Clinical laboratory最新文献

{"title":"Distribution of Pathogenic Bacteria and Analysis of Antimicrobial Susceptibility in Non-Lactation Granulomatous Mastitis Patients.","authors":"Hongye Ma, Yifei Zeng, Di Qu, Xiaoxia Huang, Xuanyu Wang, Xiang Gao, Honglin Guo","doi":"10.7754/Clin.Lab.2025.250235","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250235","url":null,"abstract":"<p><strong>Background: </strong>The aim was to comprehensively explore the distribution of pathogenic bacteria and antimicrobial susceptibility in the breast puncture fluid of the patients with non-lactation granulomatous mastitis to optimize treatment programs.</p><p><strong>Methods: </strong>Fifteen strains of pathogenic bacteria isolated from the breast puncture fluid of the patients with non-lactation granulomatous mastitis were collected in the breast department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University from March 2023 to October 2023. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA sequencing were used to bacterial identi-fication. The E-test method was used to determine the minimum inhibitory concentration (MIC) for each isolate, and the EUCAST standard was used for conducting the antimicrobial susceptibility interpretation. The control lipophilic experiment was performed by adopting the 1% Tween-80 blood plate.</p><p><strong>Results: </strong>The mean age of 15 patients with non-lactation granulomatous mastitis was 32.73 ± 3.37 years old, of which 14 (93.3%) had a history of pregnancy, and the mean postpartum mastitis was 6.38 ± 9.65 months. MALDL-TOF MS results were consistent with 16S rRNA sequencing by nanopore. Among the isolated fastidious bacteria, there were 15 strains of Corynebacterium kroppenstedtii (CK). These strains showed a high susceptibility rate to rifampicin and ceftriaxone (respectively, 93.3% and 80%). The resistance rate of penicillin was as high as 80%. The resistance rate of ciprofloxacin was 46.7%, and the intermediate rate was 53.3%. The sensitivity rate of moxifloxacin was 53.3%. There were no vancomycin-resistant or linezolid-resistant strains found.</p><p><strong>Conclusions: </strong>CK plays an important role in the occurrence and development of non-lactation granulomatous mastitis. According to the analysis of clinical antibiotic medication norms and drug sensitivity results, lipophilic rifampicin and linezolid may be the preferred empirical drugs for CK-related non-lactation granulomatous mastitis patients. Meanwhile, the clinical laboratory should endeavor to optimize the culture conditions and use MALDL-TOF MS for accurate identification to improve the detection rate of culture.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Variants in Pediatric Myeloproliferative Neoplasms Revealed by Next Generation Sequencing.","authors":"Chang-Hun Park, Heyjin Kim, Boram Kim, Jae Won Yun, Ki-O Lee, Keon Hee Yoo, Hyun-Young Kim, Hee-Jin Kim","doi":"10.7754/Clin.Lab.2025.250321","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250321","url":null,"abstract":"<p><strong>Background: </strong>Myeloproliferative neoplasms (MPNs) are clonal disorders of hematopoietic stem cells that include BCR::ABL1-negative MPNs such as essential thrombocythemia (ET) and primary myelofibrosis (PMF). MPNs are rare in children, and knowledge of the genetics and biology of pediatric MPNs is very limited. Here, we report genetic variants in pediatric MPNs revealed by next generation sequencing (NGS).</p><p><strong>Methods: </strong>The study included nine pediatric patients (eight with ET and one with PMF) consecutively diagnosed between January 2000 and June 2023. NGS was performed on an Ion S5 XL Sequencer with the OncomineTM myeloid research assay using bone marrow aspirate samples.</p><p><strong>Results: </strong>Five patients (56%) had clinically significant genetic variants. Two patients with ET had JAK2 V617F (driver) and two patients with ET had FLT3 E656A and ETV6 I10V, respectively. A single patient with PMF had 10 variants in eight genes, including two previously reported nonsense variants (ASXL1 W960* and TET2 R1452*) and three novel variants (BCOR A997E, TET2 I2002Mfs*12, and ZRSR2 F86_E102del). Of all, one patient (11%) experienced an event of transient ischemic attack with visual loss for 5 minutes and one patient with PMF expired of septic shock four months after diagnosis.