Clinical laboratory最新文献_第5页

The rSIG Score Predicts Intensive Care Unit Admission and Blood Transfusion in Pediatric Trauma Patients. rSIG评分预测儿科创伤患者重症监护病房入院和输血。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250930

Guleser Akpinar, Mehmet Koşargelir, Yaser Guney, Amine E Başibuyuk, Ahmet S Yilmaz, Arzu Kose, Selmani Doğan

{"title":"The rSIG Score Predicts Intensive Care Unit Admission and Blood Transfusion in Pediatric Trauma Patients.","authors":"Guleser Akpinar, Mehmet Koşargelir, Yaser Guney, Amine E Başibuyuk, Ahmet S Yilmaz, Arzu Kose, Selmani Doğan","doi":"10.7754/Clin.Lab.2025.250930","DOIUrl":"10.7754/Clin.Lab.2025.250930","url":null,"abstract":"Background: The aim of this study was to compare the values of the Age-Adjusted Pediatric Shock Index (SIPA), Reverse Shock Index (rSI), and the index obtained by multiplying the Reverse Shock Index by the Glasgow Coma Scale (rSIG) in predicting prognosis and transfusion requirements in pediatric multitrauma patients.Methods: In this retrospective observational study, 168 patients presenting with multiple pediatric trauma com-plaints to the Emergency Medicine Clinic of Başakşehir Çam and Sakura City Hospital between June 1, 2020, and June 1, 2022, were evaluated. Patients were divided into two groups based on their rSIG values: normal (n = 85) and abnormal (n = 83). rSIG classification was performed using age-specific cutoff points (1 - 6 years: ≤ 13.1, 7 - 12 years: ≤ 16.5, 13 - 17 years: ≤ 20.1). ROC analysis was performed for intensive care unit (ICU) admission, transfusion requirement, and surgical intervention.Results: The AUC value of rSIG in predicting ICU admission was 0.811 (95% CI: 0.719 - 0.903), which was significantly superior to rSI (AUC: 0.664) and SIPA (AUC: 0.623) (p < 0.001). The AUC value of rSIG in predicting transfusion requirement was 0.861 (95% CI: 0.767 - 0.956), while it was 0.610 (95% CI: 0.524 - 0.696) for surgical intervention. In the logistic regression analysis, each 1-point increase in rSIG reduced the odds ratio for ICU admission by 0.78 (OR: 0.780, 95% CI: 0.652 - 0.933, p = 0.006).Conclusions: rSIG demonstrated significantly higher discriminatory power than SIPA and rSI for predicting ICU admission. Although rSIG showed higher AUC values for transfusion requirement and surgical intervention, these differences did not reach statistical significance. Nevertheless, rSIG may serve as a practical tool for rapid risk stratification and to transfer decisions in emergency departments, particularly for identifying patients requiring ICU admission.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"1911-1918"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Elderly Female Systemic Lupus Erythematosus Patient Presented with Severe Agranulocytosis as Initial Symptom. 老年女性系统性红斑狼疮患者以严重粒细胞缺乏症为首发症状。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250961

Yanping Tu, Guoxing Ge, Yang Ge

{"title":"An Elderly Female Systemic Lupus Erythematosus Patient Presented with Severe Agranulocytosis as Initial Symptom.","authors":"Yanping Tu, Guoxing Ge, Yang Ge","doi":"10.7754/Clin.Lab.2025.250961","DOIUrl":"10.7754/Clin.Lab.2025.250961","url":null,"abstract":"Background: Agranulocytosis refers to a severe reduction in neutrophils (with an absolute value less than 0.5 x 109/L) in peripheral blood, and a small portion of acquired agranulocytosis is caused by systemic lupus erythematosus (SLE). SLE is a chronic autoimmune disease involving multiple systems, mainly occurring in young women. Its clinical manifestations include polyarthralgia and arthritis, Raynaud's syndrome, rashes on the cheeks and other parts, pleurisy or pericarditis, involvement of the kidneys or central nervous system, and autoimmune cytopenia.Methods: Blood routine and bone marrow routine tests were used to detect the cell proliferation condition in peripheral blood and bone marrow. Complement, immunoglobulin, etc. were detected by biochemical instrument, and antinuclear antibody (ANA), anti-dsDNA antibody, etc. were detected by immunofluorescence method.Results: Blood routine indicated a WBC of 0.49 x 109/L with neutrophil count 0.09 x 109/L. Bone marrow routine showed bone marrow nucleated cells decreased, granulocyte granules increased and thickened. Biochemistry indicators: immunoglobulin G (IgG), immunoglobulin A (IgA), C-reactive protein (CRP), amylase (AMY), aspartate transaminase (AST), lactate dehydrogenase (LDH) were elevated, and complement C3, complement C4, albumin, eGFR were decreased. ANA.IgG positive (+), anti-dsDNA antibody positive (++).Conclusions: This postmenopausal SLE patient presented with agranulocytosis as the initial symptom are rarely seen. Therefore, clinicians should enhance their understanding of SLE, conduct a comprehensive analysis of various clinical manifestations, and conduct relevant tests timely to reduce misdiagnosis.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"2030-2033"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carcinoembryonic Antigen Elevation in Bronchial Foreign Body Patients Initially Misdiagnosed as Lung Cancer. 支气管异物早期误诊为肺癌患者的癌胚抗原升高。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250950

