Clinical laboratory最新文献

{"title":"Clinical Investigation of Rods and Rings Pattern in Anti-Nuclear Antibody Test in Korean Patients.","authors":"Ha-Eun Cho, Kyung Sun Park, Young Jin Kim, Tae Sung Park, Sun Young Cho","doi":"10.7754/Clin.Lab.2024.241110","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241110","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the clinical significance of rods and rings (RR) immunofluorescence patterns in antinuclear antibody (ANA) testing by analyzing their clinical associations.</p><p><strong>Methods: </strong>A single-center retrospective analysis was conducted between January 2022 and December 2023, examining cases with RR patterns in ANA testing.</p><p><strong>Results: </strong>Among 40 cases (0.5%) showing RR patterns, 35% coincided with autoimmune diseases, 25% with infections, and 22.5% with other inflammatory conditions. In specimens with high-intensity fluorescence, 45% were as-sociated with infections, and 18% with autoimmune diseases. Elevated inflammatory markers were observed in 54.5% of cases with inflammatory manifestations.</p><p><strong>Conclusions: </strong>The findings suggest that RR patterns in ANA testing may serve as potential indicators of underlying inflammatory processes.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Composition, Antioxidant, and Anti-Alzheimer Potential of Acetone Extract of Tamarix Africana Seeds with Docking Approach: Cholinesterase Enzymes.","authors":"Esma A T Khodja, Mohamed S Bensaad, Ouided Benslama, Abd El H Khabtane, Rabah Arhab, Chawki Bensouici, Roua S Baty, Rokayya Sami, Mohammad A Alanazi, Naseh A Algehainy, Zeyad M Alharbi, Ashwaq M Al-Nazawi, Nada Alqadri, Abdulsalam A Noorwali","doi":"10.7754/Clin.Lab.2025.250115","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250115","url":null,"abstract":"<p><strong>Background: </strong>Bioinformatics, a relatively new discipline, is now widely used to characterize ADMET profiling and the pharmacological characteristics of real bio-molecules found in vegetal, helping in developing new drugs. The actual work aimed to simulate ADMET profile, pharmacological capacities, and cytotoxicity capacities of bioactive molecules identified in Tamarix africana seed extract.</p><p><strong>Methods: </strong>Anticholinesterase and free radical scavenging capacities with chemical composition and total phenol, total flavonoid, and hydrolyzable tannin of Tamarix africana extract seeds have been quantified. In addition, molecular docking methods were used against cholinesterase's enzymes. LC-MS analyses revealed that the presence of several compounds in T. africana extract were apigenin-7-O-glucoside, diosmin, neohespiridin, and rutin.</p><p><strong>Results: </strong>Acetone extract from seeds exhibited a large amount of phenolics, flavonoids, and hydrolyzable tannins of 703 ± 3.88 GAE/mg, 266.03 ± 3.09 QE/mg, and 533.77 ± 2.00 TAE/mg, respectively. T. africana extract had a significant inhibitory effect against DPPH (IC50 = 4.50 ± 0.66 µg/mL), which is lower than standards. However, extract had a modest impact against ABTS+ (IC50 = 25.45 ± 1.74 µg/mL). Acetone extract of seeds showed higher IC50 of AChE (IC50 = 102 ± 0.41 µg/mL), while the galantamine showed lower IC50 BChE (IC50 = 4.99 ± 1.33 µg/mL). In silico study revealed that the biocompounds tested have notable cytotoxic effect.</p><p><strong>Conclusions: </strong>Furthermore, these compounds may be pharmacologically useful. Molecular docking studies demon-strated that among the four tested plant-derived compounds, apigenin-7-O-glucoside, diosmin, neohesperidin, and rutin, apigenin-7-O-glucoside exhibited the highest binding affinity toward acetylcholinesterase (AChE), while diosmin showed the strongest interaction with butyrylcholinesterase (BChE). These findings underscore the therapeutic potential of selected plant bioactives as lead candidates in cholinesterase-targeted drug discovery. T. africana extract can be utilized as a possible source of substitute chemicals.