</p><p><strong>Conclusions: </strong>The results suggest a different genetic profile in pediatric MPN, with a lower incidence of driver variants in pediatric ET and multiple non-driver variants with poor prognostic implications in pediatric PMF.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Resolution Genotyping of HLA Alleles and Association with Genetic Background of Virological Response in Chronic Hepatitis B.","authors":"Adriana Tălăngescu, Alexandru A Bratei, Maria Tizu, Bogdan Calenic, Alexandra-Elena Constantinescu, Ileana Constantinescu","doi":"10.7754/Clin.Lab.2025.250119","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250119","url":null,"abstract":"<p><strong>Background: </strong>Hepatitis B virus (HBV) infection is a major global health problem and can cause chronic infections and promote the development of cirrhosis and liver cancer. In the current investigation, we focused on evaluating the association between human leukocyte antigen (HLA) class I and II genotyping, HBV viral load, and the presence of steatosis in CHB (chronic hepatitis B) patients.</p><p><strong>Methods: </strong>In this study, we evaluated 204 patients with CHB who did not receive antiviral therapy before inclusion in the study and during the follow-up period. All patients were divided into 2 categories based on their HBV DNA levels. The presence of hepatic steatosis was determined by an ultrasound examination and SteatoTest. HLA genotyping for 11 genes, including HLA class I and class II, was conducted using next-generation sequencing.</p><p><strong>Results: </strong>Four HLA class II alleles, HLA-DQA1*01:02:02 (p = 0.019), HLA-DQB1*05:02:01 (p = 0.014), HLA-DRB1*16:01:01 (p = 0.032), and HLA-DRB5*02:02:01 (p = 0.052), were found to be positively associated with high levels of HBV-DNA. Furthermore, when studying the association of HLA class I and class II alleles with hepatic steatosis, our data showed that HLA-B*08:01:01 (p = 0.011), HLA-C*07:01:01 (p = 0.011), and HLA-DRB3*01:01:02 (p = 0.027) were positively correlated with the presence of hepatic steatosis.</p><p><strong>Conclusions: </strong>Integrating next-generation sequencing data of HLA genes in genomic and epigenomic data can offer a comprehensive understanding of the molecular mechanisms underlying HBV infection. This integrated approach will help identify new biomarkers, deeply understand the complex interactions between genetic and epigenetic factors, and facilitate the development of personalized prevention and treatment strategies.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune Checkpoint Inhibitor-Associated Colitis with Fever as the First Symptom.","authors":"Jingshan Bai, Xiaoning Zhang, Wanyang Xu, Tongtong Cui, Wenrui Li, Kunyang Li, Yi Chang","doi":"10.7754/Clin.Lab.2025.241251","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241251","url":null,"abstract":"<p><strong>Background: </strong>In recent years, the application of immunotherapeutic agents in the treatment of non-small cell lung cancer has advanced the current treatment landscape. However, side effects associated with immunotherapy should not be underestimated, as these effects can be life-threatening once they occur. In this article, we report a case of immune checkpoint inhibitor-related colitis presenting with fever as the initial symptom.</p><p><strong>Methods: </strong>Fibercolonoscopy, fiberbronchoscope.</p><p><strong>Results: </strong>In the case of this patient, fever initially developed after the administration of immune checkpoint inhibitors (ICIs), followed by severe diarrhea. The diagnosis of immune checkpoint inhibitor-related colitis was confirmed through fiber colonoscopy, and the patient's condition improved following treatment with glucocorticoids.