Run Q Zhai, Jing X Liu, Guo Z Yi, Yin P Li, Feng R Guo, Shan N Wei, Shao Q Pang, Hui Wang, Xiao D Wang, Qing B Cai Jing J Yan

{"title":"Carcinoembryonic Antigen Elevation in Bronchial Foreign Body Patients Initially Misdiagnosed as Lung Cancer.","authors":"Run Q Zhai, Jing X Liu, Guo Z Yi, Yin P Li, Feng R Guo, Shan N Wei, Shao Q Pang, Hui Wang, Xiao D Wang, Qing B Cai Jing J Yan","doi":"10.7754/Clin.Lab.2025.250950","DOIUrl":"10.7754/Clin.Lab.2025.250950","url":null,"abstract":"Background: We report a case of chronic bronchial foreign body complicated by recurrent pulmonary infection with hemoptysis, initially misdiagnosed as lung cancer after laboratory tests revealed markedly elevated carcinoembryonic antigen levels.Methods: Laboratory examination, contrast-enhanced chest CT scan, electronic bronchoscopy, and ultra-thin bronchoscopy were performed, confirming a bronchial foreign body (chili pepper skin). Symptoms resolved after removal of the foreign body (chili pepper skin) via electronic bronchoscopy.Results: Carcinoembryonic antigen levels were significantly elevated. Chest CT scan revealed a high-density nodular shadow and calcification foci in the right lower lobe. Ultrathin bronchoscopy demonstrated a bronchial foreign body (chili pepper skin) visible in the basal segment of the right lower lobe. Pathological examination of bronchial tissue from the right lower lobe showed no tumor cells.Conclusions: It is necessary to pay attention to the calcification of the trachea and the differential diagnosis of lung cancer, especially when the level of carcinoembryonic antigen is increased.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"2016-2019"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Progress on Extracellular Exosome Isolation and Enrichment Techniques. 细胞外泌体分离富集技术的研究进展。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250756

Lin Xu, Yu Tian

{"title":"Research Progress on Extracellular Exosome Isolation and Enrichment Techniques.","authors":"Lin Xu, Yu Tian","doi":"10.7754/Clin.Lab.2025.250756","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250756","url":null,"abstract":"Background: Exosomes are nanoscale vesicles that encapsulate a broad spectrum of bioactive molecules, including proteins, lipids, mRNA, miRNA, lncRNA, and DNA, and are present in various biological fluids, such as blood, saliva, and urine, where they serve as key mediators in intercellular communication and play critical roles in a wide range of physiological and pathological processes. This review aims to provide a comprehensive overview of current techniques for exosome isolation and enrichment, along with a detailed comparative analysis of their performance characteristics.Methods: Studies concerning exosome isolation and enrichment were systematically reviewed and organized based on methodological approaches, with a particular focus on the principles, procedures, and comparative features of each technique.Results: The principal isolation strategies comprise ultracentrifugation, polymer-based precipitation, filtration, size exclusion chromatography, immunoaffinity capture, and microfluidics-based techniques. Exosome enrichment approaches can be broadly categorized into physical methods (centrifugation, precipitation, filtration, and size exclusion chromatography) and biological methods (immunomagnetic bead-based technique).Conclusions: Exosomes hold considerable potential in the diagnosis, treatment, and prognostic evaluation of clinical diseases. Efficient isolation and enrichment are prerequisites for downstream analyses and applications. Advances in these methodologies are therefore critical for translating exosome research into effective diagnostic and therapeutic strategies.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"1841-1850"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Progress of Traumatic Brain Injury and Its Related Markers. 外伤性脑损伤及其相关标志物的研究进展

