Clinical Oral Investigations最新文献

{"title":"Segmenting beyond the imaging data: creation of anatomically valid edentulous mandibular geometries for surgical planning using artificial intelligence.","authors":"Stefan Raith, Tobias Pankert, Srikrishna Jaganathan, Kristina Pankert, Hyun Lee, Florian Peters, Frank Hölzle, Ali Modabber","doi":"10.1007/s00784-025-06471-6","DOIUrl":"10.1007/s00784-025-06471-6","url":null,"abstract":"<p><strong>Background and objectives: </strong>Mandibular reconstruction following continuity resection due to tumor ablation or osteonecrosis remains a significant challenge in maxillofacial surgery. Virtual surgical planning (VSP) relies on accurate segmentation of the mandible, yet existing AI models typically include teeth, making them unsuitable for planning of autologous transplants dimensions aiming for reconstructing edentulous mandibles optimized for dental implant insertion. This study investigates the feasibility of using deep learning-based segmentation to generate anatomically valid, toothless mandibles from dentate CT scans, ensuring geometric accuracy for reconstructive planning.</p><p><strong>Methods: </strong>A two-stage convolutional neural network (CNN) approach was employed to segment mandibles from computed tomography (CT) data. The dataset (n = 246) included dentate, partially dentate, and edentulous mandibles. Ground truth segmentations were manually modified to create Class III (moderate alveolar atrophy) and Class V (severe atrophy) models, representing different degrees of post-extraction bone resorption. The AI models were trained on the original (O), Class III (Cl. III), and Class V (Cl. V) datasets, and performance was evaluated using Dice similarity coefficients (DSC), average surface distance, and automatically detected anatomical curvatures.</p><p><strong>Results: </strong>AI-generated segmentations demonstrated high anatomical accuracy across all models, with mean DSCs exceeding 0.94. Accuracy was highest in edentulous mandibles (DSC 0.96 ± 0.014) and slightly lower in fully dentate cases, particularly for Class V modifications (DSC 0.936 ± 0.030). The caudolateral curve remained consistent, confirming that baseline mandibular geometry was preserved despite alveolar ridge modifications.</p><p><strong>Conclusions: </strong>This study confirms that AI-driven segmentation can generate anatomically valid edentulous mandibles from dentate CT scans with high accuracy. The innovation of the work is the precise adaptation of alveolar ridge geometry, making it a valuable tool for patient-specific virtual surgical planning in mandibular reconstruction.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"501"},"PeriodicalIF":3.1,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic accuracy of aMMP-8 levels in oral biofluids for monitoring periodontitis in patients with metabolic syndrome.","authors":"Julie Toby Thomas, Betsy Joseph, Ismo T Räisänen, Sukumaran Anil, Tuomas Waltimo, Timo Sorsa","doi":"10.1007/s00784-025-06584-y","DOIUrl":"10.1007/s00784-025-06584-y","url":null,"abstract":"<p><strong>Objectives: </strong>The increasing prevalence of metabolic syndrome (MetS) and periodontitis, both marked by chronic inflammation that disrupts immune function, underscores the need for tailored healthcare approaches that account for their systemic inflammatory impact. The study aimed to estimate and compare the diagnostic accuracy of aMMP-8 expression in oral rinse, saliva, and GCF (gingival crevicular fluid) among patients with periodontitis and those with and without MetS.</p><p><strong>Materials and methods: </strong>A pilot sample of 77 participants was categorized into three groups based on the 2018 AAP/EFP periodontitis classification and the 2006 IDF metabolic syndrome criteria: MetS with periodontitis (MetS-PD; n = 34), systemically healthy with periodontitis (SH-PD; n = 21), and healthy controls (SH-PH; n = 22). Active metalloproteinase-8 (aMMP-8) levels in oral biofluids were quantified using an enzyme-linked immunosorbent assay. ANOVA and ROC curve analysis were done to assess diagnostic accuracy across groups.