</p><p><strong>Conclusions: </strong>For patients with advanced squamous cell lung cancer undergoing ICIs therapy, the early completion of an endoscopic evaluation to assess the severity of gastrointestinal symptoms, coupled with prompt initiation of steroid treatment, is crucial to improving outcomes.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case of Gitelman Syndrome Complicated by Growth Hormone Deficiency.","authors":"Guanwu Lu, Xiaoyu Liang, Tingguan Huang, Guansheng Wu","doi":"10.7754/Clin.Lab.2025.241246","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241246","url":null,"abstract":"<p><strong>Background: </strong>In September 2022, a case of Gitelman syndrome combined with growth hormone deficiency was diagnosed in the pediatrics department of our hospital. The patient was a 14-year-old male who was admitted to the hospital due to weakness in both lower extremities for two days and the symptoms had worsened within half a day. From 2016 to 2022, the patient had been hospitalized four times for hypokalemia. The clinical manifestations included weakness in both lower extremities, difficulty walking, muscle pain in the lower extremities and thirst.</p><p><strong>Methods: </strong>Blood and urine electrolyte tests, genetic testing for hereditary kidney diseases, and growth hormone stimulation tests were conducted.</p><p><strong>Results: </strong>Laboratory test results showed potassium (K+) at 1.90 mmol/L, magnesium (Mg) at 0.65 mmol/L, 24-hour urine calcium at 0.12 mmol/24 hour, pH at 7.454, PaCO2 at 44.5 mmHg, PaO2 at 90 mmHg, HCO3- at 31.2 mmol/L, and BE at 7 mmol/L. Genetic testing for hereditary kidney diseases revealed that the child and his mother carried a heterozygous nucleotide variation of the SLC12A3 gene, c.497C>T, resulting in a missense variation of p.Ala166Val; the father did not have this variation, and no large fragment variations of the SLC12A3 gene were found. The final diagnosis was Gitelman syndrome caused by a single heterozygous mutation. Additionally, the patient's height was 148 cm (below the third percentile, -2.49 SD), and the results of the growth hormone stimulation test indicated growth hormone deficiency. After a multidisciplinary consultation, the diagnosis was con-firmed as: 1. Gitelman syndrome (hypokalemia); 2. Growth hormone deficiency. Oral spironolactone tablets were given for potassium retention treatment, and subcutaneous injection of recombinant human growth hormone was recommended. The patient's condition improved, and regular follow-ups were advised.</p><p><strong>Conclusions: </strong>Case of Gitelman syndrome (GS) combined with growth hormone (GH) deficiency are relatively rare. This case enriches the understanding of concurrent symptoms of GS and is helpful for improving the clinical understanding and treatment level of this disease.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the Relationship between Macrophage-Related Proteins and the Risk and Prognosis of Breast Cancer.","authors":"Hong-Fang Ma, Qi-Na He, Yi Lu, Jun Shen","doi":"10.7754/Clin.Lab.2025.241128","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241128","url":null,"abstract":"<p><strong>Background: </strong>Macrophage-related proteins play a crucial role in breast cancer. The present study explored the relationship between macrophage-related proteins and breast cancer using Mendelian randomization (MR) for genetic variations and bioinformatics methods for transcriptomics.</p><p><strong>Methods: </strong>Genetic instruments associated with macrophage migration inhibitory factor (MIF), macrophage inflammatory protein 1α (MIP-1α), macrophage inflammatory protein 1β (MIP-1β), and granulocyte macrophage colony-stimulating factor (GM-CSF) were gathered from genome-wide association studies (GWAS). The MR analysis was conducted using R software packages 'TwoSampleMR' and 'MRPRESSO', employing MR-Egger, in-verse-variance weighted (IVW), weighted median, simple mode, and MR-PRESSO algorithms. In addition, data from the UCSC Xena database provided the TCGA BRCA dataset for a 5-year overall survival analysis of MIP-1α.