Clinical laboratory最新文献

{"title":"Case Analysis of a Repeated Hydrops Fetalis Caused by Irregular Antibodies of Anti-E and Anti-c.","authors":"Haijuan Wang, Jian Chen, Guojin Ou","doi":"10.7754/Clin.Lab.2025.250103","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250103","url":null,"abstract":"<p><strong>Background: </strong>Blood group irregular antibody is a major factor that may cause adverse pregnancy outcomes. We aimed to emphasize the importance of prenatal blood antibody screening and popularize antenatal care knowledge by sharing a case of hydrops fetalis caused by anti-E and anti-c alloimmunization antibodies.</p><p><strong>Methods: </strong>We retrospectively analyzed the clinical and laboratory data of a pregnant woman with repeated fetal edema caused by anti-E and anti-c irregular antibodies. Irregular antibody screening, antibody identification, and antibody titer detection were performed.</p><p><strong>Results: </strong>A pregnant woman aged 30 years old, in whom hydrops fetalis was found by color Doppler ultrasound at 32 + 5 gestation weeks. Irregular antibody screening was positive and anti-E and anti-c were detected in plasma. The results were negative by saline method. Antibody titers of anti-E and anti-c were 1,024 and 128, respectively, and Rh blood group was CCDee (R1R1). Eventually, a fatal termination had to be chosen for serious hydrops fetalis.</p><p><strong>Conclusions: </strong>Hydrops fetalis, stillborn fetus, and kernicterus are the severest outcomes of hemolytic disease of the fetus and newborn (HDFN). Anti-E and anti-c are common alloantibodies that lead to adverse fetal outcomes. Such adverse pregnancy outcomes can be minimized by improving pregnant women's awareness of HDFN and reinforcing the management of irregular blood group antibodies during pregnancy.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil/Lymphocyte Ratio and Monocyte/Lymphocyte Ratio Concerning Renal Injury in Patients with Lupus Nephritis.","authors":"Dantong Wu, Yao Wang, Xiao Han, Haiyan Gao, Yue Zhang, Hongjun Lou","doi":"10.7754/Clin.Lab.2025.241217","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241217","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to investigate the correlation between the neutrophil/lymphocyte ratio (NLR), monocyte/lymphocyte ratio (MLR), and platelet/lymphocyte ratio (PLR) with renal injury in patients with lupus nephritis (LN).</p><p><strong>Methods: </strong>Data was obtained from 26 patients with LN and 72 with systemic lupus erythematosus (SLE) who received treatment between January 2021 and October 2022 at the First Hospital of Heilongjiang University of Traditional Chinese Medicine. Venous blood was collected from the patients. We determined blood urea, creatinine (Cr), cystatin C (CYSC), and uric acid (UA) and calculated the NLR, MLR, and PLR.</p><p><strong>Results: </strong>The NLR and MLR were significantly greater in the LN group compared to SLE group (p < 0.05). Spearman's correlation analysis demonstrated that MLR had a positive correlation with UA (p < 0.05), while PLR ex-hibited a positive correlation with Cr and UA (p < 0.05). The NLR and MLR p-values were less than 0.05, and the respective areas under the receiver operating characteristic (ROC) curves for each were 0.761 and 0.720.</p><p><strong>Conclusions: </strong>NLR and MLR are strongly related to renal injury in patients with LN. These ratios demonstrate a reasonable correlation with the commonly used markers of renal injury and have significant clinical value in evaluating impaired renal function in such patients.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Misinterpretable Band on Urine Protein Electrophoresis in Hematuric Patients.","authors":"Sang Mook Kim, Jae Hee Lee, Ji Yeon Ham, Nan Young Lee, Kyung Eun Song","doi":"10.7754/Clin.Lab.2025.250121","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250121","url":null,"abstract":"<p><strong>Background: </strong>Urine protein electrophoresis (PEP) is widely employed to detect proteinuria and monoclonal protein (M protein), which is important for diagnosing and monitoring multiple myeloma. However, the appearance of unusual bands can confound the interpretation.</p><p><strong>Methods: </strong>We described two clinical cases in which a distinct beta-region band in urine PEP associated with hematuria. To investigate this phenomenon, we conducted an experiment by adding both lysed and non-lysed red blood cells (RBCs) to normal urine specimens and then performed urine PEP.</p><p><strong>Results: </strong>In both of our cases, the beta-region band disappeared after hematuria improved. In the experimental setup, the group with RBC lysis at counts above 500/µL produced a marked band in the beta region without gross hematuria, whereas the group with non-lysed RBCs required much higher concentrations to generate faint bands.