</p><p><strong>Results: </strong>aMMP-8 levels varied by group but remained consistent across biofluids, with lower levels in SH-PH and higher levels in SH-PD and MetS-PD (p < 0.001). aMMP-8 levels were significantly elevated in MetS-PD (25.86 ± 4.48 ng/ml) and SH-PD (24.72 ± 2.13 ng/ml) compared to controls (11.99 ± 3.46 ng/ml) in oral rinse samples (p < 0.001). Oral rinse demonstrated superior diagnostic accuracy (AUC = 0.89) compared to saliva (AUC = 0.85) and GCF (AUC = 0.82) in distinguishing MetS-PD from healthy controls, with an optimal cut-off value of 20 ng/ml.</p><p><strong>Conclusion: </strong>Oral rinse may be a preferable matrix for diagnosing periodontal disease, particularly in patients with systemic conditions, compared to saliva and GCF. Prospective longitudinal studies are required to evaluate its utility for monitoring over time.</p><p><strong>Clinical relevance: </strong>This study highlights the potential of aMMP-8 as a reliable biomarker for assessing periodontal disease severity in patients with MetS, utilizing non-invasive oral fluids. With oral rinses demonstrating high diagnostic accuracy, they may be an effective and accessible tool for screening and case-finding in medical and dental settings in patients with MetS, facilitating early detection and tailored management strategies. aMMP-8 immunoassay chair-side kit offers a practical alternative to traditional methods for screening patients in non-dental settings.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"500"},"PeriodicalIF":3.1,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verification of the relationship between awareness of clenching or the teeth contacting habit and the integral value of masseteric electromyogram during diurnal wakefulness.","authors":"Tomoya Ishimaru, Taihiko Yamaguchi, Taishi Saito, Yu Nakagawa, Yoshinori Hattori, Takahiro Ono, Yoshiaki Arai, Yoko Hasegawa, Hiroshi Shiga, Katsushi Tamaki, Junko Tanaka, Kazuhiro Tsuga, Hitoshi Abekura, Shouichi Miyawaki, Aya Maeda-Iino, Saki Mikami, Akihito Gotouda, Koichi Shimizu, Yuji Kato, Takeshi Namita, Shinichiro Kuroshima","doi":"10.1007/s00784-025-06566-0","DOIUrl":"https://doi.org/10.1007/s00784-025-06566-0","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to clarify the relationship between diurnal awake bruxism (AB) awareness, tooth-contacting habit (TCH) awareness, and the electromyographic (EMG) waveform integral value.</p><p><strong>Materials and methods: </strong>Unilateral masseteric EMG recordings during the daytime were performed on the habitual masticatory side using an ultraminiature wearable EMG device. One hundred and seven participants (50 and 57 with and without diurnal tooth clenching (d-TC) awareness, respectively) were included. d-TC awareness and TCH awareness were assessed using questionnaires. The standardized burst integral (S-integral) value was calculated for each participant.</p><p><strong>Results: </strong>There was a statistically significant association between d-TC awareness and TCH awareness. The S-integral values were not significantly associated with the awareness d-TC or TCH. The frequency distribution of EMG waveforms of the awareness and non-awareness groups exhibited wide-range overlaps both for d-TC and TCH.</p><p><strong>Conclusion: </strong>d-TC or TCH awareness showed no clear correlation with the S-integral value, suggesting that it is important to evaluate AB and TCH based on objective and quantitative test results, such as the masseter muscle's EMG data.</p><p><strong>Clinical relevance: </strong>Diagnosis, treatment, and management of AB and TCH are important issues in dental medicine. The obtained results regarding the actual state of awareness of AB and TCH, and the EMG integral value that was one of the important EMG indices, were considered useful information for establishing diagnostic and assessment methods for AB and TCH in future clinical dentistry.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"502"},"PeriodicalIF":3.1,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional facial volumetric changes in transgender individuals induced by gender-affirming hormone therapy: a stereophotogrammetric assessment.","authors":"Alex Moreira Mélo, Caio Sberni Pinheiro de Souza, Melissa de Oliveira Melchior, Manoel Damião de Sousa-Neto, Fabiane Carneiro Lopes-Olhê, Sérgio Henrique Pires Okano, Lúcia Alves da Silva Lara, Laís Valencise Magri, Jardel Francisco Mazzi-Chaves","doi":"10.1007/s00784-025-06577-x","DOIUrl":"https://doi.org/10.1007/s00784-025-06577-x","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate facial volumetric changes in transgender (trans) individuals undergoing gender-affirming hormone therapy (GAHT) using stereophotogrammetry (Vectra H1).