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Characteristics of Respiratory Pathogens Among Children in the Hangzhou Region, China.","authors":"Suili Cai, Wenqing Xiang","doi":"10.7754/Clin.Lab.2025.240927","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.240927","url":null,"abstract":"<p><strong>Background: </strong>We aimed to evaluate the epidemiological characteristics of tested pathogens associated with respiratory infections in children.</p><p><strong>Methods: </strong>We included 164,778 patients aged 0 - 16 years who were diagnosed with respiratory tract infections (RTIs) between January 2021 and December 2023 at the Children's Hospital, Zhejiang University School of Medicine. A lateral flow assay (LFA) was used to detect tested respiratory pathogens: mycoplasma pneumoniae (MP), human adenovirus (HADV), influenza A virus (IFVA), influenza B virus (IFVB), and human respiratory syncytial vi¬rus (HRSV).</p><p><strong>Results: </strong>Among all patients, 59,243 (35.95%) were infected with at least one pathogen, 909 (1.53%) were co-infected with two pathogens, and 29 (0.05%) were infected with three. Except for HRSV, the positivity rate was higher in males than in females. IFVA had the highest detection rate across all age groups. The difference in viral infection positivity by age was statistically significant (χ² = 1,490.685, p < 0.05). IFVA and IFVB had the highest positivity rates in winter (26.48% and 12.87%, respectively), which showed statistical differences from the other three season groups (p < 0.05/6 = 0.008). The positivity rates of MP and HRSV were highest in autumn (1.93% and 4.64%, respectively).</p><p><strong>Conclusions: </strong>The epidemiological characteristics of respiratory pathogen infections were significantly associated with gender, age, season, and prior SARS-CoV-2 infection. MP, HADV, IFVA, and IFVB were more prevalent in males than females. Overall positivity increased with age and was highest in winter. Positivity rates for all viral infections, except IFVA, declined after SARS-CoV-2 restrictions were lifted. These insights are vital for public health strategies aimed at mitigating respiratory infections in children.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary Cutaneous Diffuse Large B-cell Lymphoma, Leg Type with Immune Thrombocytopenia as the First Primary Presentation.","authors":"Yingying Sun, Chunyan Liu, Huaquan Wang, Zonghong Shao","doi":"10.7754/Clin.Lab.2025.250156","DOIUrl":"10.7754/Clin.Lab.2025.250156","url":null,"abstract":"<p><strong>Background: </strong>Primary cutaneous diffuse large B-cell lymphoma, leg type (PCDLBCL-LT) is a rare aggressive vari-ant of non-Hodgkin lymphoma (NHL). It is also clinically uncommon for NHL to primarily manifest as immune thrombocytopenia (ITP).</p><p><strong>Methods: </strong>We report a rare case of PCDLBCL-LT, which was misdiagnosed as primary refractory ITP. He achieved a complete remission of ITP by cyclophosphamide. Five months later, the patient developed recurrent erythematous plaques that disseminated to his entire body, accompanied by extracutaneous lesions.</p><p><strong>Results: </strong>Skin biopsy indicated PCDLBCL-LT. After one cycle of CHOP regimen, the patient's condition improved.</p><p><strong>Conclusions: </strong>When encountering refractory ITP, it is important to identify potential underlying causes.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Automated Assay for Carbapenemase Detection in Enterobacterales: a Real-World Performance.","authors":"Yu Kyung Kim, Young Deok Park, Jung Ok Kim, Soon Hee Chang, Kyung Eun Song","doi":"10.7754/Clin.Lab.2025.250150","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250150","url":null,"abstract":"<p><strong>Background: </strong>Carbapenem-resistant Gram-negative organisms pose a significant challenge to global healthcare, making the reliable detection and classification of carbapenemases essential for informed therapy and infection control. The BD Phoenix CPO detect assay offers simultaneous carbapenemase detection and Ambler classification in routine antimicrobial susceptibility testing. Accordingly, this study analyzed real-world data on the BD Phoenix CPO test to evaluate its performance in clinical settings over a period of two years.