscosus were established to examine the effects of C. albicans on biofilm formation, virulence factor expression, and metabolic pathway regulation. Fungal transcriptomes were sequenced to explore how fungal species affect bacterial growth and cariogenicity.</p><p><strong>Results: </strong>No significant differences in microbial diversity or structure were observed, but relative abundances of some species differed significantly (p < 0.05). LefSe analysis showed that the genus Streptococcus, Actinomyces, Lactobacillus, and Bacillus were enriched in superficial caries, whereas Prevotella was enriched in deep lesions. C. albicans was the predominant fungal species in root plaques and positively correlated with S. mutans and Actinomyces sp. HMT448. C. albicans promoted the growth, biofilm formation, and cariogenicity of S. mutans and A. viscosus via the arginine biosynthesis pathway.</p><p><strong>Conclusion: </strong>Oral microecology is stable, and species imbalance is a key factor in root caries. Cross-kingdom interactions between S. mutans, A. viscosus, and C. albicans enhance cariogenic biofilms via the arginine biosynthesis pathway, offering insights for clinical treatments of root caries.</p><p><strong>Clinical relevance: </strong>Our study revealed the first landscape of the microbiome from different stages of root caries and indicated that targeting the interactions of core species may be a practical way to prevent and treat clinical root caries.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 4","pages":"217"},"PeriodicalIF":3.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}