</p><p><strong>Results: </strong>IVW analysis showed a significant positive association between MIP-1α and breast cancer incidence (OR = 1.0837, 95% CI: 1.0284 - 1.142), and the MR-PRESSO result also confirmed a causal relationship between them (OR = 1.0789, 95% CI: 1.0266 - 1.1338). There was no significant causal relationship found between MIF, MIP-1B, GM-CSF, and breast cancer. Survival analysis revealed that CCL3 was associated with prognosis in breast cancer patients in the Cox proportional-hazard model (HR = 1.5000, 95% CI: 1.0110 - 2.2250).</p><p><strong>Conclusions: </strong>Elevated levels of macrophage inflammatory protein 1A may increase the risk of breast cancer and lead to poorer patient outcomes.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GA/HbA1c Ratio Predicts the Occurrence of Diabetic Ketoacidosis and Disease Severity in Children with Type 1 Diabetes Mellitus.","authors":"Wei Wang, LiHua Qiu, TianTian Li, DongMei Sun, Qi Cao","doi":"10.7754/Clin.Lab.2024.241225","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2024.241225","url":null,"abstract":"<p><strong>Background: </strong>Pediatric type 1 diabetes mellitus (T1DM) is a significant endocrine condition, with diabetic ketoacidosis (DKA) posing a life-threatening risk. The aim of this study was to investigate the potential of the glycated albumin (GA) to glycosylated hemoglobin (HbA1c) ratio in predicting the occurrence of DKA and disease severity in children with T1DM.</p><p><strong>Methods: </strong>In this study, 224 children with T1DM were retrospectively analyzed and divided into non-DKA group (n = 142) and DKA group (n = 82). The patients' GA and HbA1c levels as well as other related biochemical indexes were detected, and the GA/A1c ratio was calculated and analyzed for its correlation with the occurrence of DKA and disease severity. Meanwhile, the value of GA/A1c ratio in predicting DKA was evaluated by using the receiver operating characteristic curve and the area under the curve.</p><p><strong>Results: </strong>In the DKA group, both GA and GA/A1c ratio were elevated, and patients with severe DKA had especially high GA/A1c ratio. GA/A1c ratio was effective in clinically predicting the occurrence and severity of DKA. Increased GA and GA/A1c ratio correlated with the high risk of DKA in T1DM patients. For T1DM patients, a greater GA/A1c ratio indicated a higher risk of DKA compared to GA. There was a linear relationship between GA/A1c ratio and DKA, and when the GA/A1c ratio was greater than 2.465, an increase in the GA/A1c ratio was positively correlated with a significant increase in the risk of developing DKA in T1DM.</p><p><strong>Conclusions: </strong>GA/A1c ratio can be used as an effective indicator to predict the occurrence of DKA and its severity in pediatric T1DM patients, and the higher the GA/A1c ratio, the higher the risk of DKA and the severity of the disease in patients.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obstetric Features of Large-Volume Red Blood Cell Transfusions in Parturients with Postpartum Hemorrhage: a Cross-Sectional Study.","authors":"Huimin Deng, Yuanshan Lu, Yanlin Du, Xiao Wang, Yiran Li, Zehua Dong, Meiting Li, Lan Hu, Xiaofei Yuan, Fengcheng Xu, Rong Xia, Shaoheng Chen","doi":"10.7754/Clin.Lab.2025.241249","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.241249","url":null,"abstract":"<p><strong>Background: </strong>Packed red blood cell (pRBC) transfusion is a common therapeutic intervention for the treatment of postpartum hemorrhage (PPH). This study aimed to describe obstetric characteristics related to large-volume pRBC transfusions in pregnant women with PPH and evaluate the effect of blood conservation strategies and maternal outcomes.</p><p><strong>Methods: </strong>This retrospective study included all parturients who received pRBC transfusions from 2016 - 2022 at a class A tertiary general hospital. Large-volume pRBC transfusions were defined as the receipt of ≥ 4 pRBC units during the postpartum period (within 24 hours). Numerous prenatal factors related to previous pregnancy/labor/ abortion, pregnancy complications, or antenatal laboratory indicators, as well as blood conservation strategies and maternal outcomes, were identified and compared.