</p><p><strong>Methods: </strong>This longitudinal observational study enrolled a total of 30 participants: 17 self-identified as transgender men, one as a non-binary transmasculine individual, and 13 as transgender women. All were receiving care at the Gender Diversity Gynecology Clinic (GDIG) of the Hospital das Clínicas, Ribeirão Preto Medical School, University of São Paulo (HCFMRP-USP). Facial scans were obtained at four time points: baseline (T0), 3 months (T1), 6 months (T2), and 12 months (T3) after initiation of GAHT. Volumetric analyses focused on five facial regions: mandibular, maxillary, frontal, mental (chin), and labial. Statistical analysis included the Mann-Whitney U test to compare groups and Spearman's correlation to assess associations between weight variation and facial volume changes. A multivariate analysis of covariance (MANCOVA) was conducted with facial volumetric regions as dependent variables, followed by post-hoc univariate ANCOVA.</p><p><strong>Results: </strong>Gender had a significant global multivariate effect on the combined set of facial volumes at all time points (p < 0.001). Trans men showed a consistent and significant increase in total facial volume and in all five regions over time, particularly in the lower third of the face. The pattern of change was rapid and homogeneous, with the most notable increases observed between T0 and T1 and sustained through T3. In contrast, trans women exhibited more variable and localized changes. Increases in maxillary, frontal, and labial volumes were observed at T1 and T2, with more widespread volume gains in four of five regions at T3, suggesting gradual adipose redistribution induced by estradiol.</p><p><strong>Conclusion: </strong>GAHT produces consistent global differences in facial volumetric patterns between trans men and trans women, with the most pronounced region-specific changes observed in total facial and mandibular volumes. Testosterone promotes a faster and more uniform masculinization of facial structures, while estrogen associated with an antiandrogen leads to more subtle and regionally distributed feminization over time. These findings demonstrate the effect of GAHT in promoting facial characteristics aligned with gender identity in transgender individuals, with distinct patterns of change observed between transgender men and women.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"493"},"PeriodicalIF":3.1,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting long-term success and survival in non-surgical root canal retreatment; a retrospective cohort study.","authors":"Ceren Turan Gökduman, Burhan Can Çanakçi, Esra Arili Öztürk, Özgür Er","doi":"10.1007/s00784-025-06592-y","DOIUrl":"https://doi.org/10.1007/s00784-025-06592-y","url":null,"abstract":"<p><strong>Objectives: </strong>This retrospective study aimed to evaluate the long-term (≥ 5 years) survival and success rates of nonsurgical root canal retreatment (NSRT) and to determine the prognostic factors associated with these outcomes.</p><p><strong>Materials and methods: </strong>A total of 943 teeth from 740 patients treated with NSRT by two experienced endodontists between January 1, 2015, and January 1, 2020, were reviewed. Based on strict inclusion and exclusion criteria, 408 teeth (376 patients) were included. Clinical and radiographic follow-ups were conducted. Periapical healing was assessed using the periapical index (PAI) by two calibrated examiners. Tooth retention was defined as survival, regardless of periapical status. Treatment success was determined using combined clinical and radiographic criteria. Multivariate logistic regression analysis was used to identify significant predictors.