</p><p><strong>Methods: </strong>We analyzed 298 samples using BD Phoenix CPO detect assay, Xpert Carba-R polymerase chain reaction (PCR), and conventional PCR from January 2021 through December 2022 to identify and confirm carbapenemase genes. The BD Phoenix system was used for antimicrobial susceptibility testing and carbapenemase classification. The Xpert Carba-R kit detected five key carbapenemase genes (blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48), while conventional PCR identified these five genes and additional five carbapenemase genes (blaGES, blaSPM, blaSME, blaSIM, and blaGIM). The PCR results were compared with those from the Phoenix system.</p><p><strong>Results: </strong>Sputum was the most common specimen type, and Klebsiella pneumoniae accounted for 86.9% of the identified species. The concordance between the BD Phoenix assay and PCR for identifying carbapenemase genes showed 98.8%, 35.3%, and 71.4% agreement for Ambler class A, B, and D, respectively. The Phoenix had a false positive rate of 9.0%. Additionally, 21.2% of carbapenemase-positive cases had uncertain classifications. The con-cordance between Carba-R PCR and conventional PCR was 98.4%, with positive and negative agreement of 98.2% and 86.2%, respectively.</p><p><strong>Conclusions: </strong>The BD Phoenix CPO test provides a significant advantage of delivering results concurrently with antimicrobial susceptibility testing, representing a highly valuable tool for routine screening in clinical practice. Nevertheless, its high false positive rate underscores the necessity of confirmatory testing to ensure accurate carbapenemase classification.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and QS Inhibition by Schinus molle Essential Oil: Chemical Profiling and Docking Against S. aureus.","authors":"Soumia Tabti, Djamila Boukraâ, Widad Hadjab, Oussama K Belhadj, Yahia Khelef, Pilar Truchado, Isabel Martínez-Alcalá, Ramazan Erenler, Rokayya Sami, Amal Alyamani, Fayez Alsulaimani, Ahmed M Basri, Roqayah H Kadi, Afnan M Alnajeebi","doi":"10.7754/Clin.Lab.2025.250461","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250461","url":null,"abstract":"<p><strong>Background: </strong>Owing to the worldwide issues of resistance to antibiotics and the role of quorum sensing in the regulation and development of bacteria (Staphylococcus aureus) virulence factors, scientific communities explore alternatives to drugs, such as medicinal plants' essential oils. This study was performed to evaluate the antivirulence, biofilm retardation, and quorum sensing (QS) retarding activities of Schinus molle essential oil (SMEO) against the pathogenic S. aureus and its Agr mutant strains and evaluate the antiquorum activity of S. molle EO compounds.</p><p><strong>Methods: </strong>The chemical composition of SMEO was measured using the Fourier-transform infrared spectroscopy (FT-IR) and gas chromatographymass spectrometry (GC-MS) approaches. Antimicrobial activity was studied using agar disc diffusion.</p><p><strong>Results: </strong>Notably, the results revealed 20 compounds with limonene (35.82%) and α-phellandrene (19.13%) dominant constituents. The antibacterial effects were assessed via the agar dilution method, while anti-QS activity was examined using Chromobacterium violaceum CECT 494. Remarkably, the SMEO exhibited strong bactericidal potentials, effectively destroying the bacterial cells within 24 hours duration with inhibition zones reaching up to (34.33 ± 0.1 mm). Additionally, the SMEO inhibited staphyloxanthin formation, exopolysaccharide (EPS), and biofilm formation with significant reduction up to 96.27%, 93.78%, and 92.87%, respectively. Likewise, slime production and motility were highly affected by SMEO. These were done with extraordinary declines witnessed in a concentration-dependent pattern. Furthermore, the molecular docking analysis affirmed strong collaborations be-tween the SMEO constituents and the AgrA and thereby supporting its antivirulence capability.</p><p><strong>Conclusions: </strong>Findings obtained from this study highlighted the promising prospects of SMEO as a potent natural compound in mitigating S. aureus virulence and biofilm formations, indicating its potential use as an alternative treatment to antibiotics.