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250960

Yuqian Zhou, Xin Lu, Zhang Bu, Ke Wang, Peng Yang, Feng Xu

{"title":"Research Progress of Traumatic Brain Injury and Its Related Markers.","authors":"Yuqian Zhou, Xin Lu, Zhang Bu, Ke Wang, Peng Yang, Feng Xu","doi":"10.7754/Clin.Lab.2025.250960","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250960","url":null,"abstract":"Background: Traumatic brain injury (TBI) represents a leading global cause of mortality and disability, imposing substantial economic burdens on societies worldwide. Recent advances in molecular biology have identified several biomarkers with potential to improve TBI prognosis, yet these discoveries necessitate more comprehensive analysis and systematic summarization of their underlying mechanisms of action.Methods: This article systematically collates and reviews recent research on key molecular biomarkers in TBI, in-cluding AQP4, NF-κB, ICAM-1, GFAP, UCH-L1, NF-L, NGF, and tau protein. We have synthesized existing evidence and provide a narrative synthesis regarding these biomarkers' mechanisms of action, diagnostic accuracy, and prognostic utility in TBI.Results: The biomarkers summarized in this review play pivotal roles in the pathological processes of traumatic brain injury, including edema, inflammation, axonal injury, and neurodegeneration. Accumulating evidence indicates that these biomarkers demonstrate substantial potential to facilitate early diagnosis, enable accurate monitoring of disease progression, and improve assessment of long-term patient outcomes.Conclusions: Monitoring these biomarkers holds significant clinical importance, as it enables early clinical intervention, optimization of therapeutic strategies, and ultimately reduction of disability and mortality rates. The translation of these biomarkers into routine clinical practice represents a crucial step toward improving outcomes for TBI patients.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"1851-1857"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unexpected Identification of Anti-Diᵃ During Routine Pretransfusion Testing. 在常规输血前检测中意外发现抗di -。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.251012

Kuo-Tun Yen, Ying-Ju Chen, Tze-Kiong Er

{"title":"Unexpected Identification of Anti-Diᵃ During Routine Pretransfusion Testing.","authors":"Kuo-Tun Yen, Ying-Ju Chen, Tze-Kiong Er","doi":"10.7754/Clin.Lab.2025.251012","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.251012","url":null,"abstract":"Background: Anti-Diᵃ is a clinically significant alloantibody directed against a low-prevalence antigen in the Diego blood group system. While rare in most populations, its prevalence is notably higher among East Asian and indigenous South American groups. Detection is often challenging due to the limited presence of Dia (+) cells in routine antibody screening panels.Methods: We describe a case involving a 62-year-old male inpatient at Asia University Hospital who was evaluated for symptomatic anemia. Routine pretransfusion testing using an automated analyzer revealed a positive antibody screen with a sparse and non-congruent reaction pattern. Subsequent manual repeat testing and serologic workup with an 11-cell panel showed reactivity limited to Diᵃ-positive cells. The presence of anti-Diᵃ was confirmed by testing the patient's plasma against Diᵃ-positive red blood cells obtained from the local blood center. After confirming compatibility using the manual polybrene method, the patient received a safe transfusion of leukocyte-reduced red blood cells without adverse reactions.Results: This case underscores the importance of recognizing atypical reactivity patterns that may indicate a low-incidence antibody. It highlights the need for a rapid pathway to selected cell testing and reference support for accurate antibody identification. While Dia (-) units are readily available, appropriate LIS documentation, clear clinical communication, and staff awareness are essential to ensure safe and timely transfusion care.Conclusions: This case demonstrates the value of a structured workflow for identifying rare antibodies like anti-Diᵃ and reinforces the need for proactive laboratory protocols to safe and effective transfusion practice.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"2042-2045"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and Microbiological Characteristics of Bloodstream Infections due to CRKP. CRKP血流感染的临床和微生物学特征。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250860

Li-Li Fang, Xiao-Ying Lan, Zhi-Meng Li, Jian-Ping Sun, Hai-Yue Liu, Guo-Lin Hong, Xian-Ming Liang

