Clinical Oral Investigations最新文献

{"title":"Artificial intelligence for craniofacial and mandibular growth prediction: A systematic review.","authors":"Alessandro Polizzi, Stefano Martina, Salvatore La Rosa, Rosalia Leonardi","doi":"10.1007/s00784-026-07190-2","DOIUrl":"10.1007/s00784-026-07190-2","url":null,"abstract":"<p><strong>Objectives: </strong>The primary objective was to evaluate the predictive performance of artificial intelligence (AI) models for craniofacial and mandibular growth prediction. Secondary objectives were to assess methodological quality and identify research gaps.</p><p><strong>Materials and methods: </strong>Nine databases were searched through August 12, 2026, supplemented by manual searches. Human studies assessing AI-based models for craniofacial or mandibular growth prediction were included. Study characteristics, prediction intervals, AI approaches, performance metrics, and validation strategies were extracted. Risk of bias and applicability were assessed using PROBAST.</p><p><strong>Results: </strong>Twelve studies were included. Six assessed overall craniofacial growth, whereas six mandibular development. Prediction horizons ranged from ≤ 1.5 to approximately 10 years, with sample sizes of 33-639 participants. Predictive accuracy varied by anatomical region and methodological approach. Sex and malocclusion type were frequently used to define study populations but were not consistently incorporated as predictors, whereas ethnicity and skeletal maturation were rarely considered. Seven studies had overall high risk of bias and five unclear risk, mainly due to analytical limitations and inadequate validation.</p><p><strong>Conclusion: </strong>AI-based models show promising predictive performance; however, current evidence is insufficient to establish superiority over traditional statistical methods. Overall high or unclear risk of bias and substantial heterogeneity in study populations and datasets, prediction horizons, outcome definitions, and AI modelling approaches limit confidence in the findings. Standardized multicenter datasets and independent external validation are required before clinical implementation.</p><p><strong>Clinical relevance: </strong>AI-based growth prediction may support individualized orthodontic diagnosis and treatment timing, but current evidence does not support routine clinical use.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13635025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofluid based biochemical and clinicopathological profiling for the detection and stratification of oral potentially malignant disorders.","authors":"Ronak A Varyani, Mandavi Waghmare, Panchali Saha, C Murali Krishna, Muskan Bajaj, Pranali Mane, Aishwarya Kathale, Vaidehi Mundhe, Purva Gaikwad","doi":"10.1007/s00784-026-07201-2","DOIUrl":"10.1007/s00784-026-07201-2","url":null,"abstract":"<p><strong>Objectives: </strong>To assess the clinical applicability of Raman spectroscopy using serum and saliva for the identification and stratification of oral potentially malignant disorders (OPMDs) and to differentiate Oral Leukoplakia and Oral Submucous Fibrosis from tobacco habit controls.</p><p><strong>Materials and methods: </strong>This case control study was conducted on 60 patients divided into three groups: leukoplakia (L), Oral submucous Fibrosis (OSMF), and tobacco controls (TC). Serum and saliva samples were collected and analyzed using a confocal Raman spectroscope. Multivariate analysis using principal component analysis (PCA) and linear discriminant analysis (LDA) was performed to generate classification models.</p><p><strong>Results: </strong>Spectral variations were observed among the groups in both serum and saliva, particularly in protein, lipid, nucleic acid related bands. Serum-based models demonstrated higher classification accuracy (~ 87.5%) compared to saliva (~ 80%) in 2-Class analyses. Combined 3-Class models showed accuracies of 70% and 60% in serum and saliva respectively. Class-specific sensitivity, specificity and predictive values for the three-class serum and saliva models are reported.