Clinical Oral Investigations最新文献

{"title":"Influence of surface treatment on roughness, fracture force, flexural strength, and dynamic loading of a 3D-printed crown and bridge material.","authors":"Michael Benno Schmidt, Sebastian Hahnel, Angelika Rauch, Martin Rosentritt","doi":"10.1007/s00784-025-06518-8","DOIUrl":"https://doi.org/10.1007/s00784-025-06518-8","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate how surface treatment affects fracture force, flexural strength, and dynamic loading cycles until failure of 3D-printed restorations.</p><p><strong>Materials and methods: </strong>Specimens (7 groups; n = 8 per group) were 3D-printed from an acrylate-based crown and bridge material. After cleaning and post-polymerization, specimens were treated with either silicon carbide paper (1000 grit; 1000/4000 grit) or blasting (Al₂O₃; 1 bar/125 µm; 2 bar/125 µm; 1 bar/250 µm) to simulate laboratory treatment. Surface roughness (Arithmetic mean Sa/maximum roughness height Sz; ISO 25178-2); fracture force (FF) and biaxial flexural strength (BFS; ISO 6872) were determined. The number of dynamic load cycles (LC) to failure was determined under cyclic loading in a BFS staircase approach.</p><p><strong>Statistics: </strong>ANOVA, Bonferroni-test, Kaplan-Meier survival, Pearson correlation; α = 0.05.</p><p><strong>Results: </strong>BFS ranged between 94.4 MPa and 199.9 MPa, FF between 260.6 N and 428.6 N and Sa/Sz between 0.0/1.0 μm and 1.8/18.4 μm. BFS, FF and Sa/Sz showed significant differences between the treatments (p < 0.001) and individual groups (p ≤ 0.013). Mean LC ranged between 204,364 and 267,637 cycles. ANOVA comparisons (p = 0.706) and Log Rank test (Chi²: 10,835; p = 0.094; Fig. 2) revealed no significant differences between the loading cycles. Surface treatment with either silicon carbide papers or blasting protocols had a significant influence on FF, BFS, Sa, and Sz, but not on LC.</p><p><strong>Conclusions: </strong>Surface treatment affected the fracture force and biaxial fracture strength of a 3D-printed crown. It showed no influence on the long-term dynamic behavior.</p><p><strong>Clinical relevance: </strong>Smooth surfaces improve the stability of a restoration fabricated from 3D-printing resins. Extensive surface roughness treatment before cementation can reduce the stability of a crown.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"426"},"PeriodicalIF":3.1,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Combined catalytic strategies applied to in-office tooth bleaching: whitening efficacy, cytotoxicity, and gene expression of human dental pulp cells in a 3D culture model.","authors":"Rafael Antonio de Oliveira Ribeiro, Victória Peruchi, Igor Paulino Mendes Soares, Filipe Koon Wu Mon, Diana Gabriela Soares, Josimeri Hebling, Carlos Alberto de Souza Costa","doi":"10.1007/s00784-025-06530-y","DOIUrl":"https://doi.org/10.1007/s00784-025-06530-y","url":null,"abstract":"","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"427"},"PeriodicalIF":3.1,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of systemic resveratrol on non-surgical periodontal treatment of smokers: A 12-month randomized clinical trial.","authors":"Vanessa Haguihara Lucchesi, Ana Paula Oliveira Giorgetti, Mônica Grazieli Corrêa, Vanessa G A Pecorari, Bruno Braga Benatti, Howard C Tenenbaum, Fabiano Ribeiro Cirano, Suzana Peres Pimentel, Márcio Zafallon Casati","doi":"10.1007/s00784-025-06517-9","DOIUrl":"https://doi.org/10.1007/s00784-025-06517-9","url":null,"abstract":"<p><strong>Objective: </strong>Smoking patients demonstrate an elevated risk of periodontitis development and respond poorly to periodontal therapy as compared to nonsmokers, and resveratrol (RSV) demonstrated a positive effect in the reduction of periodontitis progression in both animal and clinical trials. However, to the authors' knowledge, no clinical study has assessed the impact of resveratrol under smoking conditions. Thus, this trial aimed to evaluate the effect of systemic administration (SA) of RSV adjunct to full-mouth ultrasonic debridement (FMUD) of periodontitis smoking patients (PSP).</p><p><strong>Materials and methods: </strong>Thirty-eight individuals were randomly assigned to two groups:Placebo ( n = 19) -FMUD and placebo for 180 days; RSV ( n = 19) FMUD and RSV (500 mg/day) for 180 days. Clinical and immunoinflammatory outcomes were assessed at baseline, 3-, 6-, and 12-months post-therapy, and microbiological outcomes were evaluated at baseline, 3-, 6- months post-therapy.