Clinical laboratory最新文献

{"title":"An ITP Patient Taking Eltrombopag Presented with Extreme Thrombocytosis after Orthopedic Surgery.","authors":"Xiuping Xu, Guoxing Ge","doi":"10.7754/Clin.Lab.2024.241218","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241218","url":null,"abstract":"<p><strong>Background: </strong>Primary immune thrombocytopenia (ITP) is an acquired autoimmune disease characterized by varying degrees of platelet count reduction, with or without skin and mucosal bleeding. Eltrombopag is a thrombopoietin receptor agonist (TPO-RA) that promotes platelet production by specifically binding to the transmembrane domain of thrombopoietin receptor (TPO-R).</p><p><strong>Methods: </strong>Blood routine examination was performed to monitor the changes of platelets in this ITP patient during the treatment of eltrombopag.</p><p><strong>Results: </strong>The ITP patient had an extreme thrombocytosis after open reduction and internal fixation of radial fracture while taking eltrombopag.</p><p><strong>Conclusions: </strong>We believe that this rare case was caused by the co-stimulation of eltrombopag, surgery, inflammation, and artificial bone implant. Therefore, ITP patients taking eltrombopag should monitor platelet changes, especially during infection and surgery, so as to adjust the dose of eltrombopag in time.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Gleason Patterns with α-Methylacyl-Coa Racemase (AMACR) Immunohistochemistry Reaction.","authors":"Mihriban Gürbüzel, Berna Eriten, Meryem Yüvrük, Gülce S Yildiz, Dilek Yavuzer","doi":"10.7754/Clin.Lab.2024.241202","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241202","url":null,"abstract":"<p><strong>Background: </strong>Prostate cancer, with a reported prevalence of 35 per hundred thousand in Türkiye, ranks third among the most frequently encountered cancers worldwide. In incisional needle biopsies, it is a challenge to identify malignant glands that are very few or sometimes mixed within benign changes such as adenosis and atrophy. AMACR is a marker that is seen to have a higher expression in the malignant lesions of the prostate compared to a normal prostate. In this study, we aimed to retrospectively report the cases where we used this marker, for which we believe that it strongly supports findings of malignancy in our daily routine, and present our findings along with other findings in the literature.</p><p><strong>Methods: </strong>We searched prostate needle biopsy cases in which AMACR was tested as an immunohistochemistry method between July 2022 and June 2023 on the digital system of the hospital. The study included 104 cases consisting of the diagnoses of prostate adenocarcinoma and ASAP (atypical small acinar proliferation), which included 8 HGPIN cases. Patient ages were also recorded. The cases were reevaluated based on their staining grades, rated as none: 0, weak: 1, moderate: 2, and strong: 3, in addition to the areas of staining in percentages. The collected data were analyzed using the SPSS (Statistical Package for the Social Sciences) for Windows 22.0 program.</p><p><strong>Results: </strong>All cases were positive for AMACR, and the prevalence was 100%. The AMACR staining grades were moderate by 44.9% in Gleason pattern 3 (P3) cases and 49.1% in Gleason pattern 4 (P4) cases, whereas most Gleason pattern 5 (P5) cases had strong staining at a rate of 63.2%. Weak staining was more frequently encountered in the eight ASAP and eight HGPIN cases, whereas no strong staining was observed in any ASAP case.</p><p><strong>Conclusions: </strong>In the comparisons of patient ages and Gleason patterns, no significant difference was found in terms of staining grades (p > 0.05). There was a 53.6% positive correlation between P3 and P4 staining grades (p < 0.05). There was also an 86.2% positive correlation between P5 and P4 staining grades (p < 0.05).</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of Red Cell Distribution Width, Plasma Albumin, and Serum Amylase as Marker of Severity of Infection in Emergency Patients with Acute Pancreatitis.","authors":"Feng Zhang","doi":"10.7754/Clin.Lab.2024.241031","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241031","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to investigate the predictive values of red cell distribution width (RDW), plasma albumin (ALB), and serum amylase (AMY) for the occurrence of infection in emergency patients with acute pancreatitis (AP) as well as their correlations with the severity.</p><p><strong>Methods: </strong>A total of 121 AP patients with infection and 137 without infection treated between January 2022 and January 2024 were enrolled as infection group and non-infection group, respectively. The infection group was further assigned into a good prognosis group (n = 65) and a poor prognosis group (n = 56). Venous blood (4 mL) was collected in the morning after at least 8 hours of fasting. RDW, plasma ALB level, and serum AMY level were measured through blood cell analysis, enzyme-linked immunosorbent assay, and rate method, respectively.