</p><p><strong>Conclusion: </strong>In this exploratory case-control study, Raman spectroscopy of serum and saliva distinguished tobacco controls from patients with Oral Leukoplakia or OSMF with moderate-to-good classification accuracy, and serum-based models outperformed saliva-based models. These preliminary findings suggest that RS warrants further investigation as a minimally invasive adjunct for classification of OPMDs; validation in larger, prospective, multicentre cohorts with longitudinal follow-up is required before any early-detection or screening role can be established.</p><p><strong>Clinical relevance: </strong>Raman spectroscopy-based classification might eventually support risk-based triage for biopsy in high-burden settings. The present cross-sectional case-control design demonstrates group discrimination only, and does not by itself establish a screening, early-detection, or longitudinal-monitoring role.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological effects of dental resin composites on human gingival fibroblasts: a systematic review of in vitro evidence.","authors":"Elmira Boloori, Dagmar E Slot, Bruno G Loos, Teun J de Vries, Fridus G A van der Weijden","doi":"10.1007/s00784-026-07149-3","DOIUrl":"10.1007/s00784-026-07149-3","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review summarized and synthesized the in vitro biological effects of resin composites (RCs) on human gingival fibroblasts (HGFs) to clarify their isolated cellular biocompatibility.</p><p><strong>Materials and methods: </strong>A systematic search was conducted in the MEDLINE/PubMed database using a PECOS framework to identify in vitro studies evaluating HGF responses to RCs. Two independent reviewers screened literature, extracted data, and assessed methodological quality (QUIN tool) and certainty of the evidence (GRADE framework).</p><p><strong>Results: </strong>Thirty-eight studies comprising 185 distinct experiments across eight outcome measures were included. Marked heterogeneity was observed regarding RC composition (7 categories), specimen geometry, cell lines, and experimental durations. When evaluating cellular survival and damage independently, 27% of cytotoxicity experiments reported significant cell damage/death, while 45% of cell viability assays demonstrated a decreased metabolic activity. DNA damage was prominent, with 50% of 18 experiments reporting significant alterations. Significant increases occurred in reactive oxygen species production (71%) and inflammatory mediator release (62%). Conversely, 85% of morphology experiments showed no significant alterations. The overall certainty of evidence was graded as low to moderate.</p><p><strong>Conclusion: </strong>Dental resin composites can induce notable, independent adverse biological responses in human gingival fibroblasts in vitro, characterized by oxidative stress, inflammatory mediator release, DNA damage, and compromised viability. While highlighting a clear potential for toxicity, the low-to-moderate certainty and lack of physiological simulations leave their direct clinical significance open to further investigation.</p><p><strong>Clinical relevance: </strong>Synthesized evidence indicates that dental RCs exert moderate in vitro biological effects on gingival fibroblasts, including DNA-damage and inflammatory responses.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13638730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adhesive-based protective strategies for enamel cracks during in-office bleaching and their effect on hydrogen peroxide penetration.","authors":"Letícia Condolo, Laryssa Mylenna Madruga Barbosa, Gabrielle Gomes Centenaro, Taynara S Carneiro, Michael Willian Favoreto, Alessandro D Loguercio","doi":"10.1007/s00784-026-07200-3","DOIUrl":"10.1007/s00784-026-07200-3","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate hydrogen peroxide (HP) penetration in teeth with enamel cracks and to investigate if adhesive-based strategies can reduce its permeability without compromising bleaching efficacy (BE).