{"title":"Clinical and Microbiological Characteristics of Bloodstream Infections due to CRKP.","authors":"Li-Li Fang, Xiao-Ying Lan, Zhi-Meng Li, Jian-Ping Sun, Hai-Yue Liu, Guo-Lin Hong, Xian-Ming Liang","doi":"10.7754/Clin.Lab.2025.250860","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250860","url":null,"abstract":"Background: Bloodstream infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) often result in high mortality rates. This study aimed to explore the local epidemiology and risk factors of CRKP bloodstream infections, characterize the resistance and virulence genes of CRKP isolates in this region of China, and identify the circulating hypervirulent Klebsiella pneumoniae strains locally.Methods: In a case-controlled retrospective study, we examined the prevalence, patient background, and risk factors for CRKP BSIs.Results: The overall CRKP BSI incidence was 2.2 (54/2,420,826) per 100,000 patient-days. Acute operation (p = 0.012), being a resident in a long-term-care facility (p = 0.019), transfer to ICU within 30 days (p = 0.014), pulmonary diseases (p = 0.022), renal diseases (p = 0.004), liver diseases (p = 0.008), abdominal infections (p = 0.005), gastric tube (p = 0.011), quinolone use (p = 0.029), carbapenem use (p = 0.001), and antifungal agent use prior to culture within 30 days (p = 0.001) were risk factors for CRKP BSIs. In total, 88.9% (48/54) isolates were detected with KPC carbapenemase, followed by NDM carbapenemase at 7.4% (4/54). YcfM, wabG, and entB were present in all strains. The following four virulence genes had a positivity rate of over 90%: fimH (98.15%), mrkD (96.30%), uge (92.60%), and kpn (92.60%).Conclusions: Acute operation, being a resident in a long-term-care facility, transfer to ICU within 30 days, pulmonary diseases, renal diseases, liver diseases, abdominal infections, gastric tube, quinolone use, carbapenem use, and antifungal agent use prior to culture within 30 days were risk factors for CRKP BSIs. KPC carbapenemase ranked first in isolates of CRKP BSIs. Virulence genes were widely present.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"1892-1900"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of a Fluorescence Immunoassay-Based Interferon-Gamma Release Assay for Latent Tuberculosis Infection. 基于荧光免疫分析的干扰素γ释放法检测潜伏结核感染的性能。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250937

Eungjun Yoon, Jae Joon Lee, Hyejin Ryu, Ajoy Kumar Verma, Ashish Ranjan, Lokender Kumar, Rajesh Kumar, Gavish Kumar, Ankita Dey, Tae Yeul Kim, Minjeong Nam

{"title":"Performance of a Fluorescence Immunoassay-Based Interferon-Gamma Release Assay for Latent Tuberculosis Infection.","authors":"Eungjun Yoon, Jae Joon Lee, Hyejin Ryu, Ajoy Kumar Verma, Ashish Ranjan, Lokender Kumar, Rajesh Kumar, Gavish Kumar, Ankita Dey, Tae Yeul Kim, Minjeong Nam","doi":"10.7754/Clin.Lab.2025.250937","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250937","url":null,"abstract":"Background: Latent tuberculosis infection (LTBI) diagnosis is crucial for controlling tuberculosis (TB). Interferon-gamma release assay (IGRA) diagnoses LTBI by measuring interferon-gamma produced by TB antigen-stimulated T-cells. In this study, we evaluated the clinical performance of AFIAS IGRA-TB (Boditech Med, Inc.).Methods: A total of 200 subjects, including those without known risk factors for TB infection, those with known TB contact, and those with active TB, were analyzed using both AFIAS IGRA-TB and QuantiFERON-TB Gold In-Tube (QFT-GIT; Qiagen) according to the manufacturers' instructions.Results: The positive, negative, and overall percent agreements between AFIAS IGRA-TB and QFT-GIT were 88.2% (95% confidence interval [CI]: 76.6 - 94.5%), 95.4% (95% CI: 90.4 - 97.9%), and 93.4% (95% CI: 88.8 - 96.2%), respectively. Cohen's kappa coefficient was 0.84, indicating excellent agreement.Conclusions: AFIAS IGRA-TB demonstrated equivalent clinical performance to QFT-GIT. Given its ease of use, minimal reliance on trained personnel or complex equipment, and rapid turnaround time, AFIAS IGRA-TB has the potential to serve as an alternative test for diagnosing LTBI and active TB in resource-limited settings.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"1919-1924"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Value of the VITEK 2 System in Routine Laboratory Identification of Non-Candida albicans Yeasts. VITEK 2系统在非白色念珠菌实验室常规鉴定中的应用价值。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250953