</p><p><strong>Results: </strong>RDW and serum AMY level were positively correlated with computed tomography severity index (CTSI) score, whereas plasma ALB level was negatively correlated with CTSI score (p < 0.05). RDW and serum AMY were independent risk factors for infection in emergency AP patients, and plasma ALB was an independent pro¬tective factor (p < 0.05). The areas under the receiver operating characteristic curves (AUCs) of RDW, plasma ALB, and serum AMY levels for predicting infection in AP patients reached 0.794, 0.770, and 0.812, respectively, while the AUC, sensitivity, and specificity of their combination were 0.907, 86.86%, and 80.99%, respectively.</p><p><strong>Conclusions: </strong>RDW, plasma ALB, and serum AMY were abnormally expressed in AP patients with infection, and they all had significant correlations with the severity. The combined detection of the three indicators had the highest predictive value.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increase of CD4+CD25+FoxP3+Regulatory T Cells in Myeloma.","authors":"Hee Sue Park, Suel Gi Oh, Bo Ra Son, Yong-Pyo Lee, Jihyun Kwon","doi":"10.7754/Clin.Lab.2024.241208","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241208","url":null,"abstract":"<p><strong>Background: </strong>In the tumor microenvironment, regulatory T cells (Treg cells), a small subset of CD4+ T cells, can interrupt antitumor immune reactions by interacting with various other immune cells. The present study aimed to determine the expression level of Treg cells in myeloma compared with that in normal control.</p><p><strong>Methods: </strong>Peripheral blood samples from 90 healthy adults and marrow aspiration samples from 13 patients newly diagnosed with myeloma were collected. Eight-color flow cytometry was performed using a Duraclone IM Treg tube kit. A gating strategy was established based on immunophenotypic characteristics of Treg cells identified as CD3+CD4+CD25+FoxP3+CD39+Helios+ cells.</p><p><strong>Results: </strong>A 2.5-fold increase in Treg cells, based on the CD4+CD25+FoxP3+ combination, was confirmed in the ini-tial myeloma diagnosis compared with the normal control. Additionally, increased Treg cells had a weak positive correlation with urine M-protein levels (r = 0.619, p < 0.05).</p><p><strong>Conclusions: </strong>Thus, an increase in Treg cells appears to potentially predict a worse complication of myeloma based on microenvironmental-related biomarkers.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-Hospital BSL-2 Clinical Laboratory Biosafety Risk Identification and Risk Control.","authors":"Jia Zhao, Weixin Chen, Yanqiu Zhao, Hanwei Cui, Shuo Song","doi":"10.7754/Clin.Lab.2024.241131","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241131","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to explore the management of risk assessment and risk control in the in-hospital biosafety management of BSL-2 clinical pathogenic microorganism laboratory.</p><p><strong>Methods: </strong>Risk analysis was carried out for complex nosocomial pathogen infection risk, poor scientific research skills of medical staff, weak risk awareness, and unreasonable layout of laboratory division.</p><p><strong>Results: </strong>Through effective risk control means, the infection risk of pathogenic microorganism laboratory was reduced, and the safe operation of the laboratory was guaranteed.</p><p><strong>Conclusions: </strong>BSL-2 clinical laboratory should adopt effective risk assessment and risk control, predict the danger source in advance, eliminate or reduce the hidden danger, and ensure the safety to the greatest extent.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Molecular Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Broiler Chickens, Morocco.","authors":"Mohamed Amzil, Kaotar Nayme, Abdelwahid Assaidi, Mohammed Timinouni, Asmaa Dihmane, Abouddihaj Barguigua, Aboubakr Khazaz, Omar Charafeddine, Ahmed A Bousfiha, Assiya El Kettani","doi":"10.7754/Clin.Lab.2024.240804","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.240804","url":null,"abstract":"<p><strong>Background: </strong>The prevalence, the characterization of extended-spectrum β-lactamase (ESBL)-producing Esche-richia coli (E. coli) strains isolated from broiler chickens in the Casablanca-Settat region of Morocco, and their susceptibility to antibiotics were investigated.</p><p><strong>Methods: </strong>A total of 216 intestinal samples were collected (100 samples in 2017 and 116 samples in 2019). E. coli isolates were screened for reduced susceptibility to Ceftazidime (CAZ) and subjected to antibiotic susceptibility testing. ESBL production was confirmed phenotypically and genotypically using PCR to detect blaTEM, blaCTX-M1, and blaSHV genes.</p><p><strong>Results: </strong>Results revealed a high prevalence (83.5%) of E. coli, with a significant decrease in prevalence observed in 2019 compared to 2017 (69%). Reduced susceptibility to Ceftazidime assay showed that 37% (n = 66) of the 178 E. coli isolates were resistant to Ceftazidime (CAZ-R), which indicates the potential likelihood of having ESBL. ESBL phenotypic testing showed a prevalence of 10.32%, with a slight increase in 2019 (11.5%), compared to 2017 (9%, p > 0.05). Antibiotic susceptibility testing demonstrated high resistance levels, notably in 2019, observed for Amoxicillin (94%), Ceftazidime (81%), and Aztreonam (69%). Multidrug resistance (MDR) significantly increased from 2017 to 2019, with 93% of isolates tested in 2019 resistant to at least three antibiotic families. Genotypic analysis revealed the presence of the blaCTX-M15 (n = 1) and blaTEM1 (n = 2) genes in 2017, with an increase of blaTEM1 in 2019 (n = 9).