Clinical laboratory最新文献

{"title":"Exploring the Effect of Malaria on Biochemical Parameters Among Sudanese Patients with Complicated Falciparum Malaria.","authors":"Mohamed O Ali, Abdelhakam G Tamomh, Nouraldein A Abdulrahman, Nazik S Ibrahim, Mudawi A Eltayeb, FadlAlla I Osman","doi":"10.7754/Clin.Lab.2025.250208","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250208","url":null,"abstract":"<p><strong>Background: </strong>Malaria is one of the most infectious diseases, and electrolyte imbalance and mineral disturbances are common clinical manifestations. This study aimed to explore the effect of malaria on biochemical parameters in Sudanese patients with severe falciparum malaria.</p><p><strong>Methods: </strong>A case-control study was conducted in the clinical laboratory of the Kosti Teaching Hospital between August 2022 and January 2023. A total of 400 participants were included in the study and divided into 200 individuals with severe malaria and 200 healthy individuals as the control group. Blood samples were collected from all the participants. Malaria was detected and identified by parasitological techniques. Biochemical parameters were measured and analyzed using an automated biochemical analyzer.</p><p><strong>Results: </strong>Plasma potassium and sodium levels were significantly decreased (p < 0.05), while plasma urea and creatinine levels (p > 0.05) were not affected in infected children compared with controls. Plasma urea levels were significantly higher in the infected adult group than those in the adult controls (p < 0.05) and infected children (p > 0.05). In contrast, the levels of plasma creatinine, potassium, and sodium were not affected in the adult patients (p > 0.05). In addition, there were no significant differences in the levels of plasma urea, creatinine, potassium, or sodium between the sexes of child patients and adult patients (p > 0.05). Additionally, there was a significant positive correlation between urea and sodium (r = 0.322, p < 0.001) and an insignificant correlation between urea, creatinine, and potassium among infected children (r = 0.202, -0.187, p > 0.05) and adults (r = 0.141, 0.096, p > 0.05).</p><p><strong>Conclusions: </strong>Accordingly, we concluded that malaria infection is the risk factor for hyponatremia and hypokalemia among children as well as azotemia among adult patients. Moreover, plasma creatinine, potassium, and sodium levels were not affected by age or gender in child and adult patients. Early detection of electrolyte and renal parameters may help predict and prevent complications of severe malaria.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"44,X,der(21;22)(q10;q10)[43]/45,XX,der(21;22)(q10;q10)[27] a Case Study of Mosaicism with Menstrual Disorders.","authors":"Cong Liu, Yan Lin, Mihua Liu","doi":"10.7754/Clin.Lab.2025.250236","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250236","url":null,"abstract":"<p><strong>Background: </strong>Turner syndrome (TS), also known as congenital ovarian hypoplasia, is one of the most common sex chromosome diseases in women. It is caused by the complete or partial deletion or structural change of one X chromosome in all or part of somatic cells. A rare case of karyotype Turner syndrome is reported.</p><p><strong>Methods: </strong>A 16-year-old female presented with oligomenorrhea and a history of menstrual irregularities. Menarche occurred at age 14, followed by only one menstrual period in the same year. Her second menstruation occurred a year later at age 15, with no menstruation thereafter. Peripheral venous blood was collected after obtaining informed consent. Routine lymphocyte culture and G-banding analysis revealed mosaic karyotypes: 44,X, der(21;22)(q10;q10)[43]/45,XX,der(21;22)(q10;q10)[27].</p><p><strong>Conclusions: </strong>Menstrual disorders are very common nowadays and generally related to personal stress, endocrine system, etc., and may also be related to sex chromosomes. This case is caused by the abnormal structure and number of sex chromosomes.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Value of Combining Padua Score with D-Dimer for Deep Vein Thrombosis in Acute Ischemic Stroke Patients with Alteplase.","authors":"Yanping Lin, Meifang Hua, Chunzhen Zhang, Chunxia Hu, Feng Peng, Xingyu Chen","doi":"10.7754/Clin.Lab.2025.250152","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250152","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the predictive value of combining the Padua score with D-dimer levels for identifying lower extremity deep vein thrombosis (DVT) in acute ischemic stroke (AIS) patients undergoing intravenous thrombolysis with alteplase.</p><p><strong>Methods: </strong>This retrospective study analyzed clinical data from 632 AIS patients who received alteplase treatment at our hospital between September 2019 and October 2023. After applying strict inclusion and exclusion criteria, a total of 172 patients were included in the analysis: 35 patients in the DVT group and 137 patients in the non-DVT group. The area under the receiver operating characteristic curve (AUC) was used to compare the predictive per-formance of the Padua score, D-dimer levels, and their combination.