Clinical laboratory最新文献_第3页

Vitamin D Reduces Blood Glucose Levels and HbA1c by Inducing Hepatic GLUT2 and Muscular GLUT4 in Type 1 Diabetic Mice. 维生素D通过诱导1型糖尿病小鼠肝脏GLUT2和肌肉GLUT4降低血糖水平和HbA1c

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250111

Ayman S Alhazmi

{"title":"Vitamin D Reduces Blood Glucose Levels and HbA1c by Inducing Hepatic GLUT2 and Muscular GLUT4 in Type 1 Diabetic Mice.","authors":"Ayman S Alhazmi","doi":"10.7754/Clin.Lab.2025.250111","DOIUrl":"10.7754/Clin.Lab.2025.250111","url":null,"abstract":"Background: Diabetes mellitus is still a major health problem affecting individuals all over the world. Type 1 diabetes mellitus occurs due to insulin deficiency resulting from the destruction of pancreatic β-cells. This study aimed to investigate how vitamin D reduces blood glucose levels and HbA1c.Methods: The present study consists of 30 Balb/c mice classified into control, untreated diabetic mice, and diabetic mice treated with vitamin D.Results: Blood glucose levels, HbA1c, and lipid profiles were decreased in diabetic mice treated with vitamin D supplementation. Plasma and hepatic GLUT2 were increased in diabetic mice treated with vitamin D compared with the untreated diabetic group. Furthermore, vitamin D supplementation induces plasma and muscular GLUT4.Conclusions: The present study concluded that vitamin D supplementation induces hepatic GLUT2 and muscular GLUT4 in type 1 diabetic mice. Induction of hepatic GLUT2 and muscular GLUT4 induces glucose uptake by the liver and skeletal muscle, respectively, then reduces blood glucose levels. This mechanism may be the way by which vitamin D reduces blood glucose levels and HbA1c in type 1 diabetes mice.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary Acute Myeloid Leukemia with Concomitant BCR∷ABL and NPM1 Mutation. 原发性急性髓系白血病合并BCR∷ABL和NPM1突变。

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.241212

Jiayu Xu, Fuhua Zhang, Jiabao Liang

{"title":"Primary Acute Myeloid Leukemia with Concomitant BCR∷ABL and NPM1 Mutation.","authors":"Jiayu Xu, Fuhua Zhang, Jiabao Liang","doi":"10.7754/Clin.Lab.2025.241212","DOIUrl":"10.7754/Clin.Lab.2025.241212","url":null,"abstract":"Background: This study aims to gain further insights into the characteristics of the rare subtype of acute myeloid leukemia (AML) with BCR∷ABL by analyzing laboratory detection results of various gene mutations, such as NPM1.Methods: Laboratory detection results of multiple gene missense mutations, including NPM1, were analyzed in a case of primary AML with BCR∷ABL.Results: The patient exhibited morphological features of acute leukemia in the bone marrow. Immunophenotyping of bone marrow blast cells revealed the presence of CD33+, CD123+, CD13+, CD38+, CD11b+ (partial cells), CD7+ (partial cells), and CD56+ (partial cells) markers. Chromosomal karyotyping analysis showed the presence of the BCR-ABL fusion gene formed by t(9;22). BCR∷ABL genotyping and quantitative detection indicated a BCR∷ABL-P230/ABL ratio of 9.723%. Gene screening revealed the positivity of NMP1 and missense mutations in NOTCH2, TET1, CDKN2A, KMT2C, and USH2A genes. Combining these results with previous laboratory tests, the diagnosis of AML with BCR∷ABL subtype and concurrent NPM1-positive status was confirmed. The patient underwent treatment with dasatinib, venetoclax, azacitidine chemotherapy, and anti-infection therapy. The patient's vital signs remained stable overall, and the patient was followed up after discharge.Conclusions: AML with BCR∷ABL cases are relatively rare, and the establishment of standardized diagnostic and treatment strategies is still lacking. Our research findings emphasize the importance of including BCR∷ABL detection in the diagnostic examination of AML to enable the provision of the most appropriate risk classification and treatment options for patients.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Hidden Pitfall: Prozone Effect in Serum Free Light Chain with Nephelometry Analysis. 一个隐藏的陷阱：用浊度法分析血清游离轻链中的丙氨酸效应。