</p><p><strong>Conclusions: </strong>These findings highlight the emergence and the increases of ESBL-producing E. coli strains in Moroccan broiler chickens, signaling a growing concern for antimicrobial resistance in poultry farming. Enhanced surveillance and control measures are imperative to mitigate the spread of resistant pathogens in both animal and human populations.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case Report and Literature Review of Hemophagocytic Syndrome Secondary to Garcin-Guillain Syndrome.","authors":"Zhong-Yu Li, Yuan-Yuan Tan, Meng-Meng Zhang, Hui Li, Liang Yin, Jia-Jia Li","doi":"10.7754/Clin.Lab.2024.241221","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241221","url":null,"abstract":"<p><strong>Background: </strong>This article reports on a case of hemophagocytic syndrome (HPS) secondary to multiple myeloma (MM) with extramedullary lesions presenting as the initial symptom. MM with extramedullary lesions, also referred to as Garcin-Guillain syndrome, is extremely rare, occurring in only 4.6% of MM patients. Secondary HPS is uncommon in malignant tumors and is particularly rare in MM.</p><p><strong>Methods: </strong>The diagnosis was based on the patient's clinical presentation and diagnostic findings, including blood tests, ferritin levels, head MRI, pathological analysis, and bone marrow biopsy. The patient was diagnosed with MM complicated by Garcin-Guillain syndrome, with HPS secondary to recurrent MM.</p><p><strong>Results: </strong>The patient was hospitalized in September 2020 due to swallowing difficulty, hoarseness, choking while eating for over 10 days, and rightward deviation of the mouth for five days. These symptoms were attributed to the direct spread of MM lesions to the lingual pharyngeal and vagus nerves. PAD chemotherapy (bortezomib, doxorubicin, and dexamethasone) was initiated. After one cycle, the symptoms of Garcin-Guillain syndrome resolved, and partial remission (PR) was achieved after five cycles. In October 2021, the patient was readmitted with recurrent high fever. Clinical findings and examinations met the diagnostic criteria of HLH-2004, confirming secondary HPS due to MM relapse. Treatment with bortezomib, dexamethasone, etoposide, and lenalidomide showed limited efficacy, and the patient subsequently died.</p><p><strong>Conclusions: </strong>HPS secondary to MM recurrence is extremely rare, characterized by severe disease, rapid progression, poor treatment response, and poor prognosis with a short survival time. Despite advances in drug therapies, there is no established treatment protocol for such cases. Further research is needed to improve the outcomes of these patients.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of a Training Model for Laboratory Personnel Based on ISO 15189.","authors":"Yuan Xu, Hong Yu, Juan Chen, Yaoyao Tong, Yali Wang, Ying Liang, Shimin Wu","doi":"10.7754/Clin.Lab.2024.241134","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241134","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to explore the implementation of an effective training and competency assessment model for laboratory personnel in compliance with ISO 15189 standards, thereby enhancing laboratory testing capabilities and providing a reference for personnel training in other hospital laboratories.</p><p><strong>Methods: </strong>A comprehensive training program was developed based on ISO 15189 requirements and the practical experience of our department. The program included determining training schedules and frequencies, defining training content, conducting training sessions, evaluating training outcomes, and performing assessments and competency evaluations.</p><p><strong>Results: </strong>Through the implementation of the proposed training program, we successfully met ISO 15189 requirements for personnel training and competency assessment. The execution of the training plan significantly improved staff professional skills and professional ethics, further strengthening the laboratory's testing capabilities.</p><p><strong>Conclusions: </strong>Implementing personnel training and competency assessment in laboratory departments in accordance with ISO 15189, combined with a scientifically designed training program, effectively enhances staff expertise and laboratory testing performance. This approach provides critical insights for laboratory quality management and international accreditation.