</p><p><strong>Conclusions: </strong>Our findings demonstrate that hematuria, particularly when red blood cells undergo lysis, can produce a distinct band in the beta region on urine PEP that may be misinterpreted as a monoclonal protein. Careful correlation with urinalysis results is therefore crucial to prevent misdiagnosis, especially in patients with microscopic hematuria.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frailty Assessment and NK Cell Function in Multiple Myeloma: a Comprehensive Analysis.","authors":"QiuLing Yao, Zhe Su, Lan Luo, HongQuan Luo","doi":"10.7754/Clin.Lab.2024.241121","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241121","url":null,"abstract":"<p><strong>Background: </strong>Frailty is a clinical syndrome characterized by decreased muscle strength and endurance, as well as deterioration of multiple system functions and metabolic status. Frailty is particularly common in patients with multiple myeloma (MM), which seriously affects their quality of life.</p><p><strong>Methods: </strong>From January through November 2023, Sichuan Provincial People's Hospital recruited 21 MM patients and 16 healthy individuals and used advanced ten-color flow cytometry to detect the expression levels of NK cell-related markers in both groups and explore their changes in the revised international staging system (R-ISS) stages and different frailty scores.</p><p><strong>Results: </strong>The total number of NK cells in the patient group was significantly higher than that in the control group, but its function was impaired. As the MM disease progressed and the degree of frailty increased, the proportion of activated receptors on NK cells in the patient group decreased. In addition, there were differences in the distribution of NK cell-related markers in patients under different frailty assessment systems.</p><p><strong>Conclusions: </strong>The total number and subpopulations of NK cells in MM patients have changed, and the expression of natural killer receptors, CD244, CD16, and HLA-DR significantly decreased with the progression of frailty. These changes can help to understand the overall health status and immune state of patients and help physicians develop personalized treatment strategies, improve outcomes, and enhance the quality of life for patients.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anemia Among Women and Children in Saudi Arabia: Is it a Public Health Burden?","authors":"Hassan A Hamali","doi":"10.7754/Clin.Lab.2025.250102","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250102","url":null,"abstract":"<p><strong>Background: </strong>Anemia is a global health burden affecting developing and developed countries alike, estimated to affect one-third of the world's population. The most prevalent type of anemia globally is iron deficiency anemia. The groups most vulnerable to developing anemia include children, women (particularly pregnant women), and the elderly, primarily in developing nations. Anemia is also widespread in Gulf countries, including Saudi Arabia, where estimates suggest the disease affects up to 70% of women and children. The aim of the current review was to report the incidence of anemia among women and children in Saudi Arabia and the impact of anemia on pregnancy.</p><p><strong>Methods: </strong>The main electronic databases were searched for publications on anemia among women and children in Saudi Arabia.</p><p><strong>Results: </strong>In Saudi Arabia, the incidence of anemia among children and women ranges from 12.5% to 70%, varying by province. Younger pregnant women exhibit a higher prevalence of anemia than older pregnant women. The primary risk factors for developing anemia among children and women in Saudi Arabia include lifestyle choices, low dietary intake, the number of pregnancies, and sociodemographic factors. Consequently, anemia is a major health burden in Saudi Arabia, necessitating serious planning and intervention to reduce anemia-related complications among women and children.</p><p><strong>Conclusions: </strong>Despite substantial development and improvements in socio-economic factors, anemia remains a major health issue for children and women in Saudi Arabia. Therefore, the underlying factors, including nutritional and other risk factors, warrant further investigation.