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250217

Syed B Hashmi, Imran Siddiqui, Samia Khan, Sibtain Ahmed

引用次数: 0

A Rare Case of Kikuchi-Fujimoto Disease Complicated with Hemophagocytic Lymphohistiocytosis. 罕见的菊池-藤本病合并噬血细胞性淋巴组织细胞增多症1例。

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250227

Yingying Sun, Chengcheng Wu, Chunyan Liu, Zonghong Shao

引用次数: 0

Reference Intervals for SII, NLR, PLR, and LMR: a Comparison of Nonparametric and refineR Approaches Using Big Data. SII， NLR， PLR和LMR的参考区间：使用大数据的非参数和精化方法的比较。

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250104

Mehmet F Alpdemir, Medine Alpdemir, Esra F Oğuz, Funda Eren

{"title":"Reference Intervals for SII, NLR, PLR, and LMR: a Comparison of Nonparametric and refineR Approaches Using Big Data.","authors":"Mehmet F Alpdemir, Medine Alpdemir, Esra F Oğuz, Funda Eren","doi":"10.7754/Clin.Lab.2025.250104","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250104","url":null,"abstract":"Background: Based on the widespread use of the systemic immune-inflammation index (SII), neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), and lymphocyte-monocyte ratio (LMR), markers, we aimed to calculate and compare the reference intervals (RIs) of these indices in adults, using both nonparametric method according to the Clinical and Laboratory Standards Institute's (CLSI) EP28-A3C:2010 guideline and refineR algorithm using a large dataset.Methods: We analyzed data from 293,585 adults (18 - 65 years) retrospectively obtained from complete blood count results (using laboratory information system). The study involved a two-stage outlier exclusion process. White blood cells, platelets, neutrophils, lymphocytes, and monocytes were measured using the Advia 2120i (Siemens Healthineers, Germany), and SII, NLR, PLR, and LMR were calculated. Subgroups were formed based on age and gender, and RIs were estimated using an indirect sampling technique, specifically the nonparametric statistical method (2.5th and 97.5th percentiles) and refineR algorithm.Results: Our study showed that the values of SII, NLR, PLR, and LMR varied significantly between males and females. For all the parameters, the lower and upper limits of reference intervals (RIs) for males were higher when using the RefineR algorithm. RIs based on age groups using CLSI guideline and the refineR algorithm were calculated, showing wider limits of the RIs in refineR.Conclusions: We detected a difference between the RI values of the indexes according to the different RI calculation methods and observed that the reference intervals calculated according to CLSI guideline have a narrower range than by refineR algorithm.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of NR1D1 in Colorectal Cancer: Impact on Prognosis, Immune Microenvironment, and Oncogenic Pathways. NR1D1在结直肠癌中的作用：对预后、免疫微环境和致癌途径的影响

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250221

Xingshu Zhu, Ming Wang, Deren Du, Xueyi Feng, Zhilong Huang, Jun Zhu, Congliang Rao