</p><p><strong>Results: </strong>A total of 408 teeth (376 patients) that met the inclusion criteria were included. The survival and success rates were 73.5%. Among surviving teeth, the success rate was 79%, after a follow-up of 78.5 ± 10.5 months. Restoration type (p < 0.001) and initial root canal obturation quality (p = 0.007) significantly influenced survival. Root or crown fractures accounted for 66.7% of extractions, with restoration type significantly related to extraction reason (p < 0.001). Success was significantly associated with pre-NSRT obturation quality (p = 0.035) and restoration quality (p < 0.001).</p><p><strong>Conclusions: </strong>Coronal restoration quality and initial obturation status appear to be important predictors of long-term NSRT outcomes.</p><p><strong>Clinical relevance: </strong>Observational data from routine endodontic practice help clarify long-term retreatment outcomes by revealing survival and success rates and identifying possible prognostic factors over a follow-up of at least five years.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"491"},"PeriodicalIF":3.1,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations among self-reported oral health and intrinsic capacity domains in U.S. older adults: analysis from NHANES 2009-2014.","authors":"Vittorio Dibello, Silvano Quarto, Frank Lobbezoo, Karl G H Parisius, Madia Lozupone, Filippo Santarcangelo, Claudia Chiapparino, Antonio Daniele, Antonio Dibello, Vincenzo Solfrizzi, Francesco Panza","doi":"10.1007/s00784-025-06601-0","DOIUrl":"https://doi.org/10.1007/s00784-025-06601-0","url":null,"abstract":"<p><strong>Objective: </strong>The World Health Organization suggested a framework for intrinsic capacity (IC), a construct central to healthy aging, emphasizing five domains: cognition, vitality, locomotion, sensory function, and psychological well-being. Although oral health may influence these domains, it remains underexplored within the IC model. The present study was the first to comprehensively examine the associations among self-reported oral health and IC domains.</p><p><strong>Methods: </strong>We analyzed data from 845 adults aged ≥ 60 years from the 2009-2014 National Health and Nutrition Examination Survey (NHANES), representing approximately 9.10 million non-institutionalized U.S. individuals aged ≥ 60 years. Self-reported oral health indicators included symptoms, treatment history, and hygiene behaviors. IC domains were approximated using conceptually aligned variables available in NHANES.</p><p><strong>Results: </strong>Perceived gum disease and reported bone loss were inversely associated with unintentional weight loss (vitality) [odds ratio (OR) = 0.54; 95% confidence interval (CI):0.30-0.96 and OR = 0.56; 95%CI:0.35-0.90, respectively]. Regular flossing and visible tooth abnormalities were linked to lower (OR = 0.59; 95% CI:0.35-0.98) and increased risk of impaired locomotion (OR = 2.38; 95% CI:1.19-4.76), respectively. Reporting teeth becoming loose without injury, visible tooth abnormalities, and regular flossing were associated with cognitive impairment, but not in fully-adjusted models. No significant associations were observed for mouthwash use or having ever received treatment for gum disease.</p><p><strong>Conclusion: </strong>Self-reported oral health indicators were associated with several IC domains in older adults.</p><p><strong>Clinical relevance: </strong>These findings highlighted oral health as a relevant factor in functional aging and supported its inclusion in comprehensive assessments aimed at promoting autonomy and well-being in later life.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"492"},"PeriodicalIF":3.1,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of disinfection and tissue repair with chloramphenicol-tetracycline-ZOE paste on pulp therapy of primary teeth: a systematic review.","authors":"José Antonio Santos Souza, Renata de Oliveira Alves, Priscila Toninatto Alves de Toledo, Heloisa Caroline Mota, Tamires Passadori Martins, Marcelle Danelon, Cristiane Duque, Gabriel Pereira Nunes","doi":"10.1007/s00784-025-06603-y","DOIUrl":"10.1007/s00784-025-06603-y","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review evaluated the effectiveness of the disinfection and tissue repair technique using chloramphenicol-tetracycline-zinc oxide eugenol paste (CTZ) in pulp therapy of primary teeth.