</p><p><strong>Materials and methods: </strong>Fifty human premolars (n = 10 per group) were allocated into five groups: unbleached sound teeth (control), bleached sound teeth, bleached teeth with enamel cracks without adhesive strategy (cracked enamel), and two groups with enamel cracks receiving adhesive protection prior to bleaching (three-step etch-and-rinse adhesive [3-stepER] and universal adhesive used in an etch-and-rinse strategy [UA-ER]). Enamel cracks were detected using transillumination and measured using ImageJ software. In the adhesive groups, phosphoric acid etching was followed by adhesive application before two bleaching sessions. HP penetration into the pulp chamber was quantified using UV-Vis spectrophotometry. Color was assessed at baseline and after 7 and 14 days using digital spectrophotometry. Final bleaching efficacy was calculated from the color change between baseline and day 14 (ΔE_ab, ΔE₀₀ and ΔWI_D). Data were analyzed using one-way ANOVA followed by Tukey's post hoc test (α = 0.05).</p><p><strong>Results: </strong>HP penetration differed among the bleached groups (p < 0.05). The sound group showed the lowest HP levels (0.21 ± 0.06 µg mL⁻¹), whereas the cracked enamel (0.36 ± 0.23 µg mL⁻¹) and 3-stepER (0.32 ± 0.11 µg mL⁻¹) groups showed similar intermediate values. The UA-ER strategy showed the highest HP penetration (1.81 ± 0.34 µg mL⁻¹; p < 0.05). No significant differences in BE were observed among the bleached groups.</p><p><strong>Conclusions: </strong>Neither adhesive-based strategy provided a protective effect against HP diffusion in teeth with enamel cracks. The 3-stepER strategy did not reduce HP penetration compared with unprotected cracked enamel, whereas the UA-ER strategy resulted in significantly greater HP penetration, indicating an adhesive-dependent response.</p><p><strong>Clinical relevance: </strong>The use of adhesive systems as a protective strategy during in-office bleaching may not effectively reduce HP diffusion in teeth with enamel cracks. Clinicians should exercise caution when adopting such approaches.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of serum amyloid A in Saliva of patients with internal carotid artery stenosis: a pilot study.","authors":"Vrbanić Vrankić, A Berišić, L Šimunović, I Lapić, S Želalić, I Šutej","doi":"10.1007/s00784-026-07202-1","DOIUrl":"10.1007/s00784-026-07202-1","url":null,"abstract":"<p><strong>Objectives: </strong>To determine whether serum amyloid A (SAA) is detectable in saliva of patients with internal carotid artery (ICA) stenosis and to investigate the relationship between salivary and serum SAA levels across the perioperative period of carotid endarterectomy (CEA).</p><p><strong>Materials and methods: </strong>Six patients with asymptomatic ICA stenosis (60-99%) undergoing CEA were enrolled. Periodontal status was assessed at enrolment and used to characterise the cohort and document potential oral inflammatory confounding of salivary SAA. Salivary and serum SAA, C-reactive protein (CRP), and lipid parameters were measured before CEA, on postoperative day 2, and 30-45 days postoperatively using enzyme-linked immunosorbent assay (ELISA) and standard laboratory methods.</p><p><strong>Results: </strong>SAA was detectable in all saliva samples. Serum SAA increased after surgery and did not decline at follow-up, whereas salivary SAA showed a progressive decline (repeated measures ANOVA: F(2,4) = 25.01, p = 0.005). A strong negative correlation was observed between changes in serum and salivary SAA (Pearson's r = - 0.976, p = 0.004).</p><p><strong>Conclusions: </strong>In this small pilot cohort, SAA was detectable in all saliva samples, and salivary concentrations decreased across the perioperative time points while serum SAA did not show a parallel decline. The observed inverse serum-saliva association should be interpreted cautiously given the very small sample size, absence of a control group, and potential oral and sampling confounders. These findings support the feasibility of measuring salivary SAA in this clinical setting and warrant confirmation in larger controlled studies.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apatite-like calcium phosphate deposition and physicochemical properties of an aspartic acid-functionalized hydraulic cement.","authors":"Elias Daniel Covas Rodrigues, Victor Miguel Polizeli, Júlia Tavares de Sousa, Larissa Fernanda Pereira, Regina Guenka Palma-Dibb, Larissa Moreira Spinola de Castro-Raucci, Marcelle Danelon, Walter Raucci-Neto","doi":"10.1007/s00784-026-07196-w","DOIUrl":"10.1007/s00784-026-07196-w","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated the effects of aspartic acid modification on the physicochemical properties and phosphate-associated surface response of a hydraulic calcium silicate cement.