{"title":"氟西诺酮、prooroot MTA及其联合应用对人牙髓炎症及成牙分化的影响。","authors":"Suphachart Chayawatto, Chatvadee Kornsuthisopon, Pairoj Linsuwanont","doi":"10.1007/s00784-025-06380-8","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To compare the effect of fluocinolone acetonide (FA), mineral trioxide aggregate (MTA), and their combination on inflamed human dental pulp cells (HDPCs), focusing on cell viability, expression of inflammation and mineralisation-associated genes, and odontogenic differentiation.</p><p><strong>Methods: </strong>HDPCs were treated with 20 µg/mL lipopolysaccharide (LPS) alone or in combination with and 400 µM hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) for 6, 12, 24 and 48 h to induce inflammatory conditions. Normal and inflamed HDPCs were further treated with FA, MTA extraction medium, or a combination of MTA and FA. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of inflammatory and mineralisation-associated genes was evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Odontogenic differentiation was determined by alkaline phosphatase staining (ALP) and alizarin red S staining (ARS).</p><p><strong>Results: </strong>HDPCs pretreated with LPS and H<sub>2</sub>O<sub>2</sub> had reduced cell viability, increased expression of IL-1β and IL-6, decreased expression of ALP and DSPP, and reduced odontogenic differentiation. Treatment with FA alone or in combination with MTA improved these parameters, except for odontogenic differentiation, which was enhanced only in the FA-treated group.</p><p><strong>Conclusion: </strong>FA exerted anti-inflammatory properties and promoted odontogenic differentiation under the inflammatory milieus of HDPCs in this in vitro study.</p><p><strong>Clinical relevance: </strong>Under the simulated inflammatory condition of HDPCs, this in vitro study suggests the potential benefit of FA for anti-inflammatory and mineralisation enhancing properties.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 7","pages":"358"},"PeriodicalIF":3.1000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of fluocinolone acetonide, ProRoot MTA and their combination on inflammation and odontogenic differentiation of inflamed human dental pulp cells.\",\"authors\":\"Suphachart Chayawatto, Chatvadee Kornsuthisopon, Pairoj Linsuwanont\",\"doi\":\"10.1007/s00784-025-06380-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To compare the effect of fluocinolone acetonide (FA), mineral trioxide aggregate (MTA), and their combination on inflamed human dental pulp cells (HDPCs), focusing on cell viability, expression of inflammation and mineralisation-associated genes, and odontogenic differentiation.</p><p><strong>Methods: </strong>HDPCs were treated with 20 µg/mL lipopolysaccharide (LPS) alone or in combination with and 400 µM hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) for 6, 12, 24 and 48 h to induce inflammatory conditions. Normal and inflamed HDPCs were further treated with FA, MTA extraction medium, or a combination of MTA and FA. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of inflammatory and mineralisation-associated genes was evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Odontogenic differentiation was determined by alkaline phosphatase staining (ALP) and alizarin red S staining (ARS).</p><p><strong>Results: </strong>HDPCs pretreated with LPS and H<sub>2</sub>O<sub>2</sub> had reduced cell viability, increased expression of IL-1β and IL-6, decreased expression of ALP and DSPP, and reduced odontogenic differentiation. Treatment with FA alone or in combination with MTA improved these parameters, except for odontogenic differentiation, which was enhanced only in the FA-treated group.</p><p><strong>Conclusion: </strong>FA exerted anti-inflammatory properties and promoted odontogenic differentiation under the inflammatory milieus of HDPCs in this in vitro study.</p><p><strong>Clinical relevance: </strong>Under the simulated inflammatory condition of HDPCs, this in vitro study suggests the potential benefit of FA for anti-inflammatory and mineralisation enhancing properties.</p>\",\"PeriodicalId\":10461,\"journal\":{\"name\":\"Clinical Oral Investigations\",\"volume\":\"29 7\",\"pages\":\"358\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Oral Investigations\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00784-025-06380-8\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Oral Investigations","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00784-025-06380-8","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
The effect of fluocinolone acetonide, ProRoot MTA and their combination on inflammation and odontogenic differentiation of inflamed human dental pulp cells.
Objectives: To compare the effect of fluocinolone acetonide (FA), mineral trioxide aggregate (MTA), and their combination on inflamed human dental pulp cells (HDPCs), focusing on cell viability, expression of inflammation and mineralisation-associated genes, and odontogenic differentiation.
Methods: HDPCs were treated with 20 µg/mL lipopolysaccharide (LPS) alone or in combination with and 400 µM hydrogen peroxide (H2O2) for 6, 12, 24 and 48 h to induce inflammatory conditions. Normal and inflamed HDPCs were further treated with FA, MTA extraction medium, or a combination of MTA and FA. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of inflammatory and mineralisation-associated genes was evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Odontogenic differentiation was determined by alkaline phosphatase staining (ALP) and alizarin red S staining (ARS).
Results: HDPCs pretreated with LPS and H2O2 had reduced cell viability, increased expression of IL-1β and IL-6, decreased expression of ALP and DSPP, and reduced odontogenic differentiation. Treatment with FA alone or in combination with MTA improved these parameters, except for odontogenic differentiation, which was enhanced only in the FA-treated group.
Conclusion: FA exerted anti-inflammatory properties and promoted odontogenic differentiation under the inflammatory milieus of HDPCs in this in vitro study.
Clinical relevance: Under the simulated inflammatory condition of HDPCs, this in vitro study suggests the potential benefit of FA for anti-inflammatory and mineralisation enhancing properties.
期刊介绍：
The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.
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