Brazilian dental journalPub Date : 2024-12-06eCollection Date: 2024-01-01DOI: 10.1590/0103-644020235851
Marcio Alex Barros Gomes, Camilla Christian Gomes Moura, Lucas Raineri Capeletti, Gustavo Silva Chaves, Daniel de Almeida Decurcio, Monise de Paula Rodrigues, Carlos José Soares
{"title":"Effect of Fiberglass Post Technique Removal on the Root Dentin Loss, the Root Canal Alignment and the Root Perforation Occurrence.","authors":"Marcio Alex Barros Gomes, Camilla Christian Gomes Moura, Lucas Raineri Capeletti, Gustavo Silva Chaves, Daniel de Almeida Decurcio, Monise de Paula Rodrigues, Carlos José Soares","doi":"10.1590/0103-644020235851","DOIUrl":"10.1590/0103-644020235851","url":null,"abstract":"<p><p>Root canal retreatment and fracture of fiberglass posts (FGP) can require the FGP removal. The aim of this study was to evaluate the effect of the FGP removal protocol on the time for FGP removal (min), the root dentin removed (mm3), the angle alteration of the root canal alignment (o), and the root perforation occurrence using cone-beam computed tomography (CBCT) analysis. Thirty extracted maxillary molars were randomly assigned to 3 groups (n = 10): Mic-Ult, FGP removed using ultrasonic inserts under microscopic magnification; Mic-DiB, FGP removed using diamond burs under microscopic magnification, and Endo-G, FGP removed using Endo-G. CBCTs were made after access opening and after FGP removal. The volume of root dentin, the root canal alignment and root perforation were performed using InVesalius and Mimics softwares. Root canal angle alteration after FGP removal was performed using ImageJ software. The time for FGP removal was calculated in min. Data were analysed by one-way analysis of variance was followed by Tukey HSD test (α = 0.05). Endo-G resulted in significantly lower root dentin removal (p< .001), and significantly less time than Mic-Ult and Mic-DiB (p< .001). Mic-DiB had 1 and Mic-Ult had 2 root dentin perforations. FGP post removal using Mic-Ult and Mic-DiB exhibited significantly greater alteration in the root canal alignment than that using Endo-G (p< .001). FGP removal using Endo-G exhibited better preservation of the original canal alignment, saved root dentin structure, and also required less time compared to FGP post removal using Mic-DiB or Mic-Ult under microscopic magnification.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e235851"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-12-06eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202405878
Lucas S Chaves, Daniel A Decurcio, Ana Paula R Magalhães, Gustavo S Chaves, Lucas Ra Estrela, Cyntia Ra Estrela, Carlos Estrela
{"title":"Influence of Dentin Hybridization on the Chromatic Alteration of Teeth caused by Endodontic Sealers.","authors":"Lucas S Chaves, Daniel A Decurcio, Ana Paula R Magalhães, Gustavo S Chaves, Lucas Ra Estrela, Cyntia Ra Estrela, Carlos Estrela","doi":"10.1590/0103-6440202405878","DOIUrl":"10.1590/0103-6440202405878","url":null,"abstract":"<p><p>This study evaluated the influence of dentin hybridization on coronary chromatic alteration of endodontically treated bovine teeth using different endodontic sealers and Portland cement. The 200 central incisors were endodontically treated using different materials (Sealapex, Bio-C Sealer, AH-Plus, Endofill, and Portland cement) and distributed according to the presence or absence of dentin hybridization. The teeth underwent colorimetric analysis using the Easyshade® spectrophotometer at four different times: determining the color of the dental substrate before root canal filling (RCF), 7 days, 60 days, and 180 days after RCF. The data were evaluated for normality and homogeneity by the Shapiro-Wilk and Levene tests. A two-way analysis of variance was performed, with Sidak's post-test for multiple comparisons. The significance level was 5%. Dentin hybridization influenced the coronary chromatic alteration of Sealapex® after 7 days and in Bio-C Sealer® and AH Plus® after 60 days. The AH Plus after 7 days obtained the highest ΔE* when hybridized, and the Endofill® obtained the highest ΔE* when not hybridized. The AH Plus after 60 days got the highest ΔE* when hybridized and non-hybridized. The Sealapex® after 180 days obtained the highest ΔE* when hybridized, and the AH Plus obtained the highest ΔE* when not hybridized. The studied root canal sealers behaved differently regarding coronary chromatic alteration, making it difficult to predict a particular behavior. All endodontic sealers caused clinically noticeable chromatic changes after 7 days, 60 days, and 180 days, regardless of hybridization.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245878"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-12-02eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202406015