</p><p><strong>Materials and methods: </strong>White mineral trioxide aggregate (MTA) was mixed using 0%, 25%, 50%, 75%, or 100% (v/v) of a 4 g/L aspartic acid stock solution. Final setting time, solubility, pH, and Ca²⁺ concentration in the immersion solution were evaluated after seven days in deionized water (n = 10). Surface roughness, morphology, elemental composition, and phosphate- and carbonate-associated spectral features were assessed after 30 days of storage in deionized water or phosphate-buffered saline (PBS). The Ap/C spectral ratio was calculated from the integrated ATR-FTIR band areas. Data were analyzed using one- or two-way ANOVA followed by Tukey's test (α = 0.05).</p><p><strong>Results: </strong>The 25% formulation showed the shortest setting time, whereas the 100% formulation showed the longest (p < 0.001). All aspartic acid-containing formulations exhibited greater solubility and lower Ca²⁺ concentrations than the control (p < 0.05), and the 50%, 75%, and 100% formulations exceeded the recommended solubility limit. The 25% formulation had the lowest pH, whereas the control, 75%, and 100% formulations maintained similar alkaline values. Surface roughness was lowest for the 25% formulation and highest for the 100% formulation, with no significant effect of storage medium. EDS detected negligible surface phosphorus after storage in deionized water but marked phosphorus content after PBS storage. Ap/C analysis showed significant main effects of cement formulation and storage medium, without a significant interaction. The 25%, 50%, and 75% formulations exhibited higher marginal Ap/C ratios than the 0% and 100% formulations.</p><p><strong>Conclusions: </strong>Aspartic acid produced non-linear and formulation-dependent changes in the evaluated properties. Higher Ap/C ratios reflected differences in relative spectral composition but did not confirm increased apatite formation or a crystalline apatite phase. Ca-P-rich surface deposition depended on phosphate supplied by the storage medium, and no formulation improved all evaluated outcomes.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indications for endodontic surgery in private practice and temporal trends before and after adoption of laser-activated irrigation: a retrospective practice-based study.","authors":"David Han, Christopher Chun, Catherine Kim, Laurel Martinez, Jooyoung Song","doi":"10.1007/s00784-026-07199-7","DOIUrl":"10.1007/s00784-026-07199-7","url":null,"abstract":"<p><strong>Objectives: </strong>To characterize indications for endodontic microsurgery (EMS) and compare the relative proportion of these indications across two calendar periods before and after the practice's adoption of laser-activated irrigation (LAI).</p><p><strong>Materials and methods: </strong>A retrospective time-period comparison of consecutive EMS cases (n = 1,672) treated by six endodontists (April 2019-August 2025) was analyzed. Two periods were prespecified based exclusively on the date of surgery: Before-Laser Treatment (BLT) and After-Laser Treatment (ALT) following implementation of an Er, Cr: YSGG intracanal LAI protocol (August 2022). Each surgical tooth was assigned one or two predefined reasons. Proportions were compared between periods using Generalized Estimating Equations (GEE) adjusting for patient clustering, demographics, tooth class, and provider.</p><p><strong>Results: </strong>The most frequent indications overall were large post (22.2%), uncleaned accessory canal (21.8%), and excessive calcification (14.4%). In adjusted models, the proportion of EMS cases attributed to uncleaned accessory canals decreased from 26.6% (204/767) in BLT to 17.7% (160/905) in ALT (adjusted OR = 0.49; 95% CI 0.37 to 0.65; P < .001). Conversely, the proportion of EMS cases attributed to excessive calcification increased from 10.8% (83/767) to 17.3% (157/905) (adjusted OR = 1.65; 95% CI 1.21 to 2.25; P = .002).</p><p><strong>Conclusions: </strong>In this practice-based case series, the ALT period was associated with a lower proportion of EMS cases performed for uncleaned accessory canals. By contrast, the proportion of EMS cases attributed to excessive calcification was higher in the ALT period. Causal inference regarding LAI effectiveness is limited by the time-period design and the lack of non-surgical denominator.