</p><p><strong>Methods: </strong>Following PRISMA 2020 guidelines and PROSPERO registration, comprehensive searches were conducted in multiple databases. Eligibility criteria included clinical studies evaluating CTZ paste in children with pulp necrosis in primary teeth. Risk of bias was assessed using the Cochrane tool for randomized trials and the Newcastle-Ottawa Scale for non-randomized studies. Certainty of evidence was assessed using GRADE.</p><p><strong>Results: </strong>Ten studies, including 496 children aged 3-8 years, met the inclusion criteria. CTZ paste showed high clinical success rates (~ 90-100%) and radiographic success ranging from 72% to 100%. Compared to alternative treatments such as zinc oxide eugenol, 3Mix-MP, formocresol, Guedes-Pinto paste, and pulpectomy, CTZ showed similar outcomes in most comparisons. Additional advantages included reduced postoperative pain, simpler application, and lower procedural costs, suggesting its practicality in pediatric endodontics. Overall, studies presented a low risk of bias. Certainty of evidence was rated moderate for randomized trials and low for non-randomized studies.</p><p><strong>Conclusion: </strong>CTZ paste demonstrates potential as an effective and cost-efficient therapeutic option for the treatment of necrotic primary teeth, showing high clinical and radiographic success rates. Further well-designed, long-term randomized controlled trials are needed to confirm these findings.</p><p><strong>Clinical relevance: </strong>CTZ paste offers simplified, cost-effectiveness, and high success rates make it valuable in pediatric dentistry, especially for uncooperative children and in low-resource settings where conventional endodontic techniques may be unfeasible.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"488"},"PeriodicalIF":3.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qualitative and quantitative analysis of aerosol and droplet dispersion during simulated dental implant procedures using three types of instruments.","authors":"Hiromitsu Morishima, Tomonari Kajita, Jun Watanabe, Kenji Kikuchi, Yoko Iwamatsu-Kobayashi, Wataru Yashiro, Hiroyasu Kanetaka, Hiroshi Egusa, Kensuke Yamauchi","doi":"10.1007/s00784-025-06583-z","DOIUrl":"10.1007/s00784-025-06583-z","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the generation and dispersion dynamics of aerosols and droplets produced during dental procedures, including implant surgery.</p><p><strong>Materials & methods: </strong>Dental procedures were simulated on a test model using three different instruments: an air turbine handpiece, an ultrasonic device, and an implant motor. Particle behavior was visualized using two types of illumination light sources combined with a high-speed digital camera, enabling both qualitative and quantitative assessments of aerosol and droplet dispersion. Additionally, droplet deposition on water-sensitive paper placed in three different locations was analyzed to compare dispersion patterns among the three instruments.</p><p><strong>Results: </strong>The air turbine handpiece produced the highest luminance intensity (mean ± SD: 112.3 ± 6.4 a.u., n = 9), which was significantly greater than that of the implant motor (78.5 ± 5.2 a.u., n = 9; p < 0.05). For all devices, droplet diffusion was lower during molar treatment than during anterior tooth procedures. Water-sensitive paper analysis revealed increased droplet deposition at the extraoral vacuum site when the vacuum was activated (air turbine: 62 droplets; ultrasonic device: 49 droplets; n = 3 trials each), whereas droplet counts decreased at the patient's forehead.</p><p><strong>Conclusions: </strong>Simulated implant surgery generated less droplet dispersion compared with other dental procedures. Furthermore, the use of an extraoral vacuum markedly reduced droplet spread during various dental treatments.</p><p><strong>Clinical relevance: </strong>These data support layered controls-judicious instrument selection and extraoral suction-to reduce exposure during aerosol-generating procedures. Findings derive from a standardized simulation and should be validated in clinical settings.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"489"},"PeriodicalIF":3.