Clinical laboratory最新文献

{"title":"The Prevalence of Transfusion-Transmitted Infections and Nucleic Acid Testing Among Blood Donors in Makkah, Saudi Arabia.","authors":"Mohammad H Albshri, Ahmad A Ghoth, Abdulqader A Mutwalli, Naif S Alzahrani, Samer S Bahattab, Bassam A Hanbali, Mohammed H Althaqafi, Abdulaziz M Alqarni, Mansor M Alsulimani, Naif K Alzahrani, Fahad S Alharbi, Wafaa H Alansari, Abdulelah A Ekram, Ali A Alghamdi, Mansour A Alzahrani","doi":"10.7754/Clin.Lab.2024.240915","DOIUrl":"10.7754/Clin.Lab.2024.240915","url":null,"abstract":"<p><strong>Background: </strong>Despite saving a vast number of lives through blood transfusions, transfusion-transmitted infections (TTIs) still threaten the lives of people needing blood transfusion. Hence, screening blood donors and reviewing the prevalence of TTIs amongst blood donors might show the impact of these infections among our people. The aim of this study was to evaluate the prevalence rates of transfusion-transmitted infections among blood donors in Makkah as foundation for providing harmless blood transfusion in Makkah, Saudi Arabia.</p><p><strong>Methods: </strong>A retrospective study was carried out at the Central Blood Bank in Makkah city, Saudi Arabia, from January 1, 2023, to December 31, 2023. A total of 13,706 samples were collected and screened for hepatitis B surface antigen (HBsAg), hepatitis B surface antibodies (HBsAbs), hepatitis B core antibodies (HBcAbs), antibodies to hepatitis C virus (anti-HCV), antibodies to HIV 1 and 2 (HIV I/II Ab), antibodies to HTLV 1 and 2 (HTLV I/II Ab), Malarial antibodies, and antibodies to VDRL.</p><p><strong>Results: </strong>A total of 13,706 blood units were received and tested. Out of the total, 52% were non-Saudi donors and 47.9% were Saudi donors; 28.4% were aged 18 - 28 years, 42.8% were aged 29 - 39 years, 24.4% were aged 40 - 50 years, and 4.4% were aged 51 - 60 years. The majority was O+ blood group (42.7%), followed by A+ (26.2%), B+ (18.7%), AB+ (4.6%), O- (3.8%), A- (2.1%), B- (1.5%), and AB- (0.4%). While 42.6% of the blood units donated were from voluntary donors, 57.4% were donated by replacement donors. Sixty-one samples (0.4%) tested positive for HBsAg, 824 samples (6%) for HBcAb, 43 samples (0.3%) for HCV antibodies, 754 samples (5.5%) for HBsAb, and 44 samples (0.3%) for HIV I/II Ag/Ab combinations. Further, 44 samples (0.3%) were positive for HTLV I/II antibodies, 83 samples (0.6%) for VDRL antibodies, and only 3 samples (nearly 0%) for malaria antibodies. Forty-three samples (0.3%) were positive for NAT-HBV, 7 samples (0.1%) were positive for NAT-HCV, and 6 samples (0.1%) were positive for NAT-HIV. The analysis revealed a statistically significant and strong correlation between HBsAgs and NAT-HBV (r = 0.819, p < 0.0001). In contrast, while there was a statistically significant association between HBsAgs and HBcAbs, the correlation was weak (r = 0.191, p < 0.0001). Additionally, there was an association between HBsAbs and HBsAgs, but the Spearman correlation indicated a very weak relationship (r = 0.042, p < 0.0001).</p><p><strong>Conclusions: </strong>Prevalence rates of transfusion-transmitted infections showed a steady decline in 2023, and these rates were much lower in Makkah than in other parts of the country or in neighboring countries. The importance of using NAT in the screening of blood donors was indicated in this study. These findings could contribute to improving the understanding of TTIs epidemiology and supporting health authorities in controlling blo","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causes and Management Strategies for Unusual Hypoglycemia: a Case Series.","authors":"Guoxiang Bao, Jialu Li, Yunan Mei, Cuiying Yang","doi":"10.7754/Clin.Lab.2024.240533","DOIUrl":"10.7754/Clin.Lab.2024.240533","url":null,"abstract":"<p><strong>Background: </strong>Glucose levels are vital for indicating the body's sugar content, with imbalances leading to diseases like diabetes or hypoglycemia-related symptoms such as palpitations and fatigue.