</p><p><strong>Clinical relevance: </strong>Following the adoption of laser-activated irrigation (LAI) in non-surgical endodontics, uncleaned accessory anatomy constituted a smaller relative share of subsequent microsurgical cases. Conversely, clinicians should anticipate a continued substantial burden of calcification- and post-related surgical challenges.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coadministration of fluoride, trimetaphosphate, and quercetin reduces dentin erosive wear in vitro.","authors":"Isabela Maria Passarela Gomes, Alberto Carlos Botazzo Delbem, Tamires Passadori Martins, Isabela Ferreira da Silva, Lucas Fernando Oliveira Tomáz Ferraresso, Letícia Cabrera Capalbo, Marília Afonso Rabelo Buzalaf, Douglas Roberto Monteiro, Juliano Pelim Pessan","doi":"10.1007/s00784-026-07177-z","DOIUrl":"10.1007/s00784-026-07177-z","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the effect of solutions containing quercetin (QC), sodium trimetaphosphate (TMP), and fluoride (F), in different combinations, on dentin erosive wear (DEW) in vitro.</p><p><strong>Materials and methods: </strong>Bovine root dentin blocks were randomly divided into seven experimental groups (n = 28/group): deionized water (control); 500 ppm F (500F); 1100 ppm F (1100F); 5000 ppm F (5000F); 1100 F + 0.03% QC (F + QC); 1100 F + 1% TMP (F + TMP); and 1100 F + 1% TMP + 0.03% QC (\"EXP\" - experimental). Blocks were treated twice/day and subjected to four daily erosive challenges for five days (0.05 M citric acid, pH 3.2, 90 s), either erosion only (ERO) or erosion plus abrasion by brushing (ERO + ABR). The samples were analyzed by profilometry and computed X-ray microtomography, and the data were analyzed by 2-way ANOVA followed by Fisher's LSD post hoc test.</p><p><strong>Results: </strong>Under both conditions, a dose-response relationship was observed between F concentration and all variables, with the lowest values for 5000F and EXP. Under ERO, only 5000F showed lower DEW than 1100F; under ERO + ABR, all TMP and/or QC-containing solutions reduced DEW compared with 1100F, with a similar trend for mineral loss and depth of mineral loss, most notably for 5000F and EXP.</p><p><strong>Conclusions: </strong>Adding QC or TMP to 1100F produced modest additional protection, while their combination yielded a significant additive effect comparable to that of a solution with a five-fold higher F concentration.</p><p><strong>Clinical relevance: </strong>Possibility of reducing fluoride concentration in preventive formulations with an effect similar to that of high-concentration formulations against DEW.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13634009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary microbiome responses to chlorhexidine and Coriandrum sativum essential oil mouthwashes in patients with periodontitis in a randomized 2-week clinical trial.","authors":"Alisa Kazarina, Anete Vaškevica, Dārta Elizabete Senkāne, Pawel Zayakin, Sigita Hušča, Kristīne Vīksne, Valdis Pirsko, Lība Sokolovska, Natalja Sergejeva, Ilva Nakurte, Laura Pastare, Stefan Ruhl","doi":"10.1007/s00784-026-07198-8","DOIUrl":"10.1007/s00784-026-07198-8","url":null,"abstract":"<p><strong>Objectives: </strong>To compare the short-term salivary microbiome effects of a Coriandrum sativum essential oil (CSEO) mouthwash, chlorhexidine (CHX) as a benchmark comparator, and water in patients with periodontitis.</p><p><strong>Materials and methods: </strong>In this randomized three-arm trial, participants with periodontitis rinsed twice daily for 14 days with water, CSEO, or 0.12% CHX. Preclinical anti-Porphyromonas gingivalis and fibroblast cytotoxicity assays informed CSEO concentration selection. Shotgun metagenomic sequencing was performed on 54 saliva libraries, two per participant, from 27 participants sampled at baseline and week 2 (H₂O n = 9, CSEO n = 8, CHX n = 10). Alpha diversity, within-subject Bray-Curtis change, and differential abundance were analysed longitudinally.</p><p><strong>Results: </strong>Both active agents inhibited P. gingivalis in vitro, with CHX showing greater potency. In the clinical trial, no statistically detectable difference between CSEO and water was observed for the primary species-level Bray-Curtis outcome (q = 0.413), alpha diversity (all q ≥ 0.259), or differential abundance. CHX showed greater species-level Bray-Curtis change than water (q = 0.020), lower species-level Simpson diversity (q = 0.014), and broad taxonomic restructuring.