Yani Corvianindya Rahayu, Ernie Maduratna Setiawatie, Retno Pudji Rahayu, S Siswandono, Retno Indrawati, Hendrik Setia Budi, Hari Basuki Notobroto, Rahmah A Alwasilah Darojah
{"title":"Anti-Osteoclastogenesis Potential of Cocoa Pod Husk (Theobroma cacao L.) Extract: In Silico and In Vivo Study.","authors":"Yani Corvianindya Rahayu, Ernie Maduratna Setiawatie, Retno Pudji Rahayu, S Siswandono, Retno Indrawati, Hendrik Setia Budi, Hari Basuki Notobroto, Rahmah A Alwasilah Darojah","doi":"10.1590/0103-6440202406015","DOIUrl":"10.1590/0103-6440202406015","url":null,"abstract":"<p><p>Periodontitis is a common chronic inflammatory disease characterized by alveolar bone loss. The high polyphenol content in cocoa pod husk (Theobroma cacao L) has the potential to influence bone metabolism and contribute to the inhibition of bone resorption. The aim of this study was to analyze the anti-osteoclastogenesis potential of cocoa pod husk (Theobroma cacao L.) in both in silico and in vivo study. An analysis of the anti-osteoclastogenesis potential of T. cacao bioactive compounds was conducted using molecular docking simulations. Thirty male Wistar rats (Rattus novergicus) were randomly assigned to control negative groups (placebo gel), control positive groups (2% doxycycline gel), and treatment groups (10% cocoa pod husk (CPH) extract gel), with measurements taken on days 7 and 14. Wistar rats were induced with 0.05 ml of P. gingivalis at a concentration of 2x109 CFU/ml intrasulcularly in the maxillary molar to achieved in periodontitis. The number of osteoclasts was observed by hematoxylin and eosin staining, the level of TNF-α was assessed by enzyme-linked immunosorbent assay, and the expression of RANKL was evaluated by immunohistochemical staining. Data were analyzed using One-way ANOVA to examine the differences between the groups. The in silico study showed that the catechin, epicatechin, quercetin, and procyanidin B2 had a strong binding affinity for TNF-α and RANKL. Administration of 10% CPH reduced the number of osteoclasts (p<0.05), TNF-α level on days 7 and 14 (p<0.05), and RANKL expression on day 7 (p<0.05) in experimental rats with periodontitis. Administering 10% CPH inhibited osteoclastogenesis in the experimental periodontitis rats.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246015"},"PeriodicalIF":0.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-10-28eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202405500
Diana Roberta Pereira Grandizoli, Letícia Oba Sakae, Ana Luísa Meira Renzo, Sávio José Cardoso Bezerra, Samira Helena Niemeyer, Taís Scaramucci
{"title":"The Effect of Actives in Desensitizing and Conventional Mouth Rinses Against Dentin Erosive Wear.","authors":"Diana Roberta Pereira Grandizoli, Letícia Oba Sakae, Ana Luísa Meira Renzo, Sávio José Cardoso Bezerra, Samira Helena Niemeyer, Taís Scaramucci","doi":"10.1590/0103-6440202405500","DOIUrl":"10.1590/0103-6440202405500","url":null,"abstract":"<p><p>This study evaluated the effect of actives present in conventional and desensitizing mouth rinses on the control of dentin erosive tooth wear. Two hundred and seventy dentin specimens from human molars were prepared. The specimens were randomly allocated into 10 experimental groups (n=10): 4 corresponding to desensitizing mouth rinses, 4 to conventional mouth rinses, a negative control group (C-: distilled water), and a positive control group (C+: 500 ppm fluoride plus 800 ppm tin mouth rinse). Specimens were subjected to an erosion-abrasion cycling model: 5 min immersion in 0.3% citric acid and 60 min exposure to artificial saliva. This procedure was repeated 4x/day for 5 days. Immediately after the first and last erosive challenges, the specimens were brushed with a slurry of fluoride toothpaste for 15 s, with a total of 2 min exposure to the slurry. Afterward, the specimens were exposed to the mouth rinses. Dentin surface loss (SL, in µm) was determined by optical profilometry. Data were statistically analyzed by using Kruskal-Wallis and Dunn's tests (α=0.05). The only mouth rinses that presented significantly lower dentin SL than the C- was a desensitizing one containing sodium fluoride (220 ppm F-) with dipotassium oxalate (1.4%) and the C+ (p<0.001 and p=0.013, respectively), without significant differences between them (p>0.05). Dentin SL of the other groups did not significantly differ from the C- (p>0.05). The combination of sodium fluoride with dipotassium oxalate in a desensitizing mouth rinse showed a promising result against dentin erosive wear, matching the protection offered by a fluoride/tin mouth rinse.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245500"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-10-28eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202406081