</p><p><strong>Methods: </strong>This case series describes three cases of hypoglycemia identified in recent years, utilizing multiple glucose measurement methods and exploring strategies to eliminate interferences.</p><p><strong>Results: </strong>Two cases of pseudo-hypoglycemia induced by PEGylated recombinant human granulocyte colony-stimulating factor (PEG-rhG-CSF) and Evolocumab injections, and one case of true reactive hypoglycemia following a glucose tolerance test in a patient post-gastric bypass surgery.</p><p><strong>Conclusions: </strong>For unusual hypoglycemia, collaboration with clinicians and multiple methods is crucial for accurate analysis to differentiate true from pseudo-hypoglycemia, ensuring precise diagnosis and optimal clinical service.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Diagnostic Accuracy in Hepatitis B: Human vs. Sheep-Derived Antibodies in HBsAg Confirmatory Testing.","authors":"Seunghwan Kim, Min-Kyung So, Tae-Dong Jeong, Soo-Kyung Kim","doi":"10.7754/Clin.Lab.2024.240527","DOIUrl":"10.7754/Clin.Lab.2024.240527","url":null,"abstract":"<p><strong>Background: </strong>HBsAg confirmatory testing is crucial for accurately diagnosing HBV infection and avoiding false positives. We compared the performance of human-derived and sheep-derived anti-HBs reagents in the Elecsys HBsAg confirmatory test (Roche Diagnostics, Germany).</p><p><strong>Methods: </strong>Samples with a HBsAg COI of 0.9 or more underwent confirmatory testing with both reagents. Results showed a high overall agreement rate of 98% between human and sheep reagents.</p><p><strong>Results: </strong>The average confirmation percentage with sheep anti-HBs was significantly lower than with human anti-HBs (6.9% vs. 20.0%, p = 0.002). Additionally, some strongly positive HBsAg samples could not be neutralized.</p><p><strong>Conclusions: </strong>This suggests that the polyclonal antibodies in these reagents have different specificities and affinities for HBsAg epitopes. The findings emphasize the importance of reagent specificity and affinity to ensure accurate HBV diagnosis. Additional HBV markers and clinical context must be considered to confirm the diagnosis.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic Analysis of the Structural Features of the H106P/N137A Mutant as a Potential Vaccine Candidate Against Clostridium perfringens.","authors":"Elmira Nakhaipour, Mojtaba Alimolaei, Ashraf Kariminik, Mehrdad S Bafti","doi":"10.7754/Clin.Lab.2024.240220","DOIUrl":"10.7754/Clin.Lab.2024.240220","url":null,"abstract":"<p><strong>Background: </strong>The complex and expensive processes involved in the production of current clostridial toxoid vaccines require the design of new methods. The aim of this study was to introduce single and combined nontoxic mutants of Clostridium perfringens epsilon toxin (ETX) into the vaccine production.</p><p><strong>Methods: </strong>Antigenic properties, amino acid sequence, physicochemical characteristics, stability, and protein structure of individual and combined ETX mutants were investigated with bioinformatics software. Spatial structure prediction was done by using a homologous modeling method based on the structure of wild type ETX (1UYJ1A.pdb) and the validity of the drawn spatial model was evaluated by estimating the quality and accuracy of the spatial chemistry of the modeled mutant proteins by Ramachandran plot.</p><p><strong>Results: </strong>The highest instability index was observed in H106P/N137A mutant. The results of the homology modeling did not show a clear structural change in any of the mutants compared to the wild ETX. The percentages of amino acids in the favored regions, allowed regions, and outlier regions were 92.81%, 5.03%, and 2.16%, respectively, which indicate the desirability of the proposed model for the three-dimensional structure of the H106P/N137A hybrid mutant.