</p><p><strong>Results: </strong>RSV appeared to induce lower PD [2.96 (0.41) - 3 months; 2.85 (0.40) - 6 months; 2.80 (0.35)- 12 months], CAL [4.02 (0.90) - 3 months; 4.04 (0.81) - 6 months; 3.87 (0.78) - 12 months], and PMG [2.20 (0.56) - 3 months; 2.28 (1.14) - 6 months; 2.32 (3.27) - 12 months] readings as compared to Placebo [PD: 3.22 (0.51) - 3 months; 3.07 (0.42) - 6 months; 3.02 (0.42) - 12 months; CAL: 4.43 (0.99) - 3 months; 4.24 ((0.89) - 6 months; 4.39 (0.93) - 12 months; PMG: 2.50 (0.50) - 3 months; 2.53 (0.45) - 6 months; 2.67 (0.46) - 12 months] throughout the time (p < 0.05). The concentration of Aggregatibacter actinomycetemcomitans (Aa) was significantly higher in moderate [2.29 (1.10); 1.61 (1.02) for PL and RSV, respectively] and deep PD [2.39 (1.14); 1.73 (0.90) for PL and RSV respectively] at 3 months for the Placebo group (p < 0.05). Additionally, Aa levels were lower at 6 months in the deep sites for the RSV group (p < 0.05) [1.77 (0.94); 2.23 (1.08) for PL and RSV, respectively]. Immunoinflammatory analysis showed lower levels of IL-1β at 3-month periods in deep sites in the RSV group [92.6 ± 84.2; 35.36 (52.92) for PL and RSV, respectively] and lower concentrations of IL-6 in the RSV group at 3 and 12 months in both moderate [8.11 (9.50); 4.67 (4.20) - 3 months for PL and RSV, respectively; 8.01 ± 3.52; 5.33 (4.14) - 12 months for PL and RSV, respectively]; and deep sites [4.69 (3.06); 3.57 (3.73) - 3 months for PL and RSV, respectively]; 3.50 (2.67); 2.10 (0.89) - 12 months for PL and RSV, respectively] (p < 0.05).</p><p><strong>Conclusion: </strong>In conclusion, systemic administration of RSV improves clinical results and modulates IL-1β at 3 months, IL-6 at 3- and 6- months, in deep sites of smoking patients when associated with FMUD.</p><p><strong>Trial registration: </strong>Rebec identifier https//ensaiosclinicos.gov.br/rg/RBR3gt65c.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"428"},"PeriodicalIF":3.1,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of maxillary occlusal splints on mobile posterior teeth in patients with stage III or IV periodontitis during steps 1 and 2 of periodontal therapy: a randomized parallel-controlled clinical trial.","authors":"Siyu Hui, Yilin Hong, Shuangshuang Chen, Yang Bao, Jia Song, Yuan Zhao, Xiaolin Tang","doi":"10.1007/s00784-025-06506-y","DOIUrl":"10.1007/s00784-025-06506-y","url":null,"abstract":"<p><strong>Aim: </strong>The present study aims to assess the efficacy of elastic occlusal splints on mobile posterior teeth in patients with stage III or IV periodontitis during Steps 1 and 2 of periodontal therapy.</p><p><strong>Materials and methods: </strong>Thirty-eight periodontitis patients, each with at least one mobile maxillary posterior tooth (mobility degree II or III), were randomly assigned to either the Splint group (n = 19), which received maxillary elastic occlusal splints, or the Control group (n = 19), without splints treatment. All patients received Steps 1 and 2 of periodontal therapy. Tooth mobility (TM), masticatory efficiency, probing depth (PD), evaluation of subjective comfort and other parameters were assessed at baseline (T0), 2 ~ 4 weeks (T1) and 6 ~ 8 weeks (T2) after the subgingival scaling. Masticatory efficiency was represented by optical density (OD). The subjective comfort score was recorded using a Visual Analogue Scale (VAS).</p><p><strong>Results: </strong>The value of OD in the Splint group was significantly greater than that in the Control group from T0 to T2 (0.19 ± 0.08 versus 0.09 ± 0.09, p < 0.05) and from T1 to T2 (0.11 ± 0.06 versus 0.02 ± 0.06, p < 0.05). Compared with the Control group, patients in the Splint group experienced significantly greater reduction in TM of mobile posterior teeth throughout each treatment period, as well as a more pronounced decrease in PD from T0 to T2. The score of VAS was 8.34 ± 1.10 at T1 and 8.52 ± 0.91 at T2 with elastic occlusal splints.</p><p><strong>Conclusion: </strong>The use of elastic occlusal splints as adjuncts during Steps 1 and 2 of periodontal therapy resulted in additional improvement of the masticatory efficiency of periodontitis patients, as well as the reduction of TM and PD of the mobile posterior teeth.</p><p><strong>Trial registration: </strong>ChiCTR2400090295.