</p><p><strong>Conclusions: </strong>Under the tested formulation and exposure conditions, CSEO did not produce a statistically detectable group-level salivary microbiome shift relative to water. CHX provided a benchmark for broad ecological perturbation. The CSEO null finding does not exclude modest or heterogeneous effects in a larger cohort.</p><p><strong>Clinical relevance: </strong>No clinical benefit can be inferred from the absence of a detectable CSEO-associated microbiome shift. Larger studies integrating clinical, inflammatory, and direct functional outcomes are needed.</p><p><strong>Trial registration: </strong>ISRCTN79156900, retrospectively registered on 11 May 2026.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interdependence of dental parameters, mandibular mobility and static postural sway as well as load distribution in male subjects of advanced working age.","authors":"Christian W, Krüger D, Erbe C, Maurer-Grubinger C, Oremek G, Groneberg D A, Ohlendorf D","doi":"10.1007/s00784-026-07193-z","DOIUrl":"10.1007/s00784-026-07193-z","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Maintaining static postural sway as well as load distribution is an essential component of overall physical stability in older age. The temporomandibular system is a measurable influencing factor due to functional interdependencies, although it has not yet been sufficiently investigated with regard to sex- and age-specific differences. This cross-sectional analysis therefore aims to examine the association between occlusion, mandibular mobility and static postural sway as well as load distribution in men aged 51 to 60, taking into account body mass index (BMI) and mandibular mobility.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 98 healthy men, aged between 51 and 60 years (mean age: 55.9 ± 2.72 years), volunteered for the study. Their mean BMI was 27.01 ± 3.91 kg/m² (mean height: 1.81 ± 0.07 m; mean weight: 88 ± 14.13 kg). BMI classification was based on WHO criteria. Postural measurements were conducted using a pressure platform. Mandibular movements (laterotrusion, protrusion and mouth opening) were recorded using an axiograph. Additionally, a model analysis according to Schopf was performed (midline deviation, supporting zone, and transverse width differences in the maxilla and mandible on both the right and left; occlusion of the canines [3s] and first molars [6s] on both sides; overjet; and overbite). The significance level was set at 5%.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Dental model analysis revealed that no participant was classified as Angle Class I, (mean overjet 3.93 mm and mean overbite 3.44 mm). All subjects exceeded the normal ranges defined by the Schopf classification, and the majority (75%) presented with Angle Class II. No significant association was found between BMI and static postural sway and well as load distribution in the overall analysis (p &gt; 0.05). BMI subgroup analysis: In the normal-weight group, occlusion of the right first molar (6) exhibited a modest positive correlation with ellipse area (p ≤ 0.01), while occlusion of the right canine (3) exhibited a modest positive correlation with frontal sway (p ≤ 0.01). In the pre-obese group, only a weak negative correlation was observed between occlusion of the right first molar (6) and forefoot load (p ≤ 0.01). In the obese group, occlusion of the right canine (3) showed a strong positive correlation with rearfoot load (p ≤ 0.01) and right laterotrusion showed a significant correlation with sagittal sway (p ≤ 0.02; moderate). Mandibular mobility subgroups in relation to BMI: in participants with hypomobile mouth opening, BMI correlated positively with frontal sway (p ≤ 0.03, strong). In the group with normal right laterotrusion, increasing BMI was associated with significant increases in frontal and sagittal sway, as well as ellipse parameters (p ≤ 0.01-0.05; all weak). In individuals with hypermobile mandibular function, BMI showed a strong negative correlation with occlusion of the first molars (6) in the left forefoot (p ≤ 0.001), as well as ","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"30 10","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}