Carlos Estrela, Marcela Gimenes B Oshita, Matheus F Perazzo, Ana Helena G Alencar, Júlio A Silva, Lucas Ra Estrela, Luciano Ta Cintra, Cyntia Ra Estrela
{"title":"Quality of Communication Between Professors and University Students in the Process of Learning.","authors":"Carlos Estrela, Marcela Gimenes B Oshita, Matheus F Perazzo, Ana Helena G Alencar, Júlio A Silva, Lucas Ra Estrela, Luciano Ta Cintra, Cyntia Ra Estrela","doi":"10.1590/0103-6440202406081","DOIUrl":"10.1590/0103-6440202406081","url":null,"abstract":"<p><p>Teaching requires the incorporation of communication skills, and these potentials may affect the outcomes of the learning process. This study evaluated the quality of communication between professors and students during the process of learning Dentistry. A questionnaire was developed and applied to evaluate their perceptions about their communications in an undergraduate Dentistry course. The questionnaire had ten items to analyze professors' self-perception and ten for students' perceptions, using a Likert-like scale and a final open question. During its construction, it was sent to five specialists to analyze content validity. The open question gathered suggestions to improve and intensify communications effectively and to identify vulnerabilities. Total scores ranged from 10 to 50, with 10 as the worst perception, and 50, as the best. The scores were calculated by adding all answers and then dividing that sum by the total number of items. Cronbach's alpha was calculated to evaluate the instrument's internal consistency. The level of significance was set at 0.05%. The Student t-test was used to determine differences between groups. Professors' self-perceptions and students' perceptions of the quality of communication of the university teaching staff during the student's learning process had statistically significant differences. Professors classified their ability to communicate when emitting and receiving information as satisfactory. Students, however, did not fully agree with them, particularly on the items about receiving information. The perceptions identified in this study may lead to a new direction in the communicative behavior of professors and students.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246081"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-10-28eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202406021
Julia Menezes Savaris, Luiz Fernando Monteiro Czornobay, Maria Eduarda Paz Dotto, Pablo Silveira Santos, Lucas da Fonseca Roberti Garcia, Filipe Colombo Vitali, Cleonice da Silveira Teixeira
{"title":"Tooth Discoloration Induced by Endodontic Sealers of Different Chemical Bases: A Systematic Review.","authors":"Julia Menezes Savaris, Luiz Fernando Monteiro Czornobay, Maria Eduarda Paz Dotto, Pablo Silveira Santos, Lucas da Fonseca Roberti Garcia, Filipe Colombo Vitali, Cleonice da Silveira Teixeira","doi":"10.1590/0103-6440202406021","DOIUrl":"10.1590/0103-6440202406021","url":null,"abstract":"<p><p>The present study aimed to assess the tooth discoloration induced by endodontic sealers and establish a correlation between their distinct chemical compositions and this aesthetic concern. Five electronic databases and grey literature were systematically screened to identify studies comparing the tooth discoloration potential of endodontic sealers with different chemical bases. Studies that did not evaluate human teeth or did not employ spectrophotometry as a method for color measurement were excluded. The risk of bias in the included studies was assessed using a modified version of the JBI Critical Appraisal Checklist for Experimental Studies. Data were grouped according to the chemical composition of the sealers and analyzed qualitatively. Fourteen studies were included. None of the studies met all domains of the risk of bias checklist. Qualitative synthesis suggested that zinc oxide and eugenol-based sealers have a greater potential for tooth discoloration. Silicone-based and calcium hydroxide-based sealers demonstrated less potential for tooth discoloration than resin-based sealers and salicylate resin-based sealers containing calcium silicate. All investigated endodontic sealers induced tooth discoloration, which is chemical and time dependent. Zinc oxide and eugenol-based sealers exhibited a greater potential for tooth discoloration, whereas silicone and calcium hydroxide-based sealers showed less potential.