</p><p><strong>Conclusions: </strong>Recombinant H106P/N137A mutant of ETX can be considered a suitable candidate for the production of epsilon genetic toxoid, and this requires extensive laboratory evaluations.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case of Abnormal Elevation of Serum CA 72-4 Caused by Colchicine.","authors":"Gangfeng Li, Tao Lu","doi":"10.7754/Clin.Lab.2024.240601","DOIUrl":"10.7754/Clin.Lab.2024.240601","url":null,"abstract":"<p><strong>Background: </strong>CA 72-4 is a tumor associated antigen which shows significantly increased serum levels in gastric cancer. It is the most commonly used serum tumor marker for diagnosing symptomatic gastric cancer and monitoring gastric cancer treatment.</p><p><strong>Methods: </strong>We report the case of a gout patient with abnormal elevation of serum CA 72-4 caused by taking colchicine.</p><p><strong>Results: </strong>The patient's painless gastroscopy and other examinations showed no obvious abnormalities, and no detection interference was detected. After discontinuing treatment with colchicine, CA 72-4 level of the patient returned to normal. Therefore, the abnormal increase of CA 72-4 is believed to be caused by colchicine.</p><p><strong>Conclusions: </strong>When gout patients receive colchicine treatment, it may lead to an abnormal increase in CA 72-4 results. Laboratory staff should consider the existence of such interference and promptly remind clinical doctors to avoid unnecessary examination and treatment of patients.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Immunochromatographic Test for Rapid Detection of Norovirus, Rotavirus, and Adenovirus in Stool Samples.","authors":"Nutthawadee Jampanil, Thitapa Longum, Kattareeya Kumthip, Arpaporn Yodmeeklin, Nuthapong Ukarapol, Akiko Nomura, Yuko Nomura, Shoko Okitsu, Niwat Maneekarn, Hiroshi Ushijima, Pattara Khamrin","doi":"10.7754/Clin.Lab.2024.240534","DOIUrl":"10.7754/Clin.Lab.2024.240534","url":null,"abstract":"<p><strong>Background: </strong>Viral enteric infections are illnesses caused by several types of viruses that primarily affect the gastrointestinal system. Globally, three major viruses associated with gastroenteritis, including norovirus (NoV), rotavirus A (RVA), and human adenovirus (HAdV), have been widely recognized. Accordingly, a rapid and sensitive diagnostic tool, such as immunochromatographic (IC) test, for diarrheal virus detection is required and helpful for rapid diagnosis.</p><p><strong>Methods: </strong>The IP-Triple I IC test kit for simultaneous triple virus detections of NoV, RVA, and HAdV was evaluated for its efficacy by using stool specimens that were known positive for these viruses by real-time RT-PCR or PCR, which was used as the gold standard method.</p><p><strong>Results: </strong>The results revealed that the IP-Triple I IC test kit exhibited a very high level of specificity (100%) for NoV, RVA, and HAdV detections, while the sensitivity was slightly different for these three viruses. The sensitivity of detection for RVA was 86.7%, whereas those for NoV and HAdV were 70.6% and 76.2%, respectively. This IP-Triple I IC kit could detect common antigens of a wide range of NoV, RVA, and HAdV genotypes (NoV GII.2, GII.4, GII.6, GII.7, GII.10, GII.17, RVA G1P[8], G2P[4], G3P[8], G8P[8], G9P[8], HAdV-C1, -C2, -C5, -A12, -F40, and -F41).</p><p><strong>Conclusions: </strong>Our results revealed that the IP-Triple I IC test kit is highly effective for simultaneous, direct detection of common antigens of several genotypes of NoV, RVA, and HAdV in stool specimens and useful for screening and rapid diagnosis of diarrheal viruses during the seasonal outbreak.