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Iodine Status in Adults and Establishment of Reference Ranges for Urine Iodine Concentration in Turkey.","authors":"Hande Karpuzoglu, Canan Kucukgergin, Abdurrahman F Aydin","doi":"10.7754/Clin.Lab.2025.240758","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.240758","url":null,"abstract":"<p><strong>Background: </strong>Iodine is essential for thyroid hormone production and is critical for growth, development, and reproductive health. It also acts as an antioxidant and influences cell differentiation. Iodine deficiency can lead to hypothyroidism. Urinary iodine concentration (UIC), often assessed through spot samples for convenience, is a valuable indicator of iodine intake. The median UIC of spot samples is commonly used to categorize a population's iodine status. This study aimed to assess the prevalence of iodine deficiency and establish reference ranges for iodine levels in adults aged 18 and above in Turkey.</p><p><strong>Methods: </strong>Spot urinary iodine concentrations were measured at Istanbul Medical Faculty between July 2018 and July 2023. Iodine measurements were conducted using a modified microplate method based on the Sandell-Kolthoff reaction. The reference ranges for the urine iodine test were calculated using the Hoffman method.</p><p><strong>Results: </strong>The iodine levels varied in this study: 13% had < 50 µg/L, 24% had 50 - 99 µg/L, 25% had 100 - 149 µg/L, 16% had 150 - 199 µg/L, 12% had 200 - 299 µg/L, and 10% had > 300 µg/L. The median UIC for Turkish adults was 122 µg/L, considered optimal by WHO standards. The iodine reference range for 18- to 80-year-olds was 20 - 330 µg/L, with a 116 µg/L median. There was no significant correlation between iodine levels and thyroid hor-mones or antibodies.</p><p><strong>Conclusions: </strong>The results of this study indicate that iodine levels among adults in the Turkish population from 2018 to 2023 are sufficient. Furthermore, the established reference intervals will facilitate the effective monitoring of iodine status by health professionals in clinical and laboratory settings.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota, Disorders of Gut-Brain Interaction and Psychiatric Disorders: a Mendelian Randomization Study.","authors":"Zhenni Wang, Haiyan Wang, Ning Wang, Xiaohui Qu, Xiaofeng Mu, Shaoyun Cheng","doi":"10.7754/Clin.Lab.2025.250107","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250107","url":null,"abstract":"<p><strong>Background: </strong>Observational studies suggest that there are associations among gut microbiota, disorders of gut-brain interaction (DGBIs) and psychiatric disorders. Therefore, the aim of this study was to use Mendelian randomization (MR) to systematically identify the causality of the associations among the abundances of several gut microbiota and the risk of developing DGBIs and psychiatric disorders.</p><p><strong>Methods: </strong>Genetic data associated with gut microbiota, DGBIs, and psychiatric disorders were obtained from large-scale genome-wide association studies (GWASs). Inverse-variance weighting, MR-Egger, and weighted median methods were used to examine causal associations. The sensitivity analyses were conducted via the MR-Egger intercept test, Cochran's Q test, MR-pleiotropy residual sum and outlier (MR-PRESSO) test, leave-one-out analysis, and funnel plots. Reverse-MR analysis was performed to evaluate the possibility of reverse causation. Finally, we used MR mediation analysis to explore potential mediators of the causal associations among the abundances of gut microbiota and the risk of developing DGBIs and psychiatric disorders.</p><p><strong>Results: </strong>Our MR analysis revealed 44 causal relationships between the abundances of several gut microbiota and the risk of developing DGBIs and 66 causal relationships between the abundances of several gut microbiota and the risk of developing psychiatric disorders. In addition, in the reverse-MR analysis, 15 causal relationships between the risk of developing DGBIs and the abundances of several gut microbiota and 47 causal relationships between the risk of developing psychiatric disorders and the abundances of several gut microbiota were explored. Our results showed that the abundances of some microbiota and their child taxa might be closely associated with the risk of developing certain diseases. Moreover, we observed one causal relationship between the risk of developing DGBIs and the risk of developing psychiatric disorders and 7 causal relationships between the risk of developing psychiatric disorders and the risk of developing DGBIs. Compared with the causal effect of the risk of developing DGBIs on the risk of developing psychiatric disorders, the risk of developing psychiatric disorders was more likely to causally influence the risk of developing DGBIs. Further sensitivity analyses reinforced the robustness of these results.