</p><p><strong>Results: </strong>Both the Padua score and D-dimer levels were significantly higher in the DVT group compared to the non-DVT group (p < 0.05). The individual AUC values for the Padua score and D-dimer were 0.805 and 0.691, respectively, with no statistically significant difference between them (p > 0.05). However, when combined, the AUC value increased to 0.828, with a Youden index of 0.591, sensitivity of 0.686, and specificity of 0.905. These metrics were all significantly higher than those obtained using the Padua score or D-dimer alone, demonstrating a statistically significant improvement in predictive accuracy (p < 0.05).</p><p><strong>Conclusions: </strong>The combination of the Padua score and D-dimer levels can serve as an effective early warning tool for DVT in AIS patients treated with intravenous alteplase thrombolysis, thereby enhancing the early detection and management of DVT in this patient population.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Analysis of the Immune-Related lncRNA-miRNA-mRNA Network and Identification of a Novel Potential Biomarker for ISR.","authors":"Xia Zhao, Long Li, Shan Jin, Yufei Feng, Yinfei Lu, Yankai Xu, Shiwei Xie, Lijuan Pang, Kejian Liu","doi":"10.7754/Clin.Lab.2025.241243","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241243","url":null,"abstract":"<p><strong>Background: </strong>The lncRNA-miRNA-mRNA regulatory network is recognized for its significant role in cardiovascular diseases, yet its involvement in in-stent restenosis (ISR) remains unexplored. Our study aimed to investigate how this regulatory network influences ISR occurrence and development by modulating inflammation and immunity.</p><p><strong>Methods: </strong>By utilizing data extracted from the Gene Expression Omnibus (GEO) database, we constructed the lncRNA-miRNA-mRNA regulatory network specific to ISR. We constructed a prediction model using least absolute shrinkage and selection operator (LASSO) analysis and validated it using a nomogram and receiver operating characteristic (ROC) curve analysis. For the top differentially expressed genes (DEGs), we performed functional enrichment analyses, including Gene Ontology (GO) biological processes, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and constructed a protein-protein interaction (PPI) network. Additionally, we employed CIBERSORT to perform immunoinfiltration analysis to elucidate the relationship between DEG and immune cells.</p><p><strong>Results: </strong>CAPN1 was identified as the optimal mRNA, corresponding to the top DEG. We developed a diagnostic prediction model with an area under the receiver operating characteristic curve (AUC) of 0.93333, indicating robust predictive performance of the model. Subsequently, the results of GO and KEGG indicated that CAPN1-related co-expression genes were predominantly enriched in the PI3K-Akt signaling pathway, with GSK3B and BRCA1 identified as key nodes within the PPI network. Additionally, we employed CIBERSORT to perform immunoinfiltration analysis to elucidate the relationship between CAPN1 and immune cells.</p><p><strong>Conclusions: </strong>CAPN1 has the potential to function as a non-invasive biomarker for the diagnosis of ISR, which influences Tfh and naive B cells via the PI3K/AKT pathway, potentially aiding early diagnosis, treatment, and prognosis of ISR.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a Manual DNA Extraction Method Combined with In-House Aspergillus Real-Time PCR.","authors":"In Young Yoo, Joo An Kwon, Raeseok Lee, Sung-Yeon Cho, Won-Bok Kim, Chulmin Park, Hyun-Soo Seok, Dong-Gun Lee, Yeon-Joon Park","doi":"10.7754/Clin.Lab.2025.250209","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250209","url":null,"abstract":"<p><strong>Background: </strong>To improve the molecular diagnostic yield for Aspergillus spp. from respiratory samples, we developed and evaluated a new DNA extraction method directly from respiratory samples combined with in-house Aspergillus real-time PCR.</p><p><strong>Methods: </strong>We developed a method using beads and resin, where a sample is centrifuged to separate the supernatant and pellet. The pellet undergoes bead beating, is mixed with the supernatant, washed with AL buffer and distilled water, followed by the addition of Chelex-100 resin and boiling to extract the DNA template from the supernatant. As a comparator method, the Qiagen kit was used. To evaluate the efficiency of DNA extraction, nucleic acids extracted by the two methods were tested using in-house Aspergillus real-time PCR, and the cycle threshold (Ct) values were compared. The evaluation was conducted using contrived sputum samples with reference strains and 100 clinical respiratory specimens.