Gonca Erkose-Genc, Berkay Yuksel, Ilvana Caklovica-Kucukkaya, Zayre Erturan

{"title":"The Value of the VITEK 2 System in Routine Laboratory Identification of Non-Candida albicans Yeasts.","authors":"Gonca Erkose-Genc, Berkay Yuksel, Ilvana Caklovica-Kucukkaya, Zayre Erturan","doi":"10.7754/Clin.Lab.2025.250953","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250953","url":null,"abstract":"Background: As fungal infections represent a growing global health concern, particularly among vulnerable populations, and antifungal susceptibility may vary even within the same genus or species complex, accurate and rapid diagnostic tools are increasingly essential. VITEK 2 is an automated identification system based on biochemical methods and widely used in microbiology laboratories, especially in developing countries. This study aimed to retrospectively evaluate the performance and accuracy of the VITEK 2 system in identifying yeasts, particularly rare and emerging species such as Candida auris.Methods: Data of a total of 173 yeast isolates obtained from clinical specimens were evaluated, including 96 common non-albicans Candida species, 68 rare Candida species, and nine non-Candida yeasts.Results: Overall, 111 (64.2%) isolates were accurately identified, eight (4.6%) were misidentified, and 54 (31.2%) were not identified. Accurate identification rates for common, rare, and non-Candida species were 73.9% (71/96), 57.3% (39/68), and 11.1% (1/9), respectively. Among 44 C. auris isolates, 36 (81.8%) were accurately identified, while three (6.8%) were misidentified (two isolates as Cryptococcus laurentii and one isolate as Candida duobushaemulonii), and five (11.4%) remained unidentified.Conclusions: In conclusion, more studies evaluating the latest versions of the VITEK 2 system, with a greater number of isolates, particularly rare yeast species, are needed.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"1941-1947"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Disk Diffusion and Agar Dilution for Neisseria gonorrhoeae Susceptibility Testing and Update. 涂片扩散法与琼脂稀释法淋病奈瑟菌药敏试验的比较与更新。

IF 0.7 4区医学

Clinical laboratory Pub Date : 2026-09-01 DOI: 10.7754/Clin.Lab.2025.250757

Shudong Tan, Tianji Qu, Jing Ai, Yaoyang Fu

{"title":"Comparison of Disk Diffusion and Agar Dilution for Neisseria gonorrhoeae Susceptibility Testing and Update.","authors":"Shudong Tan, Tianji Qu, Jing Ai, Yaoyang Fu","doi":"10.7754/Clin.Lab.2025.250757","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250757","url":null,"abstract":"Background: The aim of this study was to evaluate the validity of the disk diffusion for antimicrobial susceptibility testing of clinical Neisseria gonorrhoeae isolates, using the agar dilution as the reference standard. Four antimicrobial agents were assessed: penicillin, ciprofloxacin, ceftriaxone, and spectinomycin, with updated susceptibility data provided.Methods: A total of 185 Neisseria gonorrhoeae isolates, identified by 16S rRNA sequence analysis, were collected from clinical specimens obtained from patients in four general hospitals in Changsha, China, between 2014 and 2018. Antimicrobial susceptibility to the four agents was determined by both disk diffusion and agar dilution. Correlation and agreement between the two methods were analyzed by plotting inhibition zone diameters against their corresponding minimal inhibitory concentrations (MICs) and performing linear regression analysis.Results: By disk diffusion, the susceptibility rates were 1.1% (penicillin), 0.6% (ciprofloxacin), 98.4% (ceftriaxone), and 100% (spectinomycin), while by agar dilution the rates were 1.6% (penicillin), 0% (ciprofloxacin), 95.7% (ceftriaxone), and 100% (spectinomycin). The disk diffusion showed high categorical agreement with the agar dilution, with concordance rates of 98.9%, 99.4%, 96.2%, and 100% for penicillin, ciprofloxacin, ceftriaxone, and spectinomycin, respectively. Pearson correlation coefficients were -0.78, -0.65, -0.47, and -0.61 (all p < 0.001). Although discrepancies between the two methods were acceptable, further analysis is required to clarify discordant results observed for ceftriaxone in some isolates.Conclusions: The disk diffusion remains a reliable qualitative approach for antimicrobial susceptibility testing of Neisseria gonorrhoeae, with results largely comparable to the agar dilution reference method.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 9","pages":"1876-1881"},"PeriodicalIF":0.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0