{"title":"Role of NR1D1 in Colorectal Cancer: Impact on Prognosis, Immune Microenvironment, and Oncogenic Pathways.","authors":"Xingshu Zhu, Ming Wang, Deren Du, Xueyi Feng, Zhilong Huang, Jun Zhu, Congliang Rao","doi":"10.7754/Clin.Lab.2025.250221","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250221","url":null,"abstract":"Background: The goal was to explore the impact of the NR1D1 gene on the occurrence, development, and prognosis of colorectal cancer (CRC) using bioinformatics approaches.Methods: CRC transcriptomic and clinical data from TCGA were analyzed to compare NR1D1 expression in tumors and various clinical stages. Survival differences between high and low NR1D1 expression groups were assessed using the R survival package. Univariate and multivariate Cox regression analyses identified independent prognostic factors, and a prognostic nomogram was constructed and evaluated via ROC and calibration curves. Differentially expressed genes were identified using the limma package, and functional enrichment was performed with clusterProfiler. The XCELL algorithm was used to evaluate differences in immune infiltration. Validation was conducted using GEO, HPA, qRT-PCR, and western blotting.Results: NR1D1 is overexpressed in CRC and correlates with advanced clinical stages. High NR1D1 expression is associated with poor prognosis, with multivariate Cox regression identifying NR1D1, age, stage, and NM grade as independent prognostic factors. The constructed model showed good performance (AUC > 0.70) at 1, 3, and 5 years. Enrichment analysis revealed NR1D1-related genes enriched in pathways like systemic lupus erythematosus and neutrophil extracellular trap formation. The low NR1D1 expression group showed increased immune infiltration, higher immune scores, and elevated immune microenvironment scores. NR1D1 negatively correlated with immune checkpoints, with significant differences in gene expression between high and low NR1D1 samples. High NR1D1 expression in CRC was confirmed in the GSE39582 dataset and HPA database, correlating with poor prognosis and validated by qRT-PCR and western blotting.Conclusions: NR1D1 may play a crucial oncogenic role in the occurrence and development of CRC.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic Value of Molecular and Clinical Features in Patients with NPM1 Gene Mutation in Acute Myeloid Leukemia. 急性髓系白血病患者NPM1基因突变的分子及临床特征对预后的影响

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2022.250132

Xiaoyu Li, Kaiqiang Xi

{"title":"Prognostic Value of Molecular and Clinical Features in Patients with NPM1 Gene Mutation in Acute Myeloid Leukemia.","authors":"Xiaoyu Li, Kaiqiang Xi","doi":"10.7754/Clin.Lab.2022.250132","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2022.250132","url":null,"abstract":"Background: Nucleophosmin 1 (NPM1) mutations represent one of the most frequent genetic alterations in acute myeloid leukemia (AML). However, the prognostic significance of concurrent molecular abnormalities and clinical features in NPM1-mutated AML remains to be fully elucidated.Methods: We retrospectively analyzed 73 adult AML patients with NPM1 mutations. Clinical characteristics, molecular features, and treatment outcomes were evaluated. Comprehensive molecular profiling was performed to detect concurrent mutations. Survival analysis included recurrence-free survival (RFS) and overall survival (OS), with multivariate analysis to identify independent prognostic factors.Results: The cohort showed a female predominance (54.8%), with a median age of 53.1 years. FLT3-ITD mutations were detected in 49.3% of patients, followed by DNMT3A (42.5%) and IDH1 (23.3%) mutations. Significant differences across FAB subtypes were observed in blast percentage (p = 0.003), WBC count (p = 0.013), and platelet count (p = 0.004). Multivariate analysis identified elevated WBC count (HR = 1.01, p = 0.008), increased LDH levels (HR = 1.0, p = 0.006), and FLT3-ITD mutations (HR = 2.55, p = 0.007) as independent adverse factors for RFS. For OS, low albumin levels (HR = 0.89, p = 0.017), DNMT3A mutations (HR = 2.49, p = 0.042), and treatment response were significant prognostic factors, while allogeneic hematopoietic stem cell transplantation showed a protective effect (HR = 0.08, p = 0.015).Conclusions: Our findings demonstrate that the clinical outcomes of NPM1-mutated AML are significantly influenced by concurrent molecular mutations and clinical parameters. The presence of FLT3-ITD and DNMT3A mutations, along with specific clinical features, identifies patients with adverse prognosis who might benefit from more intensive therapeutic strategies, including allogeneic transplantation.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Correlation between ABO Blood Types and Alcoholic Liver Disease. ABO血型与酒精性肝病的相关性