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246021"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Positioning Guide Color on Shade Measurements Using a Clinical Spectrophotometer.","authors":"Joao Arthur Dumke, Lauren Arrua Fantine, Suzana Nogueira, Bruna Gaidarji, Danielle Zorzo Righes, Bibiana Gabardo Perez Mariano da Rocha, Leticia Brandao Durand","doi":"10.1590/0103-6440202405995","DOIUrl":"10.1590/0103-6440202405995","url":null,"abstract":"<p><p>The purpose of this study was to investigate the influence of the color of positioning guides on the CIEL*a*b* color coordinates of ceramic, resin composite and bovine tooth surfaces measured by a clinical spectrophotometer. Positioning guides (n=10) in different colors (translucent, purple, yellow, green and, blue) were made for each surface (ceramic, resin composite and bovine tooth). The CIEL*a*b* color coordinates were measured with the positioning guides and with no positioning guide (control group). The ΔL*, Δa*, Δb*, ΔE*ab, and ΔE00 were calculated between the control group and the different groups. The CIEL*a*b* color coordinates were analyzed by one-way ANOVA followed by the post-hoc Tukey test (α=0.05). Color differences were interpreted using the 50:50% perceptibility and acceptability visual thresholds. Positioning guides with different colors presented significant differences for all surfaces and color coordinates. ΔE*ab, and ΔE00 mean values for all surfaces and positioning guide colors exceeded the acceptability visual thresholds, except for the bovine tooth surface measured with the green positioning guide. The shade measurement was affected by the color of the positioning guides regardless of the surface that was evaluated.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245995"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-10-28eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202405793
Alexandra Mena-Serrano, Sandra Sanchez, María G Granda-Albuja, Michael Willian Favoreto, Taynara de Souza Carneiro, Deisy Cristina Ferreira Cordeiro, Alessandro D Loguercio, Alessandra Reis
{"title":"Hydrogen Peroxide in the Pulp Chamber and Color Change in Maxillary Anterior Teeth After In-Office Bleaching.","authors":"Alexandra Mena-Serrano, Sandra Sanchez, María G Granda-Albuja, Michael Willian Favoreto, Taynara de Souza Carneiro, Deisy Cristina Ferreira Cordeiro, Alessandro D Loguercio, Alessandra Reis","doi":"10.1590/0103-6440202405793","DOIUrl":"10.1590/0103-6440202405793","url":null,"abstract":"<p><p>While the literature has noted variations in hydrogen peroxide (HP) permeability, and consequently, tooth sensitivity among different types of anterior teeth, there is a scarcity of research on this specific topic. This study evaluated HP permeability and color change (CC) in different groups of human maxillary anterior teeth (canines, lateral incisors, and central incisors) after in-office bleaching with HP at 35%. Thirty teeth maxillary anterior (n=10) were bleached with HP at 35% gel in one session of three 15-minutes applications. Buccal thickness (mm) was measured from images obtained using cone beam computed tomography. The concentration of HP (µg/mL) that reached the pulp chamber was assessed using UV-Vis spectrophotometry. CC (WID, ∆Eab, and ∆E00) was evaluated before and after bleaching with a digital spectrophotometer. One-way ANOVA and Tukey's test were applied to statistically evaluate the data for buccal thickness, HP permeability, and CC (α=0.05). Comparison between thickness and HP permeability was performed using Pearson's correlation. Thicker teeth, such as canines, had lower HP amounts inside the pulp chamber compared to central and lateral incisors. Despite the significant effect of buccal thickness on HP permeability (p<0.05), no correlation was found between the two factors. CC was similar across tooth types (p>0.05). The difference in buccal thickness among the superior anterior teeth does not interfere with CC. However, a thinner buccal wall thickness is associated with greater HP permeability detected in the pulp after in-office bleaching.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245793"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-10-28eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202405763