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Significance of CD109 Expression and Methylation Patterns in Acute Myeloid Leukemia.","authors":"Ting-Ting Du, Ming-Qiang Chu, Zi-Jun Xu, Xiang-Mei Wen, Jing-Dong Zhou, Wei Zhao, Min-Jie Zhang, Su-Wan Liu, Jun Qian, Jiang Lin","doi":"10.7754/Clin.Lab.2025.250128","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250128","url":null,"abstract":"<p><strong>Introduction: </strong>The altered expression of CD109 has been observed in solid tumors of various types, and evidence suggests a correlation between CD109 expression and development and prognosis of tumors. However, the expression and methylation patterns of CD109 in acute myeloid leukemia (AML) and their clinical significance remain unclear.</p><p><strong>Methods: </strong>CD109 mRNA expression and promoter methylation were determined in AML patients and leukemia cells. Furthermore, the clinical significance of the findings and the regulatory relationship between expression and methylation of CD109 were analyzed.</p><p><strong>Results: </strong>CD109 expression was found to be elevated in AML patients, both in publicly available datasets and in our own cohort. Subsequent clinical analysis showed an association between the wild-type form of NPM1 and higher CD109 expression. Kaplan-Meier survival analysis revealed that AML patients with higher CD109 expression had shorter overall survival times. Furthermore, the CD109 promoter was found to be hypomethylated in AML patients, and further analysis indicated that this hypomethylation led to elevated expression of CD109. We also observed that CD109 mRNA expression and demethylation were markedly decreased in samples from patients with complete remission compared to those from newly diagnosed patients.</p><p><strong>Conclusions: </strong>Our results suggest that CD109 exhibits elevated expression and hypomethylation in AML, with its expression being regulated by methylation of its promoter region. Furthermore, CD109 could serve as a prognostic indicator as well as a monitoring tool for the therapeutic outcome in AML.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Thiazole-Based Compounds as Potential Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 Inhibitors for Alzheimer's Disease.","authors":"Majid Alhomrani, Abdulhakeem S Alamri, Walaa F Alsanie, Abdulaziz Alsharif, Osama Abdulaziz, Magdi M Salih, Bassem M Rafat, Abdulwhab Alamri, Tahir A Chohan","doi":"10.7754/Clin.Lab.2025.241234","DOIUrl":"10.7754/Clin.Lab.2025.241234","url":null,"abstract":"<p><strong>Background: </strong>Alzheimer's disease is a neurodegenerative disorder that causes significant cognitive impairment and memory loss. It is the leading cause of dementia on a global scale and is distinguished by the pathological build-up of amyloid-beta peptides and tau protein. This study presents the development of E-pharmacophore modeling, which utilizes reported co-crystal structure involving beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) to screen the eMolecules database.</p><p><strong>Methods: </strong>The present study comprehensively dealt with the virtual screening and structure-based prediction of thiazole compounds against BACE1 protein. To investigate the binding mode of virtual-screened hits (VS-hits), top 100 VS-hits were docked into BACE1 followed by in silico ADMET prediction. Top two VS-hits (CP1 and CP2) with highest docking scores along with co-crystalized ligand (CPZ) were further subjected to MESP, HOMO, LUMO, MD simulation, and MMGBSA analysis to inspect the dynamic stability of inhibitor-BACE1 complexes and the key molecular interaction responsible for their improved binding affinity toward BACE1.</p><p><strong>Results: </strong>This research identified CP1 and CP2 as top two potential novel BACE1 inhibitors from the library of natural products, whose Glide docking scores range from -8.87 to -7.89 kcal/mol-1. Interestingly, both ligands were able to establish interactions with a set of conserved residues F108, I110, I118, L30, Q12, G13, G11, A335, S229, D228, G230, D32, G34, S35, and Y71. ADMET assessment of the selected compounds was also noted to be within acceptable ranges. The preliminary in-silico ADMET evaluation revealed encouraging results for all the modeled and in-house library compounds. The RMSD and RMSF analysis revealed that both ligands remained stable and maintained their interaction throughout the simulation time (100 nanoseconds). The MM/GBSA (ranging from -36.734 to -27.431 kcal/mol) predicted binding affinities are in strong correlation with that of the docking score, which not only supports the docking results but also suggests that CP1 exhibits superior binding affinity towards BACE1.