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"425"},"PeriodicalIF":3.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of charcoal-based dentifrices on the surface integrity and gloss of lithium disilicate glass-ceramics.","authors":"Victor Martins Stabile, Ana Caroline Lima Colombino, Andrey Gonçalves Emídio, Lourenço Correr-Sobrinho, Roberta Caroline Bruschi Alonso, Regina Maria Puppin-Rontani","doi":"10.1007/s00784-025-06494-z","DOIUrl":"https://doi.org/10.1007/s00784-025-06494-z","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the effects of commercially charcoal-based dentifrices on the surface roughness and gloss retention of glazed lithium disilicate glass-ceramics subjected to simulated toothbrushing.</p><p><strong>Materials and methods: </strong>Seventy-two glazed lithium disilicate specimens (IPS e.max CAD) were randomly assigned to three dentifrice groups (n = 24): two charcoal-based (Carvvo (CVV), Curaprox Black is White (CBW)) and one conventional (Colgate Total 12 (C12)), the control. Toothbrushing simulations were performed for 5,000, 10,000, and 20,000 cycles. Roughness (Ra) and gloss (GU) were measured at baseline and after each cycle. Surface characterization was performed using three-dimensional profilometry and scanning electron microscopy (SEM), which also analyzed abrasive particles. Data were analyzed using two-way repeated measures ANOVA and Tukey's post-hoc test (α = 0.05).</p><p><strong>Results: </strong>Roughness was affected only by dentifrice type (p = 0.004), with CVV producing higher mean values than C12, and CBW not differing from the others. Brushing cycle (p = 0.164) and interaction (p = 0.095) were non-significant. Gloss was affected by dentifrice (p < 0.001), brushing cycle (p < 0.001), and their interaction (p < 0.001). Charcoal-based dentifrices caused gloss reductions (CVV: 80.77GU baseline to 67.41GU at 20,000 cycles; CBW: 86.39GU baseline to 69.62GU at 20,000 cycles). In contrast, C12 increased gloss, reaching up to 95.97GU at 20,000 cycles.</p><p><strong>Conclusions: </strong>Charcoal-based dentifrices significantly compromise the surface integrity of glazed lithium disilicate ceramics by increasing roughness and reducing gloss. Conventional dentifrices demonstrate no deleterious effect on ceramic surfaces and may even enhance gloss.</p><p><strong>Clinical relevance: </strong>Routine use of charcoal-based dentifrices may accelerate surface wear and reduce optical properties of ceramic restorations, potentially compromising longevity and esthetic outcomes.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"424"},"PeriodicalIF":3.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of ageing on surface properties and biofilm adhesion on denture base resin material fabricated by different manufacturing techniques.","authors":"Mirjana Perić, Sanja Petrović, Aleksandra Čairović, Tamara Vlajić Tovilović, Aleksandar Racić, Radmila Panajotović, Rade Živković, Biljana Miličić, Milena Radunović","doi":"10.1007/s00784-025-06488-x","DOIUrl":"https://doi.org/10.1007/s00784-025-06488-x","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the effects of hydrothermal ageing on biofilm adhesion and surface properties of denture base resins fabricated via different techniques.</p><p><strong>Materials and methods: </strong>Denture base resin samples were produced using the following manufacturing techniques: conventional cold polymerization (CP), heat polymerization (HP), CAD-CAM milling (CADm) and CAD-CAM printing (3D). The samples were tested before and after hydrothermal ageing (5000 cycles, 5 °C/55°C). The surface roughness was measured using atomic force microscopy (AFM), and the hydrophobicity was observed by measuring the water contact angle (WCA). Monomicrobial biofilm of Staphylococcus aureus and Candida albicans were formed and quantified before and after ageing using colony-forming units (CFUs/ml) and the MTT test. Statistical differences were evaluated using a two-way analysis of variance (ANOVA).</p><p><strong>Results: </strong>Two-way ANOVA showed that ageing significantly influenced the roughness (p = 0.002). The highest roughness values before and after ageing were measured for 3D resin samples. The WCA values of 3D resin samples changed after ageing (p = 0.018). Amount and metabolic activity of monomicrobial biofilms were increased during ageing on all four tested denture base resins.