</p><p><strong>Conclusions: </strong>Our results indicate potential genetic predispositions linking gut microbiota, DGBIs, and psychiatric disorders. This information may be useful for providing new insights into the underlying pathophysiological modulators and treatment strategies for bidirectional dysregulation of brain-gut interactions.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and Clinical Significance of PIM1 in Newly Diagnosed Patients with Acute Myeloid Leukemia.","authors":"Zhong-Yu Li, Yuan-Yuan Tan, Meng-Meng Zhang, Gui-Ling Liu, Wei Yao, Gui-Zhen Su, Wan-Wan Wang, Qiang Li, Jia-Jia Li","doi":"10.7754/Clin.Lab.2024.241213","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241213","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the expression level of proviral integration of Moloney murine leukemia virus 1 (PIM1) in acute myeloid leukemia (AML) and to investigate its clinical significance, including its expression and prognostic value in AML.</p><p><strong>Methods: </strong>Bone marrow samples were obtained from 80 newly diagnosed AML patients (observation group) and 20 healthy individuals (control group). Clinical and pathological characteristics of AML patients were collected. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure PIM1 expression levels in the two groups. The association between PIM1 expression and clinicopathological characteristics and prognosis in AML patients was analyzed. Kaplan-Meier survival curves were used to assess the impact of PIM1 expression on overall survival (OS), while Cox proportional hazards regression was employed to identify prognostic factors in AML patients.</p><p><strong>Results: </strong>PIM1 expression was significantly higher in AML patients compared to the control group (p < 0.0001). Within the AML cohort, the high PIM1 expression group showed significant differences in gender distribution, FAB classification, treatment response, and prognosis compared to the low PIM1 expression group (p < 0.05). The OS of patients with high PIM1 expression was markedly shorter than that of patients with low PIM1 expression (p < 0.05). Furthermore, high PIM1 expression was identified as an independent risk factor for poor prognosis in AML patients.</p><p><strong>Conclusions: </strong>PIM1 is overexpressed in AML patients and is associated with adverse clinicopathological characteristics and poor prognosis. PIM1 may serve as a prognostic biomarker and a potential therapeutic target in AML.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-Cancer Bioinformatics Analysis of Matrix Metalloproteinase-9 (MMP9).","authors":"Qianmei Zhan, Mengqiu Lan, Wenjie Huang, Yinxiu Chi, Dongliang Zhang","doi":"10.7754/Clin.Lab.2025.241132","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241132","url":null,"abstract":"<p><strong>Background: </strong>Matrix metalloproteinase-9 (MMP9) is a member of the matrix metalloproteinase family of proteases that have been linked to several major normal and pathologic processes. Although cell-based, animal-based, or clinical-based evidence supports the relationship between MMP9 and cancers, the role of MMP9 in pan-cancer is still not fully understood.</p><p><strong>Methods: </strong>We explored the prognostic value and potential immunological roles of MMP9 in 33 cancer types using the Cancer Genome Atlas datasets.</p><p><strong>Results: </strong>MMP9 is highly expressed at the RNA level in most cancers but is present at a low protein level in breast cancer, liver cancer, lung adenocarcinoma, and ovarian cancer. Furthermore, there are distinct associations be-tween MMP9 expression and tumor patient prognosis. MMP9 expression was found to be associated with tumor mutational burden and microsatellite instability in eight cancer types, whereas it was associated with DNA methylation at multiple probes in 31 cancer types. Additionally, MMP9 expression was positively associated with the infiltration levels of most immune cells in 33 cancer types. Moreover, extracellular matrix organizing-related and collagen metabolism-related functions are involved in the functional mechanism of MMP9.</p><p><strong>Conclusions: </strong>Our pancancer study offers a relatively comprehensive understanding of the role of MMP9 in tumorigenesis and tumor immunity in different tumors.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of Prevalence of Respiratory Syncytial Virus: Accounting for False Negative.","authors":"Pathum Sookaromdee, Viroj Wiwanitkit","doi":"10.7754/Clin.Lab.2025.241252","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241252","url":null,"abstract":"","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}