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of and Risk Factors Associated with Mono-Infections and Coinfections in Nongonococcal Urethritis.","authors":"Hangzhen Zhou, Feinan Cao, Xuefen Li, Yuejiao Dong, Xuying Yin","doi":"10.7754/Clin.Lab.2024.240929","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.240929","url":null,"abstract":"<p><strong>Background: </strong>Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), and Mycoplasma genitalium (MG) cause most cases of nongonococcal urethritis (NGU). The treatment options for different pathogens vary, and an accurate diagnosis of the pathogen species allows targeted treatment. This study explored the prevalence and etiology of coinfections and monoinfections with NGU.</p><p><strong>Methods: </strong>Neisseria gonorrhoeae (NG), CT, UU, and MG RNA were detected in urine and secretion samples of 1,430 patients with urethritis and 527 patients without urethritis (control group) using simultaneous amplification and testing. The corresponding clinical information was obtained. When these four pathogens were not detected, idiopathic urethritis (IU) was diagnosed.</p><p><strong>Results: </strong>Among the enrolled patients, 1,249 had NGU, 181 had gonococcal urethritis, and 28.26% (353/1,249) had IU. The average age of patients with NGU was 31 years. Additionally, 81.67% (1,020/1,249) of patients with NGU were men. Coinfections with NGU were observed in 13.05% (163/1,249) of patients, and they mainly comprised UU and CT. Among the patients with NGU, UU was the most common cause (40.59%; 507/1,249). Seventy-six patients with urethral symptoms were excluded from the control group. A comparison of the NGU and control groups revealed that NGU was caused by single UU, CT, and MG infections as well as coinfections. Single UU infections were the most common cause of NGU. The prevalence of single UU infections significantly exceeded that of single CT and MG infections. A follow-up analysis of 310 patients showed that the CT and UU infection rates significantly decreased after 1 to 2 weeks of treatment; however, the MG infection rate was not significantly reduced after at least 4 weeks of treatment.</p><p><strong>Conclusions: </strong>The incidence of NGU is significantly higher than that of gonorrhea, and coinfections are common in men with NGU. Furthermore, most men with NGU had UU coinfections. UU is the most common cause of NGU. Additionally, UU infections were commonly observed in young men without a history of NGU. Our results provide guidance for relevant clinical therapies.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Interference Effects of Ethamsylate on the Determination of Serum Creatinine Levels Using Two Methods.","authors":"Chun-Se Huang, Xian-Ming Liang, Yun-Hui Liao, Ping Chen, Qing-Yong Huang","doi":"10.7754/Clin.Lab.2024.241116","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241116","url":null,"abstract":"<p><strong>Background: </strong>Accurate quantification of serum creatinine is clinically essential for evaluating renal function. The aim of this study was to investigate the interference of ethamsylate in the detection of serum creatinine via the sarcosine oxidase method and the picric acid method.</p><p><strong>Methods: </strong>Thirty fresh serum samples with gradient concentrations of creatinine were collected from patients in our hospital. In accordance with CLSI EP7-A2, paired difference experiments and dose-response experiments were conducted to detect serum creatinine by the sarcosine oxidase method and the picric acid method, respectively. In addition, the interference of ethamsylate on the detection of serum creatinine at different concentrations was observed to compare the interference effect of ethamsylate on the detection of serum creatinine by the sarcosine oxidase method and picric acid method.</p><p><strong>Results: </strong>Paired difference tests revealed that 0.3 g/L ethamsylate significantly negatively affected the determination of creatinine at low, medium, and high creatinine concentrations via the sarcosine oxidase method, and the interference values were -53.67 μmol/L, -93.33 μmol/L, and -318.33 μmol/L, respectively. There was positive interference in the determination of low-concentration creatinine by the picric acid method; the interference value was 7.67 μmol/L, but there was no interference in the determination of medium- and high-concentration creatinine. Dose-response experiments revealed that the interference effect of ethamsylate on the determination of creatinine at low, medium, and high concentrations was a cubic regression curve. In the samples with low, medium, and high concentrations of creatinine, the lowest effective doses of ethamsylate interfering with the sarcosine oxidase method were 5.78 mg/L, 6.36 mg/L, and 9.66 mg/L, respectively. The lowest effective doses of ethamsylate that interfered with the low-, medium-, and high-creatinine samples via the picric acid method were 70.16 mg/L, 617.36 mg/L, and 859.19 mg/L, respectively. The interference pattern analysis suggests that the degree of interference diminished as creatinine concentration increased.</p><p><strong>Conclusions: </strong>Both the sarcosine oxidase method and the picric acid method are affected by ethamsylate. The picric acid method was relatively less effective and was effective mainly for creatinine samples at low concentrations.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}