</p><p><strong>Conclusions: </strong>Ageing influenced the roughness and microbial adhesion on all tested denture base materials, regardless of the manufacturing method.</p><p><strong>Clinical relevance: </strong>Finding support replacing dentures every five years due to increased biofilm adhesion post ageing.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"423"},"PeriodicalIF":3.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical efficacy and safety of bipolar water as a therapeutic approach for oral candidiasis: a randomized controlled trial.","authors":"Peiru Zhou, Jiakun Fang, Yanhong Meng, Jiangli Zheng, Furong Yang, Mingming Xu, Xu Zhang, Hong Hua","doi":"10.1007/s00784-025-06500-4","DOIUrl":"10.1007/s00784-025-06500-4","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Oral candidiasis (OC) is a fungal infection caused primarily by Candida albicans. Treatments are associated with side-effects and toxicity. Bipolar water (BW), a novel functional water with antimicrobial, biofilm-disrupting, and wound-healing properties, offers a promising alternative therapy.This clinical trial aimed at evaluating the efficacy and safety of BW in OC treatment.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This randomized controlled trial enrolled 72 participants diagnosed with OC. Participants were randomized into four groups: BW mouthwash at two concentrations (HOCl at 40-55 mg/L, group 1; HOCl at 120 mg/L ± 20 mg/L, group 2), BW mouthwash (HOCl at 40-55 mg/L combined with nystatin tablets, group 3), and control group (CG; nystatin tablets and sodium-bicarbonate mouthwash). Efficacy was evaluated by clinical efficacy, mycological efficacy, and comprehensive efficacy (defined as a combination of clinical and mycological responses), and safety was assessed by adverse event (AE) monitoring. P-values were considered significant if &lt; 0.05. The treatment schedule was as follows: BW mouthwash (groups 1, 2, and 3) was used five times a day, nystatin was taken three times a day (group 3), and sodium-bicarbonate mouthwash was used three times a day in the control group.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;At 14 days, groups 1 and 2 demonstrated percent clinical response of 76.5% and 64.7%, respectively, which was comparable with that in the CG (64.7%). Values for mycological response were 47.1% and 37.5%, with clearance of 23.5% and 18.8%, respectively. The CG achieved a significantly higher percent clearance (76.5%). With respect to comprehensive efficacy, there was no significant difference between the three groups (88.2%, 82.4%, and 88.2% for groups 1, 2, and 3, respectively), but percent cure was higher in the CG (52.9%) than in group 1 (17.6%) and group 2 (11.8%). At 28 days, group 3 achieved the highest values for clinical response (87.5%) and cure (68.8%), marginally exceeding that for the CG (76.5% and 58.8%, respectively). There was no significant difference between the two groups with respect to clinical efficacy, mycologic efficacy, and comprehensive efficacy. BW was well-tolerated, with only mild AEs reported (e.g., oral irritation), which did not merit treatment discontinuation.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;BW mouthwash, as a standalone or adjunctive therapy with nystatin tablets, is a safe and efficacious treatment for OC, significantly alleviating clinical symptoms and improving the chance of cure.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Clinical relevance: &lt;/strong&gt;The findings of this study provide valuable evidence on the clinical benefits of BW in treating OC, and may contribute to the development of new therapeutic options for this common (but challenging) condition.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Clinical trial registration: &lt;/strong&gt;The trial was registered at the Chinese Clinical Trial Registry (ChiCTR) under the registration number Chi","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"421"},"PeriodicalIF":3.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and microbiological effectiveness of limosilactobacillus reuteri in supportive periodontal therapy: randomized clinical trial.","authors":"Magda Mensi, Eleonora Scotti, Silvia Marchetti, Annamaria Sordillo, Gianluca Garzetti, Stefano Calza, Mark J Buijs, Egija Zaura, Bernd W Brandt","doi":"10.1007/s00784-025-06508-w","DOIUrl":"https://doi.org/10.1007/s00784-025-06508-w","url":null,"abstract":"<p><strong>Objectives: </strong>The purpose of the present randomized clinical trial was to evaluate the clinical and microbiological effects of Limosilactobacillus reuteri probiotic therapy as an adjunct to Guided Biofilm Therapy (GBT) during supportive periodontal therapy (SPT) of patients with a history of stage III or IV and grade B or C periodontitis and residual pockets.