Influence of ageing on surface properties and biofilm adhesion on denture base resin material fabricated by different manufacturing techniques.

IF 3.1 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Oral Investigations Pub Date : 2025-08-28 DOI:10.1007/s00784-025-06488-x

Mirjana Perić, Sanja Petrović, Aleksandra Čairović, Tamara Vlajić Tovilović, Aleksandar Racić, Radmila Panajotović, Rade Živković, Biljana Miličić, Milena Radunović

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引用次数: 0

Abstract

Objectives: To evaluate the effects of hydrothermal ageing on biofilm adhesion and surface properties of denture base resins fabricated via different techniques.

Materials and methods: Denture base resin samples were produced using the following manufacturing techniques: conventional cold polymerization (CP), heat polymerization (HP), CAD-CAM milling (CADm) and CAD-CAM printing (3D). The samples were tested before and after hydrothermal ageing (5000 cycles, 5 °C/55°C). The surface roughness was measured using atomic force microscopy (AFM), and the hydrophobicity was observed by measuring the water contact angle (WCA). Monomicrobial biofilm of Staphylococcus aureus and Candida albicans were formed and quantified before and after ageing using colony-forming units (CFUs/ml) and the MTT test. Statistical differences were evaluated using a two-way analysis of variance (ANOVA).

Results: Two-way ANOVA showed that ageing significantly influenced the roughness (p = 0.002). The highest roughness values before and after ageing were measured for 3D resin samples. The WCA values of 3D resin samples changed after ageing (p = 0.018). Amount and metabolic activity of monomicrobial biofilms were increased during ageing on all four tested denture base resins.

Conclusions: Ageing influenced the roughness and microbial adhesion on all tested denture base materials, regardless of the manufacturing method.

Clinical relevance: Finding support replacing dentures every five years due to increased biofilm adhesion post ageing.

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老化对不同工艺制备的义齿基托树脂材料表面性能和生物膜粘附力的影响。

目的：评价水热老化对不同工艺制备的义齿基托树脂生物膜粘附性和表面性能的影响。材料和方法：采用常规冷聚合（CP）、热聚合（HP）、CAD-CAM铣削（CADm）和CAD-CAM打印（3D）技术制备义齿基托树脂样品。在热液老化（5000次循环，5°C /55°C）之前和之后对样品进行测试。采用原子力显微镜（AFM）测定表面粗糙度，通过水接触角（WCA）测定疏水性。采用菌落形成单位（cfu /ml）和MTT试验对老化前后金黄色葡萄球菌和白色念珠菌的单微生物生物膜进行形成和定量。采用双向方差分析（ANOVA）评估统计差异。结果：双因素方差分析显示，老化对粗糙度有显著影响（p = 0.002）。测量了三维树脂样品老化前后的最高粗糙度值。3D树脂样品的WCA值在老化后发生变化（p = 0.018）。在老化过程中，四种义齿基托树脂的单微生物生物膜的数量和代谢活性均有所增加。结论：无论采用何种制作方法，老化都会影响义齿基托材料的粗糙度和微生物粘附力。临床相关性：由于老化后生物膜粘连增加，每五年寻找支持替代义齿。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Oral Investigations 医学-牙科与口腔外科

CiteScore

6.30

自引率

5.90%

发文量

484

审稿时长

3 months

期刊介绍： The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.