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers during early healing of extraction procedures: a prospective case series.","authors":"Alessandra Toti, Ada Kura, Elena Sticchi, Marco Duvina, Vanni Balestri, Franco Amunni, Paolo Tonelli, Lorenzo Di Cesare Mannelli, Carla Ghelardini, Ester Parisi","doi":"10.1007/s00784-025-06534-8","DOIUrl":"10.1007/s00784-025-06534-8","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to: (1) evaluate the temporal profiles of VEGF-A, IL-1β, IL-6, IL-10, G-CSF, TNF-α, and β-NGF in surgical wound exudate (WF), comparing them to each patient's baseline values from gingival crevicular fluid (GCF); (2) describe a non-invasive, reliable method for collecting and processing WF to identify molecular markers involved in wound healing.</p><p><strong>Materials and methods: </strong>Twenty-four patients underwent surgical tooth extraction. GCF samples were collected preoperatively (T0), and WF samples 24 h (T1) and 7 days (T2) postoperatively. Protein concentrations were measured using multiplex ELISA and expressed as mean ± SEM.</p><p><strong>Results: </strong>IL-1β significantly increased from 23.58 ± 4.52 (T0) to 83.95 ± 21.39 (T1; p < 0.001). TNF-α rose from 1.82 ± 0.48 to 40.88 ± 11.27 (p < 0.0001), IL-6 peaked at 214.76 ± 38.21 (from 2.37 ± 0.98; p < 0.0001), and VEGF-A increased to 18.50 ± 4.85 (from 3.46 ± 2.19; p < 0.001).</p><p><strong>Conclusions: </strong>The method enabled efficient, non-invasive assessment of protein levels relative to individual baselines, confirming expected biomarker trends in early wound healing.</p><p><strong>Clinical relevance: </strong>Non-invasive biomarker analysis of wound exudate offers a practical approach to monitor healing and support targeted pain reduction and tissue repair strategies.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 11","pages":"490"},"PeriodicalIF":3.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional longitudinal assessment of nasal development with and without Nasoalveolar molding therapy in cleft lip and palate patients.","authors":"Lucas M Ritschl, Klaudia Narbekovas, Denys J Loeffelbein, Alex Grabenhorst, Nils Krautkremer, Hannes Singer, Andrea Grandoch, Helena Kram, Klaus-Dietrich Wolff, Florian D Grill","doi":"10.1007/s00784-025-06589-7","DOIUrl":"10.1007/s00784-025-06589-7","url":null,"abstract":"<p><strong>Background: </strong>Grayson-type presurgical nasoalveolar molding (PNAM) is an established method in presurgical infant orthopedics. This study compared PNAM therapy with conventional palatal plates across cleft types, focusing on nasal morphology.</p><p><strong>Methods: </strong>Seventy-two patients with non-syndromic unilateral (UCLP) and bilateral cleft lip and palate (BCLP) were followed from birth to two years. Three-dimensional (3D) models of the perinasal area were obtained via cast-based scans or 3D photographs; right-sided UCLP cases were mirrored to the left. Two independent reviewers analyzed anatomical landmarks with excellent inter-rater reliability (ICC = 0.88). Initial and two-year measurements were compared between PNAM (NAM group) and conventional treatment (non-NAM group).</p><p><strong>Results: </strong>Of the 72 patients, 46 had UCLP and 26 BCLP. In BCLP, NAM therapy promoted greater columella elongation from 2.1 mm at T₀ to 5.2 mm at T₃ compared to a change from 2.4 mm to 3.5 mm in the non-NAM group (p = 0.300). In UCLP, NAM was also associated with increases in columella (p = 0.387) and improved columella deviation angles, from a median of 48.1° at T₀ to 85.6° at T₃, while non-NAM showed a modest increase from 70.6° to 79.1°, (p = 0.167) Significant reductions were observed cleft-side nostril length (p = 0.027) and width (p = 0.029).</p><p><strong>Conclusion: </strong>This study, using 3D imaging, demonstrates the clinically relevant capability of PNAM to improve nasal morphology compared to conventional treatment, potentially enhancing primary cheiloplasty outcomes. The longitudinal design three-dimensionally tracks preoperative and postoperative changes over two years, with further investigation needed for long-term stability.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"487"},"PeriodicalIF":3.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}