</p><p><strong>Materials and methods: </strong>Forty-four systemically healthy patients were selected. Complete periodontal assessment was performed including Pocket Probing Depth (PPD), Bleeding on Probing (BOP), Presence of supragingival plaque (PI), Clinical Attachment Loss (CAL) and Recession (REC). Two sites per patient with PPD ≥ 6 mm or PPD of 5 mm with BOP were selected in two different quadrants as test sites. A session of full-mouth debridement was provided at baseline (T0), and patients were randomized to receive a 3-weeks treatment with lozenges containing probiotic or placebo. Periodontal parameters were taken at baseline (T0), 3 months (T2), and 6 months (T3). Microbiological samples from the test sites were taken at baseline (T0), 3 weeks (T1), 3 months (T2), and 6 months (T3).</p><p><strong>Results: </strong>Forty patients completed the study. Both groups showed a significant decrease in PPD, BOP, CAL, and number/percentage of residual pockets compared to baseline. However, no inter-group differences were noted. The test group showed a lower percentage of BOP at sites with plaque at T2 and T3. The microbiological analysis detected minimal proportion of L. reuteri in the periodontal pockets. No significant inter-group differences were detected in the red complex at any observation time. The subgingival microbial dysbiosis index (SMDI) revealed a decrease in dysbiosis from T0 to T1, followed by a slight increase in dysbiosis towards T3 for both groups. However, no significant differences were noted between the groups.</p><p><strong>Conclusion: </strong>In our cohort of patients, 3 weeks of bi-daily supplementation with lozenges containing L. reuteri in conjunction with a session of SPT did not provide any additional reduction in PPD or number/percentage of residual pockets and did not have a long-lasting effect on the subgingival biofilm microbial composition. However, patients receiving the probiotic had less bleeding at sites with plaque.</p><p><strong>Clinical relevance: </strong>Whilst L. reuteri cannot be recommended as a standard adjunctive therapy in SPT, it can be considered to reduce BOP levels in patients with poor plaque control.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"422"},"PeriodicalIF":3.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bleaching with biocatalysts and violet LED: a randomized clinical trial using a hybrid design with parallel-group and split-mouth approaches : Violet LED and biocatalysts in in-office whitening: a split-mouth trial.","authors":"Lara Maria Bueno Esteves, Carlos Alberto de Souza Costa, Luciano Tavares Angelo Cintra, Karen Milaré Seicento Aidar, Cristina de Mattos Pimenta Vidal, Alice de Oliveira Santos, André Luiz Fraga Briso","doi":"10.1007/s00784-025-06503-1","DOIUrl":"https://doi.org/10.1007/s00784-025-06503-1","url":null,"abstract":"<p><strong>Objective: </strong>To compare conventional 35% hydrogen peroxide whitening for 45 min with test therapies lasting 15 min using a polymeric scaffold, peroxidase, and violet LED.</p><p><strong>Materials and methods: </strong>Sixty patients were randomized into three groups (n = 20): TC45 (35% HP for 45'), T15 (35% HP for 15'), and TT (polycaprolactone scaffold + 35% HP with peroxidase for 15'). One hemiarch was irradiated with violet LED (L) (8 cycles of 1 min, with 30-second intervals). ΔE₀₀ and ΔW_ID were evaluated using a portable spectrophotometer. Spontaneous sensitivity (questionnaire) and provoked sensitivity (thermal test) were assessed at T1, T2, T3, and after 7, 14, and 90 days (three-way repeated measures ANOVA). pH and hydrogen peroxide (HP) consumption were assessed during the first session (one-way ANOVA).</p><p><strong>Results: </strong>The TT group showed ΔE₀₀ efficacy similar to TC45 and superior to T15. With LED, the TTL group was equivalent to TCL45 and superior to TL15. Regarding ΔW_ID, TT was also comparable to TC45 and superior to T15, while TCL45 showed the best results with LED. LED irradiation significantly affected only the T15 groups. TC45 and TCL45 showed higher sensitivity, whereas TT and TTL exhibited lower pH drop and greater hydrogen peroxide degradation.