Guilherme Gonçalves da Cruz, Roberta de Oliveira Alves, Caroline Garcia Orsi, André Luis Faria-E-Silva, Suzane Cristina Pigossi, Priscilla Barbosa Ferreira Soares
{"title":"Effect of Periodontal Instrumentation on Tooth Structure Exposure to Ionizing Radiation: In Vitro Study.","authors":"Guilherme Gonçalves da Cruz, Roberta de Oliveira Alves, Caroline Garcia Orsi, André Luis Faria-E-Silva, Suzane Cristina Pigossi, Priscilla Barbosa Ferreira Soares","doi":"10.1590/0103-6440202405763","DOIUrl":"10.1590/0103-6440202405763","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of different scaling protocols on the morphology and roughness of the root dentin substrate exposure to ionizing radiation. One hundred and thirty extracted bovine incisors were randomly divided into two groups (n=65): non-irradiated (NIR) and irradiated (IR). Each group was initially subdivided into three subgroups according to the type of non-surgical periodontal protocol: NIT: no instrumentation; HS: hand scaling with 15 apical-coronal instrument movements; US: ultrasonic scaling with 15 apical-coronal cycles. Subsequently, all samples were subjected to the prophylaxis protocol, being subdivided into the following groups: NIT/PP: prophylaxis with a fine prophylactic paste using a rubber cup for 15 seconds; HS/PP: Hand scaling followed by the prophylaxis protocol; US/PP: Ultrasonic scaling followed by the prophylaxis protocol. The roughness of the root dentin surface was measured with a profilometer (Ra/Rz - μm), and the morphology of the dentin surfaces was analyzed using scanning electron microscopy (SEM). The analyses were conducted before and after the prophylaxis protocol. In the absence of prophylaxis, the roughest surfaces were observed after ultrasonic instrumentation followed by hand instrumentation for both IR and NIR groups. No difference in Ra and RZ values between HS/PP and US/PP was observed for both substrates. For the IR group, the prophylaxis resulted in similar Ra and RZ values for both instrumentation groups in comparison to no instrumentation. Ordinal logistic regression showed that both HS and US resulted in higher scores than NIT, irrespective of IR presence. In conclusion, the IR showed a rougher root surface for both HS and US in comparison to NIR. However, the prophylaxis procedure significantly reduced the roughness of root surfaces after both instrumentation procedures.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245763"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-10-28eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202405786
Talita Caira Silva, Lais M Cardoso, Taisa N Pansani, Edson Alfredo, Carlos de Souza-Costa, Fernanda Gonçalves Basso
{"title":"Effect Of Different Alkaline Treatments of Titanium Surface on Human Osteoblasts Metabolism.","authors":"Talita Caira Silva, Lais M Cardoso, Taisa N Pansani, Edson Alfredo, Carlos de Souza-Costa, Fernanda Gonçalves Basso","doi":"10.1590/0103-6440202405786","DOIUrl":"10.1590/0103-6440202405786","url":null,"abstract":"<p><p>This investigation demonstrates the effect of alkali modification of titanium on the metabolism of human osteoblasts. Polished titanium discs were subjected to alkalinization protocols with NaOH (5M) at 60°C or 120°C. Surface topography and roughness were evaluated using scanning electron microscopy (SEM). Osteoblasts were seeded onto titanium discs, followed by cell adhesion and viability analysis, total protein and collagen production, and alkaline phosphatase (ALP) activity. Gene expression of tumor necrosis factor-alpha (TNF-α) and beta-defensin 3 (HBD3) was evaluated after inflammatory stimulus with lipopolysaccharides (LPS) of Porphyromonas gingivalis (1 μg/mL) for 4 h. Discs subjected to modification with NaOH showed major irregularities, especially for 120°C-protocol. Increased adhered cell number was observed for surfaces modified by NaOH. Osteoblasts cultured on modified surfaces showed higher cell viability, total protein and collagen synthesis, and ALP activity than that of cells cultured on the polished discs. Osteoblast response to LPS exposure showed increased TNF-α gene expression by these cells when cultured on the polished discs, while increased expression of HBD3 was detected for all groups in the presence of LPS. Modification of titanium discs by NaOH at 60°C or 120°C promoted an increase in adhesion and metabolism of osteoblasts and favored the response to inflammatory stimulus.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245786"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}