</p><p><strong>Results: </strong>Using contrived sputum samples at various concentrations, for A. niger and A. flavus, the Ct values were significantly lower when nucleic acids were extracted using the beads plus resin method compared to the Qiagen kit. For clinical samples, the beads plus resin method demonstrated a higher sensitivity of 88% (44/50) for the pan-Aspergillus primer/probe set compared to 76% (38/50) with the Qiagen kit, and significantly lower Ct values were obtained with our method (p < 0.001).</p><p><strong>Conclusions: </strong>We developed a DNA extraction method that outperforms a commercial kit, enabling effective molecular detection of Aspergillus spp. directly from respiratory specimens to aid in diagnosing invasive pulmonary aspergillosis.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on Disease Classification and Small Sample Size in Saudi Anemia-Leukemia Study of Newly-Diagnosed Patients.","authors":"Haiying Hu, Linjun Wang","doi":"10.7754/Clin.Lab.2025.250633","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250633","url":null,"abstract":"","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of Cytomegalovirus Reactivation: Viral Load Distribution Across Several Factors.","authors":"Faiha A Alharbi, Mazen Almehmadi, Mamdouh Allahyani, Ahmad K Shawush, Osama Abdulaziz, Naif Alsiwiehri, Meshari A Alsuwat","doi":"10.7754/Clin.Lab.2025.250219","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250219","url":null,"abstract":"<p><strong>Background: </strong>Cytomegalovirus (CMV) viremia is a critical concern and known by the presence of the virus DNA in the blood, which poses sever risks and develops many complications in immuno-compromised patients. When CMV is untreated, it can cause pneumonitis, colitis, hepatitis, and encephalitis. Current diagnosis relies on molecular methods with qPCR as the preferred method.</p><p><strong>Methods: </strong>The study included 1,050 participants from Taif city, with only detected cases analyzed. Age groups were categorized as 2 - 8, 27 - 49, 50 - 75, and > 75 years. Blood samples (5 mL) were collected from patients at armed forces hospitals from January 2023 to March 2024. Viral DNA detection used COBAS 5800, and statistical analysis applied the chi-squared test.</p><p><strong>Results: </strong>This study analyzed 1,050 samples, 21.7% detected cases equal to 228 patients. Variation and significant results were detected in the adult age group 27 - 49, mainly from the kidney transplant ward. Low viral load was found to be the highest and primarily among kidney transplantation and family medicine wards. Neonatal ICU has several cases of moderate CMV viral load. Males were more affected than females by CMV reactivation.</p><p><strong>Conclusions: </strong>Our results highlight the prevalence and distribution of CMV by age group and hospital ward setting, and they are consistent with the literature. Controlling CMV is critically important. In immunocompromised patients the reactivation can cause morbidity, and effective CMV management is necessary. Neonates are highly susceptible to growth failure, neurodevelopmental impairment, sensorineural hearing loss, and vision deficits if CMV is untreated. In transplant recipients, uncontrolled CMV replication may induce graft rejection, disseminated inflammation, and opportunistic infections. Molecular diagnostics enable early detection and appropriate antiviral treatment.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mantle Cell Lymphoma with Severe Hyperleukocytosis and Bone Marrow Infiltration.","authors":"Shanmei Lv, Hongkun Xv, Zhe Chen, Yi Feng","doi":"10.7754/Clin.Lab.2024.250251","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.250251","url":null,"abstract":"<p><strong>Background: </strong>Mantle cell lymphoma (MCL) is a subtype of non-Hodgkin lymphoma characterized by the proliferation of B-cells. Severe hyperleukocytosis is an uncommon presentation that can complicate the diagnosis and management of MCL.</p><p><strong>Methods: </strong>We present the case of an 80-year-old male patient suffering from MCL, who exhibited symptoms including severe hyperleukocytosis, lymphadenopathy, and significant bone marrow infiltration. Diagnostic procedures included complete blood count, bone marrow biopsy, lymph node needle biopsy, and flow cytometry to confirm the diagnosis.</p><p><strong>Results: </strong>The patient exhibited a leukocyte count exceeding 491.28 x 109/L, 65.0% abnormal cell in peripheral blood, with bone marrow analysis revealing over 90% infiltration by mature B-cells. Immunohistochemical results of lymph node biopsy were considered for CyclinD1 (+) mantle cell lymphoma.</p><p><strong>Conclusions: </strong>This case highlights the need for careful differential diagnosis between MCL and other lymphoblastic leukemias in patients presenting with severe hyperleukocytosis. Accurate identification is essential for appropriate management and treatment.