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexmedetomidine Alleviates Ferroptosis Induced by Sepsis-Induced Renal Injury by Activating Keap1-Nrf2 Signaling Pathway.","authors":"Yan Yan, Zhigao Zhu, Haofeng Ding, Xingchun Zhu, Jiahao Zhang, Chengwen Fu, Dandan Li, Jiaxin Chu, Li Ren, Congli Zhang","doi":"10.7754/Clin.Lab.2024.240539","DOIUrl":"10.7754/Clin.Lab.2024.240539","url":null,"abstract":"<p><strong>Background: </strong>The purpose of this study was to investigate the protective effect of dexmedetomidine (DEX) on sep¬sis-induced acute kidney injury (AKI) and its possible mechanisms.</p><p><strong>Methods: </strong>A total of 40 mice were randomly divided into the control group (C group), lipopolysaccharide treatment group (LPS group), LPS+DEX group, and ferrostatin-1 group (LPS+Fer-1 group). Mice in the LPS group were intraperitoneally injected with LPS (10 mg/kg), while mice in the LPS+DEX and LPS+Fer-1 groups were intraperitoneally injected with Dex (30 μg/kg) and Fer-1 (10 mg/kg), 1 hour before LPS injection, respectively. Mice in the control group were infused with the same volume of saline. Serum creatinine (SCr), blood urea nitrogen (BUN) and the levels of superoxide dismutase (SOD), malondialdehyde (MDA), and total antioxidant capacity (T-AOC) in renal tissue were measured. HE staining was used to evaluate the degree of kidney tissue injury. Immunohistochemistry and western blot were used to detect the protein expressions of FTH, TFR, Keap1, and Nrf2 in kidney tissue.</p><p><strong>Results: </strong>Compared with the control group, the serum levels of SCr and BUN were significantly increased, the levels of SOD and T-AOC in the kidney were decreased, the MDA level and renal injury score were increased, the expression of FTH and Nrf2 protein was reduced, and the expression of TFR and Keap1 protein was increased in the LPS group (p < 0.05). Compared with the LPS group, the serum levels of SCr and BUN were significantly de-creased, the levels of SOD and T-AOC in the kidney were increased, the MDA level and renal injury score were decreased, the expression of FTH and Nrf2 protein was increased, and the expression of TFR and Keap1 protein was decreased in the LPS+DEX group (p < 0.05).</p><p><strong>Conclusions: </strong>Dex can alleviate sepsis-associated acute kidney injury by activating the Keap1/Nrf2 pathway.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics and Prognostic Factors for Adult HIV-Negative Burkitt Lymphoma: a Single-Center Retrospective Study.","authors":"Guanzhen Lai, Rongshan Zhang","doi":"10.7754/Clin.Lab.2024.240347","DOIUrl":"10.7754/Clin.Lab.2024.240347","url":null,"abstract":"<p><strong>Background: </strong>We performed a retrospective cohort study to examine the clinical characteristics, prognosis factors, and survival outcomes of HIV-negative adult Burkitt's lymphoma.</p><p><strong>Methods: </strong>The retrospective study was conducted on adult patients, who were diagnosed with HIV-negative Burkitt lymphoma at our center between 2014 and 2022. Univariate and multivariate Cox regression analyses were conducted to identify potential risk factors for mortality. Survival rates were estimated, using the Kaplan-Meier curve and the log-rank test.</p><p><strong>Results: </strong>A total of 23 patients were identified, with male patients making up the majority (69.6%). Over half (56.5%) of the patients had bone marrow involvement, while a third (30.4%) had central nervous system involvement. More than half of the patients (13/23) were given R-hyper CVAD as their first-line therapy. The median PFS and OS were 11 and 12 months, respectively. In multivariate analysis, central CNS involvement was found to be an independent predictor of worse OS among patients with BL, with a hazard ratio of 15.53 (95% CI: 1.09 - 22.5). We also found that patients with CNS involvement were more likely to have higher LDH (p = 0.045) and ECOG scores (p = 0.002).