</p><p><strong>Conclusion: </strong>Therapies using scaffold and peroxidase, with or without LED, were effective, safe, and less sensitive, representing promising alternatives to conventional whitening, even with a two-third reduction in application time.</p><p><strong>Clinical relevance: </strong>Whitening therapies using polymeric scaffold and peroxidase, with or without violet LED, are effective, safe, and cause less sensitivity, representing promising alternatives to conventional whitening.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"419"},"PeriodicalIF":3.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison between venous blood sampling and capillary volumetric absorptive microsampling for antibiotics levels monitoring in individuals with and without periodontal disease.","authors":"Ioanna Lazaridi, Eva Choong, Thomas Mercier, Laurent A Decosterd, Catherine Giannopoulou, Alkisti Zekeridou","doi":"10.1007/s00784-025-06466-3","DOIUrl":"https://doi.org/10.1007/s00784-025-06466-3","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;We aimed to compare the antibiotic concentrations obtained using the volumetric absorptive microsampling (VAMS) devices with those determined in plasma from conventional venous blood collected within the frame of a pharmacokinetic study of amoxicillin (AMO), metronidazole (MET), azithromycin (AZI), commonly used for periodontal treatment. The suitability and overall, acceptability of the VAMS approach was also ascertained by both participants of the pilot study and dentist practitioners.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;Twelve volunteers (6 subjects without periodontal problems (PH), and 6 individuals affected with periodontitis (PP)) were administered 500 mg each of amoxicillin, metronidazole, and azithromycin. Paired venous blood (VB) and capillary VAMS samples were collected at 2-, 6-,10-, 24-, 48- and 96-hours post-antibiotics administration. Antibiotic concentrations were determined using multiplex liquid chromatography coupled tandem mass spectrometry (LC-MS/MS). Statistical analyses included Mann-Whitney U tests and t-tests.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Significant differences in antibiotic concentrations were observed between VAMS and venous blood (VB) collection methods, across different time points for the three antibiotics (p &lt; 0.05). AMO concentrations in VB were 3.5-fold higher (p &lt; 0.01) than in VAMS at early time points (2, 6, 10 h (h)). MET levels in VB were 1.5-fold higher than in VAMS at 2 h and 6 h, (p &lt; 0.01), but this difference disappeared after 10 h. Alternately, while AZI levels were similar in VB and VAMs 2 h after administration, AZI concentrations in VB and VAMS declined non parallelly, with VB levels decreasing to about 60 to 25% of those measured in VAMS over the observed 96 h interval. Antibiotic exposures were not different in the PH and PP groups. Differences in antibiotics concentrations determined in VB and VAMS samples are a direct consequence of (i) the matrices used for analyses (plasma in VB, vs. whole blood with VAMS), (ii) the subjects' hematocrit, and (iii) the distinct cell distribution pattern of antibiotics with AMO characterized by a weak penetration in red blood cells (RBC) while AZI tends to progressively concentrate into RBC. MET was present at higher concentrations in plasma until 6 h which thereafter tended to re-equilibrate equally in plasma and RBC.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Though VAMS yielded significantly different results compared to plasma, it effectively reflects the concentration evolution of the antibiotics and could be an alternative in pharmacokinetic studies and therapeutic monitoring.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Clinical relevance: &lt;/strong&gt;VAMS holds promise in advancing therapeutic drug monitoring in periodontal research and clinical practice. Being less invasive than venous puncture it is well accepted by subjects and facilitate blood monitoring in clinical trials and non-hospital settings. Its minimal invasiveness and simplified logistic","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 9","pages":"420"},"PeriodicalIF":3.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}