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of Pulmonary Actinomycosis Caused by Actinomyces graevenitzii: a Case Series of Three Patients.","authors":"Xiaojiao Zhang, Qiuli He, Guofeng Mao, Meichun Liang","doi":"10.7754/Clin.Lab.2025.250254","DOIUrl":"10.7754/Clin.Lab.2025.250254","url":null,"abstract":"<p><strong>Background: </strong>Actinomyces graevenitzii is a relatively uncommon Actinomyces species, which is an oral species and predominantly recovered from respiratory locations [1,2]. It is a gram-positive anaerobic bacteria or microaerobic filamentation bacteria, which can induce pyogenic and granulomatous inflammation characterized by swelling and concomitant pus, sinus formation, and the formation of yellow sulfur granules. All tissues and organs can be infected; the most common type involves the neck and face (55%), followed by the abdominal and pelvic cavities (20%). Chest involvement is the third most common type (15%), affecting the lung parenchyma, central airway, pleura, mediastinum, and chest wall [3-5]. Diagnosis of actinomycosis mainly relies on sputum, pus or biopsy specimens to find actinomycetes. Metagenomics next-generation sequencing (mNGS) in recent years has been increasingly valued and recognized for its application in infectious diseases. It provides a more efficient and accurate means for the pathological diagnosis of respiratory infections, updating the diagnostic strategy for lower respiratory tract infections [6].</p><p><strong>Methods: </strong>This study systematically summarized the clinical characteristics of Actinomyces graevenitzii infection by analyzing three cases of this pathogen. Bronchoalveolar lavage fluid (BAL) samples were collected for bacterial culture and mNGS. The isolated strains were routinely identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).</p><p><strong>Results: </strong>Microbiological analysis demonstrated Actinomyces graevenitzii growth in all three bronchoalveolar la-vage (BAL) cultures. Metagenomic next-generation sequencing (mNGS) results showed concordant detection of A. graevenitzii in two cases, while unexpectedly identifying Tropheryma whipplei as the predominant pathogen in the remaining case.</p><p><strong>Conclusions: </strong>Actinomycosis infections often present with subtle and nonspecific clinical manifestations, making them difficult to distinguish from pulmonary tuberculosis, fungal infections, and lung malignancies through imaging studies alone, frequently resulting in misdiagnosis or delayed diagnosis. Early and accurate diagnosis can be achieved through timely analysis of BAL fluid using advanced diagnostic techniques, including MALDI-TOF/MS for bacterial identification and mNGS, facilitating prompt and appropriate treatment.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Blast Detection in Leukopenic Patients by Identifying Apoptotic Regions in the Sysmex XN-Series WPC Scattergram.","authors":"Hyun Lee, Jae Kyung Kim, Yoon Hwan Chang","doi":"10.7754/Clin.Lab.2025.250203","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250203","url":null,"abstract":"<p><strong>Background: </strong>The white cell precursor (WPC) channel of the Sysmex XN-series hematology analyzer, which is designed for blast detection, showed reduced sensitivity for blast detection in leukopenic patients undergoing chemotherapy. This study aimed to evaluate the gating region for apoptotic blasts in the WPC scattergram to enhance detection sensitivity.</p><p><strong>Methods: </strong>NOMO-1 cells, a human acute monoblastic leukemia cell line, were treated with varying concentrations of cytarabine (0, 100, 500, and 1,000 nM) for three days to induce apoptosis. Apoptotic cells were identified using annexin V and propidium iodide staining and were analyzed by flow cytometry to distinguish early and late apoptosis. Treated cells were mixed with normal blood and analyzed using the WPC scattergram to compare their gating regions with the reference region.</p><p><strong>Results: </strong>Treatment with 1,000 nM cytarabine induced significant apoptosis in NOMO-1 cells, with 30.79% under-going early apoptosis and 38.44% late apoptosis, compared to untreated cells (4.52% early, 5.12% late). Apoptotic NOMO-1 cells were consistently localized in the lower-middle region of the WPC scattergram, distinct from the predefined reference gating region. This pattern persisted in mixed blood samples, confirming their consistent po-sitioning outside the reference gating region.</p><p><strong>Conclusions: </strong>Apoptotic NOMO-1 cells exhibited reduced forward scatter light, side fluorescence light, and side scatter light signals in the WPC scattergram. Incorporating this region into gating strategies may enhance the sensitivity for detecting atypical blasts following chemotherapy, particularly when Wright-Giemsa stains reveal blasts without a corresponding 'Blast?' flag.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 9","pages":""},"PeriodicalIF":0.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}