</p><p><strong>Results: </strong>Out of the 305 (305/24,997; 1.2%) women who received pRBC transfusions, 156 (51.1%) received ≥ 4 pRBC units during the postpartum period (within 24 hours). Women with large-volume pRBC transfusions had a greater prevalence of previous cesarean delivery (40.4%) and abortion (63.5%) than those who received 1 - 3 pRBC units (22.8% and 52.3%, respectively), as well as parturients with a history of placenta previa/accreta, placental adhesion, and cesarean delivery. In addition, those who received ≥ 4 pRBC units had greater incidences of thrombocytopenia and hypofibrinogenemia than those who received 1 - 3 pRBC units. In addition, parturients who received more pRBCs tended to receive more positive blood protection measures, including uterine balloon tamponade, embolization, and tranexamic acid administration, as well as having a longer length of hospitalization and more prolonged ICU stays.</p><p><strong>Conclusions: </strong>In this study, more than half of the parturients received ≥ 4 pRBC units during the postpartum period. Further studies that focus on identifying high‑risk pregnant women in prenatal settings could enable enrollment in a blood conservation program, therefore minimizing allogeneic blood transfusion (ABT)-related risks.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rare Case of AML-M1 with Positive Cup-Like Blast Cell.","authors":"Xiuping Xu, Chunxiao He","doi":"10.7754/Clin.Lab.2025.250130","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250130","url":null,"abstract":"<p><strong>Background: </strong>Acute myeloid leukemia (AML) is a clonal malignant tumor of hematopoietic stem cells. At present, MICM classification is commonly used in the world, in which morphology is the most basic and important. A bone marrow smear can inform clinicians of a variety of disease information, such as the presence of cup-like blast (CLB) cells often indicates AML with mutations in the NPM1 or FLT3-ITD genes.</p><p><strong>Methods: </strong>Blood routine and bone marrow routine tests were used to detect the cell proliferation condition and morphology in peripheral blood and bone marrow of patients.</p><p><strong>Results: </strong>Blood routine of the patient indicated a WBC of 176.33 x 109/L with 80% blasts. The bone marrow routine showed that blasts showed obvious hyperplasia, AML-M1 was considered for morphology. CLB cells are visible, and FLT3-ITD and NPM1 gene mutation detection was recommended.</p><p><strong>Conclusions: </strong>We investigated the association between CLB cells and FLT3-ITD/NPM1 gene mutation, as well as the value of CLB cells in diagnosis and treatment for AML by combining the case and relevant literature.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of a Near-Missing RhDV Type 1 Detection.","authors":"Haijuan Wang, Jian Chen, Guojin Ou","doi":"10.7754/Clin.Lab.2025.250139","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250139","url":null,"abstract":"<p><strong>Background: </strong>The safety of blood transfusion can be significantly affected by complex blood group antigens, especially the polymorphic Rh blood group system. This case study is to alert clinicians and laboratory personnel to accurately identify and manage RhD blood groups.</p><p><strong>Methods: </strong>A Chinese female patient with cervical squamous cell carcinoma underwent surgical treatment; her blood typing identified that she had serological type A and different RhD titers to the anti-D reagent. RHD genetic testing was performed.</p><p><strong>Results: </strong>After RHD gene sequencing, the patient was confirmed as RhDV Type 1; 1.5 units of type A RhD-negative suspended red blood cells were transfused to the patient, and the hemoglobin level increased from 66 to 73 g/L. No adverse reaction occurred, and the patient recovered well after surgery.</p><p><strong>Conclusions: </strong>RhD antibody reagents from different manufacturers are required to identify the RhD subtypes and ensure transfusion safety.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"71 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}