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250202

L N Wang, H X Yang, M H Dong, P P Liu, H Y Zhao, J Li

{"title":"The Correlation between ABO Blood Types and Alcoholic Liver Disease.","authors":"L N Wang, H X Yang, M H Dong, P P Liu, H Y Zhao, J Li","doi":"10.7754/Clin.Lab.2025.250202","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250202","url":null,"abstract":"Background: Since the discovery of ABO blood groups, there has been mounting evidence of the association between blood groups and diseases. However, so far, there is rarely available research about the potential role of ABO blood groups in alcohol liver disease (ALD). This study's aim was to investigate the relationship between ABO blood groups and the development of ALD in Qingdao, China.Methods: This experiment carried out a retrospective study enrolling 252 ALD patients as the case group and 373 healthy subjects as the control group. ABO blood group distribution was analyzed using the chi-squared test and logistic regression analysis.Results: Results showed that the distribution of ABO blood types between the two groups was significantly different (χ2 = 22.350, p < 0.05). Blood type O was less frequently observed [odds ratio (OR), 0.498; confidence interval (CI), 0.329 - 0.755; p < 0.05], while blood type AB was more frequently observed in the case group (OR, 2.550; CI, 1.589 - 4.092; p < 0.05).Conclusions: The findings indicate that populations with blood type AB might be more prone to ALD, whereas those with blood type O might be less susceptible to ALD, which will help in the risk stratification in ALD control.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Different Cases of Abnormal Hemoglobin A1c. 两例不同的糖化血红蛋白异常。

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250201

Jiehua Han, Yinjia Lou

{"title":"Two Different Cases of Abnormal Hemoglobin A1c.","authors":"Jiehua Han, Yinjia Lou","doi":"10.7754/Clin.Lab.2025.250201","DOIUrl":"10.7754/Clin.Lab.2025.250201","url":null,"abstract":"Background: Hemoglobin A1c (HbA1c) is a stable compound, which is an important indicator for diagnosing glycemia, evaluating blood glucose control in patients with diabetes, and guiding the formulation of treatment plans. However, the detection of HbA1c is easily affected by many factors, resulting in a false increase or decrease, which affects the accuracy of test results.Methods: In this paper, two abnormal HbA1c results were detected in the laboratory, which did not match the blood glucose detected at the same time. We adopted different detection systems to detect and proved that interference by different factors was observed in the results of glycosylated hemoglobin detection.Results: When the HbA1c results are abnormal or inconsistent with clinical symptoms, the inspection staff should find the problem in time and retest with different detection systems to confirm results or obtain the corrected results.Conclusions: HbA1c has been included in the diagnostic criteria of diabetes, as a routine test in laboratory. The laboratory staff should understand the characteristics and influencing factors of the HbA1c detection method in our laboratory, carefully observe the HbA1c map and query the patient case data, and find and solve problems, so as to provide more accurate reports for the clinic and better serve the clinic.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiepileptic Drugs and Influenza A Infection Complicated with Severe Thrombocytopenia. 抗癫痫药物与甲型流感感染并发严重血小板减少症。

IF 0.6 4区医学

Clinical laboratory Pub Date : 2025-09-01 DOI: 10.7754/Clin.Lab.2025.250233

Shanmei Lv, Hongkun Xv

{"title":"Antiepileptic Drugs and Influenza A Infection Complicated with Severe Thrombocytopenia.","authors":"Shanmei Lv, Hongkun Xv","doi":"10.7754/Clin.Lab.2025.250233","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250233","url":null,"abstract":"Background: Patients with epilepsy often require long-term antiepileptic medications, which can affect hematological parameters. Influenza (H1N1) infection is known to potentially cause thrombocytopenia. This case examines the clinical implications of a 29-year-old female patient with epilepsy who developed influenza and significant platelet reduction.Methods: A 29-year-old female patient with a history of epilepsy presented with fever lasting three days. She was undergoing treatment with multiple antiepileptic drugs, including levetiracetam, clonazepam, and carbamazepine. Comprehensive laboratory tests were performed to assess her complete blood count, liver and kidney function and to confirm influenza infection.Results: The patient displayed symptoms of fever, cough, and fatigue. Laboratory tests revealed a platelet count of 28.0 x 109/L, indicating severe thrombocytopenia. Influenza A (H1N1) was confirmed via PCR testing. The patient was started on antiviral therapy and supportive care. During the treatment, the patient's fever decreased and her general condition improved. A week later, her platelet count was back to its previous level, while still low.Conclusions: This case emphasizes the importance of regular platelet monitoring for patients on long-term antiepileptic drugs, particularly during H1N1 virus infections.","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0