</p><p><strong>Conclusions: </strong>Keeping in view these findings, CP1 might be a promising candidate for drug discovery against BACE1 inhibitors. The findings of this research have the potential to offer valuable recommendations for the advancement of novel, potent, and efficacious BACE1 inhibitors.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case of 8p12q11.21 Chimeric Duplication with Normal Pregnancy.","authors":"Weiping Chen, Tao Zhang, Caiping Chen, Liqing Gao","doi":"10.7754/Clin.Lab.2025.250154","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250154","url":null,"abstract":"<p><strong>Background: </strong>Chromosome 8 belongs to the C group of medium-sized sub-centromere chromosomes. Complete trisomy of 8 most often results in aborted embryos or spontaneous abortion. Chimeric trisomy 8 is a rare autosomal abnormality with high phenotypic heterogeneity.</p><p><strong>Methods: </strong>Peripheral blood DNA was extracted by magnetic bead method, and CNV-seq was performed with MGISEQ-2000 sequencer to analyze chromosome copy number variation (CNVs) in chromosome aneuploidy and those with more than 100 Kb.</p><p><strong>Results: </strong>The test result for the pregnant woman was sseq[GRCh37]8p12q11.21(32608173 48839569)x3 [0.77]chr8:g.32608173 48839569dup. The copy number variant type was pathogenic, and the fragment size was 16.23 Mb.</p><p><strong>Conclusions: </strong>This study suggests that 8p12q11.21 chimeric duplication carriers may have normal phenotype and reproductive function, which should be considered in genetic counseling, especially prenatal counseling.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolated Central Nervous System Relapse in a Patient with FLT3-ITD Positive Acute Promyelocytic Leukemia.","authors":"Xiaoyu Li, Jiang Zeng","doi":"10.7754/Clin.Lab.2025.250151","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250151","url":null,"abstract":"<p><strong>Background: </strong>Acute promyelocytic leukemia (APL) is a unique and highly manageable form of acute myeloid leukemia, typically identified by the presence of the PML-RARA fusion gene. However, relapse in the central nervous system (CNS) continues to be a rare but serious complication, particularly in patients with high-risk genetic features such as FLT3-ITD mutations. CNS involvement significantly complicates the course of APL and demands prompt recognition and management.</p><p><strong>Methods: </strong>We present the case of a 60-year-old male diagnosed with FLT3-ITD positive APL who developed CNS relapse. The patient underwent a comprehensive diagnostic workup, involving magnetic resonance imaging (MRI) and cerebrospinal fluid (CSF) evaluation using next-generation sequencing and flow cytometry. The patient was treated with intrathecal chemotherapy.</p><p><strong>Results: </strong>MRI revealed mild thickening and enhancement of the leptomeninges, suggestive of leptomeningeal in-volvement. CSF cytology and flow cytometry confirmed the presence of abnormal promyelocytes. The patient received intrathecal chemotherapy, which led to clinical improvement, with a reduction in the severity of headaches.</p><p><strong>Conclusions: </strong>CNS relapse in APL, though rare, requires high clinical suspicion, particularly in patients with FLT3-ITD mutations. Early diagnosis using imaging and CSF analysis, followed by prompt intrathecal therapy, is crucial for effective management. This case highlights the need for vigilance in monitoring high-risk APL patients for CNS complications and emphasizes the importance of early intervention to improve outcomes.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 8","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}