</p><p><strong>Conclusions: </strong>Our research revealed that CNS is an important predictor of OS and DFS. LDH > 3ULN levels were associated with worse PSF. This study is valuable, because it shows the characteristics of a population that is currently poorly studied and has a lower incidence in China.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Distinct Genotype, D/Ep6, Detected in Korean Female Patients: Chlamydia trachomatis Characterization from 2017 - 2018.","authors":"Yeon-Joo Choi, Taeuk Kang, Kyoung Ho Roh, Won-Jong Jang","doi":"10.7754/Clin.Lab.2024.240609","DOIUrl":"10.7754/Clin.Lab.2024.240609","url":null,"abstract":"<p><strong>Background: </strong>The objective of this study lies in identifying the dominant genotype of C. trachomatis isolated in Republic of Korea (ROK) between 2017 and 2018.</p><p><strong>Methods: </strong>A total of 504 clinical cervicovaginal swabs from patients were collected and inoculated on McCoy cell monolayers to isolate Chlamydial agents. C. trachomatis isolates were analyzed by sequencing of its ompA gene.</p><p><strong>Results: </strong>A total of 54 C. trachomatis isolates were obtained. The constructed phylogenetic tree revealed the genotypes of isolates are D/Ep6 (48, 88.89%), D/Ep6-like (5, 9.26%), and Ja (1, 1.85%). The C. trachomatis D/Ep6-like have one single nucleotide polymorphism (SNP) compared to C. trachomatis D/Ep6, leading to E870K amino acid change.</p><p><strong>Conclusions: </strong>Phylogenetic analysis demonstrated globally rare C. trachomatis D/Ep6 was dominant in the ROK from 2017 to 2018. Findings in this study act as a keystone, bridging past and present molecular epidemiology of C. trachomatis in context of ROK.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of External and Internal Site Genital Sampling to Detect High-Risk HPV DNA in Women.","authors":"Esin Kasap, Can Turkler, Nisel Yilmaz, Goksen Gorgulu, Alper Togay, A Hamdi Inan, Mehmet Sarier","doi":"10.7754/Clin.Lab.2024.240724","DOIUrl":"10.7754/Clin.Lab.2024.240724","url":null,"abstract":"<p><strong>Background: </strong>Human papillomavirus (HPV), the most common pathogen causing sexually transmitted diseases worldwide, is also an oncogenic virus. Due to the inadequacy of serologic tests in the diagnosis of HPV, NAATs (nucleic acid amplification tests), such as PCR (polymerase chain reaction), represent the gold standard today. Endocervical brush sampling in women has been successfully used for HPV genotyping for many years. The aim of this study was to determine the diagnostic efficacy for PCR HPV genotyping of multisite samples taken simultaneously with a swab from the external genitalia in addition to endocervical brush sampling in women applying for Hr-HPV screening.</p><p><strong>Methods: </strong>This study included 105 asymptomatic patients who came to the Gynecology Polyclinic of the University of Health Sciences Tepecik Training and Research Hospital between February 2023 and June 2023 for control purposes. Both the samples taken with a brush from the cervical area and the samples taken with a swab from the external genital area were sent to a screening laboratory for testing for Hr-HPV DNA. The samples were analyzed by real time PCR.</p><p><strong>Results: </strong>The success rate of positive detection of swab samples of HPV type 16 was significantly higher, with a difference of 17.6% (p < 0.0001). For HPV type 18, the swab sample had a significantly higher positive detection rate, with a difference of 60.0% (p = 0.002).</p><p><strong>Conclusions: </strong>The results of this study show that HPV genotyping from the external genital area can be performed as an alternative to cervical sampling in sexually active women to increase the reach of a screening program.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}